CN102670664A - Preparation for preventing and controlling bee fungal diseases - Google Patents
Preparation for preventing and controlling bee fungal diseases Download PDFInfo
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- CN102670664A CN102670664A CN2012101341023A CN201210134102A CN102670664A CN 102670664 A CN102670664 A CN 102670664A CN 2012101341023 A CN2012101341023 A CN 2012101341023A CN 201210134102 A CN201210134102 A CN 201210134102A CN 102670664 A CN102670664 A CN 102670664A
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- lactobacillus
- bifidobacterium
- bacillus
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Abstract
The invention discloses a preparation for preventing and controlling bee fungal diseases. The preparation consists of 0.1 to 2 percent of (E)-N-(6,6-dimethyl heptanoic-2-olefin-4-alkynyl)-N-methyl-1-naphthalene methylamine hydrochloride, 0.1 to 4 percent of beneficial microorganism and 94 to 99.8 percent of soluble starch or saccharides. The beneficial microorganism comprises one or random combination of bacillus subtilis, bacillus licheniformis, bee bifidobacterium, bifidobacterium asteroids, minimum bifidobacterium, bifidobacterium coryneforme, lactobacillus jensenii, lactobacillus amylovorus, lactobacillus plantarum, tiny lactobacillus and lactobacillus bifermentans; and a gram of the beneficial microorganism comprises 10<9> to 5*10<9> viable bacteria. The preparation for preventing and controlling bee fungal diseases is applied to prevention and control of bee or silkworm fungal diseases.
Description
Technical field
The invention belongs to technical field of chemistry, relate to the chemistry and biology pharmacy, be specifically related to a kind of preparation that is applicable to the control bee fungus disease.
Background technology
The modal mycosis of bee larvae is that Chalk is sick.The spore of fungus is distributed in the air, in the pollen, wide range, the infection to bee larvae simultaneously is fast, mortality rate is high.Prior art treatment Chalk is sick to use antibiotic, because antibiotic can remain in the Apis body, can cause certain harm to people's health behind the edible Mel of people; It is developed immunity to drugs to antibiotic.At present, China has banned use of the antibiotic therapy honeybee disease.
Terbinafine is synthetic by Switzerland Bonn Shandeshi company in 1980; In December, 1992 obtained the FDA of united states drug management board approval listing in Britain's Initial Public Offering (Gupta etc., 1997) in 1991; But this medicine external; Also orally-ingestible is described as the kind of only no serious Liver and kidney untoward reaction in the antifungal drug, transfers OTC after 1999 to and sells.Its preparation has multiple dosage forms such as tablet, cream, spray agent, liquid preparation, is present unique in the world a kind of fungicide, can thoroughly kill fungus.
Nowadays terbinafine HCl has been widely used in each fungus-caused dermatosis (like tinea manus and pedis, tinea corporis and cruris etc.) of human body, for oral administration, external that medicine is divided into.In the last few years, terbinafine HCl also extensively had been generalized on one's body some animals (like cat, Canis familiaris L., rabbit etc.), was used to treat some fungus-caused dermatosiss.
2005; Min Weiwei, Mu Zhimei etc. have delivered one piece " terbinafine HCl is to prevention effect Preliminary Report on Experiment of silkworm fungal disease "; Inhibition to silkworm aspergillosis, muscardine, green muscardine is studied; Result of study show when terbinafine HCl add food concentration when reaching 200 μ g/mL, the prevention effect of silkworm aspergillosis, muscardine, green muscardine is reached 100%; The long-time stability experimental result shows that these medicine long-time stability are good.
Terbinafine HCls such as 2,005 31 (2) the silkworms industry science towering Mu Zhi Mei Lijisheng in Min are to the prevention effect Preliminary Report on Experiment of silkworm fungal disease.Its summary announces that terbinafine HCl is 0.0625～0.2726 μ g/mL to the minimal inhibitory concentration scope of silkworm pathogenic fungi, and the MBC scope is 0.125～4 μ g/mL.Biologic test shows that terbinafine HCl is good to the silkworm fungal disease prevention effect, and prevention effect reaches 100% during 200 μ g/mL, and silkworm growth promoter, cocoon quality are had no adverse effects.Stability test shows that the terbinafine HCl long-time stability are good.
But above-mentioned data does not all relate to the control of bee fungus disease.
