CN102690146B - A kind of culture medium prescription and preparation technology who cultivates medicinal fungi tabasheer - Google Patents
A kind of culture medium prescription and preparation technology who cultivates medicinal fungi tabasheer Download PDFInfo
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- CN102690146B CN102690146B CN201210170572.5A CN201210170572A CN102690146B CN 102690146 B CN102690146 B CN 102690146B CN 201210170572 A CN201210170572 A CN 201210170572A CN 102690146 B CN102690146 B CN 102690146B
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Abstract
Cultivate culture medium prescription and the preparation technology of medicinal fungi tabasheer, culture medium processing technique field. It is characterized in that containing nutrient solution 21-37%, sugar 8-19%, mushroom 2-11%, synergistic component 5-16%, mineral water 30-56%. Above-mentioned a kind of culture medium prescription and preparation technology who cultivates medicinal fungi tabasheer, contain the bamboo parasitic fungus required various nutritional labelings of surviving, be well positioned to meet the required nutriment of growth of bamboo parasitic fungus, shorten cultivation cycle, improve hypocrellin output and quality, and culture effect is good, cultivate the quality of hypocrellin out and occurring in nature tabasheer hypocrellin without obvious difference, this culture medium is simple for production, safe and effective, easy to use, low production cost. This culture medium prescription can be applied on the artificial cultivation in laboratory and industrial large area production development tabasheer hypocrellin.
Description
Technical field
The invention belongs to culture medium processing technique field, be specifically related to a kind of culture medium prescription of cultivating medicinal fungi tabasheerAnd preparation technology.
Background technology
Tabasheer is Hypocreaceae bacterial parasite, and its stroma is warm in nature lightly seasoned, be commonly called as bamboo, the red dumpling of bamboo, bamboo pseudo-ginseng, XUESANQI,Bamboo ginseng, bamboo cocoon or bitter bamboo, have cough-relieving apophlegmatic, stimulate the circulation of the blood and cause the muscles and joints to relax, expelling wind and removing dampness, tonifying middle-Jiao and Qi, blood circulation promoting and enriching, the stasis of blood of faling apart stimulate the menstrual flow workEffect that blood, ying-qi are defended blood, broken stasis of blood pain relieving, recovery organization function, built up health, cures mainly cold of insufficiency type and stomachache, rheumatic arthritis hundredDay cough, the disease such as sciatica, traumatic injury, muscles and bones are ached, numb limb, back strain, anaemia headache. In the time of Ming Dynasty LeeIn precious monumental work Compendium of Material Medica, just there is following record: " tabasheer, have another name called Tabasheer, taste is sweet, cold, nontoxic. Cure mainly child convulsion,Remove wind-heat, relieving palpitation improving eyesight, nourish the five internal organs ".
Multiplex tabasheer infusing drugs in wine among the people, be used for the treatment of rheumatic arthritis, sciatica, lumbar muscle strain, traumatic injury andCold of insufficiency type and stomachache, child convulsion, oxyhepatitis etc. are the conventional important Chinese medicine resources of China. Tabasheer is in the bamboo grove of different habitatsParasitic situation also have very big difference: in the bamboo grove in corrie depression or the moistening shady and cool habitat of Schattenseite, parasitic rate is the highest, pureParasitic rate in bamboo grove is higher than the parasitic rate in mixed forest. Mean parasitized rate is respectively short fringe bamboo 18.47%, bitter bamboo 11.26%, thunderBamboo 0.83%, an ancient plucked stringed instrument bamboo 3.33%, wherein bitter bamboo belongs to for New Host Records. Annual late May to early June is tabasheer optimum growhPuberty is also its optimal harvest time. Bamboo parasitic fungus can be 22 DEG C of-28 DEG C of growths. 22 DEG C-25 of the growth optimum temperatures of myceliaDEG C, humidity is about 65%-75%. Fruit body development is at 25 DEG C-28 DEG C, and more than 80% humidity of need, more than rain in springRear generation. Tabasheer grows needs scattered light, and direct sunlight virgin entity occurs less. Tabasheer is mainly distributed in Zhejiang, fourThe Southeast China quadrate parts such as river, Jiangxi, Jiangsu, Hubei, Hunan, Anhui, Guizhou, Fujian, Yunnan divide province, in addition, in AsiaAlso there is distribution in Japan, and other countries have no report. But along with the urbanization in growing, the rural area of human society, deserted mountainOpen up wasteland the destruction of utilizing with environment, the host plant of bamboo parasitic fungus is fewer and feweri, and then causes microorganism resource to be successively decreased year by year, faces thingPlant the crisis of extinction.
