CN102796188A - Water-soluble fibroin polypeptide food preservative and preparation method - Google Patents
Water-soluble fibroin polypeptide food preservative and preparation method Download PDFInfo
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- CN102796188A CN102796188A CN2012102862742A CN201210286274A CN102796188A CN 102796188 A CN102796188 A CN 102796188A CN 2012102862742 A CN2012102862742 A CN 2012102862742A CN 201210286274 A CN201210286274 A CN 201210286274A CN 102796188 A CN102796188 A CN 102796188A
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- fibroin
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Abstract
The invention discloses a water-soluble fibroin polypeptide food preservative and a preparation method, the method comprises the following steps: degumming silk, weighing silkworm cocoon, immersing and cleaning the silkworm cocoon by clear water for several times, drying and putting in a sodium carbonate aqueous solution with mass concentration of 0.5%, wherein the bath ratio is 1:50(w/w), boiling for 0.5 hours, flushing by tap water, after completing the first degumming, putting the silkworm cocoon after first degumming into the sodium carbonate with mass concentration of 0.5%, wherein the bath ratio is 1:50(w/w), the reaction temperature is 80 DEG C and the process time is 0.5 hour, then cleaning by deionized water, and putting in a furnace at the temperature of 80 DEG C and drying to obtain degumming filament which is fibroin, and degrading the fibroin to obtain the fibroin polypeptide. By observing the bacteriostasis tests of fibroin polypeptide, the bacterium colony diameter in a bacteria (staphylococcus aureus or lactics) medium added with the fibroin polypeptide is less than the bacterium colony diameter of that without fibroin peptide addition, thereby the diameter of the bacterium colony is gradually decreased by increasing the solution concentration of the fibroin peptide.
Description
Technical field
The present invention relates to the bacteriostatic action after the natural silk hydrolysis best approach and the hydrolysis thereof, particularly a kind of water-soluble fibroin polypeptide food sanitas.
Background technology
Food preservatives is the rotten foodstuff additive that use that cause because of microbial reproduction in order to prevent, at present, the food preservatives that allows in the world to use has four or five ten kinds, is mostly artificial synthetic chemical.Because its toxicity (synergistic effect), in recent years, food service industry had the trend that reduces its use gradually both at home and abroad.Comprise that antibacterial peptide (antimicrobial peptides is like nisin, silk peptide etc.) can be the main direction in sanitas market from now at interior natural antiseptic agent, they do not work to eukaryotic cell basically.But except the fibroin polypeptide, present natural based food sanitas costs an arm and a leg, and most food manufacturing enterprise is difficult to bear.China produces silk big country, is used to produce the aboundresources of fibroin polypeptide.Compare with other polypeptide, the fibroin polypeptide also has following nourishing function: (1) reduces SUV in the blood, prevents hypertension and cerebral thrombosis.(2) prevention " senile apoplexy " and dementia.(3) promote secretion of insulin.(4) Antialcoholic liver-protecting.The fibroin polypeptide is a kind of desirable nutritional medicine (nutraceuticals) that is used to realize the health care of food function.In addition, also can research and develop the food fresh keeping wrapping material that silk peptide is processed.
Summary of the invention
The purpose of this invention is to provide a kind of water-soluble fibroin polypeptide food sanitas and preparation method thereof, water-soluble fibroin polypeptide food sanitas preparation method of the present invention may further comprise the steps:
A kind of preparation method of water-soluble fibroin polypeptide food sanitas takes by weighing silk cocoon, with the clear water soaking and washing for several times, puts into mass concentration after drying and be 0.5% yellow soda ash
The aqueous solutionIn, bath raio is 1: 50 (w/w), boils 0.5 hour, washes with tap water; Accomplishing and come unstuck for the first time, is in 0.5% yellow soda ash putting into mass concentration through the silk cocoon that comes unstuck for the first time, and bath raio is 1: 50 (w/w); Temperature of reaction is 80 ℃, and the time is 0.5 hour, uses washed with de-ionized water then; Put into 80 ℃ of oven dry of baking oven at last, obtain degumed silk, i.e. fibroin; Take by weighing fibroin, with 85% phosphoric acid solution dissolving, bath raio is 1: 4 (w/w), and temperature is 130 ℃; In 4 hours reaction times, reaction is carried out neutralization reaction with quicklime after finishing, and control pH value is about 7; Stir and placed 3 hours, use the B suction filtration then, obtain silk peptide solution; The gac that in filtrating, adds filtrating weight 1%, 80 ℃ were stirred 1.5 hours, used the suction filter filtered while hot then, and filter residue is washed 3-4 time, and merging filtrate is removed gac; Pour filtrating into round-bottomed flask, in electric mantle, boil when not sticking with paste, put into 80 ℃ of oven dry of constant temperature oven.
From the bacteriostatic test of fibroin polypeptide, observe colony diameter in bacterium (streptococcus aureus or the milk-acid bacteria) substratum that adds the fibroin polypeptide less than the colony diameter that does not add silk peptide, along with the increase of silk peptide strength of solution, its colony diameter is littler.
