CN102870979B - Chinese herbal medicine compound feed additive for preventing red leg of bullfrog - Google Patents
Chinese herbal medicine compound feed additive for preventing red leg of bullfrog Download PDFInfo
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- CN102870979B CN102870979B CN 201210307342 CN201210307342A CN102870979B CN 102870979 B CN102870979 B CN 102870979B CN 201210307342 CN201210307342 CN 201210307342 CN 201210307342 A CN201210307342 A CN 201210307342A CN 102870979 B CN102870979 B CN 102870979B
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Abstract
The invention discloses a Chinese herbal medicine compound feed additive for preventing red leg of a bullfrog. The feed additive is prepared from a plurality of Chinese herbal medicines. The feed additive is high in palatability, low in cost and environment-friendly, can prevent red leg of the bullfrog, does not cause any toxic or side effects, and is suitable for popularization and application.
Description
Technical field
The present invention relates to the feeding additive aquatic animal field, be specifically related to a kind of Chinese herbal composite feed additive that prevents red leg of bullfrog.
Background technology
Bullfrog (the Rana catesbeiana Shaw) subphylum that belongs to vertebrate, Amphibia, Anura, Ranidae, frog subfamily.The bullfrog original producton location is North America ROCKY MOUNTAINS one band swampy area, is a kind of large edible frog, is only second to the Lin Xiwa in batrachia.Hear like ox and be the good name that obtains " bullfrog " because its song is far away.Its meat is plump, tasty, is the precious meat and fish dishes of game of a kind of high protein, LFC, and commodity is good, and successively by states such as Canada, Mexico, South America various countries and Japan, is introduced cultivation.Nineteen fifty-nine China's introduction bullfrog is raised and train, and 1986 at Central China and southern mass rearing.
Along with bullog cultivation scale in recent years constantly enlarges, in breeding process, disease is also more and more serious.In breeding process, red leg disease is the maximum a kind of communicable disease of harm, and this disease classical symptom is that bullfrog is inactive, and appetite is poor, mobility weakens, and early stage hind leg toe point is red and swollen, and blutpunkte is arranged, spread to very soon whole hind leg, simultaneously belly, lower jaw etc. are located congested rubescently, and severe patient frog toe is congested hemorrhage or fester, and cuts open inspection muscle obviously rubescent, abdominal cavity is full of ascites, liver enlargement is bronzing or yellow, spleen, kidney enlargement, and enteron aisle hyperemia is without food and be full of mucus.This disease all can occur throughout the year, mainly in popular morbidity in 4 ~ June, falls ill more anxious, death on the 2nd ~ 3 after general morbidity, and infectiousness is strong, and the death rate is high.These disease pathogenic bacteria also can cause the tadpole morbidity, tadpole anus, hind leg redness, and belly takes on a red color or hemorrhage patch etc. is arranged.At present domesticly for this disease, carried out part Study, the approach for the treatment of is all to adopt the anti-microbial type medicines such as macrolides, aminoglycoside, Tetracyclines, sulfamido, quinolones to be prevented and treated, although obtained certain effect, but these anti-microbial type medicines cause the residual of fish body on the one hand, there is in varying degrees the resistance to the action of a drug on the other hand, capital causes the resistant rate of pathogenic bacteria constantly to rise, and a large amount of multiple antibiotic resistant strains occurs, brings new challenge to the bullfrog disease prevention and cure.Up to now, about adopting Chinese herbal composite feed additive to prevent the research of red leg of bullfrog have not been reported.Therefore develop a kind of generation that can either prevent red leg of bullfrog, again can be to the noresidue of bullfrog body, the composite feed additive had no side effect, significant to intensive culture and the sustainable development of bullfrog.
Summary of the invention
The objective of the invention is, develop a kind of noresidue, be difficult for the Chinese herb feed additive developed immunity to drugs, toxic and side effect is little, is the new solution thinking of having opened up of prevention red leg of bullfrog disease.
For achieving the above object, the technical solution used in the present invention is as follows:
A kind of prevention red leg of bullfrog Chinese herbal composite feed additive, composed of the following components: polygonum flaccidum, Chinese gall, rheum officinale, the root of large-flowered skullcap, the capsule of weeping forsythia, radix bupleuri, plantain seed, the root of bidentate achyranthes, monkshood, earthworm.
