CN102813752A - Preparation method of creeping dichondra-berchemia lineata-verbena Gantai preparation - Google Patents
Preparation method of creeping dichondra-berchemia lineata-verbena Gantai preparation Download PDFInfo
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Abstract
The invention discloses a preparation method of creeping dichondra-berchemia lineata-verbena Gantai preparation, prepared from creeping dichondra, berchemia lineate, verbena, stephania root, herba patriniae, herba epimedii, milk vetch, red peony root, salviae miltiorrhizae and auxiliary materials. According to the preparation method disclosed by the invention, a preparation process is optimized according to properties of each medicine in a formula of creeping dichondra-berchemia lineata-verbena Gantai granules and the effect of the medicine in the formula; and compared with the prior art, the preparation method disclosed by the invention has the advantages that content of active ingredients of a product prepared by the preparation method disclosed by the invention is high and clinical effect is good.
Description
Technical field
The present invention relates to the method for preparing of the safe preparation of Golden Horse liver, belong to the field of medicine technology.
Technical background
Distending pain over the hypochondrium is scorching hot, the abdominal distention anorexia, and the bitter taste nausea, oliguria with reddish urine or Huang, it is uncomfortable to defecate, or the jaundice of body order, and red tongue with yellowish and greasy fur, wiry and frequent pulse are clinical common symptons.Soothing the liver the nourishing blood of the normal employing of the traditional Chinese medical science, the means of regulating the flow of QI to dissipate blood stasis are treated it.The traditional Chinese medical science thinks that positive QI-insufficiency then very easily infects the epidemic disease poison, and Bad Eating Habit impairing the spleen and stomach and can not removing dampness is given birth in damp and hot; Be stranded the damage of the spleen liver and show the liver and gall incoordination between the spleen and stomach; Increasing is to the damage of healthy energy, and in addition, strongly fragrant heat of a specified duration is pented up liver and gall in damp and hot heresy of diseases caused by exogenous pathogenic factor or the damp in addition; Dampness-heat in the liver and gallbladder, the acute and chronic hepatitis of caused by energy stagnation and blood stasis.Herba Dichodrae, Herba Verbenae, Herba Patriniae, the Radix Stephaniae Tetrandrae of merit that the safe preparation of Golden Horse liver is held concurrently profit with tool heat-clearing and toxic substances removing, water gallbladder is in addition with the Radix Paeoniae Rubra of Radix Salviae Miltiorrhizae, Radix berchemiae lineatae and the clearing away heat and cooling blood of the medicine Radix Astragali of QI invigorating, blood circulation promoting and blood stasis dispelling and the Herba Epimedii composition of invigorating the liver and kidney.In the side damp and hotly to be the main body accumulateing of Herba Dichodrae, Herba Verbenae, Herba Patriniae, Radix Stephaniae Tetrandrae and liver heat removing gallbladder; Herba Epimedii invigorating the liver and kidney in addition, bone and muscle strengthening; Radix berchemiae lineatae, Radix Salviae Miltiorrhizae can nourishing blood and promoting blood circulation the stasis of blood due to again can the activating QI deficiency; With Radix Astragali tonifying Qi and lifting yang, inducing diuresis to remove edema, strengthen the effect of blood circulation promoting and blood stasis dispelling, clearing away heat-damp and promoting diuresis again, alleviate the card of dampness-heat in the liver and gallbladder, qi depression to blood stasis.All medicines are aided each other, and damp and hot with the liver heat removing gallbladder, blood circulation promoting and blood stasis dispelling is main, and QI invigorating is this, and the soothing the liver blood stasis dispelling of nourishing blood again is a root, and full side's integral body and determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs reach treating both the principal and secondary aspects of a disease; Be good at removing that positive QI-insufficiency, taste are become estranged, the distending pain over the hypochondrium of dampness-heat in the liver and gallbladder, caused by energy stagnation and blood stasis is scorching hot, the abdominal distention anorexia, the bitter taste nausea, oliguria with reddish urine or Huang, it is uncomfortable to defecate, or the jaundice of body order, red tongue with yellowish and greasy fur, diseases such as wiry and frequent pulse.Modern pharmacological research proves, this prescription have protect the liver, effect such as antiinflammatory antiviral, analgesia, enhancing immunity and antithrombotic; For the treatment dampness-heat in the liver and gallbladder, the acute and chronic hepatitis of caused by energy stagnation and blood stasis has definite curative effect.The existing safe particulate method of Golden Horse liver of producing generally is to adopt drug standard (WS-10313 (ZD-0313)-2002) to go up the method for record; The method of above-mentioned standard record is fairly simple; Cause containing a large amount of invalid components and impurity in the product, make that the clinical efficacy of the safe granular preparation of Golden Horse liver is not ideal enough.
Summary of the invention:
Technical problem to be solved by this invention is to provide the method for preparing of the safe preparation of Golden Horse liver.
For solving the problems of the technologies described above, the present invention adopts following technical scheme to realize:
The safe preparation of a kind of Golden Horse liver; Calculate according to composition by weight, it is prepared from Herba Dichodrae 250-300 part, Radix berchemiae lineatae 100-200 part, Herba Verbenae 100-150 part, Radix Stephaniae Tetrandrae 150-250 part, Herba Patriniae 100-150 part, Herba Epimedii 100-200 part, Radix Astragali 100-200 part, Radix Paeoniae Rubra 100-200 part, Radix Salviae Miltiorrhizae 200-300 part and adjuvant.
