CN102807518B - Method for extracting astaxanthin and astaxanthin ester from Euphausia superba by using A beta-8 macroporous resin - Google Patents
Method for extracting astaxanthin and astaxanthin ester from Euphausia superba by using A beta-8 macroporous resin Download PDFInfo
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- CN102807518B CN102807518B CN201210250052.5A CN201210250052A CN102807518B CN 102807518 B CN102807518 B CN 102807518B CN 201210250052 A CN201210250052 A CN 201210250052A CN 102807518 B CN102807518 B CN 102807518B
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- astaxanthin
- macroporous resin
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Abstract
The invention discloses a method for extracting astaxanthin and astaxanthin ester from Euphausia superba by using A beta-8 macroporous resin. The method comprises the following steps of: using Euphausia superba as raw materials, extracting by using ethanol to obtain ethanol extract, regulating the concentration of ethanol of the ethanol extract to 50-70 percent, adding the A beta-8 macroporous resin, agitating for adsorption for 1-3h, separating out red macroporous resin, eluting the astaxanthin and the astaxanthin ester adsorbed by the red macroporous resin by using acetone, and finally evaporating to remove the acetone to obtain red paste, i.e. astaxanthin and astaxanthin ester. The method for extracting astaxanthin and astaxanthin ester from Euphausia superba by using A beta-8 macroporous resin has the advantages that the process is simple and feasible, the method is suitable for industrial production, the full utilization of effective components in Euphausia superba is facilitated, and a technical reference is provided for the extraction and preparation of astaxanthin and astaxanthin ester from Euphausia superba.
Description
Technical field
The present invention relates to the method that a kind of A of utilization β-8 macroporous resin extracts astaxanthin and ester thereof from krill.
Background technology
Krill (Euphausia superba), has another name called large krill or krill, is the krill of one way of life in the waters, the Antarctica of Antarctic Ocean.Live in the mode of trooping, density reaches 10,000～30,000/m sometimes
3.They may be the most successful animal species on the earth, hundred million tons of nearly 5-50 (because methods of evaluation difference has larger difference).Annual approximately 100,000 tons of krill fishery.Mainly fishery country is Japan and Polish.Mainly be used as cooking in Japan, world's elsewhere is as animal-feed and bait.
Natural astaxanthin more than 90% exists with esterified form, more stable.Preparation, storage and the application of large multi-product are all carried out with the form of astaxanthin ester.Krill contains the astaxanthin ester that astaxanthin and astaxanthin are combined with lipid acid, astaxanthin and astaxanthin ester are applied in aquaculture, entering after animal body can be not modified or biochemical conversion and being directly stored in tissue, make skin and the muscle of some hydrocoles occur health and chromatic colour, make feather, skin, pin, the beak of birds, beasts and eggs and fowl present healthy golden yellow and red, be used as at present painted for food of foodstuff additive, fresh-keeping and nutrition.
Astaxanthin is a kind of good antioxidant, aspect the generation of antioxidant cancellation free radical, is playing an important role, and the resistance of oxidation of astaxanthin is than β-carotene and V
emuch higher times.
Astaxanthin has the effect that promotes growth and breeding, can promote the Reproduction of fish, promotes fish-egg fertilization, reduces the mortality ratio of fetal development, accelerates individual growth, increases ripe speed and fecundity, can also improve the laying rate of poultry.
Astaxanthin can strengthen immunity and have antitumous effect, and generation that can enhancing antibody strengthens Immune Function In Animals, also can regulate the activity of portion gene, suppresses the transfer of malignant tumour, has the effect that effectively prevents or slow down cancer.Experimentation on animals shows, and astaxanthin has prophylactic effect to oral carcinoma and bladder cancer.Also there is obvious effect at inhibition liver cancer.By being suppressed to tumorigenic effect, nude mice epidermis description of test astaxanthin is the inhibition to tumor proliferation.
Astaxanthin can effectively prevent by hemato encephalic barrier the damage of amphiblestroid oxidation and photoreceptor cell, can be used for prevention and treatment age-related macular degeneration, improves retinal function.Astaxanthin also have neuroprotective system especially brain and spinal cord be subject to effect of oxidative damage, can effectively treat the central nervous system diseases such as ischemic repetition perfusion injury, Spinal injury, parkinsonism, senile dementia.
Along with progressively going deep into of astaxanthin biological functional study and pharmacological effect test, astaxanthin is widely used in medicine, makeup and high-grade nutrient health care food industries.In makeup, add astaxanthin and can play anti-ageing, sun-proof texts, can keep for a long time painted and be beneficial to skin health for lipstick.
