CN107027810A - A kind of nisin cyclodextrin inclusion compound and preparation method thereof - Google Patents
A kind of nisin cyclodextrin inclusion compound and preparation method thereof Download PDFInfo
- Publication number
- CN107027810A CN107027810A CN201710195893.3A CN201710195893A CN107027810A CN 107027810 A CN107027810 A CN 107027810A CN 201710195893 A CN201710195893 A CN 201710195893A CN 107027810 A CN107027810 A CN 107027810A
- Authority
- CN
- China
- Prior art keywords
- nisin
- inclusion compound
- concentration
- cyclodextrin
- mixed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3562—Sugars; Derivatives thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Nutrition Science (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Polymers & Plastics (AREA)
- Dentistry (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Medicinal Preparation (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
Abstract
The invention provides a kind of nisin cyclodextrin inclusion compound and preparation method thereof, by cyclodextrin in deionized water, nisin powder is dissolved in pH3.0 aqueous hydrochloric acid solution, two kinds of solution are mixed, pH of mixed is adjusted to 6.5 ~ 7.5, at 50 70 DEG C, stirred 12 hours, continue to stir 12 hours at room temperature, spray drying obtains powdered inclusion compound after stirring is finished.Inclusion compound obtained by the present invention improves nisin applied to the stability in liquid food and heating process, reaches the effectively sterilization in more than two weeks in liquid food, can meet the requirement that liquid food cold chain sells shelf life.Meanwhile, heat endurance is improved, influence during reduction food heat sterilization to antiseptic effect.
Description
Technical field
The invention belongs to food preservative technical field, and in particular to a kind of nisin cyclodextrin inclusion compound and its preparation
Method.
Background technology
Nisin is the high security food preservative that a kind of global range majority state approved is used, and China has criticized
Standard is used for many years, the extensive use in various food systems.But easily degrade, make when nisin is used in a liquid
Its bactericidal effect declines very fast, it is difficult to meet the requirement for anticorrosion of more than two weeks shelf life of liquid food.Meanwhile, nisin
Heat endurance it is also not high enough, the heat sterilization of food is larger to its activity influence.Liquid is applied to improve nisin
Stability during food and heat sterilization, domestic and international scientific and technical personnel have carried out substantial amounts of research work, have developed a series of breasts
The micro-capsule of acid streptococci element, hence it is evident that improve its stability in liquid food and heating process.But develop both at home and abroad at present
Nisin micro-capsule there is serious defect, be exactly in first three day for liquid food, its burst size compared with
Small, bactericidal effect is not enough to anti-corrosion, although the sterilization duration was up to more than 15 days later, but early stage antiseptic effect shortcoming makes food
Quality is impacted, it is difficult to practical application.
The content of the invention
The invention provides nisin cyclodextrin inclusion compound, the inclusion compound improves nisin applied to liquid
Stability in body food and heating process, reaches the effectively sterilization in more than two weeks in liquid food, can meet liquid food cold
The requirement of chain pin Goodsshelves phase.Meanwhile, heat endurance is improved, influence when reducing food heat sterilization to antiseptic effect.
The technical proposal for solving the technical problem of the invention is：
A kind of nisin cyclodextrin inclusion compound, the inclusion compound is made by following methods：By cyclodextrin in go from
In sub- water, nisin powder is dissolved in pH3.0 aqueous hydrochloric acid solution, and two kinds of solution are mixed, pH of mixed is adjusted
To 6.5 ~ 7.5, at 50-70 DEG C, stirring 1-2 hours, continue to stir 1-2 hours at room temperature, stirring is spray-dried after finishing
To powdered inclusion compound.
Invention also provides a kind of preparation method of nisin cyclodextrin inclusion compound, by cyclodextrin in
In deionized water, nisin powder is dissolved in pH3.0 aqueous hydrochloric acid solution, two kinds of solution is mixed, by mixed liquor
PH is adjusted to 6.5 ~ 7.5, at 50-70 DEG C, stirring 1-2 hours, continues to stir 1-2 hours at room temperature, stirring is sprayed dry after finishing
It is dry to obtain powdered inclusion compound.
Preferably, the cyclodextrin and the mass ratio of deionized water are 1:5-10.
Preferably, the addition of the nisin powder is 33-100g/L aqueous hydrochloric acid solutions.
