CN101709308A - Method for producing fungus powder and fungus polysaccharide by using wheat alcohol waste lees solution - Google Patents
Method for producing fungus powder and fungus polysaccharide by using wheat alcohol waste lees solution Download PDFInfo
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- CN101709308A CN101709308A CN200910066217A CN200910066217A CN101709308A CN 101709308 A CN101709308 A CN 101709308A CN 200910066217 A CN200910066217 A CN 200910066217A CN 200910066217 A CN200910066217 A CN 200910066217A CN 101709308 A CN101709308 A CN 101709308A
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Abstract
The invention discloses a method for producing fungus powder and fungus polysaccharide by using a wheat alcohol waste lees solution, which relates to a technique for recycling the wheat alcohol waste lees solution and comprises the following steps: a, obtaining a clear solution; b, obtaining a strain propagation culture medium to perform strain propagation; c, culturing a seed in a fermentation tank; d, adding a fermentation broth containing mycelia into a filter cake obtained in the process of extracting polysaccharide so as to adjust the water content and obtain the edible and medicinal fungus powder; and e, producing edible and medicinal fungus polysaccharide, namely obtaining an edible and medicinal fungus polysaccharide product according to the step c. The method fulfills the aims of comprehensively utilizing the wheat alcohol waste lees solution and changing the waste into valuable, and can convert the waste lees solution into a high-yield raw material for health foods and precious medicaments.
Description
[technical field]
The invention belongs to the reutilization technology of a grow wheat alcohol waste lees solution, especially relate to a kind ofly utilize that the production of wheat alcohol waste lees solution is edible, the method for medicinal fungi bacterium powder and fungus polysaccharide.
[background technology]
Known, zymamsis adopts mainly that corn, potato are done, waste molasses etc. is raw material, because the development of aquaculture, certainly will cause the anxiety of raw material; The region price difference can appear in some area, and source of goods is instability etc. relatively also.Particularly corn price goes up in recent years, makes existing grain distillery present the loss situation, and with the restriction in the place of production, some producers just select for use wheat as the raw material of producing alcohol for above-mentioned reasons.Extensively be distributed in the wide geographic area to the north of the Changjiang river in the main producing region of China's wheat, so the supply of wheat is also comparatively well-to-do, a lot of alcohol producers are the main raw material of producing alcohol with the wheat, generally possess certain superiority at alcohol industry, moreover wheat possess following comparative benefits:
1, to have mouthfeel good, little sweet for the alcohol of being produced as raw material with wheat, as the raw material of blended liquor the very big market competitive power arranged;
2, produce intermediates such as gluten powder with wheat in process of production as raw material, the world market of gluten powder is very big in recent years, can obviously improve value-added content of product, and economic benefit also is improved relatively;
3, the DDG feed protein of being produced as the raw material of making alcohol with wheat is 26% " approximately ", and fat is 3% " approximately ", and color and luster is good, and it is edible that animal is comparatively liked, and welcome by the raiser.
At present, a lot of Alcohol Production manufacturers are raw material production alcohol with the wheat, from present trend, and the possibility that makes further progress.
Temperature during granulation is up to more than 85 ℃, and temperature is also higher, so will reduce moisture content after the granulation, its content is 7-12%.Can serious fouling after vaporizer operation for some time, so will shut down cleaning after a while, fouling is that protein precipitation deposits in heating tube, washes so be heated to 90～95 ℃ with 1～3% soda lye usually.
Known is the waste dregs liquid that raw material production alcohol is produced with wheat, owing to contain a large amount of Mierocrystalline celluloses and hemicellulose, solvend is more in the poor water of alcohol, has certain utility value; But the wheat waste dregs liquid is with respect to other raw material, and the concentration of heavy syrup is higher, and viscosity is bigger, and difficulty is cleaned in the easy fouling of vaporizer; So the backflow Water reuse also is subjected to certain restriction, the organism in the long-time recirculation water, fiber substance accumulation will be unfavorable for the zymic growth, and shorten the cycle of recycling water utilization, and prior art is difficult to again solve.
For these reasons as can be known, the processing of wheat alcohol waste lees solution, being one has technical barrier to be solved.This moment, we associated, edible, the most kinds of medicinal fungi, and they can decompose absorption Mierocrystalline cellulose and hemicellulose.
The applicant is in the patent of first to file " patent name, the method for producing edible, medicinal fungi bacterium powder and fungus polysaccharide; The applying date, on December 8th, 2000; Granted publication number, CN1236048C " and since demand at that time with the corn alcohol waste dregs liquid be that raw material production is edible, medicinal fungi bacterium powder and fungus polysaccharide; though achieve success; come compared with the wheat alcohol waste lees solution, the corn alcohol waste dregs liquid is then limited owing to raw material, so the corresponding reduction of using value; Major cause has two, 1, contain in the wheat alcohol waste lees solution and enrich Mierocrystalline cellulose and hemicellulose, is the rare microbe groups that can utilize xylogen, Mierocrystalline cellulose, hemicellulose for edible, medicinal fungi; 2, the applicant also finds in addition, because wheat alcohol waste lees solution organic substance than corn alcohol waste dregs liquid content height, is more suitable in edible, the submerged fermentation of medicinal fungi class.
