CN1027083C - Technology for preparing nutritive liquor with solid culture of Golden mushroom - Google Patents
Technology for preparing nutritive liquor with solid culture of Golden mushroom Download PDFInfo
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- CN1027083C CN1027083C CN89101223A CN89101223A CN1027083C CN 1027083 C CN1027083 C CN 1027083C CN 89101223 A CN89101223 A CN 89101223A CN 89101223 A CN89101223 A CN 89101223A CN 1027083 C CN1027083 C CN 1027083C
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Abstract
The present invention relates to development and utilization of edible fungus, particularly to a technique for making nutrient solution from solid culture of flammulina velutipes, which is characterized in that the technique comprises: mother strains of flammulina velutipes are transplanted; original strains are cultured in an enlarging mode; culture strains are cultured (culture media of both the original strains and the culture strains use wheat as the principal raw material); after mycelia completely grow, large quantities of primordia start to be discharged and leached; the enzymolysis and the saccharifying are carried out; the filtering and the concentrating are carried out. The production technique of the present invention has the advantages of short production periodicity, low cost, high extraction yield and rich nutrition of the finished product. The nutrient solution made by the production technique contains 18 free amino acids, a plain mushroom element, various vitamins, various trace elements, etc. and has wide prospects of development and application in the aspects of nutriments, health foods, medicines, biochemical reagents, etc.
Description
The present invention relates to the edible mushrooms development and use, particularly make the technology of nutritive medium with the needle mushroom solid culture.
In the prior art, people develop the processing that only is confined to sporophore to the utilization of needle mushroom, as the " Jinzhengu " edible fungus beverage making method of Japanese Patent JP7477092 description.
Task of the present invention is the traditional method that will change the needle mushroom food-processing, designs a kind of technology of making nutritive medium with the needle mushroom solid culture.This production technique cycle is short, cost is low, extracts the yield height, and finished product are nutritious.
The technological process of production of the present invention is: needle mushroom is female plants transplantings → original seed enlarged culturing → cultivar and cultivates long saturating, the original hase of (substratum of original seed and cultivar all is main raw material with the wheat) → treat mycelia and begin discharging lixiviate → enzymatic saccharifications → filtering and concentrating after a large amount of the appearance.
The concrete steps of production technique of the present invention:
1, the good needle mushroom bacterial strain of screening.
2, the female kind transplanted.
3, original seed enlarged culturing.
4, cultivar is cultivated.
The substratum of original seed and cultivar all is main raw material with the wheat.A kind of prescription of this process choice is:
100 parts of wheats;
Yeast extract paste 0.04-0.10 part;
0.5 part of glucose;
Lime carbonate 0.5-0.8 part;
Dipotassium hydrogen phosphate 0.01-0.05 part;
Secondary ammonium phosphate 0.01-0.03 part;
Sodium-chlor 0.1-0.3 part;
Sal epsom 0.01-0.03 part;
Sodium Selenite 0.05-2ppm
Zinc sulfate 0.5-10ppm
PH6.0-6.4(regulates with hydrochloric acid or sodium hydroxide)
5, discharging lixiviate.
Treat that mycelia is long saturating, after original hase begins a large amount of the appearance, draw out needle mushroom culture (comprising culture material, mycelium and sporophore etc.) → with the needle mushroom culture and discerp and insert hydrolyzer → adding and be equivalent to the abundant mixing of purifying waste water of 1～3 times of this material → regulate potential of hydrogen with lactic acid or citric acid and reach pH4～5 temperature is controlled at 39～69 ℃, 3～6 hours → drop of insulation goes out vat liquor for the first time; Add and be equivalent to purifying waste water of 1 times of culture, repeat above-mentioned steps, drop goes out vat liquor for the second time; The solution that merges twice lixiviate at last.
6, enzymatic saccharification.
The solution of twice lixiviate is poured in the stainless steel jacketed kettle → is added into the αDian Fenmei that is equivalent to extracting solution 0.01～0.05%, and be warming up to rapidly 70～90 ℃ → keep after 15～40 minutes and boil → be cooled to rapidly 40～60 ℃ and add the saccharifying enzyme be equivalent to solution 0.04～0.06% again, act on add the constant basket of iodine liquid to solution till → make solution be warming up to again 85～90 ℃ → kept 10～20 minutes.
7, filter, concentrate.
With plate-and-frame filter press with above-mentioned solution filter → filter back gained solution with the centrifugal thin-film thickener be evaporated to 20～40 ℃ Brix24 ° → again concentrated solution is boiled → is packaged in while hot in the container of sterilizing.
Embodiments of the invention:
1) wheat is drawn behind the wash clean with water purification soaked 36 hours, pick up and drain.
