CN102565313B - Method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves - Google Patents
Method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves Download PDFInfo
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- CN102565313B CN102565313B CN 201110420458 CN201110420458A CN102565313B CN 102565313 B CN102565313 B CN 102565313B CN 201110420458 CN201110420458 CN 201110420458 CN 201110420458 A CN201110420458 A CN 201110420458A CN 102565313 B CN102565313 B CN 102565313B
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Abstract
The invention relates to a method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves after removing leaves. The method comprises the following steps of: taking 36-48 plants of lolium multiflorum which are consistent in growth and 3 years old, culturing for 7 weeks under the conditions that the temperature is 18-25 DEG C and the illumination time is 6-8 hours every day, respectively detecting the average carbon storage Cr in the root parts of the lolium multiflorum before removing the leaves, the average carbon storage Cs in stubbles of the lolium multiflorum before removing the leaves and the total average carbon storage Cn in regenerated leaf blades of the lolium multiflorum, and calculating the relative maximum amount RI of the stored carbohydrates capable of being utilized for regeneration of the lolium multiflorum leaves. According to the method for measuring the stored carbohydrates capable of being utilized for regenerationof the lolium multiflorum leaves, provided by the invention, the measuring method is simple, the relative maximum amount of the stored carbohydrates which can be utilized for regeneration of the leafblades of the lolium multiflorum before removing the leaves can be detected, the process is simple, the operation is simple, and the production efficiency and the working benefits can be further improved.
Description
Technical field
The present invention relates to a kind of measuring method, specifically a kind of method of measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves.
Background technology
Herbage searched for food by plant-eating animal or the people for cut cut after, usually show " reproducibility ", this is that herbage is kept its own existence, resists one of the most basic biological characteristics of plant-eating animal injury, and it is produced keeping the grassland, the raising grass yield also has important meaning.The regeneration of herbage is one and relates to many-sided, comparatively complicated problem, but numerous regeneration problems must face a basic fact, the source problem that comes of the required carbohydrates of namely regenerating behind the defoliation.Because the essential problem that herbage grows again is a process of utilizing energy and material to make newborn organic substance, yet the herbage defoliation not only can its photosynthesis of severe inhibition, reduces the supply of photosynthate, even in the situation that defoliation is serious, can cause light action to interrupt fully.So, the photosynthetic organic product of storing in the herbage defoliation precursor, namely the parent carbohydrates might produce very important effect to its regeneration.
But present numerous scholar is to the research of pasture utilization carbohydrates, only limit to produce wholesome effect to regeneration from clear and definite its in aspect of matter, or the carbohydrates that utilizes isotope technology checking regeneration to utilize has part to come from the carbohydrates of storing before the defoliation.Make the carbohydrates of storing before the herbage defoliation clear and have on earth in the growth course that how much participates directly in the regeneration blade, the regeneration of research herbage is had great importance, yet yet there are no so far report about the concrete measuring method of this respect.
Summary of the invention
The present invention is directed to the deficiency of the problems referred to above, the method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves is provided, its measuring method is simple, can detect the relative maximum of the carbohydrates of the front storage of its defoliation that the regeneration of rye blade of grass can utilize.