CN102273495A discloses the medicine of the strong control bee fungus disease of a kind of guarantor honeybee by name; Its component is 11 flavor Chinese herbal medicine such as Flos Lonicerae, Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Isatidis; Be equipped with bromo geramine, through grinding, soak, concentrate, a series of processes such as filtration, technology is comparatively loaded down with trivial details.
CN1251713C discloses a kind of Chinese medicine preparation of preventing and treating honeybee disease, by forming with above-mentioned diverse eight kinds of Chinese herbal medicine, through grinding, pulverize, stir, drying.Also need soak during use, boil, cool off, be blended into Apis, filter, clarify.Use very inconvenience.
CN1142948A discloses a kind of sick new drug-Ke E Ling of bee larvae Chalk of treating, and the trace element that from replenish Apis food, lacks is set about, and improves its resistance against diseases.Find out that from embodiment its cure rate is not 100%, and do not have bactericidal effect.
Summary of the invention
To the above-mentioned defective of present control bee fungus disease especially Chalk medicine, it is obvious to the purpose of this invention is to provide a kind of prevention effect, and processing technology is easy, control bee fungus disease preparation easy to use.
A kind of preparation of preventing and treating bee fungus disease of the present invention is by (E)-N-(6,6-dimethyl hept-2-ene"-4-yl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt, and beneficial microbe and soluble starch or saccharide are formed.
The weight ratio of each component is: (E)-and N-(6,6-dimethyl hept-2-ene"-4-alkynyl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt 0.1～2%, beneficial microbe 0.1～4%, soluble starch or saccharide 94～99.8%.
Said beneficial microbe is: bacillus subtilis, or Bacillus licheniformis, or Apis bacillus bifidus; Or bifidobacterium asteroides, or Bifidobacterium minimum, or bifidobacterium coryneforme; Or Lactobacillus Jensenii, or food starch milk bacillus, or Lactobacillus plantarum; Or small lactobacillus, or two Lactobacillus fermenti, or the combination in any of above-mentioned bacillus; Containing viable count in the said beneficial microbe 0.1～4% is 10
9～5 * 10
9Individual/gram.
Said saccharide is glucose or fructose.
The preparation of control bee fungus disease of the present invention is applied to the control of Apis or silkworm fungal disease.
This preparation composition includes: (E)-and N-(6,6-dimethyl hept-2-ene"-4-alkynyl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt (molecular formula C
21H
25NHCl), beneficial microbe, soluble starch; The weight ratio of each composition is: (E)-N-(6,6-dimethyl hept-2-ene"-4-alkynyl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt 0.1～2%, beneficial microbe 0.1～4%, saccharide adjuvants 94～99.8% such as soluble starch, glucose.
The making of preparation of the present invention: with the abundant mix homogeneously of each component.When mixing, soluble starch must sieve by (100 order hole sizer), becomes fine powder.Terbinafine HCl is fully mixed with the soluble starch of equivalent, and the medicated powder behind the mix homogeneously mixes with the soluble starch of equivalent again, till medicated powder concentration reaches required concentration.Thereby reach even, the finely dispersed state of medicine of preparation granules size.
Preparation of the present invention can the prophylactic treatment bee fungus disease, also can be in order to the control of silkworm fungal disease.The present invention provides that a kind of (6,6-dimethyl hept-2-ene"-4-alkynyl)-(its molecular formula is C to N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt with (E)-N-
21H
25NHCl), the preparation of probiotic bacteria and saccharide adjuvant.(E)-N-(6,6-dimethyl hept-2-ene"-4-alkynyl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt is a kind of propylene amine drug with broad-spectrum antifungal activity.It can disturb the early stage biosynthesis of fungus ergosterol specifically, and highly selective suppresses the Squalene Cycloxygenase of fungus, the Squalene epoxidation reaction is obstructed, thereby reaches the effect of killing or suppressing fungus.Probiotic bacteria such as bacillus subtilis, Bacillus licheniformis can be kept young honeybee intestinal microbial population balance, optimizes function of intestinal canal, can replenish various nutrient substance simultaneously, and the ability raise immunity, reduces the probability of falling ill.Its culture contains the nutrient substance behind many probiotics fermentions, is easy to young honeybee and digests and assimilates, and the part tunning also has immunoloregulation function.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail.These embodiment are in order to demonstrate the invention, but not limit scope of the present invention by any way.