2) by following prescription:
10 kilograms of wheats;
Yeast extract paste 5 grams
Glucose 50 grams;
Lime carbonate 60 grams
Dipotassium hydrogen phosphate 3 grams;
Secondary ammonium phosphate 1 gram;
Sodium-chlor 10 grams;
Sal epsom 1 gram;
1 milliliter of 1% Sodium Selenite liquid;
4 milliliters of 1% zinc sulfate solutions;
Weighing is item by item fully regulated potential of hydrogen with hydrochloric acid or sodium hydroxide behind the mixing and is reached pH6.2～6.4.
3) above-mentioned material is sub-packed in the mushroom bottle, every bottle 220 restrains → has filled in tampon, wraps waterproof paper, places pressure kettle, with 121 ℃ of temperature, pressure 10.1 * 10
4Needle mushroom original seed → place 22～28 ℃ of greenhouses to cultivate 20 days is inserted in Pa sterilization 30～45 minutes → take out cooling back, and initial stage room temperature control is high slightly, after the mycelia vigorous growth, room temperature is low slightly → treat interior the mycelia length of bottle thoroughly, original hase begins to get final product when occurring in a large number discharging.
4) culture in the bottle is dug out put into Stainless Steel Kettle, add 25 kilograms and purify waste water, and smash the culture mixing → potential of hydrogen of feed liquid is transferred to pH4.2 → heat to 40 ℃ to pieces, be incubated 5 hours → leach vat liquor for the first time with lactic acid.
5) add and be equivalent to purifying waste water of one times of filter residue volume, the mixing → potential of hydrogen of feed liquid is transferred to pH4.2 → heat to 40 ℃ with lactic acid is incubated 4 hours → leach the vat liquor second time.
6) merge the vat liquor that leaches for twice, add the αDian Fenmei that is equivalent to vat liquor 0.01% → heat to 85 ℃, keep 20 minutes → heat again to seethe with excitement → being cooled to 41 ℃ rapidly, adding is equivalent to the saccharifying enzyme of solution 0.04% again; Act on add the constant basket of iodine liquid to solution till, make solution be warming up to 85 ℃ again, kept 10 minutes.
7) with small-sized flame filter press filter → be concentrated into 20 ° of Brix with the centrifugal thin-film thickener again, must concentrated solution 6.5 * 10
-3→ boil in the concentrated solution → container of sterilizing of packing into while hot and preserve.
Through the check of centralab of Fujian Academy of Agricultural Sciences, above-mentioned gained concentrated solution contains following 18 kinds of total free aminoacids (kg/m
3):
Aspartic acid 0.298;
Threonine 0.2636;
Serine 0.2864;
L-glutamic acid 2.7174;
Proline(Pro) 0.766;
Glycine 0.4753;
L-Ala 0.702;
Gelucystine 0.2443;
Knot page or leaf propylhomoserin 0.6262;
Methionine(Met) 0.5026;
Isoleucine 0.482;
Leucine 0.525;
Tryptophane 0.782;
Phenylalanine 0.687;
Methionin 0.527;
Tyrosine 0.203;
Histidine 0.1335;
Arginine 0.7334.
In addition, also contain provitamin A, B
1, B
2With C and polysaccharide material.
Production technique of the present invention has changed by the needle mushroom sporophore extracts nutraceutical traditional method, directly fully extracts natural nutrient component from the needle mushroom culture, and therefore with short production cycle, cost is low, extracts the yield height, and finished product are nutritious.
The nutritive medium that this production technique is produced contains 18 kinds of total free aminoacidss (wherein 8 kinds is that human body is necessary), Flammulin, multivitamin and trace element etc.Remove and to supply food service industry preparing natural nourishing drink, strengthen outside the nutritive food such as cake, candy, bread, also can develop and utilize at aspects such as protective foods, medicine, biochemical reagents.
Prove that according to clinical example the natural nutrient fluid that production technique of the present invention is produced is to cancer, tumour, diabetes, early stage uremia and follow assorted disease, chronic gastritis, atrophic gastritis and neurasthenia etc. all has obvious curative effects.
The technological process of production of the present invention is not only applicable to the development and use of needle mushroom, and is applicable to edible mushroomss such as mushroom, Pleurotus sajor-caju, flat mushroom, Hericium erinaceus (Bull. Ex Fr.) Pers..