Summary of the invention
For the above-mentioned problems in the prior art, the object of the invention is to design provides a kind of medicinal fungi of cultivatingThe culture medium prescription of tabasheer and preparation technology's technical scheme, its safety non-toxic, simple and effective, easy to use, with low cost, effectFruit is good.
Described a kind of culture medium prescription of cultivating medicinal fungi tabasheer, is characterized in that containing nutrient solution 21-37%, sugarDivide 8-19%, mushroom 2-11%, synergistic component 5-16%, mineral water 30-56%;
Described nutrient solution is that the equivalent leaf of bamboo, fresh corn, apple, purple potato, Poria cocos are squeezed the juice and made; Described sugar is maltosemalt sugarOne in sugar, brown sugar, mannose, fructose, maltose, galactolipin, lactose, wood sugar, glucose, sucrose, rhamnose or a kind ofAbove mixture; Described mushroom is saccharomycete, hay bacillus, Bifidobacterium, Lactobacillus acidophilus, Raman lactobacillus, wax sample budOne or more mixtures in spore bacillus, intermediary streptococcus, Lactobacillus rhamnosus; Described synergistic component is nitric acidOne or one in potassium, sodium nitrate, magnesium sulfate, dipotassium hydrogen phosphate, ferrous sulfate, ammonium nitrate, ammonium sulfate, copper sulphate, sodium molybdatePlant above mixture.
Described a kind of culture medium prescription of cultivating medicinal fungi tabasheer, is characterized in that the content of described nutrient solution is23-34%, is preferably 27-31%.
Described a kind of culture medium prescription of cultivating medicinal fungi tabasheer, the content that it is characterized in that described sugar is 9-16%, preferably 11-15%.
Described a kind of culture medium prescription of cultivating medicinal fungi tabasheer, the content that it is characterized in that described mushroom is 3-10%, be preferably 5-9%.
Described a kind of culture medium prescription of cultivating medicinal fungi tabasheer, is characterized in that the content of described synergistic component is7-15%, is preferably 8-13%.
Described a kind of culture medium prescription of cultivating medicinal fungi tabasheer, is characterized in that the content of described mineral water is31-53, is preferably 32-49%.
The preparation technology of described a kind of culture medium prescription of cultivating medicinal fungi tabasheer, is characterised in that and comprises following stepRapid:
1) take the equivalent leaf of bamboo, fresh corn, apple, purple potato, Poria cocos, pulverize to mash and squeeze the juice, remove and remove slag by 4 layers of filtered through gauzeDregs, collection juice mixes and forms nutrient solution, for subsequent use;
2) be nutrient solution 21-37%, sugar 8-19%, mushroom 2-11%, synergistic component 5-16%, ore deposit according to weight percentageThe ratio of spring 30-56% takes above-mentioned substance, and mixing stirs, and in 120-135 DEG C of sterilizing 20-50min, obtains trainingSupport base.
The preparation technology of described a kind of culture medium prescription of cultivating medicinal fungi tabasheer, is characterised in that step 2) in: go outBacterium temperature is 125-132 DEG C, preferably 128-130 DEG C; Sterilization time is 25-45min, preferably 30-40min.
Above-mentioned a kind of culture medium prescription and preparation technology who cultivates medicinal fungi tabasheer, contains required each of bamboo parasitic fungus existencePlant nutritional labeling, be well positioned to meet the required nutriment of growth of bamboo parasitic fungus, shorten cultivation cycle, improve hypocrellin output and matterAmount, and culture effect is good, cultivate tabasheer out with the quality of occurring in nature tabasheer without obvious difference, this culture medium is made simpleJust, safe and effective, easy to use, low production cost. This culture medium prescription can manually be cultivated and industrial large in laboratoryOn long-pending production development tabasheer hypocrellin, apply.
