CN105535428A - Method for preparing Tianwang preparation capable of tonifying heart - Google Patents

Method for preparing Tianwang preparation capable of tonifying heart Download PDF

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Publication number
CN105535428A
CN105535428A CN201510988804.1A CN201510988804A CN105535428A CN 105535428 A CN105535428 A CN 105535428A CN 201510988804 A CN201510988804 A CN 201510988804A CN 105535428 A CN105535428 A CN 105535428A
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radix
filter
water
time
powder
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黄文荣
马静
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BAIHUA PHARMACEUTICAL GROUP Co Ltd
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BAIHUA PHARMACEUTICAL GROUP Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/28—Mercury; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A61K36/076—Poria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/13—Coniferophyta (gymnosperms)
    • A61K36/14—Cupressaceae (Cypress family), e.g. juniper or cypress
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/232—Angelica
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/34—Campanulaceae (Bellflower family)
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/34—Campanulaceae (Bellflower family)
    • A61K36/344—Codonopsis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484—Glycyrrhiza (licorice)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/79—Schisandraceae (Schisandra family)
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/80—Scrophulariaceae (Figwort family)
    • A61K36/804—Rehmannia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/80—Scrophulariaceae (Figwort family)
    • A61K36/808—Scrophularia (figwort)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8965—Asparagus, e.g. garden asparagus or asparagus fern
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8968—Ophiopogon (Lilyturf)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/1605—Excipients; Inactive ingredients
    • A61K9/1629—Organic macromolecular compounds
    • A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2022—Organic macromolecular compounds
    • A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
    • A61K9/2059—Starch, including chemically or physically modified derivatives; Amylose; Amylopectin; Dextrin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

Abstract

The invention discloses a method for preparing a Tianwang preparation capable of tonifying the heart. The Tianwang preparation capable of tonifying the heart is mainly prepared from salvia miltiorrhiza, angelica sinensis, rhizoma acori graminei, codonopsis pilosula, poria cocos, fructus schizandrae, radix ophiopogonis, radix asparagi, radix rehmanniae, radix scrophulariae, prepared polygala root, parched wild jujube seed, semen platycladi, radix platycodi, radix glycyrrhizae and cinnabar. According to the method, the specific production process and parameters for the Tianwang preparation capable of tonifying the heart are improved according to the nature of each drug in a prescription of the Tianwang preparation capable of tonifying the heart and the effects of the corresponding drug in the prescription, so that an effective ingredient, i.e., salvianolic acid B of the salvia miltiorrhiza is easy to assay, the content of the salvianolic acid B assayed is high, and thus, the preparation is more refined and is more remarkable in drug effect and good in clinical treatment effect.

Description

A kind of manufacture method of king's reinforcing the heart preparation
Technical field
The invention belongs to the field of Chinese medicines, be specifically related to a kind of manufacture method of king's reinforcing the heart preparation.
Background technology
Chinese medicine extraction is a kind of method conventional in herbal pharmaceutical, and chemical composition of Chinese materia medica and complexity thereof, need extract the effective active ingredient of Chinese herbs of human body from the mixture of complexity.To improve drug effect.
King's reinforcing the heart preparation is made primarily of Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Rhizoma Acori Graminei, Radix Codonopsis, Poria, Fructus Schisandrae Chinensis, Radix Ophiopogonis, Radix Asparagi, Radix Rehmanniae, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi, Radix Platycodonis, Radix Glycyrrhizae and Cinnabaris; There is nourishing YIN and benefiting blood, effect of heart tonifying and tranquilizing, for insufficiency of heart-YIN, palpitation and amnesia, insomnia and dreamful sleep, the disease such as to be hard and dry.
In prior art, king's reinforcing the heart preparation makes like this: the Radix Ophiopogonis of getting Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Radix Codonopsis, Poria, Radix Platycodonis and formula ratio 50%, be ground into fine powder; Cinnabaris water flies into impalpable powder, with above-mentioned powder facing-up; Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decocting liquid filters, and filtrate merges, and is condensed into thick paste; All the other medical materials and remaining Radix Ophiopogonis decoct with water secondary, first time 3h, second time 2h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, mixes, pill with above-mentioned fine powder and thick paste, dry, to obtain final product.But inventor finds in research process, adopt existing extraction process to make in king's reinforcing the heart preparation, the effective ingredient of Radix Salviae Miltiorrhizae is not easily measured, and especially salvianolic acid B is not easily measured, and makes the clinical efficacy of king's reinforcing the heart preparation desirable not enough.
Summary of the invention
The invention provides a kind of manufacture method of king's reinforcing the heart preparation.The present invention is according to the character of each medicine in king's reinforcing the heart preparation recipe, and this medicine role in prescription, the concrete processing technology of king's reinforcing the heart preparation and parameter are improved, the effective ingredient salvianolic acid B of Radix Salviae Miltiorrhizae is easily measured, and the content of danshinolic acid B measured is high, make the refine more of this medicine, drug effect is more remarkable, and clinical efficacy is good.
