CN102914569A - Electrochemistry biochip incubating box - Google Patents
Electrochemistry biochip incubating box Download PDFInfo
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- CN102914569A CN102914569A CN2012101386503A CN201210138650A CN102914569A CN 102914569 A CN102914569 A CN 102914569A CN 2012101386503 A CN2012101386503 A CN 2012101386503A CN 201210138650 A CN201210138650 A CN 201210138650A CN 102914569 A CN102914569 A CN 102914569A
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- box
- upper cover
- chip
- electrochemica biological
- hatched
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Abstract
The invention discloses an electrochemistry biochip incubating box. The electrochemistry biochip incubating box comprises a bottom box, an upper cover, a chip bracket and a catching groove, wherein, when the upper cover and the bottom box are combined, the catching groove can be clamped on the upper part of the upper cover to form a closed chamber. The bottom box and the upper cover are respectively provided with a biological shielding gas inlet and a biological shielding gas outlet, particularly, a specific water absorption material is arranged on the bottom box. According to the incubating box, biological shielding gas and a closed environment with certain humidity are provided for a electrochemistry biochip, so that bioactive molecules modified on the surface of the electrochemistry biochip are prevented from losing biological activity caused by oxidation when being contacted with air or the volatilizing of rare trace samples due to a humidity effect in the incubating process can be reduced.
Description
Technical field
The present invention relates to the biochip technology field, particularly can avoid the electrochemica biological chip of outside contamination to hatch box for the electrochemica biological chip provides biological protection gas and certain humidity environment.
Background technology
Have that volume is little, lightweight, cost low and the advantages such as stable performance, high conformity based on the model electrochemical biochip on MEMS (MEMS) and bioelectrochemistry basis, and laboratory and market all do not have corresponding report of hatching box at present.The bioactive molecule of modifying due to the electrochemica biological chip surface is in its biochemical reaction process, can because after ingress of air, oxidation occurs, cause some molecules to lose biological action, or because being subjected to humidity effect to cause precious micro-example to volatilize, cause the opposite sex of late detection signal not high, even can't detect.Therefore, a kind of have leakproofness and can provide the electrochemica biological chip of biological protection gas to hatch box, is of great significance for improving electrochemica biological chip detection specificity and practicality tool.
Summary of the invention
The objective of the invention is for the electrochemica biological chip provides a kind of box of hatching, it can avoid outside contamination for the electrochemica biological chip provides biological protection gas and certain humidity environment, improves the specificity of later stage electrochemica biological chip detection.
For achieving the above object, the present invention takes following technical scheme: a kind of electrochemica biological chip is hatched box, box, a upper cover, a chip carriage and a catching groove at the bottom of it comprises one.
Above-mentioned electrochemica biological chip is hatched in box, and a biological protection gas air intake opening is contained on box right side, the described end, is used for inputting inert gas, and gas port seals with a soft gas cap; The inwall surrounding is equipped with the specificity absorbent material, provides a moistening environment of hatching for hatching box, avoids the volatilization of chip surface agents useful for same; An o-ring groove is contained in box top, the end and upper cover junction, is placed with the O RunddichtringO in this groove.
Above-mentioned electrochemica biological chip is hatched in box, and described upper cover and end box are by embedded rotation shaft structure sealing, and the phenomenon of avoiding hatching box one side leakage gas occurs; Upper cover contains an exhausr port, is used for getting rid of the foreign gas of hatching the box the inside, and gas port seals with a soft gas cap.
Above-mentioned electrochemica biological chip is hatched in box, described chip carriage contains a rectangular recess, groove is used for fixing and places the electrochemica biological chip, there is the semicircle breach of two symmetries its centre, be used for conveniently taking chip, respectively there is a hand holding handle on the carriage both sides, are conducive to the chip carriage and take in end box conveniently.
Above-mentioned electrochemica biological chip is hatched in box, described catching groove is fixed on box bottom, the end by embedded rotating shaft, when upper lid and bottom box in conjunction with the time, catching groove can be stuck in upper cover top, provide enough pressure to O type circle, guarantee in the air intake opening air inlet or sealing that after bleeding, the electrochemica biological chip is hatched box.
Above-mentioned electrochemica biological chip is hatched in box, and the material of box of the described end, upper cover, chip carriage can be selected from polyvinyl fluoride, ABS, polycarbonate, polystyrene, tygon, organic glass or polypropylene; Described catching groove material can be selected from stainless steel, magnalium or titanium alloy etc.; Described sealing gland cap and O RunddichtringO material can be selected from silicon rubber.
