CN102757916A - Efficient composite organic matter decomposing inoculant - Google Patents
Efficient composite organic matter decomposing inoculant Download PDFInfo
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- CN102757916A CN102757916A CN2012102360905A CN201210236090A CN102757916A CN 102757916 A CN102757916 A CN 102757916A CN 2012102360905 A CN2012102360905 A CN 2012102360905A CN 201210236090 A CN201210236090 A CN 201210236090A CN 102757916 A CN102757916 A CN 102757916A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses an efficient composite organic matter decomposing inoculant capable of accelerating decomposition of organic matters, belonging to the technical field of microbes. The efficient composite organic matter decomposing inoculant is prepared by mixing the following components in percentage by volume: 30% of Cellulomonas flavigena zymophyte solution, 30% of Bacillus stearothermophilus zymophyte solution and 40% of Bacillus subtilis zymophyte solution. The total viable count of the three strains is 1 billion/ml, and the microbial inoculant type is liquid. The strains are respectively cultured in a fermentation tank without foreign microbes, and then compounded to obtain the efficient composite organic matter decomposing inoculant. By matching the three strains, various organic matters can be quickly degraded and converted into available organic fertilizers containing multifunctional probiotics within 15 days or so, and thus, the invention overcomes the defect that the traditional degradation needs more than 3 months.
Description
Technical field
The present invention relates to the agent of becoming thoroughly decomposed of a kind of efficient compound organic matter material that quickens organic material composting, the microorganism belonging to genus technical field.Be particularly useful for becoming thoroughly decomposed to bio-organic fertilizer.
Background technology
Along with the intensification aggravation of aquaculture, large-scale plant is more and more, and the harmless treatment of feces of livestock and poultry or recycle become a big obstacle of restriction aquaculture development.At present, feces of livestock and poultry and all kinds of organic domestic refuse all are through hyperthermia drying or heap is rotten or directly return the mode of using in the field and handle naturally, piles corruption naturally and generally takes more than 3 months the time and can degrade.Can't become thoroughly decomposed and kill harmful pathogenic bacteria wherein completely but these processing modes are not only wasted the coal energy, cause the disease of farm crop to take place, or directly burn root and burn seedling.
Summary of the invention
In order to solve the pollution of serious day by day organic waste to environment, organic wastes such as degraded feces of livestock and poultry and agricultural crop straw in the shortest time reach the nuisanceless standard of producing organic fertilizer.The present invention provides the agent of becoming thoroughly decomposed of a kind of efficient compound organic matter material.Utilize the three plant height living microorganism bacterial classifications that filter out for many years; Through in the pure culture of fermentor tank middle-high density; After the ratio proportioning according to science; Adopt the mode of artificial inoculation that organic waste is rapidly heated to kill harmful microorganism wherein, all kinds of extracellular enzymes of the excretory larger molecular organics of can degrading, reach high efficiente callback utilization and can be by the purpose of crop absorption.
The fermenting speed of organic material composting agent and effect are mainly determined by bacterial classification classification, bacterial classification blend proportion and the number of viable of fermenting agent.Wherein amount of viable bacteria is mainly by the culture medium prescription of fermentation and the technical process decision of fermentative prodn.
The present invention realizes with following technical scheme: the agent of becoming thoroughly decomposed of a kind of efficient compound organic matter material, and it is to be formed by the zymocyte liquid proportional mixing that produces yellowish fiber Zymomonas mobilis, bacstearothermophilus, subtilis; The volume percent of each zymocyte liquid is: produce yellowish fiber Zymomonas mobilis 30%, bacstearothermophilus 30%, subtilis 40%;
The total viable count of above-mentioned three types of bacterial strains is 1,000,000,000/milliliter, and the microbial inoculum type is a liquid.
