CN102885044A - Chitosan oligosaccharide composition for preventing and controlling rice plant hoppers and use and using method thereof - Google Patents

Chitosan oligosaccharide composition for preventing and controlling rice plant hoppers and use and using method thereof Download PDF

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CN102885044A
CN102885044A CN2011103330692A CN201110333069A CN102885044A CN 102885044 A CN102885044 A CN 102885044A CN 2011103330692 A CN2011103330692 A CN 2011103330692A CN 201110333069 A CN201110333069 A CN 201110333069A CN 102885044 A CN102885044 A CN 102885044A
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chitosan oligosaccharide
composition
rice
pesticide
rice plant
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CN102885044B (en
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张善学
侯华民
陆红霞
陈坚
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Hainan Zhengye Biotechnology Co ltd
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Hainan Zhengye Zhongnong Hi Tech Co Ltd
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Abstract

The invention relates to a chitosan oligosaccharide composition for preventing and controlling rice plant hoppers, and use and a using method of the chitosan oligosaccharide composition, and particularly provides a pesticide composition for preventing and controlling the rice plant hoppers, wherein the pesticide composition comprises chitosan oligosaccharide which accounts for 0.004 to 0.025 percent of the weight of the pesticide composition. The invention also provides the use of the chitosan oligosaccharide-comprising pesticide composition and a method for using the chitosan oligosaccharide-comprising pesticide to prevent and control the rice plant hoppers. By the pesticide composition and the method, the rice plant hoppers can be prevented and controlled, particularly after crops which are infected by the rice plant hoppers are treated by using the chitosan oligosaccharide-comprising pesticide, the rice plant hoppers are prevented and controlled, phytotoxicity is not generated, pesticide residues are not produced, and environment-friendly prevention and control over the rice plant hoppers is realized.