Specifically; The safe preparation of said Golden Horse liver calculates according to composition by weight, and it is prepared from 288.2 parts of Herba Dichodraees, 153.3 parts of Radix berchemiae lineataes, 115 parts of Herba Verbenae, 191.6 parts of Radix Stephaniae Tetrandrae, 115 parts of Herba Patriniae, 153.3 parts of Herba Epimedii, 153.3 parts of the Radixs Astragali, 153.3 parts of Radix Paeoniae Rubra, 230 parts of Radix Salviae Miltiorrhizaes and adjuvant.
The method for preparing of the safe preparation of said Golden Horse liver is: get Herba Epimedii and pulverize, use alcohol reflux, filter, reclaim ethanol, filtrating simmer down to thick paste; Herba Dichodrae is pulverized, and carries with alcohol reflux, filters filtrate recycling ethanol and simmer down to thick paste; The Radix Stephaniae Tetrandrae crushed after being dried is a coarse powder, adopts alcohol reflux, and extracting solution reclaims ethanol to there not being the alcohol flavor, under agitation dropwise adds hydrochloric acid; Make PH=5, leave standstill, filter; Transfer PH=9 with strong aqua ammonia, leave standstill the back sucking filtration, the acetone heating for dissolving is used in the dry back of deposition; Adding distil water to solution is little turbid in solution, reclaims acetone and is condensed into thick paste, and is subsequent use; All the other Radix berchemiae lineataes, Herba Verbenae, Herba Patriniae, the Radix Astragali, Radix Paeoniae Rubra and Radix Salviae Miltiorrhizae decocte with water, decocting liquid filters, and filtrating is condensed into clear paste, is chilled to room temperature, adds ethanol, stirs, and leaves standstill, filter, filtrate recycling ethanol, medicinal liquid is condensed into thick paste; Merge above-mentioned thick paste, add conventional adjuvant again and can process different oral formulations according to conventional method.
Specifically, the method for preparing of the safe preparation of said Golden Horse liver is: get the Herba Epimedii pulverizing and be coarse powder, add 30 times of amount 70% alcohol reflux 2 times, each 1.75h filters, recovery ethanol, and relative density was 1.34～1.40 thick paste when filtrating was concentrated into 50～60 ℃; Herba Dichodrae is pulverized and to be coarse powder, and with 6 times of amount 90% alcohol reflux 3 times, each 1h filters, merging filtrate, recovery ethanol and when being concentrated into 50～60 ℃ relative density be 1.34～1.40 thick paste; The Radix Stephaniae Tetrandrae crushed after being dried is a coarse powder, adopts 85% alcohol reflux 3 times, each 1 hour, and merge extractive liquid; Reclaim ethanol to there not being the alcohol flavor, under agitation dropwise add 1% hydrochloric acid, make PH=5, leave standstill 48h; Filter, transfer PH=9 with strong aqua ammonia, leave standstill sucking filtration behind the 48h, the acetone heating for dissolving are measured with 2 times in the dry back of deposition; Adding distil water to solution is little turbid in solution, and relative density is 1.34～1.40 thick paste when reclaiming acetone and being concentrated into 50～60 ℃, and is subsequent use; All the other Radix berchemiae lineataes, Herba Verbenae, Herba Patriniae, the Radix Astragali, Radix Paeoniae Rubra and Radix Salviae Miltiorrhizae decocte with water twice, each 1.5 hours, collecting decoction; Filter, it is 1.14～1.16 clear paste up to relative density that filtrating concentrates 90～95 ℃, is chilled to room temperature; Add 2 times of amount ethanol, stir, placed 48 hours; Filter, relative density was 1.34～1.40 thick paste when filtrate recycling ethanol, medicinal liquid were concentrated into 50～60 ℃; Merge above-mentioned thick paste, add conventional adjuvant again and can process different oral formulations according to conventional method.
Said oral formulations is meant granule, capsule or tablet.
Said granule prepares like this: get Herba Epimedii and pulverize, use alcohol reflux, filter, reclaim ethanol, filtrating simmer down to thick paste; Herba Dichodrae is pulverized, and carries with alcohol reflux, filters filtrate recycling ethanol and simmer down to thick paste; The Radix Stephaniae Tetrandrae crushed after being dried is a coarse powder, adopts alcohol reflux, and extracting solution reclaims ethanol to there not being the alcohol flavor, under agitation dropwise adds hydrochloric acid; Make PH=5, leave standstill, filter; Transfer PH=9 with strong aqua ammonia, leave standstill the back sucking filtration, the acetone heating for dissolving is used in the dry back of deposition; Adding distil water to solution is little turbid in solution, reclaims acetone and is condensed into thick paste, and is subsequent use; All the other Radix berchemiae lineataes, Herba Verbenae, Herba Patriniae, the Radix Astragali, Radix Paeoniae Rubra and Radix Salviae Miltiorrhizae decocte with water, decocting liquid filters, and filtrating is condensed into clear paste, is chilled to room temperature, adds ethanol, stirs, and leaves standstill, filter, filtrate recycling ethanol, medicinal liquid is condensed into thick paste; Merge above-mentioned thick paste, pulverize, add the dextrin of the amount of processing 60%, spray into 2% hydroxypropyl emthylcellulose ethanol liquid 80m1 and granulate, packing promptly gets.
