CN102660595A - Preparation method of corn steep liquor - Google Patents
Preparation method of corn steep liquor Download PDFInfo
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- CN102660595A CN102660595A CN2011104528304A CN201110452830A CN102660595A CN 102660595 A CN102660595 A CN 102660595A CN 2011104528304 A CN2011104528304 A CN 2011104528304A CN 201110452830 A CN201110452830 A CN 201110452830A CN 102660595 A CN102660595 A CN 102660595A
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- steep liquor
- corn steep
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- filtration
- micro
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- 240000008042 Zea mays Species 0.000 title claims abstract description 47
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 47
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 47
- 235000005822 corn Nutrition 0.000 title claims abstract description 47
- 238000002360 preparation method Methods 0.000 title claims abstract description 41
- 238000001471 micro-filtration Methods 0.000 claims abstract description 34
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 239000012528 membrane Substances 0.000 claims abstract description 18
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 62
- 239000012530 fluid Substances 0.000 claims description 17
- 238000000855 fermentation Methods 0.000 abstract description 25
- 230000004151 fermentation Effects 0.000 abstract description 25
- 150000001413 amino acids Chemical class 0.000 abstract description 21
- 239000002253 acid Substances 0.000 abstract description 16
- 230000001954 sterilising effect Effects 0.000 abstract description 10
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 9
- 238000011109 contamination Methods 0.000 abstract 3
- 244000005700 microbiome Species 0.000 abstract 1
- 239000011148 porous material Substances 0.000 abstract 1
- 239000012465 retentate Substances 0.000 abstract 1
- 238000011169 microbiological contamination Methods 0.000 description 24
- 238000000034 method Methods 0.000 description 23
- 241000894006 Bacteria Species 0.000 description 21
- 229940024606 amino acid Drugs 0.000 description 17
- 235000001014 amino acid Nutrition 0.000 description 17
- 239000012141 concentrate Substances 0.000 description 14
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 10
- 230000000694 effects Effects 0.000 description 7
- 229960002989 glutamic acid Drugs 0.000 description 6
- 238000000386 microscopy Methods 0.000 description 5
- 241000726221 Gemma Species 0.000 description 4
- 239000000919 ceramic Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000004907 flux Effects 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 230000003245 working effect Effects 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to the field of amino acid fermentation, and specifically discloses a preparation method of corn steep liquor. According to the preparation method of the invention, corn kernels are soaked in sulphurous acid, concentration is carried out to obtain a corn steep liquor, microfiltration is performed on the corn steep liquor by using a filter membrane having a pore size of 0.05-0.1mum until the concentration factor is 10-12 to obtain a filtrated liquid and a retentate, and the filtrated liquid is concentrated until a baume degree of 8-24 is achieved so as to obtain the corn steep liquor. According to the invention, the microfiltration is performed on the corn steep liquor before the preparation of the corn steep liquor, microorganisms which cause contamination with corn steep liquor fermentation are trapped, the prepared corn steep liquor can be used in the amino acid fermentation after undergoing high temperature sterilization, contamination degree of the corn steep liquor used in the amino acid fermentation is greatly reduced, contamination rate is reduced, and acid yield level of amino acid is increased at the same time.
Description
Technical field
The present invention relates to the amino acid fermentation field, a kind of preparation method of steeping water specifically is provided.
Background technology
Steeping water is meant that corn grain concentrates the tawny liquid that forms after sulfurous acid soaks, and wherein contains rich soluble albumen, growth hormone and some precursor substances, is the important compound material in the amino acid fermentation.Steeping water quality height not only influences the amino acid fermentation level, and with ferment whether microbiological contamination has much relations.
Existing preparation method obtains corn steep liquor with corn grain after sulfurous acid soaks, concentrate through multiple-effect then and obtain steeping water.The steeping water of existing method preparation generally just directly is applied in the amino acid production as the amino acid fermentation raw material behind the steam high-temperature sterilization.But; Often have some insolubles particles and mikrobe gemma in the steeping water of existing method preparation, in sterilization process, some bigger insoluble composition granule internal and external temperatures there are differences; The mikrobe that causes inside to comprise can not thoroughly be killed; Part kind gemma mikrobe can not be killed through conventional moist heat sterilization method fully in addition, grows the amino acid fermentation bacterial classification of (more than the 48h) for tolerating assorted bacterium poor-performing and fermentation period, and these residual mikrobes are often caused living contaminants in various degree; Cause the fermentation index to reduce even tank switching the also corresponding reduction of acid yield.Can not effectively reduce the living contaminants rate even improve sterilising temp or prolong sterilization time, can make the loss of nutritional ingredients of steeping water on the contrary because of long high temperature, production brings great influence to amino acid.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of preparation method of steeping water, make the steeping water of this preparing method's preparation can reduce the degree of amino acid fermentation microbiological contamination, and improve the amino acid acid yield.
