CN101440383A - Fermentation process for producing gamma-linolenic acid with corn starch syrup as principal raw material - Google Patents

Fermentation process for producing gamma-linolenic acid with corn starch syrup as principal raw material Download PDF

Info

Publication number
CN101440383A
CN101440383A CNA2007101583943A CN200710158394A CN101440383A CN 101440383 A CN101440383 A CN 101440383A CN A2007101583943 A CNA2007101583943 A CN A2007101583943A CN 200710158394 A CN200710158394 A CN 200710158394A CN 101440383 A CN101440383 A CN 101440383A
Authority
CN
China
Prior art keywords
culture
fermentation
corn starch
starch syrup
linolenic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA2007101583943A
Other languages
Chinese (zh)
Inventor
靳素英
杨建成
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CNA2007101583943A priority Critical patent/CN101440383A/en
Publication of CN101440383A publication Critical patent/CN101440383A/en
Pending legal-status Critical Current

Links

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to a biotechnology process for producing gamma-linolenic acid, in particular to a fermentation process for producing gamma-linolenic acid by taking corn starch syrup as a main raw material. The aim of the invention is to disclose a fermentation process for producing the gamma-linolenic acid by taking corn processing products which is corn starch syrup as a main raw material so as to lower production cost of the gamma-linolenic acid, improve yield, and produce side products which can be used as highly efficient organic fertilizer, animal feed and the like at the same time. The technical proposal adopted in the invention comprises: 1, obtaining of high yield strain: cunninghamella echinulata separated spore is subjected to microwave processing and ultraviolet mutagenesis to obtain high-yield strain; and 2, production fermentation process flow of the gamma-linolenic acid: (1) expanding propagation of strain; (2) preparation of seed liquid; (3) fermentation culture; and (4) collection and processing of thalli.

