CN102697944B - Traditional Chinese medicine composition for treating kidney deficiency and preparation method thereof - Google Patents
Traditional Chinese medicine composition for treating kidney deficiency and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine composition for treating kidney deficiency and a preparation method thereof. The traditional Chinese medicine composition comprises epimedium, common cnidium fruit, desertliving cistanche, glossy privet fruit, prepared rehmannia root, Chinese magnoliavine fruit, songaria cynomorium herb, morinda root, seeds of Chinese dodder, palmleaf raspberry fruit, malaytea scurfpea fruit, ginseng, mantis egg-case, hairy deerhorn, hippocampus and the like. The preparation method includes: crashing residues into powder with the particle diameter smaller than 10 micrometers after ethanol extraction of the hairy deerhorn and the hippocampus, preparing other medicinal materials into extracts or dry extract powders via water extraction or ethanol extraction, and adding pharmaceutically acceptable additives to prepare oral solutions, powders, granules, tablets, capsules, soft capsules, pills, dripping pills and other oral medicaments. Since the residues of the two precious medicinal materials of the hairy deerhorn and the hippocampus are crushed to be used as medicine after ethanol extraction, contents of active ingredients in the medicaments are increased remarkably. The extracts of other medicinal materials are dried by the aid of a vacuum belt drying method, so that production efficiency is improved obviously, and energy saving and consumption reducing are achieved.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, particularly a kind for the treatment of Chinese medicine composition of suffering from a deficiency of the kidney and preparation method thereof.
Background technology
The traditional Chinese medical science thinks that kidney is very important internal organs, and the address of the congenital foundation is arranged.The major physiological function of kidney is: main store essential substances, main water, main improving inspiration by invigorating kidney-QI.Because kidney is hidden congenital essence, main reproduction is the origin of human life, so claim that kidney is " the congenital foundation ".The kidney essense kidney qi, the kidney qi forked rubbing, the negative and positive of whole body internal organs can be subsidized, be promoted, coordinate to kidney yin and kidney yang, so kidney is otherwise known as " the five internal organs negative and positive are originally ".Suffer from a deficiency of the kidney and refer to kidney vital essence negative and positive deficiency.The kind of suffering from a deficiency of the kidney has a lot, and wherein modal is deficiency of the kidney yin and insufficiency of kidney-YANG.The symptom of insufficiency of kidney-YANG is the soreness of waist, cold extremities, fear of cold, even also has edema, is the symptom of " trembling with fear "; The symptom of deficiency of the kidney yin is " heat ", mainly contains the soreness of waist, scorching, night sweat, sweating due to debility, dizziness, tinnitus etc.Modern science proves, when the people suffers from a deficiency of the kidney, and no matter deficiency of the kidney yin or insufficiency of kidney-YANG, the capital causes the reduction of people's immunocompetence, has more evidence to show, suffers from a deficiency of the kidney when taking place, the immunocompetence of kidney reduces, and the microcirculqtory system of kidney also can be blocked, and causes the kidney network obstructed.
Modern study thinks that the traditional Chinese medical science " kidney " essence not only comprises the function of hypophysis-gonad axis, has also comprised the neuroendocrine function of hypothalamus-hypophysis.Insufficiency of kidney-YANG is hypothalamic-pituitary-adrenal-low and disorderly major reason of thymus s function.Insufficiency of kidney-YANG can cause organ weights such as hypophysis, adrenal gland to alleviate, and serum testosterone in the serum (T), hydrocortisone (F) content reduce.Simultaneously, because the neutral hormone-content of the blood of yang deficiency patient descends, the negative feedback of hypophysis is obviously weakened, the short gonad function of hypophysis cerebri strengthens, lutropin (LH), and follicule-stimulating hormone (FSH) (FSH) secretion increasing, LH in the blood, FSH content rises.A large amount of pharmacological evaluation show, the insufficiency of kidney-YANG rat model generally show as food-intake and amount of drinking water reduces, the penis prolongation of latency, body of gland weight such as testis, seminal vesicle, prostate, levator ani and weight coefficient reduce; The mice of deficiency of the kidney yin model then shows as phenomenons such as basal metabolism is vigorous, weight loss, fervescence.And sympathetic activation, trilute (T3) and the rising of thyroxine (T4) level can appear, the ratio of the rising of blood plasma cyclic adenosine monophosphate (cAMP) content, the decline of cyclic guanosine monophosphate (cGMP) content, cAMP and cGMP raises, cellular immune function is low, degradation under thymus and the index and spleen index.
Suffering from a deficiency of the kidney is the distinctive medical science disease of theory of Chinese medical science title, and it is treated based on Chinese patent medicine, cooperates the method for an amount of health preserving body-building simultaneously, and the Chinese patent medicine kind that treatment is in the market suffered from a deficiency of the kidney is representative with JINGUI SHENQI WAN and LIUWEI DIHUANG WAN mainly.The Chinese medicine composition that treatment of the present invention is suffered from a deficiency of the kidney is made up of 15 flavor medical materials such as Herba Epimedii, Fructus Cnidii, Herba Cistanches, Fructus Ligustri Lucidi, Radix Rehmanniae Preparata, Fructus Schisandrae Chinensis, Herba Cynomorii, Radix Morindae Officinalis, Semen Cuscutae, Fructus Rubi, Fructus Psoraleae, Radix Ginseng, Ootheca Mantidis, Cornu Cervi Pantotrichum, Hippocampus, has warming the kidney to activate YANG, the effect of the YIN nourishing of reinforcing the kidney, lassitude due to being mainly used in suffering from a deficiency of the kidney, aching pain in waist and back, the soreness of the shank knee joint is soft, and aversion to cold and cold limbs, nocturia such as increase at disease.
Modern study shows that it is closely related with body's immunity to suffer from a deficiency of the kidney, and suffering from a deficiency of the kidney directly to influence generation and the adjusting of immunocyte.Cornu Cervi Pantotrichum and Hippocampus two flavor animal medicinal materials, the effect of have invigorating kidney-YANG, mending marrow, two flavor medical materials all contain nutritional labelings such as rich in protein, aminoacid, and qualitative characteristics and the drug action of these nutrients and Cornu Cervi Pantotrichum, Hippocampus are closely related.The protein that has bibliographical information to extract from Cornu Cervi Pantotrichum and Hippocampus all can significantly improve the content of mononuclear-macrophage phagocytic function and serum IgG to normal mouse, hydrocortisone or caused by cyclophosphamide immunosuppressant mice, the dilution factor of the specific immunity antibody hemolysin content of mice and serum agglutinin also all there is at double increase, particularly body immune system is in inhibitory state or the effect when weak is better.As seen, the height of content Determination of Icariin and the protein content that extracts from Cornu Cervi Pantotrichum, Hippocampus will directly have influence on the curative effect of preparation of the present invention in the Chinese medicine composition that treatment of the present invention is suffered from a deficiency of the kidney.Therefore, it is very important to improve the active component of icariin in the preparation and two flavor animal drugs to the full extent.
The present invention is the improvement invention on " Chinese prescription of yang invigorating functions, preparation and preparation technology thereof " (number of patent application CN93101612.6) patent basis, the preparation technology of prior art be with Cornu Cervi Pantotrichum, Hippocampus two flavor animal drugs earlier with the extraction of 75% alcohol dipping, medicinal residues again with other medical material together with water extraction, be condensed into extractum; Because the Cornu Cervi Pantotrichum quality is hard, adopt the extraction process of prior art, active component is difficult to extract fully in the medical material, and the Cornu Cervi Pantotrichum after the extraction, Hippocampus medicinal residues go out of use, and fail to obtain reasonable use.By to " using the Chinese medicine preparation contain Cornu Cervi Pantotrichum, Hippocampus to inquire about in Chinese pharmacopoeia and national Chinese patent medicine preparation standard, the Ministry of Public Health ministry standard, after not finding that Cornu Cervi Pantotrichum, Hippocampus adopt alcohol extraction (or water extraction) to make extractum or dried cream powder in the existing herbal species, medicinal residues are ground into the technology that powder is used as medicine; By patent and literature search, do not see have Cornu Cervi Pantotrichum, Hippocampus alcohol extraction (or water extraction) made extractum or dried cream powder after, medicinal residues are ground into relevant patent and the bibliographical information that powder is used as medicine.
Summary of the invention
One of the object of the invention provides the Chinese medicine composition that a kind for the treatment of is suffered from a deficiency of the kidney.
Two of the object of the invention provides the preparation method of the Chinese medicine composition that a kind for the treatment of suffers from a deficiency of the kidney.
The Chinese medicine composition that treatment of the present invention is suffered from a deficiency of the kidney is made up of following ingredients: Herba Epimedii 800 ~ 1200g, Fructus Cnidii 800 ~ 1200g, Herba Cistanches 800 ~ 1200g, Fructus Ligustri Lucidi 700 ~ 900g, Radix Rehmanniae Preparata 650 ~ 850g, Fructus Schisandrae Chinensis 500 ~ 700g, Herba Cynomorii 400 ~ 600g, Radix Morindae Officinalis 400 ~ 600g, Semen Cuscutae 300 ~ 500g, Fructus Rubi 300 ~ 500g, Fructus Psoraleae 300 ~ 500g, Radix Ginseng 200 ~ 350g, Ootheca Mantidis 250 ~ 350g, Cornu Cervi Pantotrichum 20 ~ 30g, Hippocampus 20 ~ 30g and pharmaceutically acceptable additives 1000-5000g.Wherein, described Cornu Cervi Pantotrichum, Hippocampus are made extractum or dried cream powder after extracting, and the Cornu Cervi Pantotrichum of mean diameter 1 ~ 10 μ m, Hippocampus medicinal residues micropowder; Described Radix Ginseng extracts makes extractum or dried cream powder; Described Herba Cistanches, Radix Morindae Officinalis, Fructus Psoraleae, Fructus Schisandrae Chinensis extract makes extractum or dried cream powder; Described Herba Epimedii, Semen Cuscutae, Ootheca Mantidis, Fructus Cnidii, Herba Cynomorii, Fructus Ligustri Lucidi, Radix Rehmanniae Preparata, Fructus Rubi are extracted and make extractum or dried cream powder; Described pharmaceutically acceptable additives are one or more in starch, sucrose, lactose, dextrin, microcrystalline Cellulose, hypromellose, polyvidone, carboxymethyl starch sodium, magnesium stearate, Pulvis Talci, Polyethylene Glycol, micropowder silica gel, vegetable oil, glycerol, paraffin, the tween 80.