[summary of the invention]
In order to realize described goal of the invention, the invention discloses a grow wheat alcohol waste lees solution and produce the method for fungi bacterium powder and fungus polysaccharide, the method of the invention is difficult at the wheat alcohol waste lees solution, occur environmental protection easily and exceed standard, and edible, medicinal fungi can decompose the situation of utilizing reluctant Mierocrystalline cellulose in the wheat alcohol waste lees solution, hemicellulose and high-enriched organics matter; The present invention is in conjunction with wheat alcohol waste lees solution rich cellulose and hemicellulose, the characteristics that dissolved organic matter content is high, with its eat, the production of medicinal fungi bacterium powder and fungus polysaccharide; The present invention is edible by utilizing, medicinal fungi decomposes, absorb Mierocrystalline cellulose, hemicellulose and other organism in the waste dregs liquid, solve behind the wheat processing alcohol usually waste dregs liquid as waste discharge, cause the problem of the wasting of resources of environmental pollution, realized the comprehensive utilization of wheat alcohol waste lees solution, the purpose that turns waste into wealth, and waste dregs liquid can be changed into the protective foods of Peak output and valuable pharmaceutical raw material.
The present invention realizes by following chemical synthesising technology scheme:
One grow wheat alcohol waste lees solution is produced the method for fungi bacterium powder and fungus polysaccharide, and described wheat alcohol waste lees solution is raw material with the wheat, through liquid submerged fermentation, brews alcohol, and obtains alcohol waste lees solution, comprises the steps:
A, acquisition clear liquid:
Solid in the described wheat alcohol waste lees solution is separated by liquid separation, obtained clear liquid;
B, obtain the bacterial classification substratum that spreads cultivation, carry out bacterial classification and spread cultivation;
1), slant strains medium preparation and slant strains spread cultivation; The slant strains substratum adopts the PDA synthetic medium to be inoculated in 24-27 ℃ and cultivated 10-15 days down.
2), the shake-flask culture base, the medium preparation and the seed of seed fermentation jar spread cultivation:
The shake-flask culture base is identical with seed fermentation jar substratum, and promptly step clear liquid that a obtains adds the 0-0.5% yeast extract paste; 0-4% sucrose transfers pH value to 5.5-6.5 with NaOH and HCl;
Shake-flask culture: get in the aforementioned triangular flask that obtains 250 milliliters of things, the substratum of the 70-90 milliliter of packing into, 750 milliliters of triangular flasks, loading amount is 100-120 milliliter/bottle; After the sterilization, insert slant strains when being cooled to 30 ℃ and " grow on the PDA substratum, be no more than the bacterial classification of 10 days edible, medicinal fungi " with next fritter of inoculation Shovel Shovel, approximately 1cm
2Left and right sides pure growth under 24-27 ℃ of condition, leaves standstill and was placed on the shaking table 160-180rpm in 24 hours in about 24-27 ℃, shaking culture 48-72 hour;
C, seed fermentation jar are cultivated:
In general form mechanical agitating fermentation tank or air lift type gas stirring formula fermentor tank, carry out; The 5-10% inoculum size is pressed in conventional sterilization back, inserts shake-flask seed, and its fermentation condition is: adopt the mechanical agitation type fermentor tank; 0-12 hour: stirring velocity was 80-100rpm, and air flow is 0.5: 1vvm; Or 12-72 hour stirring velocity 120rpm-140rpm air flow is 0.5: 1vvm, adopt air lift type pneumatic blending formula fermentor tank; Or 0-12 hour the time 0.25: 1vvm, 12-72 hour is 0.5: 1vvm; Wherein seed fermentation jar culture temperature is 24-27 ℃; Fermentation period is 48-72 hour;
After obtaining fermention medium, eat, the medicinal fungi liquid submerged fermentation: the composition of fermention medium with shake bottle, the substratum that spreads cultivation of seed fermentation jar is identical; Be that step clear liquid that a obtains adds the 0-0.15% yeast extract paste, 0-4% sucrose transfers pH value to 5.5-6.5 with NaOH and HCl; Conventional then sterilization, the inoculum size that 5-10% is pressed in the sterilization back inserts seed;
Wherein fermentation control: preceding 72 hours identical with seed fermentation jar condition, uses mechanical agitating fermentation tank; 72-144 hour, stirring velocity was 160-200rpm, and ventilation is 0.5: 1vvm, the condition of airlift bioreactor pneumatic blending; 72-144 hour is 0.75: 1vvm; Leavening temperature is 24-27 ℃, enters production edible, medicinal fungi bacterium powder then, wherein ferments to about 144 hours by this step, and reducing sugar descends and slows down, and can stop fermentation;
D, the Lu cake that obtains when fermented liquid " is included mycelium " and add to extract polysaccharide are to reconcile water content; By colloidal mill, grinding, homogeneous, make its matrix granule less than 5 microns, by centrifugal spray dryer, be spray dried to edible, medicinal fungi bacterium powder, wherein moisture content is≤10%;
The production of e, edible, medicinal fungi polysaccharide: c set by step, when fermentation stops temperature in the fermentor tank is controlled to 90 ℃, kept the Plate Filtration that about 35 ℃, carries out to be cooled 5 hours; Collect filtrate then, vacuum concentration is to about 1/5 of original volume; By centrifugal spray dryer, spraying drying becomes edible, medicinal fungi polysaccharide product; Described filter cake, stand-by when producing the bacterium powder.
Described wheat alcohol waste lees solution is produced the method for fungi bacterium powder and fungus polysaccharide, and described PDA synthetic medium is potato 200 grams, sucrose 20 grams, potassium primary phosphate 3 grams, sal epsom 1.5 grams, 1000 milliliters in water, agar 20 grams.
Described wheat alcohol waste lees solution is produced the method for fungi bacterium powder and fungus polysaccharide, being processed as of described PDA synthetic medium: 200 gram potatos, clean peeling is cut into small pieces, add water 1000ml and boiled half hour or steaming and decocting under high pressure 20 minutes, filtered through gauze, add sucrose 20 grams more successively, potassium primary phosphate 3 grams, sal epsom 1.5 gram, 1000 milliliters in water and agar 20 grams, fully filtered through gauze while hot after the dissolving, the packing test tube, the about 5-10ml of every test tube " decides on the test tube size ", and test tube pendulum inclined-plane is taken out, cooling back stored for future use in about 20 minutes backs of 15 pounds of steams " 121 ℃ " sterilization.