The become thoroughly decomposed preparation method of agent of above-mentioned efficient compound organic matter material: adopt and carry out composite method preparation after each strain bacterium cultivated respectively in the fermentor tank that does not have assorted bacterium, preparation flow is: test tube slant seed → eggplant bottle inclined-plane → first class seed pot → secondary seed jar → production fermentor tank → bacterium liquid composite;
Subtilis liquid cultural method is: in 100L seed fermentation jar, the method for sterilization is according to the medium sterilization of standard with two eggplant bottle bacterial classification inoculations of 250ml specification; Each controlled variable is a rotating speed: 200 rev/mins, ventilation is than being 0.5 (V/Vmin), and temperature is 35 ℃, and incubation time 8 hours can be seeded in the secondary seed jar and cultivate; Said first class seed pot substratum is: prime beef cream 0.5%, peptone 0.5%, NaCI 0.6%, pH 7.0-7.5; Said secondary seed training method is identical with the training method of first order seed recited above, and the volume of its fermentor tank is 1m
3Produce the cultivation of fermentor tank then: the fermentative prodn substratum is: steeping water 0.8%, bean cake powder 1%, food starch 1%, KH
2PO
40.2 %, manganous sulfate 0.02%, PH7.0-7.5; Sterilization; Top secondary seed is transferred in each fermention medium, and rotating speed is 200 rev/mins in 8 hours of the fermentation culture initial stage, and the later stage is changed 300 rev/mins into, 1 (V/Vmin) that after the 12nd hour, will ventilate than bring up to, and incubation time is 25 hours;
Bacstearothermophilus liquid cultural method is: in 100L seed fermentation jar, the method for sterilization is according to the medium sterilization of standard with two eggplant bottle bacterial classification inoculations of 250ml specification; Each controlled variable is a rotating speed: 200 rev/mins, ventilation is than being 0.5 V/Vmin, and temperature is 40 ℃, and incubation time 8 hours can be seeded in the secondary seed jar and cultivate; Said first class seed pot substratum is: prime beef cream 0.5%, peptone 0.5%, NaCI 0.6%, pH 7.0-7.5; Said secondary seed training method is identical with the training method of first order seed recited above, and fermention medium is: steeping water 0.8%, bean cake powder 1%, food starch 1%, KH
2PO
40.2 %, manganous sulfate 0.02%, PH7.0-7.5; The volume of its fermentor tank is 1m
3Produce the cultivation of fermentor tank then: according to the recipe configuration and the sterilization of fermention medium; Top secondary seed is transferred in each fermention medium; Rotating speed is 200 rev/mins in 8 hours of the fermentation culture initial stage; Later stage is changed 300 rev/mins into, after the 12nd hour, will ventilate than bringing up to 1 V/Vmin, and incubation time is 25 hours;
Producing yellowish fiber Zymomonas mobilis liquid cultural method is: in 100L seed fermentation jar, the method for sterilization is according to the medium sterilization of standard with two eggplant bottle bacterial classification inoculations of 250ml specification; Each controlled variable is a rotating speed: 200 rev/mins, ventilation is than being 0.5 V/Vmin, and temperature is 35 ℃, and incubation time 12 hours can be seeded in the secondary seed jar and cultivate; Said first class seed pot substratum is: NaCI 0.6%, ammonium sulfate 0.2%, potassium primary phosphate 0.05%, sal epsom 0.01%, potassium hydrogenphosphate 0.2%, calcium chloride 0.01%, Xylo-Mucine 2%, PH 7.0-7.5; Said secondary seed training method is identical with the training method of first order seed recited above, and its fermention medium is: Xylo-Mucine CMC-Na 2%; Na
2HPO
40.25%; KH
2PO
40.15 %; Peptone 0.25 %; PH 7.0-7.5; The volume of fermentor tank is 1m
3Produce the cultivation of fermentor tank then: according to the recipe configuration and the sterilization of fermentative prodn substratum; Top secondary seed is transferred in fermention medium; Rotating speed is 200 rev/mins in 8 hours of the fermentation culture initial stage; Later stage is changed 300 rev/mins into, 1 (V/Vmin) that after the 12nd hour, will ventilate than bring up to, and incubation time is 25 hours;
The composite of said bacterium liquid is meant that through behind microscope and the dull and stereotyped cultivation counting will press by three kinds of bacterium liquid that top method is produced and produce yellowish fiber Zymomonas mobilis 30%, bacstearothermophilus 30%, subtilis 40% mixing and packing, the bacterium numerical control is built in 1,000,000,000/ml.