In above-mentioned feed addictive, by percentage to the quality, its component is 15 ~ 25% polygonum flaccidums, 12 ~ 20% Chinese galls, 12 ~ 15% rheum officinales, 8 ~ 12% roots of large-flowered skullcap, 8 ~ 12% capsules of weeping forsythia, 10 ~ 12% radix bupleuri, 4 ~ 6% plantain seeds, 3 ~ 6% roots of bidentate achyranthes, 5 ~ 6% monkshoods, 4 ~ 5% earthworms.
A kind of method for preparing above-mentioned prevention red leg of bullfrog Chinese herbal composite feed additive, pulverize described raw material through micronizer, all by 200 purpose screen clothes, after mixing, obtain feed addictive of the present invention.
The using method of above-mentioned prevention red leg of bullfrog Chinese herbal composite feed additive, the percentage by weight according to 0.1 ~ 0.2% adds it in Bullfrog feedstuff to, mixes and gets final product.
Compared with prior art, the present invention has following advantage:
(1) to have the Chinese herbal medicine of antibacterial bacteriostatic effect be primary raw material for the preferred polygonum flaccidum of the present invention, Chinese gall, rheum officinale, the root of large-flowered skullcap, the capsule of weeping forsythia, radix bupleuri etc., guaranteeing under the prerequisite of result for the treatment of to have taken into full account the palatability problem, through optimizing compatibility, percentage by weight according to 0.1 ~ 0.2% adds additive of the present invention in Bullfrog feedstuff to, feed good palatability, bullfrog happiness food, can play prevention effect preferably, significantly reduce the red leg disease incidence of disease, improve the cultivation survival rate, increase economic efficiency.
(2) the present invention is usingd common Chinese herbal medicine as primary raw material, and China's Chinese herbal medicine resource is abundant, and raw material sources are easy.
(3) Chinese herbal medicine that the present invention selects has no side effect, and without hormone, is difficult for environment, can not affect the normal life of fish, can in the fish body, not produce residually, and non-corrosiveness, used safety, and operation is simple, with low cost, is suitable for popularization.
The specific embodiment
Further explain the present invention below in conjunction with embodiment, but embodiment does not limit in any form to the present invention.
embodiment 1
Take polygonum flaccidum 20kg, Chinese gall 15kg, rheum officinale 12kg, root of large-flowered skullcap 12kg, capsule of weeping forsythia 10kg, radix bupleuri 10kg, plantain seed 6kg, root of bidentate achyranthes 5kg, monkshood 5kg, earthworm 5kg.Through micronizer, pulverize, all through 200 purpose screen clothes, the ratio according to 0.2% after mixing is added in the bullfrog mixed feed.
embodiment 2
Take polygonum flaccidum 25kg, Chinese gall 13kg, rheum officinale 14kg, root of large-flowered skullcap 10kg, capsule of weeping forsythia 8kg, radix bupleuri 12kg, plantain seed 4kg, root of bidentate achyranthes 3kg, monkshood 6kg, earthworm 5kg.Through micronizer, pulverize, all through 200 purpose screen clothes, the ratio according to 0.2% after mixing is added in the bullfrog mixed feed.
embodiment 3
Take polygonum flaccidum 18kg, Chinese gall 17kg, rheum officinale 15kg, root of large-flowered skullcap 8kg, capsule of weeping forsythia 12kg, radix bupleuri 10kg, plantain seed 5kg, root of bidentate achyranthes 6kg, monkshood 5kg, earthworm 4kg.Through micronizer, pulverize, all through 200 purpose screen clothes, the ratio according to 0.2% after mixing is added in the bullfrog mixed feed.
embodiment 4
Take polygonum flaccidum 15kg, Chinese gall 20kg, rheum officinale 15kg, root of large-flowered skullcap 10kg, capsule of weeping forsythia 10kg, radix bupleuri 10kg, plantain seed 5kg, root of bidentate achyranthes 5kg, monkshood 5kg, earthworm 5kg.Through micronizer, pulverize, all through 200 purpose screen clothes, the ratio according to 0.2% after mixing is added in the bullfrog mixed feed.
