CN102879387B - Reagent plate for measuring bacteria, image information acquisition device, and measurement system - Google Patents
Reagent plate for measuring bacteria, image information acquisition device, and measurement system Download PDFInfo
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- CN102879387B CN102879387B CN201110451102.1A CN201110451102A CN102879387B CN 102879387 B CN102879387 B CN 102879387B CN 201110451102 A CN201110451102 A CN 201110451102A CN 102879387 B CN102879387 B CN 102879387B
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Abstract
The invention discloses a principle of utilizing the growth of bacteria in a constant temperature incubator to generate color and turbidity changes, wherein the color and turbidity changes are generated in the growth process of the bacteria, an LED matrix surface light source uniformly irradiates through the bottom of a concave spherical surface of each cavity on a reagent plate, a camera collects reagent image information on the reagent plate under illumination, an image collector processes sampling signals of the camera and transmits the processed information to a microcomputer, the microcomputer is internally provided with an expert processing system, the expert processing system can judge the result (negative or positive) of each biochemical reaction according to the colorimetric method and display the judgment result through a display screen, the automatic degree is high, the performance is stable, and the bacteria can be quickly identified to the species and the MIC quantitative analysis through perfecting an expert processing system database. The invention aims to provide a reagent plate for bacteria measurement, a device for acquiring bacteria image information and a bacteria measurement system, which are convenient to cooperate with detection equipment to carry out detection and determination one by one.
Description
Technical field
The present invention relates to microbial detection apparatus technical field, particularly the microorganisms such as the bacterium isolated and cultured, fungi are carried out kind, belong to qualification, and this bacterium of Simultaneously test is to the bacteria-measuring system detection system of the microorganism of various antibacterials MIC.
Background technology
In Micro biological Tests work, must to carry out as soon as possible kind the bacterium having cultivated out, belong to qualification, what bacterium to determine that this bacterium is? it is pathogen? it is any pathogen? the common bacterium relevant with medical science can reach hundreds of, how to complete qualification work accurately and rapidly, be pretty troublesome and stubborn problem to being engaged in this technical professional always.
Summary of the invention
Object of the present invention is exactly the deficiency for prior art, and provide a kind of be convenient to coordinate with checkout equipment carry out one by one detecting the bacteria-measuring agent plate determined.
Another object of the present invention is to provide under a kind of cavity by accommodation reagent each in agent plate is placed on light source one by one irradiates, thus completes the device of bacterium image information collecting, for the automatic detection of bacterium provides primary data data.
Of the present invention also have an object to be to provide a kind of bacteria-measuring system, its by micro computer to the initial data analysis gathered, judgement demonstrate result.
For achieving the above object, the present invention adopts following technical scheme:
Bacteria-measuring agent plate, comprising: a plate body, plate body has the multiple cavitys holding reagent, and each cavity transversely, is longitudinally spaced by setting, and there is reagent inlet cavity upper end, and cavity bottom surface is the concave spherical surface to cavity indent.
Bacteria-measuring agent plate described above, is characterized in that: described plate body is the boss of hollow, described cavity be boss table top on the downside of to the cylinder of downward-extension, cylinder and boss are structure as a whole; Concave spherical surface is bottom surface at the bottom of the cylinder of cylinder, and reagent inlet is the circular hole after cylinder bore runs through the table top of boss.
A kind of bacterium image information collecting device adopting agent plate to carry out bacteria-measuring, include constant incubator, be provided with pallet body in constant incubator, pallet body includes pallet agent plate being sent into constant incubator and the driving mechanism driving pallet transverse direction, vertically move; Have a light source above pallet body, light source irradiation is in agent plate, and a camera below agent plate, camera has faced light source straight up.
Bacterium image information collecting device described above, is characterized in that: described driving mechanism comprises base, horizontal servomotor base having a cross slide, horizontal slide rail and drive cross slide laterally to slide; Cross slide is provided with longitudinal slide rail, cross slide is also provided with the servo longitudinal motor of driving tray longitudinally slide rail longitudinal sliding motion.
