CN101228951B - Preparing method of biologic compound amylose nutrient fluid - Google Patents
Preparing method of biologic compound amylose nutrient fluid Download PDFInfo
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Abstract
The invention relates to a preparation method of a biological composite polysaccharide efficient nutrient solution. The invention comprises the steps of selecting a strain, preparing a nutrient, culturing a liquid spawn, generating a mycelium in a fermentor, filtrating the fermentation broth and generating the nutrient solution which is rich in microbial polysaccharide after concentration. The key of the invention lies in that the composite nutrient solution is made by mixing the mycelium culture medium extracted from the respective fermentor after deep fermentation of the following microbialstrains after the above steps according to the following weight proportion: mythic fungus of 5 to 20 percent, aweto of 5 to 20 percent, poria of 5 to 15 percent, grey polypore of 5 to 15 percent, boletus of 5 to 15 percent, phellinus linteus of 5 to 10 percent, many zoned polypore of 5 to 20 percent, mushroom of 6 to 20 percent, bailing of 5 to 10 percent mushroom and agaricus blazei strain of 5 to 20 percent. The nutrient solution is flask separated, sealed and sterilized under bacteria free environment to become the final product.
Description
Technical field
The invention belongs to a kind of preparation method of biologic compound amylose nutrient fluid with high efficiency.
Technical background
The mankind stride into 21 century, " healthy, the long-lived eternal theme that has become the human life ", yet the pollution of big in recent years industrialized process, atmosphere, water, food, and the quickening of rhythm of life, people's unhealthy emotion etc. has caused the generation of serial disease.To antitumor, end blood fat, blood sugar, prevention cardiovascular and cerebrovascular disease fall, become the challenge that the whole mankind faces jointly.Especially tumor patient is on the increase, and human beings'health has been subjected to very big threat.Declare a war to tumour and to launch in the whole world.
Bioactive polysaccharide (Biologically Active Polysaccharides is called for short BAP) is the polymeric carbohydrate condensate that a class has multiple physiologically active and special health care.General single glycan, heteroglycan or the mucopolysaccharide that forms with special glycosidic bond condensation by the monose more than one or more more than seven.Develop rapidly along with the medical science technology, to deepening continuously of the aspect researchs such as physicochemical property, active ingredient, preparation method, detection means, biologically active and the mechanism of action thereof of BAP, it is found that the effect that various BAP has adjusting in various degree respectively and improves immunity of organisms.For anti-oxidant, antifatigue, prolong silk ribbon attached to an official seal or a medal aging, antitumor, anti-sudden change, antirheumatic, radioresistance, anticoagulation, anti-inflammation, reducing blood lipid, hypotensive, hypoglycemic, cerebral thrombus, coronary heart disease, atherosclerotic, fatty liver, obesity, the diabetes of control cardiovascular and cerebrovascular disease, simultaneously can also promote marrow hemopoiesis, increasing leukocyte, blood platelet, improve blood picture, to simple property leukopenia, thrombopenia, alpastic anemia has role of correcting.At protection liver, renal function, raising hematological indices etc. has obvious effect.In a word, immunologic hypofunction and the damaged eqpidemic disease of panimmunity and various person in middle and old age's property disease had good adjuvant treatment effect and health function.Though no matter be that medicinal or practical microbial polysaccharide plays a significant role for the immunologic function and the element body mass-energy that improve the people, act on single, and not lasting.That is to say that its effect can only temporarily solve in the blood improvement of abnormal index in a certain respect, can not thoroughly improve the index that all exceeds standard in the blood.Other complementary effect more out of the question.When can reduce blood cholesterol, triglycerides, blood viscosity, blood sugar fully and effectively, play the nutrition softening blood vessel again, replenish vascular wall nutrition, the complex polysaccharide series products that improves the human body comprehensive immunologic function does not occur at present as yet.
Further discover the complex polysaccharide that the multiple microbial polysaccharide of obvious health-care effect forms after mixing, health-care effect is several times, and tens times of ground improve.Complex polysaccharide has many-sided biologically active.Be high-quality and immunomodulator without any side effects in the world today, can promote body immunity effectively comprehensively, comprising the important component that must want in human body cell and the organ composition again, is that human body cell or internal organs are kept the indispensable nutritional supplementation of normal function.Polysaccharide is listed in the anticancer of remarkable result in the world, immunopotentiator and interferon inducers.