To achieve these goals, the invention provides following technical scheme: a kind of manufacture method of king's reinforcing the heart preparation, described king's reinforcing the heart preparation calculates by weight, primarily of Radix Salviae Miltiorrhizae 20-30 part, Radix Angelicae Sinensis 40-60 part, Rhizoma Acori Graminei 20-30 part, Radix Codonopsis 20-30 part, Poria 20-30 part, Fructus Schisandrae Chinensis 40-60 part, Radix Ophiopogonis 40-60 part, Radix Asparagi 40-60 part, Radix Rehmanniae 150-250 part, Radix Scrophulariae 20-30 part, Radix Polygalae (processed) 20-30 part, Semen Ziziphi Spinosae (parched) 40-60 part, Semen Platycladi 40-60 part, Radix Platycodonis 20-30 part, Radix Glycyrrhizae 20-30 part and Cinnabaris 5-15 part makes; The manufacture method of described king's reinforcing the heart preparation is: get Radix Salviae Miltiorrhizae, decoct with water, and decocting liquid filters, and is condensed into concentrated solution, filter, supernatant adds ethanol, makes alcohol content reach 60-85%, adjust pH7 ~ 10 with sodium hydroxide solution, cold preservation, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1-3:3-1, adjusts pH5 ~ 7 with hydrochloric acid solution, filter, be condensed into extractum, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use; Get Radix Ophiopogonis, decoct with water, filter, filtrate reduced in volume, to clear paste, adds ethanol and makes alcohol content reach 55%-85%, stir, and leaves standstill 12-48 hour, and filter, decompression filtrate recycling ethanol is also condensed into extractum, dry, pulverize, obtains Radix Ophiopogonis powder, for subsequent use; Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use; Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, obtains Radix Rehmanniae thick paste, for subsequent use; Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, and second time 2h, decocting liquid filters, and filtrate is condensed into thick paste, mixes, add adjuvant, make pharmaceutical preparation with above-mentioned facing-up powder and Radix Rehmanniae thick paste.
In the manufacture method of aforesaid king's reinforcing the heart preparation, described king's reinforcing the heart preparation calculates by weight, makes primarily of Radix Salviae Miltiorrhizae 25 parts, Radix Angelicae Sinensis 50 parts, Rhizoma Acori Graminei 25 parts, Radix Codonopsis 25 parts, 25 parts, Poria, Fructus Schisandrae Chinensis 50 parts, Radix Ophiopogonis 50 parts, Radix Asparagi 50 parts, Radix Rehmanniae 200 parts, Radix Scrophulariae 25 parts, Radix Polygalae (processed) 25 parts, Semen Ziziphi Spinosae (parched) 50 parts, Semen Platycladi 50 parts, Radix Platycodonis 25 parts, 25 parts, Radix Glycyrrhizae and 10 parts, Cinnabaris, the manufacture method of described king's reinforcing the heart preparation is: get Radix Salviae Miltiorrhizae, add 8-15 times amount soak by water 1-4 time, each 0.5-3 hour, collecting decoction, filter, being concentrated into volume every milliliter with the ratio of medical material amount every gram is 1-3:3-1, filter, supernatant adds ethanol, alcohol content is made to reach 60-85%, pH7 ~ 10 are adjusted with 5-20% sodium hydroxide solution, cold preservation, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1-3:3-1, pH5 ~ 7 are adjusted with 5-20% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 7-12 times of soak by water 2-4 time, each 0.5-3 hour, filter, merging filtrate, concentrating under reduced pressure becomes clear paste, adding ethanol makes alcohol content reach 55%-85%, stirs, and leaves standstill 12-48 hour, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, and second time 2h, decocting liquid filters, and filtrate is condensed into thick paste, mixes, add adjuvant, make pharmaceutical preparation with above-mentioned facing-up powder and thick paste.
In the manufacture method of aforesaid king's reinforcing the heart preparation, get Radix Salviae Miltiorrhizae, add 10 times amount soak by water 3 times, each 2 hours, collecting decoction, filter, when to be concentrated into volume every milliliter be 1:1 with the ratio of medical material amount every gram, filter, supernatant adds ethanol, alcohol content is made to reach 70%, pH8 ~ 9 are adjusted with 10% sodium hydroxide solution, cold preservation, cold preservation 36 hours, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1:1, pH5-6 is adjusted with 10% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, and second time 2h, decocting liquid filters, and filtrate is condensed into thick paste, mixes, add adjuvant, make pharmaceutical preparation with above-mentioned facing-up powder and thick paste.
In the manufacture method of aforesaid king's reinforcing the heart preparation, described pharmaceutical preparation is oral formulations.
In the manufacture method of aforesaid king's reinforcing the heart preparation, described oral formulations is pill, tablet, capsule or granule.