Material source: (E)-(6,6-dimethyl hept-2-ene"-4-alkynyl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt is available from platinum source, Shandong pharmaceutcal corporation, Ltd for N-, and purity is higher than 99.82%, powder.Separate bacillus subtilis and the bacillus licheniformis soil around the Nidus Vespae and obtain.The Apis bacillus bifidus, bifidobacterium asteroides or Bifidobacterium minimum or bifidobacterium coryneforme, Lactobacillus Jensenii and food starch milk bacillus and Lactobacillus plantarum and small lactobacillus, two Lactobacillus fermentis are separation and Culture gained in the Apis body all.Soluble starch and fructose, glucose are commercially available high purity product.
Embodiment 1:
Get (E)-N-(6,6-dimethyl hept-2-ene"-4-alkynyl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt 0.5 gram, bacillus subtilis 1 gram, viable count is 2 * 10
9Individual/gram, soluble starch 98.5 grams fully are Powdered behind the mix homogeneously.
Embodiment 2:
Get (E)-N-(6,6-dimethyl hept-2-ene"-4-alkynyl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt 1 gram, bacillus licheniformis 3 grams, viable count is 10
9Individual/gram, glucose 96 grams, fully mix homogeneously is Powdered.
Embodiment 3:
Get (E)-N-(6,6-dimethyl hept-2-ene"-4-alkynyl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt 0.1 gram, Apis bacillus bifidus and bacillus licheniformis mixed vaccine 4 grams, viable count is 3 * 10
9Individual/gram, fructose 95.9 grams, fully mix homogeneously is Powdered.
Embodiment 4:
Get (E)-N-(6,6-dimethyl hept-2-ene"-4-alkynyl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt 2 grams, bifidobacterium asteroides or Bifidobacterium minimum or bifidobacterium coryneforme 0.1 gram, viable count is 5 * 10
9Individual/gram, glucose 97.9 grams, fully mix homogeneously is Powdered.
Embodiment 5:
Get (E)-N-(6,6-dimethyl hept-2-ene"-4-alkynyl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt 2 grams, Lactobacillus Jensenii and food starch milk bacillus and Lactobacillus plantarum and small lactobacillus mixed vaccine 4 grams, viable count is 4 * 10
9Individual/gram starch 94 grams, fully mix homogeneously is Powdered.
Embodiment 6:
Get (E)-N-(6,6-dimethyl hept-2-ene"-4-alkynyl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt 1 gram, bacillus subtilis and two Lactobacillus fermenti mixture 1 grams, viable count is 1.5 * 10
9Individual/gram, fructose 98 grams, fully mix homogeneously is Powdered.
The operation instruction of above-mentioned 6 routine pharmaceutical products:
The prevention time spent, it is soluble in water with preparation to restrain the ratio that agent is dissolved in 500 ml waters in 1, directly spray or the young honeybee of feeding on one's body; The treatment time spent, powder preparation directly is sprinkled upon body surface of disease honeybee and Apis body surface on every side.
(1) this preparation using dosage is few, and powder looses must be dead to the sick pathogenic bacteria of part Chalk, suffered from the sick young honeybee of Chalk, uses this preparation directly to be dispersed on one's body the ill young honeybee, reaches the effect of killing the sick pathogenic bacteria of Chalk, thereby prevent diffusion.
(2) suffer from the sick same a slice Nidus Vespae (Nidus Vespae) of Chalk and the young honeybee of other Nidus Vespaes in the same beehive and fall ill probably, but being incubation period of symptom do not occur.This kind Nidus Vespae dosage is identical with ill young honeybee, to reach the purpose of the young honeybee of protection.
(3) the sick spore of Chalk is a lot of in the surrounding, and (be young honeybee on one's body) breeding easily constantly spreads then in certain temperature, humidity and nutritious place.Therefore should prevent overweighting controlling, spread pesticides in a week 1-2 time comparatively suitable.Can make bee farm not have the Chalk disease, especially in season in spring and autumn.
(4) this preparation is soluble in water, spraying is perhaps fed also can reach the effect of control.
(5) preparation lucifuge is preserved below 30 ℃, and the shelf-life is 2 years.
Production of the present invention is convenient, and is with low cost, uses very conveniently, passes through beekeeper's application, and effect is obvious, receives very much bee keeper's welcome.
Above-mentioned 6 routine products are respectively applied for ascospherosis of bee and the sick result of use of silkworm muscardine compares:
1. prevention:
(1) ascospherosis of bee: 6 routine product preventive effects are followed successively by: routine 1:96%; Example 2:99%; Example 3:84%; Example 4:100%; Example 5:100%; Example 6:99%.
