CN1105807A - Method of liquid ferment for producing Jichongjun mycelium - Google Patents

Abstract

This liquid ferment method for producing collybia albuminosa mycelium includes such technological steps as collecting wild mycelium of collybia albuminosa, slant culture of sporophores in PAD medium, continuous passages, deep liquid culture after mycelium is fully activated, fermentation, filter, drying and breaking to obtain finished product that contains protein, amino acids, fungus polyose, glycopeptide, coumarin, phytosterol, and triterpenoid and may be used as food, medicine and feed additive. The filtering liquid (fermented one) may be used to prepare nutritive or fermented beverage.

Description

Method of liquid ferment for producing Jichongjun mycelium

The invention belongs to food and pharmaceutical raw material product and processing method thereof, specifically chicken

The mycelial fermentation method for producing of bacterium and products thereof formula development.

Chicken

[Termitomyces albuminosus (Berk) Heim] another name chicken silk mushroom, chicken bacterium, handle of umbrella mushroom, three altar bacterium.Belong to the fungi Basidiomycetes, Agaricales, white mushroom section, the long handle mushroom belongs to.Be distributed in ground such as China Jiangsu, Fujian, Guangdong, Guangxi, Sichuan, Yunnan, Guizhou.The delicious U.S. of this bacterial context, the protein content height, nutritious, be the wild delicious edible mushroom that a kind of common people of firmly getting like.Chicken

Also have high medical value, as far back as China Ming Dynasty Li Shizhen (1518-1593 A.D.)＜Compendium of Materia Medica〉and its reputation bun＜longevity generation secret allusion quotation of Qing Dynasty's fourth on just record: the effect of " the sweet clear god of the beneficial stomach of nontoxic master of putting down of smell controls hemorrhoid ".But the precious edible mushroom of the preciousness of this food medicine dual-purpose food medicine dual-purpose can only wild (with symbiosis of termite), can not propagate artificially, thereby only depend on field acquisition to obtain chicken The mushroom entity, not only output is limited, cost an arm and a leg, and the time short (annual acquisition time only 2～3 months).A large amount of chickens are produced in commercialization

Bacterium is to wait the difficult problem that solves at present.

Existing medicinal fungi (as glossy ganoderma, Hericium erinaceus, armillaria mellea etc.) obtained a large amount of mycelial methods and also uses fermenting and producing, but the cycle is long, as Hericium erinaceus fermentation period at least 7～9 days, glossy ganoderma 5～7 days, mushroom 2～3 days yielded poorly in the unit interval, and the possibility height of living contaminants.Overseas utilization yeast manufacture order cell protein, though the cycle is short, obtain dry matter needs centrifugal treating cumbersome, and during energy charge.

The objective of the invention is to wild chicken

Bacterium is used the liquid deep layer fermenting mode, suitability for industrialized production edible and medicinal chicken

Bacterium mycelium and zymotic fluid, and make the main material production several prods with this.

The major technique content is: bacterial strain separates, purifying; Deep liquid is cultivated (shaking bottle); Fermentation tank is produced chicken

The bacterium mycelium.Process is: the field acquisition chicken Bacterium is got a small pieces chicken with aseptic manipulation

Fruit body is after PAD medium ramp test tube is cultivated, treated that mycelium grows, continuous passage, guarantor plant, and mycelium is activation fully on solid culture medium, rapidly after the breeding, select the different liquids medium to carry out deep liquid again and cultivate, change fermentation tank then over to and produce chicken

The bacterium mycelium.Cultural method is as follows:

1. technological process and specification requirement:

Fruit body is dug piece at 28 ℃, and on 28 ℃ slant medium, the formula of being rotated is shaken bottle, after 48 hours, dig piece again in the seed bottle, ratio in 4～5%, at 28 ℃, shake bottle through 24 hours seeds, enter first class seed pot, ratio in 2%, at 28 ℃, the gap by 1: 0.5～1, stirred fermentor filters out wet mycelium then, is dried mycelium 50～60 ℃ of oven dry, it is finished product that dried mycelium is pulverized, and the zymotic fluid that leaches processing is mixed with nutrient solution or fermented beverage.

2. culture medium prescription:

Maltose 1～5%, peptone 0.1～0.5%,

Dusty yeast 0.1～0.5%, soyabean cake 0.5～2.5%,

KH ₂PO ₄0.1～0.5%， M _gSO ₄0.05～0.3%，

Glucose 0.2～0.7%, bubble enemy 0.01～0.05%,

The PH nature begins to stir during the sterilization cooling.

Replacements such as potato, molasses, glucose, sucrose, cassava can be used in the C source of medium.Inorganic ammonia salt such as NH can be used in the N source ₄H ₂PO ₄, (NH ₄) SO ₄, NH ₄NO ₃Deng replacement.

