CN105535780A - Method for preparing common vladimiria root and fructus amomi and six-monarch drug preparation - Google Patents
Method for preparing common vladimiria root and fructus amomi and six-monarch drug preparation Download PDFInfo
- Publication number
- CN105535780A CN105535780A CN201510989314.3A CN201510989314A CN105535780A CN 105535780 A CN105535780 A CN 105535780A CN 201510989314 A CN201510989314 A CN 201510989314A CN 105535780 A CN105535780 A CN 105535780A
- Authority
- CN
- China
- Prior art keywords
- powder
- subsequent use
- filter
- radix
- relative density
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/284—Atractylodes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/285—Aucklandia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
- A61K36/344—Codonopsis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
- A61K36/8888—Pinellia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9064—Amomum, e.g. round cardamom
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
- A61K9/2059—Starch, including chemically or physically modified derivatives; Amylose; Amylopectin; Dextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Alternative & Traditional Medicine (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a method for preparing a common vladimiria root and fructus amomi and six-monarch drug preparation. The common vladimiria root and fructus amomi and six-monarch drug preparation is mainly prepared from common vladimiria root, fructus amomi, radix codonopsis, fried rhizoma atractylodis macrocephalae, poria cocos, radix glycyrrhizae preparata, tangerine peel and ginger processed pinellia. Through the method disclosed by the invention, according to properties of all drugs in a prescription of the common vladimiria root and fructus amomi and six-monarch drug preparation, and functions of the drugs in the formula, a specific process for preparing the common vladimiria root and fructus amomi and six-monarch drug preparation and parameters are improved, so that effective component costunolide of common vladimiria root can be easily measured, and the content of measured costunolide is high, therefore the preparation is more refined, has a more obvious drug effect and has a good clinical effect.
Description
Technical field
The invention belongs to the field of Chinese medicines, be specifically related to a kind of manufacture method of XIANGSHALIUJUNZI preparation.
Background technology
Chinese medicine extraction is a kind of method conventional in herbal pharmaceutical, and chemical composition of Chinese materia medica and complexity thereof, need extract the effective active ingredient of Chinese herbs of human body from the mixture of complexity.To improve drug effect.
XIANGSHALIUJUNZI preparation is made primarily of Radix Codonopsis, Fructus Aurantii Immaturus (parched), the Radix Aucklandiae, Fructus Hordei Germinatus (parched) and Fructus Crataegi (parched); There is replenishing QI to invigorate the spleen, effect of stomach function regulating.For the insufficiency of the spleen stagnation of QI, dyspepsia, distension and fullness in the abdomen, the diseases such as diarrhea with loose stool.
In prior art, XIANGSHALIUJUNZI preparation makes like this: get the Radix Aucklandiae, Fructus Amomi, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) and pulverize, sieve, mixing, separately get Rhizoma Zingiberis Recens, Fructus Jujubae, gradation decocts with water, and filters, get above-mentioned powder, with the general ball of decocting liquid, cold drying, to obtain final product.But inventor finds in research process, adopt existing extraction process to make in XIANGSHALIUJUNZI preparation, the effective ingredient costunolide of the Radix Aucklandiae is not easily measured, and makes the clinical efficacy of XIANGSHALIUJUNZI preparation desirable not enough.
Summary of the invention
The invention provides a kind of manufacture method of XIANGSHALIUJUNZI preparation.The present invention is according to the character of medicine each in XIANGSHALIUJUNZI preparation recipe, and this medicine role in prescription, the concrete processing technology of XIANGSHALIUJUNZI preparation and parameter are improved, the effective ingredient costunolide of the Radix Aucklandiae is easily measured, and the costunolide amount measured is high, make the refine more of this medicine, drug effect is more remarkable, and clinical efficacy is good.
To achieve these goals, the invention provides following technical scheme: a kind of manufacture method of XIANGSHALIUJUNZI preparation, described XIANGSHALIUJUNZI preparation calculates by weight, makes primarily of Radix Aucklandiae 60-80 part, Fructus Amomi 70-90 part, Radix Codonopsis 90-110 part, Rhizoma Atractylodis Macrocephalae (parched) 190-210 part, Poria 190-210 part, Radix Glycyrrhizae Preparata 60-80 part, Pericarpium Citri Reticulatae 70-90 part and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) 90-110 part; The manufacture method of described XIANGSHALIUJUNZI preparation is: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues extracting in water, merges Aqueous extracts, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 55-65% again, reflux, extract, 2-3 time, filters, merging filtrate, and reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and are condensed into extractum, dry, pulverize, and obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, decoct with water, filter, filtrate reduced in volume, to clear paste, adds ethanol and makes alcohol content reach 55%-85%, stir, and leaves standstill 12-48 hour, and filter, decompression filtrate recycling ethanol is also condensed into extractum, dry, pulverize, and obtains Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get Rhizoma Zingiberis Recens and Fructus Jujubae, gradation decocts with water, and filter, decocting liquid mixes with above-mentioned volatile oil, powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, adds adjuvant, makes pharmaceutical preparation.