Bacterium image information collecting device described above, is characterized in that: described horizontal servomotor is by the tooth bar engaged transmission on gear and cross slide; Described servo longitudinal motor is by the longitudinally slide rail slip of pallet described in belt drive.
A kind of bacteria-measuring system adopting described bacterium image information collecting device, it is characterized in that: reagent image information in agent plate under camera collection illumination, colouring information, through image acquisition device process, is sent to micro computer and judges and show by the sampled signal of camera.
The beneficial effect of hinge structure of the present invention:
1, agent plate has the multiple cavitys holding reagent, synchronously can carry out Bacteria Identification test and antibiotic sensitivity test, structure is simple, easy to use.
2, in agent plate, each cavity transversely, is longitudinally spaced by setting, adopts matrix arrangement to facilitate like this and selects the best of breed of every bacterioid, and the composite test namely placing similar different bacterium in zones of different can carry out on same reagent plate.
3, agent plate upper cavity bottom surface is the concave spherical surface to cavity indent, utilizes the sphere effect radius of camera and the concave spherical surface of cavity bottom, and the image each point sharpness that camera is absorbed is consistent, and improves the accuracy of follow-up micro computer to image recognition.
4, pallet body is provided with in constant incubator, pallet body includes pallet agent plate being sent into constant incubator and the driving mechanism driving pallet transverse direction, vertically move, a LED matrix area source is had above pallet body, light source irradiation is in agent plate, a camera below agent plate, camera has faced light source straight up, by transverse direction, vertically move agent plate, realize the mensuration image acquisition to each chamber Endophytic bacteria in agent plate, realize the unmanned automation mechanized operation intervened, substantially increase the efficiency gathering bacterium image information.
5, the present invention utilizes the growth of bacterium in constant incubator and produces the principle of color and turbidity change, cultivate when kind has the agent plate of bacterial strain to put into constant incubator kind, color and turbidity change is produced in bacteria growth process kind, LED matrix area source irradiates bottom the concave spherical surface through cavity each in agent plate uniformly, reagent image information in agent plate under camera collection illumination, the sampled signal of camera is through image acquisition device process, information after process is sent to micro computer by image acquisition device, with specialist processing system in micro computer, specialist processing system can carry out result (negative or positive) according to colorimetric ratio juris to every biochemical reaction and judge, and by display screen, result of determination is shown, its automaticity is high, stable performance, by improving specialist processing system database, bacterium can be identified rapidly kind and MIC quantitative test fast.
Accompanying drawing explanation
Fig. 1 is agent plate polycrystalline substance schematic diagram;
Fig. 2 is agent plate top structure schematic diagram;
Fig. 3 is A direction view in Fig. 2;
Fig. 4 is B-B cut-away view in Fig. 3;
Fig. 5 is C place enlarged drawing in Fig. 4;
Fig. 6 is the structural representation of pallet body top tray when longitudinally stretching out;
Fig. 7 is that pallet body top tray is retracted and structural representation after laterally sliding;
Fig. 8 is one of pallet body stereographic map;
Fig. 9 is pallet body stereographic map two;
Figure 10 is bacteria-measuring system schematic diagram of structure.
Embodiment
The technological means realized to make the present invention, creation characteristic, the object reached and effect are easily understood and are understood, and set forth the present invention further below in conjunction with accompanying drawing.
As shown in Figure 1, Figure 2, shown in Fig. 8, Fig. 9 and Figure 10, a kind of bacterium image information collecting device adopting agent plate to carry out bacteria-measuring, include constant incubator, be provided with pallet body 8 in constant incubator, pallet body 8 includes the pallet 81 agent plate 2 being sent into constant incubator and the driving mechanism 7 driving pallet 81 transverse direction, vertically move; Have a light source 1 above pallet body 8, light source 1 is LED matrix area source, and light source 1 is radiated in agent plate 2.A camera 3 below agent plate 2, camera 3 has faced light source 1 straight up.
