CN102783572A - Anti-stress feed for culturing Penaeus vannamei Boone in greenhouse - Google Patents

Anti-stress feed for culturing Penaeus vannamei Boone in greenhouse Download PDF

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Publication number
CN102783572A
CN102783572A CN2012102495635A CN201210249563A CN102783572A CN 102783572 A CN102783572 A CN 102783572A CN 2012102495635 A CN2012102495635 A CN 2012102495635A CN 201210249563 A CN201210249563 A CN 201210249563A CN 102783572 A CN102783572 A CN 102783572A
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Prior art keywords
vitamin
greenhouse
penaeus vannamei
powder
meal
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吴歌天
仇瑞胜
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NINGBO ZHENJIA BIOLOGICAL TECHNOLOGY Co Ltd
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NINGBO ZHENJIA BIOLOGICAL TECHNOLOGY Co Ltd
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Abstract

The invention relates to an anti-stress feed for culturing Penaeus vannamei Boone in a greenhouse. The anti-stress feed comprises 10-50% of fish meal, 5-10% of chicken powder, 3-10% of shrimp powder, 3-10% of a beer yeast, 10-30% of fermented soybean meal, 5-10% of squid powder, 10-16% of flour, 1-5% of fish oil, 1-3% of soybean phosphatide, 1-3% of betaine, 1-3% of a vitamin mixture, 2-4% of a mineral element mixture, 0.1-0.5% of Bacillus subtilis and 0.2-1% of taurine. According to the invention, the anti-stress feed selects feed raw materials having the advantages of high digestion and absorption utilization rate and strong food attraction against the growth and physiological digestion characteristics of the Penaeus vannamei Boone in the process of culturing in the greenhouse, trace nutritional elements comprising vitamins, mineral elements and the like needed by the healthy culture of the Penaeus vannamei Boone are reinforced, and simultaneously the Bacillus subtilis and the taurine are added, so shelling of the shrimps is promoted, the growth performances are improved, and the disease resistance and the stress resistance of the shrimps are enhanced. The mixed feed has the advantages of reliable sources of the raw materials, stable and safe quality, difficult water pollution, and suitableness for the large-scale culture of the Penaeus vannamei Boone in the greenhouse.