Unless otherwise indicated, other the weight percent that is pure material contains the percentage composition relating in present specificationAmount.
Detailed description of the invention
Below by specific embodiment, the invention will be further described, and further illustrate the present invention in conjunction with the test of pesticide effectivenessBeneficial effect.
Embodiment 1
In 300L reactor, drop into 147kg mineral water, then it is fresh to drop into the 16.2kg leaf of bamboo juice, the 16.2kg that pulverize after squeezing the juiceCorn juice, 16.2kg cider, the purple potato juice of 16.2kg and 16.2kg Poria cocos juice, then drop into 3kg maltose, 10kg brown sugar, 10kgMannose and 10kg rhamnose, then drop into 3kg Lactobacillus acidophilus, 3kg Raman lactobacillus, 3kg bacillus cereus, 3kg intermediaryStreptococcus and 3kg Lactobacillus rhamnosus, stir, more slowly drop into 4kg dipotassium hydrogen phosphate, 4kg ferrous sulfate, 4kgAmmonium nitrate, 4kg ammonium sulfate, 4kg copper sulphate and 4kg sodium molybdate, finally stir to total material again and dissolve completely, in 128DEG C sterilizing 30min, final culture medium.
Embodiment 2
In 300L reactor, drop into 96kg mineral water, then it is fresh to drop into the 18.6kg leaf of bamboo juice, the 18.6kg that pulverize after squeezing the juiceCorn juice, 18.6kg cider, the purple potato juice of 18.6kg and 18.6kg Poria cocos juice, then drop into 5kg fructose, 5kg maltose, 5kgGalactolipin, 5kg lactose, 5kg wood sugar, 10kg glucose, 8kg sucrose and 2kg rhamnose, then drop into 7kg saccharomycete, 5kg withered grassBacillus, 5kg Bifidobacterium, 2kg Lactobacillus acidophilus, 2kg Raman lactobacillus, 2kg bacillus cereus, 2kg intermediary streptococcus and2kg Lactobacillus rhamnosus, stirs, more slowly drops into 9kg potassium nitrate, 6kg sodium nitrate, 6kg magnesium sulfate, 6kg phosphoric acidHydrogen dipotassium, 6kg ferrous sulfate and 6kg sodium molybdate, finally stir to total material again and dissolve completely, in 129 DEG C of sterilizings32min, final culture medium.
Embodiment 3
In 300L reactor, drop into 117kg mineral water, then drop into 18kg leaf of bamboo juice, the fresh corn of 18kg pulverized after squeezing the juiceJuice, 18kg cider, the purple potato juice of 18kg and 18kg Poria cocos juice, then drop into 2kg maltose, 4kg brown sugar, 4kg mannose, 4kg fruitSugar, 4kg maltose, 4kg galactolipin, 4kg lactose, 4kg wood sugar, 4kg glucose, 4kg sucrose and 4kg rhamnose, then drop into 5kgSaccharomycete, 5kg hay bacillus, 5kg Bifidobacterium, 5kg intermediary streptococcus and 5kg Lactobacillus rhamnosus, stir, thenSlowly drop into 6kg potassium nitrate, 4kg sodium nitrate, 4kg magnesium sulfate, 4kg ammonium sulfate, 4kg copper sulphate and 4kg sodium molybdate, finally stir againMix evenly to total material and dissolve completely, in 130 DEG C of sterilizing 34min, final culture medium.