Summary of the invention
The purpose of this invention is to provide a kind of method of producing with the biologic compound amylose high efficiency nutrient solution that is main component by microorganism submerged fermentation technology.Antitumor with the class reply of getting married, the needs to the high-efficient health-care product fall in end blood fat, blood sugar, the prevention cardiovascular and cerebrovascular disease.
The present invention is a kind of preparation method of biologic compound amylose nutrient fluid with high efficiency, comprise in this method mycelial generation in the cultivation, fermentation tank of preparation, the liquid spawn of the choosing of bacterial classification, nutrition base, zymotic fluid filtration, concentrate the back and generate the nutrient solution that is rich in microbial polysaccharide, crucial technology is that said composite nutrient-fluid is by producing to such an extent that be mixed after the nutrient solution and form more than a kind of microorganism fungus kind through above method in this method, and concrete steps are as follows:
A; choose glossy ganoderma (Lucudium Ganoderma) respectively; Cordyceps sinensis (MilitrisGordyceps); Poria cocos (Cocos Poria); grifola frondosus (Frondons Grifola); bolete (Edulis Boletus); Phellinus (Igniarius Phellinus); rainbow conk (VersicolorConorus); mushroom (Edodus Lentinula); Pleurotus nebrodensis (Nebrodensis Pleurotus); the bacterial classification of Agaricus blazei (Bispocus Agaricus); take slant strains to cultivate; the breeding of shaking table liquid; seeding tank liquid spawn three-class strain is cultivated liquid spawn separately; liquid spawn being introduced supporting fermentation tank respectively fermented 3-7 days in supporting liquid culture medium again; treat that mycelial growth concentration or weight in wet base ratio stops fermentation when reaching 65-80%; solid residue in the filtering fermentating liquid; it is stand-by to obtain the corresponding strain fermentation filtered solution of institute
B, in temperature is not less than under 90 ℃, an environmental pressure 1.2-1.5 atmospheric pressure above each strain fermentation filtered solution, add 0.5-1.5%, by etc. carry out pyrolysis, inactivation treatment behind the pepsin of weight and the compound protease that papain is formed,
C, will further filter, concentrate to remove moisture content and the foreign material of 10%-15% through each strain fermentation filtered solution of pyrolysis, inactivation treatment,
D, then the above said extract that is made by each bacterial classification is mixed into composite nutrient-fluid according to following percent by volume:
Glossy ganoderma: 5-20%, Cordyceps sinensis: 5-20%,
Poria cocos: 5-15%, grifola frondosus: 5-15%,
Bolete: 5-15%, Phellinus: 5-10%,
Rainbow conk: 5-20%, mushroom: 6-20%,
Pleurotus nebrodensis: 5-10%, Agaricus blazei: 5-20%,
E, under gnotobasis, carry out the branch bottle, encapsulation, sterilization becomes finished product.
Selected glossy ganoderma, Cordyceps sinensis, Poria cocos, grifola frondosus, bolete, Phellinus, rainbow conk, mushroom, Pleurotus nebrodensis, Ji Songrong all is nontoxic edible mushroom or medicinal bacterial classifications among the present invention.It is the perfect combination that process inventor's long term studies and contrast repeatedly screen.The mycelium of various microorganisms carries out degree of depth fermentation process in fermentation tank, when weight in wet base reached 65-85%, the effective content of its polysaccharide produced significantly and improves, its nourishing and medicinal effects can reach and extremely.Effect makes its nourishing function and drug effect strengthen mutually between the complex polysaccharide.