In the manufacture method of aforesaid XIANGSHALIUJUNZI preparation, described XIANGSHALIUJUNZI preparation calculates by weight, makes primarily of the Radix Aucklandiae 70 parts, Fructus Amomi 80 parts, Radix Codonopsis 100 parts, Rhizoma Atractylodis Macrocephalae (parched) 200 parts, 200 parts, Poria, Radix Glycyrrhizae Preparata 70 parts, Pericarpium Citri Reticulatae 80 parts and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) 100 parts; The manufacture method of described XIANGSHALIUJUNZI preparation is: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues add water extraction 1-3 time of 8-12 times amount, each 1-3h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 6-10 times amount 55-65% again, reflux, extract, 2-3 time, each 0.5-3h, filter, merging filtrate, reclaims ethanol, obtains alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 7-12 times of soak by water 2-4 time, each 0.5-3 hour, filter, merging filtrate, concentrating under reduced pressure becomes clear paste, adding ethanol makes alcohol content reach 55%-85%, stirs, and leaves standstill 12-48 hour, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get Rhizoma Zingiberis Recens and Fructus Jujubae, gradation decocts with water, and filter, decocting liquid mixes with above-mentioned volatile oil, powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, adds adjuvant, makes pharmaceutical preparation.
In the manufacture method of aforesaid XIANGSHALIUJUNZI preparation, get the Radix Aucklandiae, use water vapour distillation volatile oil, for subsequent use; Medicinal residues add the water extraction 2 times of 10 times amount, each 2h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 8 times amount 60% again, reflux, extract, 3 times, each 1h, filters, merging filtrate, reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get Rhizoma Zingiberis Recens and Fructus Jujubae, gradation decocts with water, and filter, decocting liquid mixes with above-mentioned volatile oil, powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, adds adjuvant, makes pharmaceutical preparation.
In the manufacture method of aforesaid XIANGSHALIUJUNZI preparation, described pharmaceutical preparation is oral formulations.
In the manufacture method of aforesaid XIANGSHALIUJUNZI preparation, described oral formulations is pill, tablet, capsule or granule.
In the manufacture method of aforesaid XIANGSHALIUJUNZI preparation, described pill makes like this: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues add the water extraction 2 times of 10 times amount, each 2h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 8 times amount 60% again, reflux, extract, 3 times, each 1h, filters, merging filtrate, reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get the amount of making 10-25% Rhizoma Zingiberis Recens and Fructus Jujubae, gradation decocts with water, and filters, and get the mixing of above-mentioned powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, with the general ball of decocting liquid, cold drying, to obtain final product.
In the manufacture method of aforesaid XIANGSHALIUJUNZI preparation, described tablet makes like this: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues add the water extraction 2 times of 10 times amount, each 2h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 8 times amount 60% again, reflux, extract, 3 times, each 1h, filters, merging filtrate, reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get the amount of making 10-25% Rhizoma Zingiberis Recens and Fructus Jujubae, gradation decocts with water, and filters, gets above-mentioned powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, mix with decocting liquid, add starch and the sucrose of the amount of making 10-20%, tabletting, dry, to obtain final product.
In the manufacture method of aforesaid XIANGSHALIUJUNZI preparation, described capsule makes like this: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues add the water extraction 2 times of 10 times amount, each 2h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 8 times amount 60% again, reflux, extract, 3 times, each 1h, filters, merging filtrate, reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get the amount of making 10-25% Rhizoma Zingiberis Recens and Fructus Jujubae, gradation decocts with water, and filters, gets above-mentioned powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, mix with decocting liquid, add starch and the magnesium stearate of the amount of making 10-20%, and granulate, cold drying, incapsulates, and to obtain final product.
