CN102781955A - Bt蛋白Cry4Cb2、其编码基因及应用 - Google Patents

Bt蛋白Cry4Cb2、其编码基因及应用 Download PDF

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CN102781955A
CN102781955A CN2010800166435A CN201080016643A CN102781955A CN 102781955 A CN102781955 A CN 102781955A CN 2010800166435 A CN2010800166435 A CN 2010800166435A CN 201080016643 A CN201080016643 A CN 201080016643A CN 102781955 A CN102781955 A CN 102781955A
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protein
gene
amino acid
insect
albumen
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CN102781955B (zh
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李平
郑爱萍
朱军
王玲霞
王世全
邓其明
李双成
刘怀年
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Sichuan Agricultural University
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Sichuan Agricultural University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Insects & Arthropods (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)

Abstract

本发明提供了一种新的Bt蛋白Cry4Cb2及其编码基因，所述蛋白是1)SEQ ID No.2所示的氨基酸序列组成的蛋白质，或2)SEQ ID No.2所示的氨基酸序列经取代、缺失和/或增加一个或几个氨基酸且具有同等活性的由1)所述蛋白质衍生的蛋白质。

Description

PCT国内申请，说明书已公开。

Claims (1)

  1. PCT国内申请，权利要求书已公开。
CN201080016643.5A 2009-04-13 2010-04-13 Bt蛋白Cry4Cb2、其编码基因及应用 Expired - Fee Related CN102781955B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201080016643.5A CN102781955B (zh) 2009-04-13 2010-04-13 Bt蛋白Cry4Cb2、其编码基因及应用

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CNA2009100816004A CN101591382A (zh) 2009-04-13 2009-04-13 Bt蛋白Cry4Cb2、其编码基因及应用
CN200910081600.4 2009-04-13
CN201080016643.5A CN102781955B (zh) 2009-04-13 2010-04-13 Bt蛋白Cry4Cb2、其编码基因及应用
PCT/CN2010/000484 WO2010118632A1 (zh) 2009-04-13 2010-04-13 Bt蛋白Cry4Cb2、其编码基因及应用

Publications (2)

Publication Number Publication Date
CN102781955A true CN102781955A (zh) 2012-11-14
CN102781955B CN102781955B (zh) 2014-01-01

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CNA2009100816004A Pending CN101591382A (zh) 2009-04-13 2009-04-13 Bt蛋白Cry4Cb2、其编码基因及应用
CN201080016643.5A Expired - Fee Related CN102781955B (zh) 2009-04-13 2010-04-13 Bt蛋白Cry4Cb2、其编码基因及应用

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CN (2) CN101591382A (zh)
WO (1) WO2010118632A1 (zh)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101591382A (zh) * 2009-04-13 2009-12-02 四川农业大学 Bt蛋白Cry4Cb2、其编码基因及应用
CN102584960B (zh) * 2011-12-31 2013-11-27 四川农业大学 一种Bt蛋白Cry70Aa1、其编码基因及应用
CN102584959B (zh) * 2011-12-31 2013-11-27 四川农业大学 一种Bt蛋白Cry70Ba1、其编码基因及应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004020636A1 (en) * 2002-08-29 2004-03-11 Monsanto Technology, Llc Nucleotide sequences encoding cry1bb proteins for enhanced expression in plants

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7449552B2 (en) * 2006-04-14 2008-11-11 Pioneer Hi-Bred International, Inc. Bacillus thuringiensis cry gene and protein
CN101591382A (zh) * 2009-04-13 2009-12-02 四川农业大学 Bt蛋白Cry4Cb2、其编码基因及应用

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004020636A1 (en) * 2002-08-29 2004-03-11 Monsanto Technology, Llc Nucleotide sequences encoding cry1bb proteins for enhanced expression in plants

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
周钢: "苏云金菌杀蚊新基因和菌株的筛选", 《职业与健康》, vol. 21, no. 2, 28 February 2005 (2005-02-28), pages 161 - 167 *
朱军等: "四川盆地生态区苏云金芽胞杆菌cry基因的鉴定及新型模式cry基因的克隆", 《微生物学报》, vol. 49, no. 3, 4 March 2009 (2009-03-04), pages 324 - 330 *

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Publication number Publication date
CN101591382A (zh) 2009-12-02
WO2010118632A1 (zh) 2010-10-21
CN102781955B (zh) 2014-01-01

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