For realizing above goal of the invention, the present invention provides following technical scheme:
A kind of preparation method of steeping water; Corn grain is soaked through sulfurous acid; Obtain corn steep liquor after concentrating, corn steep liquor uses the filter membrane micro-filtration of aperture as 0.05-0.1 μ m, when cycles of concentration is 10-12 times, stops; Obtain cleaner liquid and trapped fluid, it is promptly to get steeping water behind the 8-24 that cleaner liquid is concentrated into degree Beaume.
Corn steep liquor is soaked through sulfurous acid by corn grain according to this area ordinary method; Being concentrated into degree Beaume is that 3-5 promptly gets corn steep liquor; Wherein contain more insolubles particle and mikrobe gemma; The aperture of these assorted bacterium is generally between 0.5-5 μ m, and the present invention selects the filter membrane micro-filtration of 0.05-0.1 μ m for use, preferably selects the filter membrane micro-filtration of 0.07 μ m for use; Can hold back all bacteriums and gemma, solve effectively because of assorted bacterium and can not thoroughly have been killed the problem that causes amino acid microbiological contamination when utilizing the steeping water fermentation by high temperature.If the filter membrane aperture that the present invention adopts is less than 0.05 μ m; The micro-filtration effect does not have significant difference with the micro-filtration effect between the filter membrane of 0.05-0.1 μ m; But too small aperture makes the resistance of micro-filtration strengthen, and the time of consumption and cost increase, and are unfavorable for the production of steeping water; And if the filter membrane aperture has a small amount of mikrobe and sees through filter membrane greater than 0.1 μ m, influence the micro-filtration effect.
As preferably; Preparing method according to the invention also comprises the trapped fluid that directly obtains with the corn steep liquor micro-filtration is mixed with the water that volume is not more than said corn steep liquor volume 40%; Use the filter membrane micro-filtration of aperture as 0.05-0.1 μ m; The trapped fluid volume that to micro-filtration, obtains with directly obtain stopping when the trapped fluid volume is identical with the corn steep liquor micro-filtration, resulting cleaner liquid with directly obtain cleaner liquid and mix with the corn steep liquor micro-filtration, and then to be concentrated into degree Beaume be that 8-24 makes steeping water.Optimal technical scheme of the present invention is avoided single employing corn steep liquor micro-filtration to obtain cleaner liquid preparing steeping water, can farthest keep the nutritive ingredient in the steeping water, improves the quality of steeping water.As preferably, the volume of said water is 20% of a said corn steep liquor volume.
The cycles of concentration notion of micro-filtration according to the invention is known in this field, and promptly cycles of concentration=corn steep liquor volume/trapped fluid volume is preferably 11 times.Cycles of concentration is crossed low waste of making raw material easily, and the too high degree of difficulty that can increase micro-filtration.
The present invention with cleaner liquid in 55-76 ℃, preferred 65 ℃, vacuum tightness≤-0.1Mpa, carry out single-action or multiple-effect under preferred-0.095Mpa and concentrate and obtain steeping water, degree Beaume is 8-24, preferred degree Beaume is 20-22.
In addition, the temperature of micro-filtration according to the invention is controlled higher meeting and is caused in the corn steep liquor nutritive substance loss, and influences film core work-ing life.Temperature is controlled viscosity of sludge increase on the low side can cause difficulty in filtration to increase, and speed slows down, and cycles of concentration reduces, and the nutrition yield reduces in the filtering.So the temperature of micro-filtration according to the invention is preferably 40-60 ℃, more preferably 50-55 ℃.
