CN211311459U - Automatic quick evaluation device of disinfection effect - Google Patents
Automatic quick evaluation device of disinfection effect Download PDFInfo
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- CN211311459U CN211311459U CN201921823683.5U CN201921823683U CN211311459U CN 211311459 U CN211311459 U CN 211311459U CN 201921823683 U CN201921823683 U CN 201921823683U CN 211311459 U CN211311459 U CN 211311459U
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Abstract
The utility model discloses an automatic change disinfection effect and evaluate device fast, both sides on the workstation are equipped with first support frame and second support frame respectively, be equipped with the crossbeam between first support frame and the second support frame upper portion, be equipped with automatic liquid-transfering device on the crossbeam, automatic liquid-transfering device is along the crossbeam horizontal slip, the middle part of first support frame and second support frame is equipped with the slide rail, be equipped with in the test passage with slide rail complex movable block, the test passage removes about along the slide rail through movable block, automatic liquid-transfering device is located test passage's top, magnetism adsorption equipment and constant temperature equipment are located the test passage below, magnetism adsorption equipment and constant temperature equipment are connected with control motor respectively, control motor is fixed in on the mesa, the slide rail meets with fluorescence detection device's sample platform. The advantages are that: the bacteria elution, enrichment, staining and detection are integrated together, the manual operation is simplified, the detection can be completed within 1.5 hours only by adding a sample, and the disinfection detection period is obviously shortened.
Description
Technical Field
The utility model belongs to a device that the disinfection field was used, concretely relates to automatic change disinfection effect and evaluate device fast, the enrichment of mainly being applied to living/dead fungus detects.
Background
Disinfection is a necessary measure for preventing and controlling pathogenic microorganism infection, if disinfection is not thorough, extremely serious results are likely to be caused, and therefore disinfection effect evaluation is of great significance. The evaluation of the disinfection effect by using biological indicator bacteria in the disinfection process is the most effective and reliable verification method at the present stage. According to the requirements stipulated by GB15981-1995 evaluation method and standard for disinfection and sterilization effect issued by Ministry of health, after disinfection, the sterilization is qualified when the killing rate of biological indicator bacteria is greater than or equal to 99.9%.
The traditional disinfection effect evaluation method is a plate culture counting method based on microorganism culturability, the method is long in time consumption, often needs 24-48 hours or even longer, cannot realize quick interpretation, is not suitable for on-site detection, and is easy to cause pollution in manual operation in actual detection, so that the accuracy of a detection result is influenced. The fluorescence labeling detection method is a common laboratory viable/dead bacteria distinguishing detection method, and in the application of disinfection and sterilization evaluation, operations such as elution, enrichment, separation, detection and the like need to be carried out on the exposed biological indicator, but the process design experiment operation is complex.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing an automatic change quick evaluation device of disinfection effect, it can simplify the manual operation step, realizes the automation of disinfection effect evaluation.
The technical scheme of the utility model as follows: an automatic disinfection effect rapid evaluation device comprises an automatic liquid-transferring device, a detection channel, a magnetic adsorption device, a constant temperature device, a fluorescence detection device, a control motor, a workbench, a first support frame and a second support frame, wherein the first support frame and the second support frame are respectively arranged on two sides of the workbench, a cross beam is arranged between the upper parts of the first support frame and the second support frame, the automatic liquid-transferring device is arranged on the cross beam and slides left and right along the cross beam, a slide rail is arranged in the middle of the first support frame and the second support frame, a moving slide block matched with the slide rail is arranged in the detection channel, the detection channel moves left and right along the slide rail through the moving slide block, the detection channel is used for placing a single detection packaging reagent detection card taking each evaluation detection as a unit, the automatic liquid-transferring device is positioned above the detection channel, and the magnetic adsorption device and the constant temperature, the magnetic adsorption device and the constant temperature device are respectively connected with the control motor, the control motor is fixed on the workbench, and the slide rail is connected with the sample stage of the fluorescence detection device.
The detection packaging reagent detection card is a plate-shaped component and is sequentially provided with a first sample hole, a second sample hole, a first reagent groove, a second reagent groove, a third reagent groove and a detection area.