Preferably, the technological parameter of the spray drying is 100 ~ 120 DEG C of EAT, atomizing pressure 0.1 ~
0.2MPa, sample introduction 0.2 ~ 0.4L/h of speed, the m of intake 0.25 ~ 0.603/min。
Biuret method surveys streptococcus lactis cellulose content：
Prepare biuret reagent：Weigh 6 g NaOH and add beaker, measure 25 mL deionized waters and add in beaker and dissolve NaOH,
It is diluted to 6g/L standby.Weigh 0.75 gram of copper sulphate（CuSO4·5H2O）It is dissolved in 500mL deionized water, adds 2.25g wine
Stone acid potassium sodium（KNaC4H4O6·4H2O）, and KI 1.5g are added, after being completely dissolved, 6g/L NaOH are added while stirring
Solution 25mL, and 250mL is settled to deionized water, it is positioned over sealing preserve in plastic bottle.
Make standard curve：Compound concentration for 30mg/mL nisin titer and filter, take titer 0,
0.2nd, 0. 4,0. 6,0. 8,1.0mL is mended to 1mL in test tube, then with deionized water, is added 4mL biuret reagents and is mixed, and
After 30min is stood, in testing its absorbance under 540nm wavelength, blank is the solution for being not added with nisin, with extinction
Degree draws standard curve to the mapping of nisin solution concentration.The concentration of nisin solution and the relation of absorbance
Curve is shown in 5-30mg/mL concentration ranges, and good linear relationship, standard curve side is presented in absorbance and the concentration of solution
Journey is A=0.0142C+0.0033；R2=0.9998 (n=5).
Sample detection：The nisin sample solution for drawing 1mL is put into test tube, is added 4mL biuret reagents, is filled
Divide well mixed, place and survey its absorbance after 30min at 540nm.Solution is obtained finally according to the regression equation of standard curve
The content of middle nisin.
The calculating of inclusion rate：Before inclusion stirring finishes spray drying, sample liquid 10 ml for taking that stirring finishes, 10000 turns/
Minute centrifugation 10 minutes, takes the content of nisin in the ml of centrifuge tube supernatant liquor 5, detection supernatant liquor.
Inclusion rate=(W-V*C)/W*100%, the nisin amount put into when W is inclusion, V is that clathrate process spraying is dry
Cumulative volume before dry, C is the concentration of nisin in filtrate.
The detection of nisin cyclodextrin inclusion compound bactericidal effect：Test strain：Staphylococcus aureus
(Staphylococcus aureus), Escherichia coli (Escherichia coli), with Escherichia coli, staphylococcus aureus
Use nutrient agar（Hangzhou microorganism reagent Co., Ltd produces）The Shaking culture in 250mL conical flasks, controls cell
Number is 4.5 × 106CFU/mL or so, is used as sterilization test bacterium solution.
Sterilization test：Take inclusion compound to be dissolved in deionized water, be configured to nisin up to 10mg/mL, 20mg/mL,
The bactericidal liquid of 30mg/mL concentration, takes 0.2mL bactericidal liquids to be well mixed with diluting 100 times of 9.8mL bacterium solutions, nisin
Concentration is 200 μ g/mL, 400 μ g/mL, 600 μ g/mL.It is 5.5 to adjust pH, takes out 0.1ml spread plates, and each concentration bactericidal liquid is applied
3 flat boards, are inverted in 30 DEG C of culture 24h of constant temperature in constant incubator.Observe and record the bacterium colony number of each flat board, same concentration
Flat-plate bacterial colony number average.
Nisin bactericidal effect contrast test：Extracting lactic acid streptostacin be configured to 10mg/mL, 20mg/mL,
The bactericidal liquid of 30mg/mL concentration, takes 0.2 mL to be well mixed with diluting 100 times of 9.8mL bacterium solutions, and it is 5.5 to adjust pH, is taken out
0.1ml spread plates, each concentration is coated with 3 flat boards, and 30 DEG C of constant temperature is inverted culture 24h in incubator.Observe and record
The bacterium colony number of each flat board, the bacterium colony number of same concentration flat board is averaged.