Adopt the steeping water of preparing method's preparation according to the invention to be used for amino acid fermentation, fermented liquid does not almost have microbiological contamination, and uses the steeping water of traditional method preparation, and microbiological contamination situation in various degree appears in amino acid zymotic fluid, and the microbiological contamination rate is up to 60%.Simultaneously, adopt the steeping water of preparing method's preparation according to the invention to be used for amino acid fermentation, its amino acid acid yield is compared with the acid yield of the steeping water fermentation of adopting the traditional method preparation and is significantly improved.
Can know by above technical scheme; The present invention carried out micro-filtration to corn steep liquor before the preparation steeping water; Held back the mikrobe that causes microbiological contamination with steeping water fermentation, promptly can be used for amino acid fermentation behind the high-temperature sterilization, microbiological contamination degree when having reduced steeping water greatly and being used for amino acid fermentation; Reduce the microbiological contamination rate, improved the amino acid acid yield simultaneously.
Embodiment
The invention discloses a kind of preparation method of steeping water, those skilled in the art can use for reference this paper content, suitably improve processing parameter and realize.Special needs to be pointed out is that all similarly replace and change apparent to those skilled in the art, they all are regarded as and are included in the present invention.Method of the present invention is described through preferred embodiment, and the related personnel obviously can change or suitably change and combination methods and applications as herein described in not breaking away from content of the present invention, spirit and scope, realizes and use technology of the present invention.
Below in conjunction with embodiment, further set forth the present invention.
Embodiment 1: the preparation method of steeping water according to the invention and L-glutamic acid produce the acid test
1, preparation method
Preparing method of the present invention: corn grain is soaked through sulfurous acid, get corn steep liquor after concentrating, get 50m
3Baume is that 4.2 corn steep liquor is crossed ceramic membrane in the time of 55 ℃, and the filter membrane aperture is 0.07 μ m, and cycles of concentration is 10 times, and the acquisition trapped fluid is 5m
3, cleaner liquid is 45m
3, the micro-filtration flux is 125L/ (m
2H).With 45m
3Cleaner liquid carry out triple effect and concentrate, to be 73-75 ℃, vacuum tightness be-0.095Mpa to drop temperature, concentrates 5 times, obtains steeping water 9m
3, Baume is about 23.
The traditional preparation process method: behind the high-temperature sterilization with 50m
3Baume is that 4.2 corn steep liquor (source is with preparation method according to the invention) carries out triple effect and concentrates, and to be 73-75 ℃, vacuum tightness be-0.095Mpa to drop temperature, concentrates 5 times, obtains steeping water 10m
3, Baume is about 23.
2, L-glutamic acid produces the acid test
The steeping water of two kinds of method preparations in 1 is applied in the temperature sensitive glutamic acid fermentation process, and each 50 batches, inoculum size is identical, compares with the traditional technology steeping water, through seeding tank later stage bacterium liquid microscopy is observed.Jar batch bacterium liquid that uses the traditional technology steeping water is through microscopic examination, except that producing bacterium, it is thus clear that multiple a small amount of stock bacterium and middle stock bacterium.And the jar that uses technology steeping water of the present invention batch is got contemporaneously bacterium liquid and is carried out microscopic examination, is difficult to find and produces the visibly different mikrobe of bacterium form.
From fermentor tank microbiological contamination rate; Be used for each 50 batches of simultaneous test of fermentation through two kinds of steeping waters; Use in the battery of tests of traditional steeping water, 50 batches of test microbiological contamination rates are up to 60%, and wherein serious microbiological contamination (is produced below the sour 140g/L; The fermented liquid microscopy can be observed assorted bacterium) batch reach 5 batches, the 50 batches of tests are average, and to produce acid be 154g/L.And use the slight microbiological contamination of technology steeping water of the present invention (produce more than the sour 140g/L, the fermented liquid microscopy can be observed assorted bacterium) have only 2 batches, all the other no microbiological contaminations, the microbiological contamination rate can be ignored, the acid of 50 batches of average products of test is 171g/L.
Embodiment 2: the preparation method of steeping water according to the invention and Xie Ansuan produce the acid test
1, preparation method
Preparing method of the present invention: corn grain is soaked through sulfurous acid, get corn steep liquor after concentrating, get 240m
3Baume is that 4.2 corn steep liquor is crossed ceramic membrane in the time of 55 ℃, and the filter membrane aperture is 0.1 μ m, and cycles of concentration is 12 times, and the acquisition trapped fluid is 20m
3, cleaner liquid is 220m
3, the micro-filtration flux is 125L/ (m
2H).