In the manufacture method of aforesaid king's reinforcing the heart preparation, described pill makes like this: get Radix Salviae Miltiorrhizae, add 10 times amount soak by water 3 times, each 2 hours, collecting decoction, filter, when to be concentrated into volume every milliliter be 1:1 with the ratio of medical material amount every gram, filter, supernatant adds ethanol, alcohol content is made to reach 70%, pH8 ~ 9 are adjusted with 10% sodium hydroxide solution, cold preservation, cold preservation 36 hours, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1:1, pH5-6 is adjusted with 10% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, and second time 2h, decocting liquid filters, and filtrate is condensed into thick paste, and mix with above-mentioned facing-up powder and thick paste, pill, to obtain final product.
In the manufacture method of aforesaid king's reinforcing the heart preparation, described tablet makes like this: get Radix Salviae Miltiorrhizae, add 10 times amount soak by water 3 times, each 2 hours, collecting decoction, filter, when to be concentrated into volume every milliliter be 1:1 with the ratio of medical material amount every gram, filter, supernatant adds ethanol, alcohol content is made to reach 70%, pH8 ~ 9 are adjusted with 10% sodium hydroxide solution, cold preservation, cold preservation 36 hours, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1:1, pH5-6 is adjusted with 10% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, second time 2h, and decocting liquid filters, filtrate is condensed into thick paste, mixes with above-mentioned facing-up powder and thick paste, then adds the starch mixing of the amount of making 10-20%, tabletting, dry, to obtain final product.
In the manufacture method of aforesaid king's reinforcing the heart preparation, described capsule makes like this: get Radix Salviae Miltiorrhizae, add 10 times amount soak by water 3 times, each 2 hours, collecting decoction, filter, when to be concentrated into volume every milliliter be 1:1 with the ratio of medical material amount every gram, filter, supernatant adds ethanol, alcohol content is made to reach 70%, pH8 ~ 9 are adjusted with 10% sodium hydroxide solution, cold preservation, cold preservation 36 hours, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1:1, pH5-6 is adjusted with 10% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, second time 2h, decocting liquid filters, and filtrate is condensed into thick paste, mix with above-mentioned facing-up powder and thick paste, add the starch mixing of the amount of making 10-20% again, granulate, dry, incapsulate, to obtain final product.
In the manufacture method of aforesaid king's reinforcing the heart preparation, described granule makes like this: get Radix Salviae Miltiorrhizae, add 10 times amount soak by water 3 times, each 2 hours, collecting decoction, filter, when to be concentrated into volume every milliliter be 1:1 with the ratio of medical material amount every gram, filter, supernatant adds ethanol, alcohol content is made to reach 70%, pH8 ~ 9 are adjusted with 10% sodium hydroxide solution, cold preservation, cold preservation 36 hours, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1:1, pH5-6 is adjusted with 10% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, second time 2h, and decocting liquid filters, filtrate is condensed into thick paste, mixes with above-mentioned facing-up powder and thick paste, then adds the starch mixing of the amount of making 10-20%, granulate, dry, to obtain final product.
The present invention adopts Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Rhizoma Acori Graminei, Radix Codonopsis, Poria, Fructus Schisandrae Chinensis, Radix Ophiopogonis, Radix Asparagi, Radix Rehmanniae, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi, Radix Platycodonis, Radix Glycyrrhizae and Cinnabaris to make medicine, i.e. king's reinforcing the heart preparation, each raw material has good synergism, the preparation made plays nourishing YIN and benefiting blood altogether, effect of heart tonifying and tranquilizing, for insufficiency of heart-YIN, palpitation and amnesia, insomnia and dreamful sleep, the disease such as to be hard and dry.
The manufacture method method of existing raw king's reinforcing the heart preparation is: the Radix Ophiopogonis of getting Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Radix Codonopsis, Poria, Radix Platycodonis and formula ratio 50%, be ground into fine powder; Cinnabaris water flies into impalpable powder, with above-mentioned powder facing-up; Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decocting liquid filters, and filtrate merges, and is condensed into thick paste; All the other medical materials and remaining Radix Ophiopogonis decoct with water secondary, first time 3h, second time 2h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, mixes, pill with above-mentioned fine powder and thick paste, dry, to obtain final product.But inventor finds in research process, adopt in the king's reinforcing the heart preparation made in this way, the effective ingredient of Radix Salviae Miltiorrhizae is not easily measured, and especially salvianolic acid B is not easily measured.Make the clinical efficacy of king's reinforcing the heart preparation desirable not enough.Inventor also finds in technical study, and adopt the inventive method Radix Salviae Miltiorrhizae and Radix Ophiopogonis to be extracted separately, in the king's reinforcing the heart preparation made like this, the effective ingredient of Radix Salviae Miltiorrhizae is easily measured, and the effective ingredient of the Radix Salviae Miltiorrhizae measured is high.This just makes the refine more of this medicine, and drug effect is more remarkable, and clinical efficacy is good.Therefore this technique facilitates the dissolution rate of the dissolution rate of effective ingredient in Radix Salviae Miltiorrhizae, particularly salvianolic acid B, and salvianolic acid B is composition important in Radix Salviae Miltiorrhizae, has very strong pharmacologically active, after the dissolution rate raising of salvianolic acid B, effectively can improve the drug effect of king's reinforcing the heart preparation.
