A kind of pure natural mosquito-repellent agent
Technical field
The present invention relates to a kind of pure natural mosquito-repellent agent and application thereof, belong to the cosmetics of everyday use field.
Background technology
Mosquito is one of four pests, is again the portable object of virus.Along with the development of society, the human living standard constantly provides raising, and also in continuous increase, various mosquito repellent products arise at the historic moment people in succession to the consciousness of healthy and self-protection.At present, the product of mosquito repellent class is a lot of on the market, mainly concentrates on coiled mosquito-repellent incense, the sheet that kindles mosquito repellent incense, aerosol mosquitocide, floral water, mosquito-dispelling cream, dimethyl carbate, heating mosquito-repellent incense liquid etc.These mosquito repellent product majorities are to rely on agricultural chemicals or chemical analysis to reach repelling effect, and having only minority is to have come mosquito-repelling function with the harmless composition of Nantural non-toxic.And this part product mostly is a liquid, carries inconvenience and is subject to the influence of extraneous factors such as temperature, pressure.In this case, it is very urgent to develop a kind of solid mosquito repellent product of nontoxic safety-type.
At present, people's mosquito repellent articles for use the most commonly used are mosquito-repellent incenses in summer, do not know that but this can damage human body.In fact, the effective ingredient of most of mosquito-repellent incenses is pyrethrin insecticides, and organic filler, binder, dyestuff and other additives etc., therefore contains many harmful materials in the cigarette of mosquito incense burning, might bring out diseases such as asthma.According to measuring and calculating, the particulate that point one volume mosquito-repellent incense is emitted is roughly the same with the amount of burning 100 left and right sides cigarettes, and the ultrafine dust that discharges can enter and be deposited in the lung, possibly cause asthma in a short time, for a long time then maybe cause cancer.Next is the carcinogen that mosquito-repellent incense base material imperfect combustion produces, and some can stimulate the compound of the upper respiratory tract, and these materials can make people's nervous system poison.
Essential oil is to refine the volatile flavor extract a kind of flower from plant, leaf, stem, root or the fruit, and common have Rosa Damascana, Lavender, a tea tree ethereal oil etc.Essential oil not only has the smell of fragrance, plays releive nervous, bracing effect, can also play the effect of mosquito repellent.Yet existing essential oil mosquito repellent series products prescription is unreasonable, and repelling effect is difficult to accomplish to continue, and essential oil product is as liquid form product, and its extremely strong volatility has also been brought inconvenience to use.
Summary of the invention
Technical problem to be solved by this invention is, a kind of culicifuge that contains the natural essential oil composition, preferred is provided, and the invention still further relates to a kind of culicifuge that contains the solid natural derived essential oil.
The alleged technical problem of the present invention is solved by following technical scheme:
A kind of culicifuge, it comprises the essential oil of following volume proportion: lavender 1-3 part, daphne odera 1-3 part, curcuma zedoary 1-3 part, tarragon 1-3 part and camphortree leaf 1-3 part, preferably equal-volume carries out proportioning.
In a preferred implementation of the present invention, also comprise the conventional auxiliary material that uses in the said culicifuge.
In a preferred implementation of the present invention, described culicifuge is spray, tablet, solution.
In a preferred implementation of the present invention, described essential oil is that raw material passes through CO
2Supercritical extract obtains.
In a preferred implementation of the present invention, described culicifuge also comprise this area other have the composition of anophelifuge activity.
In a preferred implementation of the present invention, described essential oil is cyclodextrin encapsulated essential oil, and preferred cyclodextrin is a beta-schardinger dextrin-.
The present invention also relates to the application of above-mentioned culicifuge in mosquito repellent on the other hand.
Mosquito repellent product repelling effect of the present invention is good, and toxicity is low, is suitable for family and uses, and particularly is fit to pregnant woman and children and uses.
Embodiment
Embodiment 1
Embodiment one: the preparation of plants essential oil
Get dry lavender, daphne odera, curcuma zedoary, tarragon and camphortree leaf respectively; Extract with supercritical carbon dioxide low-temperature extraction method, concrete steps are following: after respectively each raw material being cleaned up also fragmentation, put into extractor; Improve the pressure of extractor and remain on uniform temperature, thereby make CO
2Liquefaction; At supercritical liq CO
2In the continuous extraction process, the grease constituents in the raw material is dissolved in liquid CO
2In, become mixed solution and flow away, and be insoluble to CO
2Solid slag then be trapped in the extractor; Resulting mixed solution flows in the splitter, makes CO through cooling, step-down
2Generating gasification, grease constituents ooze out, thereby realize Oil-gas Separation, wherein gas CO
2Recycling use, the grease constituents is trapped in the splitter to be removed; Receive the grease constituents in the splitter,, after making with extra care, obtain the essential oil of each raw material with filtering behind the anhydrous sodium sulfate drying.