Description

A kind of chitosan oligosaccharide composition and use thereof and method for the control rice fulgorid
Technical field
The invention belongs to agrochemical field, particularly relate to the control composition of rice fulgorid and prevent and treat method, relate to particularly a kind of chitosan oligosaccharide composition and use thereof and method for the control rice fulgorid.
Background technology
Rice fulgorid (comprising brown planthopper, white-backed planthopper and small brown rice planthopper) belongs to Homoptera, and Delphacidae is that China also is one of main migratory pest on the Asian countries paddy rice.Have at a distance migrating property habit, be distributed widely in the torrid zone, subtropics and the Temperate Region in China in Asia, comprise the ground such as the South Asian Subcontinent, Southeast Asia, archipelago, the South Pacific Ocean and Australia.The brown planthopper feeding habits are single, only take rice-cultivating and wild rice as the host.Its hazard approach: main by directly stinging the liquid nutritional material of inhaling in the rice strain phloem, paddy growth is suppressed; The saliva of secretion can form stylophore when taking food, and obstruction fascicular system is unified and hindered the transportation of the interior moisture of rice strain and nutrition, forms " emit and wear " thereby cause the paddy rice dehydration to wilt.In addition, disease, careless shape disease and the tingia disease of stunting of stunting of stunting of also propagate wilting in taking food process has a strong impact on paddy growth, causes rice lodging, make the paddy underproduction or total crop failure, thereby various countries attaches great importance to all to the research of brown property lice.
It is northern that white-backed planthopper is distributed widely in South Asia, Southeast Asia, Japan, Korea, China, archipelago, the South Pacific Ocean and Australia, the report that the white-backed planthopper hazard rice is also arranged in the Egypt in Africa, even in the place that does not have rice cultivation, such as Mongolia, Siberia, Karafuto and other places the record of adopting long wing type adult is arranged also.White-backed planthopper is widely distributed in China, almost spreads all over all rice districts, comprises south China, Central China, North China, northeast and southwestern rice district, and northwest rice district can reach the ground such as the municipal Miquan in Xinjiang.
The white-backed planthopper hazard rice causes rice strain poor growth, minute grain delay, flat grain to increase mainly directly to suck rice strain phloem sap or to lay eggs and the paddy rice of directly causing harm in stem.It is withered to cause the rice strain when serious, is " lice burning " shape, makes rice yield be subject to massive losses.
Prevent and treat at present rice fulgorid chemical pesticide organic phosphates: chlorpyrifos ec, triazophos emulsifiable solution; Nicotinoids: Nitenpyram, imidacloprid wettable powder; New heterocyclic: thiazolone wetting powder and pymetrozine; Amide-type: Rynaxypyr; Biogenic substances: first dimension salt, Avermectin; Carbamate insecticide: meta-tolyl-N-methylcarbamate (MTMC) missible oil, Bassa missible oil, Mobucin.
There is not at present fully agricultural chemicals and the scientific and effective method of preventing and treating that can either environmental protection can effectively prevent and treat again rice fulgorid
The present inventor is by long-term field research, and acquisition can effectively prevent and treat the chitosan oligosaccharide agricultural chemicals of rice fulgorid, finds that chitosan oligosaccharide is effective chemical substance of control rice fulgorid.And by further investigation, obtained the control rice fulgorid chitosan oligosaccharide concentration, application process and use period.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of composition pesticide for the control rice fulgorid, comprise chitosan oligosaccharide in this composition pesticide, the percentage by weight that chitosan oligosaccharide accounts for this composition pesticide is 0.004%-0.025%.Preferably, to account for the percentage by weight of this composition pesticide be 0.005%-0.012% to chitosan oligosaccharide.
Above-mentioned this composition pesticide can prepare liquid preparation or solid pharmaceutical preparation.Liquid preparation can be any liquid preparation of agriculture usefulness, and solid pharmaceutical preparation also is any solid pharmaceutical preparation of agriculture usefulness.Preferably, liquid preparation is aqua, microemulsion, water dispersible granules or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or granule.
The present invention also provides the above-mentioned composition pesticide that comprises chitosan oligosaccharide to be used for the purposes of control rice fulgorid, the percentage by weight that it is characterized in that comprising chitosan oligosaccharide in the composition pesticide of chitosan oligosaccharide is 0.004%-0.025%, preferably, to account for the percentage by weight of composition be 0.005%-0.012% to chitosan oligosaccharide.
The present invention also provides the method for using the composition for preventing and controlling rice fulgorid that comprises chitosan oligosaccharide, it is characterized in that using composition at the sick initial stage of sending out of rice fulgorid, then every time of 5-10 days, preferably every time of 7 days, again use and the identical or different composition of application concentration first 0-3 time, preferably, use composition 2 times, the percentage by weight of chitosan oligosaccharide is 0.004%-0.025% in the wherein said composition, preferably, to account for the percentage by weight of composition be 0.005%-0.012% to chitosan oligosaccharide.
In the said method, described composition can prepare liquid preparation or solid pharmaceutical preparation.Liquid preparation can be any liquid preparation of agriculture usefulness, and solid pharmaceutical preparation also is any solid pharmaceutical preparation of agriculture usefulness.Preferably, liquid preparation is aqua, microemulsion, water dispersible granules or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or granule.
In the said method, any method of effectively commonly using the when method of using refers to the applications of pesticide includes but not limited to powder injection process, spray-on process, fumigating system, soil treatment method or smog method etc.