Compared with prior art, in king's reinforcing the heart preparation that the method for the invention makes, the effective ingredient salvianolic acid B in Radix Salviae Miltiorrhizae is easily measured, and the content of danshinolic acid B measured is high.Effectively can improve the drug effect of king's reinforcing the heart preparation.
Invention has been a large amount of experimentatioies, is below the result of experimentation of the present invention:
Experimental example 1: content of danshinolic acid B is investigated
1 instrument and reagent
1.1 tianwangbuxin pills of the present invention, make by the method for embodiment 1.
1.2 existing tianwangbuxin pills: make by following technique:
Prescription: Radix Salviae Miltiorrhizae 25g, Radix Angelicae Sinensis 50g, Rhizoma Acori Graminei 25g, Radix Codonopsis 25g, Poria 25g, Fructus Schisandrae Chinensis 50g, Radix Ophiopogonis 50g, Radix Asparagi 50g, Radix Rehmanniae 200g, Radix Scrophulariae 25g, Radix Polygalae (processed) 25g, Semen Ziziphi Spinosae (parched) 50g, Semen Platycladi 50g, Radix Platycodonis 25g, Radix Glycyrrhizae 25g and Cinnabaris 10g;
Technique: get Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Radix Codonopsis, Poria, Radix Platycodonis and Radix Ophiopogonis 25g, be ground into fine powder; Cinnabaris water flies into impalpable powder, with above-mentioned powder facing-up; Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decocting liquid filters, and filtrate merges, and is condensed into thick paste; All the other medical materials and remaining Radix Ophiopogonis decoct with water secondary, first time 3h, second time 2h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, mixes, pill with above-mentioned fine powder and thick paste, dry, to obtain final product.
1.3 high performance liquid chromatographs: Agilem1100 series; Ultraviolet-uisible spectrophotometer: UV-240IPC type; Balance: Switzerland MEITLERAG-285 (0.01mg); Salvianolic acid B reference substance: Nat'l Pharmaceutical & Biological Products Control Institute provides (for assay); Agents useful for same: water is double distilled water, methanol, acetonitrile are chromatographically pure, and all the other reagent are analytical pure.
2 experimental techniques and result
2.1 chromatographic condition
Chromatographic column: Shim-packCLC-ODS (150mm × 4.6mm); Mobile phase: methanol-acetonitrile-formic acid-water (30:7:l:62); Determined wavelength: 286nm; Column temperature: 30 DEG C; Flow velocity 1ml/min; Sample size: 10 microlitres.With this understanding. salvianolic acid B and other component can reach baseline separation, ask during the reservation of salvianolic acid B in test sample that to contrast brilliant retention time with salvianolic acid B consistent, and negative control is shown in interference: number of theoretical plate: n=7500. separating degree: R=2.0 (R>1.5%), tailing factor: T=0.98 (0.95<T<1.05), repeatability: peak and long-pending RSD=0.79% (n=5, RSD<2.0%).Meet " Chinese Pharmacopoeia } 2005 years-portion measures requirement.Wavelength chooses: and test through UV-2401PC type ultraviolet-uisible spectrophotometer. the maximum absorption wavelength of salvianolic acid B is 286nm, therefore selects 286nm for measuring wavelength.
The preparation of 2.2 reference substance solution
The close salvianolic acid B reference substance 12.14mg taken through phosphorus pentoxide desiccator vacuum drying 24h, adds 50% dissolve with methanol and makes the solution of every 1ml containing 0.1414mg, product solution in contrast.
The preparation of 2.3 need testing solutions
2.3.1 the preparation of need testing solution 1
Get tianwangbuxin pills porphyrize of the present invention, get about 1g, accurately weighed, put in tool plug conical flask, precision adds 50% methanol 25ml, weighed weight, supersound process 30min, lets cool. weighed weight, the weight of less loss is supplied with 50% methanol, shake up, filter, get subsequent filtrate centrifugal (10000rpm) 10min. supernatant as need testing solution 1.
2.3.2 the preparation of need testing solution 2
Enchashment has tianwangbuxin pills porphyrize, get about 1g, accurately weighed, put in tool plug conical flask, precision adds 50% methanol 25ml, weighed weight, supersound process 30min, lets cool. weighed weight, the weight of less loss is supplied with 50% methanol, shake up, filter, get subsequent filtrate centrifugal (10000rpm) 10min. supernatant as need testing solution 2.
The preparation of 2.4 negative control solutions 1, by tianwangbuxin pills prescription proportion of composing of the present invention, gets all the other flavour of a drug except Radix Salviae Miltiorrhizae, by technological requirement, makes not containing the rigidity sample of Radix Salviae Miltiorrhizae, then presses the preparation method of need testing solution 1, make negative control solution 1.
The preparation of negative control solution 2, by existing tianwangbuxin pills prescription proportion of composing, gets all the other flavour of a drug except Radix Salviae Miltiorrhizae, by technological requirement, makes not containing the rigidity sample of Radix Salviae Miltiorrhizae, then presses the preparation method of need testing solution 2, make negative control solution 2.
