CN102690799A - 具有脱卤素酶活性的酶及其使用方法 - Google Patents

具有脱卤素酶活性的酶及其使用方法 Download PDF

Info

Publication number
CN102690799A
CN102690799A CN2012101536411A CN201210153641A CN102690799A CN 102690799 A CN102690799 A CN 102690799A CN 2012101536411 A CN2012101536411 A CN 2012101536411A CN 201210153641 A CN201210153641 A CN 201210153641A CN 102690799 A CN102690799 A CN 102690799A
Authority
CN
China
Prior art keywords
sequence
polypeptide
nucleic acid
dna
group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2012101536411A
Other languages
English (en)
Chinese (zh)
Inventor
J·M·肖特
T·理查森
D·罗伯逊
K·格雷
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Diversa Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diversa Corp filed Critical Diversa Corp
Publication of CN102690799A publication Critical patent/CN102690799A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10Sequence alignment; Homology search
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S422/00Chemical apparatus and process disinfecting, deodorizing, preserving, or sterilizing
    • Y10S422/90Decreasing pollution or environmental impact

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Processing Of Solid Wastes (AREA)
CN2012101536411A 2000-12-01 2001-11-30 具有脱卤素酶活性的酶及其使用方法 Pending CN102690799A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25089700P 2000-12-01 2000-12-01
US60/250,897 2000-12-01

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN018222587A Division CN1610754B (zh) 2000-12-01 2001-11-30 具有脱卤素酶活性的酶及其使用方法

Publications (1)

Publication Number Publication Date
CN102690799A true CN102690799A (zh) 2012-09-26

Family

ID=22949600

Family Applications (2)

Application Number Title Priority Date Filing Date
CN2012101536411A Pending CN102690799A (zh) 2000-12-01 2001-11-30 具有脱卤素酶活性的酶及其使用方法
CN018222587A Expired - Fee Related CN1610754B (zh) 2000-12-01 2001-11-30 具有脱卤素酶活性的酶及其使用方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN018222587A Expired - Fee Related CN1610754B (zh) 2000-12-01 2001-11-30 具有脱卤素酶活性的酶及其使用方法

Country Status (9)

Country Link
US (4) US7078504B2 (enExample)
EP (1) EP1507870A4 (enExample)
JP (4) JP4589602B2 (enExample)
KR (1) KR20030051897A (enExample)
CN (2) CN102690799A (enExample)
BR (1) BR0115875A (enExample)
CA (2) CA2430192C (enExample)
MX (1) MXPA03004840A (enExample)
WO (1) WO2002068583A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120924518A (zh) * 2025-08-06 2025-11-11 湖南师范大学 一种卤代烷烃脱卤酶hld-1405及其编码基因与应用