Description

With the corn starch syrup is the zymotechnique of main material production gamma-linolenic acid
Technical field:
The present invention relates to utilize biotechnology to produce the technology of gamma-linolenic acid, saying so more specifically with the corn starch syrup is the zymotechnique of main material production gamma-linolenic acid.
Background technology:
Gamma-linolenic acid (γ-Linolenic acid, GLA), chemistry is called 6,9, the 12-punicic acid, molecular formula is C 18H 30O 2, be a kind of indispensable fatty acid of human body.Gamma-linolenic acid is a structured material of forming human tissue cell's film, also is the precursor substance of biologically active substances such as synthesis of prostaglandins and leukotriene.Studies show that in recent years, gamma-linolenic acid has physiological function and pharmacological action widely, as: promote that infant's brain and eyesight grow, strengthen that insulin action, antibiotic, anti-HIV infect, antitumor, anti-inflammatory, anti-oxidant, antithrombotic, anti-atherosis, reducing blood-fat, treatment hypertension, anti-ageing, whiten or the like.Gamma-linolenic acid now has been widely used in fields such as medicine, food, makeup.Gamma-linolenic acid is present in the human body great majority tissue, but lifetime is very short, and content seldom.Needed by human obtains enough gamma-linolenic acids from food, otherwise will influence normal physiological function, causes multiple disease.
At present, two kinds of methods are adopted in the production of gamma-linolenic acid both at home and abroad usually: the one, from the plant of being rich in gamma-linolenic acid, extract (routine root of Redsepal Eveningprimrose, Borrago officinalis etc.), but be subjected to the influence of factors such as region, weather bigger because of plant biomass, this production method has certain limitation.Another kind is to adopt Production by Microorganism Fermentation, because this production method is not limited by the raw material and the place of production, and with short production cycle, the output height, cost is low, is the focus of producing GLA research now in the world.
Current, the gamma-linolenic acid of the microbial fermentation of report production both at home and abroad culture medium mainly is sucrose or glucose, and production efficiency is lower, is generally 30%, causes final cost higher.
Summary of the invention:
The objective of the invention is to disclose that a kind of corn processed product that utilizes---corn starch syrup is the zymotechnique of main material production gamma-linolenic acid, to reduce the gamma-linolenic acid production cost, improve productive rate, produce the byproduct that can be used as efficient organic fertilizer, animal-feed etc. simultaneously.
For achieving the above object, the present invention adopts following technical scheme:
1. high-yield strains obtains
With cunninghamella echinulata (formal name used at school Cunninghamella echinulata, the microbial strains preservation center preservation of country of institute of microbiology of the Chinese Academy of Sciences, preserving number: AS3.2473) separate spore after microwave treatment,, obtain superior strain again through ultraviolet mutagenesis; This bacterial strain initial stage on the potato substratum is white cotton wool shape, after gradually become lark, the flourishing racemosus of mycelium; Compare with original strain, fast growth, the spore amount is many, and fermentation back bacterium powder yield can reach 30～39g/L;
2. gamma-linolenic acid is produced the zymotechnique flow process
1. bacterial classification expands numerous: making spore content is 1-2 * 10 5Individual spore suspension;
2. seed liquor prepares: the suspension for preparing is seeded in the seeding tank cultivates; Seed culture medium consists of: corn starch syrup 2-5%, MgSO 40.01-0.05%, KH 2PO 40.01-0.05%, urea 0.1-0.5%, V B10.002%; PH6.0-6.5; 25-35 ℃ of culture temperature, air quantity 1:0.1-10.5V/V, tank pressure 0.03-0.06, rotating speed 120-180rPm, 1-3 days time; The bacterium liquid that obtains is as liquid seed;
3. fermentation culture: by inoculum size 5-10% seed liquor that obtains is inoculated in fermentation cylinder for fermentation, fermention medium consists of: corn starch syrup 5-15%, MgSO 40.01-0.05%, KH 2PO 40.01-0.05%, CaCO 30.01-0.03%, urea 0.1-0.5%, V B10.002-0.005%; PH6.0-6.5, the same seeding tank of culture condition, incubation time 4-7 days;
4. microorganism collection and processing: after fermentation culture finishes, collect fresh thalli and dry.
Beneficial effect of the present invention:
1. compare with extract GLA from plant, the present invention has starting material and is easy to get, and technology is simple, and is with low cost, and be convenient to batch production production.GLA compares with other Production by Microorganism Fermentation, and the bacterial strain productive rate height that the present invention obtains can reach 30-39%;
2. after testing, except that containing abundant unsaturated fatty acids (mainly being GLA), also contain protein-polysaccharide, 18 kinds of indispensable amino acids, multivitamin (V in the dry bacterial powder that the present invention obtains B1, V B2, V B3, VC, VE etc.), superoxide-dismutase multiple nutritional components and biologically active substances such as (SOD), wherein fat content is 15-39%, GLA content is 10-30% in the lipid acid;