Said capsule prepares like this: get Herba Epimedii and pulverize, use alcohol reflux, filter, reclaim ethanol, filtrating simmer down to thick paste; Herba Dichodrae is pulverized, and carries with alcohol reflux, filters filtrate recycling ethanol and simmer down to thick paste; The Radix Stephaniae Tetrandrae crushed after being dried is a coarse powder, adopts alcohol reflux, and extracting solution reclaims ethanol to there not being the alcohol flavor, under agitation dropwise adds hydrochloric acid; Make PH=5, leave standstill, filter; Transfer PH=9 with strong aqua ammonia, leave standstill the back sucking filtration, the acetone heating for dissolving is used in the dry back of deposition; Adding distil water to solution is little turbid in solution, reclaims acetone and is condensed into thick paste, and is subsequent use; All the other Radix berchemiae lineataes, Herba Verbenae, Herba Patriniae, the Radix Astragali, Radix Paeoniae Rubra and Radix Salviae Miltiorrhizae decocte with water, decocting liquid filters, and filtrating is condensed into clear paste, is chilled to room temperature, adds ethanol, stirs, and leaves standstill, filter, filtrate recycling ethanol, medicinal liquid is condensed into thick paste; Merge above-mentioned thick paste, 60 ℃ of dryings are pulverized, and add the magnesium stearate of the amount of processing 0.5%, and mixing incapsulates, and promptly gets.
Said tablet prepares like this: get Herba Epimedii and pulverize, use alcohol reflux, filter, reclaim ethanol, filtrating simmer down to thick paste; Herba Dichodrae is pulverized, and carries with alcohol reflux, filters filtrate recycling ethanol and simmer down to thick paste; The Radix Stephaniae Tetrandrae crushed after being dried is a coarse powder, adopts alcohol reflux, and extracting solution reclaims ethanol to there not being the alcohol flavor, under agitation dropwise adds hydrochloric acid; Make PH=5, leave standstill, filter; Transfer PH=9 with strong aqua ammonia, leave standstill the back sucking filtration, the acetone heating for dissolving is used in the dry back of deposition; Adding distil water to solution is little turbid in solution, reclaims acetone and is condensed into thick paste, and is subsequent use; All the other Radix berchemiae lineataes, Herba Verbenae, Herba Patriniae, the Radix Astragali, Radix Paeoniae Rubra and Radix Salviae Miltiorrhizae decocte with water, decocting liquid filters, and filtrating is condensed into clear paste, is chilled to room temperature, adds ethanol, stirs, and leaves standstill, filter, filtrate recycling ethanol, medicinal liquid is condensed into thick paste; Merge above-mentioned thick paste, add the starch of the amount of processing 32% and 1.3% microcrystalline Cellulose again, granulate, 60 ℃ of dryings, the magnesium stearate of the adding amount of processing 0.1%, mixing, tabletting, the bag film-coat promptly gets.
In the drug prescription according to the invention; Principal agent Herba Dichodrae in the safe granule prescription of Golden Horse liver has the merit that heat-clearing and toxic substances removing, function of gallbladder promoting, diuretic are invigorated blood circulation, and its main effective ingredient is flavonoid and saccharide, and pharmacological research shows that wherein flavonoid and saccharide have and protects the liver and the enhancing immunity effect; Directly use water boiling and extraction in the prior art; Making that water insoluble active ingredient such as flavone are big type just extracts not come out, and the mode that has caused water directly to decoct makes extract can not guarantee the effect of crude drug, so the method that the applicant adopts alcohol heating reflux to extract is extracted; Can most sugar be extracted, can guarantee the content of flavonoid and sugar effectively.To have the effective ingredient that the merit of inducing diuresis to remove edema, pain relieving comes from wherein be alkaloids to Radix Stephaniae Tetrandrae in the side; Existing is directly to use water extraction in clinical, causes that wherein effective ingredient alkaloid extraction rate is not high.Herba Epimedii has the effect of invigorating the liver and kidney, bone and muscle strengthening, and its main effective ingredient of modern study proof is Herba Epimedii total flavones and epimedium brevicornum polysaccharide; Through a large amount of experimentatioies, adopt the circumfluence method extraction to obtain better effects, reflux, extract, can keep Herba Epimedii exclusive effect in prescription separately.The extractum of gained adopts cold drying, has avoided the inactivation of effective ingredient, preferred technology not only to reduce the extraction difficulty with cost but also guaranteed the original effect of extract maintenance medical material with general high temperature drying method.
The applicant has carried out following experiment, and provable the present invention has effective effect;
Experimental example 1: Herba Dichodrae Study on extraction
The applicant adopts diverse ways to extract Herba Dichodrae, and the result is following:
The yield of the different extracting modes of crude polysaccharides and content are relatively in table 1 Herba Dichodrae
The result shows: the crude polysaccharides effect is preferable in the heating reflux method extraction Herba Dichodrae.
Experimental example 2: Herba Epimedii Study on extraction
The present invention is that index is carried out preferably the extraction process of Herba Epimedii with the yield of icariin, and technological level factor table is seen table 2, A: liquid material ratio, B: extraction time (h), C: concentration of alcohol (%).
Table 2 horizontal factor table
Find after deliberation: the optimum extraction process of Herba Epimedii is: A
3B
2C
2Carry out confirmatory experiment again, extract 3 times, recording the average yield of icariin is 1.36 μ gg, and RSD is 3.51%, and the result sees table 3:
Table 3 replication experiment result
The optimum extraction process condition of the preferred Herba Epimedii of experimentation is: add 30 times of amounts of medical material, 70% alcohol reflux 2 times, each 1.75h.