Described wheat alcohol waste lees solution is produced the method for fungi bacterium powder and fungus polysaccharide, the acquisition clear liquid process of described step a is: utilize whizzer that described wheat alcohol waste lees solution centrifugation is obtained described clear liquid, or utilize filter type to obtain described clear liquid, or after the clear liquid that described centrifugal or filter type obtains concentrated once more thin up obtain described clear liquid.
Described wheat alcohol waste lees solution is produced the method for fungi bacterium powder and fungus polysaccharide, with the substratum and the fermention medium of step b or c acquisition shake-flask culture base seed fermentation jar; Shaking the clear liquid that the substratum of bottle, seeding tank, fermentor tank all obtained by step a with the wheat alcohol waste lees solution is that parent adds yeast extract paste 0-0.5%, and sucrose 0-4% transfers pH value 5.5-6.5 and makes.
Described wheat alcohol waste lees solution is produced the method for fungi bacterium powder and fungus polysaccharide, steps d, after fermentation stops, add the filter cake that obtains when extracting polysaccharide and transfer water content, earlier with colloidal mill, grind to form＜5 microns matrix granules, without Plate Filtration, directly use the centrifugal spray dryer spraying drying, make edible, medicinal fungi bacterium powder.
Described wheat alcohol waste lees solution is produced the method for fungi bacterium powder and fungus polysaccharide, and described step e is when fermentation stops, in fermentor tank, fermented liquid directly is warming up to 90 ℃, keeps 5 hours, to be cooled to about 35 ℃, carry out Plate Filtration, collect filtrate, vacuum concentration is to 1/5 of original volume, by the centrifugal spray dryer spraying drying, wherein condition is that inlet temperature transfers to 200-230 ℃, and air outlet temperature transfers to 90 ℃, and spouting liquid is 500-1000kg/h; Make edible, medicinal fungi polysaccharide product; Use when the filter cake that obtains of Plate Filtration wherein, bacterium powder to be produced.
According to fungi bacterium powder or the fungus polysaccharide that above-mentioned processing step obtains, can be used as medicinal, edible dual-purpose fungi, except being the protective foods of high protein and low fat, they also have specific drug effect.Because the development that medicinal fungi is produced, driven the synchronized development of pharmaceutical industry, foodstuffs industry, have new industrial chain initiating terminal effect, the medicinal fungi that the present invention obtains can be used as the raw material of pharmaceutical factory and source mill; With medicinal fungi is that the fungi-medicine that raw material is made also will be greatly developed;
Following table is for the medicinal fungi being the fungi-medicine that raw material is made:
| White fungus and honey mushroom | Sanming City fungal studies institute | The sweet sheet of silver | Coronary heart disease, stenocardia |
| Cordyceps sinensis (Cordyceps sinensis) | Jiangxi Traditional Chinese Medicine Factory of medicine institute of medical courses in general institute | Paecilomyces hepiall chen | Hyperlipidemia, sexual disorder, chronic bronchitis |
| Chinese scholartree ear (Trametes robiniophila) | Jiangsu Qidong City pharmaceutical factory of Nanjing University of Traditional Chinese Medicine | Chinese scholartree ear electuary | Set upright to press down and loose, be applicable to former generation liver |
Need to prove, along with the pharmaceutical use of medicinal fungi is confirmed that gradually demand increases year by year by medical treatment is clinical.Growth needs xylogen, Mierocrystalline cellulose, the hemicellulose of most medicinal fungis so the demand of forest is also risen year by year, in the area of some centralized production, have caused people's attention to the breaking-up of the forest reserves.So change single cultivation basswood in the past, the method that saw is not cultivated is also imperative.
According to the analysis and the pharmacology clinical observation of Chemical Composition, the mycelium of some medicinal fungi and sporophore have same medical treatment and health-care effect.Over 40 years, along with the rise and the development of fermentation industry, utilizing liquid submerged fermentation to produce officinal fungus mycelium pharmacy route has had very great development.The mycelial fermentation culture cycle is short, is suitable for large-scale industrialized production.Cordyceps sinensis, Hericium erinaceus (Bull. Ex Fr.) Pers. filament also all have manufacturer's scale operation.The Hericium erinaceus (Bull. Ex Fr.) Pers. filament only throughput in Shanghai City every year can reach more than 400 ton." JINSHUIBAO " capsule that utilizes the Cordyceps mycelium submerged fermentation to produce becomes first Chinese medicine that goes through to produce one kind new medicine of China; This shows that market is big.
Fungi bacterium powder or fungus polysaccharide advantage that wheat alcohol waste lees solution of the present invention is produced are:
1, turns waste into wealth: wheat alcohol waste lees solution rich cellulose, hemicellulose and other organic substance, though it belongs to refuse as brewing alcohol, but, medicinal fungi edible for majority, it then is rare natural medium, allotment a little just can become some edible, medicinal fungi optimal medium.
2, with low cost: wheat alcohol waste lees solution (Vinasse) is the byproduct of fermentative Production alcohol, is a kind of organic waste water of high density, produces edible, medicinal fungi bacterium powder and fungus polysaccharide with it, greatly reduces cost, has improved the market competitiveness.
3, improve the quality of products: the wheat alcohol waste lees solution is not or seldom solid substance arranged, and traditional production substratum contains a large amount of solid substances, the product that the former produced contains the impurity except that mycelium and meta-bolites hardly, and the latter is then contained the impurity more than 2/3.Therefore, better with bacterium powder and polysaccharide product quality that the wheat alcohol waste lees solution is produced, its health care and efficacy component content also are better than traditional product accordingly.