embodiment 5
Take polygonum flaccidum 20kg, Chinese gall 12kg, rheum officinale 15kg, root of large-flowered skullcap 15kg, capsule of weeping forsythia 8kg, radix bupleuri 11kg, plantain seed 5kg, root of bidentate achyranthes 4kg, monkshood 5kg, earthworm 5kg.Through micronizer, pulverize, all through 200 purpose screen clothes, the ratio according to 0.2% after mixing is added in the bullfrog mixed feed.
the concrete application case of embodiment 6
1 experimental design
1.1 materials and methods
Three groups of feeds of the same basic of usining formula are as testing material, control group A group feed does not add Chinese herbal feed additive, B group feed adds with 0.2% dosage the Chinese herbal feed additive that patent working example 2 of the present invention is produced, and C group feed adds with 0.2% dosage the Chinese herbal feed additive that patent working example 3 of the present invention is produced.
1.2 the grouping of test bullfrog and raising
The successful bullfrog of instruction food is selected in test, be 3 groups foster after 15 days, 900 bullfrogs of the physique stalwartness of average weight 95.8 grams being divided at random temporarily, every group of 3 repetition net cages, and each repeats 100, cultivate throw something and feed respectively A described in 1.1, B, tri-groups of feeds of C separating net cage in pond.By 1 ~ 3% every morning 9:00 of bullfrog body weight and afternoon 16:00 respectively throw something and feed once.Record dead bullfrog number of elements in breeding process, and record the dead number of elements of red leg disease.After cultivating 12 weeks, bullfrog is weighed and finished test.
1.3 test data sheet and statistical analysis
Test data means with mean+SD, and each index is carried out one-way analysis of variance with SPSS 18.0 softwares, and carries out multiple ratio.
2 results and analysis
2.1 the impact of Chinese herbal feed additive on bullfrog survival rate and the red leg disease death rate
The different tests of throwing something and feeding feed is raised after 12 weeks and is finished test, and each group test bullfrog survival rate and red leg disease death rate result are as shown in table 1.
The impact of table 1 different tests material on bullfrog survival rate and the red leg disease death rate
Annotate: adopt the Duncan method relatively, the different letter representation significant differences (P<0.05) such as same row small letter abcd, the different letter representation differences such as same row capitalization ABCD are (P<0.01) extremely significantly.
Although it is not remarkable to add Chinese herbal feed additive group and control group survival rate difference, add the red leg disease death rate of two groups of Chinese herbal feed additive extremely significantly lower than control group, Chinese herbal feed additive has good prevention effect to red leg of bullfrog.
2.1 Chinese herbal feed additive is heavy on the whole opisthosoma of bullfrog and the impact of feed coefficient
The different tests of throwing something and feeding feed is raised after 12 weeks and is finished test, and the whole opisthosoma of each group test bullfrog is heavy and the feed coefficient result is as shown in table 2.
The impact of table 2 different tests material on the whole opisthosoma weight of bullfrog and feed coefficient
Annotate: adopt the Duncan method relatively, the different letter representation significant differences (P<0.05) such as same row small letter abcd, the different letter representation differences such as same row capitalization ABCD are (P<0.01) extremely significantly.
Add the heavy utmost point of the whole opisthosoma of Chinese herbal feed additive group and be significantly higher than control group, average every Frog anharmonic ratio control group exceeds 50g; Add two groups of feed coefficients of Chinese herbal feed additive extremely significantly lower than control group, feed coefficient reduces more than 0.2.
To sum up, Chinese herbal feed additive can effectively prevent the outburst of red leg of bullfrog, and Chinese herbal feed additive has the weightening finish growth-promoting effect to bullfrog simultaneously, and feed coefficient reduces more than 0.2, and very large economic potential is arranged.