In the manufacture method of aforesaid XIANGSHALIUJUNZI preparation, described granule makes like this: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues add the water extraction 2 times of 10 times amount, each 2h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 8 times amount 60% again, reflux, extract, 3 times, each 1h, filters, merging filtrate, reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get the amount of making 10-25% Rhizoma Zingiberis Recens and Fructus Jujubae, gradation decocts with water, and filters, gets above-mentioned powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, mix with decocting liquid, add starch and the magnesium stearate of the amount of making 10-20%, and granulate, cold drying, to obtain final product.
The present invention adopts the Radix Aucklandiae, Fructus Amomi, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) to make into medicine, i.e. XIANGSHALIUJUNZI preparation, and each raw material has good synergism, and the preparation made has replenishing QI to invigorate the spleen, effect of stomach function regulating.For the insufficiency of the spleen stagnation of QI, dyspepsia, distension and fullness in the abdomen, the diseases such as diarrhea with loose stool.
The manufacture method method of existing XIANGSHALIUJUNZI preparation is: get the Radix Aucklandiae, Fructus Amomi, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) and pulverize, sieve, mixing, separately gets Rhizoma Zingiberis Recens, Fructus Jujubae, gradation decocts with water, and filters, gets above-mentioned powder, with the general ball of decocting liquid, cold drying, to obtain final product.But inventor finds in research process, adopt in the XIANGSHALIUJUNZI preparation made in this way, the effective ingredient of the Radix Aucklandiae is not easily measured.Make the clinical efficacy of XIANGSHALIUJUNZI preparation desirable not enough.Inventor also finds in technical study, and adopt the inventive method the Radix Aucklandiae and Radix Codonopsis to be extracted separately, in the XIANGSHALIUJUNZI preparation made like this, the effective ingredient of the Radix Aucklandiae is easily measured, and the effective ingredient of the Radix Aucklandiae measured is high.This just makes the refine more of this medicine, and drug effect is more remarkable, and clinical efficacy is good.Therefore this technique facilitates the dissolution rate of effective ingredient in the Radix Aucklandiae, the particularly dissolution rate of costunolide, costunolide is composition important in the Radix Aucklandiae, has very strong pharmacologically active, after the dissolution rate raising of costunolide, effectively can improve the drug effect of XIANGSHALIUJUNZI preparation.
Compared with prior art, in the XIANGSHALIUJUNZI preparation that the method for the invention makes, the effective ingredient costunolide in the Radix Aucklandiae is easily measured, and the content of the costunolide measured is high.Effectively can improve the drug effect of XIANGSHALIUJUNZI preparation.
Invention has been a large amount of experimentatioies, is below the result of experimentation of the present invention:
Experimental example 1: content determination of costunolide is investigated
1 instrument and reagent
1.1 XIANGSHA LIUJU WAN of the present invention, make by the method for embodiment 1.
1.2 existing XIANGSHA LIUJU WAN: make by following technique:
Prescription: Radix Aucklandiae 70g, Fructus Amomi 80g, Radix Codonopsis 100g, Rhizoma Atractylodis Macrocephalae (parched) 200g, Poria 200g, Radix Glycyrrhizae Preparata 70g, Pericarpium Citri Reticulatae 80g and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) 100g.
Technique: get the Radix Aucklandiae, Fructus Amomi, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) and pulverize, sieve, mixing, separately get Rhizoma Zingiberis Recens 10g, Fructus Jujubae 20g, gradation decocts with water, and filter, get above-mentioned powder, with the general ball of decocting liquid, cold drying, to obtain final product.
1.32695 type high performance liquid chromatography (U.S., Waters company); KromasilC18 post (250mm × 4.6mm, 5 μm).Costunolide reference substance is purchased from Rui Qi bio tech ltd, Shanghai, and through fusing point, 1HNMR, 13CNMR confirmation, HPLC areas of peak normalization method measures its content and reaches more than 98%; Methanol is chromatographically pure; Water is distilled water; Other reagent are analytical pure.
2 experimental techniques and result
2.1 chromatographic condition
Chromatographic column is KromasilC18 (250mm × 4.6mm, 5 μm); Mobile phase: methanol-water (60: 40); Determined wavelength: 225nm; Flow velocity is 1.0mL/min; Column temperature: 30 DEG C, sampling volume 10 μ L.
The preparation of 2.2 solution
2.2.1 10mg costunolide reference substance is got in the preparation of reference substance solution, accurately weighed, puts in 25mL measuring bottle, by methanol constant volume, obtains reference substance solution.
2.2.2 2g XIANGSHA LIUJU WAN of the present invention is got in the preparation of need testing solution 1, and precision measures 0.1g, and put in tool plug conical flask, precision adds methanol to 25mL, shakes up, and filters, obtains need testing solution 1.