Technology of the present invention has following characteristics:
1, environmental protection, discharge seldom; Whole production technology does not have or very a spot of discharge substantially, has avoided the pollution of environment, and the course of processing that makes Alcohol Production become green, safety becomes possibility.
2, product effect of fine quality is high; In traditional bacterium powder production process, fermentation stops the back by Plate Filtration, collection filter cake, drying, pulverizing, and filtered solution discards, but the extracellular products in the fermented liquid much is health care, efficacy component, and what abandon is huge waste.Technology of the present invention then is after fermentation stops, by the even matter of colloidal mill, grind to form 5 microns matrix granule, the broken wall process that in this process, has then comprised fungi, make in the product and keep healthy, efficacy component increases considerably, so the product that the present invention obtains is preferably, and overcomes described product defects.
Production technique edible, the medicinal fungi polysaccharide of the present invention also has following characteristics
The method that wheat alcohol waste lees solution of the present invention is produced fungi bacterium powder and fungus polysaccharide has realized following effect;
1. environmental protection, zero release.The traditional technology of fungus polysaccharide is to extract fungus polysaccharide to adopt filtering fermentation liquor, concentrates, and steps such as alcohol is analysed, drying, pulverizing realize.And our technology, fermentation stops, and handles 5 hours for 90 ℃, Plate Filtration is collected filtrate, Er Lv Ping is used to align the fermented liquid water content when making the bacterium powder.Then by concentrating, spraying drying is produced polysaccharide to filtrate, does not have discharge substantially and influences surrounding environment.
2. product high-quality, efficient.Existing polysaccharide product of analysing through alcohol because existing production technique science, unreasonable not runs off its nourishing function that has or efficacy component in a large number, though so fungus polysaccharide purity higher, curative effect is not comprehensive.And the production technique of fungus polysaccharide of the present invention had both been considered the extraction (keeping 5 hours for 90 ℃) of polysaccharide, considered the loss (remove heating, outside water is proposed, need not alcohol analyse) of efficacy component again, and polysaccharide content can reach more than 30%, and better curative effect is arranged.
3. the active substance that yeast fermentation produces is rich in the present invention.The wheat alcohol waste lees solution contains 20 seed amino acids, 10 several vitamins and several kinds of mineral elements, and these health care and treatment of diseases for people will have great help.
Owing to adopted technique scheme, the present invention to have following beneficial effect;
Wheat alcohol waste lees solution of the present invention is produced the method for fungi bacterium powder and fungus polysaccharide, utilizes that the production of wheat alcohol waste lees solution is edible, medicinal fungi bacterium powder and its advantage of fungus polysaccharide product be as follows:
1. a kind of new way of utilizing the wheat alcohol waste lees solution to produce high value added product is provided.
2. with the wheat alcohol waste lees solution---the organic waste water of this high density utilizes as a kind of resource again, and manufacturer reaches zero release, has solved the pollution problem of wheat zymamsis.
3. make the quality product of edible, medicinal fungi bacterium powder and fungus polysaccharide that further raising has been arranged.
4. the cost of edible, medicinal fungi bacterium powder and fungus polysaccharide is significantly reduced.
5. use method of the present invention, utilize that the production of wheat alcohol waste lees solution is edible, medicinal fungi bacterium powder and fungus polysaccharide will obtain huge economic benefit and obvious social.
[embodiment]
With reference to the following examples, can explain the present invention in more detail; But should be noted that the present invention is not limited to following embodiment.
Wheat alcohol waste lees solution of the present invention is produced the method for fungi bacterium powder and fungus polysaccharide, the technology of the method for, medicinal fungi bacterium powder edible with the production of wheat alcohol waste lees solution and fungus polysaccharide; Comprising: obtain alcohol waste lees solution after the zymamsis; Utilize alcohol waste lees solution to be reduced into clear liquid through thin up by centrifugal, filtration or clear liquid concentrated solution.
Also comprise, utilize throw out to make all-round dry feed.
Also comprise the substratum that utilize the clear liquid of waste dregs liquid to blend, to allocate the shake-flask culture base, seeding tank spreads cultivation and the fermention medium of fermentor tank.Promptly in clear liquid, add 0-0.5% yeast extract paste, 0-4% sucrose, transfer pH value to 5.5-6.5 with NaOH and HCl.
The bacterial classification that the present invention uses is matsutake (Trichotoma matsutake), derives from northeast food (medicine) fungal studies institute; Peacilomyce hepiahi (Paecilomyces hepiali) derives from Liaoning Province microbe research institute; The present invention also can use similar or identical but be matsutake, the peacilomyce hepiahi of other institute or manufacturer production.The method that bacterial classification is preserved is the physiological saline cryopreservation.
The method of strain expanded culture adopts the conventional method that spreads cultivation step by step, comprises the steps:
1. preparation slant medium: slant medium adopts the PDA substratum, according to a conventional method preparation.
2. obtaining liq substratum: comprise shake-flask culture base, the seeding tank substratum that spreads cultivation, two kinds of substratum are formed identical.
(1) clear liquid centrifugal with alcohol waste lees solution or that filtration obtains adds the yeast extract paste of 0-0.5%; 0-4% sucrose transfers pH value to 5.5-6.5 with NaOH and HCl.
(2) become 15-30 times of solution to original volume with the concentrated solution thin up of clear liquid, add the 0-0.5% yeast extract paste, 0-4% sucrose transfers pH value to 5.5-6.5 with NaOH and HCl.
3. the activation culture of slant strains: use Inoculant, the inoculation of agent piece was cultivated 10-15 days for 25-27 ℃ on the PDA substratum.