The invention has the beneficial effects as follows: through the cooperation of above-mentioned three strain bacterium; All kinds of organic materialss can both rapid Degradation and Transformation be available organic fertilizer about 15 days; And contain multi-functional probiotic bacterium, broken the drawback of traditional zymotic needs time more than 3 months.
Embodiment
Three kinds of bacterial strains: product yellowish fiber Zymomonas mobilis (
Cellulomonas. flavigena), bacstearothermophilus (
Bacillus. stearothermophilus) and subtilis (
Bacillus. Subtilis).
Production technique and flow process: test tube slant seed → eggplant bottle inclined-plane → first class seed pot → secondary seed jar → production fermentor tank → bacterium liquid composite.
The preservation of inclined-plane seed and cultivation: top three strain bacterial classifications all pass through the test tube slant in 4 degrees centigrade of preservations, and when needs were produced, preservation in a day is cultivated in switching on the eggplant bottle inclined-plane of 200ml subsequent use.
First order seed is cultivated:
(1), subtilis and bacstearothermophilus: in 100L seed fermentation jar, the configuration of fermention medium is by following prescription with two eggplant bottles (the 250ml specification) bacterial classification inoculation, and the method for sterilization is according to the medium sterilization of standard.Each controlled variable is a rotating speed: 200 rev/mins, ventilation is than being 0.5 (V/Vmin), and temperature is 35 ℃ of subtilises, 40 ℃ of bacstearothermophiluses.Incubation time 8 hours can be seeded in the secondary seed jar and cultivate.
The first class seed pot substratum is: prime beef cream 0.5%, peptone 0.5%, NaCI 0.6%, pH 7.0-7.5;
Fermention medium is: steeping water 0.8%, bean cake powder 1%, food starch 1%, KH
2PO
4
0.2 %, manganous sulfate 0.02%, PH7.0-7.5;
(2), produce the yellowish fiber Zymomonas mobilis: vaccination ways, inoculum size, rotating speed, the same subtilis of culture temperature when of ventilating, incubation time is 12 hours.
First order seed substratum (shaking bottle and first class seed pot): NaCI 0.6%, ammonium sulfate 0.2%, potassium primary phosphate 0.05%, sal epsom 0.01%, potassium hydrogenphosphate 0.2%, calcium chloride 0.01%, Xylo-Mucine 2%, PH 7.0-7.5.
Fermention medium: Xylo-Mucine (CMC-Na) 2%; Na
2HPO
40.25%; KH
2PO
40.15 %; Peptone 0.25 %; PH 7.0-7.5.
Secondary seed is cultivated:
The training method of above one-level kind is the same, and just the volume of fermentor tank is 1m
3
Produce the cultivation of fermentor tank:
Recipe configuration and sterilization according to fermention medium; Ready each secondary seed is transferred in each fermention medium above inciting somebody to action; Rotating speed is 200 rev/mins in 8 hours of the fermentation culture initial stage, and the later stage is changed 300 rev/mins into, and 1 (V/Vmin) will ventilate than bring up to after the 12nd hour.The incubation time of each strain bacterium is 25 hours.
Bacterium liquid composite:
After cultivation is counted with flat board through microscope, will mix by three kinds of bacterium liquid that top step is produced and pack, the bacterium numerical control is built in 1,000,000,000/ml.