Description

Anti-stress feed for breeding penaeus vannamei boone in greenhouse
Technical Field
The invention relates to an anti-stress feed for penaeus vannamei bred in a greenhouse.
Technical Field
At present, the Penaeus vannamei (Penaeus vannamei) is cultivated in a greenhouseBoone) Has been gradually popularized in Shanghai culture areas of Jiangzhe province, the modelThe formula can effectively reduce large-scale disease outbreak induced by environmental stresses such as typhoon, rainfall and the like, and can carry out anti-season culture production to improve culture benefit.
The nutrient preparation is a key technology in the process of culturing the penaeus vannamei boone in the greenhouse, and directly influences the growth, development and survival rate of the penaeus vannamei boone. Therefore, whether the nutrition preparation is reasonable or not directly influences the culture benefit. At present, the feed for the Penaeus vannamei Boone in the greenhouse mainly uses the feed for the Penaeus vannamei Boone in an external pond, which often causes water quality to be easily polluted and increases the morbidity, and the raw material selection can not be scientifically selected according to the growth and physiological digestion characteristics of the Penaeus vannamei Boone in the greenhouse culture environment, so that the feed is not suitable for the ingestion of the Penaeus vannamei Boone, has low utilization rate, is easy to pollute water quality, and is not beneficial to the large.
The Chinese patent application (application No. 201110158200.6 application date: 2011-06-14) discloses a flaky prawn larva hatch feed, which comprises, by 100% of total mass, 10-50% of fish meal, 5-10% of chicken meal, 3-10% of egg meal, 3-10% of beer yeast, 10-30% of artemia, 5-10% of squid meal, 10-16% of flour, 1-3% of betaine, 1-5% of fish oil, 1-3% of soybean phospholipid, 1-3% of a vitamin mixture and 2-4% of a mineral element mixture; wherein the vitamin mixture consists of the following components, and each kilogram of the vitamin mixture contains: 30g of vitamin A, 32g, 60g of vitamin E, 34.5g of vitamin K, 130g of vitamin B, 22g of vitamin B, 660g of vitamin B, 124.5g of vitamin B, 20g of nicotinic acid, 20g of calcium pantothenate, 0.15g of biotin, 150g of inositol, 4g of folic acid, 275g of vitamin C and 337.85g of corncob meal; the mineral element mixture comprises 20g of copper sulfate, 100g of ferrous sulfate, 35g of zinc sulfate, 11g of manganese sulfate, 0.55g of cobalt chloride, 0.55g of sodium selenite, 20g of monopotassium phosphate, 20g of sodium dihydrogen phosphate and 792.9g of zeolite powder. The flaky prawn seedling starter feed disclosed by the patent adopts formula components to replace part of artemia bait so as to reduce the production cost of culturing prawn seedlings, but the patent still keeps 10-30% of the artemia bait, and although the cost can be accepted in the aspect of culturing seedlings, the cost is obviously considered further in the aspect of large-scale greenhouse culture. In addition, for greenhouse cultivation, due to the limitation of cultivation conditions, the cultivation water quality and the anti-stress performance of shrimps still need to be further considered.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects of the feed for the penaeus vannamei boone in the external pond, and provide the anti-stress feed for the penaeus vannamei boone cultured in the greenhouse, which is safe and healthy, has comprehensive nutrition and convenient use, can promote the shelling development of the penaeus vannamei boone under the environment of greenhouse culture, improves the anti-stress property and the growth performance, and regulates the cultured water quality.
In order to achieve the purpose, the invention adopts the following technical scheme:
the anti-stress feed for the penaeus vannamei cultivated in the greenhouse comprises the following components in percentage by weight:
10-50% of fish meal and 5-10% of chicken powder
3-10% of shrimp meal and 3-10% of beer yeast
Fermented soybean meal powder 10-30% and squid powder 5-10%
Flour 10-16% betaine 1-3%
Fish oil 1-5 wt% and soybean lecithin 1-3 wt%
Vitamin mixture 1-3% and mineral element mixture 2-4%
0.1-0.5% of bacillus subtilis and 0.2-1% of taurine;
wherein,
the vitamin mixture comprises the following components in gram/kg of vitamin mixture:
20-40% of vitamin A, 31.0-3.0% of vitamin D
50-70% of vitamin E and 34.0-5.0% of vitamin K
Vitamin B120-40 vitamin B21.0-3.0
650-70% of vitamin B, 124.0-5.0% of vitamin B
10-30 parts of nicotinic acid calcium pantothenate
Biotin 0.10-0.20 inositol 100-200
Folic acid 3.0-5.0 vitamin C200-300
300-500 parts of corncob meal;
the mineral element mixture comprises the following components in gram/kg of mineral element mixture:
10-30 parts of copper sulfate and 80-120 parts of ferrous sulfate
30-40 parts of zinc sulfate, 8-15 parts of manganese sulfate
Cobalt chloride 0.50-0.60 sodium selenite 0.50-0.60
10-30 parts of potassium dihydrogen phosphate and 10-30 parts of sodium dihydrogen phosphate
700-850 parts of zeolite powder.
Preferably, the anti-stress feed for the penaeus vannamei cultivated in the greenhouse comprises the following components in percentage by weight:
30% of fish meal and 10% of chicken powder
Shrimp meal 5% beer yeast 5%
16.3 percent of fermented soybean meal powder and 10 percent of sleeve-fish powder
Flour 10% and betaine 2%
Fish oil 3% and soybean lecithin 2%
Vitamin mixture 2% mineral element mixture 3%
0.2 percent of bacillus subtilis and 0.5 percent of taurine.
Most preferably, the vitamin mixture described above comprises the following components in grams per kilogram of vitamin mixture:
vitamin A30 vitamin D32
Vitamin E60 vitamin K34.5
Vitamin B130 vitamin B22
Vitamin B660 vitamin B124.5
Nicotinic acid 20 calcium pantothenate 20
Biotin 0.15 inositol 150
Folic acid 4 vitamin C275
Corncob meal 337.85.
Most preferably, the mineral element mixture described above comprises the following components in grams per kilogram of mineral element mixture:
copper sulfate 20 ferrous sulfate 100
Zinc sulfate 35 manganese sulfate 11
Cobalt chloride 0.55 sodium selenite 0.55
Potassium dihydrogen phosphate 20 sodium dihydrogen phosphate 20
Zeolite powder 792.9.
Aiming at the growth and physiological digestion characteristics of the penaeus vannamei boone in the greenhouse culture process, the anti-stress feed selects feed raw materials which are easy to digest, high in absorption and utilization rate and strong in feeding attraction, trace nutrient elements such as vitamins and mineral elements required by the healthy culture of the penaeus vannamei boone are enhanced, bacillus glumae and taurine are added, the shelling of the penaeus vannamei boone can be promoted, the growth performance is improved, the disease resistance and anti-stress capability of the penaeus vannamei boone are improved, the beneficial flora in intestinal tracts can be adjusted by adding bacillus subtilis, the feed utilization efficiency can be effectively improved, and the pollution of excrement to water quality is. The compound feed has reliable raw material source, stable and safe quality, is not easy to pollute water quality, and is suitable for large-scale greenhouse penaeus vannamei boone cultivation.
Detailed Description
Example 1
The vitamin mixture was produced according to the following formulation: (weight g/kg vitamin mixture)
Vitamin A30 vitamin D32
Vitamin E60 vitamin K34.5
Vitamin B130 vitamin B22
Vitamin B660 vitamin B124.5
Nicotinic acid 20 calcium pantothenate 20
Biotin 0.15 inositol 150
Folic acid 4 vitamin C275
Corncob meal 337.85
Weighing various vitamins according to the content in the formula, uniformly mixing the vitamins with the corncob meal, and sealing for later use.
The mineral element mixture was produced according to the following formulation: (weight g/kg mineral element mixture)
Copper sulfate 20 ferrous sulfate 100
Zinc sulfate 35 manganese sulfate 11
Cobalt chloride 0.55 sodium selenite 0.55
Potassium dihydrogen phosphate 20 sodium dihydrogen phosphate 20
Zeolite powder 792.9
Weighing various mineral salts according to the content in the formula, drying for 2 hours at 100 ℃, crushing until 95 percent of the mineral salts pass through a standard sieve of 100 meshes, mixing with zeolite powder uniformly, and sealing for later use.
The anti-stress feed for the greenhouse-cultured penaeus vannamei boone is produced according to the following formula (by weight percent):
30% of fish meal and 10% of chicken powder
Shrimp meal 5% beer yeast 5%
16.3 percent of fermented soybean meal powder and 10 percent of sleeve-fish powder
Flour 10% and betaine 2%
Fish oil 3% and soybean lecithin 2%
Vitamin mixture 2% mineral element mixture 3%
Bacillus subtilis 0.2% and taurine 0.5%
According to the formula, the feed is prepared and produced according to a patent (a processing technology of anti-stress feed for culturing penaeus vannamei boone in a greenhouse) applied by the inventor. The feed is sealed and subpackaged by 20kg of light-resistant composite woven bags and stored at normal temperature.
In a greenhouse culture environment, the anti-stress feed for the penaeus vannamei boone cultured in the greenhouse and the feed for the penaeus vannamei boone in a commodity external pond are used for feeding the penaeus vannamei boone larvae. Through 90-day cultivation experiments, the survival rate of an experimental group (fed with the anti-stress feed for the penaeus vannamei boone cultured in the greenhouse) is 52.6 percent, and the yield is 435 kg/mu; the survival rate of a control group (fed with commercial penaeus vannamei feed in an outer pond) is 45.3 percent, and the yield is 320 kg/mu. And the water quality of the culture water body of the experimental group is better than that of the control group.
The test result proves that: compared with the commercial outside pond feed for the penaeus vannamei boone, the anti-stress feed for the penaeus vannamei boone cultured in the greenhouse can increase the survival rate and the culture yield.
Example 2
The formulation of the vitamin mixture and mineral element mixture in this example is the same as in example 1, and the feed formulation is (in weight percent):
50% of fish meal and 5% of chicken powder
3% of shrimp meal and 3% of beer yeast
Fermented soybean meal powder 10% and sleeve-fish powder 5%
Flour 15.3% betaine 1%
Fish oil 1% and soybean lecithin 1%
Vitamin mixture 1% mineral element mixture 4%
Bacillus subtilis 0.4% and taurine 0.3%
The production method was the same as in example 1.
Through a 90-day cultivation test, the survival rate of the penaeus vannamei boone cultivated in the greenhouse produced by the formula is 48.6%, and the yield is 420 kg/mu.
Example 3
The feed formula comprises (by weight percent):
10% of fish meal and 6% of chicken powder
10% of shrimp meal and 10% of beer yeast
29.3 percent of fermented soybean meal powder and 6 percent of sleeve-fish powder
Flour 12% and betaine 3%
Fish oil 5% and soybean lecithin 3%
Vitamin mixture 3% mineral element mixture 2%
Bacillus subtilis 0.5% and taurine 0.2%
The rest is the same as in example 2.
Through a 90-day cultivation test, the survival rate of the penaeus vannamei boone cultivated in a greenhouse and produced by the formula is 55.2%, and the yield is 451 kg/mu.

