CN102898225A - Composite manure water preparation and application thereof - Google Patents

Composite manure water preparation and application thereof Download PDF

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Publication number
CN102898225A
CN102898225A CN201210342073XA CN201210342073A CN102898225A CN 102898225 A CN102898225 A CN 102898225A CN 201210342073X A CN201210342073X A CN 201210342073XA CN 201210342073 A CN201210342073 A CN 201210342073A CN 102898225 A CN102898225 A CN 102898225A
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China
Prior art keywords
rich water
water preparation
compound formulation
manure
sulfate
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CN201210342073XA
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CN102898225B (en
Inventor
王纪荣
王纪卫
王纪军
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ZHEJIANG TIANDIRUN PEARL CO Ltd
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ZHEJIANG TIANDIRUN PEARL CO Ltd
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Priority to CN201210342073.XA priority Critical patent/CN102898225B/en
Publication of CN102898225A publication Critical patent/CN102898225A/en
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Publication of CN102898225B publication Critical patent/CN102898225B/en
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Abstract

The invention relates to a composite manure water preparation which comprises the following substances by weight percent: 80 to 92 % of decomposed chicken manure, 3 to 10 % of calcium superphosphate, 1 to 5 % of potassium dihydrogen phosphate, 0.5 to 5 % of calcium carbonate, 0.02 to 0.35 % of zinc sulfate, 0.04 to 0.70 % of manganese sulphate, 0.02 to 0.2 % of manganese chloride, 0.1 to 0.6 % of copper sulfate, 0.1 to 0.7 % of ferrous sulfate heptahydrate, 0.02 to 0.2 % ammonium molybdate, 0.02 to 0.35% of the boron ions and 0.5 to 1 % of cyclotella. In addition, the composite manure water preparation provided by the invention is added with bacterial liquid of Bacillus subtilis, lactic acid bacteria and bifidobacteria. According to the invention, by using the fermented duck manure as main raw materials, together with N, P, K, trace elements, etc. necessary for phytoplankton and zooplankton, the prepared organic manure water preparation is sufficient in organic nutrients and trace element nutrients, and reasonable in compatibility, and can quickly supplement small molecular organic debris needed by pearl mussel, and N, P, K and a variety of trace elements needed by the phytoplankton and the zooplankton.