The present invention takes above project organization to have the following advantages: the upper lid and bottom box in the present invention can form an airtight chamber; upper cover and end box contain respectively a biological protection gas to be imported and exported; the box-packed specificity absorbent material that has in the end particularly; this invention can provide for the electrochemica biological chip closed environment of biological protection gas and certain humidity; avoid modifying bioactive molecule in the electrochemica biological chip surface in its biochemistry is hatched process, because ingress of air generation oxidation loses biologically active or because being subjected to humidity effect to cause precious micro-example to volatilize.
Description of drawings
Fig. 1 be inside of the present invention be placed with the electrochemica biological chip hatch the box stereographic map: closed (figure is left), uncap (figure is right)
Fig. 2 is the stereographic map that electrochemica biological chip of the present invention is hatched the box exploded view.
Fig. 3 is that electrochemica biological chip of the present invention is hatched the box front view.
Fig. 4 is that electrochemica biological chip of the present invention is hatched the box left view.
Fig. 5 is that electrochemica biological chip of the present invention is hatched the box vertical view.
1. sealing gland caps in figure, 2. exhausr port, 3. biochemical reaction groove, 4. electrochemica biological chip, 5. chip carriage, 6. rectangular recess, 7. hand holding handle, 8. semicircle breach, 9.O RunddichtringO, 10. water-absorbing material, 11. rotary axis grooves, 12. rotating shafts, 13. the sealing gland cap, 14. air intake openings, 15.O RunddichtringO groove, 16. upper cover, 17. catching grooves, 18. end boxes.
Specific embodiments:
Fig. 1 to Fig. 5 is that electrochemica biological chip provided by the present invention is hatched box, and it comprises box 18 at the bottom of a upper cover 16,, a chip carriage 5 and a catching groove 17; Wherein upper cover 16 comprises a gentle sealing cap 1 of exhausr port 2; Chip carriage 5 comprises a rectangular recess 6, and the semicircle breach 8 of two symmetries is arranged in the middle of groove, and respectively there is a hand holding handle 7 on the both sides of carriage, and biochemical reaction groove 3 correspondences are placed on the surface of electrochemica biological chip 4, and during use, integral body is placed on rectangular recess 6; End box 18 contains an air intake opening 14, by 13 sealings of sealing gland cap, end box 18 tops and upper cover 16 junctions have a rectangle O RunddichtringO groove 15 to be used for placing O RunddichtringO 9, surrounding is equipped with water-absorbing material 10, end box 18 by rotation axis 12 and rotary axis groove 11, consists of insert seal structure as shown in Figure 5 with upper cover 16.Catching groove 17 is fixed on end box 18 bottoms by embedded rotating shaft, when upper cover 3 and end box 18 in conjunction with the time, catching groove 17 can be stuck in upper cover 16 tops, consists of the hermetically-sealed construction as Fig. 4.
Above-mentioned upper cover 16, end box 18, carriage 5 processing technology used are injection molding, material therefor can be selected from the plastics such as polyvinyl fluoride, ABS, polycarbonate, polystyrene, tygon, organic glass or polypropylene, catching groove 17 processing technologys are precision optical machinery processing, and material can be selected from stainless steel, magnalium or titanium alloy etc.O RunddichtringO 9, sealing gland cap 1,13 processing technology are injection molding, and material can be selected from silicon rubber.Water-absorbing material 10 can be selected from cellulose nitrate, sponge or foam etc., and the electrochemica biological chip is hatched before box uses, and water be joined absorbent material 10 surfaces.
Specific embodiments of the invention are as follows:
Water-absorbing material 10 in end box 18 surroundings adds the 5ml distilled water; Biochemical reaction groove 3 is added the surface that is placed on electrochemica biological chip 4, put into rectangular recess 6 the insides of chip carriage 5, then by hand holding handle 7, integral body is put into end box 18 inside, shown in Fig. 1 (uncap, figure is right); After biochemical reagents being added the circular reaction tank of biochemical reaction groove 3, rapid closing upper cover 16 is buckled draw-in groove 17, and at this moment upper cover 16 under the acting force of catching groove 17, forms an airtight cavity by O RunddichtringO 9 with end box 18; Remove sealing gland cap 1 and 13, biological protection gas (mostly being nitrogen) is passed into the 2-3min of airtight chamber by end box air intake opening 14, air in chamber is drained by exhausr port 2, rapidly sealing gland cap 1 and 13 is covered respectively on air intake opening and exhausr port, a biological protection compression ring border is provided, shown in Fig. 1 (closure, figure is left); The electrochemica biological chip is hatched box put into incubator and hatch according to the required temperature and time of biochemical reagents, the absorbent material 10 in end this moment box 18 will provide certain humidity for hatching box airtight chamber, avoid causing because Temperature of Warm Case is too high the reagent volatilization; Hatch and open upper cover 16 after completing, by hand holding handle 7, chip carriage 5 is taken out from hatch box, then symmetrical semicircle breach 8 in the middle of the chip carriage 5, take out the electrochemica biological chip 4 that is coated with biochemical reaction groove 3, carries out subsequent detection.