Raw material prepared by astaxanthin has shrimp, crab, shrimp shell, shrimp protein etc., and the hugest with krill resource.And astaxanthin and ester content thereof are abundant.The astaxanthin of krill and ester thereof, phosphatide and oil are three valuable compositions in krill grease, three forms a molten system altogether, how in the process of preparing shrimp sauce, astaxanthin and ester thereof being separated not losing under phosphatide and oil condition, is the problem that must solve in krill grease production process.
Summary of the invention
For above-mentioned prior art, the invention provides a kind of A of utilization β-8 macroporous resin and extract the method for astaxanthin and ester thereof from krill.
The present invention is achieved by the following technical solutions:
Utilize A β-8 macroporous resin from krill, to extract a method for astaxanthin and ester thereof, step is as follows:
(1) obtain krill alcohol extract: get the freezing fresh krill of 1 kilogram, add 1～1.4 liter of 95～100% ethanol (volume fraction), more than stirring extraction 3h, after leaving standstill, pour out extracting solution, 95～100% ethanol of the extracting solution equivalent adding again and pour out repeat to extract twice, extract altogether 3 times, extracting solution merges, filters, and obtains krill alcohol extract; Or: get the dry krill of 1 kilogram, add 6 liter of 95～100% ethanol (volume fraction), more than stirring extraction 3h, after leaving standstill, pour out extracting solution, 95～100% ethanol of the extracting solution equivalent adding again and pour out repeat to extract twice, extract altogether 3 times, extracting solution merges, filters, and obtains krill alcohol extract;
(2) ethanol content of krill alcohol extract is adjusted to 50%～70%(volume fraction), add wherein heavy or 2～3 times of A β-8 macroporous resins that dry shrimp is heavy of 2/5～3/5 chilled shrimp, whip attachment 1～3h, isolates red macroporous resin;
(3) red macroporous resin acetone wash-out 3 times, the twice that the each add-on of acetone is macroporous resin (refers to quality volume multiple, the macroporous resin that is equivalent to 1g redness adds 2ml acetone), macroporous resin is washed till to white, obtain red acetone soln, steam except acetone, obtain red astaxanthin and the paste mixture of ester thereof.
The A of utilization of the present invention β-8 macroporous resin extracts the method for astaxanthin and ester thereof from krill, can adopt fresh krill is raw material, also can adopt dry krill is raw material, by contrast, the present invention is more suitable in the absorption of freezing fresh shrimp ethanol extract, freezing fresh shrimp is moisture general 80%, dry-matter 20%, and macroporous resin need to could adsorb astaxanthin and ester thereof in certain proportion aqueous ethanol liquid.In addition, do raw material with freezing shrimp and can reduce the water consumption that regulates ethanol extract concentration, also reduced the total amount of liquid after absorption astaxanthin and ester thereof simultaneously, time prepared by follow-up phosphatide and triglyceride level, reduce energy burden.
The A of utilization of the present invention β-8 macroporous resin extracts the method for astaxanthin and ester thereof from krill, technological process is simple, be suitable for suitability for industrialized production, be conducive to making full use of of effective constituent in krill, for the extraction preparation of astaxanthin in krill and ester thereof provides Technical Reference.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1:
Freezing krill 500g, adds 600ml 95% ethanol, stirs extraction 3h, pours out 400ml supernatant solution after static, add 400ml 95% ethanol, leaching process is the same again, then repeats this process once, extracts altogether 3 times, finally, by slag extruding, extracting liquid filtering, obtains filtrate 1200ml.Filtrate alcohol concn is adjusted to 50%, adds 200g A β-8 macroporous resin whip attachment 1h, isolate red macroporous resin.
In red macroporous resin, add 400ml acetone, stir wash-out 0.5h, pour out acetone elutriant, repeat this process and wash as white to macroporous resin for twice, red acetone solution is merged, steam except acetone, obtain red paste, i.e. the mixture of astaxanthin and ester thereof.A β-8 macroporous resin can be recycled.
Embodiment 2:
Freezing krill 500g, adds 500ml dehydrated alcohol, stirs extraction 3.5h, after static, pour out 450ml supernatant liquor, then add 450ml dehydrated alcohol, leaching process is the same, repeat this process once, extract altogether 3 times, finally, by slag extruding, extracting solution is merged, be total to obtain filtrate 1400ml, filtrate alcohol concn is adjusted to 70%, adds wherein 300g A β-8 macroporous resin, whip attachment 3h.Red macropore tree is referred to separate, add wherein 600ml acetone, stir wash-out 1h, pour out acetone elutriant, repeat twice of this process to macroporous resin and wash as white.3 acetone elutriants are merged, steam except acetone, obtain red paste, i.e. the mixture of astaxanthin and ester thereof.A β-8 macroporous resin can Reusability.