Test-results
Three strain bacterium in the product be respectively produce the yellowish fiber Zymomonas mobilis (
Cellulomonas. flavigena), bacstearothermophilus (
Bacillus. stearothermophilus) and subtilis (
Bacillus. Subtilis), behind organic materials, they have different effects respectively with the complex formulation artificial inoculation of this three strains bacterium.Primary stage of inoculation; Subtilis can start fermentation breeding rapidly fast; The temperature of heap body is elevated to about 35 degree; And proteolytic enzyme and the amylase secretion of subtilis are very competent, and albumen in the material of can degrading and polysaccharose substance change amino acid and oligosaccharides and monose into, are beneficial in the soil by plant absorbing.When temperature is elevated to 40 ℃, activated breeding and the metabolic activity of bacstearothermophilus, leavening temperature continue to be raise, high energy reaches 76 ℃, can kill disease bacterium and all kinds of worm's ovum in the material fully.Produce under hot conditions simultaneously that yellowish fiber Zymomonas mobilis excretory born of the same parents outer fiber is plain, hemicellulase and lignoenzyme can effectively decompose the macromolecular substance of difficult degradations such as xylogen and Mierocrystalline cellulose; And make such material be converted into soil ulmin rapidly, shorten the time of becoming thoroughly decomposed.Through the cooperation of above-mentioned three strain bacterium, all kinds of organic materialss can both rapid Degradation and Transformation be available organic fertilizer about 15 days, and contain multi-functional probiotic bacterium, have broken the drawback of traditional zymotic needs time more than 3 months.
Claims (2)
1. efficient compound organic matter material agent of becoming thoroughly decomposed is characterized in that, it is to be formed by the zymocyte liquid proportional mixing that produces yellowish fiber Zymomonas mobilis, bacstearothermophilus, subtilis; The volume percent of each zymocyte liquid is: produce yellowish fiber Zymomonas mobilis 30%, bacstearothermophilus 30%, subtilis 40%;
The total viable count of above-mentioned three types of bacterial strains is 1,000,000,000/milliliter, and the microbial inoculum type is a liquid; Employing is carried out composite method preparation after each strain bacterium is cultivated respectively in the fermentor tank that does not have assorted bacterium.
2. the described efficient compound organic matter material of claim 1 preparation method of agent of becoming thoroughly decomposed; It is characterized in that; Employing is carried out composite method preparation after each strain bacterium is cultivated respectively in the fermentor tank that does not have assorted bacterium, preparation flow is: test tube slant seed → eggplant bottle inclined-plane → first class seed pot → secondary seed jar → production fermentor tank → bacterium liquid composite;
Subtilis liquid cultural method is: in 100L seed fermentation jar, the method for sterilization is according to the medium sterilization of standard with two eggplant bottle bacterial classification inoculations of 250ml specification; Each controlled variable is a rotating speed: 200 rev/mins, ventilation is than being 0.5 (V/Vmin), and temperature is 35 ℃, and incubation time 8 hours can be seeded in the secondary seed jar and cultivate; Said first class seed pot substratum is: prime beef cream 0.5%, peptone 0.5%, NaCI 0.6%, pH 7.0-7.5; Said secondary seed training method is identical with the training method of first order seed recited above, and the volume of its fermentor tank is 1m
3Produce the cultivation of fermentor tank then: the fermentative prodn substratum is: steeping water 0.8%, bean cake powder 1%, food starch 1%, KH
2PO
40.2 %, manganous sulfate 0.02%, PH7.0-7.5; Sterilization; Top secondary seed is transferred in each fermention medium, and rotating speed is 200 rev/mins in 8 hours of the fermentation culture initial stage, and the later stage is changed 300 rev/mins into, 1 (V/Vmin) that after the 12nd hour, will ventilate than bring up to, and incubation time is 25 hours;