Description of drawings
Fig. 1: the staphylococcus aureus culture medium that does not add the fibroin polypeptide;
Fig. 2: add 0.3%
(part by weight, down together)The staphylococcus aureus culture medium of fibroin polypeptide;
Fig. 3: the staphylococcus aureus culture medium that adds 0.6% fibroin polypeptide;
Fig. 4: the staphylococcus aureus culture medium that adds 0.9% fibroin polypeptide
Fig. 5: the milk-acid bacteria substratum that does not add the fibroin polypeptide;
Fig. 6: the milk-acid bacteria substratum that adds 0.3% fibroin polypeptide;
Fig. 7: the milk-acid bacteria substratum that adds 0.6% fibroin polypeptide;
Fig. 8: the milk-acid bacteria substratum that adds 0.9% fibroin polypeptide.
Embodiment
Below in conjunction with specific embodiment, the present invention is elaborated.
Embodiment 1
Take by weighing a certain amount of silk cocoon, with the clear water soaking and washing for several times, putting into mass concentration after drying is in 0.5% yellow soda ash, and bath raio is 1: 50 (w/w); Boiling 0.5 hour, and with the tap water flushing, accomplished and come unstuck for the first time, is in 0.5% yellow soda ash putting into mass concentration through the silk cocoon that comes unstuck for the first time; bath raio is 1: 50 (w/w), and temperature of reaction is 80 ℃, and the time is 0.5 hour, uses washed with de-ionized water then; put into 80 ℃ of oven dry of baking oven at last, obtain degumed silk, i.e. fibroin.
Take by weighing fibroin 2.20g, with 85% phosphoric acid solution dissolving, bath raio is 1: 4 (w/w), and temperature is 130 ℃, 4 hours reaction times.Reaction is carried out neutralization reaction with quicklime after finishing, and the control pH value is about 7, and reaction generates calcium phosphate precipitation, makes the amount of residual phosphate anion drop to minimum.Stir also and placed 3 hours, let abundant reaction, use the B suction filtration then, obtain silk peptide solution.The gac that in filtrating, adds filtrating weight 1%, 80 ℃ were stirred 1.5 hours, used the suction filter filtered while hot then, and filter residue is washed 3-4 time, and merging filtrate is removed gac.Pour filtrating into round-bottomed flask, in electric mantle, boil when not sticking with paste, put into constant temperature oven oven dry (temperature is 80 ℃).Obtain 1.06g exsiccant silk peptide solid through weighing, through calculating, productive rate is about 48.2%.
Embodiment 2
Take by weighing fibroin 2.20g, with 85% phosphoric acid solution dissolving, bath raio is 1: 4 (w/w), and temperature is 130 ℃, 8 hours reaction times.Reaction is carried out neutralization reaction with quicklime after finishing, and the control pH value is about 7, and reaction generates calcium phosphate precipitation, makes the amount of residual phosphate anion drop to minimum.Stir and placed 3 hours, let it fully react, use the B suction filtration then, obtain silk peptide solution.The gac that in filtrating, adds filtrating weight 1%, 80 ℃ were stirred 1.5 hours, used the suction filter filtered while hot then, and filter residue is washed 3-4 time, and merging filtrate is removed gac.Pour filtrating into round-bottomed flask, in electric mantle, boil and be concentrated into when not sticking with paste, put into constant temperature oven oven dry (temperature is 80 ℃).Obtain 1.67g exsiccant silk peptide solid through weighing, through calculating, productive rate is about 75.9%.
Embodiment 3
The bacteriostatic test of fibroin polypeptide
(1) selection of substratum is the most extensive and the prevailing bacterium basic medium of a kind of application with the preparation beef-protein medium, takes by weighing and dissolves in the culture medium prescription ratio, accent
PH 7.4-7.6, packing then, the sterile culture inspection is carried out in sterilization at last, and asepsis growth can use.
(2) dilution spread plate method 1) at first dull and stereotyped, the beef extract-peptone solid medium of having sterilized is cooled to 60 ℃, dull and stereotyped, make substratum be evenly distributed on the petridish bottom, be flat on desktop then, treat condensation.2) preparation bacterial classification diluent; Get a ring bacterial classification inoculation in the beef extract-peptone liquid nutrient medium of 10mL with the transfering loop of calcination, in constant incubator, cultivated 10-12 hour for 37 ℃, therefrom pipette 1mL then and put into sterilized water that fills 99mL and the triangular flask that has granulated glass sphere with pipettor; Jolting; Make bacterial classification and water thorough mixing,, therefrom pipette 100 μ L to being equipped with 900 μ L's with cellular invasion
Sterilized waterIn the centrifuge tube, abundant mixing, and then therefrom pipette 100 μ L and be equipped with 900 μ L's to another
Sterilized waterIn the centrifuge tube, mix all, process 10 by that analogy
-1, 10
-2, 10
-3, 10
-4, 10
-5, 10
-6Different dilution bacterium liquid select 10
-6Be best weaker concn.3) coating pipettes 100 μ L with pipettor and carefully drops in plate culture medium surface central authorities, takes aseptic spreader to be uniformly coated on bacterium liquid on the substratum again.