As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 4 and Fig. 5, agent plate 2 is a plate body, plate body has the multiple cavitys 21 holding reagent, and each cavity 21 transversely, is longitudinally spaced by setting, there is reagent inlet 210 cavity 21 upper end, and cavity 21 bottom surface is the concave spherical surface 211 to cavity indent.In the present invention, plate body is the boss of hollow, cavity 21 be boss table top on the downside of to the cylinder of downward-extension, cylinder and boss are structure as a whole; Concave spherical surface 211 is bottom surface at the bottom of the cylinder of cylinder, and reagent inlet 210 runs through the circular hole after the table top of boss for cylinder bore.
As everyone knows, camera has sphere effect when picked-up plane picture, namely a sheet of planar figure takes out the sphere effect that there will be anamorphose afterwards, especially when closely absorbing micro image, focus place can be clear, focal point is more far away fuzzyyer, seems that image just has sphere sense.In Bacteria Detection kind, if the variant meeting of image definition causes the resultant error that deals comparatively large, intractability is also high.In the present invention, by the concave spherical surface that improvement cavity bottom surface is to cavity indent, make full use of camera sphere effect and obtain higher picture quality, effectively improve the accuracy of result, its method is: first by detecting the sphere effect radius of camera 3, the numerical value of sphere effect radius and camera 3 imaging focal length, then make bottom cavity 21 each in agent plate 2 and concave spherical surface 211, during work, camera 3 shooting point is placed in the centre of sphere place of concave spherical surface 211, such camera 3 shooting point is equal with the distance of each point on concave spherical surface 211, thus the image each point sharpness making camera 3 absorb is consistent, improve the accuracy of follow-up micro computer 5 pairs of image recognitions.
As shown in Fig. 6, Fig. 7, Fig. 8 and Fig. 9, driving mechanism 7 comprises base 700, base 700 has a cross slide 702, horizontal slide rail 703 and drives the cross slide 702 transversely horizontal horizontal servomotor 704 slided of slide rail 703; Cross slide 702 is provided with longitudinal slide rail 710, cross slide 702 is also provided with the servo longitudinal motor 711 of driving tray 81 longitudinally slide rail 710 longitudinal sliding motion.In the preferred embodiment for the present invention, horizontal servomotor 704 is by gear 705 and tooth bar 706 engaged transmission on cross slide 702; Servo longitudinal motor 711 by belt 713 drive pallet 81 longitudinally slide rail 710 slide.
As shown in Figure 10, a kind of bacteria-measuring system adopting bacterium image information collecting device, it utilizes the growth of bacterium in constant incubator and produces the principle of color and turbidity change, cultivate when kind has the agent plate 2 of bacterial strain to put into constant incubator kind, color and turbidity change is produced in bacteria growth process kind, LED matrix area source 1 irradiates bottom the concave spherical surface 211 through cavity 21 each in agent plate 2 uniformly, camera 3 to collect under illumination reagent image information in agent plate, utilize the concave spherical surface 211 bottom the sphere effect radius of camera 3 and cavity 21, camera 3 obtains image clearly, the sampled signal of camera 3 processes through image acquisition device 4, information after process is sent to micro computer 5 by image acquisition device 4, with specialist processing system in micro computer 5, specialist processing system can carry out result (negative or positive) according to colorimetric ratio juris to every biochemical reaction and judge, result of determination shows by display screen.
Claims (4)
1. bacterium image information collecting device, is characterized in that comprising: a plate body, plate body has the multiple cavitys holding reagent, and each cavity transversely, is longitudinally spaced by setting, and there is reagent inlet cavity upper end, and cavity bottom surface is the concave spherical surface to cavity indent; Described cavity is the cylinder to downward-extension on the downside of plate body, and described concave spherical surface is bottom surface at the bottom of the cylinder of cylinder, and described reagent inlet is the circular hole that cylinder bore upwards runs through plate body;
Be provided with pallet body in constant incubator, pallet body includes pallet agent plate being sent into constant incubator and the driving mechanism driving pallet transverse direction, vertically move; Have a light source above pallet body, light source irradiation is in agent plate, and a camera below agent plate, camera faces light source straight up.