3. studies show that polysaccharide can improve the immunological competence of body, and have tissue repair, anti-inflammatory, effect such as antitumor; SOD is an important oxygen free radical scavenger in the body, can protect cell to avoid damage.The physiological function of comprehensive above two kinds of biologically active substances, the dry bacterial powder that is rich in GLA that the present invention obtains has important trophism and pharmacological action to human body, can be widely used in medicine, food, healthcare products and cosmetic field.
Embodiment:
Embodiment 1
1. bacterial classification expands numerous: freeze-drying is guaranteed the inoculation of depositing on the potato agar slant culture-medium, cultivated 4 days for 30 ℃ that add 0.1-0.2% tween 80 solution washing mycelium, making spore content is 1 * 10 5Individual spore suspension;
2. seed liquor prepares: the suspension for preparing is seeded in the seeding tank cultivates; Seed culture medium consists of: corn starch syrup (DE value 20) 2%, MgSO 40.02%, KH 2PO 40.025%, urea 0.1%, V B10.002%; PH6.0-6.5; 30 ℃ of culture temperature, air quantity 1:0.1V/V, tank pressure 0.03, rotating speed 120rPm, 2 days time; The bacterium liquid that obtains is as liquid seed;
3. fermentation culture: the seed liquor that obtains is inoculated in fermentation cylinder for fermentation by inoculum size 5%; Fermention medium consists of: corn starch syrup (DE value 20) 5%, MgSO 40.02%, KH 2PO 40.025%, CaCO 30.01%, urea 0.1%, V B10.002%; PH6.0; The same seeding tank of culture condition, incubation time 4 days;
4. microorganism collection and processing: after fermentation culture finished, centrifuging was collected fresh thalli, and the wash-out after drying makes its water content not be higher than 15%, refrigerates standby afterwards; The dry bacterial powder fat content is 27.4%, and GLA content is 20.7% in the lipid acid.
Embodiment 2
1. bacterial classification expands numerous: freeze-drying is guaranteed the inoculation of depositing on the potato agar slant culture-medium, cultivated 4 days for 30 ℃ that add 0.1-0.2% tween 80 solution washing mycelium, making spore content is 1.5 * 10 5Individual spore suspension;
2. seed liquor prepares: the suspension for preparing is seeded in the seeding tank cultivates; Seed culture medium consists of: corn starch syrup (DE value 40) 3%, MgSO 40.03%, KH 2PO 40.03%, urea 0.15%, V B10.002%; PH6.0; 30 ℃ of culture temperature, air quantity 1:0.3V/V, tank pressure 0.05, rotating speed 160rPm, 2 days time; The bacterium liquid that obtains is as liquid seed;
3. fermentation culture: the seed liquor that obtains is inoculated in fermentation cylinder for fermentation by inoculum size 5%; Fermention medium consists of: corn starch syrup (DE value 40) 7%, MgSO 40.03%, KH 2PO 40.03%, CaCO 30.02%, urea 0.15%, V B10.002%; PH6.0; The same seeding tank of culture condition, incubation time 5 days;
4. microorganism collection and processing: after fermentation culture finished, centrifuging was collected fresh thalli, and the wash-out after drying makes its water content not be higher than 15%, refrigerates standby afterwards; The dry bacterial powder fat content is 35.3%, and GLA content is 27.0% in the lipid acid.
Embodiment 3
1. bacterial classification expands numerous: freeze-drying is guaranteed the inoculation of depositing on the potato agar slant culture-medium, cultivated 5 days for 30 ℃ that add 0.1-0.2% tween 80 solution washing mycelium, making spore content is 1.5 * 10 5Individual spore suspension;
2. seed liquor prepares: the suspension for preparing is seeded in the seeding tank cultivates; Seed culture medium consists of: corn starch syrup (DE value 50) 3%, MgSO 40.03%, KH 2PO 40.03%, urea 0.5%, V B10.002%; PH6.2; 30 ℃ of culture temperature, air quantity 1:0.5V/V, tank pressure 0.05, rotating speed 180rPm, 3 days time; The bacterium liquid that obtains is as liquid seed;
3. fermentation culture: the seed liquor that obtains is inoculated in fermentation cylinder for fermentation by inoculum size 10%; Fermention medium consists of: corn starch syrup (DE value 50) 9%, MgSO 40.03%, KH 2PO 40.03%, CaCO 30.03%, urea 0.5%, V B10.005%; PH6.0; The same seeding tank of culture condition, incubation time 6 days;
4. microorganism collection and processing: after fermentation culture finished, centrifuging was collected fresh thalli, and the wash-out after drying makes its water content not be higher than 15%, refrigerates standby afterwards; The dry bacterial powder fat content is 37.3%, and GLA content is 28.5% in the lipid acid.
Embodiment 4
1. bacterial classification expands numerous: freeze-drying is guaranteed the inoculation of depositing on the potato agar slant culture-medium, cultivated 5 days for 30 ℃ that add 0.1-0.2% tween 80 solution washing mycelium, making spore content is 2 * 10 5Individual spore suspension;
2. seed liquor prepares: the suspension for preparing is seeded in the seeding tank cultivates; Seed culture medium consists of: corn starch syrup (DE value 70) 2%, MgSO 40.02%, KH 2PO 40.02%, urea 0.2%, V B10.002%; PH6.2; 30 ℃ of culture temperature, air quantity 1:0.5V/V, tank pressure 0.05, rotating speed 180rPm, 3 days time; The bacterium liquid that obtains is as liquid seed;
3. fermentation culture: the seed liquor that obtains is inoculated in fermentation cylinder for fermentation by inoculum size 5%; Fermention medium consists of: corn starch syrup 5%, MgSO 40.02%, KH 2PO 40.02%, CaCO 30.02%, urea 0.3%, V B10.002%; PH6.2; The same seeding tank of culture condition, incubation time 6 days;
4. microorganism collection and processing: after fermentation culture finished, centrifuging was collected fresh thalli, and the wash-out after drying makes its water content not be higher than 15%, refrigerates standby afterwards; The dry bacterial powder fat content is 38.7%, and GLA content is 27.9% in the lipid acid.