The present invention addresses the above problem the technical scheme that adopts to be: measure the method for stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves, its measuring method is:
Step 1, choose consistent 3 week Itanlian rye 36-48 strains in age of growth, implant in 6 identical flowerpots with every basin 6-8 strains, mixed-matrix is housed in the flowerpot, mixed-matrix is comprised of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, be 17-25 ℃ in temperature, illumination every day is under 6-8 hours conditions, cultivated for 7 weeks, cut off the blade of Itanlian rye, all Itanlian ryes all stay identical stubble high, and stubble is high to be 1-5cm, then 6 basin Itanlian ryes are divided into 2 groups at random, every group of 3 basins, namely test group a and test group b are for subsequent use;
Step 2, get the Itanlian rye among the test group a, the root water of Itanlian rye in every basin is washed out, after the root of Itanlian rye is separated with stubble, obtain Itanlian rye root and Itanlian rye stubble, Itanlian rye root and Itanlian rye stubble sent in 75 ℃ the drying baker, oven dry, obtain every basin Itanlian rye root oven dry thing and Itanlian rye stubble oven dry thing, detect biomass and carbon content in every basin Itanlian rye root and the Itanlian rye stubble oven dry thing, after calculate the average carbon reserves in the Itanlian rye stubble before the average carbon reserves of Itanlian rye root before the defoliation and the defoliation, for subsequent use;
Step 3, be under 20-25 ℃ of conditions with the Itanlian rye among the test group b in temperature, carrying out lucifuge cultivates, every 6 days, cut newborn Itanlian rye blade in the step 1 institute high position of toothing, until all Itanlian ryes among the test group b are ended when stopping growing; The Itanlian rye blade of at every turn cutting sent in 75 ℃ the drying baker, oven dry, detect biomass and carbon content in the oven dry thing, obtain the carbon reserves of every basin Itanlian rye regeneration blade behind each defoliation, calculate every basin regeneration blade total carbon reserves, obtain at last the average total carbon reserves of Itanlian rye regeneration blade, for subsequent use;
Step 4, utilize the average carbon reserves C of Itanlian rye root before the defoliation
r, the average carbon reserves C before the defoliation in the Itanlian rye stubble
sAverage total carbon reserves C with Itanlian rye regeneration blade
n, according to formula RI=
Calculate the relative maximum RI of stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves.
Beneficial effect
A, measuring method of the present invention are simple, and easy operating is convenient to widespread use, and practical.
B, the present invention detect the relative quantity that regeneration rye blade of grass utilizes storage carbohydrate by measuring carbon, and when detecting carbon content, required instrument is simple, and is with low cost, improved economic benefit.
Embodiment
Measure the method for stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves, its measuring method is:
Step 1, choose consistent 3 week Itanlian rye 36-48 strains in age of growth, implant in 6 identical flowerpots with every basin 6-8 strains, mixed-matrix is housed in the flowerpot, mixed-matrix by ratio of weight and the number of copies is by 3 parts of vermiculites and 1 part of turfy soil preparation, be 17-25 ℃ in temperature, under 6-8 hours conditions of illumination every day, after cultivating for 7 weeks, cut off the blade of Itanlian rye, all Itanlian ryes all stay identical stubble high, and stubble is high to be 1-5cm, then 6 above-mentioned basin Itanlian ryes are divided into 2 groups at random, every group of 3 basins, namely test group a and test group b are for subsequent use;
Step 2, get the Itanlian rye among the test group a, the root water of Itanlian rye in every basin is washed out, after separate with stubble again, obtain Itanlian rye root and stubble, above-mentioned Itanlian rye root and stubble are sent in 75 ℃ the drying baker, oven dry, obtain every basin Itanlian rye root and stubble oven dry thing, detect biomass and carbon content in every basin Itanlian rye root and the stubble oven dry thing, after calculate the average carbon reserves in the Itanlian rye stubble before the average carbon reserves of Itanlian rye root before the defoliation and the defoliation, for subsequent use;
Step 3, the Itanlian rye among the test group b is carried out lucifuge cultivate under 20-25 ℃ condition, every 6 days, cut newborn Itanlian rye blade in the step 1 institute high position of toothing, until all Itanlian ryes among the test group b are ended when all stopping growing; The above-mentioned Itanlian rye blade of at every turn cutting sent in 75 ℃ the drying baker, oven dry, detect biomass and the carbon content of oven dry thing, obtain the carbon reserves of every basin Itanlian rye regeneration blade behind each defoliation, calculate afterwards every basin regeneration blade total carbon reserves, calculate again the average total carbon reserves of Itanlian rye regeneration blade, for subsequent use;
Step 4, utilize the average carbon reserves C of Itanlian rye root before the defoliation
r, the average carbon reserves C before the defoliation in the Itanlian rye stubble
sAverage total carbon reserves C with Itanlian rye regeneration blade
n, according to formula RI=
Calculate the relative maximum RI of stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves.