The preferred composition of the Chinese medicine composition that treatment of the present invention is suffered from a deficiency of the kidney consists of Herba Epimedii 1000g, Fructus Cnidii 1000g, Herba Cistanches 1000g, Fructus Ligustri Lucidi 800g, Radix Rehmanniae Preparata 750g, Fructus Schisandrae Chinensis 600g, Herba Cynomorii 500g, Radix Morindae Officinalis 500g, Semen Cuscutae 400g, Fructus Rubi 400g, Fructus Psoraleae 400g, Radix Ginseng 300g, Ootheca Mantidis 300g, Cornu Cervi Pantotrichum 25g, Hippocampus 25g and lactose, dextrin, starch, microcrystalline Cellulose, carboxymethyl starch sodium, silicon dioxide, Pulvis Talci, in the magnesium stearate one or more.
Because the adding of Cornu Cervi Pantotrichum, Hippocampus medicinal residues micropowder, the Chinese medicine composition that treatment of the present invention is suffered from a deficiency of the kidney is compared with the oral liquid that reinforces the kidney of prior art for preparing, and the activity of proteins component content has significantly improved 37% in the compositions; Simultaneously, the pharmacologically active of Chinese medicine composition of the present invention obviously improves, and can significantly regulate the physiological function of insufficiency of kidney-YANG and deficiency of the kidney yin animal pattern.
The preparation method of the Chinese medicine composition that treatment of the present invention is suffered from a deficiency of the kidney comprises the steps:
(1), Radix Ginseng extracting in water 2 times, add the water of 8 ~ 12 times of medical material amounts for the first time, extracted 2 hours; Add for the second time the water of 6 ~ 10 times of medical material amounts, extracted 2 hours; Merge extractive liquid,, filter, filtrate is 60 ~ 90 ℃ of temperature, and steam pressure 0.05 ~ 0.15MPa, concentrating under the condition of vacuum 0.02 ~ 0.09MPa and making relative density is 1.25 ~ 1.35(30 ℃) extractum or with extractum 50 ~ 75 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 10L/h, further vacuum belt type drying becomes dried cream under the strip transfer rate 25cm/min condition, pulverizes, cross 40 ~ 80 mesh sieves, it is standby to make dried cream powder;
(2), Cornu Cervi Pantotrichum, Hippocampus add 65 ~ 85% ethanol of 6 ~ 8 times of medical material amounts, dipping extracted 24 hours, filter, filtrate is 50 ~ 80 ℃ of temperature, steam pressure 0.04 ~ 0.12MPa, concentrating under the condition of vacuum 0.02 ~ 0.09MPa and making relative density is 1.10 ~ 1.15(30 ℃) extractum or with extractum 40 ~ 55 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, further vacuum belt type drying becomes dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, it is standby to make dried cream powder;
(3), step (2) is extracted Cornu Cervi Pantotrichum, the oven dry of posthippocampal medicinal residues, pulverize, cross 80 mesh sieves, obtain fine powder, fine powder is 10 ~ 25kg/h with the air-flowing type superfine communication technique in charging rate again, air pressure is to carry out micronizing under 0.5 ~ 0.8MPa condition, and the medicinal residues micropowder of making mean diameter 1 ~ 10 μ m is standby;
(4), Herba Cistanches, Radix Morindae Officinalis, Fructus Psoraleae and Fructus Schisandrae Chinensis are made in the rearmounted extraction vessel only, 65 ~ 85% alcohol dipping that add 2 ~ 4 times of medical material amounts were extracted 72 hours, collect lixiviating solution, filter, filtrate is 50 ~ 80 ℃ of temperature, steam pressure 0.04 ~ 0.12MPa, concentrating under the condition of vacuum 0.02 ~ 0.09MPa and making relative density is 1.25 ~ 1.35(30 ℃) extractum or with extractum 40 ~ 55 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, further vacuum belt type drying becomes dried cream under the strip transfer rate 25cm/min condition, pulverizes, cross 40 ~ 80 mesh sieves, it is standby to make dried cream powder;
(5), Herba Epimedii, Semen Cuscutae, Ootheca Mantidis, Fructus Cnidii, Herba Cynomorii, Fructus Ligustri Lucidi, Radix Rehmanniae Preparata, Fructus Rubi make in the rearmounted extraction vessel only, extracting in water 2 times adds the water of 6 ~ 8 times of medical material amounts for the first time, extracts 2 hours; Add for the second time the water of 4 ~ 6 times of medical material amounts, extracted 2 hours; Merge extractive liquid,, filter, filtrate is 60 ~ 90 ℃ of temperature, steam pressure 0.05 ~ 0.15MPa, being condensed into relative density under the condition of vacuum 0.02 ~ 0.09MPa is 1.12 ~ 1.16(85 ℃) thick paste, thick paste is put into settling tank, adds 95% ethanol of 3 times of thick paste amounts, stirs, left standstill 24 hours, filter, filtrate is at 50 ~ 90 ℃ of temperature, steam pressure 0.04 ~ 0.12MPa, reduce pressure under the condition of vacuum 0.02 ~ 0.09MPa, concentrating and making relative density is 1.25 ~ 1.35(30 ℃) extractum or with extractum at 50 ~ 75 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, further vacuum belt type drying becomes dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 40 ~ 80 mesh sieves, it is standby to make dried cream powder;
(6), extractum or the dried cream powder with step (1), (2), (3), (4), (5) mixes with the medicinal residues micropowder, add pharmaceutically acceptable additives, make oral liquid, granule, tablet, powder, capsule, soft capsule, pill, drop pill, namely get the Chinese medicine composition that the present invention's treatment is suffered from a deficiency of the kidney.
If not refer in particular to, the used Chinese medicine material of the present invention and pharmaceutically acceptable additives are the commercial goods.
Defective at prior art for preparing technology, the preparation method of the Chinese medicine composition that treatment of the present invention is suffered from a deficiency of the kidney is with Cornu Cervi Pantotrichum, Hippocampus is extracted and to make mode that extractum is used as medicine and change into extracting earlier and make extractum or dried cream powder, be ground into the medicinal residues micropowder of mean diameter 1 ~ 10 μ m after the medicinal residues oven dry, medicinal residues micropowder and extractum or dried cream powder together are used as medicine, be used as medicine being ground into powder after the medicinal residues oven dry, not only significantly improved Cornu Cervi Pantotrichum in the Chinese medicinal composition preparation of the present invention, the active component content of Hippocampus, improved the curative effect of preparation, also reached the purpose that takes full advantage of valuable medicinal, avoided the waste of resource, the Chinese medicine composition that treatment of the present invention is suffered from a deficiency of the kidney adopts concentrating under reduced pressure to be prepared into the extractum except making oral liquid, the granule of making, tablet, powder, capsule, soft capsule, pill and drop pill all are that extractum further is dried to dried cream powder, in the technology of preparation medicine dried cream powder, the present invention has adopted the vacuum belt type drying technology that extractum is carried out drying, utilize the dried granule of vacuum belt drier that to a certain degree crystallization effect is arranged, simultaneously from microstructure, granule interior has microcellular structure, the open structure of granule interior can guarantee that it has good instant capacity, promotes the class of product greatly; Whole dry run is in the environment of vacuum, sealing all the time, and baking temperature is low, and the process gentleness can improve the content of active component in the product to greatest extent; Vacuum belt type drying is particularly suitable for the viscosity height, easily conglomeration, the material of thermoplasticity and thermal sensitivity, Herba Epimedii during drug composition is write out a prescription in the present invention, Semen Cuscutae, Ootheca Mantidis, Fructus Cnidii, Herba Cynomorii, Fructus Ligustri Lucidi, contain the glucosides constituents in the extracting solution of Radix Rehmanniae Preparata and Fructus Rubi eight flavor medical materials, the active component icariin of Herba Epimedii can increase the cardiovascular and cerebrovascular vessel blood flow, promote hemopoietic function, immunologic function and bone metabolism, has kidney invigorating and YANG supporting, defying age, effects such as antitumor, but because the existence of these compositions, the viscosity of extracting solution is big especially, adopt spray drying, the situation of sticking wall can appear in a large amount of extractum, cause the loss of extractum, reduce the quality of preparation, the present invention adopts the vacuum belt type drying technology, not only reduced the loss of extractum, improved the content of active component in the preparation, also improved production efficiency greatly, increase rate has improved 47% than spray drying, and the content of dried cream powder icariin active component has improved 50%.