2.5 blank assay
By above-mentioned condition, draw need testing solution, reference substance solution 1, contrast solution 2, negative control solution 1 and negative control solution 2 appropriate, inject hplc determination respectively, the mensuration of other composition of result to salvianolic acid B is noiseless, meets mensuration requirement.
The preparation of 2.6 standard curves and the investigation of linear relationship
Accurate draw reference substance solution and 3,6,12,18,24,30 microlitres, note people chromatograph of liquid, integrating peak areas value is measured by above-mentioned condition, corner of the eyes integrating peak areas value is vertical coordinate, the amount (microgram) of salvianolic acid B is abscissa, carry out rectilinear regression, obtaining regression equation is: A=1060.19C+1.63, r=0.99998 (n=6).Experiment shows: salvianolic acid B reference substance is good linear relationship with integrating peak areas value in tested amount 0.364 ~ 3.64 microgram range.
2.7 precision test
The same need testing solution 1 that accurate absorption 5 parts is mutually commensurability respectively and need testing solution 2, respectively injection liquid chromatography, measure integrating peak areas value by above-mentioned condition, RSD=0.50% (n=5) and RSD=0.52%.
2.8 stability test
Get the need testing solution 1 and need testing solution 2 got ready respectively, measure immediately, and at room temperature place, measure once every lh, the integrating peak areas value of salvianolic acid B in the need testing solution 1 that result records and need testing solution 2, stable in tested 24h, RSD=0.66% (n=25) and RSD=0.63% (n=25).
2.9 replica test
Get with a collection of tianwangbuxin pills of the present invention and with a collection of existing tianwangbuxin pills each 5 parts respectively, by above-mentioned condition, the content of salvianolic acid B in working sample, the average lucky amount of result is 5.39mg/3g and 3.24mg/3g, RSD=0.51% (n=5) and RSD=0.55% (n=5).
2.10 average recovery is tested
Get tianwangbuxin pills of the present invention and each 5 parts of the existing tianwangbuxin pills of known content respectively, add a certain amount of salvianolic acid B reference substance (0.1214mg/ml × 10ml=1.1214mg) respectively, detection solution is made respectively by the preparation method of need testing solution 1 and need testing solution 2, measure by above-mentioned condition, result average recovery rate be respectively 99.25% and 98.25%, RSD be respectively 1.52% and 1.53%.
2.11 sample determination result
Get tianwangbuxin pills of the present invention and existing tianwangbuxin pills respectively, by the content assaying method drafted, measure the content of salvianolic acid B, the results are shown in Table 1.
The assay result of table 1 salvianolic acid B
As known from Table 1, in tianwangbuxin pills of the present invention, the average content of salvianolic acid B is 5.39mg/3g, and in existing tianwangbuxin pills, the average content of salvianolic acid B is 3.24mg/3g.
Conclusion: compared with existing tianwangbuxin pills, in tianwangbuxin pills of the present invention, the content of the effective ingredient salvianolic acid B of Radix Salviae Miltiorrhizae is higher.In like manner, the preparation of other dosage forms made according to extracting method of the present invention, the active constituent content of Radix Salviae Miltiorrhizae also should increase.
Experimental example 2: Effect tests
1 experiment material
1.1 medicine
1.1.1 tianwangbuxin pills of the present invention, with embodiment 1.
1.1.2 existing tianwangbuxin pills, with experimental example 1.
1.2 animal health ICR Strains of Mouses, body weight 18-20g, male and female half and half, are provided by Beijing Vital River Experimental Animals Technology Co., Ltd..
1.3 instrument XZ-4 mice ambulatory activity count devices (institute of Materia Medica,Chinese Academy of Medical Sciences production).
2 methods
Mice is divided into 4 groups at random, often organizes 10, existing tianwangbuxin pills group, blank group, model group and tianwangbuxin pills group of the present invention.Except blank group, every mice sc only injects Levothyroxinnatrium sodium solution 0.4mg/, simultaneously each group mice ig relative medicine 25mL/kg respectively, model group, blank group ig same volume 0.5%CMC-Na solution.Continuous 6d, every day records Mouse Weight, amount of drinking water, when losing weight appears in model group mice, amount of drinking water increases, after the symptom such as restless, yin-deficient model is set up, and then after last administration, mice is put into mice autonomic activities instrument by Latin square order by lh respectively, mice autonomic activities number of times in record 5min.The results are shown in Table 2.
Table 2 on the impact of mice autonomic activities ( n=10)
Note: compare with blank group, #p<0.01;
Compare with model group, *p<0.05, *p<0.01;
Compare with existing tianwangbuxin pills group +p<0.05.
As seen from table, compare with model group, tianwangbuxin pills group of the present invention, existing tianwangbuxin pills group and blank group ambulatory activity count reduce, therefore tianwangbuxin pills of the present invention and the autonomic activities of existing tianwangbuxin pills to yin vacuity animals all have inhibition, thus play the effect of tranquillizing and allaying excitement, and the effect of tianwangbuxin pills of the present invention is better than existing tianwangbuxin pills.
