CN102670688A - Active ingredient extract of isatis roots and preparation method for active ingredient extract - Google Patents
Active ingredient extract of isatis roots and preparation method for active ingredient extract Download PDFInfo
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- CN102670688A CN102670688A CN2012101590435A CN201210159043A CN102670688A CN 102670688 A CN102670688 A CN 102670688A CN 2012101590435 A CN2012101590435 A CN 2012101590435A CN 201210159043 A CN201210159043 A CN 201210159043A CN 102670688 A CN102670688 A CN 102670688A
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Abstract
The invention discloses an active ingredient extract of isatis roots and a preparation method for the active ingredient extract. According to the preparation method for the active ingredient extract, the conventional water extraction and alcohol sedimentation technology is modified into a clarifier treatment technology; and when impurities are removed, active ingredients of the medicament are well preserved, and the yield of thick paste is improved. Process conditions and parameters of a clarifier are specifically researched, and the technical scheme of the invention is obtained; the problems that the sediment is fine and difficult to separate because the using conditions of the clarifier are unreasonable or the sediment is not separately thoroughly because the category and the consumption of the clarifier are unreasonable are solved; and compared with the prior art, the preparation method has the advantage of remarkable progress.
Description
Technical field
The present invention relates to field of medicaments, be specifically related to a kind of Radix Isatidis active component extract and preparation method thereof.
Background technology
Radix Isatidis has heat-clearing and toxic substances removing, the removing heat from blood sore-throat relieving, and the effect of detumescence is used for hyperactivity of toxic heat, laryngopharynx swelling and pain, tonsillitis, parotitis.Differential diagnosis in tcm is sore throat (a wind heat disease), and disease is seen pharyngalgia, dryness and heat, and dysphagia, mouth is little thirsty, heating, slight chill, pharyngeal red slightly or scarlet, edema, tongue tip side of red, white and thin fur, floating and rapid pulse.
The preparation technology of the active component extract of Radix Isatidis in the present technology; Belong to the Chinese medicine traditional handicraft, adopt decoction and alcohol sedimentation technique, reconcentration became thick paste after supernatant reclaimed ethanol; The main purpose of precipitate with ethanol is the stability that promotes product for body; Remove impurity, still, when removing impurity, also can remove the part effective ingredient.Problems such as therefore, this method exists amount of ethanol big, and this is higher to become generation, and loss of effective components is bigger, and the thick paste yield is on the low side.
Summary of the invention
The purpose of this invention is to provide Radix Isatidis active component extract and preparation method thereof, this method for preparing when removing impurity, has better kept effective ingredient with traditional alcohol precipitation process replacement becoming clarifier treatment process, has improved the thick paste yield.
Radix Isatidis active component extract of the present invention is to adopt following method preparation:
⑴ it is 1% solution that, the A component water of getting the ZTCI+1 natural clarifying agent are mixed with weight ratio concentration, obtains solution 1, and it is 1% solution that the B component uses 1% acetic acid to be mixed with weight ratio concentration, obtains solution 2;
⑵, get Radix Isatidis, decocte with water 2 times, 2～4 hours for the first time, amount of water was 4～8 times of medical material weight, and 1～3 hour for the second time, amount of water was 4～8 times of medical material weight, and collecting decoction filters, and filtrating is concentrated into 0.5～1 times of medical material weight, obtains solution 3;
⑶, solution 3 is heated to 70～90 ℃, adds the percentage by weight that is equivalent to solution 3 gross weights and be 6%～10% solution 2, stir, keeps 70～90 ℃ temperature 3～4 hours, during this period of time whenever once at a distance from stirring in 20～40 minutes; Add the percentage by weight be equivalent to solution 3 gross weights then and be 2%～5% solution 1, stir, close insulation; Make the solution natural cooling, whenever stirred once, left standstill 4～5 hours at a distance from 20～40 minutes; High speed centrifugation, sediment separate out is got supernatant and is placed 2～4 ℃ of insulations 12～24 hours; Filter, promptly get described active component.
