CN102835732A - Method adopting mixed enzyme preparation for improving quality of upper tobacco leaf - Google Patents
Method adopting mixed enzyme preparation for improving quality of upper tobacco leaf Download PDFInfo
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- CN102835732A CN102835732A CN2012103424745A CN201210342474A CN102835732A CN 102835732 A CN102835732 A CN 102835732A CN 2012103424745 A CN2012103424745 A CN 2012103424745A CN 201210342474 A CN201210342474 A CN 201210342474A CN 102835732 A CN102835732 A CN 102835732A
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- tobacco leaf
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- relative humidity
- cellulase
- pectase
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Abstract
The invention discloses a method adopting mixed enzyme preparation for improving quality of upper tobacco leaf. The method includes placing to-be-treated tobacco leaves in environment of 21-23 DEG C and relative humidity 58-62% for 72 h; directly adding mixed enzyme preparation, and treating in environment of 39-41 DEG C and relative humidity 58-62% for 4 h; and deactivating at 79-81 DEG C for 1 h. The mixed enzyme preparation comprises the following materials based on tobacco leaf weight: 0.6-1.2% of pectinase, 0.4-1.2% of alpha-amylase, 0.5-1.5% of acidic protease and .6-1.2% of cellulase, which have activity of 30 mu/mg, 200,000 mu/mg, 50 mu/mg and 30 mu/mg respectively. The method has the effects of simple treatment method, flexible operation and low equipment requirement. The method investigates technology for improving utilization of upper tobacco leaf, and finds an effective regulation measure for improving quality of upper tobacco leaf.
Description
Technical field:
The present invention relates to utilize biological enzyme technology to handle upper tobacco leaf, particularly relate to a kind of exogenous enzymes treated flue-cured tobacco upper tobacco leaf, to improve the method for upper tobacco leaf quality.
Background technology:
The employed raw material of cigarette enterprise is the sheet cigarette basically at present.The production of sheet cigarette need be passed through processes such as plantation, baking and beating and double roasting; Cycle is longer; Because aspects such as various places ecology, field farming measure and processing technology all exist problem more or less, the sheet cigarette also just exists little or big defective when calling in tobacco productive corporation.At present tobacco productive corporation can only remedy these defectives through the method for the nature ageing in the storehouse basically, and it is satisfied fully that effect can not be made us, and simultaneously because natural ageing generally needed for 2-3 years, also has this mouldy and risk that damage by worms at tobacco leaf during this period of time.Therefore each cigarette enterprise is all eliminated the mass defect of sheet cigarette trying to explore various novel means, and wherein biological enzyme technology is the focus of research.
Publication number is the method that the Chinese patent of CN1030862 discloses a kind of processing inferiority tobacco leaf using microbial compound enzyme; Be sprayed onto the complex microorganism enzyme liquid that contains cellulase, pectase, ligninase and protease on the discarded tobacco leaf after the moisture regain; In humidity 60-70%; Handle 22-26h under 30-50 ℃ of conditions of temperature, the inherent quality of tobacco leaf can promote 0.5-1 level; Publication number is that the Chinese patent of CN1593268 discloses a kind of cigarette with the immobilization biological additive; The emulsion that has prepared the immobilised enzymes that contains degrading proteinase, amylase, pectase, polysaccharide degrading enzyme and cellulase; And be applied in the middle of the ageing processing of redried leaf tobacco; The addition of enzyme is 8%-12%, and degradation temperature is 35-50 ℃, and degradation time is 15-18h; Publication number is that the Chinese patent of CN1723811 discloses a kind of additive for flue-cured tobacco; Be dissolved into PD enzyme, starch degrading enzyme, pectin degrading enzyme, polyphenol oxidase, cellulase, vitamin C and potassium tartrate in the water; Just formed described additive for flue-cured tobacco after the filtration; Before tobacco first baking, under the normal temperature, spray additive for flue-cured tobacco by 0.2%-5% of tobacco leaf weight, the tobacco leaf grade can improve 1-2 levels; Publication number is the method that the application of CN101040723A China discloses a kind of tobacco leaf high-activity bio-enzyme regulator and raising quality of tobacco thereof; Utilize the microbial compound enzyme and the multienzyme active regulator of the plurality of enzymes system of starch-containing enzyme, protease, cellulase and pectase; Handle the discarded tobacco leaf of roasting front and back just with this bio-enzyme regulator, inherent quality can improve 1-2 levels.Above-mentioned disclosed literature content shows the inherent quality of utilizing biological enzyme technology can improve tobacco leaf, becomes enzyme liquid but need prepare when being to use, and the method for using more complicated has increased the water content of tobacco leaf simultaneously, has also just increased the risk of tobacco mildew.