Detailed description of the invention:
Embodiment 1:
Formula: Radix Salviae Miltiorrhizae 25g, Radix Angelicae Sinensis 50g, Rhizoma Acori Graminei 25g, Radix Codonopsis 25g, Poria 25g, Fructus Schisandrae Chinensis 50g, Radix Ophiopogonis 50g, Radix Asparagi 50g, Radix Rehmanniae 200g, Radix Scrophulariae 25g, Radix Polygalae (processed) 25g, Semen Ziziphi Spinosae (parched) 50g, Semen Platycladi 50g, Radix Platycodonis 25g, Radix Glycyrrhizae 25g, Cinnabaris 10g and adjuvant.
Technique: get Radix Salviae Miltiorrhizae, add 10 times amount soak by water 3 times, each 2 hours, collecting decoction, filter, when to be concentrated into volume every milliliter be 1:1 with the ratio of medical material amount every gram, filter, supernatant adds ethanol, alcohol content is made to reach 70%, pH8 ~ 9 are adjusted with 10% sodium hydroxide solution, cold preservation, cold preservation 36 hours, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1:1, pH5-6 is adjusted with 10% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, and second time 2h, decocting liquid filters, and filtrate is condensed into thick paste, and mix with above-mentioned facing-up powder and thick paste, pill, obtains pill.
Usage and dosage: oral, 8 balls, 3 times on the one.
Specification: every 8 balls are equivalent to decoction pieces 3g.
Embodiment 2:
Formula: Radix Salviae Miltiorrhizae 25g, Radix Angelicae Sinensis 50g, Rhizoma Acori Graminei 25g, Radix Codonopsis 25g, Poria 25g, Fructus Schisandrae Chinensis 50g, Radix Ophiopogonis 50g, Radix Asparagi 50g, Radix Rehmanniae 200g, Radix Scrophulariae 25g, Radix Polygalae (processed) 25g, Semen Ziziphi Spinosae (parched) 50g, Semen Platycladi 50g, Radix Platycodonis 25g, Radix Glycyrrhizae 25g, Cinnabaris 10g and adjuvant.
Technique: get Radix Salviae Miltiorrhizae, add 10 times amount soak by water 3 times, each 2 hours, collecting decoction, filter, when to be concentrated into volume every milliliter be 1:1 with the ratio of medical material amount every gram, filter, supernatant adds ethanol, alcohol content is made to reach 70%, pH8 ~ 9 are adjusted with 10% sodium hydroxide solution, cold preservation, cold preservation 36 hours, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1:1, pH5-6 is adjusted with 10% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, second time 2h, decocting liquid filters, and filtrate is condensed into thick paste, mixes with above-mentioned facing-up powder and thick paste, add the starch mixing of the amount of making 10-20% again, tabletting, dry, obtain tablet.
Usage and dosage: oral, a 2-3 sheet, 2-3 time on the one.
Specification: 0.5g/ sheet.
Embodiment 3:
Formula: Radix Salviae Miltiorrhizae 25g, Radix Angelicae Sinensis 50g, Rhizoma Acori Graminei 25g, Radix Codonopsis 25g, Poria 25g, Fructus Schisandrae Chinensis 50g, Radix Ophiopogonis 50g, Radix Asparagi 50g, Radix Rehmanniae 200g, Radix Scrophulariae 25g, Radix Polygalae (processed) 25g, Semen Ziziphi Spinosae (parched) 50g, Semen Platycladi 50g, Radix Platycodonis 25g, Radix Glycyrrhizae 25g, Cinnabaris 10g and adjuvant.
Technique: get Radix Salviae Miltiorrhizae, add 10 times amount soak by water 3 times, each 2 hours, collecting decoction, filter, when to be concentrated into volume every milliliter be 1:1 with the ratio of medical material amount every gram, filter, supernatant adds ethanol, alcohol content is made to reach 70%, pH8 ~ 9 are adjusted with 10% sodium hydroxide solution, cold preservation, cold preservation 36 hours, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1:1, pH5-6 is adjusted with 10% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, second time 2h, decocting liquid filters, and filtrate is condensed into thick paste, mix with above-mentioned facing-up powder and thick paste, add the starch mixing of the amount of making 10-20% again, granulate, dry, incapsulate, obtain capsule.
Usage and dosage: oral, a 2-3 grain, every day 2-3 time.
Specification: 0.5g/ grain.