Radix Isatidis active component extract of the present invention is to adopt following method preparation:
⑴ it is 1% solution that, the A component water of getting the ZTCI+1 natural clarifying agent are mixed with weight ratio concentration, obtains solution 1, and it is 1% solution that the B component uses 1% acetic acid to be mixed with weight ratio concentration, obtains solution 2;
⑵, get Radix Isatidis, decocte with water 2 times, 2 hours for the first time, amount of water was 5 times of medical material weight, and 1 hour for the second time, amount of water was 5 times of medical material weight, and collecting decoction filters, and filtrating is concentrated into 0.6 times of medical material weight, obtains solution 3;
⑶, solution 3 is heated to 90 ℃, adds the percentage by weight that is equivalent to solution 3 gross weights and be 8% solution 2, stir, keeps 90 ℃ temperature 3 hours, during this period of time whenever once at a distance from stirring in 30 minutes; Add the percentage by weight be equivalent to solution 3 gross weights then and be 5% solution 1, stir, close insulation; Make the solution natural cooling, whenever stirred once, left standstill 4 hours at a distance from 30 minutes; High speed centrifugation, sediment separate out is got supernatant and is placed 4 ℃ of insulations 12 hours; Filter, promptly get described active component.
Indigowoad root oral liquid of the present invention is to adopt following method preparation:
Get above-mentioned active component; The percentage by weight that add the percentage by weight be equivalent to Radix Isatidis medical material weight and be 15%～50% sucrose, is equivalent to Radix Isatidis medical material weight is 0.3% sorbitol; Add water to 1.3～2 times that are equivalent to Radix Isatidis medical material weight; Mix homogeneously promptly gets described oral liquid.
Indigowoad root oral liquid of the present invention is to adopt following method preparation:
Get above-mentioned active component; The percentage by weight that add the percentage by weight be equivalent to Radix Isatidis medical material weight and be 10%～20% mannitol, is equivalent to Radix Isatidis medical material weight is that 0.2%～0.5% aspartame, the percentage by weight that is equivalent to Radix Isatidis medical material weight are 0.3% sorbitol; Add water to 1.2～2 times that are equivalent to Radix Isatidis medical material weight; Mix homogeneously promptly gets described oral liquid.
Radix Isatidis preparation of the present invention is got above-mentioned active component, through suitable concentrating or dilution, adds suitable adjuvant, can prepare the medical preparation that becomes various routines.
Radix Isatidis is the dry root of cruciferae isatis Isatisindigotica Fort., and excavate autumn, removes silt, dries.Bitter in the mouth, cold in nature.GUIXIN, stomach warp.Has heat-clearing and toxic substances removing, the effect of removing heat from blood sore-throat relieving.Be used for the pestilence seasonal noxious pathogen clinically, heating pharyngalgia, maculae caused by violent heat pathogen, mumps, scarlet fever, major part pestilence, erysipelas, carbuncle.
Radix Isatidis preparation provided by the invention and preparation technology thereof with respect to the disclosed technology of prior art, have simplified technical process, have practiced thrift cost, have improved extracts active ingredients efficient, have promoted the effect of medicine, have overcome the defective of prior art.
ZTCI+1 natural clarifying agent of the present invention is the market existing procucts, becomes the clarification technique company limited just to have this product to sell in positive day such as Beijing.
The present invention studied chitosan, 101-clarifier and ZTC1+1 clarifier in the experimental stage; And the process conditions and the parameter of every kind of clarifier carried out concrete research; Finally drawn technical scheme of the present invention, it is too tiny to have overcome the unreasonable deposition that causes of clarifier service condition, is difficult to separate; Perhaps clarifier kind and consumption unreasonable cause separate problems such as not thorough, have obvious improvement in terms of existing technologies.