Experimental example 3: Study on extraction in the Radix Stephaniae Tetrandrae
According to modern pharmacology research, the total alkaloids in the Radix Stephaniae Tetrandrae has antiinflammatory action, and therefore, the present invention has done research and improved technology the extraction of total alkaloids in the Radix Stephaniae Tetrandrae, and method and result are following:
A: with 60% alcohol reflux 3 times, each 1 hour, merge extractive liquid; Reclaim ethanol to there not being the alcohol flavor, under agitation dropwise add 1% hydrochloric acid, make PH=5; Leave standstill 48h and filter, transfer PH=9, leave standstill sucking filtration behind the 48h with strong aqua ammonia; Filtrating reuse chloroform extracts repeatedly, removes water-soluble portion, reclaims chloroform and gets total alkaloids.
B: adopt 85% alcohol reflux 3 times, each 1 hour, merge extractive liquid; Reclaim ethanol to there not being the alcohol flavor, under agitation dropwise add 1% hydrochloric acid, make PH=5; Leave standstill 48h and filter, transfer PH=9, leave standstill sucking filtration behind the 48h with strong aqua ammonia; The dry back of deposition is with 2 times of amount acetone heating for dissolving, and it is little turbid in solution, to drip distilled water to solution, places and waits to dissolve to volatilize to obtain crystallization.
C: decocte with water twice, each 1.5 hours, collecting decoction; Filter, filtrating is concentrated into the clear paste that relative density is 1.14～1.16 (90～95 ℃), is chilled to room temperature; Add 2 times of amount ethanol, stir, placed 48 hours; Filter, filtrate recycling ethanol, medicinal liquid are concentrated into the thick paste of relative density 1.34～1.40 (50～60 ℃).
Result: see table 4.
Table 4 Different Extraction Method total alkaloids recovery rate
Experimental result shows: the total alkaloids recovery rate of B method is high, and does not use harmful Reagent Chloroform, and technology relatively has realistic meaning.
Experimental example 4: pharmacodynamic study
The applicant has carried out main pharmacodynamics research to the present invention, through experimental data beneficial effect of the present invention is described.
1. antiinflammatory experiment
1.1 experimental animal, material and medicine
Kunming mice, 18～22g, the male and female dual-purpose is purchased in the Medical University Of Chongqing experimental center, the quality certification number 0000112.(Shanghai reagent one is long, lot number: 1101101) for JN-A type precision torsion balance (Shanghai Second Balance Factory), xylene.
Former technology extract: the method that goes up record according to drug standard (WS-10313 (ZD-0313)-2002) is processed extract.
Extract of the present invention: the method according to the embodiment of the invention 1 is prepared into extract.
1.2 test method
Half and half 60 mices of male and female are divided into 3 groups at random, respectively gastric infusion.Matched group is given NS, former technology extract group (8.2gkg
-1), extract (8.2gkg of the present invention
-1) group.Every day 1 time, totally 7 days; 1h after the last administration, each is organized the wide two sides of the equal auris dextra of mice and evenly is coated with every 0.05ml of xylene, and left ear not Tu is a normal ear, causes 1h after the scorching swelling, takes off neck and puts to death, and two ears about under the auricle edge scissor are put precision torsion balance and are weighed at once.Heavily deduct left auricle recuperation to the swelling net value with the auris dextra sheet, and calculate the swollen suppression ratio of ear.Suppression ratio (%)=(the average swelling value of the matched group-average swelling value of administration group)/average swelling value of matched group *
100%。
1.3 experimental result
Table 5 is respectively organized the influence of extract to mice ear
Compare with NS group, extract group of the present invention and, former technology extract group relatively, < 0.05, the result shows: the present invention's extract of writing out a prescription more effectively suppresses xylene induced mice auricle inflammatory edema to P.
1.4 conclusion
Above experimental studies results shows: preparation of the present invention is to inflammation swelling tool anti-inflammation detumescence, antiinflammatory action, and antiinflammatory antiinflammatory effect is superior to former technology.
2. analgesic test
2.1 experimental technique
Get 48 of healthy Kunming mouses; Male and female half and half; Be divided into 4 groups at random; Be normal saline group, extract group of the present invention (processing extract), former technology extract group (technology according to the record of ministry standard MAIWEIDIHUANGPIAN is processed extract) and aspirin group according to the method for the invention, 15 every group.Successive administration 7 days, every day 1 time, the normal saline group gives equal-volume normal saline (20ml/kg); All the other groups press respectively 0.4g/Kg (20ml/kg) dosage irritate to obey preparation of the present invention, former technology preparation and aspirin medicinal liquid (0.2g/kg, 20ml/kg), all in last 30min after administration; Each mouse peritoneal is only injected 0.8% acetum 0.2ml/; The number of times of writhing response appears in every mice in the observed and recorded 20min, calculates mean and standard deviation, the comparable group differences.
2.2 result
Each administration group all can be resisted the pain reaction due to the acetic acid stimulation, makes the mouse writhing number of times obviously reduce (seeing table 6).
The influence of table 6 pair mouse writhing number of times (n=15, x ± s)
Annotate: extract group of the present invention and former technology extract group be P＜0.05 relatively
2.3 conclusion
Above experimental studies results shows that extract group of the present invention and former technology extract group all can be resisted the pain reaction due to the acetic acid stimulation; Extract group wherein of the present invention is superior to former technology extract group (P＜0.05).
3 protect the liver experimentation
3.1 experimental animal and material
110 of kunming mices, 18～22g, the male and female dual-purpose is purchased in the Medical University Of Chongqing experimental center, the quality certification number 0000112.(Shanghai reagent one is long, lot number: 1101101) for JN-A type precision torsion balance (Shanghai Second Balance Factory), carbon tetrachloride.