Claims (9)

1. the manufacture method of king's reinforcing the heart preparation, it is characterized in that: described king's reinforcing the heart preparation calculates by weight, primarily of Radix Salviae Miltiorrhizae 20-30 part, Radix Angelicae Sinensis 40-60 part, Rhizoma Acori Graminei 20-30 part, Radix Codonopsis 20-30 part, Poria 20-30 part, Fructus Schisandrae Chinensis 40-60 part, Radix Ophiopogonis 40-60 part, Radix Asparagi 40-60 part, Radix Rehmanniae 150-250 part, Radix Scrophulariae 20-30 part, Radix Polygalae (processed) 20-30 part, Semen Ziziphi Spinosae (parched) 40-60 part, Semen Platycladi 40-60 part, Radix Platycodonis 20-30 part, Radix Glycyrrhizae 20-30 part and Cinnabaris 5-15 part makes; The manufacture method of described king's reinforcing the heart preparation is: get Radix Salviae Miltiorrhizae, decoct with water, and decocting liquid filters, and is condensed into concentrated solution, filter, supernatant adds ethanol, makes alcohol content reach 60-85%, adjust pH7 ~ 10 with sodium hydroxide solution, cold preservation, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1-3:3-1, adjusts pH5 ~ 7 with hydrochloric acid solution, filter, be condensed into extractum, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use; Get Radix Ophiopogonis, decoct with water, filter, filtrate reduced in volume, to clear paste, adds ethanol and makes alcohol content reach 55%-85%, stir, and leaves standstill 12-48 hour, and filter, decompression filtrate recycling ethanol is also condensed into extractum, dry, pulverize, obtains Radix Ophiopogonis powder, for subsequent use; Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use; Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, obtains Radix Rehmanniae thick paste, for subsequent use; Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, and second time 2h, decocting liquid filters, and filtrate is condensed into thick paste, mixes, add adjuvant, make pharmaceutical preparation with above-mentioned facing-up powder and Radix Rehmanniae thick paste.
2. the manufacture method of king's reinforcing the heart preparation as claimed in claim 1, it is characterized in that: described king's reinforcing the heart preparation calculates by weight, make primarily of Radix Salviae Miltiorrhizae 25 parts, Radix Angelicae Sinensis 50 parts, Rhizoma Acori Graminei 25 parts, Radix Codonopsis 25 parts, 25 parts, Poria, Fructus Schisandrae Chinensis 50 parts, Radix Ophiopogonis 50 parts, Radix Asparagi 50 parts, Radix Rehmanniae 200 parts, Radix Scrophulariae 25 parts, Radix Polygalae (processed) 25 parts, Semen Ziziphi Spinosae (parched) 50 parts, Semen Platycladi 50 parts, Radix Platycodonis 25 parts, 25 parts, Radix Glycyrrhizae and 10 parts, Cinnabaris, the manufacture method of described king's reinforcing the heart preparation is: get Radix Salviae Miltiorrhizae, add 8-15 times amount soak by water 1-4 time, each 0.5-3 hour, collecting decoction, filter, being concentrated into volume every milliliter with the ratio of medical material amount every gram is 1-3:3-1, filter, supernatant adds ethanol, alcohol content is made to reach 60-85%, pH7 ~ 10 are adjusted with 5-20% sodium hydroxide solution, cold preservation, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1-3:3-1, pH5 ~ 7 are adjusted with 5-20% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 7-12 times of soak by water 2-4 time, each 0.5-3 hour, filter, merging filtrate, concentrating under reduced pressure becomes clear paste, adding ethanol makes alcohol content reach 55%-85%, stirs, and leaves standstill 12-48 hour, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, and second time 2h, decocting liquid filters, and filtrate is condensed into thick paste, mixes, add adjuvant, make pharmaceutical preparation with above-mentioned facing-up powder and thick paste.
3. the manufacture method of king's reinforcing the heart preparation as claimed in claim 2, it is characterized in that: get Radix Salviae Miltiorrhizae, add 10 times amount soak by water 3 times, each 2 hours, collecting decoction, filter, when to be concentrated into volume every milliliter be 1:1 with the ratio of medical material amount every gram, filter, supernatant adds ethanol, alcohol content is made to reach 70%, pH8 ~ 9 are adjusted with 10% sodium hydroxide solution, cold preservation, cold preservation 36 hours, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1:1, pH5-6 is adjusted with 10% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, and second time 2h, decocting liquid filters, and filtrate is condensed into thick paste, mixes, add adjuvant, make pharmaceutical preparation with above-mentioned facing-up powder and thick paste.
4. the manufacture method of the king's reinforcing the heart preparation according to any one of claim 1-3, is characterized in that: described pharmaceutical preparation is oral formulations.
5. the manufacture method of king's reinforcing the heart preparation as claimed in claim 4, is characterized in that: described oral formulations is pill, tablet, capsule or granule.
6. the manufacture method of king's reinforcing the heart preparation as claimed in claim 5, it is characterized in that: described pill makes like this: get Radix Salviae Miltiorrhizae, add 10 times amount soak by water 3 times, each 2 hours, collecting decoction, filter, when to be concentrated into volume every milliliter be 1:1 with the ratio of medical material amount every gram, filter, supernatant adds ethanol, alcohol content is made to reach 70%, pH8 ~ 9 are adjusted with 10% sodium hydroxide solution, cold preservation, cold preservation 36 hours, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1:1, pH5-6 is adjusted with 10% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, and second time 2h, decocting liquid filters, and filtrate is condensed into thick paste, and mix with above-mentioned facing-up powder and thick paste, pill, to obtain final product.