Embodiment 1
Measure the method for stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves, its measuring method is:
Step 1, choose consistent 3 week Itanlian rye 36 strains in age of growth, implant in 6 identical flowerpots with every basin 6 strains, mixed-matrix is housed in the flowerpot, mixed-matrix is comprised of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, it is 17 ℃ in temperature, illumination every day is under 6 hours conditions, cultivated for 7 weeks, cut off the blade of Itanlian rye, all Itanlian ryes all stay identical stubble high, and stubble is high to be 1cm, then 6 basin Itanlian ryes are divided into 2 groups at random, every group of 3 basins, namely test group a and test group b are for subsequent use;
Step 2, get the Itanlian rye among the test group a, the root water of Itanlian rye in every basin is washed out, after the root of Itanlian rye is separated with stubble, obtain Itanlian rye root and Itanlian rye stubble, Itanlian rye root and Itanlian rye stubble sent in 75 ℃ the drying baker, oven dry, obtain every basin Itanlian rye root oven dry thing and Itanlian rye stubble oven dry thing, detect biomass and carbon content in every basin Itanlian rye root and the Itanlian rye stubble oven dry thing, after calculate the average carbon reserves in the Itanlian rye stubble before the average carbon reserves of Itanlian rye root before the defoliation and the defoliation, for subsequent use;
Step 3, be under 20 ℃ of conditions with the Itanlian rye among the test group b in temperature, carry out lucifuge and cultivate, every 6 days, cut newborn Itanlian rye blade in the step 1 institute high position of toothing, cut 3 blades after Itanlian rye namely stopped growth; The Itanlian rye blade of at every turn cutting sent in 75 ℃ the drying baker, oven dry, detect biomass and carbon content in the oven dry thing, obtain the carbon reserves of every basin Itanlian rye regeneration blade behind each defoliation, calculate every basin regeneration blade total carbon reserves, obtain at last the average total carbon reserves of Itanlian rye regeneration blade, for subsequent use;
Step 4, utilize the average carbon reserves C of Itanlian rye root before the defoliation
r, the average carbon reserves C before the defoliation in the Itanlian rye stubble
sAverage total carbon reserves C with Itanlian rye regeneration blade
n, according to formula RI=
Calculate the relative maximum RI of stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves;
The result shows, RI is 20.35%, namely at jointing in the time of front 20 days, leaves that stubble is high be the Itanlian rye of 1cm, and the relative maximum of the carbohydrates of storage is 20.35% in its body that leaf regeneration utilizes.