Wherein powder injection process refers to that the wind-force that utilizes duster to produce is sprinkling upon the agricultural chemicals of pulvis the surface of crop or controlling object equably; Spray-on process refers to be made into liquid with a certain amount of agricultural chemicals and an amount of water, with the spraying apparatus medicine liquid spray is become droplet, is evenly distributed in crop or controlling object surface; Fumigating system refers to adopt fumigant or volatile medicament, makes its volatilization become the effectively gas of prevention and elimination of disease and pests; The soil treatment method refers to take spray, dusts, spreads the methods such as pesticide-clay mixture, and agricultural chemicals is imposed in ground or the certain soil layer equably; The smog method refers to utilize special-purpose facility that the oily pesticide dispersion is become smoke condition, to reach the method for Pesticidal and sterilizing weeding.
In the said method, the sick initial stage of sending out of rice fulgorid refers to that rice fulgorid is sick and sends out firm the incipient stage, ordinary circumstance, and this stage can be judged by range estimation to those skilled in the art, when the rice fulgorid damage to crops is namely arranged, the stage that particularly has the rice fulgorid insect population to occur.Particularly, can judge by the mode of quantitative detection also that be the sick initial stage of sending out of rice fulgorid the period below 3% that the plant of namely rice fulgorid insect population appearance accounts for total plant quantity.
Among the present invention, chitosan oligosaccharide is that the degree of polymerization that Glucosamine is formed by connecting by Isosorbide-5-Nitrae-glycosidic bond is the mixture of the high molecular polymer of 2-30, and mean molecule quantity is 1000-3000 dalton.Chitosan oligosaccharide can prepare by any means, and such as following method: deacetylation is the mixture of the chitosan oligomer of 2-30 at the shitosan more than 70% by the degree of polymerization that the methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation method prepare.Perhaps, be the mixture of the chitosan oligomer of 2-30 by from fungal cell wall, extracting the degree of polymerization that obtains chitin and prepare by methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods.Perhaps, by being the mixture of the polymer of 2-30 by Glucosamine by the degree of polymerization that Isosorbide-5-Nitrae-the glycosidic bond chemosynthesis is polymerized.Chitosan oligosaccharide is the mixture of the high molecular polymer of different polymer at present, that is to say in the chitosan oligosaccharide to be not the oligosaccharides form of the single degree of polymerization, but the chitosan oligosaccharide monose of every kind of degree of polymerization may exist all.So no matter by which kind of method prepare; the commercially available prod that perhaps prepares by above-mentioned preparation method; as long as it is that Glucosamine passes through 1; the degree of polymerization that the 4-glycosidic bond is formed by connecting is that 2-30, mean molecule quantity are that the mixture of the daltonian high molecular polymer of 1000-3000 is exactly chitosan oligosaccharide of the present invention; it can contain acetyl group; but the sugar unit of bonding acetyl group is no more than 40% amount, preferably is no more than 30% amount, especially preferably is no more than 20% amount.Commercially available chitosan oligosaccharide all is the chitosan oligosaccharide product that above-mentioned chitosan oligosaccharide term is set forth basically at present.
The main paddy rice of chitosan oligosaccharide composition for preventing and controlling of the present invention comprises the rice fulgorid on rice-cultivating and the wild paddy rice.
Beneficial effect of the present invention:
The present invention has found to be used for the composition pesticide that comprises chitosan oligosaccharide of control rice fulgorid, this composition pesticide can be prevented and treated rice fulgorid, particularly found the special effect of chitosan oligosaccharide when the control rice fulgorid of certain concentration, solve the major issue of control rice fulgorid, especially use the composition pesticide that comprises chitosan oligosaccharide after crop is caught an illness in processing, not only prevent and treat rice fulgorid, and can be to not doing the deposits yields poisoning, do not have residue of pesticide, realized the control of the rice fulgorid of environment protection-type.The special contribution of the present invention also is to have found to use good chance and the concentration of chitosan oligosaccharide control rice fulgorid by research, has greatly improved the effect of chitosan oligosaccharide control rice fulgorid.
Embodiment
In order to understand the present invention, the below further specifies the present invention with embodiment, but does not limit the present invention.
Embodiment 1:
5% chitosan oligosaccharide solution with water is diluted to respectively corresponding application concentration, concentration is calculated according to weight percent concentration, calculate with ppm (1,000,000/) concentration in outcome record, what 50ppm represented in the outcome record below is that weight percent concentration is 0.005%.Clear water is processed in contrast.Solution after the dilution is in the paddy rice of rice fulgorid their early stage according to the demand treatment by stages, and be 7 days blanking time that sprays processing.Each processing is observed and is detected by 3 experimental field (100 square metres), adds up respectively the PD situation of the rice fulgorid of every experimental field.Be that rice fulgorid nibbles and bites the plant quantity that paddy rice has an insect population and account for 3 ± 0.25% of the total plant number of paddy rice the opportunity of using chitosan oligosaccharide solution-treated rice fulgorid.The rice fulgorid control efficiency has the ratio situation of change statistics of rice fulgorid insect population quantity by inspection.When adding up, the insect population number of control efficiency=(the insect population number of the insect population number of blank-processing ensuing crop)/blank * 100% carries out assembly average according to every experimental field, for example plant is counted the X strain in 100 square metres the experimental field, calculate the average population number of X strain, comprise and processing and blank, the calculating that is not always the case is calculated average control efficiency with this.
Concrete disposition and effect are as follows:
The table 1 rice fulgorid insect pest initial stage is used chitosan oligosaccharide solution to the control efficiency of rice fulgorid
Figure BDA0000102986210000041
Show that by above-mentioned experiment no matter being that the single treatment rice fulgorid is sick sends out the paddy rice at initial stage or process continuously the sick paddy rice that sends out the initial stage of rice fulgorid, all rice fulgorid has been produced good control efficiency, can control the PD of rice fulgorid, until reach the control rice fulgorid.