Blank control test：0.2mL deionized waters are added in the bacterium solution that 9.8mL dilutes 100 times, tune pH is after mixing
5.5, therefrom take 0.1mL to be coated on corresponding solid plate, after coating is uniform, 30 DEG C of constant temperature is inverted culture in incubator
24h.The bacterium colony number of each flat board is observed and records, the bacterium colony number of same concentration flat board is averaged.
The sterilizing rate of various concentrations bactericidal liquid is calculated according to sterilizing rate formula：
Sterilizing rate=（Blank test clump count-plus bactericidal liquid experiment clump count）/ blank test clump count * 100%
Nisin cyclodextrin inclusion compound Continuous sterilization effect test：
1. the control of 50mL concentration is prepared 4.5 × 106CFU/mL bacterium solution.
2. inclusion compound is configured to the bactericidal liquid that nisin concentration reaches 10mg/mL, 20mg/mL, 30mg/mL, by 1mL
Bactericidal liquid adds 49mL bacterium solutions, then nisin concentration is 200 μ g/mL, 400 μ g/mL, 600 μ g/mL.30 DEG C of progress,
1mL is taken after 200 r/min shaking table cultures, culture 24h, progress is down flat plate culture.1mL is therefrom taken to carry out being down flat plate training daily later
Support, continuous sampling culture 20 days, Continuous sterilization effect of the observation inclusion compound bactericidal liquid to Escherichia coli and staphylococcus aureus.
Contrast experiment is set, the cyclodextrin of same concentration is ibid operated with nisin mixture, contrast is seen
Examine Continuous sterilization effect.With without any bactericidal liquid for blank control.
Sterilizing rate is calculated according to sterilizing rate computational methods, the Continuous sterilization of inclusion compound is judged according to the situation of change of sterilizing rate
Effect.
Nisin cyclodextrin inclusion compound heat stability test：Inclusion compound is configured into nisin concentration is
1.0mg/mL and 5.0mg/mL solution, free lactic acid streptostacin is configured to same concentrations solution, and 121 DEG C of high temperature are carried out respectively
Autoclaving 5min, 10min.Then investigate through the inclusion compound and free lactic acid streptostacin after 121 DEG C of autoclave sterilizations
Bactericidal effect.
Beneficial effects of the present invention are：
1st, in the nisin cyclodextrin inclusion compound that the present invention is developed, the water for being dissolvable in water nature pH, after dissolving
Stronger bactericidal effect is reached, when the concentration of nisin reaches 200 μ g/ml and the above, the sterilization of inclusion complex in solution
Effect is identical with the free lactic acid streptostacin of same concentration.When the concentration of nisin reach 400 μ g/ml and more than
When, inclusion compound in liquid food the bactericidal effect duration up to 16 ~ 18 days, compared with free lactic acid streptostacin in liquid food
In sterilizing time be obviously prolonged.
2nd, through 121 DEG C of heat sterilization 10min, the 5.0mg/mL solution of free lactic acid streptostacin is without bactericidal effect, sheet
The heat endurance of the developed nisin cyclodextrin inclusion compound inclusion compound of invention is improved, the nisin that inclusion compound is prepared
Rhzomorph concentration is 1.0mg/mL solution, through 121 DEG C of heat sterilization 10min, still with 60% or so bactericidal effect.
Embodiment
The invention will be further described below, but technical parameter involved in scheme can not be interpreted as to the limit to the present invention
System.
Embodiment 1
The food-grade cyclodextrin of 600 grams of Zibo Qian Hui bio tech ltd production is weighed in 5 liters of deionized waters, is claimed
Take the nisin of the biological Co., Ltd's productions of 300 grams of Tianjin Kang Yi（Purity 90%）It is dissolved in 3 liters of pH3.0 hydrochloric acid water
In solution, both are mixed, stirred, pH of mixed is adjusted to 7.0 with the sodium hydrate aqueous solution of 2 mol/L concentration, then protect
70 DEG C are held, is stirred 2 hours, continues to stir 2 hours at room temperature.Stirring is finished, with 110 DEG C of EAT, atomizing pressure
0.15MPa, sample introduction speed 0.25L/L, the m of intake 0.503/ min process conditions spray dried products, obtain inclusion compound 854
Gram.