Embodiment 3:
Freezing krill 500g, adds 700ml 98% ethanol, stirs extraction 3.5h, after static, pour out 500ml supernatant liquor, then add 500ml 98% ethanol, leaching process is the same, repeat this process once, extract altogether 3 times, finally, by slag extruding, extracting solution is merged, be total to obtain filtrate 1500ml, filtrate alcohol concn is adjusted to 60%, adds wherein 250g A β-8 macroporous resin, whip attachment 2h.Red macropore tree is referred to separate, add wherein 500ml acetone, stir wash-out 1h, pour out acetone elutriant, repeat twice of this process to macroporous resin and wash as white.3 acetone elutriants are merged, steam except acetone, obtain red paste, i.e. the mixture of astaxanthin and ester thereof.A β-8 macroporous resin can Reusability.
Claims (1)
1. utilize A β-8 macroporous resin from krill, to extract a method for astaxanthin and ester thereof, it is characterized in that: step is as follows:
(1) obtain krill alcohol extract: get the freezing fresh krill of 1 kilogram, add 1～1.4 liter of 95～100% ethanol, more than stirring extraction 3h, after leaving standstill, pour out extracting solution, 95～100% ethanol of the extracting solution equivalent adding again and pour out repeat to extract twice, extract altogether 3 times, extracting solution merges, filters, and obtains krill alcohol extract; Or: get the dry krill of 1 kilogram, add 6 liter of 95～100% ethanol, more than stirring extraction 3h, after leaving standstill, pour out extracting solution, then add with 95～100% ethanol of the extracting solution equivalent of pouring out and repeat to extract twice, extract altogether 3 times, extracting solution merges, filters, and obtains krill alcohol extract;
(2) ethanol content of krill alcohol extract is adjusted to 50%～70%, adds wherein heavy or 2～3 times of A β-8 macroporous resins that dry shrimp is heavy of 2/5～3/5 chilled shrimp, whip attachment 1～3h, isolates red macroporous resin;
(3) red macroporous resin acetone wash-out 3 times, the twice that the each add-on of acetone is macroporous resin, is washed till white by macroporous resin, obtains red acetone soln, steams except acetone, obtains red astaxanthin and the paste mixture of ester thereof.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210250052.5A CN102807518B (en) | 2012-07-18 | 2012-07-18 | Method for extracting astaxanthin and astaxanthin ester from Euphausia superba by using A beta-8 macroporous resin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210250052.5A CN102807518B (en) | 2012-07-18 | 2012-07-18 | Method for extracting astaxanthin and astaxanthin ester from Euphausia superba by using A beta-8 macroporous resin |
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| CN102807518A CN102807518A (en) | 2012-12-05 |
| CN102807518B true CN102807518B (en) | 2014-08-27 |
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| CN201210250052.5A Expired - Fee Related CN102807518B (en) | 2012-07-18 | 2012-07-18 | Method for extracting astaxanthin and astaxanthin ester from Euphausia superba by using A beta-8 macroporous resin |
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| CN106565577B (en) * | 2016-11-07 | 2018-06-15 | 晨光生物科技集团股份有限公司 | A kind of method for purifying astaxanthin oil |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2214980A1 (en) * | 2001-06-21 | 2004-09-16 | Transucrania, S.A. | Method of processing crustacea in general and, in particular, antarctic krill and the semi-products thus obtained |
| CN102224855A (en) * | 2011-05-13 | 2011-10-26 | 中国海洋大学 | Method for enriching lipid in Antarctic krill |
| CN102334686A (en) * | 2011-07-19 | 2012-02-01 | 中国水产科学研究院东海水产研究所 | Rapid low temperature extraction method for total astaxanthin from frozen Euphausia superba Dana |
-
2012
- 2012-07-18 CN CN201210250052.5A patent/CN102807518B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2214980A1 (en) * | 2001-06-21 | 2004-09-16 | Transucrania, S.A. | Method of processing crustacea in general and, in particular, antarctic krill and the semi-products thus obtained |
| CN102224855A (en) * | 2011-05-13 | 2011-10-26 | 中国海洋大学 | Method for enriching lipid in Antarctic krill |
| CN102334686A (en) * | 2011-07-19 | 2012-02-01 | 中国水产科学研究院东海水产研究所 | Rapid low temperature extraction method for total astaxanthin from frozen Euphausia superba Dana |
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