Description

A kind of rich water compound formulation and application thereof
Technical field
The invention belongs to the agriculture production product technique field, be specifically related to a kind of rich water compound formulation and application thereof, be i.e. a kind of rich water compound formulation of suitable pearl culturing clam cultivation waters use.
Background technology
At present, the rich water product of culture fishery is a lot, but mostly configures for fishery products such as fishes and shrimps.And the cultivation of pearl freshwater mussel requires with general breed variety difference, and present rich water product does not satisfy the pearl freshwater mussel to the demand of the basic nutritive elements such as nitrogen, phosphorus, potassium, calcium.And, also have a lot of mineral elements and trace element also very important at the foster freshwater mussel fishing waters of growing cultured pearls, growth and being formed with directly and indirectly of pearl of pearl culturing clam affected.For example, nitrogen, phosphorus, potassium are the necessary elements of phytoplankton growth and breeding, and plant plankton is the primary productivity of water body, and plankton and organic debris are the basic substances that pearl culturing clam is depended on for existence.Food organism are abundant in water, the nutritional sufficiency of pearl culturing clam, and pearl also just looks soon, and quality is high.It is different that this and general aquaculture need to be thrown in bait.Consider that calcium (existing with calcium carbonate form) is the major ingredient of pearl culturing clam shell and pearl, therefore, the calcium contents that the aquaculture water of pearl culturing clam requires is higher than ortho-water product demand.Simultaneously, all require certain content for copper, zinc, manganese, iron etc.Therefore exploitation has very great meaning for the rich water preparation of pearl culturing clam aquaculture water special use.
Summary of the invention
The purpose of this invention is to provide the rich water compound formulation that use in a kind of suitable pearl culturing clam cultivation waters, have that calcic is high, nutritious, fertilizer efficiency is both lasting rapidly, can go out plant plankton and zooplankton by high effect culture.
Rich water compound formulation of the present invention includes the material of following weight percent: the duck excrement 80~92% that becomes thoroughly decomposed, calcium superphosphate 3~10%, potassium primary phosphate 1~5%, calcium carbonate 0.5~5%, zinc sulfate 0.02~0.35%, manganous sulfate 0.04~0.70%, Manganous chloride tetrahydrate 0.02~0.2%, copper sulfate 0.1~0.6%, iron vitriol 0.1~0.7%, ammonium molybdate 0.02~0.2%, boron ion 0.02~0.35%, little ring algae 0.5~1%.
Rich water compound formulation of the present invention also is added with the bacterium liquid of genus bacillus, milk-acid bacteria and bifidus bacillus, makes genus bacillus, milk-acid bacteria and the bifidus bacillus quantity in compound formulation be respectively 10 6~10 8CFU/kg.
The present invention utilizes duck excrement after the fermentation as main raw material, cooperates plant plankton and the necessary N of zooplankton, P, K and trace element etc., the fertilizer water preparation of making, and organic nutrient and micronutrient are sufficient, cooperate rationally.Use the advantage of rich water compound formulation of the present invention as follows:
(1) the present invention can replenish the required small molecules organic debris of pearl culturing clam and plankton needs N, P, K and various trace elements fast.
(2) part material of the present invention is taken from and is supported the waste material that duck already produces, and turns waste into wealth, and has avoided a large amount of wastings of resources, and preparation is simple and easy to use.
(3) the present invention can be used for green, organic foodstuff production.
Embodiment
The preparation method of duck excrement of becoming thoroughly decomposed adopts traditional method of naturally becoming thoroughly decomposed, and becomes thoroughly decomposed after the duck excrement is piled up, and stirs therebetween, and to duck excrement odorless, the brown that darkens to black has just been finished the preparation of the duck excrement that becomes thoroughly decomposed.
In order specifically to increase the content of little ring algae, be added with the live body of a small amount of little ring algae in the rich water agent of the present invention as the algae kind, when using, can effectively improve the amount of little ring algae in aquaculture water like this, the bait of abundance is provided for the growth of pearl culturing clam.
In order to strengthen the effect of rich water preparation of the present invention, in the rich water preparation of preparation, also optionally be added into the viable bacteria of genus bacillus, milk-acid bacteria and bifidus bacillus.Wherein the substratum of genus bacillus is composed as follows: 1L distilled water+20g glucose+15g peptone+5g sodium-chlor+0.5g extractum carnis+20g agar;
Milk-acid bacteria substratum (MRS solid medium) is as follows: peptone 10g, extractum carnis 10g, yeast extract paste 5g, K 2HPO 43H 2O 2g, sodium acetate 5g, glucose 20g, tween 80 1g, dibasic ammonium citrate 2gMgSO 47H 2O 0.58g, MnSO 44H 2O 0.25g, adding distil water 1000mL transfers PH6.2-6.4 at 121 ℃, and the 15min sterilization is for subsequent use;
Medium of Bifidobacterium is selected the BS substratum, can buy the Bioisystech Co., Ltd from Qingdao Hai Bo.
After the substratum of genus bacillus, milk-acid bacteria, bifidus bacillus prepared, respectively bacterial classification is inoculated in the substratum according to 2% inoculum size, after the access, behind 35 ℃ of incubated overnight 24~48h, or measuring OD600 value is to stop cultivation at 0.5 o'clock.
The present invention is described further and verification experimental verification below in conjunction with embodiment.
Embodiment 1
The rich water preparation of the present embodiment, raw material by following weight percent is made: fermentation duck excrement 91%, calcium superphosphate 3%, potassium primary phosphate 2.3%, calcium carbonate 0.5%, zinc sulfate 0.2%, manganous sulfate 0.3%, Manganous chloride tetrahydrate 0.2%, copper sulfate 0.5%, iron vitriol 0.5%, ammonium molybdate 0.2%, boric acid 0.3%.The said products is added the stirrer stirring and evenly mixing.Then add the algae liquid of little ring algae, air-dry, making the weight percent of little ring algae in the rich water agent is 1%.
Little ring algae is bought from hydrobiont institute of Wuhan Chinese Academy of Sciences algae kind storehouse, is numbered FACHB-1030, and substratum is selected the D1 substratum.
Embodiment 2
The present embodiment and embodiment 1 difference are that the mass percent of raw material is different, and its preparation method is identical with embodiment 1.
Fermentation duck excrement 87.5%, calcium superphosphate 5%, potassium primary phosphate 3.8%, calcium carbonate 0.5%, zinc sulfate 0.3%, manganous sulfate 0.6%, Manganous chloride tetrahydrate 0.1%, copper sulfate 0.6%, iron vitriol 0.7%, ammonium molybdate 0.2%, boric acid 0.2%, little ring algae 0.5%.
Embodiment 3
The present embodiment and embodiment 1 difference are that the mass percent of raw material is different, and its preparation method is identical with embodiment 1.
Fermentation duck excrement 87.5%, calcium superphosphate 5%, potassium primary phosphate 3.8%, calcium carbonate 0.5%, zinc sulfate 0.3%, manganous sulfate 0.6%, Manganous chloride tetrahydrate 0.1%, copper sulfate 0.6%, iron vitriol 0.7%, ammonium molybdate 0.2%, boric acid 0.2%, little ring algae 0.5%.
After preparing above-mentioned rich water preparation, the cultivation bacterium liquid adding of genus bacillus, milk-acid bacteria, bifidus bacillus is stirred, make the quantity of three kinds of bacterium in compound formulation be respectively 10 6~10 8Then CFU/kg makes the rich water agent of the present embodiment after the drying.Wherein genus bacillus can be selected available from Chinese agriculture microbial strains preservation center, is numbered the bacterial strain of 1511C0001ACCC02728; Bifidus bacillus is numbered 1511C0001ACCC11054 available from Chinese agriculture microbial strains preservation center; Milk-acid bacteria also available from Chinese agriculture microbial strains preservation center, is numbered 1511C0001ACCC10637.
Because the viable bacteria of having added probiotics in the rich water agent, before the rich water agent was used, bacterium also can again carry out biological degradation to fermentation duck excrement and process; Therefore, compare than the rich water agent of not adding bacterium, have extra beneficial effect in the time of use.
The using method of rich water compound formulation of the present invention:
With preparation water 1:10 dilution, the culture pond of splashing after mixing, 1 meter pond usage quantity of every mu of depth of water is 1-3Kg.Concrete usage quantity is decided on water colour, and the season of growth of the 5-10 month, every two weeks once.
The experimental study of rich water compound formulation:
Water test is trained in pearl freshwater mussel cultivation waters in the world, Shaoxing, Zhejiang profit company, 22 ℃ of water temperatures, 1 meter of the depth of water, the water surface are 5 mu, use the rich water compound formulation 10Kg of embodiment 1, around fetching water in each pond after the same day and 10 days, test reaches 1 part of each water sampling of central authorities, fully mixing is got 1 L mixed sample and is poured vial into, adds 5% formaldehyde solution and fixes, leave standstill 24-48h, concentrated rear at plant plankton counting frame microscopy, counting.The result shows, the planktonic microalgae cell quantity in the water body is 4 times before using, and little ring algae number preponderates, and rolled up the bait of pearl culturing clam, lays a solid foundation for bringing out the high quality pearl.
Adopt embodiment 2,3 rich water agent to carry out identical test, also can reach rich water effect of the present invention, the purpose that provides pearl culturing clam to grow required plant plankton and zooplankton is provided.And owing to having added probiotics among the embodiment 3, nacreous sickness rate is all lower than the preparation of embodiment 1 and 2 in cultivation.