4. shaking bottle spreads cultivation: get 250 milliliters of triangular flasks and steam into 70-90 ml shake flask substratum, after the conventional sterilization, insert slant strains 1cm when being cooled to 30 ℃
2/ bottle.Prior to leaving standstill after 24 hours 160-180rpm on shaking table under 25-27 ℃, 24-27 ℃ following shaking culture 48-72 hour.
5. seeding tank spreads cultivation: seeding tank spreads cultivation after the conventional tinning of substratum, the sterilization, when being cooled to 25 ℃, press the inoculum size of 5-10%, the access shake-flask seed.Before inoculation, can be in sterilisable chamber by inoculum size, strict aseptic technique is with many bottles and become the inoculation of big triangular flask.
Universal machine stirred fermentor 0-12 hour, stirring velocity are 80-100rpm, and ventilation is 0.5: 1vvm, 12-72 hour stirring velocity 120-140rpm, air flow are 0.5: 1vvm.
Airlift bioreactor, the condition of aeration-agitation are 0-12 hour, and 0.25: 1vvm, air flow was 0.5 in 12-72 hour: 1vvm.Culture temperature is 24-27 ℃, approximately cultivates 48-72 hour.
After this, edible, medicinal fungi bacterium powder of fermention medium preparation and fermentative production and fungus polysaccharide.
The preparation of fermention medium with shake the bottle identical with seed fermentation jar substratum.After conventional tinning, the sterilization, the inoculum size of pressing 5-10% inserts the seed of seeding tank, and the control and the seeding tank of 0-72 hour air flow are identical.72-144 hour, general stirred-tank fermenter, stirring velocity is that the 160-200rpm ventilation is 0.5: 1vvm.Airlift fermentor 0.75: 1vvm.Leavening temperature is controlled at 24-27 ℃.
Produce method edible, medicinal fungi bacterium powder:
After the abovementioned steps, treat that fermentation stops, fermented liquid (comprising mycelium) adds the filter cake that fungus polysaccharide production obtains, with the levelling water content.Make its largest particle be no more than 5 microns by colloidal mill grinding, homogeneous, use the centrifugal spray dryer spraying drying, spray-dired processing condition are 230 ℃ of inlet temperature, 90 ℃ of air outlet temperatures; Spray amount is 1000kg/h, and product volume 300kg/h is packaged into edible, the medicinal fungi bacterium powder of product at last.
Produce method edible, the medicinal fungi polysaccharide:
Perhaps, treat that fermentation stops, and rises to 90 ℃ with the fermented liquid temperature, and keeps 5 hours.Be cooled to 35 ℃, filter by flame filter press.Collect filter cake, join the usefulness of reconciling water content in the fermented liquid during fungi bacterium powder to be produced.
Then, collect filtered solution, by the centrifugal spray dryer spraying drying, spray-dired processing condition are 230 ℃ of inlet temperature, 90 ℃ of air outlet temperatures to be condensed into 1/5 (5 times concentrate); Spray amount is 1000kg/h, and product volume 300kg/h is packaged into product at last; Edible, medicinal fungi polysaccharide.
Embodiment 1: with wheat alcohol waste lees solution production Phellinus bacterium powder and Phellinus polysaccharide;
One, the preparation of wheat alcohol waste lees solution clear liquid:
Collect fresh wheat alcohol waste lees, utilize flame filter press, filtering method is collected filtrate; Be the clear liquid of wheat alcohol waste lees solution.
Two, the processing condition of Phellinus liquid submerged fermentation and zymotechnique
1. the processing condition of Phellinus fermentation
(1) slant strains spreads cultivation:
The preservation bacterial classification of Phellinus (Phellinus igniarius) is available from northeast food (medicine) fungal studies institute.
Slant medium: PDA synthetic medium (potato 200 grams, sucrose 20 grams, potassium primary phosphate 3 grams, sal epsom 1.5 grams; agar 20 grams, 1000 milliliters in water) with 25 * 250mm test tube, preserve bacterial classification inoculation with Phellinus; cultivated 10 days, and under 0-4 ℃ of condition, preserved standby for 27 ℃.
(2) shaking a bottle bacterial classification spreads cultivation
The preparation of shake-flask culture base:
The shake-flask culture base is the wheat alcohol waste lees solution, filters through flame filter press, obtains clear liquid, adds 0.25% yeast extract paste in clear liquid, and 1.5% sucrose is transferred pH value to 6.0 with NaOH; 250 milliliters of triangular flask loading amounts are 70 milliliters/bottle.And 750 milliliters of triangular flask loading amounts are the 100-120 milliliter.Conventional sterilization is cooled to 30 ℃, inserts slant strains, after 24 hours, places 160-180rpm on the bottle swingging machine prior to 27 ℃ of static cultivations, 27 ℃ of shaking culture 72 hours.
(3) seeding tank spreads cultivation
The substratum of seeding tank is identical with the shake-flask culture base, after the conventional tinning sterilization, is cooled to 27 ℃, and the inoculum size by 10% inserts shake-flask seed.
Adopt the general form mechanical agitating fermentation tank.0-12 hour revolution is 80-100rpm, and air flow is 0.5: 1vvm; 12-72 hour stirring revolution is 120-140rpm, and air flow is 0.5: 1vvm, culture temperature is 27 ℃.
(4) ferment tank
Fermention medium is identical with the shake-flask culture base with the seed tank culture base.
Adopt the mechanical agitation type fermentor tank, after sky disappears, substratum is squeezed into fermentor tank, advancing tank volume is 70%.After the conventional sterilization, be cooled to 27 ℃,, insert the seed of seeding tank by 10% inoculum size.The same seeding tank of condition before the control of fermentor tank 72 hours, in the time of 72-144 hour, stirring velocity is 160-200rpm, air flow is 0.5: 1vvm, leavening temperature are controlled at 27 ℃.