2. bacterium image information collecting device according to claim 1, is characterized in that: described driving mechanism comprises base, horizontal servomotor base having a cross slide, horizontal slide rail and drive cross slide laterally to slide; Cross slide is provided with longitudinal slide rail, cross slide is also provided with the servo longitudinal motor of driving tray longitudinally slide rail longitudinal sliding motion.
3. bacterium image information collecting device according to claim 2, is characterized in that: described horizontal servomotor is by the tooth bar engaged transmission on gear and cross slide; Described servo longitudinal motor is by the longitudinally slide rail slip of pallet described in belt drive.
4. one kind adopts the bacteria-measuring system of bacterium image information collecting device described in claim 1, it is characterized in that: reagent image information in agent plate under camera collection illumination, colouring information, through image acquisition device process, is sent to micro computer and judges and show by the sampled signal of camera.
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| CN201110451102.1A CN102879387B (en) | 2011-12-29 | 2011-12-29 | Reagent plate for measuring bacteria, image information acquisition device, and measurement system |
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| CN201110451102.1A CN102879387B (en) | 2011-12-29 | 2011-12-29 | Reagent plate for measuring bacteria, image information acquisition device, and measurement system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103884853A (en) * | 2014-03-18 | 2014-06-25 | 珠海市银科医学工程有限公司 | Micro-pore plate drug sensitivity reader and drug sensitivity result interpretation method |
| CN107121431A (en) * | 2014-05-11 | 2017-09-01 | 卢永英 | Medical Devices bacteriologic test instrument |
| CN109030470A (en) * | 2018-05-29 | 2018-12-18 | 中国水产科学研究院珠江水产研究所 | A kind of measurement reagent of bacterial eapsular polysaccharide terminal sialic acid content and its preparation method and application |
| CN117723537B (en) * | 2023-12-18 | 2024-07-12 | 安徽省血吸虫病防治研究所 | Schistosome antibody detection result recognition equipment based on sample carrier and operation method |
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| JPH0545291A (en) * | 1991-08-19 | 1993-02-23 | Fuji Photo Film Co Ltd | Biochemical analytical device |
| JP4400778B2 (en) * | 2003-08-08 | 2010-01-20 | 株式会社エンプラス | Plastic plate and plate |
| CN2845106Y (en) * | 2005-04-19 | 2006-12-06 | 深圳市益普生医疗设备发展有限公司 | A kind of collimating apparatus gear train |
| CN201142237Y (en) * | 2008-01-11 | 2008-10-29 | 同方威视技术股份有限公司 | Regulating system of collimating device |
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| JP4153854B2 (en) * | 2003-09-26 | 2008-09-24 | テラメックス株式会社 | Analytical method using specimen and specimen analyzer equipped with internal standard for reflection measurement |
| JP2009204451A (en) * | 2008-02-28 | 2009-09-10 | Yokogawa Electric Corp | Well-plate and fluorescence imaging system using same |
| CN102023222A (en) * | 2009-09-17 | 2011-04-20 | 希森美康株式会社 | Sample processing apparatus and sample rack transporting method |
| CN101762464A (en) * | 2010-01-22 | 2010-06-30 | 珠海迪尔生物工程有限公司 | Automatic bacteria-measuring system |
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Address after: 519041 No. 19 Golden Coast Road, San Zao Town, Jin Wan District, Zhuhai, Guangdong Patentee after: Zhuhai deer Bioengineering Co.,Ltd. Address before: 519000 No. 19, Jinhai Avenue, Sanzao Town, Jinwan District, Zhuhai City, Guangdong Province Patentee before: ZHUHAI DL MEDICAL BIOTECH Co.,Ltd. |
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