Find by assay, technology according to prior art, even Cornu Cervi Pantotrichum and Hippocampus have passed through the processing that an alcohol extraction and twice water are proposed, still contain a certain amount of protein in the medicinal residues, the preparation (medicine A) of prior art compositions and the preparation (medicine B) of the present composition are carried out assay and pharmacological testing comparison, the result shows that medicine B obviously improves than the relative amount of medicine A gross protein, increase rate is 37%, aspect the physiology of regulating insufficiency of kidney-YANG and deficiency of the kidney yin animal pattern, medicine B has also shown stronger antagonism insufficiency of kidney-YANG and the effect of deficiency of the kidney yin, is illustrated from the experimental result of assay and pharmacological evaluation two aspects respectively below:
One, assay
1. the assay of Cornu Cervi Pantotrichum, Hippocampus dried cream powder and medicinal residues micropowder
Get Cornu Cervi Pantotrichum, Hippocampus is an amount of, extracting method according to prior art, extracted 24 hours with 75% alcohol dipping of 7 times of medical material amounts earlier, medicinal residues are used water extraction 2 times again, each 2 hours, alcohol extract and water extract were made dried cream powder respectively, and the medicinal residues after water is carried are ground into mean diameter less than the powder of 10 μ m, collect the alcohol extraction dried cream powder respectively, water is carried dried cream powder and medicinal residues micropowder, measures content according to following method:
Get the alcohol extraction dried cream powder, water is carried dried cream powder and each 0.5g of medicinal residues micropowder, accurate claims surely, moves in the dry 500ml kjeldahl flask, adds potassium sulfate 10g and copper sulphate powder 0.5g successively, slowly adds sulphuric acid 20ml along a bottle wall again; Put a little funnel and make kjeldahl flask at 45 tilting at the kjeldahl flask mouth, slowly heat with straight fire, the temperature of solution is remained on below the boiling point, stop Deng intumescence, be heated to boiling, treat that solution is clear and bright green after, continue heating 30 minutes, put cold, along the bottle wall slowly add water 250ml, jolting makes mixing, put cold after, add 40% sodium hydroxide solution 75ml, add zinc granule number, with the nitrogen ball kjeldahl flask is connected with condensing tube; Other gets 2% boric acid solution 50ml, put in the 500ml conical flask, methylate is red-and bromocresol green mixes 10 of indicator solutions, the lower end of condensing tube is inserted under the liquid level of boric acid solution, swing kjeldahl flask gently, make the solution mix homogeneously, add thermal distillation, when being about 250ml to the cumulative volume that receives liquid, with the most advanced and sophisticated liquid level that proposes of condensing tube, made steam flush about 1 minute, with stopping distillation behind the water wash tip, distillate becomes gray purple with sulphuric acid volumetric solution (0.05mol/L) titration to solution by aeruginous, and the result of titration is proofreaied and correct with blank assay.Every 1ml sulphuric acid volumetric solution (0.05mol/L) is equivalent to the N of 1.401mg.The conversion coefficient of N and protein is 6.25, and experimental result sees Table 1.
Total protein content is measured in table 1 dried cream powder and the medicinal residues
| Detect sequence number | Total protein content in the alcohol extraction dried cream powder (%) | Water is carried total protein content in the dried cream powder (%) | Total protein content in the medicinal residues (%) |
| 1 | 20 | 22 | 18 |
| 2 | 19 | 24 | 17 |
| 3 | 23 | 23 | 20 |
| 4 | 22 | 26 | 19 |
| 5 | 23 | 25 | 20 |
| 6 | 21 | 22 | 18 |
| 7 | 20 | 26 | 22 |
| 8 | 19 | 22 | 19 |
| 9 | 23 | 24 | 18 |
| 10 | 22 | 23 | 20 |
| On average | 21.2 | 23.7 | 19.1 |
Annotate: above result calculated is in the dried cream powder or medicinal residues micropowder of Unit Weight, and the total protein quality is with respect to the relative percentage composition of Cornu Cervi Pantotrichum with Hippocampus medical material total amount.
From above-mentioned experimental result as can be seen, with still containing amounts of protein in the Cornu Cervi Pantotrichum after the prior art extraction and the Hippocampus medicinal residues, if medicinal residues are directly abandoned, just caused the waste of herb resource.
2. active component content is measured in prior art and the medicine of the present invention
Technology according to prior art prepares medicine A, and the technology according to the embodiment of the invention 6 prepares medicine B again, measures the percentage composition of gross protein respectively according to following method:
Precision is measured medicine A and each 10ml of medicine B, moves in the dry 500ml kjeldahl flask, adds potassium sulfate 10g and copper sulphate powder 0.5g successively, slowly adds sulphuric acid 20ml along the bottle wall again; Put a little funnel and make kjeldahl flask at 45 tilting at the kjeldahl flask mouth, slowly heat with straight fire, the temperature of solution is remained on below the boiling point, stop Deng intumescence, be heated to boiling, treat that solution is clear and bright green after, continue heating 30 minutes, put cold, along the bottle wall slowly add water 250ml, jolting makes mixing, put cold after, add 40% sodium hydroxide solution 75ml, add zinc granule number, with the nitrogen ball kjeldahl flask is connected with condensing tube; Other gets 2% boric acid solution 50ml, put in the 500ml conical flask, methylate is red-and bromocresol green mixes 10 of indicator solutions, the lower end of condensing tube is inserted under the liquid level of boric acid solution, swing kjeldahl flask gently, make the solution mix homogeneously, add thermal distillation, when being about 250ml to the cumulative volume that receives liquid, with the most advanced and sophisticated liquid level that proposes of condensing tube, made steam flush about 1 minute, with stopping distillation behind the water wash tip, distillate becomes gray purple with sulphuric acid volumetric solution (0.05mol/L) titration to solution by aeruginous, and the result of titration proofreaied and correct with blank assay, every 1ml sulphuric acid volumetric solution (0.05mol/L) is equivalent to the N of 1.401mg, and the conversion coefficient of N and protein is 6.25, and experimental result sees Table 2 and table 3.
Total protein content is measured among the table 2 medicine A
| Sample | Total protein content (%) |
| Medicine A-1 | 36 |
| Medicine A-2 | 32 |
| Medicine A-3 | 34 |
| Medicine A-4 | 38 |
| Medicine A-5 | 31 |
| Medicine A-6 | 35 |
| Medicine A-7 | 32 |
| Medicine A-8 | 35 |
| Medicine A-9 | 34 |
| Medicine A-10 | 30 |
| On average | 33.7 |
Total protein content is measured among the table 3 medicine B
| Sample | Total protein content (%) |
| Medicine B-1 | 47 |
| Medicine B-2 | 49 |
| Medicine B-3 | 45 |
| Medicine B-4 | 48 |
| Medicine B-5 | 42 |
| Medicine B-6 | 49 |
| Medicine B-7 | 43 |
| Medicine B-8 | 46 |
| Medicine B-9 | 48 |
| Medicine B-10 | 44 |
| On average | 46.1 |
Annotate: above result calculated is in the unit formulation, and the total protein quality is with respect to the relative percentage composition of Cornu Cervi Pantotrichum with Hippocampus medical material total amount.
From above-mentioned experimental result as can be seen, because Cornu Cervi Pantotrichum, Hippocampus medicinal residues have been joined in the compositions after pulverizing, the relative amount of the relative amount of protein protein in the medicine A among the medicine B, the extracting method that prior art is described can not fully extract the protein in Cornu Cervi Pantotrichum and the Hippocampus, if use method of the present invention instead, can guarantee that then the protein in Cornu Cervi Pantotrichum and the Hippocampus is fully used.
3. the comparison of vacuum belt type drying and spray drying technology
Herba Epimedii, Semen Cuscutae, Ootheca Mantidis, Fructus Cnidii, Herba Cynomorii, Fructus Ligustri Lucidi, Radix Rehmanniae Preparata and Fructus Rubi eight flavor medical materials are according to prior art, twice of extracting in water, extracting solution merges, filter, filtrate is made extractum, extractum prepares dried cream powder A with the vacuum belt type drying technology respectively, prepares dried cream powder B with spray drying technology.Dried cream powder is measured content Determination of Icariin according to following method.
Test according to high performance liquid chromatography: be filler with the octadecylsilane chemically bonded silica; Be mobile phase with the acetonitrile-water; The detection wavelength is 270nm.Number of theoretical plate calculates by the icariin peak should be not less than 1500, the preferred 30:70 of described acetonitrile-water ratio.
It is an amount of to get the icariin reference substance, and accurate the title decides, and adds methanol and makes the solution that every 1ml contains icariin 0.1mg, namely gets reference substance solution.
Get dried cream powder A and each 0.5g of dried cream powder B, the accurate title, decide, and the accurate methanol 25ml that adds claims to decide weight, be that 250W, frequency are supersound process 30 minutes under the condition of 20kHz at power, take out, put and be chilled to room temperature, claim to decide weight again, supply the weight that subtracts mistake with methanol, filter, get subsequent filtrate, namely get need testing solution.
Accurate reference substance solution and each 10 μ l of need testing solution of drawing inject chromatograph of liquid respectively, measure peak area, calculate content Determination of Icariin.Measurement result sees Table 4.
The relative percentage composition of icariin among the table 4 dried cream powder A
| Dried cream powder | Icariin percentage composition (%) |
| Dried cream powder A-1 | 0.25 |
| Dried cream powder A-2 | 0.26 |
| Dried cream powder A-3 | 0.23 |
| Dried cream powder A-4 | 0.26 |
| Dried cream powder A-5 | 0.24 |
| Dried cream powder A-6 | 0.25 |
| Dried cream powder A-7 | 0.22 |
| Dried cream powder A-8 | 0.23 |
| Dried cream powder A-9 | 0.24 |
| Dried cream powder A-10 | 0.23 |
| On average | 0.24 |
The relative percentage composition of icariin among the table 5 dried cream powder B
| Dried cream powder | Icariin percentage composition (%) |
| Dried cream powder B-1 | 0.16 |
| Dried cream powder B-2 | 0.16 |
| Dried cream powder B-3 | 0.15 |
| Dried cream powder B-4 | 0.16 |
| Dried cream powder B-5 | 0.17 |
| Dried cream powder B-6 | 0.15 |
| Dried cream powder B-7 | 0.15 |
| Dried cream powder B-8 | 0.16 |
| Dried cream powder B-9 | 0.14 |
| Dried cream powder B-10 | 0.17 |
| On average | 0.16 |
Annotate: above result calculated is that icariin is with respect to the relative percentage composition of Herba Epimedii crude drug.