Flue-cured tobacco upper tobacco leaf cell wall substance content is higher; When sucking, be easy to generate withered and burnt smell, flue gas is coarse, excitant is bigger; Regulate the cell wall substance content of upper tobacco leaf if can utilize modern biology enzyme to learn a skill; Promote the Degradation and Transformation of cell wall substance and macromolecular substances, can reduce the assorted gas and the excitant of upper tobacco leaf; Through adding the exogenous enzymes preparation, promote the non-enzyme browning reaction that tobacco leaf is inner, improve the fragrance of tobacco leaf, further improve the industrial applicability of upper tobacco leaf.
Summary of the invention:
For overcoming the defective of prior art; The object of the present invention is to provide a kind of employing mixing enzyme preparation to improve the upper tobacco leaf method for quality; Mainly be to handle the flue-cured tobacco upper tobacco leaf, thereby improve the quality of upper tobacco leaf, improve its use value through the mode of adding enzyme preparation.
Technical solution problem of the present invention adopts following technical scheme:
A kind of employing mixing enzyme preparation improves the upper tobacco leaf method for quality, carries out according to the following steps:
A, pending tobacco leaf after 65-75 hour, is directly added mixing enzyme preparation at 20-25 ℃, 55-65% relative humidity environment held,, handled 3.5-4.5 hour under the 55-65% relative humidity at 35-45 ℃;
B, to the mixing enzyme preparation deactivation;
Said mixing enzyme preparation is made up of pectase, AMS, acid protease, cellulase, and proportion of composing is in quality of tobacco: pectase 0.6%-1.2%, AMS 0.4%-1.2%, acid protease 0.5%-1.5%, cellulase 0.6%-1.2%; Four kinds of enzymatic activitys are respectively cellulase 30u/mg, acid protease 50u/mg, pectase 30u/mg, AMS 200000u/mg.
Further, in the steps A, can pending tobacco leaf after 72 hours, directly be added mixing enzyme preparation at 22 ± 1 ℃, 60 ± 2% relative humidity environment held,, handle 4 hours under 60 ± 2% relative humidity at 40 ± 1 ℃; Deactivation 1 hour under 80 ± 1 ℃ of conditions then.
Compared with present technology, beneficial effect of the present invention is embodied in:
1. processing method is simple, and flexible operation is lower to equipment requirements.
2. the availability technology that improves upper tobacco leaf is explored, found effective adjusting measure of upper tobacco leaf quality.
3. the humiture environmental condition of pipe tobacco processing requires and can realize in redrying and throwing link, applies to have certain operability.
4. but required enzyme preparation commercialization production is widely applied secure.
Below, the present invention is described further through the specific embodiment.
The specific embodiment:
Below each embodiment all use Chenzhou, Hunan cloud 87 B
2The FA tobacco leaf is that example describes.
Embodiment 1: tobacco leaf at 20 ℃, behind the 65% relative humidity held 65h, is directly added mixing enzyme preparation, at 35 ℃, handle 3.5h under 65% relative humidity, deactivation got final product in 1 hour under 80 ± 1 ℃ of conditions.Described mixing enzyme preparation is made up of pectase, AMS, acid protease and cellulase; Proportion of composing is pectase 0.6% (accounting for tobacco leaf), AMS 0.4% (accounting for tobacco leaf), acid protease 1.5% (accounting for tobacco leaf) and cellulase 1.2% (accounting for tobacco leaf); Four kinds of enzymatic activitys are respectively cellulase 30u/mg; Acid protease 50u/mg, pectase 30u/mg, AMS 200000u/mg.
Embodiment 2: tobacco leaf at 25 ℃, behind the 65% relative humidity held 75h, is directly added mixing enzyme preparation, at 45 ℃, handle 4.5h under 55% relative humidity, deactivation got final product in 1 hour under 80 ± 1 ℃ of conditions.Described mixing enzyme preparation is made up of pectase, AMS, acid protease and cellulase; Proportion of composing is pectase 1.2% (accounting for tobacco leaf), AMS 1.2% (accounting for tobacco leaf), acid protease 0.5% (accounting for tobacco leaf) and cellulase 0.6% (accounting for tobacco leaf); Four kinds of enzymatic activitys are respectively cellulase 30u/mg; Acid protease 50u/mg, pectase 30u/mg, AMS 200000u/mg.