The magnetic adsorption device is positioned below the first sample hole of the detection packaging reagent detection card.
The constant temperature device is positioned below the second sample hole of the detection packaging reagent detection card.
The fluorescence detection device is used for detecting the stained bacterial sample.
The fluorescence detection device comprises a light source, a first optical filter, a focal plane, an objective lens, a second optical filter, a focusing lens and a camera which are sequentially arranged along a light path; the light source is an LED light source, the central wavelength is 475nm, and the power is more than 1 w; the first optical filter is a band-pass optical filter, the 450-650 nm transmittance is more than 80%, and the 500-650nm transmittance is less than 0.01%; the numerical aperture of the objective lens is more than 0.2; the second optical filter is a long-wave pass optical filter, the transmittance at 400 and 500nm is less than 0.01 percent, and the transmittance at 520 and 650nm is more than 80 percent; the focal length of the focusing mirror is more than 50mm, the camera is a color area array camera, the resolution is more than 300 ten thousand pixels, and the camera is positioned at the back focal plane of the focusing mirror.
The beneficial effects of the utility model reside in that: detect with current manual operation and compare, the utility model discloses with bacterium elution, enrichment, dyeing and detection integration in an equipment, simplified manual operation greatly, the operator only needs to add the sample, can accomplish the detection in 1.5 hours, is showing and has shortened disinfection detection cycle. In the application of the actual disinfection effect evaluation, the device obtains the sterilization rate of the indicator bacteria by calculating the fluorescence points of the marked live bacteria and dead bacteria in the detection sample; the kit is simple and convenient to use, rapid in detection, accurate in result and high in sensitivity, and provides support for automatic evaluation of the disinfection effect.
Drawings
Fig. 1 is a schematic structural view of an automatic disinfection effect rapid evaluation device provided by the present invention;
FIG. 2 is a schematic view of a single test packaged reagent test card;
FIG. 3 is a schematic view of the operation path of the automatic pipetting device and the magnetic adsorption device relative to the test card;
FIG. 4 is a schematic structural diagram of a fluorescence detection device.
In the figure: the device comprises an automatic pipetting device, a detection channel, a magnetic adsorption device, a constant temperature device, a fluorescence detection device, a control motor, a workbench, a first support frame 8, a second support frame 9, a detection packaging reagent detection card 10, a first sample hole 11, a second sample hole 12, a first reagent tank 13, a second reagent tank 14, a third reagent tank 15, a detection area 16, a light source 21, a first light filter 22, a focal plane 23, an objective lens 24, a second light filter 25, a focusing lens 26 and a camera 27.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
As shown in figure 1, a structural schematic diagram of an automatic disinfection effect rapid evaluation device comprises an automatic liquid-moving device 1, a detection channel 2, a magnetic adsorption device 3, a thermostatic device 4, a fluorescence detection device 5, a control motor 6, a workbench 7, a first support frame 8 and a second support frame 9, wherein the first support frame 8 and the second support frame 9 are fixed on two sides of the workbench 7, a cross beam is arranged between the upper parts of the first support frame 8 and the second support frame 9, the automatic liquid-moving device 1 is arranged on the cross beam, the automatic liquid-moving device 1 can slide left and right along the cross beam, a slide rail is arranged in the middle parts of the first support frame 8 and the second support frame 9, a moving slide block matched with the slide rail is arranged in the detection channel 2, the detection channel 2 moves left and right along the slide rail through the moving slide block, the detection channel 2 is used for placing a single detection packaging reagent detection card, as shown in FIG. 2, the test packaged reagent test card 10 is a plate-like member having a first sample well 11, a second sample well 12, a first reagent well 13, a second reagent well 14, a third reagent well 15 and a test section 16 arranged in this order, the automatic pipetting device 1 is positioned above the test channel 2, for the pipetting operation, the magnetic adsorption device 3 and the thermostatic device 4 are positioned at the lower left of the whole device and respectively correspond to the first sample hole 11 and the second sample hole 12 of the detection packaging reagent detection card 10, the fluorescence detection device 5 is positioned at the right part of the device, wherein, the sample stage and the following light path components (specifically comprising a light source 21, a first optical filter 22 and a focal plane 23) are fixed on the working table 7, the objective lens 24 and the above light path components (specifically comprising an objective lens 24, a second optical filter 25, a focusing lens 26 and a camera 27) are connected with the first support frame 8, and the distance between the objective lens 24 and the sample stage is 10.6 mm; magnetic adsorption device 3 and constant temperature equipment 4 are connected with control motor 6 respectively, and control motor 6 is fixed in on the workstation 7, and the measuring channel 2 is located the left middle part of device, and its slide rail meets with fluorescence detection device 5's sample platform to the accurate fluorescence detection region that reachs of control detection card.