Embodiment 2
Measure the method for stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves, its measuring method is:
Step 1, choose consistent 3 week Itanlian rye 42 strains in age of growth, implant in 6 identical flowerpots with every basin 7 strains, mixed-matrix is housed in the flowerpot, mixed-matrix is comprised of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, it is 21 ℃ in temperature, illumination every day is under 7 hours conditions, cultivated for 7 weeks, cut off the blade of Itanlian rye, all Itanlian ryes all stay identical stubble high, and stubble is high to be 3cm, then 6 basin Itanlian ryes are divided into 2 groups at random, every group of 3 basins, namely test group a and test group b are for subsequent use;
Step 2, get the Itanlian rye among the test group a, the root water of Itanlian rye in every basin is washed out, after the root of Itanlian rye is separated with stubble, obtain Itanlian rye root and Itanlian rye stubble, Itanlian rye root and Itanlian rye stubble sent in 75 ℃ the drying baker, oven dry, obtain every basin Itanlian rye root oven dry thing and Itanlian rye stubble oven dry thing, detect biomass and carbon content in every basin Itanlian rye root and the Itanlian rye stubble oven dry thing, after calculate the average carbon reserves in the Itanlian rye stubble before the average carbon reserves of Itanlian rye root before the defoliation and the defoliation, for subsequent use;
Step 3, be under 23 ℃ of conditions with the Itanlian rye among the test group b in temperature, carry out lucifuge and cultivate, every 6 days, cut newborn Itanlian rye blade in the step 1 institute high position of toothing, cut 3 blades after Itanlian rye namely stopped growth; The Itanlian rye blade of at every turn cutting sent in 75 ℃ the drying baker, oven dry, detect biomass and carbon content in the oven dry thing, obtain the carbon reserves of every basin Itanlian rye regeneration blade behind each defoliation, calculate every basin regeneration blade total carbon reserves, obtain at last the average total carbon reserves of Itanlian rye regeneration blade, for subsequent use;
Step 4, utilize the average carbon reserves C of Itanlian rye root before the defoliation
r, the average carbon reserves C before the defoliation in the Itanlian rye stubble
sAverage total carbon reserves C with Itanlian rye regeneration blade
n, according to formula RI=
Calculate the relative maximum RI of stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves;
The result shows, RI is 23.53%, namely at jointing in the time of front 20 days, leaves that stubble is high be the Itanlian rye of 3cm, and the relatively amount of maximum of the carbohydrates of storing in its body that leaf regeneration utilizes is 23.53%.
Embodiment 3
Measure the method for stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves, its measuring method is:
Step 1, choose consistent 3 week Itanlian rye 48 strains in age of growth, implant in 6 identical flowerpots with every basin 8 strains, mixed-matrix is housed in the flowerpot, mixed-matrix is comprised of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, it is 25 ℃ in temperature, illumination every day is under 8 hours conditions, cultivated for 7 weeks, cut off the blade of Itanlian rye, all Itanlian ryes all stay identical stubble high, and stubble is high to be 5cm, then 6 basin Itanlian ryes are divided into 2 groups at random, every group of 3 basins, namely test group a and test group b are for subsequent use;
Step 2, get the Itanlian rye among the test group a, the root water of Itanlian rye in every basin is washed out, after the root of Itanlian rye is separated with stubble, obtain Itanlian rye root and Itanlian rye stubble, Itanlian rye root and Itanlian rye stubble sent in 75 ℃ the drying baker, oven dry, obtain every basin Itanlian rye root oven dry thing and Itanlian rye stubble oven dry thing, detect biomass and carbon content in every basin Itanlian rye root and the Itanlian rye stubble oven dry thing, after calculate the average carbon reserves in the Itanlian rye stubble before the average carbon reserves of Itanlian rye root before the defoliation and the defoliation, for subsequent use;
Step 3, be under 25 ℃ of conditions with the Itanlian rye among the test group b in temperature, carry out lucifuge and cultivate, every 6 days, cut newborn Itanlian rye blade in the step 1 institute high position of toothing, cut 3 blades after Itanlian rye stopped growth; The Itanlian rye blade of at every turn cutting sent in 75 ℃ the drying baker, oven dry, detect biomass and carbon content in the oven dry thing, obtain the carbon reserves of every basin Itanlian rye regeneration blade behind each defoliation, calculate every basin regeneration blade total carbon reserves, obtain at last the average total carbon reserves of Itanlian rye regeneration blade, for subsequent use;
Step 4, utilize the average carbon reserves C of Itanlian rye root before the defoliation
r, the average carbon reserves C before the defoliation in the Itanlian rye stubble
sAverage total carbon reserves C with Itanlian rye regeneration blade
n, according to formula RI=
Calculate the relative maximum RI of stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves;
The result shows, RI is 25.23%, namely before jointing about 20 days the time, leaves that stubble is high be the Itanlian rye of 5cm, and the carbohydrates of storing in its body that leaf regeneration utilizes the relatively amount of maximum is 25.23%;
In technique scheme, used mixed-matrix is lighter in carrying, separates with root through washing easily, has simultaneously good water-retaining property and gas penetration potential, and it is nutritious, is conducive to the growth of Itanlian rye root system;
In technique scheme, the carbohydrate species of storing in the Itanlian rye body is very many, and glucose, sucrose, levulan and starch etc. are arranged, and these carbohydrates all contain carbon, by measuring carbon, can reflect the relative quantity of carbohydrates;
In the technique scheme, adopt the purpose of lucifuge cultivation in order to make the carbohydrates of storing in its body of ryegrass leaves regeneration, because photosynthesis stops fully after the lucifuge, its regrowth can only utilize the carbohydrates of storing in its body;
In the technique scheme, by the repeatedly defoliation under the lucifuge condition, thereby allow Itanlian rye stop growing, so just can realize the utilization of the limit degree of the carbohydrates stored before leaf regeneration is to its defoliation.The carbon of storing before the defoliation that utilizes by defoliation rear blade regeneration, the front ratio of storing carbon of the leaf that accounts for, just can reflect the storage carbohydrate that much ratios are arranged on earth be used directly to the to regenerate growth of blade, so just obtain the relative maximum of stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves.