Claims (5)

1, be the zymotechnique of main material production gamma-linolenic acid with the corn starch syrup, it is characterized in that, adopt following technical scheme:
(1) high-yield strains obtains
With cunninghamella echinulata (formal name used at school Cunninghamella echinulata, the microbial strains preservation center preservation of country of institute of microbiology of the Chinese Academy of Sciences, preserving number: AS3.2473) separate spore after microwave treatment,, obtain superior strain again through ultraviolet mutagenesis; This bacterial strain initial stage on the potato substratum is white cotton wool shape, after gradually become lark, the flourishing racemosus of mycelium; Compare with original strain, fast growth, the spore amount is many, and fermentation back bacterium powder yield can reach 30～39g/L;
(2) gamma-linolenic acid is produced the zymotechnique flow process
1. bacterial classification expands numerous: making spore content is 1-2 * 10 5Individual spore suspension;
2. seed liquor prepares: the suspension for preparing is seeded in the seeding tank cultivates; Seed culture medium consists of: corn starch syrup 2-5%, MgSO 40.01-0.05%, KH 2PO 40.01-0.05%, urea 0.1-0.5%, V B10.002%; PH6.0-6.5; 25-35 ℃ of culture temperature, air quantity 1:0.1-1:0.5V/V, tank pressure 0.03-0.06, rotating speed 120-180rPm, 1-3 days time; The bacterium liquid that obtains is as liquid seed
3. fermentation culture: by inoculum size 5-10% seed liquor that obtains is inoculated in fermentation cylinder for fermentation, fermention medium consists of: corn starch syrup 5-15%, MgSO 40.01-0.05%, KH 2PO 40.01-0.05%, CaCO 30.01-0.03%, urea 0.1-0.5%, V B10.002-0.005%; PH6.0-6.5; The same seeding tank of culture condition, incubation time 4-7 days;
4. microorganism collection and processing: after fermentation culture finishes, collect fresh thalli and dry.
2, according to claim 1 is the zymotechnique of main material production gamma-linolenic acid with the corn starch syrup, it is characterized in that, gamma-linolenic acid is produced the zymotechnique flow process and adopted following technical scheme:
1. bacterial classification expands numerous: freeze-drying is guaranteed the inoculation of depositing on the potato agar slant culture-medium, cultivated 4 days for 30 ℃ that add 0.1-0.2% tween 80 solution washing mycelium, making spore content is 1 * 10 5Individual spore suspension;
2. seed liquor prepares: the suspension for preparing is seeded in the seeding tank cultivates; Seed culture medium consists of: corn starch syrup (DE value 20) 2%, MgSO 40.02%, KH 2PO 40.025%, urea 0.1%, V B10.002%; PH6.0-6.5; 30 ℃ of culture temperature, air quantity 1:0.1V/V, tank pressure 0.03, rotating speed 120rPm, 2 days time; The bacterium liquid that obtains is as liquid seed;
3. fermentation culture: the seed liquor that obtains is inoculated in fermentation cylinder for fermentation by inoculum size 5%; Fermention medium consists of: corn starch syrup (DE value 20) 5%, MgSO 40.02%, KH 2PO 40.025%, CaCO 30.01%, urea 0.1%, V B10.002%; PH6.0; The same seeding tank of culture condition, incubation time 4 days;
4. microorganism collection and processing: after fermentation culture finished, centrifuging was collected fresh thalli, and the wash-out after drying makes its water content not be higher than 15%, refrigerates standby afterwards; The dry bacterial powder fat content is 27.4%, and GLA content is 20.7% in the lipid acid.
3, according to claim 1 is the zymotechnique of main material production gamma-linolenic acid with the corn starch syrup, it is characterized in that, gamma-linolenic acid is produced the zymotechnique flow process and adopted following technical scheme:
1. bacterial classification expands numerous: freeze-drying is guaranteed the inoculation of depositing on the potato agar slant culture-medium, cultivated 4 days for 30 ℃, add 0.1-0.2% tween 80 solution washing mycelium, make spore content and be 1.5 * 10 spore suspension;
2. seed liquor prepares: the suspension for preparing is seeded in the seeding tank cultivates; Seed culture medium consists of: corn starch syrup (DE value 40) 3%, MgSO 40.03%, KH 2PO 40.03%, urea 0.15%, V B10.002%; PH6.0; 30 ℃ of culture temperature, air quantity 1:0.3V/V, tank pressure 0.05, rotating speed 160rPm, 2 days time; The bacterium liquid that obtains is as liquid seed;