By above experimental data as can be known, compare with the dried cream powder B that spray drying makes, dried cream powder A is owing to adopted the technology of vacuum belt type drying, and content Determination of Icariin has improved 50%.As seen, adopt the vacuum belt type drying technology to prepare dried cream powder, can obviously improve the content of active component in the medicine.
Two, pharmacological experiment
Technology according to prior art prepares medicine A, and the technology according to the embodiment of the invention 6 and embodiment 7 prepares medicine B1 and medicine B2 respectively again, carries out pharmacology test according to following method respectively.
1. compositions is to the physiological influence of insufficiency of kidney-YANG rat:
Modeling method and grouping: 100 of Wistar male rats, be divided into 5 groups at random, 20 every group, 1. blank group: every 9:00 intramuscular injection every day normal saline 0.4ml, played every day every 17:00 on the 1st and irritate stomach with the 1ml drinking water; 2. insufficiency of kidney-YANG model control group: every 9:00 intramuscular injection every day hydrocortisone acetate (5mg/ml) 0.4ml, played every day every 17:00 on the 1st and irritate stomach with the 1ml drinking water; 3. medicine A group: every 9:00 intramuscular injection every day hydrocortisone acetate (5mg/ml) 0.4ml, played every day every 17:00 on the 1st and irritate stomach with medicine A according to the dosage of 10ml/kg; 4. medicine B1 group: every 9:00 intramuscular injection every day hydrocortisone acetate (5mg/ml) 0.4ml, played every day every 17:00 on the 1st and irritate stomach with medicine B1 according to the dosage of 10ml/kg; 5. medicine B2 group: every 9:00 intramuscular injection every day hydrocortisone acetate (5mg/ml) 0.4ml, played every day every 17:00 on the 1st and irritate stomach, successive administration 24 days with medicine B2 solution according to the dosage of 8g/kg.
After the administration 24 days, the electric stimulating instrument stimulating electrode is positioned over rat penis position, give 4mA, the stimulation of 25V voltage, record erection incubation period (stimulate certainly and begin to erect the time to penis), weighing next day the weight of animals is plucked the eyeball blood sampling, puts to death animal, win preputial glands, seminal fluid capsule-prostate, levator ani, hypophysis, the adrenal gland, thymus, spleen, claim weight in wet base with electronic balance, calculate organ index (internal organs weight/body constitution amount), serum measured by radioimmunoassay testosterone (T), lutropin (LH), follicule-stimulating hormone (FSH) (FSH), hydrocortisone (F) content, experimental result sees Table 6.
Table 6 compositions is to the physiological function influence of insufficiency of kidney-YANG rat
 |
The normal control group | The insufficiency of kidney-YANG model control group | Medicine A group | Medicine B1 group | Medicine B2 group |
| Erection incubation period (s) | 23.7±2.1 | 30.9±5.2 | 26.6±2.8 | 24.1±2.7** | 23.3±3.1** |
| Preputial glands index (μ g/g) | 312.0±55.8 | 207.4±30.6 | 258.3±46.2 | 283.4±40.6* | 286.8±50.2* |
| Seminal fluid capsule-prostate index (μ g/g) | 2337.6±157.2 | 328.4±60.1 | 430.6±52.4 | 465.8±57.2** | 461.9±57.2** |
| Levator ani index (μ g/g) | 537.8±47.6 | 238.5±37.2 | 279.6±38.5 | 288.6±38.5* | 291.7±51.9* |
| T(nmol/L) | 3.15±0.62 | 0.26±0.09 | 0.41±0.12 | 0.55±0.09* | 0.57±0.11* |
| F(nmol/L) | 36.20±5.31 | 24.61±4.83 | 27.59±5.76 | 31.70±3.19** | 32.98±6.27** |
| LH(IU/L) | 0.83±0.45 | 1.51±0.32 | 1.18±0.40 | 1.09±0.28* | 1.13±0.51* |
| FSH(IU/L) | 1.82±0.73 | 2.95±0.67 | 2.24±0.55 | 2.10±0.36** | 2.08±0.49** |
Annotate: compare * P＜0.05, * * P＜0.01 with medicine A group
Draw with the SAS8.0 software statistics: in above-mentioned eight groups of data, the P of medicine B1 group and medicine B2 group〉0.05, there was no significant difference.In erection incubation period, seminal fluid capsule-prostate index, F concentration, four groups of experimental datas of FSH concentration, P＜0.01 of medicine B1 group, medicine B2 group and medicine A group, and all with the insufficiency of kidney-YANG model control group significant difference is arranged, in levator ani index, T concentration, LH concentration, four groups of data of preputial glands index, P＜0.05 of medicine B1 group, medicine B2 group and medicine A group, and all with the insufficiency of kidney-YANG model control group significant difference is arranged, illustrate that medicine B can regulate the physiological function of insufficiency of kidney-YANG rat more significantly.
2. compositions is to the physiological influence of deficiency of the kidney yin mice:
Modeling method and grouping: 100 of ICR kind mices, male and female half and half are divided into 5 groups at random, and 20 every group, 1. blank group: every 9:00 intramuscular injection every day normal saline 0.4ml, played every day every 17:00 on the 1st and irritate stomach with the 1ml drinking water; 2. deficiency of the kidney yin model control group: every day, every 9:00 irritated stomach thyroxine sheet (150mg/kg) and reserpine (1mg/kg), played every day every 17:00 on the 1st with 1ml drinking water filling stomach; 3. medicine A group: every day, every 9:00 irritated stomach thyroxine sheet (150mg/kg) and reserpine (1mg/kg), played every day every 17:00 on the 1st with the dosage filling stomach of medicine A according to 10ml/kg; 4. medicine B1 group: every day, every 9:00 irritated stomach thyroxine sheet (150mg/kg) and reserpine (1mg/kg), played every day every 17:00 on the 1st with the dosage filling stomach of medicine B1 according to 10ml/kg; 5. medicine B2 group: every day, every 9:00 irritated stomach thyroxine sheet (150mg/kg) and reserpine (1mg/kg), played every day every 17:00 on the 1st with the dosage filling stomach of medicine B2 solution according to 8g/kg, successive administration 7 days.
Before putting to death mice on the 8th day, measure mice body constitution amount, measure the weight of mice rate, after putting to death mice, win eyeball and get blood, measure blood plasma adenosine cyclophosphate (cAMP), cyclic GMP (cGMP), trilute (T3) and thyroxine (T4) respectively, and calculate cAMP/cGMP.Win thymus, spleen, calculate thymus and index and spleen index: organ index=internal organs quality/mice body constitution amount, experimental result sees Table 7-9.
Table 7 compositions is to the influence of deficiency of the kidney yin mice organ index
 |
The blank group | The deficiency of the kidney yin matched group | Medicine A group | Medicine B1 group | Medicine B2 group |
| Body weight gain rate (%) | 35.18±2.64 | 3.67±2.89 | 27.37±2.01 | 30.95±1.82* | 32.19±3.05* |
| Thymus index (mg/g) | 0.29±0.28 | 0.19±0.11 | 0.23±0.15 | 0.25±0.12 | 0.27±0.16 |
| Spleen index (mg/g) | 0.66±0.37 | 0.50±0.28 | 0.54±0.43 | 0.61±0.20* | 0.63±0.32* |
Annotate: compare * P＜0.05 with medicine A group
With the SAS8.0 statistical software above-mentioned data are carried out statistical analysis, the result shows in three groups of data, P＜0.01 of medicine A group, medicine B1 group, medicine B2 group and deficiency of the kidney yin matched group, illustrate that medicine A, medicine B1 and medicine B2 all have the effect for the treatment of deficiency of the kidney yin, simultaneously, in two groups of data of body weight gain rate and spleen index, medicine B1 group and medicine B2 group and P＜0.05 that medicine A organizes have significant difference.
Table 8 compositions is to the influence of deficiency of the kidney yin mice cAMP, cGMP
Annotate: compare * P＜0.05 with medicine A group
With the above-mentioned three groups of data of SAS8.0 software statistics, medicine A group, medicine B1 group, medicine B2 group all have significant difference with the deficiency of the kidney yin matched group, and in three groups of data, P＜0.05 of medicine B1 group, medicine B2 group and medicine A group, has significant difference, the drug effect that medicine B is described is better than medicine A, and namely technology of the present invention is better than prior art.
Table 9 compositions is to the influence of deficiency of the kidney yin mice T3, T4
Annotate: compare * P＜0.05, * * P＜0.01 with medicine A group
With the above-mentioned two groups of data of SAS8.0 software statistics, medicine A group, medicine B1 group, medicine B2 group all have significant difference with the deficiency of the kidney yin matched group.In the T3 group data, P＜0.05 of medicine B1 group, medicine B2 group and medicine A group.In the T4 group data, P＜0.01 of medicine B1 group, medicine B2 group and medicine A group.In two groups of data, medicine B1 group, medicine B2 group have significant difference with medicine A group, illustrate that the drug effect of medicine B is better than medicine A, and namely technology of the present invention is better than prior art.
By above pharmacological evaluation as can be seen, medicine B1, medicine B2 and medicine A be the energy warming the kidney to activate YANG all, the YIN nourishing of reinforcing the kidney, and the drug effect of medicine B1 and medicine B2 is better than medicine A.Analyze reason, be because the present invention improves the mode of being used as medicine of Cornu Cervi Pantotrichum and Hippocampus two flavor medical materials, owing to still contain the amounts of protein composition in Cornu Cervi Pantotrichum, the Hippocampus medicinal residues, these compositions have with prior art preparation function and cure mainly consistent pharmacologically active, thereby use Cornu Cervi Pantotrichum, Hippocampus medicinal residues micropowder to produce better therapeutic effect in the present composition.Simultaneously, the Chinese medicine composition that treatment of the present invention is suffered from a deficiency of the kidney adopts concentrating under reduced pressure to be prepared into the extractum except making oral liquid, the granule of making, tablet, powder, capsule, soft capsule, pill and drop pill all are that extractum further is dried to dried cream powder, the vacuum belt type drying technology that adopts can make extractum carry out drying under lower temperature, the loss of effective ingredient in preparation process in other medical material extractum avoided in these improvement, not only bring into play the maximum effect of medical material, and improved the drug effect of composite preparation.