The biologic compound amylose that this method is produced proves after testing and is rich in iron, copper and vitamin C.Organic selenium that is rich in and chromium have protection liver, pancreas, prevention anaemia, scurvy, cirrhosis effect.Complex polysaccharide BAP in this product of pathology description of test can make fatty liver liver tissues of rats triglycerides due to the CC14, and T-CHOL and free fatty acid content significantly descend, and hepatic tissue cell fat range degree obviously slows down, and fatty liver is had fine protective effect.The complex polysaccharide molecular weight reaches 9,000,000 in this product after testing, and main antineoplastic component such as nucleic acid, organic selenium, Organic Chromium, organic zinc, organic germanium contains 36 kinds of trace elements of higher nutrient, 13 seed amino acids, multivitamin.Have that tonification and purgation in combination, effect such as balanced in nutrition, across-the-board regulation, raising body's immunity.The product that clinical trial has proved this method manufacturing to antitumor, end blood fat, blood sugar fall, the prevention cardiovascular and cerebrovascular disease all has tangible curative effect, is the health product that has application prospect most at present.
The specific embodiment
The present invention is a kind of preparation method of biologic compound amylose nutrient fluid with high efficiency, comprise in this method mycelial generation in the cultivation, fermentation tank of preparation, the liquid spawn of the choosing of bacterial classification, nutrition base, zymotic fluid filtration, concentrate the back and generate the nutrient solution that is rich in microbial polysaccharide, key technology is in this method that said composite nutrient-fluid is by producing to such an extent that be mixed after the nutrient solution and form more than a kind of microorganism fungus kind through above method, and concrete steps are as follows:
A; choose glossy ganoderma (Lucudium Ganoderma) respectively; Cordyceps sinensis (MilitrisGordyceps); Poria cocos (Cocos Poria); grifola frondosus (Frondons Grifola); bolete (Edulis Boletus); Phellinus (Igniarius Phellinus); rainbow conk (VersicolorConorus); mushroom (Edodus Lentinula); Pleurotus nebrodensis (Nebrodensis Pleurotus); the bacterial classification of Agaricus blazei (Bispocus Agaricus); take slant strains to cultivate; the breeding of shaking table liquid; seeding tank liquid spawn three-class strain is cultivated liquid spawn separately; liquid spawn being introduced supporting fermentation tank respectively fermented 3-7 days in supporting liquid culture medium again; treat that mycelial growth concentration or weight in wet base ratio stops fermentation when reaching 65-80%; solid residue in the filtering fermentating liquid; it is stand-by to obtain the corresponding strain fermentation filtered solution of institute
B, in temperature is not less than under 90 ℃, an environmental pressure 1.2-1.5 atmospheric pressure above each strain fermentation filtered solution, add 0.5-1.5%, by etc. carry out pyrolysis, inactivation treatment behind the pepsin of weight and the compound protease that papain is formed,
C, will further filter, concentrate to remove moisture content and the foreign material of 10%-15% through each strain fermentation filtered solution of pyrolysis, inactivation treatment,
D, then the above said extract that is made by each bacterial classification is mixed into composite nutrient-fluid according to following percent by volume:
Glossy ganoderma: 5-20%, Cordyceps sinensis: 5-20%,
Poria cocos: 5-15%, grifola frondosus: 5-15%,
Bolete: 5-15%, Phellinus: 5-10%,
Rainbow conk: 5-20%, mushroom: 6-20%,
Pleurotus nebrodensis: 5-10%, Agaricus blazei: 5-20%,
E, under gnotobasis, carry out the branch bottle, encapsulation, sterilization becomes finished product.
Percent by volume is mixed into composite nutrient-fluid embodiment:
More than the process conditions grasped in said fermentation or the breeding process be that fermentation temperature 24-26 ℃, the fermentation tank pressure is 30-50KPa, culture medium carbon, nitrogen ratio are 1: 16-1: 24, inoculum concentration is 10%, and throughput amount 0.4-0.6 cubic meter/cubic meter divides, mixing speed 120-180 rev/min.