(3) cultivate the plate culture medium that to be coated with and be inverted, be put in the constant incubator 37 ℃ and cultivated the size of observing colony diameter 24-48 hour.Concrete picture is seen Fig. 1-Fig. 8; From test, observe colony diameter in bacterium (streptococcus aureus or the milk-acid bacteria) substratum that adds the fibroin polypeptide less than the colony diameter that does not add the fibroin polypeptide; Along with the increase of silk peptide strength of solution, its colony diameter is littler.
Should be understood that, concerning those of ordinary skills, can improve or conversion, and all these improvement and conversion all should belong to the protection domain of accompanying claims of the present invention according to above-mentioned explanation.
Claims (2)
1. the preparation method of a water-soluble fibroin polypeptide food sanitas is characterized in that,
Silk comes unstuck:Take by weighing silk cocoon, with the clear water soaking and washing for several times, put into mass concentration after drying and be 0.5% yellow soda ash
The aqueous solutionIn, bath raio is 1: 50 (w/w), boils 0.5 hour, washes with tap water; Accomplishing and come unstuck for the first time, is in 0.5% yellow soda ash putting into mass concentration through the silk cocoon that comes unstuck for the first time, and bath raio is 1: 50 (w/w); Temperature of reaction is 80 ℃, and the time is 0.5 hour, uses washed with de-ionized water then; Put into 80 ℃ of oven dry of baking oven at last, obtain degumed silk, i.e. fibroin; Take by weighing fibroin, with 85% phosphoric acid solution dissolving, bath raio is 1: 4 (w/w), and temperature is 130 ℃; In 4 hours reaction times, reaction is carried out neutralization reaction with quicklime after finishing, and control pH value is about 7; Stir and placed 3 hours, use the B suction filtration then, obtain silk peptide solution; The gac that in filtrating, adds filtrating weight 1%, 80 ℃ were stirred 1.5 hours, used the suction filter filtered while hot then, and filter residue is washed 3-4 time, and merging filtrate is removed gac; Pour filtrating into round-bottomed flask, in electric mantle, boil when not sticking with paste, put into 80 ℃ of oven dry of constant temperature oven.
2. the water-soluble fibroin polypeptide food sanitas that method according to claim 1 prepares.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104987374A (en) * | 2015-07-28 | 2015-10-21 | 苏州普罗达生物科技有限公司 | Female silkworm silk fibroin polypeptide, and preparation method and application thereof |
| CN106231919A (en) * | 2014-03-07 | 2016-12-14 | 塔夫茨大学 | The preservation of perishable farm products based on biopolymer |
| CN107307062A (en) * | 2017-08-08 | 2017-11-03 | 福建农林大学 | A kind of konjaku glucomannan shrimp antistaling agent and preparation method thereof |
| CN108309871A (en) * | 2018-05-04 | 2018-07-24 | 杨恒智 | A kind of crease-resistant moisturizing water lock Essence and preparation method thereof |
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| CN1415626A (en) * | 2002-10-31 | 2003-05-07 | 辽宁柞蚕丝绸科学研究院有限责任公司 | Method for preparing peptide of tussur silk |
| CN101965982A (en) * | 2010-10-26 | 2011-02-09 | 曹新志 | Method for preparing beverage from silk peptide |
| CN102172257A (en) * | 2011-01-20 | 2011-09-07 | 肖仲君 | Method for making superfine silk peptide powder |
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| CN1415626A (en) * | 2002-10-31 | 2003-05-07 | 辽宁柞蚕丝绸科学研究院有限责任公司 | Method for preparing peptide of tussur silk |
| CN101965982A (en) * | 2010-10-26 | 2011-02-09 | 曹新志 | Method for preparing beverage from silk peptide |
| CN102172257A (en) * | 2011-01-20 | 2011-09-07 | 肖仲君 | Method for making superfine silk peptide powder |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106231919A (en) * | 2014-03-07 | 2016-12-14 | 塔夫茨大学 | The preservation of perishable farm products based on biopolymer |
| CN106231919B (en) * | 2014-03-07 | 2020-06-19 | 塔夫茨大学 | Preservation of perishable products based on biopolymers |
| US11147282B2 (en) | 2014-03-07 | 2021-10-19 | Tufts University | Biopolymer-based preservation of perishable products |
| CN104987374A (en) * | 2015-07-28 | 2015-10-21 | 苏州普罗达生物科技有限公司 | Female silkworm silk fibroin polypeptide, and preparation method and application thereof |
| CN107307062A (en) * | 2017-08-08 | 2017-11-03 | 福建农林大学 | A kind of konjaku glucomannan shrimp antistaling agent and preparation method thereof |
| CN107307062B (en) * | 2017-08-08 | 2020-06-16 | 福建农林大学 | Konjac glucomannan shrimp preservative and preparation method thereof |
| CN108309871A (en) * | 2018-05-04 | 2018-07-24 | 杨恒智 | A kind of crease-resistant moisturizing water lock Essence and preparation method thereof |
| CN108309871B (en) * | 2018-05-04 | 2021-02-23 | 广东赛尔生物科技有限公司 | Anti-wrinkle moisturizing and water-locking essence and preparation method thereof |
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Application publication date: 20121128 |