The specific embodiment
Following examples are intended to illustrate the present invention, rather than restriction the present invention.
Embodiment 1: the preparation of capsule
Prescription:
Herba Epimedii 1000g, Fructus Cnidii 1000g, Herba Cistanches 1000g, Fructus Ligustri Lucidi 800g, Radix Rehmanniae Preparata 750g, Fructus Schisandrae Chinensis 600g, Herba Cynomorii 500g, Radix Morindae Officinalis 500g, Semen Cuscutae 400g, Fructus Rubi 400g, Fructus Psoraleae 400g, Radix Ginseng 300g, Ootheca Mantidis 300g, Cornu Cervi Pantotrichum 25g, Hippocampus 25g, starch 200g, microcrystalline Cellulose 800g, silicon dioxide 50g, Pulvis Talci 50g.
Method for making:
Cornu Cervi Pantotrichum, Hippocampus were extracted 24 hours with 85% alcohol dipping of 6 times of medical material amounts, filter, filtrate is at 50 ℃ of temperature, steam pressure 0.12MPa, being concentrated into relative density under the condition of vacuum 0.02MPa is 1.10 ~ 1.15(30 ℃) extractum, extractum is at 55 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, obtain Cornu Cervi Pantotrichum, Hippocampus dried cream powder; Medicinal residues oven dry back after extracting is pulverized, cross 80 mesh sieves, obtain fine powder, fine powder uses the air-flow superfine communication technique at charging rate 20kg/h again, carries out micronizing under the air pressure 0.6MPa condition, makes the medicinal residues micropowder of mean diameter 1 ~ 10 μ m.
Radix Ginseng extracting in water 2 times, the water that add 12 times of medical material amounts the first time extracted 2 hours; Add for the second time the water of 6 times of medical material amounts, extracted 2 hours; Merge extractive liquid,, filter, filtrate is at 90 ℃ of temperature, steam pressure 0.05MPa, being concentrated into relative density under the condition of vacuum 0.09MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 50 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 10L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 40 mesh sieves, obtain the Radix Ginseng dried cream powder.
Herba Cistanches, Radix Morindae Officinalis, Fructus Psoraleae and Fructus Schisandrae Chinensis are made in the rearmounted extraction vessel only, 65% alcohol dipping of 4 times of medical material amounts of adding 72 hours, collect lixiviating solution, filter, filtrate is 50 ℃ of temperature, steam pressure 0.04MPa, being concentrated into relative density under the condition of vacuum 0.09MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 55 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 40 mesh sieves, be designated as dried cream powder No. 1.
Herba Epimedii, Semen Cuscutae, Ootheca Mantidis, Fructus Cnidii, Herba Cynomorii, Fructus Ligustri Lucidi, Radix Rehmanniae Preparata, Fructus Rubi are made in the rearmounted extraction vessel only, extracting in water 2 times, and the water that add 8 times of medical material amounts the first time extracted 2 hours; Add for the second time the water of 4 times of medical material amounts, extracted 2 hours; Merging filtrate, filtrate is at 60 ℃ of temperature, steam pressure 0.05MPa, being concentrated into relative density under the condition of vacuum 0.09MPa is 1.12 ~ 1.16(85 ℃) thick paste, thick paste is put into settling tank, adds 95% ethanol of 3 times of thick paste amounts, stir, left standstill 24 hours, filter; Filtrate is 60 ℃ of temperature, steam pressure 0.05MPa, being condensed into relative density under the condition of vacuum 0.09MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 50 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 40 mesh sieves, be designated as dried cream powder No. 2.
Get above-mentioned Cornu Cervi Pantotrichum, Hippocampus dried cream powder, Cornu Cervi Pantotrichum, Hippocampus medicinal residues micropowder, Radix Ginseng dried cream powder, No. 1 dried cream powder, No. 2 dried cream powders add starch, microcrystalline Cellulose, silicon dioxide and Pulvis Talci, and mix homogeneously is granulated, drying, granulate is filled, and makes the capsule of Chinese medicine composition of the present invention.
Embodiment 2: the preparation of tablet
Prescription:
Herba Epimedii 800g, Fructus Cnidii 800g, Herba Cistanches 800g, Fructus Ligustri Lucidi 700g, Radix Rehmanniae Preparata 650g, Fructus Schisandrae Chinensis 500g, Herba Cynomorii 400g, Radix Morindae Officinalis 400g, Semen Cuscutae 300g, Fructus Rubi 300g, Fructus Psoraleae 300g, Radix Ginseng 200g, Ootheca Mantidis 250g, Cornu Cervi Pantotrichum 20g, Hippocampus 20g, starch 400g, microcrystalline Cellulose 500g, carboxymethyl starch sodium 50g, magnesium stearate 25g, Pulvis Talci 25g.
Method for making:
Cornu Cervi Pantotrichum, Hippocampus were extracted 24 hours with 65% alcohol dipping of 8 times of medical material amounts, filter, filtrate is at 80 ℃ of temperature, steam pressure 0.04MPa, being concentrated into relative density under the condition of vacuum 0.02MPa is 1.10 ~ 1.15(30 ℃) extractum, extractum is at 40 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, obtain Cornu Cervi Pantotrichum, Hippocampus dried cream powder; Medicinal residues oven dry back after extracting is pulverized, cross 80 mesh sieves, obtain fine powder, fine powder uses the air-flow superfine communication technique at charging rate 20kg/h again, carries out micronizing under the air pressure 0.6MPa condition, makes the medicinal residues micropowder of mean diameter 1 ~ 10 μ m.The medicinal residues micropowder
Radix Ginseng extracting in water 2 times, the water that add 8 times of medical material amounts the first time extracted 2 hours; Add for the second time the water of 8 times of medical material amounts, extracted 2 hours; Merge extractive liquid,, filter, filtrate is at 60 ℃ of temperature, steam pressure 0.15MPa, being concentrated into relative density under the condition of vacuum 0.02MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 75 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 10L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, obtain the Radix Ginseng dried cream powder.
Herba Cistanches, Radix Morindae Officinalis, Fructus Psoraleae and Fructus Schisandrae Chinensis are made in the rearmounted extraction vessel only, 85% alcohol dipping of 3 times of medical material amounts of adding 72 hours, collect lixiviating solution, filter, filtrate is 80 ℃ of temperature, steam pressure 0.04MPa, being concentrated into relative density under the condition of vacuum 0.02MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 40 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, be designated as dried cream powder No. 1.
Herba Epimedii, Semen Cuscutae, Ootheca Mantidis, Fructus Cnidii, Herba Cynomorii, Fructus Ligustri Lucidi, Radix Rehmanniae Preparata, Fructus Rubi are made in the rearmounted extraction vessel only, extracting in water 2 times, and the water that add 6 times of medical material amounts the first time extracted 2 hours, added the water of 6 times of medical material amounts for the second time, extracted 2 hours.Merging filtrate, filtrate is at 90 ℃ of temperature, steam pressure 0.05MPa, being concentrated into relative density under the condition of vacuum 0.02MPa is 1.12 ~ 1.16(85 ℃) thick paste, thick paste is put into settling tank, adds 95% ethanol of 3 times of thick paste amounts, stir, left standstill 24 hours, filter; Filtrate is 90 ℃ of temperature, steam pressure 0.05MPa, being condensed into relative density under the condition of vacuum 0.02MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 75 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, be designated as dried cream powder No. 2.
Get above-mentioned Cornu Cervi Pantotrichum, Hippocampus dried cream powder, Cornu Cervi Pantotrichum, Hippocampus medicinal residues micropowder, Radix Ginseng dried cream powder, No. 1 dried cream powder, No. 2 dried cream powders, add starch, microcrystalline Cellulose, carboxymethyl starch sodium, magnesium stearate and Pulvis Talci, mix homogeneously is granulated, dry, granulate, tabletting makes the tablet of Chinese medicine composition of the present invention.
Embodiment 3: the preparation of soft capsule
Prescription:
Herba Epimedii 1200g, Fructus Cnidii 1200g, Herba Cistanches 1200g, Fructus Ligustri Lucidi 900g, Radix Rehmanniae Preparata 850g, Fructus Schisandrae Chinensis 700g, Herba Cynomorii 600g, Radix Morindae Officinalis 600g, Semen Cuscutae 500g, Fructus Rubi 500g, Fructus Psoraleae 500g, Radix Ginseng 350g, Ootheca Mantidis 350g, Cornu Cervi Pantotrichum 30g, hippocampus 3 0g, gelatin 700g, glycerol 300g, ethyl hydroxybenzoate 2g, titanium dioxide 2g
Method for making:
Cornu Cervi Pantotrichum, Hippocampus were extracted 24 hours with 75% alcohol dipping of 7 times of medical material amounts, filter, filtrate is at 60 ℃ of temperature, steam pressure 0.06MPa, being concentrated into relative density under the condition of vacuum 0.04MPa is 1.10 ~ 1.15(30 ℃) extractum, extractum is at 50 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, obtain Cornu Cervi Pantotrichum, Hippocampus dried cream powder; Medicinal residues oven dry back after extracting is pulverized, cross 80 mesh sieves, obtain fine powder, fine powder uses the air-flow superfine communication technique at charging rate 20kg/h again, carries out micronizing under the air pressure 0.6MPa condition, makes the medicinal residues micropowder of mean diameter 1 ~ 10 μ m.The medicinal residues micropowder
Radix Ginseng extracting in water 2 times.Add for the first time the water of 10 times of medical material amounts, extracted 2 hours, add the water of 6 times of medical material amounts for the second time, extracted 2 hours.Merge extractive liquid,, filter, filtrate is at 70 ℃ of temperature, steam pressure 0.1MPa, being concentrated into relative density under the condition of vacuum 0.05MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 60 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 10L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 80 mesh sieves, obtain the Radix Ginseng dried cream powder.