Claims (1)
1. measure the method for stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves, it is characterized in that: its measuring method is:
Step 1, choose consistent 3 week Itanlian rye 36-48 strains in age of growth, implant in 6 identical flowerpots with every basin 6-8 strains, mixed-matrix is housed in the flowerpot, mixed-matrix is comprised of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, be 17-25 ℃ in temperature, illumination every day is under 6-8 hours conditions, cultivated for 7 weeks, cut off the blade of Itanlian rye, all Itanlian ryes all stay identical stubble high, and stubble is high to be 1-5cm, then 6 basin Itanlian ryes are divided into 2 groups at random, every group of 3 basins, namely test group a and test group b are for subsequent use;
Step 2, get the Itanlian rye among the test group a, the root water of Itanlian rye in every basin is washed out, after the root of Itanlian rye is separated with stubble, obtain Itanlian rye root and Itanlian rye stubble, Itanlian rye root and Itanlian rye stubble sent in 75 ℃ the drying baker, oven dry, obtain every basin Itanlian rye root oven dry thing and Itanlian rye stubble oven dry thing, detect biomass and carbon content in every basin Itanlian rye root and the Itanlian rye stubble oven dry thing, after calculate the average carbon reserves in the Itanlian rye stubble before the average carbon reserves of Itanlian rye root before the defoliation and the defoliation, for subsequent use;
Step 3, be under 20-25 ℃ of conditions with the Itanlian rye among the test group b in temperature, carrying out lucifuge cultivates, every 6 days, cut newborn Itanlian rye blade in the step 1 institute high position of toothing, until all Itanlian ryes among the test group b are ended when stopping growing; The Itanlian rye blade of at every turn cutting sent in 75 ℃ the drying baker, oven dry, detect biomass and carbon content in the oven dry thing, obtain the carbon reserves of every basin Itanlian rye regeneration blade behind each defoliation, calculate every basin regeneration blade total carbon reserves, obtain at last the average total carbon reserves of Itanlian rye regeneration blade, for subsequent use;
Step 4, utilize the average carbon reserves C of Itanlian rye root before the defoliation
r, the average carbon reserves C before the defoliation in the Itanlian rye stubble
sAverage total carbon reserves C with Itanlian rye regeneration blade
n, according to formula RI=
Calculate the relative maximum RI of stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves.
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| CN 201110420458 CN102565313B (en) | 2011-12-15 | 2011-12-15 | Method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves |
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| CN102919065B (en) * | 2012-11-17 | 2014-03-12 | 河南科技大学 | Method for determining regeneration capacity of italian ryegrasses with different stubble heights |
| CN102919066B (en) * | 2012-11-17 | 2014-05-28 | 河南科技大学 | Method for determining regeneration capacity of italian ryegrasses with different stubble heights by ribosylzeatin |
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