The ml of sample liquid 10 that stirring is finished is taken, 10000 revs/min centrifuge 10 minutes, take the ml of centrifuge tube supernatant liquor 5, with
The amount of nisin, calculates the total amount not included in biuret spectrophotometry clear liquid, according to the lactic acid of addition
Streptostacin quality, it is 65% to calculate inclusion rate.
Inclusion compound is by the bactericidal effect assay method drafted, Continuous sterilization effect detection method, heat stability test method,
Made comparisons with free lactic acid streptostacin.After measured, when inclusion compound is dissolved in water, its nisin is up to 200 μ g/ml concentration
When, there is identical sterilizing rate with same concentrations free lactic acid streptostacin；The μ g/ml of free lactic acid streptostacin concentration 400, hold
Continuous bactericidal effect was up to 7 days, and inclusion complex in solution nisin concentration is up to 400 μ g/ml, and Continuous sterilization effect was up to 16 days；
Inclusion compound is made into nisin concentration 1.0mg/mL and 5.0mg/mL sample liquid, free lactic acid streptostacin is prepared identical
Two kinds of sample liquids of concentration, through 121 DEG C of heat sterilizations 5 minutes, after testing, the free lactic acid streptostacin liquid of two kinds of concentration was without killing
Bacterium effect, and inclusion compound concentration 1.0mg/mL sample liquid remains 65% sterilizing rate, concentration 5.0mg/mL sample liquid is remained
93% sterilizing rate；Through 121 DEG C of heat sterilizations 10 minutes, after testing, the free lactic acid streptostacin liquid of two kinds of concentration was without sterilization
Effect, and inclusion compound concentration 1.0mg/mL sample liquid remains 60% sterilizing rate, concentration 5.0mg/mL sample liquid remains 92%
Sterilizing rate.
Embodiment 2
600 grams of sea salt six and the food-grade cyclodextrin of starch Chemical Industry Co., Ltd production are in 4 liters of deionized waters, 200 grams
The Nisin of Xian Bao Bioisystech Co., Ltd of Wuhan City production（Purity 80%）, will in the aqueous hydrochloric acid solution for being dissolved in 3 liters of pH3.0
Both mixing, are stirred, and pH of mixed is adjusted to 7.5 with the sodium hydrate aqueous solution of 2 mol/L concentration, in the case where being kept for 60 DEG C,
Stirring 1.5 hours, continues to stir 1.5 hours at room temperature.Stirring is finished, with 115 DEG C of EAT, atomizing pressure 0.2MPa,
The L/h of sample introduction speed 0.3, the m of intake 0.403/ min process conditions spray dried products, obtain 751 grams of inclusion compound.
The ml of sample liquid 10 that stirring is finished is taken, 10000 revs/min centrifuge 10 minutes, take the ml of centrifuge tube supernatant liquor 5, with
The amount of nisin, calculates the total amount not included in biuret spectrophotometry clear liquid, according to the lactic acid of addition
Streptostacin quality, it is 66% to calculate inclusion rate.
Inclusion compound is by the bactericidal effect assay method drafted, Continuous sterilization effect detection method, heat stability test method,
Made comparisons with free lactic acid streptostacin.After measured, when inclusion compound is dissolved in water, its nisin is up to 200 μ g/ml concentration
When, there is identical sterilizing rate with same concentrations free lactic acid streptostacin；The μ g/ml of free lactic acid streptostacin concentration 400, hold
Continuous bactericidal effect was up to 7 days, and inclusion complex in solution nisin concentration is up to 400 μ g/ml, and Continuous sterilization effect was up to 17 days；
Inclusion compound is made into nisin concentration 1.0mg/mL and 5.0mg/mL sample liquid, free lactic acid streptostacin is prepared identical
Two kinds of sample liquids of concentration, through 121 DEG C of heat sterilizations 5 minutes, after testing, the free lactic acid streptostacin liquid of two kinds of concentration was without killing
Bacterium effect, and inclusion compound concentration 1.0mg/mL sample liquid remains 64% sterilizing rate, concentration 5.0mg/mL sample liquid is remained
94% sterilizing rate；Through 121 DEG C of heat sterilizations 10 minutes, after testing, the free lactic acid streptostacin liquid of two kinds of concentration was without sterilization
Effect, and inclusion compound concentration 1.0mg/mL sample liquid remains 61% sterilizing rate, concentration 5.0mg/mL sample liquid remains 93%
Sterilizing rate.