Claims (8)
1. an electrochemica biological chip is hatched box, it is characterized in that: box, a upper cover, a chip carriage and a catching groove at the bottom of containing one.
2. electrochemica biological chip according to claim 1 is hatched box, it is characterized in that: a biological protection gas air intake opening is contained on box right side, the described end, and the inwall surrounding is equipped with the specificity absorbent material; An O RunddichtringO groove is contained in box top, the end and upper cover junction, is placed with the O RunddichtringO in this groove.
3. electrochemica biological chip according to claim 1 is hatched box, it is characterized in that: described upper cover and end box avoid the phenomenon of a side leakage gas to occur by embedded rotation shaft structure sealing; Upper cover also contains an exhausr port, is used for getting rid of the foreign gas of the inside.
4. according to claim 2,3 described electrochemica biological chips hatch box, it is characterized in that: the gas port of described upper cover and end box all contains a sealing gland cap, is used for sealing gas port.
5. electrochemica biological chip according to claim 1 is hatched box, it is characterized in that: described chip carriage contains a rectangular recess, the semicircle breach that two symmetries are arranged in the middle of groove, be used for conveniently taking chip, respectively there is a hand holding handle on the carriage both sides, are conducive to the chip carriage and take in end box conveniently.
6. electrochemica biological chip according to claim 1 is hatched box, it is characterized in that: described catching groove is crossed embedded rotating shaft and is fixed on box bottom, the end, when upper lid and bottom box in conjunction with the time, catching groove can be stuck in upper cover top, provide enough pressure to O type circle, form an airtight chamber.
7. according to claim 1,2,3,4,5 described electrochemica biological chips hatch box, it is characterized in that: the material of box of the described end, upper cover, chip carriage is polyvinyl fluoride, ABS, polycarbonate, polystyrene, tygon or polypropylene; Water-absorbing material is cellulose nitrate, sponge or foam; The material of O RunddichtringO and sealing gland cap is silicon rubber.
8. electrochemica biological chip according to claim 6 is hatched box, it is characterized in that: described catching groove material is stainless steel, magnalium, titanium alloy or nickel-chrome.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101386503A CN102914569A (en) | 2012-05-07 | 2012-05-07 | Electrochemistry biochip incubating box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101386503A CN102914569A (en) | 2012-05-07 | 2012-05-07 | Electrochemistry biochip incubating box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102914569A true CN102914569A (en) | 2013-02-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101386503A Pending CN102914569A (en) | 2012-05-07 | 2012-05-07 | Electrochemistry biochip incubating box |
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| Country | Link |
|---|---|
| CN (1) | CN102914569A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1948479A (en) * | 2006-11-09 | 2007-04-18 | 博奥生物有限公司 | Biochip hybridizing box |
| CN200964254Y (en) * | 2006-09-19 | 2007-10-24 | 富创得科技股份有限公司 | Nitrogen cabinet with identification and aeration device |
| CN201569655U (en) * | 2009-12-17 | 2010-09-01 | 东莞市兴仁保健食品有限公司 | Immunoblot antibody incubation box |
| CN201945591U (en) * | 2010-12-15 | 2011-08-24 | 广州金域医学检验中心有限公司 | Temperature displaying incubation box |
-
2012
- 2012-05-07 CN CN2012101386503A patent/CN102914569A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200964254Y (en) * | 2006-09-19 | 2007-10-24 | 富创得科技股份有限公司 | Nitrogen cabinet with identification and aeration device |
| CN1948479A (en) * | 2006-11-09 | 2007-04-18 | 博奥生物有限公司 | Biochip hybridizing box |
| CN201569655U (en) * | 2009-12-17 | 2010-09-01 | 东莞市兴仁保健食品有限公司 | Immunoblot antibody incubation box |
| CN201945591U (en) * | 2010-12-15 | 2011-08-24 | 广州金域医学检验中心有限公司 | Temperature displaying incubation box |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
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Application publication date: 20130206 |