Embodiment 3:
Tobacco leaf at 22 ± 1 ℃, behind 60 ± 2% relative humidity held 72h, is directly added mixing enzyme preparation, at 40 ± 1 ℃, handle 4h under 60 ± 2% relative humidity, deactivation got final product in 1 hour under 80 ± 1 ℃ of conditions.Described mixing enzyme preparation is made up of pectase, AMS, acid protease and cellulase; Proportion of composing is pectase 0.9% (accounting for tobacco leaf), AMS 0.8% (accounting for tobacco leaf), acid protease 1.2% (accounting for tobacco leaf) and cellulase 0.8% (accounting for tobacco leaf); Four kinds of enzymatic activitys are respectively cellulase 30u/mg; Acid protease 50u/mg, pectase 30u/mg, AMS 200000u/mg.
Embodiment 4:
Tobacco leaf at 22 ± 1 ℃, behind 60 ± 2% relative humidity held 72h, is directly added mixing enzyme preparation, at 40 ± 1 ℃, handle 4h under 60 ± 2% relative humidity, deactivation got final product in 1 hour under 80 ± 1 ℃ of conditions.Described mixing enzyme preparation is made up of pectase, AMS, acid protease and cellulase; Proportion of composing is pectase 0.7% (accounting for tobacco leaf), AMS 0.6% (accounting for tobacco leaf), acid protease 1.0% (accounting for tobacco leaf) and cellulase 1.0% (accounting for tobacco leaf); Four kinds of enzymatic activitys are respectively cellulase 30u/mg; Acid protease 50u/mg, pectase 30u/mg, AMS 200000u/mg.
Tobacco leaf after the processing is through the evaluation of the cigarette sensory evaluation committee in the Anhui, and the result is as shown in table 1:
Aesthetic quality's score of table 1 different disposal
| Handle | Fragrance matter | Perfume quantity | Assorted gas | Strength | Concentration | Excitant | Pleasant impression | Sugariness | Total points |
| Be untreated | 7.20? | 6.00? | 7.00? | 6.00? | 6.00? | 7.00? | 6.00? | 5.80? | 72.53? |
| Embodiment 1 | 7.20? | 6.70? | 6.50? | 6.50? | 6.50? | 6.50? | 6.30? | 6.00? | 73.94? |
| Embodiment 2 | 7.20? | 6.70? | 7.00? | 6.00? | 6.50? | 7.00? | 6.50? | 6.50? | 76.06? |
| Embodiment 3 | 7.00? | 6.50? | 6.50? | 6.50? | 6.50? | 7.00? | 6.50? | 6.00? | 73.94? |
| Embodiment 4 | 7.00? | 6.50? | 6.70? | 6.50? | 6.50? | 7.00? | 6.50? | 6.00? | 74.21? |
Sensory evaluation is that index is given a mark with fragrance matter, perfume quantity, assorted gas, concentration, strength, excitant, pleasant impression, 8 of sugarinesses, and the full marks of each index are 9 fens.Total points is that index gives weight for respectively each index with fragrance matter, perfume quantity, assorted gas, excitant, pleasant impression, sugariness, and weight is as shown in table 2:
The weight of table 2 aesthetic quality index
| Index | Fragrance matter | Perfume quantity | Assorted gas | Excitant | Pleasant impression | Sugariness |
| Weight | 0.25 | 0.25 | 0.12 | 0.12 | 0.20 | 0.06 |
Aesthetic quality's score D
2=∑ (X
i* * 100/9 R), (X
iBe each index score, R is each index weight, and i is each index)
Can find out that through the data in the table 1 after the processing among four embodiment, the aesthetic quality of tobacco leaf compares the raising that has in various degree with untreated tobacco leaf, mixing enzyme preparation is directly handled Chenzhou, Hunan cloud 87 B
2The FA tobacco leaf can improve its aesthetic quality.
Table 3 has shown the chemical composition change situation through tobacco leaf after the processing of four embodiment
The chemical composition of table 3 different disposal tobacco leaf relatively
Data comparative analysis through table 3 can be found out; After the processing through the mixing enzyme preparation described in four embodiment; Compare with untreated upper tobacco leaf, unfavorable component contents such as the nicotine of tobacco leaf and pectin decrease, and total nitrogen and amino acid content rise to some extent; After the mixing enzyme preparation processing, Chenzhou, Hunan cloud 87 B
2The chemical composition of FA tobacco leaf changes to favourable direction.