The specific embodiment
Embodiment 1
Radix Isatidis 1400g
⑴ it is 1% solution that, the A component water of getting the ZTCI+1 natural clarifying agent are mixed with weight ratio concentration, obtains solution 1, and it is 1% solution that the B component uses 1% acetic acid to be mixed with weight ratio concentration, obtains solution 2;
⑵, get Radix Isatidis, decocte with water 2 times, 2 hours for the first time, amount of water was 6400g, and 1 hour for the second time, amount of water was 6400g, and collecting decoction filters, and filtrating is concentrated into 700g, obtains solution 3;
⑶, solution 3 is heated to 70 ℃, adds the solution 2 of 42g, stir, keeps 70 ℃ temperature 3 hours, during this period of time whenever once at a distance from stirring in 20 minutes; Add the solution 1 of 14g then, stir, close insulation, make the solution natural cooling, whenever stirred once, left standstill 4 hours at a distance from 20 minutes, high speed centrifugation, sediment separate out is got supernatant and is placed 2 ℃ of insulations 12 hours, filters, and promptly gets described active component.
Embodiment 2
Radix Isatidis 1000g
⑴ it is 1% solution that, the A component water of getting the ZTCI+1 natural clarifying agent are mixed with weight ratio concentration, obtains solution 1, and it is 1% solution that the B component uses 1% acetic acid to be mixed with weight ratio concentration, obtains solution 2;
⑵, get Radix Isatidis, decocte with water 2 times, 4 hours for the first time, amount of water was 8000g, and 3 hours for the second time, amount of water was 8000g, and collecting decoction filters, and filtrating is concentrated into 700g, obtains solution 3;
⑶, solution 3 is heated to 80 ℃, adds the solution 2 of 100g, stir, keeps 90 ℃ temperature 4 hours, during this period of time whenever once at a distance from stirring in 40 minutes; Add 50g solution 1 then, stir, close insulation, make the solution natural cooling, whenever stirred once, left standstill 5 hours at a distance from 40 minutes, high speed centrifugation, sediment separate out is got supernatant and is placed 4 ℃ of insulations 24 hours, filters, and promptly gets described active component;
⑷, above-mentioned active component are concentrated into suitable relative density, add suitable amount of sucrose, dextrin, and mix homogeneously is granulated, and drying promptly gets granule of the present invention.
Embodiment 3
Radix Isatidis 1000g
⑴ it is 1% solution that, the A component water of getting the ZTCI+1 natural clarifying agent are mixed with weight ratio concentration, obtains solution 1, and it is 1% solution that the B component uses 1% acetic acid to be mixed with weight ratio concentration, obtains solution 2;
⑵, get Radix Isatidis, decocte with water 2 times, 3 hours for the first time, amount of water was 5000g, and 3 hours for the second time, amount of water was 6000g, and collecting decoction filters, and filtrating is concentrated into 1000g, obtains solution 3;
⑶, solution 3 is heated to 80 ℃, adds the solution 2 of 60g, stir, keeps 80 ℃ temperature 4 hours, during this period of time whenever once at a distance from stirring in 30 minutes; Add 50g solution 1 then, stir, close insulation, make the solution natural cooling, whenever stirred once, left standstill 5 hours at a distance from 30 minutes, high speed centrifugation, sediment separate out is got supernatant and is placed 4 ℃ of insulations 18 hours, filters, and promptly gets described active component;
⑷, above-mentioned active component are concentrated into suitable relative density, add appropriate amount of starch and dextrin, and mix homogeneously is granulated, drying, and tabletting promptly gets tablet of the present invention.