2. the zymotechnique route of Phellinus
Three, the production of Phellinus bacterium powder
After treating that fermentation stops, Phellinus filter cake or Phellinus filter cake powder with fermented liquid and the acquisition of extraction polysaccharide add in proportion, its objective is and reconcile Phellinus fermented liquid water content, to reach 70-75%.Adopt colloidal mill, grinding, homogeneous make its matrix granule less than 5 microns.Adopt centrifugal spray dryer to carry out spraying drying, its processing condition are 230 ℃ of inlet temperature, 90 ℃ of air outlet temperatures, and spray amount is 500-1000kg/h, product volume is 100-300kg/h.Be packaged into product at last, i.e. Phellinus bacterium powder.
Four, the production of Phellinus fungus polysaccharide
Treat that fermentation stops back (fermenting process is produced with the bacterium powder), fermented liquid is heated to 90 ℃, keeps 5 hours.To be cooled during to 35 ℃, Plate Filtration is used during filter cake bacterium powder to be produced.Collect filtered solution, vacuum concentration to 1/5 original volume.With centrifugal spray dryer spraying drying (its condition is identical with the spraying drying condition of bacterium powder), packing becomes product, i.e. Phellinus fungus polysaccharide at last.
Phellinus of the present invention is a kind of medicinal fungi, and its polysaccharide and bacterium powder product have special efficacy to the treatment cancer.Also has a lot of nourishing functions simultaneously.
Part not in the detailed description of the invention is a prior art.
The embodiment that selects for use in this article in order to disclose purpose of the present invention currently thinks to suit, but will be appreciated that, the present invention is intended to comprise that all belong to all changes and the improvement of the interior embodiment of this design and the scope of the invention.
Embodiment 2: produce the Tricholoma matsutake (lto et lmai) Singer powder with the wheat alcohol waste lees solution
One, the preparation of wheat alcohol waste lees solution clear liquid
Collect the concentrated solution of fresh wheat alcohol waste lees solution clear liquid, with 20 times of clear liquids (clear liquid reduced liquid) that are the wheat alcohol waste lees solution of its dilution.
Two, the processing condition and the zymotechnique of matsutake fermentation
1. the processing condition of matsutake fermentation
(1) slant culture bacterial strain: matsutake (Trichotoma matsutake) is preserved bacterial classification.Available from northeast food (medicine) fungal studies institute.Substratum: the inoculation of PDA synthetic medium 25 * 250mm test tube, to produce bacterial classification and cultivated about 10 days for 24-27 ℃, 0-4 ℃ of preservation is standby.
(2) shake-flask culture: substratum is the concentrated solution that wheat alcohol waste lees solution clear liquid rapid steamer obtains, with 20 times of its dilutions, be reduced to clear liquid, the yeast extract paste of adding 0.25%, 1.5% sucrose adds NaOH and transfers PH to 6.0,250 milliliters of triangular flasks, 70 milliliters of loading amounts, and 750 milliliters of triangular flask loading amounts are the 100-120 milliliter.Conventional sterilization is cooled to 27 ℃, inserts slant strains, leaves standstill prior to 24-27 ℃ and is placed on 160-180rpm on the bottle swingging machine in 24 hours, 27 ℃ of constant temperature culture 72 hours
(3) seed tank culture: substratum after conventional tinning and the sterilization, is cooled to 27 ℃ with shaking bottle, and the inoculum size by 5% inserts shake-flask seed.Adopt the general form mechanical agitating fermentation tank, 0-12 hour revolution is 80-100rpm, and air flow is 0.5: 1vvm; Stirring velocity was 0.5 for the 120-140rpm air flow in 12-72 hour: the 1vvm culture temperature is 27 ℃.
(4) ferment tank: substratum and seed fermentation jar and shake-flask culture base are identical.Adopt the universal machine stirred fermentor, after ullage to be fermented disappears substratum is squeezed in the fermentor tank, advancing tank volume is 70%.After the conventional sterilization, be cooled to 27 ℃, the inoculum size by 10% inserts the bacterial classification in the seeding tank.Fermentating controling condition is identical with seeding tank before 72 hours, and 72-144 hour stirring velocity is 160-200rpm, and air flow is 0.5: 1vvm, leavening temperature are controlled at 27 ℃.
2. the zymotechnique route of matsutake
Three, the production of Tricholoma matsutake (lto et lmai) Singer powder
After treating that fermentation stops, extract matsutake filter cake or the filter cake powder that blazei polysaccharide obtains with adding in the fermented liquid.Adjust the fermented liquid water content to 70-75%, adopt colloidal mill grinding, homogeneous to make its matrix granule less than 5 microns.Adopt centrifugal spray dryer to carry out spraying drying, its processing condition are: 230 ℃ of inlet temperature, and 90 ℃ of air outlet temperatures, spray amount is 1000kg/h, product volume is 300kg/h.At last, packing becomes product, i.e. Tricholoma matsutake (lto et lmai) Singer powder.
Annotate: matsutake is a kind of rare edible fungus, and its sporophore can not artificial culture, and mycelium can be produced by the liquid submerged fermentation method.
Embodiment 3: produce Cordyceps polysaccharide with the wheat alcohol waste lees solution
One, the preparation of wheat alcohol waste lees solution clear liquid
Collect fresh wheat alcohol waste lees solution, obtain clear liquid by centrifugal method.