7. the manufacture method of king's reinforcing the heart preparation as claimed in claim 5, it is characterized in that: described tablet makes like this: get Radix Salviae Miltiorrhizae, add 10 times amount soak by water 3 times, each 2 hours, collecting decoction, filter, when to be concentrated into volume every milliliter be 1:1 with the ratio of medical material amount every gram, filter, supernatant adds ethanol, alcohol content is made to reach 70%, pH8 ~ 9 are adjusted with 10% sodium hydroxide solution, cold preservation, cold preservation 36 hours, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1:1, pH5-6 is adjusted with 10% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, second time 2h, and decocting liquid filters, filtrate is condensed into thick paste, mixes with above-mentioned facing-up powder and thick paste, then adds the starch mixing of the amount of making 10-20%, tabletting, dry, to obtain final product.
8. the manufacture method of king's reinforcing the heart preparation as claimed in claim 5, it is characterized in that: described capsule makes like this: get Radix Salviae Miltiorrhizae, add 10 times amount soak by water 3 times, each 2 hours, collecting decoction, filter, when to be concentrated into volume every milliliter be 1:1 with the ratio of medical material amount every gram, filter, supernatant adds ethanol, alcohol content is made to reach 70%, pH8 ~ 9 are adjusted with 10% sodium hydroxide solution, cold preservation, cold preservation 36 hours, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1:1, pH5-6 is adjusted with 10% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, second time 2h, decocting liquid filters, and filtrate is condensed into thick paste, mix with above-mentioned facing-up powder and thick paste, add the starch mixing of the amount of making 10-20% again, granulate, dry, incapsulate, to obtain final product.
9. the manufacture method of king's reinforcing the heart preparation as claimed in claim 5, it is characterized in that: described granule makes like this: get Radix Salviae Miltiorrhizae, add 10 times amount soak by water 3 times, each 2 hours, collecting decoction, filter, when to be concentrated into volume every milliliter be 1:1 with the ratio of medical material amount every gram, filter, supernatant adds ethanol, alcohol content is made to reach 70%, pH8 ~ 9 are adjusted with 10% sodium hydroxide solution, cold preservation, cold preservation 36 hours, filter, reclaim ethanol, adding water to volume every milliliter with the ratio of medical material amount every gram is 1:1, pH5-6 is adjusted with 10% hydrochloric acid solution, filter, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, vacuum drying, be ground into fine powder, obtain Danshen Root, for subsequent use, get Radix Ophiopogonis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Ophiopogonis powder, for subsequent use, Radix Angelicae Sinensis, Radix Codonopsis, Poria and Radix Platycodonis, be ground into fine powder, Cinnabaris water fly into after fine powder with Danshen Root, Radix Ophiopogonis powder and above-mentioned fine powder mixing facing-up, obtain facing-up powder, for subsequent use, Radix Rehmanniae decocts with water three times, first time 3h, second time 2h, third time 1h, and decoction liquor filters, and filtrate merges, and is condensed into thick paste, Fructus Schisandrae Chinensis, Rhizoma Acori Graminei, Radix Scrophulariae, Radix Polygalae (processed), Semen Ziziphi Spinosae (parched), Semen Platycladi and Radix Glycyrrhizae decoct with water 2 times, first time 3h, second time 2h, and decocting liquid filters, filtrate is condensed into thick paste, mixes with above-mentioned facing-up powder and thick paste, then adds the starch mixing of the amount of making 10-20%, granulate, dry, to obtain final product.
CN201510988804.1A 2015-12-25 2015-12-25 Method for preparing Tianwang preparation capable of tonifying heart Withdrawn CN105535428A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107349348A (en) * 2017-06-20 2017-11-17 重庆壹通堂健康管理咨询有限公司 A kind of Chinese medicine preparation of bushing
CN107648424A (en) * 2017-11-07 2018-02-02 贵州拜特制药有限公司 A kind of preparation method of Lycium-rehmannia preparation
CN112807398A (en) * 2021-01-21 2021-05-18 中国人民解放军军事科学院军事医学研究院 Traditional Chinese medicine composition with effects of nourishing yin, clearing heat, nourishing blood and soothing nerves
CN114487182A (en) * 2022-01-20 2022-05-13 广西壮族自治区食品药品检验所 Content determination method of Tianwang heart tonifying preparation

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107349348A (en) * 2017-06-20 2017-11-17 重庆壹通堂健康管理咨询有限公司 A kind of Chinese medicine preparation of bushing
CN107648424A (en) * 2017-11-07 2018-02-02 贵州拜特制药有限公司 A kind of preparation method of Lycium-rehmannia preparation
CN112807398A (en) * 2021-01-21 2021-05-18 中国人民解放军军事科学院军事医学研究院 Traditional Chinese medicine composition with effects of nourishing yin, clearing heat, nourishing blood and soothing nerves
CN114487182A (en) * 2022-01-20 2022-05-13 广西壮族自治区食品药品检验所 Content determination method of Tianwang heart tonifying preparation

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