As shown in fig. 3, the thermostatic device 4 is used for providing the temperature required by the process of adsorbing bacteria by magnetic beads, and the thermostatic device is variably placed below the detection packaging reagent detection card 10 and can move up and down under the control of a motor; the utility model discloses an in the embodiment be used for when the testing process needs 37 ℃ constant temperature environment, through control motor 6 with constant temperature equipment 4 rise to suitable position, provide 37 ℃ constant temperature environment to this test tube, after the temperature is bathed, shift out the liquid after the temperature is bathed to carry out operation on next step to detecting liquid, rethread control motor 6 resumes constant temperature equipment 4 to the normal position, makes it leave the test tube.
As shown in fig. 2, detect encapsulation reagent and detect the card and place an empty test tube in first sample hole 11, place conventional reagent in second sample hole 12, first reagent groove 13 is equipped with the magnetic bead suspension of amino, and second reagent groove 14 and third reagent groove 15 are two light-resistant test tubes that are equipped with detect reagent, and the reagent card is conventional to be saved under the 4 ℃ condition in the embodiment of the utility model.
Wherein, the built-in removal slider that detects 2 can remove about the slide rail, adds the detection zone when sample pretreatment finishes, and the detection card can move to fluorescence detection device and examine test table when needing to carry out fluorescence detection, all the other moments all keep at initial position.
The fluorescence detection device 5 is used for detecting the stained bacterial sample. The method mainly realizes accurate and rapid counting of live bacteria and dead bacteria by detecting fluorescence with different wavelengths emitted by a detected dyed sample after excitation.
FIG. 4 is a schematic diagram showing the structure of the fluorescence detection apparatus. The fluorescence detection device detects the fluorescence signal of the sample to obtain the proportion of live bacteria and dead bacteria of the sample, namely the killing rate of the sterilization treatment to the indicated microorganisms (the result is presented in numerical percentage and scatter diagram modes, the killing rate is directly reflected), and the sterilization effect can be accurately evaluated according to the result.
As shown in fig. 4, the fluorescence detecting apparatus includes a light source 21, a first filter 22, a focal plane 23, an objective lens 24, a second filter 25, a focusing lens 26, and a camera 27, which are sequentially disposed along an optical path. Wherein, the light source 21 is an LED light source, the central wavelength is 475nm, and the power is more than 1 w; the first filter 22 is a band-pass filter, the transmittance at 450-650 nm is greater than 80%, and the transmittance at 500-650nm is less than 0.01%; the numerical aperture of the objective lens 24 is larger than 0.2, and the distance between the objective lens and the sample stage is 10.6 mm; the second filter 25 is a long-wave pass filter, the transmittance at 400 and 500nm is less than 0.01 percent, and the transmittance at 520 and 650nm is more than 80 percent; the focal length of the focusing mirror 26 is larger than 50mm, the camera 27 is a color area-array camera, the resolution is larger than 300 ten thousand pixels, and the camera 27 is located at the back focal plane of the focusing mirror 26.
When detecting encapsulation reagent detection card 10 and placing in sense channel 2 back, realize accurate motion through control motor 6 control, can control automatic liquid-moving device and carry out the liquid-moving operation, liquid-moving device equips subsidiary single-channel knockout for single channel single mechanical arm, can carry out the liquid-moving operation to corresponding test tube under the control of testing procedure, finally accomplish elution, enrichment, separation, dyeing and the detection integration of bacterium.