2.2.3 the existing XIANGSHA LIUJU WAN of 2g is got in the preparation of need testing solution 2, and precision measures 0.1g, and put in tool plug conical flask, precision adds methanol to 25mL, shakes up, and filters, obtains need testing solution 2.
2.3 linear relationships are investigated
Precision takes 1.05mg costunolide reference substance, puts in 25mL measuring bottle, with dissolve with methanol and standardize solution, obtains the reference substance stock solution of 0.042mg/mL; Accurate draw this solution 0.25,0.5,1.0,2.5,5mL, be placed in 10mL measuring bottle respectively, add methanol dilution to scale, shake up, concentration is followed successively by 1.05 μ g/mL, 2.10 μ g/mL, 4.20 μ g/mL, 10.50 μ g/mL, 21.00 μ g/mL.Accurate absorption 10 μ L injection liquid chromatographies respectively, measure peak area value.Carry out linear regression with absolute sample size to peak area, obtaining regression equation is: A=2.037 × 107W-1.265 × 105 (r=0.9999), and the costunolide range of linearity is respectively 1.05 ~ 21.00 μ g/mL.
2.4 precision test
Accurate absorption 10 μ L reference substance solution, repeat sample introduction 6 times.The RSD recording costunolide area is 1.30%.Show that system precision is good.
2.5 stability test
Get need testing solution 1 and each 10 μ l of need testing solution 2 respectively, respectively at 0h, 2h, 4h, 6h, 12h sample introduction, calculate, measure integrating peak areas value by above-mentioned chromatographic condition.Result RSD=0.91%, RSD=0.95%, show that need testing solution is stable in 12h.
2.6 repeated experiment
Precision takes XIANGSHA LIUJU WAN of the present invention and each 6 parts of existing XIANGSHA LIUJU WAN respectively, according to legal system available test sample solution 1 and need testing solution 2 below " 2.2 " item, by above-mentioned chromatographic condition sample introduction, measures.Result RSD=1.05% and RSD=1.08%, shows that this method repeatability is good.
The serviceability test of 2.7 chromatographs
Get same need testing solution 1 and need testing solution 2 respectively, " 2.1 " under chromatographic condition, parallel assay content, RSD=1.7% and RSD=1.72% of content.The chromatograph good tolerance of the method is described.
2.8 recovery tests get XIANGSHA LIUJU WAN of the present invention and each 6 parts of the existing XIANGSHA LIUJU WAN of known content respectively, every part of about 1.5g, precise weighing, precision adds a certain amount of costunolide reference substance respectively, prepare need testing solution as stated above, by above-mentioned chromatographic condition sample introduction 10 μ l, measure, calculate the response rate.Result average recovery rate is 101.08% and 99.55%, RSD=0.99% and RSD=0.10%, shows that the method response rate is good.
2.6 sample sizes measure
Get XIANGSHA LIUJU WAN of the present invention and each 6 parts of existing XIANGSHA LIUJU WAN respectively, porphyrize, get 3g, accurately weighed, every part press respectively need testing solution 1 and need testing solution 2 method preparation after, precision takes need testing solution 1 and need testing solution each 1, every part of each 10 μ l, measures content determination of costunolide in XIANGSHA LIUJU WAN of the present invention and existing XIANGSHA LIUJU WAN in accordance with the law.The results are shown in Table 1.
The assay result of table 1 costunolide
As known from Table 1, in XIANGSHA LIUJU WAN of the present invention, the average content of costunolide is 0.22%, and in existing XIANGSHA LIUJU WAN, the average content of costunolide is 0.12%.
Conclusion: compared with existing XIANGSHA LIUJU WAN, in XIANGSHA LIUJU WAN of the present invention, the content of the effective ingredient costunolide of the Radix Aucklandiae is higher.In like manner, the preparation of other dosage forms made according to extracting method of the present invention, the active constituent content of the Radix Aucklandiae also should increase.
Experimental example 2: Effect tests
1 experiment material
1.1 medicine
1.1.1 XIANGSHA LIUJU WAN of the present invention, with embodiment 1.
1.1.2 existing XIANGSHA LIUJU WAN, with experimental example 1.
1..2 reagent neostigmine methylsulfate injection (1mg/2mL.1), Shanghai Xinyi Pharmaceutical Factory is produced; Atropine sulfate injection 10mg/mL, pharmaceutical factory, Tianmen, Hubei produces; Histamine phosphate, Shanghai Inst. of Biochemistry, Chinese Academy of Sciences's subpackage.