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002537762A (ja) * 1999-01-05 2002-11-12 トラスティーズ オブ ボストン ユニバーシティ 改良型核酸クローニング
US20040005673A1 (en) * 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
US7435562B2 (en) * 2000-07-21 2008-10-14 Modular Genetics, Inc. Modular vector systems
JP4589602B2 (ja) 2000-12-01 2010-12-01 ヴェレニウム コーポレイション デハロゲナーゼ活性を有する酵素およびその利用方法
JP2006512086A (ja) * 2002-09-20 2006-04-13 ダイヴァーサ コーポレイション スタチンおよびスタチン中間体の合成のための酵素化学的方法
US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
EP1594962B1 (en) 2003-01-31 2014-08-27 Promega Corporation Covalent tethering of functional groups to proteins
US7425436B2 (en) * 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US7485423B2 (en) 2004-11-11 2009-02-03 Modular Genetics, Inc. Ladder assembly and system for generating diversity
US7632666B2 (en) * 2004-12-27 2009-12-15 Masarykova Univerzita V Brne Method of production of optically active halohydrocarbons and alcohols using hydrolytic dehalogenation catalysed by haloalkane dehalogenases
CA2667697A1 (en) * 2006-10-30 2008-05-08 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
WO2011137368A2 (en) 2010-04-30 2011-11-03 Life Technologies Corporation Systems and methods for analyzing nucleic acid sequences
US9268903B2 (en) 2010-07-06 2016-02-23 Life Technologies Corporation Systems and methods for sequence data alignment quality assessment
CN102296046B (zh) * 2011-09-16 2013-08-14 广州市暨源生物科技有限公司 可用于预防慢性重金属中毒的重组食品级乳酸菌、其制备方法与应用
EP3107554B1 (en) * 2014-02-19 2020-07-22 University of Tennessee Research Foundation Antibody for skewing sex ratio and methods of use thereof
CN104694514B (zh) * 2015-03-18 2017-11-28 中国科学院微生物研究所 一种脱卤酶DhmB及其编码基因与应用
US10344286B2 (en) 2015-05-13 2019-07-09 Samsung Electronics Co., Ltd. Microorganism including gene encoding protein having hydroxylase activity and method of reducing concentration of fluorinated methane in sample using the same
US10358632B2 (en) 2015-12-07 2019-07-23 Samsung Electronics Co., Ltd. Bacterial cytochrome P450 protein variant and method of reducing concentration of fluorinated methane in sample using the same
CN109706139A (zh) * 2019-03-01 2019-05-03 中国人民解放军92609部队 脱卤素酶基因LinB、脱卤素酶、脱卤素酶基因工程菌及其构建方法和应用方法
CN114574360B (zh) * 2020-12-01 2025-02-11 中国科学院大连化学物理研究所 基于石英内衬管阵列的细胞操作装置及方法
US11760793B2 (en) 2020-12-18 2023-09-19 University Of Tennessee Research Foundation Antibody for skewing sex ratio and methods of use thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1252100A (zh) * 1997-02-13 2000-05-03 唐化学原料公司 重组卤代脂族脱卤素酶
WO2000058517A1 (en) * 1999-03-26 2000-10-05 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5108892A (en) * 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
US6037465A (en) * 1994-06-14 2000-03-14 Invitek Gmbh Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials
US5723125A (en) * 1995-12-28 1998-03-03 Tanox Biosystems, Inc. Hybrid with interferon-alpha and an immunoglobulin Fc linked through a non-immunogenic peptide
US6183740B1 (en) * 1997-08-13 2001-02-06 Diversa Corporation Recombinant bacterial phytases and uses thereof
JP2002531060A (ja) * 1998-09-29 2002-09-24 ディベルサ コーポレーション Cenarchaeumsymbiosum由来の核酸およびタンパク質
CA2391626A1 (en) * 2000-09-15 2002-03-21 Diversa Corporation Combinatorial screening of mixed populations of organisms
JP4589602B2 (ja) 2000-12-01 2010-12-01 ヴェレニウム コーポレイション デハロゲナーゼ活性を有する酵素およびその利用方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1252100A (zh) * 1997-02-13 2000-05-03 唐化学原料公司 重组卤代脂族脱卤素酶
WO2000058517A1 (en) * 1999-03-26 2000-10-05 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
蔡宝立 等: "嗜甲基菌DM11菌株的二氯甲烷脱卤素酶是二聚体蛋白", 《南开大学学报(自然科学)》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120924518A (zh) * 2025-08-06 2025-11-11 湖南师范大学 一种卤代烷烃脱卤酶hld-1405及其编码基因与应用
CN120924518B (zh) * 2025-08-06 2026-04-21 湖南师范大学 一种卤代烷烃脱卤酶hld-1405及其编码基因与应用