3. fermentation condition:

Inoculum concentration 1～2.5%;

Throughput 1: 1.0～1: 1.5(v/v);

Stir each 2 minutes per hour 1～3 time;

24～29 ℃ of temperature;

23～30 hours cycles;

Put a jar PH6.4～7.0

Fermentation is finished after the zymotic fluid processing preparation that plate-frame filtering goes out can be made into fermented beverage, nutrient solution.Dried mycelium can be made into chicken

Bacterium tasty agents, medicine and food or food additives are as chicken

Bacterium sheet (or capsule) and chicken

The syrup similar drug.

Major advantage of the present invention is:

1. the dried mycelium powder of producing is of high nutritive value, and contains protein 49.2%, 18 seed amino acid 31.8%, and wherein essential amino acid 15.15%, can be directly as food, medicine or additive for farm animal feed.Filtrate can be made into nutrient solution, fermented beverage.

2. dried mycelium has great exploitation to be worth aspect medicinal, contains abundant fungi polysaccharide, glycopeptide, coumarin, phytosterol and triterpenes.Can extract specific drug anticancer and treatment hepatitis.

3. fermentation period is short, and the yield in unit time height is difficult for being bacterial contamination.

4. this bacterium only can obtain with plate-frame filtering, and is simple and easy and energy-conservation than the yeast mode of production.

5. make substrate with maltose or potato, raw material is easy to get, and cost is low.

6. the factory that can make eliminating contain (C, N) source sewage utilizes this bacterium fermenting and producing mycelium to purify water.

The processing preparation of embodiment 1. zymotic fluids:

Under aseptic condition, the bacterial classification of getting on the inclined-plane solid culture medium of activation is a small amount of, be inoculated in the conical flask of 2/3 volume of liquid medium, 160 rev/mins, 28 ℃, vibration, treat that mycelium covers with back (48 hours) and changes in the first class seed pot by 1%～2% inoculation, change the secondary seed jar after 25 hours over to, changed big fermentation tank (500 liters) again through 30 hours over to, ferment 24 hours then plate-frame filtering must do mycelium.Temperature rises to 60 ℃ and kept 24 hours then, makes its mycelium self-dissolving, 100 ℃ of deactivations 20 minutes.Residual residue is through twice removal of plate-frame filtering, the deodorization of liquid part activated carbon decolorizing, and filtration sterilization is made chicken

Fermented liquid stoste

Chicken Nutrient solution: (unit: grams per liter)

Vitamin C 0.5; Honey 50;

Calcium glycerophosphate 0.5; White fungus 0.5;

Ferrous sulfate 0.04; Chicken

Bacterium stoste 1000ml

Embodiment 2. chickens

Fermented beverage: (unit: grams per liter)

Sucrose 40; Oligosaccharide 10; Dark plum 0.5;

Sodium chloride 0.6; Potassium chloride 0.1; Sodium dihydrogen phosphate 0.1;

Sodium bicarbonate 0.1; Potassium dihydrogen phosphate 0.1;

Chicken Zymotic fluid stoste 25%; Water 75%

Embodiment 3. chickens

Tasty agents:

Chicken

Mycelium protein, chicken, fish, shrimp, and flavor enhancement such as pepper be mixed into by a certain percentage

Chicken Bacterium mycelium powder 80%; Chicken 9.0%;

Fish 9.5%; Shrimp 1%;

Pepper and other flavor enhancement 0.5%

Embodiment 4.

Chicken

Bacterium mycelium dry powder is directly made food additives

Embodiment 5. chickens

The bacterium capsule:

Vitamin C: 2mg/ grain; B ₆: the 0.5mg/ grain;

Trace element: zinc 0.01mg/ grain, selenium 0.002mg/ grain;

Chicken

Bacterium mycelium dry powder adds to total amount 0.5g/ grain;

Make capsule.

Claims (4)

1, a kind of production chicken

The feature of mycelial method of bacterium and goods thereof is: adopt deep liquid to cultivate behind bacterial strain separation, the purifying, fermentation tank is produced chicken

The bacterium mycelium must be done mycelium and zymotic fluid behind the plate-frame filtering.

2, by the said production of claim 1 chicken The feature of mycelial method of bacterium and goods thereof is: dried mycelium adds flavor enhancement and can be made into chicken Bacterium food, or directly make food additives.

3, by the said production of claim 1 chicken The feature of mycelial method of bacterium and goods thereof is: dried mycelium adds vitamin and sugar can be made into chicken

Bacterium capsule, chicken

The syrup similar drug.

4, by the said production of claim 1 chicken The feature of mycelial method of bacterium and goods thereof is: zymotic fluid adds vitamin C, sugar and sodium bicarbonate and makes fermented beverage or nutrient solution.