1.3 animal Kunming mouses, SPF level, body weight 18-20g, male and female half and half.Cavia porcellus, body weight 280-300g, male and female, are provided by Hubei Province's medical experiment animal center, the quality certification number, dynamic No. 19-068th, the word of doctor.
1.4 statistical method
Measured value is used
represent, carry out statistical procedures with paired t-test.
2 methods
2.1 intestinal propulsion operation tests
Get Kunming mouse 30, body weight 18-20g, male and female half and half, by mice sex, body weight sequence is divided into 3 groups at random.XIANGSHA LIUJU WAN group of the present invention (gives XIANGSHA LIUJU WAN of the present invention, dosage 10g/kg), existing XIANGSHA LIUJU WAN group (gives existing XIANGSHA LIUJU WAN, dosage 10g/kg), negative control group (giving water for injection 20mL/kg).
By Mouse Weight 10gig administration 0.2mL, successive administration 5d.Experiment mice is fasting 24h before last administration.After last administration, the every Mus ig of 1h gives 15% active carbon arabic gum suspension 0.2mL, and after 20min, mice is put to death in dislocation, cuts open the belly, measures activated carbon displacement, and then measure total small intestinal length from pylorus, calculates ink propulsive rate.Experimental result is in table 2.
Table 2 respectively group mouse small intestine ahead running impact (
n=10)
Note: compare with negative control group,
*p<0.05,
*p<0.01;
Compare with existing XIANGSHA LIUJU WAN group
+p<0.05.
Experimental result shows, XIANGSHA LIUJU WAN of the present invention and existing XIANGSHA LIUJU WAN obviously can suppress the ahead running of mouse small intestine, more all have significant difference with negative control group.And XIANGSHA LIUJU WAN effect of the present invention is better than existing XIANGSHA LIUJU WAN.
2.2 impacts emptying on Mouse Stomach
Get Kunming mouse 50, body weight 18 ~ 20g, male and female half and half.Experiment point combo, route of administration and administration time are with 2.1.
Fasting 24h before last administration, last administration is ip (getting supernatant after tested material is centrifugal), 40min after last administration, every Mus gavage gives 0.2mL0.1% methylol solution, after 20min, mice is put to death in dislocation, cuts open the belly and wins stomach and be placed in small beaker, add 10mL distilled water, gastric content is fully washed in distilled water, uses NaHCO
3, solution adjust ph to 6.0 ~ 6.5, centrifugal 10min, gets supernatant, measures optical density (OD), by above-listed various calculating methylol Stomach residue rate.Experimental result is in table 3.
Table 3 on the emptying impact of Mouse Stomach (
n=10)
Note: compare with negative control group,
*p<0.05,
*p<0.01;
Compare with existing XIANGSHA LIUJU WAN group
+p<0.05.
As seen from table, experimental result shows, XIANGSHA LIUJU WAN of the present invention and existing XIANGSHA LIUJU WAN all can significantly slow down Mouse Stomach emptying.And the effect of XIANGSHA LIUJU WAN of the present invention is better than existing XIANGSHA LIUJU WAN.
Detailed description of the invention:
Embodiment 1:
Formula: Radix Aucklandiae 70g, Fructus Amomi 80g, Radix Codonopsis 100g, Rhizoma Atractylodis Macrocephalae (parched) 200g, Poria 200g, Radix Glycyrrhizae Preparata 70g, Pericarpium Citri Reticulatae 80g and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) 100g and adjuvant.
Technique: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues add the water extraction 2 times of 10 times amount, each 2h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 8 times amount 60% again, reflux, extract, 3 times, each 1h, filters, merging filtrate, reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get Rhizoma Zingiberis Recens 10g, Fructus Jujubae 20g, gradation decocts with water, and filter, get above-mentioned powder, with the general ball of decocting liquid, cold drying, to obtain final product.
Usage and dosage: oral, a 6-9g, every day 2-3 time.
Specification: 3g/ ball.
Embodiment 2:
Formula: Radix Aucklandiae 70g, Fructus Amomi 80g, Radix Codonopsis 100g, Rhizoma Atractylodis Macrocephalae (parched) 200g, Poria 200g, Radix Glycyrrhizae Preparata 70g, Pericarpium Citri Reticulatae 80g and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) 100g and adjuvant.