3. fermentation culture: the seed liquor that obtains is inoculated in fermentation cylinder for fermentation by inoculum size 5%; Fermention medium consists of: corn starch syrup (DE value 40) 7%, MgSO 40.03%, KH 2PO 40.03%, CaCO 30.02%, urea 0.15%, V B10.002%; PH6.0; The same seeding tank of culture condition, incubation time 5 days;
4. microorganism collection and processing: after fermentation culture finished, centrifuging was collected fresh thalli, and the wash-out after drying makes its water content not be higher than 15%, refrigerates standby afterwards; The dry bacterial powder fat content is 35.3%, and GLA content is 27.0% in the lipid acid.
4, according to claim 1 is the zymotechnique of main material production gamma-linolenic acid with the corn starch syrup, it is characterized in that, gamma-linolenic acid is produced the zymotechnique flow process and adopted following technical scheme:
1. bacterial classification expands numerous: freeze-drying is guaranteed the inoculation of depositing on the potato agar slant culture-medium, cultivated 5 days for 30 ℃ that add 0.1-0.2% tween 80 solution washing mycelium, making spore content is 1.5 * 10 5Individual spore suspension;
2. seed liquor prepares: the suspension for preparing is seeded in the seeding tank cultivates; Seed culture medium consists of: corn starch syrup (DE value 50) 3%, MgSO 40.03%, KH 2PO 40.03%, urea 0.5%, V B10.002%; PH6.2; 30 ℃ of culture temperature, air quantity 1:0.5V/V, tank pressure 0.05, rotating speed 180rPm, 3 days time; The bacterium liquid that obtains is as liquid seed;
3. fermentation culture: the seed liquor that obtains is inoculated in fermentation cylinder for fermentation by inoculum size 10%; Fermention medium consists of: corn starch syrup (DE value 50) 9%, MgSO 40.03%, KH 2PO 40.03%, CaCO 30.03%, urea 0.5%, V B10.005%; PH6.0; The same seeding tank of culture condition, incubation time 6 days;
4. microorganism collection and processing: after fermentation culture finished, centrifuging was collected fresh thalli, and the wash-out after drying makes its water content not be higher than 15%, refrigerates standby afterwards; The dry bacterial powder fat content is 37.3%, and GLA content is 28.5% in the lipid acid.
5, according to claim 1 is the zymotechnique of main material production gamma-linolenic acid with the corn starch syrup, it is characterized in that, gamma-linolenic acid is produced the zymotechnique flow process and adopted following technical scheme:
1. bacterial classification expands numerous: freeze-drying is guaranteed the inoculation of depositing on the potato agar slant culture-medium, cultivated 5 days for 30 ℃ that add 0.1-0.2% tween 80 solution washing mycelium, making spore content is 2 * 10 5Individual spore suspension;
2. seed liquor prepares: the suspension for preparing is seeded in the seeding tank cultivates; Seed culture medium consists of: corn starch syrup (DE value 70) 2%, MgSO 40.02%, KH 2PO 40.02%, urea 0.2%, V B10.002%; PH6.2; 30 ℃ of culture temperature, air quantity 1:0.5V/V, tank pressure 0.05, rotating speed 180rPm, 3 days time; The bacterium liquid that obtains is as liquid seed;
3. fermentation culture: the seed liquor that obtains is inoculated in fermentation cylinder for fermentation by inoculum size 5%; Fermention medium consists of: corn starch syrup 5%, MgSO 40.02%, KH 2PO 40.02%, CaCO 30.02%, urea 0.3%, V B10.002%; PH6.2; The same seeding tank of culture condition, incubation time 6 days;
4. microorganism collection and processing: after fermentation culture finished, centrifuging was collected fresh thalli, and the wash-out after drying makes its water content not be higher than 15%, refrigerates standby afterwards; The dry bacterial powder fat content is 38.7%, and GLA content is 27.9% in the lipid acid.
CNA2007101583943A 2007-11-20 2007-11-20 Fermentation process for producing gamma-linolenic acid with corn starch syrup as principal raw material Pending CN101440383A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNA2007101583943A CN101440383A (en) 2007-11-20 2007-11-20 Fermentation process for producing gamma-linolenic acid with corn starch syrup as principal raw material