Embodiment 4
In 300L reactor, drop into 129kg mineral water, then it is fresh to drop into the 16.6kg leaf of bamboo juice, the 16.6kg that pulverize after squeezing the juiceThe purple potato juice of corn juice, 16.6kg cider, 16.6kg and 16.6kg Poria cocos juice, then drop into 5kg maltose, 5kg brown sugar, 5kg sweetReveal sugar, 5kg fructose, 5kg maltose, 5kg galactolipin, 5kg lactose and 5kg rhamnose, then drop into 2kg saccharomycete, 2kg withered grass barBacterium, 2kg Bifidobacterium, 2kg Lactobacillus acidophilus, 2kg Raman lactobacillus, 5kg bacillus cereus and 5kg Lactobacillus rhamnosus,Stir, more slowly drop into 4kg potassium nitrate, 4kg sodium nitrate, 5kg magnesium sulfate, 5kg dipotassium hydrogen phosphate, 2kg sulfuric acid AsiaIron, 2kg ammonium nitrate, 2kg ammonium sulfate, 2kg copper sulphate and 2kg sodium molybdate, finally stir to total material again and dissolve completely,In 128.5 DEG C of sterilizing 36min, final culture medium.
Embodiment 5
In 300L reactor, drop into 131kg mineral water, then drop into 17kg leaf of bamboo juice, the fresh corn of 17kg pulverized after squeezing the juiceJuice, 17kg cider, the purple potato juice of 17kg and 17kg Poria cocos juice, then drop into 5kg maltose, 5kg brown sugar, 5kg mannose, 4kg fruitSugar, 4kg wood sugar, 4kg glucose, 4kg sucrose and 4kg rhamnose, then drop into 7kg saccharomycete, 2kg hay bacillus, 2kg and have a liking for lactic acidBacillus, 2kg Raman lactobacillus, 2kg bacillus cereus and 2kg intermediary streptococcus, stir, more slowly drop into 2kgPotassium nitrate, 5kg sodium nitrate, 5kg magnesium sulfate, 5kg ferrous sulfate, 5kg ammonium nitrate, 5kg ammonium sulfate and 5kg copper sulphate, more finallyStir to total material and dissolve completely, in 129.5 DEG C of sterilizing 38min, final culture medium.
Embodiment 6
In 300L reactor, drop into 118kg mineral water, then it is fresh to drop into the 17.6kg leaf of bamboo juice, the 17.6kg that pulverize after squeezing the juiceThe purple potato juice of corn juice, 17.6kg cider, 17.6kg and 17.6kg Poria cocos juice, then drop into 2kg maltose, 5kg brown sugar, 5kg sweetReveal sugar, 5kg fructose, 4kg maltose, 4kg galactolipin, 4kg lactose, 4kg wood sugar and 4kg glucose, then drop into 2kg withered grass barBacterium, 5kg Bifidobacterium, 5kg Lactobacillus acidophilus, 5kg Raman lactobacillus and 5kg bacillus cereus, stir, slowerSlow 5kg sodium nitrate, 6kg magnesium sulfate, 6kg dipotassium hydrogen phosphate, 6kg ammonium nitrate, 6kg ammonium sulfate and the 6kg sodium molybdate of dropping into, more finallyStir to total material and dissolve completely, in 129.3 DEG C of sterilizing 40min, final culture medium.
Further illustrate below the excellent results of above-mentioned culture medium prescription by test explanation.
The result of the test that table 1 embodiment 1-6 cultivates bamboo parasitic fungus
Table 1 shows: embodiment 1-6 all has good effect to the cultivation of bamboo parasitic fungus, and embodiment 1 is better than embodiment 2, implementsExample 3 is better than embodiment 4, and embodiment 6 is better than embodiment 5.