Claims (2)
1. one kind is adopted mixing enzyme preparation to improve the upper tobacco leaf method for quality, it is characterized in that carrying out according to the following steps:
A, pending tobacco leaf after 65-75 hour, is directly added mixing enzyme preparation at 20-25 ℃, 55-65% relative humidity environment held,, handled 3.5-4.5 hour under the 55-65% relative humidity at 35-45 ℃;
B, to the mixing enzyme preparation deactivation;
Said mixing enzyme preparation is made up of pectase, AMS, acid protease, cellulase, and proportion of composing is in quality of tobacco: pectase 0.6%-1.2%, AMS 0.4%-1.2%, acid protease 0.5%-1.5%, cellulase 0.6%-1.2%; Four kinds of enzymatic activitys are respectively cellulase 30u/mg, acid protease 50u/mg, pectase 30u/mg, AMS 200000u/mg.
2. a kind of employing mixing enzyme preparation according to claim 1 improves the upper tobacco leaf method for quality; It is characterized in that; In the steps A, pending tobacco leaf after 72 hours, is directly added mixing enzyme preparation at 22 ± 1 ℃, 60 ± 2% relative humidity environment held; At 40 ± 1 ℃, handled 4 hours under 60 ± 2% relative humidity; Deactivation 1 hour under 80 ± 1 ℃ of conditions then.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105167141A (en) * | 2015-08-28 | 2015-12-23 | 湖南中烟工业有限责任公司 | Method for improving upper tobacco leaf usability |
| CN105266188A (en) * | 2015-11-25 | 2016-01-27 | 红云红河烟草(集团)有限责任公司 | Method for increasing permeability of feed liquid in tobacco flakes by applying complex enzyme preparation |
| CN105326086A (en) * | 2015-11-25 | 2016-02-17 | 红云红河烟草(集团)有限责任公司 | Method for increasing alcohol thickness and sweet smell of tobacco flakes by using compound enzyme preparation |
| CN105595406A (en) * | 2016-01-07 | 2016-05-25 | 河南中烟工业有限责任公司 | Compound enzyme preparation for adjusting quality of tobacco leaves and application thereof |
| CN110169587A (en) * | 2019-06-01 | 2019-08-27 | 中国烟草总公司郑州烟草研究院 | A kind of enzyme preparation application method for making to generate cinnabar shape maturation spot after tobacco leaf is roasting |
| CN111227289A (en) * | 2020-03-16 | 2020-06-05 | 河南中烟工业有限责任公司 | Method for improving baking quality of flue-cured tobacco |
| CN112586799A (en) * | 2020-12-28 | 2021-04-02 | 河南中烟工业有限责任公司 | Henan tobacco leaf rapid alcoholization control method |
| CN115721037A (en) * | 2022-12-22 | 2023-03-03 | 江苏中烟工业有限责任公司 | Method for improving applicability of upper tobacco leaf formula |
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| DE2045181A1 (en) * | 1970-09-12 | 1972-03-16 | Raban, Joachim, Dr., 2000 Schenefeld | Tobacco treatment - by proteolytic enzyme infiltration in non -fermented material |
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| CN101305836A (en) * | 2007-05-18 | 2008-11-19 | 云南瑞升烟草技术（集团）有限公司 | Method and device for preparing expanded stem granule |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105167141A (en) * | 2015-08-28 | 2015-12-23 | 湖南中烟工业有限责任公司 | Method for improving upper tobacco leaf usability |
| CN105266188A (en) * | 2015-11-25 | 2016-01-27 | 红云红河烟草(集团)有限责任公司 | Method for increasing permeability of feed liquid in tobacco flakes by applying complex enzyme preparation |
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| CN105595406A (en) * | 2016-01-07 | 2016-05-25 | 河南中烟工业有限责任公司 | Compound enzyme preparation for adjusting quality of tobacco leaves and application thereof |
| CN110169587A (en) * | 2019-06-01 | 2019-08-27 | 中国烟草总公司郑州烟草研究院 | A kind of enzyme preparation application method for making to generate cinnabar shape maturation spot after tobacco leaf is roasting |
| CN110169587B (en) * | 2019-06-01 | 2021-12-07 | 中国烟草总公司郑州烟草研究院 | Application method of enzyme preparation for producing vermilion-like mature spots after tobacco leaf baking |
| CN111227289A (en) * | 2020-03-16 | 2020-06-05 | 河南中烟工业有限责任公司 | Method for improving baking quality of flue-cured tobacco |
| CN112586799A (en) * | 2020-12-28 | 2021-04-02 | 河南中烟工业有限责任公司 | Henan tobacco leaf rapid alcoholization control method |
| CN115721037A (en) * | 2022-12-22 | 2023-03-03 | 江苏中烟工业有限责任公司 | Method for improving applicability of upper tobacco leaf formula |
| CN115721037B (en) * | 2022-12-22 | 2023-10-24 | 江苏中烟工业有限责任公司 | Method for improving applicability of upper tobacco leaf formula |
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Application publication date: 20121226 |