Claims (4)

1. The anti-stress feed for the penaeus vannamei cultivated in the greenhouse is characterized by comprising the following components in percentage by weight:
10-50% of fish meal and 5-10% of chicken powder
3-10% of shrimp meal and 3-10% of beer yeast
Fermented soybean meal powder 10-30% and squid powder 5-10%
Flour 10-16% betaine 1-3%
Fish oil 1-5 wt% and soybean lecithin 1-3 wt%
Vitamin mixture 1-3% and mineral element mixture 2-4%
0.1-0.5% of bacillus subtilis and 0.2-1% of taurine;
wherein,
the vitamin mixture comprises the following components in gram/kg of vitamin mixture:
20-40% of vitamin A, 31.0-3.0% of vitamin D
50-70% of vitamin E and 34.0-5.0% of vitamin K
Vitamin B120-40 vitamin B21.0-3.0
650-70% of vitamin B, 124.0-5.0% of vitamin B
10-30 parts of nicotinic acid calcium pantothenate
Biotin 0.10-0.20 inositol 100-200
Folic acid 3.0-5.0 vitamin C200-300
300-500 parts of corncob meal;
the mineral element mixture comprises the following components in gram/kg of mineral element mixture:
10-30 parts of copper sulfate and 80-120 parts of ferrous sulfate
30-40 parts of zinc sulfate, 8-15 parts of manganese sulfate
Cobalt chloride 0.50-0.60 sodium selenite 0.50-0.60
10-30 parts of potassium dihydrogen phosphate and 10-30 parts of sodium dihydrogen phosphate
700-850 parts of zeolite powder.
2. The greenhouse-cultured Penaeus vannamei anti-stress feed as claimed in claim 1, is characterized by comprising the following components in percentage by weight:
30% of fish meal and 10% of chicken powder
Shrimp meal 5% beer yeast 5%
16.3 percent of fermented soybean meal powder and 10 percent of sleeve-fish powder
Flour 10% and betaine 2%
Fish oil 3% and soybean lecithin 2%
Vitamin mixture 2% mineral element mixture 3%
0.2 percent of bacillus subtilis and 0.5 percent of taurine.
3. The greenhouse-cultivated Penaeus vannamei anti-stress feed as claimed in claim 1 or 2, characterized in that the vitamin mixture comprises the following components in grams per kilogram of vitamin mixture:
vitamin A30 vitamin D32
Vitamin E60 vitamin K34.5
Vitamin B130 vitamin B22
Vitamin B660 vitamin B124.5
Nicotinic acid 20 calcium pantothenate 20
Biotin 0.15 inositol 150
Folic acid 4 vitamin C275
Corncob meal 337.85.
4. The greenhouse-cultivated Penaeus vannamei anti-stress feed as claimed in claim 1 or 2, characterized in that the mineral element mixture comprises the following components in grams per kilogram of mineral element mixture:
copper sulfate 20 ferrous sulfate 100
Zinc sulfate 35 manganese sulfate 11
Cobalt chloride 0.55 sodium selenite 0.55
Potassium dihydrogen phosphate 20 sodium dihydrogen phosphate 20
Zeolite powder 792.9.
CN2012102495635A 2012-07-19 2012-07-19 Anti-stress feed for culturing Penaeus vannamei Boone in greenhouse Pending CN102783572A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103005178A (en) * 2012-11-27 2013-04-03 洪仁作 Prawn nutrition intensifier
CN103385353A (en) * 2013-07-24 2013-11-13 中山大学 Penaeus vannamei compound feed special for high salinity cultivation and preparation method of compound feed
CN103583803A (en) * 2013-10-17 2014-02-19 蚌埠市丰牧牛羊肉制品有限责任公司 Feed for penaeus vannamei at shelling period and preparation method thereof
CN104757320A (en) * 2015-04-10 2015-07-08 烟台大乐饲料有限公司 Functional compound feed special for greenhouse cultivation of litopenaeus vannamei
CN105029101A (en) * 2015-08-29 2015-11-11 固镇县益民养殖专业合作社 Low-cost crucian feed
CN106798198A (en) * 2017-01-09 2017-06-06 宁波浙农农业科技有限公司 A kind of fermented feed base-material that can be used for seawater and brackish water prawn culturing
CN107637723A (en) * 2017-10-26 2018-01-30 贵州思南腾辉水产养殖有限公司 A kind of cray feed and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101124950A (en) * 2007-09-26 2008-02-20 中山大学 Low fish powder feed for litopenaeus vannamei
CN101978859A (en) * 2010-11-23 2011-02-23 上海海洋大学 Formula of macrobrachium nipponense feed and method for preparing macrobrachium nipponense compound feed from macrobrachium nipponense feed
CN102217733A (en) * 2011-06-14 2011-10-19 广东越群海洋生物研究开发有限公司 Flaky hatch feed for prawn seedlings