With above-mentioned 20m
3Trapped fluid and 50m
3Water mixes, micro-filtration under these conditions, to the trapped fluid volume be 20m
3In time, stop, the 50m that this micro-filtration is obtained
3Cleaner liquid and above-mentioned 220m
3Cleaner liquid mixes and to carry out quadruple effect and concentrate, and to be 73-75 ℃, vacuum tightness be-0.095Mpa to drop temperature, concentrates 6 times, obtains steeping water 45m
3, Baume is about 22.
The traditional preparation process method: behind the high-temperature sterilization with 270m
3Baume is that 4.2 corn steep liquor (source is with preparation method according to the invention) carries out triple effect and concentrates, and to be 73-75 ℃, vacuum tightness be-0.095Mpa to drop temperature, concentrates 6 times, obtains steeping water 45m
3, Baume is about 23.
2, Xie Ansuan produces the acid test
The steeping water of two kinds of method preparations in 1 is used for the Xie Ansuan fermentation test.Respectively carry out 10 batches of test contrasts with traditional steeping water, under the identical situation of inoculum size, use batch seed tank culture cycle of technology steeping water of the present invention on average to shorten 2 hours, use batch shortening 10% of traditional steeping water.Pass through simultaneous test; Use batch fermentation of traditional technology steeping water to have 3 batches at 20-30 hour microbiological contamination tank switching; 3 batches of microbiological contaminations about 50-60 hour, microbiological contamination rate are up to 60%, and it is the microbiological contamination batch sour 60g/L of average product not; Use technology steeping water of the present invention on average to produce sour 65g/L, and do not occur microbiological contamination batch in the process of the test.
Embodiment 3: the preparation method of steeping water according to the invention and L-glutamic acid produce the acid test
1, preparation method
Preparing method of the present invention: corn grain is soaked through sulfurous acid, get corn steep liquor after concentrating, get 50m
3Baume is that 4.2 corn steep liquor is crossed ceramic membrane in the time of 55 ℃, and the filter membrane aperture is 0.05 μ m, and cycles of concentration is 10 times, and the acquisition trapped fluid is 5m
3, cleaner liquid is 45m
3, the micro-filtration flux is 125L/ (m
2H).
With above-mentioned 5m
3Trapped fluid and 20m
3Water mixes, micro-filtration under these conditions, to the trapped fluid volume be 5m
3In time, stop, the 20m that this micro-filtration is obtained
3Cleaner liquid and above-mentioned 45m
3Cleaner liquid mixes and to carry out list and concentrate, and to be 73-75 ℃, vacuum tightness be-0.095Mpa to drop temperature, concentrated 6.5 times, obtains steeping water 10m
3, Baume is about 22.
The traditional preparation process method: behind the high-temperature sterilization with 50m
3Baume is that 4.2 corn steep liquor (source is with preparation method according to the invention) carries out single-action and concentrates, and to be 73-75 ℃, vacuum tightness be-0.095Mpa to drop temperature, concentrates 5 times, obtains steeping water 10m
3, Baume is about 23.
2, L-glutamic acid produces the acid test
The steeping water of two kinds of method preparations in 1 is applied in the temperature sensitive glutamic acid fermentation process, and each 50 batches, inoculum size is identical, compares with the traditional technology steeping water, through seeding tank later stage bacterium liquid microscopy is observed.Jar batch bacterium liquid that uses the traditional technology steeping water is through microscopic examination, except that producing bacterium, it is thus clear that multiple a small amount of stock bacterium and middle stock bacterium.And the jar that uses technology steeping water of the present invention batch is got contemporaneously bacterium liquid and is carried out microscopic examination, is difficult to find and produces the visibly different mikrobe of bacterium form.
From fermentor tank microbiological contamination rate; Be used for each 50 batches of simultaneous test of fermentation through two kinds of steeping waters; Use in the battery of tests of traditional steeping water, 50 batches of test microbiological contamination rates are up to 60%, and wherein serious microbiological contamination (is produced below the sour 140g/L; The fermented liquid microscopy can be observed assorted bacterium) batch reach 7 batches, the 50 batches of tests are average, and to produce acid be 150g/L.And use technology steeping water of the present invention not have microbiological contamination, the acid of 50 batches of average products of test is 175g/L.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (8)
1. the preparation method of a steeping water; It is characterized in that, corn grain is soaked through sulfurous acid, obtain corn steep liquor after concentrating; Corn steep liquor uses the filter membrane micro-filtration of aperture as 0.05-0.1 μ m; When cycles of concentration is 10-12 times, stop, obtaining cleaner liquid and trapped fluid, it is promptly to get steeping water behind the 8-24 that cleaner liquid is concentrated into degree Beaume.