Claims (3)

1. a composition pesticide that is used for the control rice fulgorid comprises chitosan oligosaccharide in this composition pesticide, and the percentage by weight that chitosan oligosaccharide accounts for this composition pesticide is 0.004%-0.025%.Preferably, to account for the percentage by weight of this composition pesticide be 0.005%-0.012% to chitosan oligosaccharide.
2. the composition pesticide that comprises chitosan oligosaccharide is used for the purposes of control rice fulgorid, the percentage by weight that it is characterized in that comprising chitosan oligosaccharide in the composition pesticide of chitosan oligosaccharide is 0.004%-0.025%, preferably, to account for the percentage by weight of composition be 0.005%-0.012% to chitosan oligosaccharide.
3. use the method for the composition for preventing and controlling rice fulgorid that comprises chitosan oligosaccharide, it is characterized in that using composition at the sick initial stage of sending out of rice fulgorid, then every time of 5-10 days, preferably every 7 days time, again use and the identical or different composition of application concentration first 0-3 time, preferably, use composition 2 times, the percentage by weight of chitosan oligosaccharide is 0.004%-0.025% in the wherein said composition, and preferred, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.005%-0.012%.
CN201110333069.2A 2011-10-28 2011-10-28 Chitosan oligosaccharide composition for preventing and controlling rice plant hoppers and use and using method thereof Active CN102885044B (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003057233A1 (en) * 2002-01-10 2003-07-17 Rnl Life Science Ltd. Composition for preventing or treating mastitis of dairy cattle
CN1520723A (en) * 2003-01-30 2004-08-18 中国科学院上海植物生理研究所 Chitosan oligosaccharide containging pesticide composition and its application
CN101120673A (en) * 2007-09-19 2008-02-13 雷庆峰 Nontoxic pesticide and preparing method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003057233A1 (en) * 2002-01-10 2003-07-17 Rnl Life Science Ltd. Composition for preventing or treating mastitis of dairy cattle
CN1520723A (en) * 2003-01-30 2004-08-18 中国科学院上海植物生理研究所 Chitosan oligosaccharide containging pesticide composition and its application
CN101120673A (en) * 2007-09-19 2008-02-13 雷庆峰 Nontoxic pesticide and preparing method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
刘晓宇 等: "壳寡糖对植物病原真菌的抑制作用", 《安徽农业科学》 *

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