More than in the preparation method of said biologic compound amylose nutrient fluid with high efficiency in the supporting above step of taking the liquid culture medium in fermentation or the breeding jar be according to the preparation of following percentage by weight:
The liquid culture medium proportioning of glossy ganoderma, Poria cocos, grifola frondosus, Phellinus, rainbow conk in a, this method:
Murphy juice 20-30
Soya-bean milk 25-35
Millet juice 5-15
Corn juice 10-15
Dusty yeast 0.5-1
Multitudinous sugared 0.5-3
Glucose 0.5-2
Peptone 0.3-1.5
Agar 0.5-1
KH
2PO
4 0.1-0.3
K
2HPO
4 0.05-0.15
Mnso
4 0.2-0.5
Caco
3 0.5-0.8
Znso
4 0.2-0.4
Mncl
2 0.1-0.3
Cobastab 10.1-1
Peanut oil 0.3-0.5
Water surplus
The liquid culture medium proportioning of b, bolete, mushroom, Pleurotus nebrodensis, Ji Songrong:
The liquid culture medium proportioning of C, Cordyceps sinensis:
Dried silkworm chrysalis meal 1-2,
Milk powder 2-3
Culture medium proportioning embodiment (percentage by weight)
More than in the fermentation or the liquid culture medium layoutprocedure in the breeding jar of preparation method of said biologic compound amylose nutrient fluid with high efficiency, with peeling potatoes and eye, section adds 6-9 times of weight water, boil to stir and be pasty state, corn flour adds 25-30 times of weight water boil, millet kept 30 minutes after adding 30-40 times of weight water boil, make 100 jin of soya-bean milk with 3-6 jin soya bean, above semi-finished product form said potato juice through getting filtrate with 6-8 layer filtered through gauze, corn flour juice, millet juice, soya-bean milk, accurately take by weighing required various compound according to the above proportion compatibility that provides then, adding agar at last stirs to dissolving fully, carry out steam high-voltage sterilizing at last, be cooled to below 30 ℃ standby.
Prepared composite nutrient-fluid is further made pulvis by spray-drying in the steps d of the preparation method of above biologic compound amylose nutrient fluid with high efficiency, makes compound polysaccharide capsule or electuary or tablet again.
Prepared composite nutrient-fluid is that the employing milipore filter separates concentrated or the reverse osmosis membrane purifying concentrates and the complex polysaccharide nutrient solution of acquisition in the above step.
The inventor has confirmed the good effect of the product that use this method is made by further experiment detection and clinical practice:
1, anti-tumor function:
During the pharmacological evaluation mouse cooked with the nutrient solution drug effect of this method manufacturing, pour into complex polysaccharide for mouse chamber abdomen, metastatic tumo(u)r is had remarkable inhibitory action, and can suppress metastases and growth according to the injection rate of 4-6 gram/kilogram.Complex polysaccharide has better curative effect to people's stomach oncocyte SGC-790 and people's carcinoma of the rectum CO10-205, can stop that the SO/G1 phase enters the S phase in the SGC-790 gastric carcinoma cells, and the rising cancer cell index that withers, inducing cancer cell access and form antitumaous effect.
2, anti-oxidation function
Complex polysaccharide can significantly reduce MDA in the mouse tissue (MDA) content, promote the activity of SOD in the tissues such as cell, liver, brain, the activation recovering that can make the acid of the sweet attitude peroxide of hepatic tissue two-story valley gastral cavity has strengthened the ability that body is anti-oxidant and Green Tea Extract damages to normal level.Complex polysaccharide can form one deck sugar barrier at endothelial cell surface, and the integrality of diaphragm structure has prevented that the merit of oxygen radical from hitting.Also can form relevant metal ion and combine, suppress the generation of OH, also can reduce MDA content in the mitochondria, subtract the liver lipid peroxidation degree, improve the mitochondrial membrane flowability, remove aliphatic acid β one dysoxidation and produce antioxidation with OH.
3, anti-cardiovascular disease function:
Complex polysaccharide has obvious reduction high fat of blood and increasing high density lipoprotein effect.Its mechanism is that polysaccharide can combine with the lipid material in the serum, participated in the metabolic activity of cholesterol, lipoprotein as a kind of carrier, quickened the transhipment and the drainage of lipid material, and can make cholesterol be oxidized to cholic acid quickly, influences its metabolic pathway.BAP can significantly increase the activity of being tried rabbit anticoagulation kinase, prolong the clotting time, suppress the formation of thrombus, can significantly suppress the activity of platelet activating factor, suppressed the platelet aggregation that collagen and arachidonic acid are induced, improved microcirculation, quickened blood flow, prevented thrombosis, it is super normal to make the blood mobility learn index, thereby heart stalk, cerebral infarction have been prevented, the generation of cardiovascular and cerebrovascular diseases such as coronary sclerosis.