Embodiment two: the preparation of essential oil inclusion compound
Take by weighing beta-schardinger dextrin-(through 50 ℃ of dry 2h) and put into conical flask in right amount, add an amount of distilled water then, heating for dissolving is processed the beta-schardinger dextrin-saturated aqueous solution.After the cooling, place on the magnetic stirring apparatus, slowly add the equal-volume mixed solution of above-mentioned essential oil at a certain temperature, stir inclusion certain hour (2g), leave standstill, refrigeration 24h filters.With the washing with alcohol of 100mL 95%, filter, hang, 40 ℃ of dry 2h promptly get inclusion compound in vacuum drying chamber.
Test Example, mosquito repellent test
With the positive contrast of 95% Metadelphene crude oil (commercially available), with ethanol as blank.
With reference to the State Standard of the People's Republic of China, the indoor harmacological effect of agriculture chemical registration hygienic insecticide is estimated the method for repellant (GB/T17355.10-1998) and is carried out the mosquito repellent experiment.
Attack test: growing * wide * high for putting into the female mosquito of aedes albopictus that 300 4~5 ages in days are not sucked blood in the mosquito cage of 30cm * 30cm * 60cm.After wiping out mouse web portion and becoming mildewed, hair remover is applied to belly, paper towel is wiped behind the 5min, rinses well with running water, dries with cotton balls.The depilation time should be not long, otherwise can destroy the skin of mouse.Test mice is fixed in the plastic plate that folds up mouse, and belly is aimed at the border circular areas (diameter 2.5cm) that is put in hollow out, and four limbs and tail cover skin with the tight winding of adhesive tape.To try mouse and put into mosquito cage 2min, the examination worm that comes to stop falling is qualified for attack more than 20 persons, and this mouse and this cage mosquito can be used for repellency test.
Mosquito repellent test: get 12 mouse and divide 4 groups, handle with above-mentioned method depilation.On the skin of abdomen that mouse exposes from border circular areas (diameter 2.5cm) (losing hair or feathers); Evenly smear the essential oil, the embodiment of the invention 2 manufactured goods (0.1mL), Metadelphene crude oil 20 μ L and the absolute ethyl alcohol 20 μ L that supply examination essential oil inclusion compound (0.1g) or equal-volume to mix respectively, will try mouse and put into mosquito cage (300 female mosquitos in the left and right sides are arranged) respectively, observe 2min; The mosquito quantity that the record thorn is stung mouse; Whether every interval 1h puts into the observation of mosquito cage has bite by mosquitos, observes 5h altogether, observes 2min at every turn.The mouse of smearing 20 μ L absolute ethyl alcohols with belly makes the blank group, observes the attacking ability of mosquito.Calculate the Effective Vate of Protection of each group, computing formula is following:
Effective Vate of Protection (%)=(contrast mosquito thorn sting and count-be coated with test sample after mosquito thorn sting number)/blank group mosquito is stung and stings number.Experimental result such as table 1:
Wherein: experimental group 1 is the mixed essential oil group; Experimental group 2 is essential oil inclusion compound groups; Positive controls is a repellent amine crude oil; Blank control group: absolute ethyl alcohol.
Embodiment four: toxicological experiment
Prepared Chinese medicine blend of essential oils and essential oil inclusion compound carry out acute toxicity experience, and the result is following:
The oral acute toxicity experience of mouse: the LD of the oral blend of essential oils of mouse
50Be 105g/kg, the LD50 of essential oil inclusion compound is 115g/kg, 95% the credible 159.28g/kg~228.63g/kg that is limited to.And the toxicity criterion of air freshener is: the malicious LD of the oral urgency of mouse
50>=5g/kg is judged to be nontoxic.The LD of this Chinese medicine essential oil
50Be higher than 21 times of nontoxic standard, so be judged to be nontoxic.
When being understood that, above-mentioned embodiment only is an illustrative, concerning those of ordinary skills, can improve or conversion according to above-mentioned explanation, and all these improvement and conversion all should belong to the protection domain of accompanying claims of the present invention.