3.2 test method
40 of mices, male and female half and half, be divided at random the normal control group (distilled water, 20ml/kg); Model group (distilled water, 20ml/kg), extract group of the present invention (0.4g/kg); 10 every group of former technology extract groups (0.4g/kg), every day, gastric infusion was 1 time, 1 week of successive administration; Except that the normal control group, each is organized mouse peritoneal and injects 1% carbon tetrachloride peanut oil solution 10ml/kg body weight behind the last administration 1h.Get blood from the mouse orbit venous plexus behind the 20h, separation of serum.Adopt the clinical reagent cassette method to measure serum alanine aminotransferase (ALT), aspartate amino transferase (AST) activity, total serum protein (TP) and albumin (ALB) content.Relatively each group and the significant difference of model group are estimated the write out a prescription function for protecting liver and reducing enzyme activity of extract of the present invention.
3.3 experimental result
Experimental result sees the following form.The result shows, with matched group relatively, the ALT of model group serum, AST activity be all than the obvious rising of matched group, shows modeling success (P < 0.01); Drug group can obviously reduce carbon tetrachloride cause ALT in the hepatic injury mice serum, AST active (P 0.05 or P 0.01); And total protein and albuminous content in the serum that can obviously raise (P 0.05 or P 0.01); Wherein, Compare with former technology extract group, the effect of extract group of the present invention is better.
Two kinds of different process of table 7 prescription extract causes the protection (x ± s) of hepatic injury mice to carbon tetrachloride
Annotate: compare with model group,
*P 0.05,
*P<0.01; Compare with former technology extract group,
#P<0.05.
3.4 experiment conclusion
Test the result factually, compare extract group better effects if of the present invention with former technology extract group.
Compared with prior art, the present invention adopts the method for alcohol heating reflux to extract Herba Dichodrae, can guarantee the content of flavonoid and sugar effectively; Adopt the method for the invention to extract Radix Stephaniae Tetrandrae, can effectively extract alkaloid; Adopt circumfluence method to extract Herba Epimedii, the extraction ratio of icariin is higher.The present invention is according to the character of each medicine in the safe granule side of Golden Horse liver; And this medicine role in prescription; Technology to the safe preparation of Golden Horse liver is optimized, and makes the refine more of this medicine, and drug effect is more remarkable; For dampness-heat in the liver and gallbladder, the acute and chronic hepatitis of caused by energy stagnation and blood stasis has better therapeutical effect.Compare with existing preparation, preparation active constituent content of the present invention is high, and clinical efficacy is good, has reached the purpose of invention.
The specific embodiment:
Embodiment 1:
Prescription: Herba Dichodrae 288.2g, Radix berchemiae lineatae 153.3g, Herba Verbenae 115g, Radix Stephaniae Tetrandrae 191.6g, Herba Patriniae 115g, Herba Epimedii 153.3g, Radix Astragali 153.3g, Radix Paeoniae Rubra 153.3g, Radix Salviae Miltiorrhizae 230g.
Technology: said granule prepares like this: get the Herba Epimedii pulverizing and be coarse powder, add 30 times of amount 70% alcohol reflux 2 times, each 1.75h filters, recovery ethanol, and relative density was 1.34～1.40 thick paste when filtrating was concentrated into 50～60 ℃; Herba Dichodrae is pulverized and to be coarse powder, and with 6 times of amount 90% alcohol reflux 3 times, each 1h filters, merging filtrate, recovery ethanol and when being concentrated into 50～60 ℃ relative density be 1.34～1.40 thick paste; The Radix Stephaniae Tetrandrae crushed after being dried is a coarse powder, adopts 85% alcohol reflux 3 times, each 1 hour, and merge extractive liquid; Reclaim ethanol to there not being the alcohol flavor, under agitation dropwise add 1% hydrochloric acid, make PH=5, leave standstill 48h; Filter, transfer PH=9 with strong aqua ammonia, leave standstill sucking filtration behind the 48h, the acetone heating for dissolving are measured with 2 times in the dry back of deposition; Adding distil water to solution is little turbid in solution, and relative density is 1.34～1.40 thick paste when reclaiming acetone and being concentrated into 50～60 ℃, and is subsequent use; All the other Radix berchemiae lineataes, Herba Verbenae, Herba Patriniae, the Radix Astragali, Radix Paeoniae Rubra and Radix Salviae Miltiorrhizae decocte with water twice, each 1.5 hours, collecting decoction; Filter, it is 1.14～1.16 clear paste up to relative density that filtrating concentrates 90～95 ℃, is chilled to room temperature; Add 2 times of amount ethanol, stir, placed 48 hours; Filter, relative density was 1.34～1.40 thick paste when filtrate recycling ethanol, medicinal liquid were concentrated into 50～60 ℃; Merge above-mentioned thick paste, pulverize, add the dextrin of the amount of processing 60%, spray into 2% hydroxypropyl emthylcellulose ethanol liquid 80m1 and granulate, packing promptly gets.
Specification: every bag of 10g.
Usage and consumption: boiled water is taken after mixing it with water, one time 1 bag, 3 times on the one.
Embodiment 2:
Prescription: Herba Dichodrae 300g, Radix berchemiae lineatae 200g, Herba Verbenae 150g, Radix Stephaniae Tetrandrae 250g, Herba Patriniae 150g, Herba Epimedii 200g, Radix Astragali 200g, Radix Paeoniae Rubra 200g, Radix Salviae Miltiorrhizae 300g.