Herba Cistanches, Radix Morindae Officinalis, Fructus Psoraleae and Fructus Schisandrae Chinensis are made in the rearmounted extraction vessel only, 75% alcohol dipping of 2 times of medical material amounts of adding 72 hours, collect lixiviating solution, filter, filtrate is 80 ℃ of temperature, steam pressure 0.12MPa, being concentrated into relative density under the condition of vacuum 0.02MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 45 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 80 mesh sieves, be designated as dried cream powder No. 1.
Herba Epimedii, Semen Cuscutae, Ootheca Mantidis, Fructus Cnidii, Herba Cynomorii, Fructus Ligustri Lucidi, Radix Rehmanniae Preparata, Fructus Rubi are made in the rearmounted extraction vessel only, extracting in water 2 times, and the water that add 8 times of medical material amounts the first time extracted 2 hours, added the water of 6 times of medical material amounts for the second time, extracted 2 hours.Merging filtrate, filtrate is at 70 ℃ of temperature, steam pressure 0.1MPa, being concentrated into relative density under the condition of vacuum 0.05MPa is 1.12 ~ 1.16(85 ℃) thick paste, thick paste is put into settling tank, adds 95% ethanol of 3 times of thick paste amounts, stir, left standstill 24 hours, filter; Filtrate is 70 ℃ of temperature, steam pressure 0.1MPa, being condensed into relative density under the condition of vacuum 0.05MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 60 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 80 mesh sieves, be designated as dried cream powder No. 2.
Get above-mentioned Cornu Cervi Pantotrichum, Hippocampus dried cream powder, Cornu Cervi Pantotrichum, Hippocampus medicinal residues micropowder, the Radix Ginseng dried cream powder, No. 1 dried cream powder, No. 2 dried cream powder mix homogeneously sieve, and with gelatin, glycerol, titanium dioxide and ethyl hydroxybenzoate pill, make the soft capsule of Chinese medicine composition of the present invention.
Embodiment 4: the preparation of powder
Prescription:
Herba Epimedii 800g, Fructus Cnidii 800g, Herba Cistanches 800g, Fructus Ligustri Lucidi 700g, Radix Rehmanniae Preparata 650g, Fructus Schisandrae Chinensis 500g, Herba Cynomorii 400g, Radix Morindae Officinalis 400g, Semen Cuscutae 300g, Fructus Rubi 300g, Fructus Psoraleae 300g, Radix Ginseng 200g, Ootheca Mantidis 250g, Cornu Cervi Pantotrichum 20g, Hippocampus 20g, lactose 1000 g, starch 500g.
Method for making:
Cornu Cervi Pantotrichum, Hippocampus were extracted 24 hours with 65% alcohol dipping of 8 times of medical material amounts, filter, filtrate is at 60 ℃ of temperature, steam pressure 0.08MPa, being concentrated into relative density under the condition of vacuum 0.03MPa is 1.10 ~ 1.15(30 ℃) extractum, extractum is at 55 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, obtain Cornu Cervi Pantotrichum, Hippocampus dried cream powder; Medicinal residues oven dry back after extracting is pulverized, cross 80 mesh sieves, obtain fine powder, fine powder uses the air-flow superfine communication technique at charging rate 20kg/h again, carries out micronizing under the air pressure 0.6MPa condition, makes the medicinal residues micropowder of mean diameter 1 ~ 10 μ m.The medicinal residues micropowder
Radix Ginseng extracting in water 2 times, the water that add 10 times of medical material amounts the first time extracted 2 hours; Add for the second time the water of 8 times of medical material amounts, extracted 2 hours; Merge extractive liquid,, filter, filtrate is at 80 ℃ of temperature, steam pressure 0.07MPa, being concentrated into relative density under the condition of vacuum 0.06MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 55 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 10L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 80 mesh sieves, obtain the Radix Ginseng dried cream powder.
Herba Cistanches, Radix Morindae Officinalis, Fructus Psoraleae and Fructus Schisandrae Chinensis are made in the rearmounted extraction vessel only, 85% alcohol dipping of 4 times of medical material amounts of adding 72 hours, collect lixiviating solution, filter, filtrate is 60 ℃ of temperature, steam pressure 0.05MPa, being concentrated into relative density under the condition of vacuum 0.05MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 50 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, be designated as dried cream powder No. 1.
Herba Epimedii, Semen Cuscutae, Ootheca Mantidis, Fructus Cnidii, Herba Cynomorii, Fructus Ligustri Lucidi, Radix Rehmanniae Preparata, Fructus Rubi are made in the rearmounted extraction vessel only, extracting in water 2 times, and the water that add 7 times of medical material amounts the first time extracted 2 hours, added the water of 5 times of medical material amounts for the second time, extracted 2 hours.Merging filtrate, filtrate is at 80 ℃ of temperature, steam pressure 0.05MPa, being concentrated into relative density under the condition of vacuum 0.08MPa is 1.12 ~ 1.16(85 ℃) thick paste, thick paste is put into settling tank, adds 95% ethanol of 3 times of thick paste amounts, stir, left standstill 24 hours, filter; Filtrate is 80 ℃ of temperature, steam pressure 0.05MPa, being condensed into relative density under the condition of vacuum 0.08MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 70 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, be designated as dried cream powder No. 2.
Get above-mentioned Cornu Cervi Pantotrichum, Hippocampus dried cream powder, Cornu Cervi Pantotrichum, Hippocampus medicinal residues micropowder, the Radix Ginseng dried cream powder, No. 1 dried cream powder, No. 2 dried cream powders add lactose, starch, mixing, drying is pulverized, and sieves, and mixing is made the powder of Chinese medicine composition of the present invention.
Embodiment 5: the preparation of pill
Prescription:
Herba Epimedii 1000g, Fructus Cnidii 1000g, Herba Cistanches 1000g, Fructus Ligustri Lucidi 800g, Radix Rehmanniae Preparata 750g, Fructus Schisandrae Chinensis 600g, Herba Cynomorii 500g, Radix Morindae Officinalis 500g, Semen Cuscutae 400g, Fructus Rubi 400g, Fructus Psoraleae 400g, Radix Ginseng 300g, Ootheca Mantidis 300g, Cornu Cervi Pantotrichum 25g, Hippocampus 25g, polyvidone 400g, water 200g, ethanol 600g.
Method for making:
Cornu Cervi Pantotrichum, Hippocampus were extracted 24 hours with 75% alcohol dipping of 6 times of medical material amounts, filter, filtrate is at 70 ℃ of temperature, steam pressure 0.04MPa, being concentrated into relative density under the condition of vacuum 0.06MPa is 1.10 ~ 1.15(30 ℃) extractum, extractum is at 45 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, obtain Cornu Cervi Pantotrichum, Hippocampus dried cream powder; Medicinal residues oven dry back after extracting is pulverized, cross 80 mesh sieves, obtain fine powder, fine powder uses the air-flow superfine communication technique at charging rate 20kg/h again, carries out micronizing under the air pressure 0.6MPa condition, makes the medicinal residues micropowder of mean diameter 1 ~ 10 μ m.The medicinal residues micropowder
Radix Ginseng extracting in water 2 times, the water that add 12 times of medical material amounts the first time extracted 2 hours; Add for the second time the water of 8 times of medical material amounts, extracted 2 hours; Merge extractive liquid,, filter, filtrate is at 65 ℃ of temperature, steam pressure 0.08MPa, being concentrated into relative density under the condition of vacuum 0.05MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 50 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 10L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, obtain the Radix Ginseng dried cream powder.
Herba Cistanches, Radix Morindae Officinalis, Fructus Psoraleae and Fructus Schisandrae Chinensis are made in the rearmounted extraction vessel only, 65% alcohol dipping of 2 times of medical material amounts of adding 72 hours, collect lixiviating solution, filter, filtrate is 45 ℃ of temperature, steam pressure 0.1MPa, being concentrated into relative density under the condition of vacuum 0.07MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 55 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 80 mesh sieves, be designated as dried cream powder No. 1.
Herba Epimedii, Semen Cuscutae, Ootheca Mantidis, Fructus Cnidii, Herba Cynomorii, Fructus Ligustri Lucidi, Radix Rehmanniae Preparata, Fructus Rubi are made in the rearmounted extraction vessel only, extracting in water 2 times, and the water that add 7 times of medical material amounts the first time extracted 2 hours, added the water of 5 times of medical material amounts for the second time, extracted 2 hours.Merging filtrate, filtrate is at 65 ℃ of temperature, steam pressure 0.06MPa, being concentrated into relative density under the condition of vacuum 0.03MPa is 1.12 ~ 1.16(85 ℃) thick paste, thick paste is put into settling tank, adds 95% ethanol of 3 times of thick paste amounts, stir, left standstill 24 hours, filter; Filtrate is 65 ℃ of temperature, steam pressure 0.06MPa, being condensed into relative density under the condition of vacuum 0.03MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 65 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, be designated as dried cream powder No. 2.
Get above-mentioned Cornu Cervi Pantotrichum, Hippocampus dried cream powder, Cornu Cervi Pantotrichum, Hippocampus medicinal residues micropowder, the Radix Ginseng dried cream powder, No. 1 dried cream powder, No. 2 dried cream powders and polyvidone mix homogeneously sieve, and adopt water, an amount of general ball of ethanol, and drying makes the pill of Chinese medicine composition of the present invention.