Embodiment 3
The food-grade cyclodextrin of 600 grams of Wuxi City Run Xing starch-based products Co., Ltd production is in 3 liters of deionized waters, and 100
The Nisin of the really prosperous chemical industry food additives Co., Ltd production in gram Zhengzhou（Purity 95%）The hydrochloric acid for being dissolved in 3 liters of pH3.0 is water-soluble
In liquid, both are mixed, stirred, adjusts pH of mixed to 6.5 with the sodium hydrate aqueous solution of 2 mol/L concentration, is keeping
At 50 DEG C, stir 1 hour, continue to stir 1 hour at room temperature.Stirring is finished, with 100 DEG C of EAT, atomizing pressure
0.1MPa, the L/h of sample introduction speed 0.2, the m of intake 0.253/ min process conditions spray dried products, obtain inclusion compound 653
Gram.
The ml of sample liquid 10 that stirring is finished is taken, 10000 revs/min centrifuge 10 minutes, take the ml of centrifuge tube supernatant liquor 5, with
The amount of nisin, calculates the total amount not included in biuret spectrophotometry clear liquid, according to the lactic acid of addition
Streptostacin quality, it is 67% to calculate inclusion rate.
Inclusion compound is by the bactericidal effect assay method drafted, Continuous sterilization effect detection method, heat stability test method,
Made comparisons with free lactic acid streptostacin.After measured, when inclusion compound is dissolved in water, its nisin is up to 200 μ g/ml concentration
When, there is identical sterilizing rate with same concentrations free lactic acid streptostacin；The μ g/ml of free lactic acid streptostacin concentration 400, hold
Continuous bactericidal effect was up to 7 days, and inclusion complex in solution nisin concentration is up to 400 μ g/ml, and Continuous sterilization effect was up to 18 days；
Inclusion compound is made into nisin concentration 1.0mg/mL and 5.0mg/mL sample liquid, free lactic acid streptostacin is prepared identical
Two kinds of sample liquids of concentration, through 121 DEG C of heat sterilizations 5 minutes, after testing, the free lactic acid streptostacin liquid of two kinds of concentration was without killing
Bacterium effect, and inclusion compound concentration 1.0mg/mL sample liquid remains 66% sterilizing rate, concentration 5.0mg/mL sample liquid is remained
95% sterilizing rate；Through 121 DEG C of heat sterilizations 10 minutes, after testing, the free lactic acid streptostacin liquid of two kinds of concentration was without sterilization
Effect, and inclusion compound concentration 1.0mg/mL sample liquid remains 59% sterilizing rate, concentration 5.0mg/mL sample liquid remains 93%
Sterilizing rate.
Embodiment 4
The food-grade cyclodextrin of 600 grams of Chengdu Kai Hua Food Co., Ltd production is in 6 liters of deionized waters, 300 grams of Tianjin
The Nisin of Kang Yi biologies Engineering Co., Ltd production（Purity 80%）In the aqueous hydrochloric acid solution for being dissolved in 3 liters of pH3.0, both are mixed
Close, stir, pH of mixed is adjusted to 7.0, in the case where being kept for 70 DEG C, stirring 2 with the sodium hydrate aqueous solution of 2 mol/L concentration
Hour, continue to stir 2 hours at room temperature.Stirring is finished, with 120 DEG C of EAT, atomizing pressure 0.15MPa, sample introduction speed
0.4 L/h, the m of intake 0.603/ min process conditions spray dried products, obtain 856 grams of inclusion compound.
The ml of sample liquid 10 that stirring is finished is taken, 10000 revs/min centrifuge 10 minutes, take the ml of centrifuge tube supernatant liquor 5, with
The amount of nisin, calculates the total amount not included in biuret spectrophotometry clear liquid, according to the lactic acid of addition
Streptostacin quality, it is 66% to calculate inclusion rate.