Also Published As

Publication number Publication date
CA2430192C (en) 2014-06-03
WO2002068583A2 (en) 2002-09-06
EP1507870A4 (en) 2005-11-09
US7078504B2 (en) 2006-07-18
MXPA03004840A (es) 2008-10-08
JP4589602B2 (ja) 2010-12-01
KR20030051897A (ko) 2003-06-25
US20060275804A1 (en) 2006-12-07
US20060035216A1 (en) 2006-02-16
JP2010268796A (ja) 2010-12-02
US20130115675A1 (en) 2013-05-09
CN1610754B (zh) 2012-07-04
EP1507870A2 (en) 2005-02-23
CA2846761A1 (en) 2002-09-06
US20100112666A1 (en) 2010-05-06
US8153411B2 (en) 2012-04-10
WO2002068583A3 (en) 2004-12-02
JP5697365B2 (ja) 2015-04-08
JP2015107123A (ja) 2015-06-11
BR0115875A (pt) 2005-05-10
JP2008142089A (ja) 2008-06-26
CN1610754A (zh) 2005-04-27
US7671189B2 (en) 2010-03-02
CA2430192A1 (en) 2002-09-06
US9453211B2 (en) 2016-09-27
JP2005501515A (ja) 2005-01-20

Similar Documents

Publication Publication Date Title
JP5697365B2 (ja) デハロゲナーゼ活性を有する酵素およびその利用方法
Hill et al. Investigation of two evolutionarily unrelated halocarboxylic acid dehalogenase gene families
Strous et al. Deciphering the evolution and metabolism of an anammox bacterium from a community genome
CN104593394A (zh) 具有α淀粉酶活性的酶及其使用方法
Whitworth et al. Myxobacterial genomics and post-genomics: a review of genome biology, genome sequences and related ‘omics studies
Černoša et al. Virulence traits and population genomics of the black yeast Aureobasidium melanogenum
Pérez-Cataluña et al. Arcobacter lacus sp. nov. and Arcobacter caeni sp. nov., two novel species isolated from reclaimed water
US20080227132A1 (en) Hydrolase Enzymes and Their Use in Kinetic Resolution
JP4261799B2 (ja) 鏡像異性的に純粋なα−置換カルボン酸の製造方法
Artuso et al. Phylogenomic reconstruction and metabolic potential of the genus Aminobacter
JP2004523211A (ja) 高温ポリメラーゼ活性を有する酵素およびその使用方法
US20020160476A1 (en) Nucleic acids and proteins from cenarchaeum symbiosum
Zhang et al. Inactivation of target RNA cleavage of a III-B CRISPR-Cas system induces robust autoimmunity in Saccharolobus islandicus
AU2006235875B8 (en) Enzymes having dehalogenase activity and methods of use thereof
Fan et al. The efficient PAE-degrading performance and complete genome sequencing of gordonia sp. LUNF6
Yang et al. Comparative Genomics Reveals Evidence of the Genome Reduction and Metabolic Potentials of Aliineobacillus hadale Isolated from Challenger Deep Sediment of the Mariana Trench
Song et al. Description of Nesterenkonia aerolata sp. nov., an actinobacterium isolated from air of manufacturing shop in a pharmaceutical factory
Dec et al. Prophage φEr670 and Genomic Island GI_Er147 as Carriers of Resistance Genes in Erysipelothrix rhusiopathiae Strains
AU2001297671A1 (en) Enzymes having dehalogenase activity and methods of use thereof
Wdowiak-Wróbel et al. Phenotypic, Chemotaxonomic, and Genome-Based Classification of Phyllobacterium Strains: Two Proposed Novel Species, Phyllobacterium chamaecytisi sp. nov. and Phyllobacterium lublinensis sp. nov
Hu et al. Complete genome sequence of the novel Antarctic Oceanisphaera sp. IT1–181 that carried five plasmids
Chen et al. Genome Mining of Acinetobacter nosocomialis J2 Using Artificial Intelligence Reveals a Highly Efficient Acid Phosphatase for Phosphate Solubilisation
Tocchini-Valentini et al. Avatar pre-tRNAs help elucidate the properties of tRNA-splicing endonucleases that produce tRNA from permuted genes

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C53 Correction of patent of invention or patent application
CB02 Change of applicant information

Address after: American California

Applicant after: BASF Enzymes LLC

Address before: American California

Applicant before: Diversa Corp.

COR Change of bibliographic data

Free format text: CORRECT: APPLICANT; FROM: DIVERSA CORP. TO: BASF ENZYMES LLC

RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20120926