Claims (10)
1. cultivate a culture medium for medicinal fungi tabasheer, it is characterized in that containing nutrient solution 21-37%, sugar 8-19%, mushroom2-11%, synergistic component 5-16%, mineral water 30-56%;
Described nutrient solution is that the equivalent leaf of bamboo, fresh corn, apple, purple potato, Poria cocos are squeezed the juice and made; Described sugar is maltose, redOne or more mixtures in sugar, mannose, fructose, maltose, galactolipin, lactose, wood sugar, sucrose, rhamnose; InstituteThe mushroom of stating is saccharomycete, hay bacillus, Bifidobacterium, Lactobacillus acidophilus, Raman lactobacillus, bacillus cereus, intermediary's chainOne or more mixtures in coccus, Lactobacillus rhamnosus; Described synergistic component is potassium nitrate, sodium nitrate, phosphoric acidOne or more mixtures in hydrogen dipotassium, ferrous sulfate, ammonium nitrate, ammonium sulfate, copper sulphate, sodium molybdate;
This culture medium adopts following methods preparation:
1) take the equivalent leaf of bamboo, fresh corn, apple, purple potato, Poria cocos, pulverize and mash and squeeze the juice, remove dregs by 4 layers of filtered through gauze,Collection juice mixes and forms nutrient solution, for subsequent use;
2) be nutrient solution 21-37%, sugar 8-19%, mushroom 2-11%, synergistic component 5-16%, mineral water according to weight percentageThe ratio of 30-56% takes above-mentioned substance, and mixing stirs, and in 120-135 DEG C of sterilizing 20-50min, obtains culture medium.
2. a kind of culture medium of cultivating medicinal fungi tabasheer as claimed in claim 1, is characterized in that containing of described nutrient solutionAmount is 23-34%.
3. a kind of culture medium of cultivating medicinal fungi tabasheer as claimed in claim 1, is characterized in that containing of described nutrient solutionAmount is 27-31%.
4. a kind of culture medium of cultivating medicinal fungi tabasheer as claimed in claim 1, is characterized in that the content of described sugarFor 9-16%.
5. a kind of culture medium of cultivating medicinal fungi tabasheer as claimed in claim 1, is characterized in that the content of described sugarFor 11-15%.
6. a kind of culture medium of cultivating medicinal fungi tabasheer as claimed in claim 1, is characterized in that the content of described mushroomFor 3-10%.
7. a kind of culture medium of cultivating medicinal fungi tabasheer as claimed in claim 1, is characterized in that the content of described mushroomFor 5-9%.
8. a kind of culture medium of cultivating medicinal fungi tabasheer as claimed in claim 1, is characterized in that described synergistic componentContent is 7-15%.
9. a kind of culture medium of cultivating medicinal fungi tabasheer as claimed in claim 1, is characterized in that containing of described mineral waterAmount is 31-53%.
10. a kind of culture medium of cultivating medicinal fungi tabasheer as claimed in claim 1, is characterised in that preparation method's step 2)In: sterilising temp is 125-132 DEG C, and sterilization time is 25-45min.
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| CN108341715A (en) * | 2018-03-28 | 2018-07-31 | 安徽帝元生物科技有限公司 | Composition and its preparation method and application |
| CN113648685A (en) * | 2021-08-18 | 2021-11-16 | 唐山华油微生物科技开发有限公司 | Efficient composite microbial demulsifier and application thereof |
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| CN1129254A (en) * | 1995-02-17 | 1996-08-21 | 周道陆 | Composite granulated substratum for edible (medicinal) mushroom series |
| CN101176409A (en) * | 2007-12-06 | 2008-05-14 | 田发建 | Cultivating method for root art bamboo miniascape |
| CN101186932A (en) * | 2007-11-11 | 2008-05-28 | 杨海龙 | Method for synchronously producing hypocrellin and shiraia bambusicola polysaccharides |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2010233535A (en) * | 2009-03-31 | 2010-10-21 | Daiwarandam Co Ltd | Mushroom cultivation method, culture medium, and mushroom |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1129254A (en) * | 1995-02-17 | 1996-08-21 | 周道陆 | Composite granulated substratum for edible (medicinal) mushroom series |
| CN101186932A (en) * | 2007-11-11 | 2008-05-28 | 杨海龙 | Method for synchronously producing hypocrellin and shiraia bambusicola polysaccharides |
| CN101176409A (en) * | 2007-12-06 | 2008-05-14 | 田发建 | Cultivating method for root art bamboo miniascape |
Non-Patent Citations (1)
| Title |
|---|
| 药用竹黄菌的生物学研究进展;贾小明，等;《微生物通报》;20061231;第33卷(第3期);第149页第4节内容 * |
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