Technique: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues add the water extraction 2 times of 10 times amount, each 2h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 8 times amount 60% again, reflux, extract, 3 times, each 1h, filters, merging filtrate, reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get Rhizoma Zingiberis Recens 10g, Fructus Jujubae 20g, gradation decocts with water, and filters, gets above-mentioned powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, mix with decocting liquid, add starch and the sucrose of the amount of making 10-20%, tabletting, dry, to obtain final product.
Usage and dosage: oral, a 2-3 sheet, every day 2-3 time.
Specification: 0.5g/ sheet.
Embodiment 3:
Formula: Radix Aucklandiae 70g, Fructus Amomi 80g, Radix Codonopsis 100g, Rhizoma Atractylodis Macrocephalae (parched) 200g, Poria 200g, Radix Glycyrrhizae Preparata 70g, Pericarpium Citri Reticulatae 80g and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) 100g and adjuvant.
Technique: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues add the water extraction 2 times of 10 times amount, each 2h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 8 times amount 60% again, reflux, extract, 3 times, each 1h, filters, merging filtrate, reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get Rhizoma Zingiberis Recens 10g, Fructus Jujubae 20g, gradation decocts with water, and filters, gets above-mentioned powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, mix with decocting liquid, add starch and the magnesium stearate of the amount of making 10-20%, and granulate, cold drying, incapsulates, and obtains capsule.
Usage and dosage: oral, a 2-3 grain, every day 2-3 time.
Specification: 0.5g/ grain.
Claims (9)
1. the manufacture method of an XIANGSHALIUJUNZI preparation, it is characterized in that: described XIANGSHALIUJUNZI preparation calculates by weight, make primarily of Radix Aucklandiae 60-80 part, Fructus Amomi 70-90 part, Radix Codonopsis 90-110 part, Rhizoma Atractylodis Macrocephalae (parched) 190-210 part, Poria 190-210 part, Radix Glycyrrhizae Preparata 60-80 part, Pericarpium Citri Reticulatae 70-90 part and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) 90-110 part; The manufacture method of described XIANGSHALIUJUNZI preparation is: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues extracting in water, merges Aqueous extracts, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 55-65% again, reflux, extract, 2-3 time, filters, merging filtrate, and reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and are condensed into extractum, dry, pulverize, and obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, decoct with water, filter, filtrate reduced in volume, to clear paste, adds ethanol and makes alcohol content reach 55%-85%, stir, and leaves standstill 12-48 hour, and filter, decompression filtrate recycling ethanol is also condensed into extractum, dry, pulverize, and obtains Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get Rhizoma Zingiberis Recens and Fructus Jujubae, gradation decocts with water, and filter, decocting liquid mixes with above-mentioned volatile oil, powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, adds adjuvant, makes pharmaceutical preparation.
2. the manufacture method of XIANGSHALIUJUNZI preparation as claimed in claim 1, it is characterized in that: described XIANGSHALIUJUNZI preparation calculates by weight, make primarily of the Radix Aucklandiae 70 parts, Fructus Amomi 80 parts, Radix Codonopsis 100 parts, Rhizoma Atractylodis Macrocephalae (parched) 200 parts, 200 parts, Poria, Radix Glycyrrhizae Preparata 70 parts, Pericarpium Citri Reticulatae 80 parts and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) 100 parts; The manufacture method of described XIANGSHALIUJUNZI preparation is: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues add water extraction 1-3 time of 8-12 times amount, each 1-3h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 6-10 times amount 55-65% again, reflux, extract, 2-3 time, each 0.5-3h, filter, merging filtrate, reclaims ethanol, obtains alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 7-12 times of soak by water 2-4 time, each 0.5-3 hour, filter, merging filtrate, concentrating under reduced pressure becomes clear paste, adding ethanol makes alcohol content reach 55%-85%, stirs, and leaves standstill 12-48 hour, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get Rhizoma Zingiberis Recens and Fructus Jujubae, gradation decocts with water, and filter, decocting liquid mixes with above-mentioned volatile oil, powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, adds adjuvant, makes pharmaceutical preparation.
3. the manufacture method of XIANGSHALIUJUNZI preparation as claimed in claim 2, is characterized in that: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues add the water extraction 2 times of 10 times amount, each 2h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 8 times amount 60% again, reflux, extract, 3 times, each 1h, filters, merging filtrate, reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get Rhizoma Zingiberis Recens and Fructus Jujubae, gradation decocts with water, and filter, decocting liquid mixes with above-mentioned volatile oil, powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, adds adjuvant, makes pharmaceutical preparation.
4. the manufacture method of the XIANGSHALIUJUNZI preparation as described in claim 1-3, is characterized in that: described pharmaceutical preparation is oral formulations.