Two, the technological condition for fermentation of Cordyceps sinensis and zymotechnique
1. the technological condition for fermentation of Cordyceps sinensis
(1) bacterial strain.Peacilomyce hepiahi (Paecilomyces hepiali) derives from Liaoning Province microbe research institute.Seed is preserved in the inoculation of substratum PDA synthetic medium 25 * 250mm test tube.Cultivated 8-10 days for 24 ℃, 0-4 ℃ of preservation is standby.
(2) shake-flask culture, substratum are to add 0.25% yeast extract paste in the wheat vinasse waste dregs liquid clear liquid, and 1.5% sucrose is transferred pH value to 6.0 with NaOH.250 milliliters of triangular flask loading amounts are 70 milliliters; The bottled 100-120 milliliter of 750 milliliters triangle.Conventional sterilization was cooled to 24 ℃ of shaking culture 2.5 days.
(3) seed tank culture, substratum is with the shake-flask culture base.Conventional sterilization, be cooled to 24 ℃, shake a bottle bacterial classification by the access of 5% inoculum size, use the universal machine stirred fermentor, 0-12 hour rotating speed is 80-100rpm, air flow is 0.5: 1vmm, 12-72 hour, mixing speed was 120-140rpm, and air flow is 0.5: 1vvm, culture temperature is 24 ℃, and culture cycle is 72 hours.
(4) ferment tank: substratum is identical with the seed tank culture base.Adopt the universal machine stirred fermentor, after ullage to be fermented disappears, substratum is squeezed in the fermentor tank, advancing tank volume is 70%.After the conventional sterilization, be cooled to 24 ℃ by 10% inoculum size, insert the bacterial classification in the seeding tank, fermentation control is identical with the fermentating controling condition of seeding tank.48-72 hour stirring velocity is 160-200rpm, and air flow is 0.5: 1vvm, leavening temperature are controlled at 24 ℃, cultivate 72 hours.
2. the zymotechnique route of Cordyceps sinensis
Three, the production of Cordyceps polysaccharide
Treat that fermentation stops, the fermented liquid temperature is risen to 90 ℃, kept 5 hours.Be cooled to 35 ℃, filter with flame filter press and collect filtered solution, vacuum concentration is to 1/5 of original volume.Use the centrifugal spray dryer spraying drying; Technology controlling and process: air inlet temperature is 230 ℃, and air outlet temperature is 90 ℃, and spray amount 1000kg/h, product volume are 300kg/h.Packing becomes product, i.e. Cordyceps polysaccharide at last.The Lu cake of collecting is treated to use when the bacterium powder is produced.
Annotate: peacilomyce hepiahi (Paecilomyces hepiali) is a kind of Cordyceps sinensis, it is a kind of medicinal fungi, the very difficult artificial culture of worm summer in winter worm becomes sporophore, but can obtain mycelium by liquid submerged fermentation, can produce bacterium powder and Cordyceps polysaccharide with it.
Claims (7)
1. a grow wheat alcohol waste lees solution is produced the method for fungi bacterium powder and fungus polysaccharide, and it is characterized in that: described wheat alcohol waste lees solution is raw material with the wheat, through liquid submerged fermentation, brews alcohol, and obtains alcohol waste lees solution, comprises the steps:
A, acquisition clear liquid:
Solid in the described wheat alcohol waste lees solution is separated by liquid separation, obtained clear liquid;
B, obtain the bacterial classification substratum that spreads cultivation, carry out bacterial classification and spread cultivation;
1), slant strains medium preparation and slant strains spread cultivation; The slant strains substratum adopts the PDA synthetic medium to be inoculated in 24-27 ℃ and cultivated 10-15 days down;
2), the shake-flask culture base, the medium preparation and the seed of seed fermentation jar spread cultivation:
The shake-flask culture base is identical with seed fermentation jar substratum, and promptly step clear liquid that a obtains adds the 0-0.5% yeast extract paste; 0-4% sucrose transfers pH value to 5.5-6.5 with NaOH and HCl;
Shake-flask culture: get in the aforementioned triangular flask that obtains 250 milliliters of things, the substratum of the 70-90 milliliter of packing into, 750 milliliters of triangular flasks, loading amount is 100-120 milliliter/bottle; After the sterilization, insert slant strains when being cooled to 30 ℃ and " grow on the PDA substratum, be no more than the bacterial classification of 10 days edible, medicinal fungi " with inoculation
Next fritter, approximately 1cm
2Left and right sides pure growth under 24-27 ℃ of condition, leaves standstill and was placed on the shaking table 160-180rpm in 24 hours in about 24-27 ℃, shaking culture 48-72 hour;
C, seed fermentation jar are cultivated:
In general form mechanical agitating fermentation tank or air lift type gas stirring formula fermentor tank, carry out; The 5-10% inoculum size is pressed in conventional sterilization back, inserts shake-flask seed, and its fermentation condition is: adopt the mechanical agitation type fermentor tank; 0-12 hour: stirring velocity was 80-100rpm, and air flow is 0.5: 1vvm; Or 12-72 hour stirring velocity 120rpm-140rpm air flow is 0.5: 1vvm, adopt air lift type pneumatic blending formula fermentor tank; Or 0-12 hour the time 0.25: 1vvm, 12-72 hour is 0.5: 1vvm; Wherein seed fermentation jar culture temperature is 24-27 ℃; Fermentation period is 48-72 hour;
After obtaining fermention medium, eat, the medicinal fungi liquid submerged fermentation: the composition of fermention medium with shake bottle, the substratum that spreads cultivation of seed fermentation jar is identical; Be that step clear liquid that a obtains adds the 0-0.15% yeast extract paste, 0-4% sucrose transfers pH value to 5.5-6.5 with NaOH and HCl; Conventional then sterilization, the inoculum size that 5-10% is pressed in the sterilization back inserts seed;
Wherein fermentation control: preceding 72 hours identical with seed fermentation jar condition, uses mechanical agitating fermentation tank; 72-144 hour, stirring velocity was 160-200rpm, and ventilation is 0.5: 1vvm, the condition of airlift bioreactor pneumatic blending; 72-144 hour is 0.75: 1vvm; Leavening temperature is 24-27 ℃, enters production edible, medicinal fungi bacterium powder then, wherein ferments to about 144 hours by this step, and reducing sugar descends and slows down, and can stop fermentation;
D, the Lu cake that obtains when fermented liquid " is included mycelium " and add to extract polysaccharide are to reconcile water content; By colloidal mill, grinding, homogeneous, make its matrix granule less than 5 microns, by centrifugal spray dryer, be spray dried to edible, medicinal fungi bacterium powder, wherein moisture content is≤10%;
The production of e, edible, medicinal fungi polysaccharide: c set by step, when fermentation stops temperature in the fermentor tank is controlled to 90 ℃, kept the Plate Filtration that about 35 ℃, carries out to be cooled 5 hours; Collect filtrate then, vacuum concentration is to about 1/5 of original volume; By centrifugal spray dryer, spraying drying becomes edible, medicinal fungi polysaccharide product; Described filter cake, stand-by when producing the bacterium powder.