Claims (1)
1, a kind ofly make the technology of nutritive medium, comprise that female kind of needle mushroom plant-original seed enlarged culturing and cultivar cultivation more, it is characterized in that with the needle mushroom solid culture,
The prescription of the substratum of cultivar is:
100 parts of wheats;
Yeast extract paste 0.04-0.10 part;
0.5 part of glucose;
Lime carbonate 0.5-0.8 part;
Dipotassium hydrogen phosphate 0.01-0.05 part;
Secondary ammonium phosphate 0.01-0.03 part;
Sodium-chlor 0.1-0.3 part;
Sal epsom 0.01-0.03 part;
Sodium Selenite 0.05-2ppm
Zinc sulfate 0.5-10ppm
PH6.0-6.4 (regulating) with hydrochloric acid or sodium hydroxide
Treat that mycelia is long saturating, after original hase begins a large amount of the appearance, carry out
Discharging lixiviate: draw out needle mushroom solid culture (comprising culture material, mycelium and sporophore etc.) → with the needle mushroom solid culture, discerping and insert hydrolyzer → adding is equivalent to the abundant mixing of purifying waste water of 1～3 times of this material → regulate potential of hydrogen with lactic acid or citric acid and reaches pH4～5-temperature is controlled at 39～69 ℃, 3～6 hours → drop of insulation goes out for the first time vat liquor → add again and is equivalent to purifying waste water of 1 times of culture, repeat above-mentioned steps, drop goes out for the second time vat liquor-merge the at last solution of twice lixiviate;
Enzymatic saccharification: pour in the stainless steel jacketed kettle solution of twice lixiviate into one and add the αDian Fenmei that is equivalent to extracting solution 0.01～0.05%, and be warming up to rapidly 70～90 ℃ → keep after 15～40 minutes and boil → be cooled to rapidly 40～60 ℃, → add the saccharifying enzyme be equivalent to solution 0.04～0.06% again, act on add the constant basket of iodine liquid to solution till-make solution be warming up to again 85～90 ℃ → kept 10～20 minutes;
Filter, concentrate; With plate-and-frame filter press above-mentioned solution being filtered-filter back gained solution is evaporated to 20-24 ° of Brix-with the centrifugal thin-film thickener and concentrated solution is boiled again-be packaged in while hot in the container of sterilizing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN89101223A CN1027083C (en) | 1989-03-03 | 1989-03-03 | Technology for preparing nutritive liquor with solid culture of Golden mushroom |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN89101223A CN1027083C (en) | 1989-03-03 | 1989-03-03 | Technology for preparing nutritive liquor with solid culture of Golden mushroom |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1043610A CN1043610A (en) | 1990-07-11 |
| CN1027083C true CN1027083C (en) | 1994-12-21 |
Family
ID=4854193
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN89101223A Expired - Fee Related CN1027083C (en) | 1989-03-03 | 1989-03-03 | Technology for preparing nutritive liquor with solid culture of Golden mushroom |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1027083C (en) |
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| CN101648836B (en) * | 2009-08-31 | 2012-12-05 | 河北科技大学 | Water replenishing nutrient of edible mushroom |
| CN101822172A (en) * | 2010-05-25 | 2010-09-08 | 上海市农业科学院 | Needle mushroom fruiting and breeding method |
| CN102498935B (en) * | 2011-10-14 | 2013-10-23 | 福建农林大学 | Method for preparing flammulina velutipes nutrient solution by utilizing remains of food processing |
| CN102715408A (en) * | 2012-07-03 | 2012-10-10 | 西安文理学院 | Preparation method of needle mushroom amazake |
| CN102805335B (en) * | 2012-07-20 | 2015-07-29 | 北京厚文知识产权顾问有限公司 | A kind of preparation method of edible fungus health product |
| CN103214294B (en) * | 2013-03-08 | 2014-08-27 | 湖北富士峰生物科技有限公司 | Secondary fruiting nutrient solution for golden mushroom and preparation method |
| CN103570422B (en) * | 2013-11-08 | 2016-02-03 | 菏泽学院 | A kind of edible fungus granules bacterial classification formula and processing technology |
| CN104058801B (en) * | 2014-06-17 | 2016-08-24 | 高小文 | A kind of preparation method of ammino acid liquid fertilizer |
| CN104206173B (en) * | 2014-08-29 | 2016-09-07 | 上海市农业科学院 | A kind of method utilizing wild Hericium erinaceus cultivating in bag |
| CN105557220A (en) * | 2014-10-08 | 2016-05-11 | 彭宝礼 | Scalar selenium-enriched Grifola frondosa factory production technique |
| CN105557292A (en) * | 2014-10-08 | 2016-05-11 | 彭宝礼 | Scalar selenium-enriched Lentinus edodes factory production technique |
| CN105474985A (en) * | 2014-10-08 | 2016-04-13 | 彭宝礼 | Industrialized production technique for scalar selenium-rich pleurotus eryngii |
| CN104585734B (en) * | 2014-12-30 | 2018-04-03 | 上海大山合菌物科技股份有限公司 | A kind of asparagus slurries zymolyte and preparation method thereof |
-
1989
- 1989-03-03 CN CN89101223A patent/CN1027083C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1043610A (en) | 1990-07-11 |
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