Technology: said capsule prepares like this: get the Herba Epimedii pulverizing and be coarse powder, add 30 times of amount 70% alcohol reflux 2 times, each 1.75h filters, recovery ethanol, and relative density was 1.34～1.40 thick paste when filtrating was concentrated into 50～60 ℃; Herba Dichodrae is pulverized and to be coarse powder, and with 6 times of amount 90% alcohol reflux 3 times, each 1h filters, merging filtrate, recovery ethanol and when being concentrated into 50～60 ℃ relative density be 1.34～1.40 thick paste; The Radix Stephaniae Tetrandrae crushed after being dried is a coarse powder, adopts 85% alcohol reflux 3 times, each 1 hour, and merge extractive liquid; Reclaim ethanol to there not being the alcohol flavor, under agitation dropwise add 1% hydrochloric acid, make PH=5, leave standstill 48h; Filter, transfer PH=9 with strong aqua ammonia, leave standstill sucking filtration behind the 48h, the acetone heating for dissolving are measured with 2 times in the dry back of deposition; Adding distil water to solution is little turbid in solution, and relative density is 1.34～1.40 thick paste when reclaiming acetone and being concentrated into 50～60 ℃, and is subsequent use; All the other Radix berchemiae lineataes, Herba Verbenae, Herba Patriniae, the Radix Astragali, Radix Paeoniae Rubra and Radix Salviae Miltiorrhizae decocte with water twice, each 1.5 hours, collecting decoction; Filter, it is 1.14～1.16 clear paste up to relative density that filtrating concentrates 90～95 ℃, is chilled to room temperature; Add 2 times of amount ethanol, stir, placed 48 hours; Filter, relative density was 1.34～1.40 thick paste when filtrate recycling ethanol, medicinal liquid were concentrated into 50～60 ℃; Merge above-mentioned thick paste, 60 ℃ of dryings are pulverized, and add the magnesium stearate of the amount of processing 0.5%, and mixing incapsulates, and promptly gets.
Specification: 0.3g/ grain.
Usage and consumption: oral, each 2,3 times on the one.
Embodiment 3:
Prescription: Herba Dichodrae 250g, Radix berchemiae lineatae 100g, Herba Verbenae 100g, Radix Stephaniae Tetrandrae 150g, Herba Patriniae 100g, Herba Epimedii 100g, Radix Astragali 100g, Radix Paeoniae Rubra 100g, Radix Salviae Miltiorrhizae 200g.
Technology: get the Herba Epimedii pulverizing and be coarse powder, add 30 times of amount 70% alcohol reflux 2 times, each 1.75h filters, recovery ethanol, and relative density was 1.34～1.40 thick paste when filtrating was concentrated into 50～60 ℃; Herba Dichodrae is pulverized and to be coarse powder, and with 6 times of amount 90% alcohol reflux 3 times, each 1h filters, merging filtrate, recovery ethanol and when being concentrated into 50～60 ℃ relative density be 1.34～1.40 thick paste; The Radix Stephaniae Tetrandrae crushed after being dried is a coarse powder, adopts 85% alcohol reflux 3 times, each 1 hour, and merge extractive liquid; Reclaim ethanol to there not being the alcohol flavor, under agitation dropwise add 1% hydrochloric acid, make PH=5, leave standstill 48h; Filter, transfer PH=9 with strong aqua ammonia, leave standstill sucking filtration behind the 48h, the acetone heating for dissolving are measured with 2 times in the dry back of deposition; Adding distil water to solution is little turbid in solution, and relative density is 1.34～1.40 thick paste when reclaiming acetone and being concentrated into 50～60 ℃, and is subsequent use; All the other Radix berchemiae lineataes, Herba Verbenae, Herba Patriniae, the Radix Astragali, Radix Paeoniae Rubra and Radix Salviae Miltiorrhizae decocte with water twice, each 1.5 hours, collecting decoction; Filter, it is 1.14～1.16 clear paste up to relative density that filtrating concentrates 90～95 ℃, is chilled to room temperature; Add 2 times of amount ethanol, stir, placed 48 hours; Filter, relative density was 1.34～1.40 thick paste when filtrate recycling ethanol, medicinal liquid were concentrated into 50～60 ℃; Merge above-mentioned thick paste, add the starch of the amount of processing 32% and 1.3% microcrystalline Cellulose again, granulate, 60 ℃ of dryings, the magnesium stearate of the adding amount of processing 0.1%, mixing, tabletting, the bag film-coat promptly gets.
Specification: the heavy 0.3g of substrate.
Usage and consumption: oral, each 2,3 times on the one.