5. the manufacture method of XIANGSHALIUJUNZI preparation as claimed in claim 4, is characterized in that: described oral formulations is pill, tablet, capsule or granule.
6. the manufacture method of XIANGSHALIUJUNZI preparation as claimed in claim 5, is characterized in that: described pill makes like this: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues add the water extraction 2 times of 10 times amount, each 2h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 8 times amount 60% again, reflux, extract, 3 times, each 1h, filters, merging filtrate, reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get the amount of making 10-25% Rhizoma Zingiberis Recens and Fructus Jujubae, gradation decocts with water, and filters, and get the mixing of above-mentioned powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, with the general ball of decocting liquid, cold drying, to obtain final product.
7. the manufacture method of XIANGSHALIUJUNZI preparation as claimed in claim 5, is characterized in that: described tablet makes like this: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues add the water extraction 2 times of 10 times amount, each 2h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 8 times amount 60% again, reflux, extract, 3 times, each 1h, filters, merging filtrate, reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get the amount of making 10-25% Rhizoma Zingiberis Recens and Fructus Jujubae, gradation decocts with water, and filters, gets above-mentioned powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, mix with decocting liquid, add starch and the sucrose of the amount of making 10-20%, tabletting, dry, to obtain final product.
8. the manufacture method of XIANGSHALIUJUNZI preparation as claimed in claim 5, is characterized in that: described capsule makes like this: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues add the water extraction 2 times of 10 times amount, each 2h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 8 times amount 60% again, reflux, extract, 3 times, each 1h, filters, merging filtrate, reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get the amount of making 10-25% Rhizoma Zingiberis Recens and Fructus Jujubae, gradation decocts with water, and filters, gets above-mentioned powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, mix with decocting liquid, add starch and the magnesium stearate of the amount of making 10-20%, and granulate, cold drying, incapsulates, and to obtain final product.
9. the manufacture method of XIANGSHALIUJUNZI preparation as claimed in claim 5, is characterized in that: described granule makes like this: get the Radix Aucklandiae, uses water vapour distillation volatile oil, for subsequent use; Medicinal residues add the water extraction 2 times of 10 times amount, each 2h, filter, merging filtrate, after being condensed into concentrated solution, for subsequent use; Filtering residue adds the alcoholic solution of 8 times amount 60% again, reflux, extract, 3 times, each 1h, filters, merging filtrate, reclaim ethanol, obtain alcohol extract, alcohol extract and above-mentioned Aqueous extracts merge, and when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, obtain Radix Aucklandiae dried cream powder, for subsequent use; Get Radix Codonopsis, add 10 times of soak by water 3 times, each 2 hours, filter, merging filtrate, when being evaporated to 50 DEG C, relative density is the extractum of 1.05-1.10, adding ethanol makes alcohol content reach 65%, stirs, and leaves standstill 24 hours, filter, decompression filtrate recycling ethanol to be concentrated into relative density when relative density is 50 DEG C be the extractum of 1.15-1.20, dry, pulverize, obtain Radix Codonopsis powder, for subsequent use; Fructus Amomi, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Glycyrrhizae Preparata, Pericarpium Citri Reticulatae and Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) are ground into fine powder, sieve, mixing; Separately get the amount of making 10-25% Rhizoma Zingiberis Recens and Fructus Jujubae, gradation decocts with water, and filters, gets above-mentioned powder, Radix Codonopsis powder and Radix Aucklandiae dried cream powder, mix with decocting liquid, add starch and the magnesium stearate of the amount of making 10-20%, and granulate, cold drying, to obtain final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510989314.3A CN105535780A (en) | 2015-12-25 | 2015-12-25 | Method for preparing common vladimiria root and fructus amomi and six-monarch drug preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510989314.3A CN105535780A (en) | 2015-12-25 | 2015-12-25 | Method for preparing common vladimiria root and fructus amomi and six-monarch drug preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105535780A true CN105535780A (en) | 2016-05-04 |
Family