4, anti-fatigue effect
Give the mouse stomach complex polysaccharide, every 10mg/kg.d, continuous 10 days, found that compare with control group, this sugar can significantly increase the mouse muscle glycogen, the hepatic glycogen storage level significantly improves the glycogen recovery rate, provides material base to setting up after the large amount of exercise.Complex polysaccharide can significantly increase with the swimming death time of sawing Lung Qi deficiency model mice due to the not sootiness, has more significant anti-anoxic, antifatigue effect.
5, anti-inflammation function
The low-molecular-weight complex polysaccharide has direct inhibition pathogenic bacteria its pathological reaction function of growing in rodent, the peculiar suction moisture-keeping functions of large molecular weight polysaccharides, can easily form one deck hydration shell at skin surface, this film is as one natural cover for defense, can effectively skin and bacterium be kept apart, stop bacterium to invade skin inside, produced the anti-inflammation effect indirectly.In addition; in skin histology inside, polysaccharide and protein combination form hyaluronic acid; albumen composition is present in the space between cells; and formation high-molecular gel; with cell adhesion together; performance cell eubolism and organize the water holding effect produces the protective tissue cell and avoids the infringement of pathogen and protect from infection etc., so complex polysaccharide has anti-inflammation, antivirus action.
6, hypotensive hypoglycemic
Experimentize with spontaneous hypertensive rat, the administration group adds 5% complex polysaccharide in feed, and control group does not add, and the visible administration group blood pressure in 4 week backs obviously reduces, and the cholesterol level in blood plasma and the liver also descends.
This product proves by further toxicological test, does not contain anticorrisive agent.Through acute, subacute and chronic toxicity test, have no side effect.In the medicinal effects test report of product complex polysaccharide of the present invention with reference to the formulation principle of following criterion of therapeutical effect:
1, ((relevant efficacy assessment standard in the new Chinese medicine clinical guidance principle (being Nimodipine method { integration one is treated the back integration before the treatment }/integration before the treating) * 100% of WHO and the formulation of China Ministry of Public Health of the World Health Organization.
Produce effects: than descending before the medicine more than 2/3, the neurologic defect integration descends 〉=50% behind the tcm symptom integration medicine.
Effectively: Chinese medicine symptom integration decline 1/3-2/3, the neurologic defect integration descends＜20%.
2, tried the standard that relevant adjustment hypolipidemic medicine is studied and defined in the 15 class clinical drugs research guidelines (trying) with reference to the Ministry of Public Health.
Produce effects: reach following each person, TC descends 〉=20, and TG falls 〉=and 40, TDG-C risings 〉=10mg/dl, AL decline 〉=20%.
Effectively: reach following each person, TC decline 10-19%, TG decline 20-39%, HDC-C rising 1-9mc/dl, AL decline 10-20%.
Invalid: as not reach effective standard person:
Worsen: reach following each person TC and rise 〉=10%, TG rises 〉=10%, HDC-C decline 〉=4mg/dl, and AL rises 〉=10%.
Select the cardiovascular and cerebrovascular disease acute stage later 100 of convalescents be the contrast experiment, take this composite nutrient-fluid every day 4 times, each 20-40 milliliter, 10 days one-periods, carry out the detection and the Chinese medicine symptom efficacy evaluation of laggard line correlation index same period after four cycles:
Produce effects 76 examples, effective 18 examples, invalid 6 examples, total effective rate 94%.
Do not take the conventional therapy control group patient relevant evaluation data of this patent product:
Produce effects 6 examples, effective 29 examples, invalid 48 examples, total effective rate 42.16%.
The related neural functional impairment recovers efficacy evaluation:
The blood flow degree is learned the evaluation that influences of index:
P＜0.05 P＜0.01.