Claims (6)
1. the method for preparing of the safe preparation of a Golden Horse liver; It is characterized in that: calculate according to composition by weight; It is prepared from according to following method Herba Dichodrae 250-300 part, Radix berchemiae lineatae 100-200 part, Herba Verbenae 100-150 part, Radix Stephaniae Tetrandrae 150-250 part, Herba Patriniae 100-150 part, Herba Epimedii 100-200 part, Radix Astragali 100-200 part, Radix Paeoniae Rubra 100-200 part, Radix Salviae Miltiorrhizae 200-300 part and adjuvant: Herba Epimedii is pulverized, and uses alcohol reflux, filters; Reclaim ethanol, filtrating simmer down to thick paste; Get Herba Epimedii and pulverize, use alcohol reflux, filter, reclaim ethanol, filtrating simmer down to thick paste; Herba Dichodrae is pulverized, and carries with alcohol reflux, filters filtrate recycling ethanol and simmer down to thick paste; The Radix Stephaniae Tetrandrae crushed after being dried is a coarse powder, adopts alcohol reflux, and extracting solution reclaims ethanol to there not being the alcohol flavor, under agitation dropwise adds hydrochloric acid; Make PH=5, leave standstill, filter; Transfer PH=9 with strong aqua ammonia, leave standstill the back sucking filtration, the acetone heating for dissolving is used in the dry back of deposition; Adding distil water to solution is little turbid in solution, reclaims acetone and is condensed into thick paste, and is subsequent use; All the other Radix berchemiae lineataes, Herba Verbenae, Herba Patriniae, the Radix Astragali, Radix Paeoniae Rubra and Radix Salviae Miltiorrhizae decocte with water, decocting liquid filters, and filtrating is condensed into clear paste, is chilled to room temperature, adds ethanol, stirs, and leaves standstill, filter, filtrate recycling ethanol, medicinal liquid is condensed into thick paste; Merge above-mentioned thick paste, add conventional adjuvant again and can process different oral formulations according to conventional method.
2. the method for preparing of the safe preparation of Golden Horse liver according to claim 1 is characterized in that: get Herba Epimedii and pulverize and be coarse powder, adds 30 times and measure 70% alcohol reflux 2 times; Each 1.75h; Filter, reclaim ethanol, relative density was 1.34～1.40 thick paste when filtrating was concentrated into 50～60 ℃; Herba Dichodrae is pulverized and to be coarse powder, and with 6 times of amount 90% alcohol reflux 3 times, each 1h filters, merging filtrate, recovery ethanol and when being concentrated into 50～60 ℃ relative density be 1.34～1.40 thick paste; The Radix Stephaniae Tetrandrae crushed after being dried is a coarse powder, adopts 85% alcohol reflux 3 times, each 1 hour, and merge extractive liquid; Reclaim ethanol to there not being the alcohol flavor, under agitation dropwise add 1% hydrochloric acid, make PH=5, leave standstill 48h; Filter, transfer PH=9 with strong aqua ammonia, leave standstill sucking filtration behind the 48h, the acetone heating for dissolving are measured with 2 times in the dry back of deposition; Adding distil water to solution is little turbid in solution, and relative density is 1.34～1.40 thick paste when reclaiming acetone and being concentrated into 50～60 ℃, and is subsequent use; All the other Radix berchemiae lineataes, Herba Verbenae, Herba Patriniae, the Radix Astragali, Radix Paeoniae Rubra and Radix Salviae Miltiorrhizae decocte with water twice, each 1.5 hours, collecting decoction; Filter, it is 1.14～1.16 clear paste up to relative density that filtrating concentrates 90～95 ℃, is chilled to room temperature; Add 2 times of amount ethanol, stir, placed 48 hours; Filter, relative density was 1.34～1.40 thick paste when filtrate recycling ethanol, medicinal liquid were concentrated into 50～60 ℃; Merge above-mentioned thick paste, add conventional adjuvant again and can process different oral formulations according to conventional method.
3. according to the method for preparing of claim 1 or the safe preparation of 2 said Golden Horse livers, it is characterized in that: said oral formulations is meant granule, capsule or tablet.
4. according to the method for preparing of the safe preparation of the said Golden Horse liver of claim 3, it is characterized in that: said granule prepares like this: get Herba Epimedii and pulverize, use alcohol reflux, filter, reclaim ethanol, filtrating simmer down to thick paste; Herba Dichodrae is pulverized, and carries with alcohol reflux, filters filtrate recycling ethanol and simmer down to thick paste; The Radix Stephaniae Tetrandrae crushed after being dried is a coarse powder, adopts alcohol reflux, and extracting solution reclaims ethanol to there not being the alcohol flavor, under agitation dropwise adds hydrochloric acid; Make PH=5, leave standstill, filter; Transfer PH=9 with strong aqua ammonia, leave standstill the back sucking filtration, the acetone heating for dissolving is used in the dry back of deposition; Adding distil water to solution is little turbid in solution, reclaims acetone and is condensed into thick paste, and is subsequent use; All the other Radix berchemiae lineataes, Herba Verbenae, Herba Patriniae, the Radix Astragali, Radix Paeoniae Rubra and Radix Salviae Miltiorrhizae decocte with water, decocting liquid filters, and filtrating is condensed into clear paste, is chilled to room temperature, adds ethanol, stirs, and leaves standstill, filter, filtrate recycling ethanol, medicinal liquid is condensed into thick paste; Merge above-mentioned thick paste, add the dextrin of the amount of processing 60%, spray into 2% hydroxypropyl emthylcellulose ethanol liquid 80m1 and granulate, packing promptly gets.