(2) the silkworm muscardine is sick: 6 routine product preventive effects are respectively: routine 1:95%; Example 2:98%; Example 3:85%; Example 4:99%; Example 5:100%; Example 6:98%.
2. treatment:
(1) ascospherosis of bee: 6 routine product therapeutic effect are respectively: routine 1:93%; Example 2:95%; Example 3:80%; Example 4:96%; Example 5:97%; Example 6:96%.
(2) the silkworm muscardine is sick: 6 routine product therapeutic effect are respectively: routine 1:92%; Example 2:95%; Example 3:81%; Example 4:98%; Example 5:99%; Example 6:97%.
Generally speaking, in spring, autumn, in the period that Chalk is sick occurred frequently, also do not occur using this preparation preventive effect very outstanding in the sick Nidus Vespae of Chalk.Treatment mainly is to rely on to prevent mycotic diffusion, play therapeutical effect, and the young honeybee of having fallen ill is cureless, will certainly die undoubtedly.Near the effect highly significant that young honeybee also that infect or low-grade infection is fallen ill and controls preventing.Therefore, anti-the overweighting of the use that this preparation is sick for Chalk, muscardine is sick controlled.
On the cost: this preparation price with (E)-N-(6,6-dimethyl hept-2-ene"-4-alkynyl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt, beneficial microorganism content just concern greatlyyer, the increase cost of content also can increase.With respect to prior art, effective ingredient ratio of the present invention is low, and processing technology is simple, and labour cost is few, and can hang down to drop into and realize high production and high service efficiency, be a very practical invention.
Claims (5)
1. preparation of preventing and treating bee fungus disease is characterized in that: by (E)-N-(6,6-dimethyl hept-2-ene"-4-yl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt, beneficial microbe and soluble starch or saccharide are formed.
2. according to the preparation of the said control bee fungus disease of claim 1; It is characterized in that: the weight ratio of each component is: (E)-and N-(6; 6-dimethyl hept-2-ene"-4-alkynyl)-N-methyl isophthalic acid-naphthalene methylamine hydrochloric salt 0.1～2%; Beneficial microbe 0.1～4%, soluble starch or saccharide 94～99.8%.
3. according to the preparation of the said control bee fungus disease of claim 1, it is characterized in that: said beneficial microbe is: bacillus subtilis, or Bacillus licheniformis, or Apis bacillus bifidus; Or bifidobacterium asteroides, or Bifidobacterium minimum, or bifidobacterium coryneforme; Or Lactobacillus Jensenii, or food starch milk bacillus, or Lactobacillus plantarum; Or small lactobacillus, or two Lactobacillus fermenti, or the combination in any of above-mentioned bacillus; Containing viable count in the said beneficial microbe 0.1～4% is 10
9～5 * 10
9Individual/gram.
4. according to the preparation of the said control bee fungus disease of claim 1, it is characterized in that: said saccharide is glucose or fructose.
5. be applied to the control of Apis or silkworm fungal disease according to the preparation of the said control bee fungus disease of claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101341023A CN102670664A (en) | 2012-04-28 | 2012-04-28 | Preparation for preventing and controlling bee fungal diseases |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101341023A CN102670664A (en) | 2012-04-28 | 2012-04-28 | Preparation for preventing and controlling bee fungal diseases |
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| CN102670664A true CN102670664A (en) | 2012-09-19 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109055268A (en) * | 2018-08-21 | 2018-12-21 | 云南农业大学 | A kind of compound micro-ecological preparation and its application during bee raising |
| CN109182165A (en) * | 2018-08-21 | 2019-01-11 | 云南农业大学 | One plant of Lactobacillus helveticus and its application during bee raising |
-
2012
- 2012-04-28 CN CN2012101341023A patent/CN102670664A/en active Pending
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109055268A (en) * | 2018-08-21 | 2018-12-21 | 云南农业大学 | A kind of compound micro-ecological preparation and its application during bee raising |
| CN109182165A (en) * | 2018-08-21 | 2019-01-11 | 云南农业大学 | One plant of Lactobacillus helveticus and its application during bee raising |
| CN109182165B (en) * | 2018-08-21 | 2021-08-31 | 云南农业大学 | Lactobacillus helveticus strain and application thereof in bee breeding process |
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Application publication date: 20120919 |