ID=55815663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510989314.3A Withdrawn CN105535780A (en) | 2015-12-25 | 2015-12-25 | Method for preparing common vladimiria root and fructus amomi and six-monarch drug preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105535780A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107744577A (en) * | 2016-12-30 | 2018-03-02 | 四川好医生攀西药业有限责任公司 | A kind of Chinese medicine composition for treating mucosal lesion |
| CN108187008A (en) * | 2018-02-08 | 2018-06-22 | 温州市中心医院 | It is a kind of to be used to treat Chinese medicine of gastric cancer and preparation method thereof |
| CN111000959A (en) * | 2019-12-05 | 2020-04-14 | 佛山市禅城区中心医院有限公司 | Chinese patent medicine for treating spleen deficiency syndrome postoperative fatigue syndrome and preparation method thereof |
| CN115554375A (en) * | 2022-11-23 | 2023-01-03 | 昆明中药厂有限公司 | Stomach calming Xiangsha granules with high molding rate, low hygroscopicity and good dissolubility and fluidity |
-
2015
- 2015-12-25 CN CN201510989314.3A patent/CN105535780A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107744577A (en) * | 2016-12-30 | 2018-03-02 | 四川好医生攀西药业有限责任公司 | A kind of Chinese medicine composition for treating mucosal lesion |
| CN107744577B (en) * | 2016-12-30 | 2020-12-22 | 四川好医生攀西药业有限责任公司 | Traditional Chinese medicine composition for treating gastric mucosal injury |
| CN108187008A (en) * | 2018-02-08 | 2018-06-22 | 温州市中心医院 | It is a kind of to be used to treat Chinese medicine of gastric cancer and preparation method thereof |
| CN111000959A (en) * | 2019-12-05 | 2020-04-14 | 佛山市禅城区中心医院有限公司 | Chinese patent medicine for treating spleen deficiency syndrome postoperative fatigue syndrome and preparation method thereof |
| CN115554375A (en) * | 2022-11-23 | 2023-01-03 | 昆明中药厂有限公司 | Stomach calming Xiangsha granules with high molding rate, low hygroscopicity and good dissolubility and fluidity |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101073606B (en) | Method for separating and extracting white Peony Root | |
| CN101513519B (en) | Chinese medicinal composition for invigorating Qi and nourishing blood, preparation method and quality control method thereof | |
| CN106324174A (en) | Quality standard for traditional Chinese medicine formula granules | |
| CN1806846A (en) | Chinese medicinal composition, its preparation process and quality control method | |
| CN102688431A (en) | Kidney-reinforcing oral liquid and preparation method thereof | |
| CN105535780A (en) | Method for preparing common vladimiria root and fructus amomi and six-monarch drug preparation | |
| CN101700289B (en) | Traditional Chinese medicine extract composition for treating gastrointestinal dysfunction or irritable bowel syndrome (IBS), and preparation method and applications thereof in preparation of medicaments | |
| CN106290599B (en) | Content determination method of traditional Chinese medicine composition | |
| CN103784761A (en) | Drug for treatment of vertigo and preparation method thereof | |
| CN102539599B (en) | Method for detecting liver-enhancing medicine | |
| CN102488837A (en) | Sugar-free granule for treating chronic fatigue syndrome and preparation method and detecting method thereof | |
| CN101496870A (en) | Chinese medicinal composition for resolving phlegm and suppressing cough as well as preparation method and quality control method thereof | |
| CN100493579C (en) | Chinese medicine composition, and its preparing method and quality control method | |
| CN105535428A (en) | Method for preparing Tianwang preparation capable of tonifying heart | |
| CN101912594A (en) | Traditional Chinese medicine preparation for treating gastrointestinal diseases | |
| CN101559192B (en) | Traditional Chinese medicine granular formulation for warming stomach and regulating middle warmer | |
| CN108226325A (en) | Roripa montana gives birth to the method for building up of arteries and veins oral liquid composition finger-print | |
| CN102133242A (en) | Tuckahoe extract and preparation method thereof | |
| CN114053337A (en) | Traditional Chinese medicine composition with mental relief and anti-depression effects and preparation method thereof | |
| CN105497713A (en) | Preparation method of inflammation diminishing and cough relieving preparation | |
| CN102008704B (en) | Detection method for composition having middle-warming stomach harmonizing function | |
| CN111562324B (en) | Method for detecting contents of multiple index components of breast-eliminating and stasis-removing capsule | |
| CN103316074A (en) | Medicine composite of halenia corni extract, astragalus extract and liquorice extract as well as preparation and application of medicine composite | |
| CN100518783C (en) | Method of testing oral disintegration tablets of 'Huo Xiang Zheng Qi' | |
| CN101953978A (en) | Heart-soothing and lipid-lowering tablet medicine quality standard detecting method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20160504 |
|
| WW01 | Invention patent application withdrawn after publication | ||
| DD01 | Delivery of document by public notice |
Addressee: Zhao Hai Document name: Review Business Special Letter |
|
| DD01 | Delivery of document by public notice |