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNA2007101583943A CN101440383A (en) 2007-11-20 2007-11-20 Fermentation process for producing gamma-linolenic acid with corn starch syrup as principal raw material

Publications (1)

Publication Number Publication Date
CN101440383A true CN101440383A (en) 2009-05-27

Family

ID=40724993

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA2007101583943A Pending CN101440383A (en) 2007-11-20 2007-11-20 Fermentation process for producing gamma-linolenic acid with corn starch syrup as principal raw material

Country Status (1)

Country Link
CN (1) CN101440383A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102676594A (en) * 2012-05-25 2012-09-19 厦门大学 Preparation method of gamma-linolenic acid
CN111961693A (en) * 2020-05-21 2020-11-20 柯邦生物科技(上海)有限公司 New application of Cordyceps militaris and new method for producing linoleic acid and gamma-linolenic acid by using Cordyceps militaris

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102676594A (en) * 2012-05-25 2012-09-19 厦门大学 Preparation method of gamma-linolenic acid
CN111961693A (en) * 2020-05-21 2020-11-20 柯邦生物科技(上海)有限公司 New application of Cordyceps militaris and new method for producing linoleic acid and gamma-linolenic acid by using Cordyceps militaris

Similar Documents

Publication Publication Date Title
CN101543285B (en) Wheat-bran dietary fiber composite functional fungus powder product and preparation process thereof
CN106834368A (en) A kind of method that utilization lignocellulose for fermentation produces L lactic acid
CN102925502A (en) Industry method for producing arachidonic acid grease by using mortierella alpine
CN105875198B (en) A kind of cultural method improving Cordyceps militaris spawn stability
CN102351580B (en) Method for preparing black fungus nutrient solution by utilizing food processing residues
CN104845896B (en) Produce the bacterial strain and method of Weilan gum
CN101736033A (en) Method for producing red yeast rice with functions of regulating lipoid and reducing blood pressure through submerged fermentation
CN101933439A (en) Method for improving phellinus igniarius hypha amount of submerged culture by utilizing plant oil
CN102948325A (en) Cordyceps militaris efficient quick cultivation technology
CN101658102B (en) Cultivating method of pleurotus ferulae
CN101513238B (en) Solid-state fermentation preparation method of lotus root dietary fiber and products thereof
CN101979616B (en) Method for producing erythritol by using broken rice
CN104302758B (en) Produce turanose bacterial strain and application thereof
CN102234670B (en) Method for producing bacterial cellulose through solid state fermentation by using inert adsorption carrier
CN101440383A (en) Fermentation process for producing gamma-linolenic acid with corn starch syrup as principal raw material
CN101497902B (en) Process for preparing microbe oil
CN102329833A (en) Method for producing sophorose ester through fermenting waste molasses and waste glycerin
ES2845632T3 (en) A selective microbial production of xylitol from a biomass-based sugar stream with an enriched pentose component
CN112143770B (en) Marine rhodotorula and application thereof in producing beta-carotene by taking straw as raw material
CN104774880A (en) Preparation method of L-lactic acid by fermenting sweet sorghum straw juice
CN103849575A (en) Production method of single-cell protein
CN103859151A (en) Production technology of bacillus natto fermented soybean meal
CN101979624B (en) Preparation method of microbial oil rich in gamma-linolenic acid
KR20090090855A (en) Large-scale production of b-glucan through semi-continuous fermentation performed with sparassis crispa mycelia
CN104862263A (en) Culture medium containing corn straws, preparation method of culture medium and method for culturing bacillus subtilis (or lactobacillus plantarum) by virtue of culture medium

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
C53 Correction of patent for invention or patent application
CB03 Change of inventor or designer information

Inventor after: Jin Suying

Inventor after: Yang Jiancheng

Inventor after: Pang Shuyan

Inventor before: Jin Suying

Inventor before: Yang Jiancheng

COR Change of bibliographic data

Free format text: CORRECT: INVENTOR; FROM: JIN SUYING YANG JIANCHENG TO: JIN SUYING YANG JIANCHENG PANG SHUYAN

SE01 Entry into force of request for substantive examination
C12 Rejection of a patent application after its publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20090527