To celloglobulin, cell pack, erythrocyte electrophoretic time influence
P＜0.05 P＜0.01
Brief summary: two groups of contrast tcm symptoms are imitated evaluation before and after the medicine significant difference.Neurologic impairment recovers efficacy evaluation, and two groups have significant difference.Blood becomes the influence of learning index, behind the treatment group medicine, and whole blood contrast viscosity, plasma viscosity, the whole blood reduced viscosity, packed cell volume, celloglobulin, erythrocyte electrophoretic time all have remarkable reduction.
Hyperlipidemia:
Two groups of curative effects compare:
Variation to TC, TG, HDL-C, AL
P＜0.05 P＜0.01
Brief summary: there were significant differences in two groups of contrasts
TC, TG, Al all descend behind the medicine, and learning by statistics to handle has significant difference: HDC-C rises to some extent, but notable difference is arranged.
Bright by each above interview checking: the complex polysaccharide nutrient solution of this method made is as a kind of novel microbial enriched nutrient, the corresponding index that reduces in five of the blood fat there is fairly obvious effect, further analytical proof contains necessary nucleic acid, organic zinc, organic selenium, Organic Chromium, organic germanium trace element, vitamin etc. in certain rich in amino acid and vascular nutrition in this nutritional agents.Be a kind of balanced in nutrition, tonification and purgation in combination, the outstanding nutritional auxiliary of across-the-board regulation body's immunity.In the practice of pre-preventing tumor, antitumor, reducing blood lipid, prevention cardiovascular and cerebrovascular disease, be that human body cell or internal organs maintain the indispensable nutriment of normal function.Immunologic hypofunction and the damaged disease of panimmunity had good adjuvant treatment effect and health function.
Claims (6)
1. the preparation method of a biologic compound amylose nutrient fluid with high efficiency, comprise in this method mycelial generation in the cultivation, fermentation tank of preparation, the liquid spawn of the choosing of bacterial classification, nutrition base, zymotic fluid filtration, concentrate the back and generate the nutrient solution that is rich in microbial polysaccharide, it is characterized in that in this method that said composite nutrient-fluid is by producing to such an extent that be mixed after the nutrient solution and form more than a kind of microorganism fungus kind through above method, concrete steps are as follows:
A; choose glossy ganoderma (Lucudium Ganoderma) respectively; Cordyceps sinensis (Mi litri sGordyceps); Poria cocos (Cocos Poria); grifola frondosus (Frondons Grifola); bolete (Edulis Boletus); Phellinus (Igniarius Phellinus); rainbow conk (VersicolorConorus); mushroom (Edodus Lentinula); Pleurotus nebrodensis (Nebrodensis Pleurotus); the bacterial classification of Agaricus blazei (Bispocus Agaricus); take slant strains to cultivate; the breeding of shaking table liquid; seeding tank liquid spawn three-class strain is cultivated liquid spawn separately; liquid spawn being introduced supporting fermentation tank respectively fermented 3-7 days in supporting liquid culture medium again; treat that mycelial growth concentration or weight in wet base ratio stops fermentation when reaching 65-80%; solid residue in the filtering fermentating liquid; it is stand-by to obtain the corresponding strain fermentation filtered solution of institute
B, more than temperature is not less than under 90 ℃, an environmental pressure 1.2-1.5 atmospheric pressure just, add in each strain fermentation filtered solution 0.5-1.5%, by etc. carry out pyrolysis, inactivation treatment behind the pepsin of weight and the compound protease that papain is formed,
C, will further filter, concentrate to remove moisture content and the foreign material of 10%-15% through each strain fermentation filtered solution of pyrolysis, inactivation treatment,
D, then the above said extract that is made by each bacterial classification is mixed into composite nutrient-fluid according to following percent by volume:
Glossy ganoderma: 5-20%, Cordyceps sinensis: 5-20%,
Poria cocos: 5-15%, grifola frondosus: 5-15%,
Bolete: 5-15%, Phellinus: 5-10%,
Rainbow conk: 5-20%, mushroom: 6-20%,
Pleurotus nebrodensis: 5-10%, Agaricus blazei: 5-20%,
E, under gnotobasis, carry out the branch bottle, encapsulation, sterilization becomes finished product.