Embodiment 4
Radix Isatidis 1000g
⑴ it is 1% solution that, the A component water of getting the ZTCI+1 natural clarifying agent are mixed with weight ratio concentration, obtains solution 1, and it is 1% solution that the B component uses 1% acetic acid to be mixed with weight ratio concentration, obtains solution 2;
⑵, get Radix Isatidis, decocte with water 2 times, 2 hours for the first time, amount of water was 5000g, and 1 hour for the second time, amount of water was 5000g, and collecting decoction filters, and filtrating is concentrated into 600g, obtains solution 3;
⑶, solution 3 is heated to 80 ℃, adds the solution 2 of 48g, stir, keeps 80 ℃ temperature 4 hours, during this period of time whenever once at a distance from stirring in 30 minutes; Add 30g solution 1 then, stir, close insulation, make the solution natural cooling, whenever stirred once, left standstill 4 hours at a distance from 30 minutes, high speed centrifugation, sediment separate out is got supernatant and is placed 3 ℃ of insulations 16 hours, filters, and promptly gets described active component;
⑷, get active component, add the sucrose of 500g, the sorbitol of 3g, add water to 1300g, mix homogeneously promptly gets oral liquid of the present invention.
Embodiment 5
Radix Isatidis 1000g
⑴ it is 1% solution that, the A component water of getting the ZTCI+1 natural clarifying agent are mixed with weight ratio concentration, obtains solution 1, and it is 1% solution that the B component uses 1% acetic acid to be mixed with weight ratio concentration, obtains solution 2;
⑵, get Radix Isatidis, decocte with water 2 times, 2 hours for the first time, amount of water was 6000g, and 3 hours for the second time, amount of water was 6000g, and collecting decoction filters, and filtrating is concentrated into 700g, obtains solution 3;
⑶, solution 3 is heated to 70 ℃, adds the solution 2 of 42g, stir, keeps 80 ℃ temperature 4 hours, during this period of time whenever once at a distance from stirring in 30 minutes; Add 32g solution 1 then, stir, close insulation, make the solution natural cooling, whenever stirred once, left standstill 5 hours at a distance from 40 minutes, high speed centrifugation, sediment separate out is got supernatant and is placed 3 ℃ of insulations 24 hours, filters, and promptly gets described active component;
⑷, get active component, add the mannitol of 200g, the aspartame of 5g, the sorbitol of 3g, add water to 2000g, mix homogeneously promptly gets described oral liquid.
Embodiment 6
Former Radix Isatidis aqueous extraction-alcohol precipitation technology and the contrast of the embodiment of the invention 1 effective component extracting all are after extracting with Radix Isatidis, and be basic as a comparison at the dried cream that 70 ℃ of following vacuum dryings obtain:
| Dried cream title | Extract the medical material amount | Dried cream weight (g) | Yield (%) |
| Former technology appearance 1 | 10000g | 820 | 8.2 |
| Former technology appearance 1 | 10000g | 805 | 8.05 |
| 1 kind of embodiment | 10000g | 945 | 9.45 |
| 2 kinds of embodiment | 10000g | 985 | 9.85 |
| 3 kinds of embodiment | 10000g | 956 | 9.56 |
Can find out by last table, method for preparing of the present invention, the dried cream yield of the effective ingredient of acquisition has improved more than 10% respectively with respect to former technology.
Claims (5)
1. the active component extract of a Radix Isatidis is characterized in that, is to adopt following method preparation:
⑴ it is 1% solution that, the A component water of getting the ZTCI+1 natural clarifying agent are mixed with weight ratio concentration, obtains solution 1, and it is 1% solution that the B component uses 1% acetic acid to be mixed with weight ratio concentration, obtains solution 2;
⑵, get Radix Isatidis, decocte with water 2 times, 2～4 hours for the first time, amount of water was 4～8 times of medical material weight, and 1～3 hour for the second time, amount of water was 4～8 times of medical material weight, and collecting decoction filters, and filtrating is concentrated into 0.5～1 times of medical material weight, obtains solution 3;
⑶, solution 3 is heated to 70～90 ℃, adds the percentage by weight that is equivalent to solution 3 gross weights and be 6%～10% solution 2, stir, keeps 70～90 ℃ temperature 3～4 hours, during this period of time whenever once at a distance from stirring in 20～40 minutes; Add the percentage by weight be equivalent to solution 3 gross weights then and be 2%～5% solution 1, stir, close insulation; Make the solution natural cooling, whenever stirred once, left standstill 4～5 hours at a distance from 20～40 minutes; High speed centrifugation, sediment separate out is got supernatant and is placed 2～4 ℃ of insulations 12～24 hours; Filter, promptly get described active component.