5. according to the method for preparing of the safe preparation of the said Golden Horse liver of claim 3, it is characterized in that: said capsule prepares like this: get Herba Epimedii and pulverize, use alcohol reflux, filter, reclaim ethanol, filtrating simmer down to thick paste; Herba Dichodrae is pulverized, and carries with alcohol reflux, filters filtrate recycling ethanol and simmer down to thick paste; The Radix Stephaniae Tetrandrae crushed after being dried is a coarse powder, adopts alcohol reflux, and extracting solution reclaims ethanol to there not being the alcohol flavor, under agitation dropwise adds hydrochloric acid; Make PH=5, leave standstill, filter; Transfer PH=9 with strong aqua ammonia, leave standstill the back sucking filtration, the acetone heating for dissolving is used in the dry back of deposition; Adding distil water to solution is little turbid in solution, reclaims acetone and is condensed into thick paste, and is subsequent use; All the other Radix berchemiae lineataes, Herba Verbenae, Herba Patriniae, the Radix Astragali, Radix Paeoniae Rubra and Radix Salviae Miltiorrhizae decocte with water, decocting liquid filters, and filtrating is condensed into clear paste, is chilled to room temperature, adds ethanol, stirs, and leaves standstill, filter, filtrate recycling ethanol, medicinal liquid is condensed into thick paste; Merge above-mentioned thick paste, 60 ℃ of dryings are pulverized, and add the magnesium stearate of the amount of processing 0.5%, and mixing incapsulates, and promptly gets.
6. according to the method for preparing of the safe preparation of the said Golden Horse liver of claim 3, it is characterized in that: said tablet prepares like this: get Herba Epimedii and pulverize, use alcohol reflux, filter, reclaim ethanol, filtrating simmer down to thick paste; Herba Dichodrae is pulverized, and carries with alcohol reflux, filters filtrate recycling ethanol and simmer down to thick paste; The Radix Stephaniae Tetrandrae crushed after being dried is a coarse powder, adopts alcohol reflux, and extracting solution reclaims ethanol to there not being the alcohol flavor, under agitation dropwise adds hydrochloric acid; Make PH=5, leave standstill, filter; Transfer PH=9 with strong aqua ammonia, leave standstill the back sucking filtration, the acetone heating for dissolving is used in the dry back of deposition; Adding distil water to solution is little turbid in solution, reclaims acetone and is condensed into thick paste, and is subsequent use; All the other Radix berchemiae lineataes, Herba Verbenae, Herba Patriniae, the Radix Astragali, Radix Paeoniae Rubra and Radix Salviae Miltiorrhizae decocte with water, decocting liquid filters, and filtrating is condensed into clear paste, is chilled to room temperature, adds ethanol, stirs, and leaves standstill, filter, filtrate recycling ethanol, medicinal liquid is condensed into thick paste; Merge above-mentioned thick paste, add the starch of the amount of processing 32% and 1.3% microcrystalline Cellulose again, granulate, 60 ℃ of dryings, the magnesium stearate of the adding amount of processing 0.1%, mixing, tabletting, the bag film-coat promptly gets.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210324532.1A CN102813752B (en) | 2012-09-05 | 2012-09-05 | Preparation method of creeping dichondra-berchemia lineata-verbena Gantai preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210324532.1A CN102813752B (en) | 2012-09-05 | 2012-09-05 | Preparation method of creeping dichondra-berchemia lineata-verbena Gantai preparation |
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| Publication Number | Publication Date |
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| CN102813752A true CN102813752A (en) | 2012-12-12 |
| CN102813752B CN102813752B (en) | 2014-06-18 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103494957A (en) * | 2013-09-30 | 2014-01-08 | 邬建明 | Preparation method of Jinma Gantai preparation for treating hepatitis |
| CN107569528A (en) * | 2017-10-23 | 2018-01-12 | 贵州拜特制药有限公司 | A kind of pharmaceutical composition for treating hepatitis, pharmaceutical preparation and application and preparation method |
| CN107669819A (en) * | 2017-10-24 | 2018-02-09 | 磐安县派普特生物科技有限公司 | The purposes of microencapsulation tea extract |
| CN107669821A (en) * | 2017-10-23 | 2018-02-09 | 贵州拜特制药有限公司 | Application of the creeping dichondra-berchemia lineata-verbena Gantai preparation in medicine in terms of preparing treatment ischemical reperfusion injury and relevant disease |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1127125A (en) * | 1995-08-04 | 1996-07-24 | 刘三部 | Medicine for hepatitis |
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2012
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1127125A (en) * | 1995-08-04 | 1996-07-24 | 刘三部 | Medicine for hepatitis |
Non-Patent Citations (1)
| Title |
|---|
| 卫生部: "《国家药品中药标准内科肝胆分册》", 31 December 2002 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103494957A (en) * | 2013-09-30 | 2014-01-08 | 邬建明 | Preparation method of Jinma Gantai preparation for treating hepatitis |
| CN103494957B (en) * | 2013-09-30 | 2015-07-08 | 邬建明 | Preparation method of Jinma Gantai preparation for treating hepatitis |
| CN107569528A (en) * | 2017-10-23 | 2018-01-12 | 贵州拜特制药有限公司 | A kind of pharmaceutical composition for treating hepatitis, pharmaceutical preparation and application and preparation method |
| CN107669821A (en) * | 2017-10-23 | 2018-02-09 | 贵州拜特制药有限公司 | Application of the creeping dichondra-berchemia lineata-verbena Gantai preparation in medicine in terms of preparing treatment ischemical reperfusion injury and relevant disease |
| CN107569528B (en) * | 2017-10-23 | 2020-09-29 | 贵州拜特制药有限公司 | Pharmaceutical composition for treating hepatitis, pharmaceutical preparation, application and preparation method |
| CN107669821B (en) * | 2017-10-23 | 2020-12-29 | 贵州拜特制药有限公司 | Application of Jinma Gantai preparation in preparation of medicines for treating ischemia-reperfusion injury and related diseases |
| CN107669819A (en) * | 2017-10-24 | 2018-02-09 | 磐安县派普特生物科技有限公司 | The purposes of microencapsulation tea extract |
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| CN102813752B (en) | 2014-06-18 |
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