2. according to the preparation method of the said biologic compound amylose nutrient fluid with high efficiency of claim 1, it is characterized in that the process conditions of being grasped in above said fermentation or the breeding process are, fermentation temperature 24-26 ℃, the fermentation tank pressure is 30-50MPa, culture medium carbon, nitrogen ratio are 1: 16-24, inoculum concentration is 10%, and throughput amount 0.4-0.6 cubic meter/cubic meter divides, mixing speed 120-180 rev/min.
3. according to the preparation method of the said biologic compound amylose nutrient fluid with high efficiency of claim 1, it is characterized in that liquid culture medium in fermentation in the above step or the breeding jar is according to the preparation of following percentage by weight:
The liquid culture medium proportioning of glossy ganoderma, Poria cocos, grifola frondosus, Phellinus, rainbow conk in a, this method:
Murphy juice 20-30
Soya-bean milk 25-35
Millet juice 5-15
Corn juice 10-15
Dusty yeast 0.5-1
Multitudinous sugared 0.5-3
Glucose 0.5-2
Peptone 0.3-1.5
Agar 0.5-1
KH
2PO
4 0.1-0.3
K
2HPO
4 0.05-0.15
Mnso
4 0.2-0.5
Caco
3 0.5-0.8
Znso
4 0.2-0.4
Mncl
2 20.1-0.3
Vitamin B1 0.1-1
Peanut oil 0.3-0.5
Water surplus
The liquid culture medium proportioning of b, bolete, mushroom, Pleurotus nebrodensis, Ji Songrong:
Murphy juice 20-35
Soya-bean milk 20-30
Corn juice 5-10
Dusty yeast 0.5-1
Multitudinous sugared 0.5-2
Glucose 0.5-3
Peptone 0.3-1
Agar 0.5-1
KH
2PO
4 0.1-0.3
K
2HPO
4
Mnso
4 0.2-0.4
Caco
3
Znso
4 0.2-0.4
Mncl
2 0.1-0.3
Vitamin B1 0.1-1
Peanut oil 0.3-0.5
Water surplus
The liquid culture medium proportioning of C, Cordyceps sinensis:
Dried silkworm chrysalis meal 1-2,
Milk powder 2-3
Murphy juice 20-30
Soya-bean milk 25-35
Millet juice 10-20
Corn juice 10-20
Dusty yeast 1-2
Multitudinous sugared 0.5-3
Glucose 1-3
Peptone 0.5-1
Agar 0.8-1.5
KH
2PO
4 0.2-0.5
K
2HPO
4 0.05-1
Mnso
4 0.2-0.5
Caco
3 0.5-1
Znso
4 0.2-0.5
Mncl
2 0.1-0.3
Vitamin B1 0.3-1
Peanut oil 0.3-0.5
Water surplus
4. according to the preparation method of the said biologic compound amylose nutrient fluid with high efficiency of claim 3, it is characterized in that in the above step in the liquid culture medium layoutprocedure in fermentation or the breeding jar, with peeling potatoes and eye, section adds 6-9 times of weight water, boil to stir and be pasty state, corn flour adds 25-30 times of weight water boil, millet kept 30 minutes after adding 30-40 times of weight water boil, 3-6 jin soya bean is made 100 jin of soya-bean milk, above semi-finished product form said potato juice through getting filtrate with 6-8 layer filtered through gauze, corn flour juice, millet juice, soya-bean milk, accurately take by weighing required various medicine according to the above compatibility that provides then, the back adds agar and stirs to dissolving fully, carry out steam high-voltage sterilizing, be cooled to below 30 ℃ standby.
5. according to the preparation method of the said biologic compound amylose nutrient fluid with high efficiency of claim 1, it is characterized in that prepared composite nutrient-fluid is further made pulvis by spray-drying in the above steps d, make compound polysaccharide capsule or electuary or tablet again.
6. according to the preparation method of the said biologic compound amylose nutrient fluid with high efficiency of claim 1, it is characterized in that prepared composite nutrient-fluid is that the employing milipore filter separates concentrated or the reverse osmosis membrane purifying concentrates and the complex polysaccharide nutrient solution of acquisition in the above step.
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