Claims (3)

1. a rich water compound formulation is characterized in that, includes the material of following weight percent: duck excrement 80~92% becomes thoroughly decomposed, calcium superphosphate 3~10%, potassium primary phosphate 1~5%, calcium carbonate 0.5~5%, zinc sulfate 0.02~0.35%, manganous sulfate 0.04~0.70%, Manganous chloride tetrahydrate 0.02~0.2%, copper sulfate 0.1~0.6%, iron vitriol 0.1~0.7%, ammonium molybdate 0.02~0.2%, boron ion 0.02~0.35%, little ring algae 0.5~1%.
2. rich water compound formulation as claimed in claim 1 is characterized in that described rich water compound formulation also is added with genus bacillus, milk-acid bacteria and bifidus bacillus.
3. rich water compound formulation as claimed in claim 2 is characterized in that described genus bacillus, milk-acid bacteria and the bifidus bacillus quantity in compound formulation is respectively 10 6~10 8CFU/kg.
CN201210342073.XA 2012-09-16 2012-09-16 Composite manure water preparation and application thereof Expired - Fee Related CN102898225B (en)

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Application Number Priority Date Filing Date Title
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CN102898225A true CN102898225A (en) 2013-01-30
CN102898225B CN102898225B (en) 2014-06-11

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1410397A (en) * 2002-12-06 2003-04-16 赵鹏 Biological organic and inorganic composite fertilizer and its production method
CN101225007A (en) * 2008-02-03 2008-07-23 武汉市科洋生物工程有限公司 Special biological fish-fertilizer for aquaculture and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1410397A (en) * 2002-12-06 2003-04-16 赵鹏 Biological organic and inorganic composite fertilizer and its production method
CN101225007A (en) * 2008-02-03 2008-07-23 武汉市科洋生物工程有限公司 Special biological fish-fertilizer for aquaculture and preparation method thereof

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