2. wheat alcohol waste lees solution according to claim 1 is produced the method for fungi bacterium powder and fungus polysaccharide, it is characterized in that: described PDA synthetic medium is potato 200 grams, sucrose 20 grams, potassium primary phosphate 3 grams, sal epsom 1.5 grams, 1000 milliliters in water, agar 20 grams.
3. wheat alcohol waste lees solution according to claim 1 and 2 is produced the method for fungi bacterium powder and fungus polysaccharide, it is characterized in that: being processed as of described PDA synthetic medium: 200 gram potatos, clean peeling is cut into small pieces, add water 1000ml and boiled half hour or steaming and decocting under high pressure 20 minutes, filtered through gauze, add sucrose 20 grams more successively, potassium primary phosphate 3 grams, sal epsom 1.5 gram, 1000 milliliters in water and agar 20 grams, fully filtered through gauze while hot after the dissolving, the packing test tube, the about 5-10ml of every test tube " decides on the test tube size ", and test tube pendulum inclined-plane is taken out, cooling back stored for future use in about 20 minutes backs of 15 pounds of steams " 121 ℃ " sterilization.
4. wheat alcohol waste lees solution according to claim 1 is produced the method for fungi bacterium powder and fungus polysaccharide, it is characterized in that: the acquisition clear liquid process of described step a is: utilize whizzer that described wheat alcohol waste lees solution centrifugation is obtained described clear liquid, or utilize filter type to obtain described clear liquid, or after the clear liquid that described centrifugal or filter type obtains concentrated once more thin up obtain described clear liquid.
5. according to the method for claim 1 or 4 described wheat alcohol waste lees solutions production fungi bacterium powder and fungus polysaccharide, it is characterized in that: the substratum and the fermention medium of step b or c acquisition shake-flask culture base seed fermentation jar; Shaking the clear liquid that the substratum of bottle, seeding tank, fermentor tank all obtained by step a with the wheat alcohol waste lees solution is that parent adds yeast extract paste 0-0.5%, and sucrose 0-4% transfers pH value 5.5-6.5 and makes.
6. the method for producing fungi bacterium powder and fungus polysaccharide according to claim 1 or 4 described wheat alcohol waste lees solutions, it is characterized in that: steps d, after fermentation stops, add the filter cake that obtains when extracting polysaccharide and transfer water content, earlier with colloidal mill, grind to form＜5 microns matrix granules, without Plate Filtration, directly use the centrifugal spray dryer spraying drying, make edible, medicinal fungi bacterium powder.
7. the method for producing fungi bacterium powder and fungus polysaccharide according to claim 1 or 4 described wheat alcohol waste lees solutions, it is characterized in that: described step e, when fermentation stops, in fermentor tank, fermented liquid directly is warming up to 90 ℃, kept 5 hours, to be cooled to about 35 ℃, carry out Plate Filtration, collect filtrate, vacuum concentration is to 1/5 of original volume, by the centrifugal spray dryer spraying drying, wherein condition is that inlet temperature transfers to 200-230 ℃, and air outlet temperature transfers to 90 ℃, and spouting liquid is 500-1000kg/h; Make edible, medicinal fungi polysaccharide product; Use when the filter cake that obtains of Plate Filtration wherein, bacterium powder to be produced.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103119171A (en) * | 2010-07-01 | 2013-05-22 | 塞尔特可再生能源有限公司 | Process for the manufacture of butanol or acetone |
| CN105950694A (en) * | 2016-07-25 | 2016-09-21 | 滨州中裕食品有限公司 | Novel process for co-producing wheat polysaccharide and bioactive peptide through alcohol fermentation waste liquid |
-
2009
- 2009-10-22 CN CN200910066217A patent/CN101709308A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103119171A (en) * | 2010-07-01 | 2013-05-22 | 塞尔特可再生能源有限公司 | Process for the manufacture of butanol or acetone |
| CN103119171B (en) * | 2010-07-01 | 2016-01-20 | 塞尔特可再生能源有限公司 | Manufacture the technique of butanols or acetone |
| CN105950694A (en) * | 2016-07-25 | 2016-09-21 | 滨州中裕食品有限公司 | Novel process for co-producing wheat polysaccharide and bioactive peptide through alcohol fermentation waste liquid |
| CN105950694B (en) * | 2016-07-25 | 2020-01-21 | 滨州中裕食品有限公司 | Novel process for co-producing wheat polysaccharide and active peptide by using alcohol fermentation waste liquid |
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