2. according to the said preparation method of claim 1, it is characterized in that, also comprise:
The said trapped fluid of claim 1 is mixed with the water that volume is not more than said corn steep liquor volume 40%; Use the filter membrane micro-filtration of aperture as 0.05-0.1 μ m; The trapped fluid volume that to micro-filtration, obtains stops when identical with the said trapped fluid volume of claim 1, resulting cleaner liquid behind the micro-filtration is obtained cleaner liquid with claim 1 mix.
3. according to the said preparation method of claim 2, it is characterized in that the volume of said water is 20% of a said corn steep liquor volume.
4. according to the said preparation method of claim 1, it is characterized in that the cycles of concentration of said micro-filtration is 11 times.
5. according to the said preparation method of claim 1, it is characterized in that said corn steep liquor degree Beaume is 3-5.
6. according to any said preparation method of claim 1-3, it is characterized in that said filter membrane aperture is 0.07 μ m.
7. according to any said preparation method of claim 1-3, it is characterized in that the temperature of said micro-filtration is 40-60 ℃.
8. according to the said preparation method of claim 7, it is characterized in that the temperature of said micro-filtration is 50-55 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104528304A CN102660595A (en) | 2011-12-29 | 2011-12-29 | Preparation method of corn steep liquor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104528304A CN102660595A (en) | 2011-12-29 | 2011-12-29 | Preparation method of corn steep liquor |
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| CN102660595A true CN102660595A (en) | 2012-09-12 |
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|---|---|---|---|
| CN2011104528304A Pending CN102660595A (en) | 2011-12-29 | 2011-12-29 | Preparation method of corn steep liquor |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103014085A (en) * | 2012-12-20 | 2013-04-03 | 通辽梅花生物科技有限公司 | Preparation method of corn syrup hydrolyzate |
| CN105494978A (en) * | 2015-12-21 | 2016-04-20 | 河南省新大牧业有限公司 | Method for preparing fermented pig feed through corn soaking solution and fermented pig feed prepared through method |
| CN107867759A (en) * | 2017-11-07 | 2018-04-03 | 周晓斌 | A kind of Novel sewage handles outer Ensure Liquid cultivation agent and preparation method thereof |
| CN110904163A (en) * | 2019-12-31 | 2020-03-24 | 内蒙古阜丰生物科技有限公司 | Method for improving lactic acid content of corn steep liquor |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101029083A (en) * | 2007-04-04 | 2007-09-05 | 菱花集团有限公司 | Technology for substituting disposable hydrate transferring raw material with corn slurry |
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2011
- 2011-12-29 CN CN2011104528304A patent/CN102660595A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101029083A (en) * | 2007-04-04 | 2007-09-05 | 菱花集团有限公司 | Technology for substituting disposable hydrate transferring raw material with corn slurry |
Non-Patent Citations (2)
| Title |
|---|
| NAVPREET SINGH等: "Fouling of a ceramic microfiltration membrane by corn starch hydrolysate", 《JOURNAL OF MEMBRANE SCIENCE》 * |
| 曲保雪: "玉米浸泡水中脂多糖及蛋白质的开发利用研究", 《中国优秀博硕士学位论文全文数据库（硕士）工程科技辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103014085A (en) * | 2012-12-20 | 2013-04-03 | 通辽梅花生物科技有限公司 | Preparation method of corn syrup hydrolyzate |
| CN105494978A (en) * | 2015-12-21 | 2016-04-20 | 河南省新大牧业有限公司 | Method for preparing fermented pig feed through corn soaking solution and fermented pig feed prepared through method |
| CN107867759A (en) * | 2017-11-07 | 2018-04-03 | 周晓斌 | A kind of Novel sewage handles outer Ensure Liquid cultivation agent and preparation method thereof |
| CN110904163A (en) * | 2019-12-31 | 2020-03-24 | 内蒙古阜丰生物科技有限公司 | Method for improving lactic acid content of corn steep liquor |
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Application publication date: 20120912 |