Claims (3)
1. a Chinese herbal composite feed additive that prevents red leg of bullfrog, it is characterized in that being formed by the following component according to the mass percent meter: 15 ~ 25% polygonum flaccidums, 12 ~ 20% Chinese galls, 12 ~ 15% rheum officinales, 8 ~ 12% roots of large-flowered skullcap, 8 ~ 12% capsules of weeping forsythia, 10 ~ 12% radix bupleuri, 4 ~ 6% plantain seeds, 3 ~ 6% roots of bidentate achyranthes, 5 ~ 6% monkshoods, 4 ~ 5% earthworms.
2. the preparation method of the Chinese herbal composite feed additive of the described prevention red leg of bullfrog of claim 1, is characterized in that each raw material is pulverized through micronizer, by 200 eye mesh screens, after mixing, obtains.
3. the using method of the Chinese herbal composite feed additive of the described prevention red leg of bullfrog of claim 1, is characterized in that according to the ratio of 0.1 ~ 0.2 quality %, additive being added in Bullfrog feedstuff, mixes and get final product.
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104306654A (en) * | 2014-09-29 | 2015-01-28 | 浙江海洋学院 | Medicine for treating bullfrog red leg disease |
| CN104905079A (en) * | 2015-07-08 | 2015-09-16 | 青岛嘉瑞生物技术有限公司 | Bullfrog feed comprising additional enteromorpha prolifera and Chinese herbal immunopotentiating agent |
| CN105287974A (en) * | 2015-12-07 | 2016-02-03 | 天农大（天津）生物科技有限公司 | Traditional Chinese medicine composition for treating red-leg disease of bullfrogs |
| CN106562005A (en) * | 2016-11-07 | 2017-04-19 | 集美大学 | Chinese herbal medicine additive for preventing red-leg disease and skin ulcerative disease of Rana spinosa and application of Chinese herbal medicine additive |
| CN108308404A (en) * | 2018-04-09 | 2018-07-24 | 安徽龙仁堂鑫蟾生物科技有限责任公司 | A kind of Chinese herb compound feed addictive preventing toad red leg disease |
| CN108902543A (en) * | 2018-05-26 | 2018-11-30 | 谢媛 | A kind of Chinese herbal composite feed additive for treating fish sanguinicolasis |
| CN108782998B (en) * | 2018-06-04 | 2021-11-26 | 广州市联鲲生物科技有限公司 | Premix for basha fish and preparation method and application thereof |
| CN116270857A (en) * | 2023-02-14 | 2023-06-23 | 铜仁学院 | Chinese herbal medicine prevention and control method for diseases of rana nigromaculata tadpole in rice-frog co-cropping period |
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2012
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Non-Patent Citations (7)
| Title |
|---|
| 侯士良.祛风湿药.《中药八百种详解》.河南科学技术出版社,1999,第310-312页. * |
| 崔岩等.中国林蛙红腿病病原的分离鉴定及中草药药敏实验.《林业实用技术》.2007,(第2期),第32页-34页. * |
| 曾维爱等.辣蓼的应用及其功效.《中国农学通报》.2006,第22卷(第8 期),第369-372页. * |
| 王隶书等.车前子多糖研究进展.《时珍国医国药》.2012,第23卷(第2期),第460-462页. * |
| 胡金林.地龙的药理作用与临床应用.《海峡药学》.2008,第20卷(第12期),第84-86页. * |
| 金顺姬.柴胡的药理作用及临床应用.《现代医药卫生》.2009,第25卷(第7期),第1074-1075页. * |
| 陆忠康.牛蛙常见疾病的治疗.《简明中国水产养殖百科全书》.中国农业出版社,2001,第312-314页. * |
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Address after: 1011, room 890, international science and technology building, No. 510630 Tianhe North Road, Tianhe District, Guangdong, Guangzhou Patentee after: Guangdong Haid Group Co., Ltd. Patentee after: GUANGZHOU HINTER BIOTECHNOLOGY CO., LTD. Patentee after: Qingyuan Haibei Biological Technology Co., Ltd. Address before: 1011, room 890, international science and technology building, No. 510630 Tianhe North Road, Tianhe District, Guangdong, Guangzhou Patentee before: Guangdong Haid Group Co., Ltd. Patentee before: Guangzhou Hinter Bio-technology Co., Ltd. Patentee before: Qingyuan Haibei Biological Technology Co., Ltd. |