The evaluation detection device realizes data transmission between each device and an upper computer control program through the data communication module, finally directly obtains the proportion of live bacteria and dead bacteria of the sample, and can accurately evaluate the disinfection effect according to the value. In addition, the automatic disinfection effect evaluation and detection device controls the operation of each module through an upper computer program and visually displays the detection result in the form of specific numerical values and a scatter diagram so as to be convenient for an operator to check, and provides quick and accurate reference for the next work.
Claims (6)
1. The utility model provides an automatic change disinfection effect and evaluate device fast which characterized in that: the device comprises an automatic liquid-transfering device (1), a detection channel (2), a magnetic adsorption device (3), a constant temperature device (4), a fluorescence detection device (5), a control motor (6), a workbench (7), a first support frame (8) and a second support frame (9), wherein the first support frame (8) and the second support frame (9) are respectively arranged on two sides of the workbench (7), a cross beam is arranged between the upper parts of the first support frame (8) and the second support frame (9), the automatic liquid-transfering device (1) is arranged on the cross beam, the automatic liquid-transfering device (1) slides left and right along the cross beam, a slide rail is arranged in the middle parts of the first support frame (8) and the second support frame (9), a moving slide block matched with the slide rail is arranged in the detection channel (2), the detection channel (2) moves left and right along the slide rail through the moving slide block, the detection channel (2) is used for placing a single detection packaging reagent, automatic move liquid device (1) and be located the top of detecting channel (2), magnetism adsorption equipment (3) and constant temperature equipment (4) are located detecting channel (2) below, and magnetism adsorption equipment (3) and constant temperature equipment (4) are connected with control motor (6) respectively, and on control motor (6) were fixed in workstation (7), the slide rail met with the sample platform of fluorescence detection device (5).
2. An automatic disinfection effect rapid evaluation device according to claim 1, characterized in that: the detection packaging reagent detection card (10) is a plate-shaped component and is sequentially provided with a first sample hole (11), a second sample hole (12), a first reagent groove (13), a second reagent groove (14), a third reagent groove (15) and a detection area (16).
3. An automatic disinfection effect rapid evaluation device according to claim 2, wherein: the magnetic adsorption device (3) is positioned below the first sample hole (11) of the detection packaging reagent detection card (10).
4. An automatic disinfection effect rapid evaluation device according to claim 2, wherein: the constant temperature device (4) is positioned below the second sample hole (12) of the detection packaging reagent detection card (10).
5. An automatic disinfection effect rapid evaluation device according to claim 1, characterized in that: the fluorescence detection device (5) is used for detecting the stained bacterial sample.
6. An automatic disinfection effect rapid evaluation device according to claim 5, wherein: the fluorescence detection device (5) comprises a light source (21), a first optical filter (22), a focal plane (23), an objective lens (24), a second optical filter (25), a focusing lens (26) and a camera (27) which are sequentially arranged along a light path; wherein the light source (21) is an LED light source, the central wavelength is 475nm, and the power is more than 1 w; the first optical filter (22) is a band-pass filter, the transmissivity of 450-650 nm is more than 80%, and the transmissivity of 500-650nm is less than 0.01%; the numerical aperture of the objective lens (24) is larger than 0.2; the second optical filter (25) is a long-wave pass optical filter, the transmittance at 400 and 650nm is less than 0.01 percent and the transmittance at 520 and 650nm is more than 80 percent; the focal length of the focusing mirror (26) is more than 50mm, the camera (27) is a color area-array camera, the resolution is more than 300 ten thousand pixels, and the camera (27) is positioned at the back focal plane of the focusing mirror (26).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921823683.5U CN211311459U (en) | 2019-10-28 | 2019-10-28 | Automatic quick evaluation device of disinfection effect |
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| CN201921823683.5U CN211311459U (en) | 2019-10-28 | 2019-10-28 | Automatic quick evaluation device of disinfection effect |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110699241A (en) * | 2019-10-28 | 2020-01-17 | 军事科学院系统工程研究院卫勤保障技术研究所 | Automatic disinfection effect rapid evaluation device and method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110699241A (en) * | 2019-10-28 | 2020-01-17 | 军事科学院系统工程研究院卫勤保障技术研究所 | Automatic disinfection effect rapid evaluation device and method |
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