Embodiment 6: the preparation of oral liquid
Prescription:
Herba Epimedii 1000g, Fructus Cnidii 1000g, Herba Cistanches 1000g, Fructus Ligustri Lucidi 800g, Radix Rehmanniae Preparata 750g, Fructus Schisandrae Chinensis 600g, Herba Cynomorii 500g, Radix Morindae Officinalis 500g, Semen Cuscutae 400g, Fructus Rubi 400g, Fructus Psoraleae 400g, Radix Ginseng 300g, Ootheca Mantidis 300g, Cornu Cervi Pantotrichum 25g, Hippocampus 25g, sucrose 2000g, potassium sorbate 2g
Method for making:
Cornu Cervi Pantotrichum, Hippocampus extracted 24 hours with 75% alcohol dipping of 7 times of medical material amounts, filtered, and filtrate is 55 ℃ of temperature, and steam pressure 0.05MPa, being condensed into relative density under the condition of vacuum 0.04MPa is 1.10 ~ 1.15(30 ℃) extractum.Medicinal residues oven dry back after extracting is pulverized, cross 80 mesh sieves, obtain fine powder, fine powder uses the air-flow superfine communication technique at charging rate 20kg/h again, carries out micronizing under the air pressure 0.6MPa condition, makes the medicinal residues micropowder of mean diameter 1 ~ 10 μ m.The medicinal residues micropowder
Radix Ginseng extracting in water 2 times, the water that add 10 times of medical material amounts the first time extracted 2 hours; Add for the second time the water of 8 times of medical material amounts, extracted 2 hours; Merge extractive liquid, filters, and filtrate is 65 ℃ of temperature, and steam pressure 0.04MPa, being concentrated into relative density under the condition of vacuum 0.05MPa is 1.25 ~ 1.35(30 ℃) extractum.
Herba Cistanches, Radix Morindae Officinalis, Fructus Psoraleae and Fructus Schisandrae Chinensis are made in the rearmounted extraction vessel only, 75% alcohol dipping of 3 times of medical material amounts of adding 72 hours, collect lixiviating solution, filter, filtrate is 50 ℃ of temperature, steam pressure 0.04MPa, being condensed into relative density under the condition of vacuum 0.05MPa is 1.20 ~ 1.25(30 ℃) extractum, be designated as extractum No. 1.
Herba Epimedii, Semen Cuscutae, Ootheca Mantidis, Fructus Cnidii, Herba Cynomorii, Fructus Ligustri Lucidi, Radix Rehmanniae Preparata, Fructus Rubi are made in the rearmounted extraction vessel only, extracting in water 2 times, and the water that add 7 times of medical material amounts the first time extracted 2 hours; Add for the second time the water of 5 times of medical material amounts, extracted 2 hours; Merging filtrate, filtrate is at 60 ℃ of temperature, steam pressure 0.05MPa, being concentrated into relative density under the condition of vacuum 0.06MPa is 1.12 ~ 1.16(85 ℃) thick paste, thick paste is put into settling tank, adds 95% ethanol of 3 times of thick paste amounts, stir, left standstill 24 hours, filter; Filtrate is 60 ℃ of temperature, and steam pressure 0.05MPa, being condensed into relative density under the condition of vacuum 0.06MPa is 1.25 ~ 1.35(30 ℃) extractum, be designated as extractum No. 2.
Get above-mentioned Cornu Cervi Pantotrichum, Hippocampus extractum, Radix Ginseng extractum, No. 1 extractum, No. 2 extractum and Cornu Cervi Pantotrichum, Hippocampus medicinal residues micropowder add sucrose and potassium sorbate, grind well, and adding distil water is to full dose again, fill, sterilization makes the oral liquid of Chinese medicine composition of the present invention.
Embodiment 7: the preparation of granule
Prescription:
Herba Epimedii 1000g, Fructus Cnidii 1000g, Herba Cistanches 1000g, Fructus Ligustri Lucidi 800g, Radix Rehmanniae Preparata 750g, Fructus Schisandrae Chinensis 600g, Herba Cynomorii 500g, Radix Morindae Officinalis 500g, Semen Cuscutae 400g, Fructus Rubi 400g, Fructus Psoraleae 400g, Radix Ginseng 300g, Ootheca Mantidis 300g, Cornu Cervi Pantotrichum 25g, Hippocampus 25g, lactose 2500g, dextrin 2500g.
Method for making:
Cornu Cervi Pantotrichum, Hippocampus were extracted 24 hours with 75% alcohol dipping of 7 times of medical material amounts, filter, filtrate is at 55 ℃ of temperature, steam pressure 0.05MPa, being concentrated into relative density under the condition of vacuum 0.04MPa is 1.10 ~ 1.15(30 ℃) extractum, extractum is at 50 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, obtain Cornu Cervi Pantotrichum, Hippocampus dried cream powder; Medicinal residues oven dry back after extracting is pulverized, cross 80 mesh sieves, obtain fine powder, fine powder uses the air-flow superfine communication technique at charging rate 20kg/h again, carries out micronizing under the air pressure 0.6MPa condition, makes the medicinal residues micropowder of mean diameter 1 ~ 10 μ m.The medicinal residues micropowder
Radix Ginseng extracting in water 2 times.Add for the first time the water of 10 times of medical material amounts, extracted 2 hours, add the water of 8 times of medical material amounts for the second time, extracted 2 hours.Merge extractive liquid,, filter, filtrate is at 70 ℃ of temperature, steam pressure 0.08MPa, being concentrated into relative density under the condition of vacuum 0.03MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 60 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 10L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, obtain the Radix Ginseng dried cream powder.
Herba Cistanches, Radix Morindae Officinalis, Fructus Psoraleae and Fructus Schisandrae Chinensis are made in the rearmounted extraction vessel only, 75% alcohol dipping of 3 times of medical material amounts of adding 72 hours, collect lixiviating solution, filter, filtrate is 50 ℃ of temperature, steam pressure 0.04MPa, being concentrated into relative density under the condition of vacuum 0.05MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 50 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 80 mesh sieves, be designated as dried cream powder No. 1.
Herba Epimedii, Semen Cuscutae, Ootheca Mantidis, Fructus Cnidii, Herba Cynomorii, Fructus Ligustri Lucidi, Radix Rehmanniae Preparata, Fructus Rubi are made in the rearmounted extraction vessel only, extracting in water 2 times, and the water that add 7 times of medical material amounts the first time extracted 2 hours, added the water of 5 times of medical material amounts for the second time, extracted 2 hours.Merging filtrate, filtrate is at 65 ℃ of temperature, steam pressure 0.06MPa, being concentrated into relative density under the condition of vacuum 0.03MPa is 1.12 ~ 1.16(85 ℃) thick paste, thick paste is put into settling tank, adds 95% ethanol of 3 times of thick paste amounts, stir, left standstill 24 hours, filter; Filtrate is 65 ℃ of temperature, steam pressure 0.06MPa, being condensed into relative density under the condition of vacuum 0.03MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 60 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 80 mesh sieves, be designated as dried cream powder No. 2.
Get above-mentioned Cornu Cervi Pantotrichum, Hippocampus dried cream powder, Cornu Cervi Pantotrichum, Hippocampus medicinal residues micropowder, the Radix Ginseng dried cream powder, No. 1 dried cream powder and No. 2 dried cream powders add lactose, dextrin, and mix homogeneously is granulated, drying, granulate makes the granule of Chinese medicine composition of the present invention.
Embodiment 8: the preparation of drop pill
Prescription:
Herba Epimedii 1200g, Fructus Cnidii 1200g, Herba Cistanches 1200g, Fructus Ligustri Lucidi 900g, Radix Rehmanniae Preparata 850g, Fructus Schisandrae Chinensis 700g, Herba Cynomorii 600g, Radix Morindae Officinalis 600g, Semen Cuscutae 500g, Fructus Rubi 500g, Fructus Psoraleae 500g, Radix Ginseng 350g, Ootheca Mantidis 350g, Cornu Cervi Pantotrichum 30g, hippocampus 3 0g, Polyethylene Glycol 1750g
Method for making:
Cornu Cervi Pantotrichum, Hippocampus were extracted 24 hours with 75% alcohol dipping of 8 times of medical material amounts, filter, filtrate is at 70 ℃ of temperature, steam pressure 0.06MPa, being concentrated into relative density under the condition of vacuum 0.03MPa is 1.10 ~ 1.15(30 ℃) extractum, extractum is at 60 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, obtain Cornu Cervi Pantotrichum, Hippocampus dried cream powder; Medicinal residues oven dry back after extracting is pulverized, cross 80 mesh sieves, obtain fine powder, fine powder uses the air-flow superfine communication technique at charging rate 20kg/h again, carries out micronizing under the air pressure 0.6MPa condition, makes the medicinal residues micropowder of mean diameter 1 ~ 10 μ m.The medicinal residues micropowder
Radix Ginseng extracting in water 2 times.Add for the first time the water of 12 times of medical material amounts, extracted 2 hours, add the water of 10 times of medical material amounts for the second time, extracted 2 hours.Merge extractive liquid,, filter, filtrate is at 80 ℃ of temperature, steam pressure 0.1MPa, being concentrated into relative density under the condition of vacuum 0.02MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 55 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 10L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 80 mesh sieves, obtain the Radix Ginseng dried cream powder.
Herba Cistanches, Radix Morindae Officinalis, Fructus Psoraleae and Fructus Schisandrae Chinensis are made in the rearmounted extraction vessel only, 75% alcohol dipping of 4 times of medical material amounts of adding 72 hours, collect lixiviating solution, filter, filtrate is 70 ℃ of temperature, steam pressure 0.05MPa, being concentrated into relative density under the condition of vacuum 0.07MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 55 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 60 mesh sieves, be designated as dried cream powder No. 1.