Bacstearothermophilus liquid cultural method is: in 100L seed fermentation jar, the method for sterilization is according to the medium sterilization of standard with two eggplant bottle bacterial classification inoculations of 250ml specification; Each controlled variable is a rotating speed: 200 rev/mins, ventilation is than being 0.5 V/Vmin, and temperature is 40 ℃, and incubation time 8 hours can be seeded in the secondary seed jar and cultivate; Said first class seed pot substratum is: prime beef cream 0.5%, peptone 0.5%, NaCI 0.6%, pH 7.0-7.5; Said secondary seed training method is identical with the training method of first order seed recited above, and fermention medium is: steeping water 0.8%, bean cake powder 1%, food starch 1%, KH
2PO
40.2 %, manganous sulfate 0.02%, PH7.0-7.5; The volume of its fermentor tank is 1m
3Produce the cultivation of fermentor tank then: according to the recipe configuration and the sterilization of fermention medium; Top secondary seed is transferred in each fermention medium; Rotating speed is 200 rev/mins in 8 hours of the fermentation culture initial stage; Later stage is changed 300 rev/mins into, after the 12nd hour, will ventilate than bringing up to 1 V/Vmin, and incubation time is 25 hours;
Producing yellowish fiber Zymomonas mobilis liquid cultural method is: in 100L seed fermentation jar, the method for sterilization is according to the medium sterilization of standard with two eggplant bottle bacterial classification inoculations of 250ml specification; Each controlled variable is a rotating speed: 200 rev/mins, ventilation is than being 0.5 V/Vmin, and temperature is 35 ℃, and incubation time 12 hours can be seeded in the secondary seed jar and cultivate; Said first class seed pot substratum is: NaCI 0.6%, ammonium sulfate 0.2%, potassium primary phosphate 0.05%, sal epsom 0.01%, potassium hydrogenphosphate 0.2%, calcium chloride 0.01%, Xylo-Mucine 2%, PH 7.0-7.5; Said secondary seed training method is identical with the training method of first order seed recited above, and its fermention medium is: Xylo-Mucine CMC-Na 2%; Na
2HPO
40.25%; KH
2PO
40.15 %; Peptone 0.25 %; PH 7.0-7.5; The volume of fermentor tank is 1m
3Produce the cultivation of fermentor tank then: according to the recipe configuration and the sterilization of fermentative prodn substratum; Top secondary seed is transferred in fermention medium; Rotating speed is 200 rev/mins in 8 hours of the fermentation culture initial stage; Later stage is changed 300 rev/mins into, 1 (V/Vmin) that after the 12nd hour, will ventilate than bring up to, and incubation time is 25 hours;
The composite of said bacterium liquid is meant through behind microscope and the dull and stereotyped cultivation counting; To press the mixed and the packing of producing yellowish fiber Zymomonas mobilis 30%, bacstearothermophilus 30%, subtilis 40% by three kinds of bacterium liquid that top method is produced, the bacterium numerical control is built in 1,000,000,000/ml.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103172420A (en) * | 2012-11-08 | 2013-06-26 | 曾凡强 | Broad-spectrum applicable organic material decomposition agent |
| CN113773986A (en) * | 2021-08-26 | 2021-12-10 | 郑州轻工业大学 | Microbial inoculum for quickly raising temperature of organic solid waste aerobic fermentation in winter and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1800357A (en) * | 2005-12-12 | 2006-07-12 | 青岛地恩地生物科技有限公司 | Composite bacterium agent for organic material fermentation |
| CN101054552A (en) * | 2007-04-05 | 2007-10-17 | 清华大学 | Microorganism composite bacterium agent and preparation method and application thereof |
| CN101294141A (en) * | 2007-04-28 | 2008-10-29 | 上海四季生物科技有限公司 | A set of living body microorganism preparations for preparing composite microorganism fertilizer and preparation method thereof |
-
2012
- 2012-07-10 CN CN2012102360905A patent/CN102757916A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1800357A (en) * | 2005-12-12 | 2006-07-12 | 青岛地恩地生物科技有限公司 | Composite bacterium agent for organic material fermentation |
| CN101054552A (en) * | 2007-04-05 | 2007-10-17 | 清华大学 | Microorganism composite bacterium agent and preparation method and application thereof |
| CN101294141A (en) * | 2007-04-28 | 2008-10-29 | 上海四季生物科技有限公司 | A set of living body microorganism preparations for preparing composite microorganism fertilizer and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103172420A (en) * | 2012-11-08 | 2013-06-26 | 曾凡强 | Broad-spectrum applicable organic material decomposition agent |
| CN113773986A (en) * | 2021-08-26 | 2021-12-10 | 郑州轻工业大学 | Microbial inoculum for quickly raising temperature of organic solid waste aerobic fermentation in winter and preparation method thereof |
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