Inclusion compound is by the bactericidal effect assay method drafted, Continuous sterilization effect detection method, heat stability test method,
Made comparisons with free lactic acid streptostacin.After measured, when inclusion compound is dissolved in water, its nisin is up to 200 μ g/ml concentration
When, there is identical sterilizing rate with same concentrations free lactic acid streptostacin；The μ g/ml of free lactic acid streptostacin concentration 400, hold
Continuous bactericidal effect was up to 7 days, and inclusion complex in solution nisin concentration is up to 400 μ g/ml, and Continuous sterilization effect was up to 17 days；
Inclusion compound is made into nisin concentration 1.0mg/mL and 5.0mg/mL sample liquid, free lactic acid streptostacin is prepared identical
Two kinds of sample liquids of concentration, through 121 DEG C of heat sterilizations 5 minutes, after testing, the free lactic acid streptostacin liquid of two kinds of concentration was without killing
Bacterium effect, and inclusion compound concentration 1.0mg/mL sample liquid remains 65% sterilizing rate, concentration 5.0mg/mL sample liquid is remained
94% sterilizing rate；Through 121 DEG C of heat sterilizations 10 minutes, after testing, the free lactic acid streptostacin liquid of two kinds of concentration was without sterilization
Effect, and inclusion compound concentration 1.0mg/mL sample liquid remains 60% sterilizing rate, concentration 5.0mg/mL sample liquid remains 93%
Sterilizing rate.
The preferred embodiments of the invention are only listed above, and protection scope of the present invention is not restricted to this, this area
Any change that technical staff is made within the scope of the invention as claimed is each fallen within the scope of the present invention.
Claims (5)
1. a kind of nisin cyclodextrin inclusion compound, it is characterised in that the nisin cyclodextrin inclusion compound by
Following methods are made：By cyclodextrin in deionized water, nisin powder is dissolved in pH3.0 aqueous hydrochloric acid solution
In, two kinds of solution are mixed, pH of mixed is adjusted to 6.5 ~ 7.5, at 50-70 DEG C, stirs 1-2 hours, continues to stir at room temperature
Mix 1-2 hours, spray drying obtains powdered inclusion compound after stirring is finished.
2. a kind of preparation method of nisin cyclodextrin inclusion compound, it is characterised in that by cyclodextrin in deionization
In water, nisin powder is dissolved in pH3.0 aqueous hydrochloric acid solution, and two kinds of solution are mixed, pH of mixed is adjusted to
6.5 ~ 7.5, at 50-70 DEG C, stirring 1-2 hours, continue to stir 1-2 hours at room temperature, stirring is spray-dried after finishing and obtained
Powdered inclusion compound.
3. the preparation method of nisin cyclodextrin inclusion compound as claimed in claim 1, it is characterised in that the ring paste
The mass ratio of essence and deionized water is 1:5-10.
4. the preparation method of nisin cyclodextrin inclusion compound as claimed in claim 1, it is characterised in that the lactic acid
The addition of streptostacin powder is 33-100g/L aqueous hydrochloric acid solutions.
5. the preparation method of nisin cyclodextrin inclusion compound as claimed in claim 1, it is characterised in that the spraying
Dry technological parameter is 100 ~ 120 DEG C of EAT, 0.1 ~ 0.2MPa of atomizing pressure, sample introduction 0.2 ~ 0.4L/h of speed, air intake
Measure 0.25 ~ 0.60 m3/min。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710195893.3A CN107027810A (en) | 2017-03-29 | 2017-03-29 | A kind of nisin cyclodextrin inclusion compound and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710195893.3A CN107027810A (en) | 2017-03-29 | 2017-03-29 | A kind of nisin cyclodextrin inclusion compound and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107027810A true CN107027810A (en) | 2017-08-11 |
Family
ID=59534686
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710195893.3A Pending CN107027810A (en) | 2017-03-29 | 2017-03-29 | A kind of nisin cyclodextrin inclusion compound and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107027810A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109627300A (en) * | 2019-02-18 | 2019-04-16 | 浙江新银象生物工程有限公司 | The exploitation of Nisin solution stabilizer and application |
| CN110506741A (en) * | 2019-09-20 | 2019-11-29 | 天津科技大学 | A kind of compound antibacterial material of novel nano and preparation method thereof and purposes |