Herba Epimedii, Semen Cuscutae, Ootheca Mantidis, Fructus Cnidii, Herba Cynomorii, Fructus Ligustri Lucidi, Radix Rehmanniae Preparata, Fructus Rubi are made in the rearmounted extraction vessel only, extracting in water 2 times, and the water that add 8 times of medical material amounts the first time extracted 2 hours, added the water of 6 times of medical material amounts for the second time, extracted 2 hours.Merging filtrate, filtrate is at 80 ℃ of temperature, steam pressure 0.1MPa, being concentrated into relative density under the condition of vacuum 0.02MPa is 1.12 ~ 1.16(85 ℃) thick paste, thick paste is put into settling tank, adds 95% ethanol of 3 times of thick paste amounts, stir, left standstill 24 hours, filter; Filtrate is 80 ℃ of temperature, steam pressure 0.1MPa, being condensed into relative density under the condition of vacuum 0.02MPa is 1.25 ~ 1.35(30 ℃) extractum, extractum is at 50 ℃ of temperature, vacuum pressure-1.0MPa, charging rate 15L/h, vacuum belt drier is dried to dried cream under the strip transfer rate 25cm/min condition, pulverize, cross 40 ~ 80 mesh sieves, be designated as dried cream powder No. 2.
Get above-mentioned Cornu Cervi Pantotrichum, Hippocampus dried cream powder, Cornu Cervi Pantotrichum, Hippocampus medicinal residues micropowder, the Radix Ginseng dried cream powder, No. 1 dried cream powder and No. 2 dried cream powder mix homogeneously add Polyethylene Glycol, are heated to fusing, and mixing makes the drop pill of Chinese medicine composition of the present invention.
Claims (7)
1. the Chinese medicine composition that treatment is suffered from a deficiency of the kidney is characterized in that Chinese medicine composition is grouped into by following one-tenth: Herba Epimedii 800-1200g, Fructus Cnidii 800-1200g, Herba Cistanches 800-1200g, Fructus Ligustri Lucidi 700-900g, Radix Rehmanniae Preparata 650-850g, Fructus Schisandrae Chinensis 500-700g, Herba Cynomorii 400-600g, Radix Morindae Officinalis 400-600g, Semen Cuscutae 300-500g, Fructus Rubi 300-500g, Fructus Psoraleae 300-500g, Radix Ginseng 200-350g, Ootheca Mantidis 250-350g, Cornu Cervi Pantotrichum 20-30g, Hippocampus 20-30g and pharmaceutically acceptable additives 1000-5000g; Wherein said pharmaceutically acceptable additives are one or more of starch, sucrose, lactose, dextrin, microcrystalline Cellulose, hypromellose, polyvidone, Sodium Hydroxymethyl Stalcs, magnesium stearate, Pulvis Talci, Polyethylene Glycol, micropowder silica gel, vegetable oil, glycerol, paraffin, tween 80, gelatin, titanium dioxide and ethyl hydroxybenzoate;
Preparation as follows:
(1), Radix Ginseng extracting in water 2 times, add the water of 8-12 times of medical material amount for the first time, extracted 2 hours; Add for the second time the water of 6-10 times of medical material amount, extracted 2 hours; Merge extractive liquid, filters, and filtrate concentrating made relative density and further be dried to dried cream at 30 ℃ for the extractum of 1.25-1.35 or with extractum in vacuum belt drier, pulverizes, and crosses the 40-80 mesh sieve, and it is standby to make dried cream powder;
(2), Cornu Cervi Pantotrichum, Hippocampus add the 65-85% ethanol of 6-8 times of medical material amount, dipping extracted 24 hours, filter, relative density was the extractum of 1.10-1.15 or extractum is dried to dried cream in vacuum belt drier when filtrate concentrated and to be formed in 30 ℃, pulverize, cross 60 mesh sieves, it is standby to make dried cream powder;
(3), step (2) is extracted Cornu Cervi Pantotrichum, the oven dry of posthippocampal medicinal residues, pulverizing, 80 mesh sieves excessively, obtain fine powder, fine powder is 10-25kg/h with the air-flowing type superfine communication technique in charging rate again, and air pressure is to carry out micronizing under the condition of 0.5-0.8Mpa, and it is standby to make the medicinal residues micropowder;
(4), Herba Cistanches, Radix Morindae Officinalis, Fructus Psoraleae and Fructus Schisandrae Chinensis are made in the rearmounted extraction vessel only, the 65-85% alcohol dipping that adds 2-4 times of medical material amount was extracted 72 hours, collect lixiviating solution, filter, relative density was the extractum of 1.25-1.35 or extractum further is dried to dried cream in vacuum belt drier when filtrate concentrated and to be formed in 30 ℃, pulverize, cross the 40-80 mesh sieve, it is standby to make dried cream powder;
(5), Herba Epimedii, Semen Cuscutae, Ootheca Mantidis, Fructus Cnidii, Herba Cynomorii, Fructus Ligustri Lucidi, Radix Rehmanniae Preparata, Fructus Rubi make in the rearmounted extraction vessel only, extracting in water 2 times adds the water of 6-8 times of medical material amount for the first time, extracts 2 hours; Add for the second time the water of 4-6 times of medical material amount, extracted 2 hours; Merge extractive liquid, filters, and filtrate is condensed into that relative density is the thick paste of 1.12-1.16 in the time of 85 ℃, thick paste is put into settling tank, add 95% ethanol of 3 times of thick paste amounts, stir, left standstill 24 hours, filter, filtrate decompression concentrates when being formed in 30 ℃ relative density and is the extractum of 1.25-1.35 or extractum further is dried to dried cream in vacuum belt drier, pulverizes, cross the 40-80 mesh sieve, it is standby to make dried cream powder;
(6), extractum or the dried cream powder with step (1), (2), (4), (5) mixes with the medicinal residues micropowder of step (3), add pharmaceutically acceptable additives, make oral liquid, granule, tablet, powder, capsule or pill, namely get the Chinese medicine composition that treatment is suffered from a deficiency of the kidney.
2. the treatment as claimed in claim 1 Chinese medicine composition of suffering from a deficiency of the kidney is characterized in that the filtrate after described Radix Ginseng water is carried is at temperature 60-90 ℃, and steam pressure 0.05-0.15Mpa is condensed into extractum under the condition of vacuum 0.02-0.09Mpa; Or with extractum at temperature 50-75 ℃, vacuum pressure-1.0Mpa, charging rate 10L/h carries out vacuum belt type drying under the strip transfer rate 25cm/min condition and becomes dried cream.
3. the treatment as claimed in claim 1 Chinese medicine composition of suffering from a deficiency of the kidney is characterized in that the filtrate after extracting of described Cornu Cervi Pantotrichum, Hippocampus is at temperature 50-80 ℃, and steam pressure 0.04-0.12Mpa is condensed into extractum under the condition of vacuum 0.02-0.09Mpa; Or with extractum at temperature 40-55 ℃, vacuum pressure-1.0Mpa, charging rate 15L/h carries out vacuum belt type drying under the strip transfer rate 25cm/min condition and becomes dried cream.
4. the treatment as claimed in claim 1 Chinese medicine composition of suffering from a deficiency of the kidney, it is characterized in that extracting Cornu Cervi Pantotrichum, posthippocampal medicinal residues fine powder and be in charging rate is 10-25kg/h, air pressure is to carry out micronizing under the condition of 0.5-0.8Mpa.
5. the treatment as claimed in claim 4 Chinese medicine composition of suffering from a deficiency of the kidney, after it is characterized in that extracting Cornu Cervi Pantotrichum, posthippocampal medicinal residues and carrying out micronizing, the particle diameter of medicinal residues micropowder is 1-10 μ m.
6. the treatment as claimed in claim 1 Chinese medicine composition of suffering from a deficiency of the kidney, it is characterized in that described Herba Cistanches, Radix Morindae Officinalis, Fructus Psoraleae and the Fructus Schisandrae Chinensis filtrate after extracting is at temperature 50-80 ℃, steam pressure 0.04-0.12Mpa is condensed into extractum under the condition of vacuum 0.02-0.09Mpa; Or with extractum at temperature 40-55 ℃, vacuum pressure-1.0Mpa, charging rate 15L/h carries out vacuum belt type drying under the strip transfer rate 25cm/min condition and becomes dried cream.
7. the treatment as claimed in claim 1 Chinese medicine composition of suffering from a deficiency of the kidney, it is characterized in that the extracting solution of described Herba Epimedii, Semen Cuscutae, Ootheca Mantidis, Fructus Cnidii, Herba Cynomorii, Fructus Ligustri Lucidi, Radix Rehmanniae Preparata, Fructus Rubi is at temperature 60-90 ℃, steam pressure 0.05-0.15Mpa is condensed into thick paste under the condition of vacuum 0.02-0.09Mpa; The mixed filtrate of thick paste and ethanol is at temperature 50-90 ℃, steam pressure 0.04-0.12Mpa, and the condition of vacuum 0.02-0.09Mpa is condensed into extractum; Or with extractum at temperature 50-75 ℃, vacuum pressure-1.0Mpa, charging rate 15L/h carries out vacuum belt type drying under the strip transfer rate 25cm/min condition and becomes dried cream.
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| CN104258022A (en) * | 2014-10-13 | 2015-01-07 | 河南羚锐制药股份有限公司 | Production technology of traditional Chinese medicine for treating kidney deficiency and blood-stasis obstruction syndromes in meridian recovery period of ischemic stroke |
| CN105770911A (en) * | 2016-04-20 | 2016-07-20 | 刘树民 | Establishment method of kidney-yin deficiency + nephrotic edema composite model |
| CN106511781A (en) * | 2016-12-19 | 2017-03-22 | 四川科宇实业有限公司 | Water-paste pills |
| CN106620022A (en) * | 2017-01-23 | 2017-05-10 | 邹玉华 | Yin-nourishing kidney-tonifying yang-invigorating traditional Chinese medicine composition |
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