2. the active component extract of Radix Isatidis according to claim 1 is characterized in that, is to adopt following method preparation:
⑴ it is 1% solution that, the A component water of getting the ZTCI+1 natural clarifying agent are mixed with weight ratio concentration, obtains solution 1, and it is 1% solution that the B component uses 1% acetic acid to be mixed with weight ratio concentration, obtains solution 2;
⑵, get Radix Isatidis, decocte with water 2 times, 2 hours for the first time, amount of water was 5 times of medical material weight, and 1 hour for the second time, amount of water was 5 times of medical material weight, and collecting decoction filters, and filtrating is concentrated into 0.6 times of medical material weight, obtains solution 3;
⑶, solution 3 is heated to 90 ℃, adds the percentage by weight that is equivalent to solution 3 gross weights and be 8% solution 2, stir, keeps 90 ℃ temperature 3 hours, during this period of time whenever once at a distance from stirring in 30 minutes; Add the percentage by weight be equivalent to solution 3 gross weights then and be 5% solution 1, stir, close insulation; Make the solution natural cooling, whenever stirred once, left standstill 4 hours at a distance from 30 minutes; High speed centrifugation, sediment separate out is got supernatant and is placed 4 ℃ of insulations 12 hours; Filter, promptly get described active component.
3. an indigowoad root oral liquid comprises like the active component extract of claim 1 or 2 described Radix Isatidis, it is characterized in that, is to adopt following method preparation:
Get active component extract; The percentage by weight that add the percentage by weight be equivalent to Radix Isatidis medical material weight and be 15%～50% sucrose, is equivalent to Radix Isatidis medical material weight is 0.3% sorbitol; Add water to 1.3～2 times that are equivalent to Radix Isatidis medical material weight; Mix homogeneously promptly gets described oral liquid.
4. an indigowoad root oral liquid comprises like the active component extract of claim 1 or 2 described Radix Isatidis, it is characterized in that, is to adopt following method preparation:
Get active component extract; The percentage by weight that add the percentage by weight be equivalent to Radix Isatidis medical material weight and be 10%～20% mannitol, is equivalent to Radix Isatidis medical material weight is that 0.2%～0.5% aspartame, the percentage by weight that is equivalent to Radix Isatidis medical material weight are 0.3% sorbitol; Add water to 1.2～2 times that are equivalent to Radix Isatidis medical material weight; Mix homogeneously promptly gets described oral liquid.
5. a Radix Isatidis preparation is characterized in that, comprises each active component extract and pharmaceutically acceptable carrier in claim 1 or 2.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101385750A (en) * | 2007-09-14 | 2009-03-18 | 四川渴望生物科技有限公司 | Preparation method of effective ingredient of isatis root |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101385750A (en) * | 2007-09-14 | 2009-03-18 | 四川渴望生物科技有限公司 | Preparation method of effective ingredient of isatis root |
Non-Patent Citations (3)
| Title |
|---|
| 国家药品监督管理局: "《国家药品中药标准（眼科、耳鼻喉科、皮肤科分册）》", 31 December 2002 * |
| 巩喜姣等: "天然澄清剂在冲剂提取液中澄清工艺试验", 《中医药信息》 * |
| 马依努尔.拜克力等: "吸附澄清剂在纯化中药制剂中的应用", 《新疆医科大学学报》 * |
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Application publication date: 20120919 |