| CN115624127A (en) * | 2022-09-29 | 2023-01-20 | 浙江工业大学 | Nisin starch adsorbent and preparation method and application thereof |
| CN117441878A (en) * | 2023-12-07 | 2024-01-26 | 北方民族大学 | Modified starch emulsifier and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1068808A1 (en) * | 1989-02-21 | 2001-01-17 | Viskase Corporation | Antimicrobial compositions and method for surface treatment of foodstuffs |
| CN105265994A (en) * | 2015-10-26 | 2016-01-27 | 天津科技大学 | Method effectively improving heat stability of Nisin |
-
2017
- 2017-03-29 CN CN201710195893.3A patent/CN107027810A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1068808A1 (en) * | 1989-02-21 | 2001-01-17 | Viskase Corporation | Antimicrobial compositions and method for surface treatment of foodstuffs |
| CN105265994A (en) * | 2015-10-26 | 2016-01-27 | 天津科技大学 | Method effectively improving heat stability of Nisin |
Non-Patent Citations (1)
| Title |
|---|
| 宋博凡: "乳酸链球菌素微球的制备与杀菌效果研究", 《浙江工业大学硕士学位论文》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109627300A (en) * | 2019-02-18 | 2019-04-16 | 浙江新银象生物工程有限公司 | The exploitation of Nisin solution stabilizer and application |
| CN109627300B (en) * | 2019-02-18 | 2022-08-05 | 浙江新银象生物工程有限公司 | Nisin solution stabilizer development and application |
| CN110506741A (en) * | 2019-09-20 | 2019-11-29 | 天津科技大学 | A kind of compound antibacterial material of novel nano and preparation method thereof and purposes |
| CN110506741B (en) * | 2019-09-20 | 2021-09-21 | 天津科技大学 | Novel nano composite antibacterial material and preparation method and application thereof |
| CN115624127A (en) * | 2022-09-29 | 2023-01-20 | 浙江工业大学 | Nisin starch adsorbent and preparation method and application thereof |
| CN117441878A (en) * | 2023-12-07 | 2024-01-26 | 北方民族大学 | Modified starch emulsifier and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107027810A (en) | A kind of nisin cyclodextrin inclusion compound and preparation method thereof | |
| CN102816440B (en) | Isolated soy protein-nano silver antibacterial composite membrane and preparation method thereof | |
| CN104222265A (en) | Complex preservative and application thereof in fruit and vegetable preservation | |
| CN104164414B (en) | Enzyme/graphene oxide functional form hybridized nanometer compound and preparation method thereof | |
| CN111978555A (en) | Ternary metal organic framework sterilization material and preparation method and application thereof | |
| CN111039328A (en) | Composite metal nano material and preparation and application thereof | |
| CN102153674B (en) | P-aminobenzoate chitosan ester and preparation method thereof | |
| CN107484771A (en) | A kind of 84 thimerosals and preparation method thereof | |
| CN103918714A (en) | Culturing medium antiseptic for Anoectochilus roxburghii (Wall.) Lindl tissue culture, and its preparation method | |
| CN103642070A (en) | Antibiotic composite membrane of nano-silk fibroin-chitosan-nano-silver | |
| CN101263924A (en) | Natural food preservative substance formula and preparation | |
| CN106982838A (en) | A kind of antibiosis additive of packaging material for food | |
| CN109678733A (en) | A kind of quaternary ammonium salt abietyl cationic surfactant and the preparation method and application thereof | |
| CN103815520B (en) | Cross-linked chitosan food preservative, and preparation method and application thereof | |
| CN109221102A (en) | A kind of antibacterial new material | |
| JPH0381209A (en) | Antimicrobial agent and film | |
| JP5160157B2 (en) | Microbial culture medium and microbiological testing instrument for oily samples | |
| CN102757902A (en) | Filamentous fungus culture medium, method for preparing same, and method for culturing filamentous fungi utilizing culture medium | |
| CN108128857B (en) | Composite organic acidifier for keeping drinking water of livestock clean and application thereof | |
| CN104222266A (en) | Gamma-polyglutamic acid-Cu (II) preservative and application thereof to preservation of fruits and vegetables | |
| CN102827069B (en) | Method for preparing two organic compounds by using chlorogenic acid as raw material and application thereof | |
| CN107177045A (en) | A kind of collagen-lysozyme antibiotic film and preparation method thereof | |
| CN107177046A (en) | A kind of collagen-Herba Origani Vulgaris quintessence oil antibacterial film and preparation method thereof | |
| CN105200116A (en) | Method of quickly measuring content of Paenibacillus polymyxa in compound microbial agent | |
| CN101878779B (en) | Disinfectant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170811 |