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101124950A (en) * 2007-09-26 2008-02-20 中山大学 Low fish powder feed for litopenaeus vannamei
CN101978859A (en) * 2010-11-23 2011-02-23 上海海洋大学 Formula of macrobrachium nipponense feed and method for preparing macrobrachium nipponense compound feed from macrobrachium nipponense feed
CN102217733A (en) * 2011-06-14 2011-10-19 广东越群海洋生物研究开发有限公司 Flaky hatch feed for prawn seedlings

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
《可持续水产养殖-资源、环境、质量-2003水产科技论坛论文集》 20040531 郭志勋等 《芽抱杆菌对凡纳对虾生长及免疫的影响》 第121-126页 1-4 , *
丁贤等: "《芽抱杆菌对凡纳对虾生长和消化酶活性的影响》", 《中国水产科学》 *
郭志勋等: "《芽抱杆菌对凡纳对虾生长及免疫的影响》", 《可持续水产养殖-资源、环境、质量-2003水产科技论坛论文集》 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103005178A (en) * 2012-11-27 2013-04-03 洪仁作 Prawn nutrition intensifier
CN103385353A (en) * 2013-07-24 2013-11-13 中山大学 Penaeus vannamei compound feed special for high salinity cultivation and preparation method of compound feed
CN103385353B (en) * 2013-07-24 2015-06-03 中山大学 Penaeus vannamei compound feed special for high salinity cultivation and preparation method of compound feed
CN103583803A (en) * 2013-10-17 2014-02-19 蚌埠市丰牧牛羊肉制品有限责任公司 Feed for penaeus vannamei at shelling period and preparation method thereof
CN104757320A (en) * 2015-04-10 2015-07-08 烟台大乐饲料有限公司 Functional compound feed special for greenhouse cultivation of litopenaeus vannamei
CN105029101A (en) * 2015-08-29 2015-11-11 固镇县益民养殖专业合作社 Low-cost crucian feed
CN106798198A (en) * 2017-01-09 2017-06-06 宁波浙农农业科技有限公司 A kind of fermented feed base-material that can be used for seawater and brackish water prawn culturing
CN107637723A (en) * 2017-10-26 2018-01-30 贵州思南腾辉水产养殖有限公司 A kind of cray feed and preparation method thereof

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Application publication date: 20121121