CN102670320A - Modeling method of jawbone osteoporosis in-vitro model and vacuum decalcification auxiliary device thereof - Google Patents
Modeling method of jawbone osteoporosis in-vitro model and vacuum decalcification auxiliary device thereof Download PDFInfo
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- CN102670320A CN102670320A CN2012101582960A CN201210158296A CN102670320A CN 102670320 A CN102670320 A CN 102670320A CN 2012101582960 A CN2012101582960 A CN 2012101582960A CN 201210158296 A CN201210158296 A CN 201210158296A CN 102670320 A CN102670320 A CN 102670320A
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Abstract
The invention belongs to maxillofacial surgery, relates to the field of basic research of the oral implantology of alveolar surgery and the matched machinery, and discloses a modeling method of a jawbone osteoporosis in-vitro model and a vacuum decalcification auxiliary device thereof. In the modeling method of a jawbone osteoporosis in-vitro model, a vacuum reaction environment is provided by use of a vacuum decalcification auxiliary device, so that the decalcification liquid is in contact with the jawbone outer cortical bone and inner cortical bone at the same time in the largest range to ensure synchronous decalcification of the inner and outer sclerotin of a jawbone specimen; and meanwhile, by controlling the reaction time, jawbone osteoporosis in-vitro models with different bone mineral densities can be quickly and controllably established.
Description
Technical field
The invention belongs to decorative sursery, relate to basic research and supporting instrument field, the especially modeling method of jawbone osteoporosis external model and the vacuum decalcification auxiliary device thereof of alveolus surgery oral implantology.
Background technology
Since nineteen sixty-five Sweden professor Branemark accomplished the first routine Dental Implant reparation in the world and obtains good clinical effectiveness, modern tooth-planting technology constantly moved to maturity, and was called as " the 3rd human secondary tooth ".At present, tooth implant is the best permanent repair mode of absence of tooth that medical circle of oral cavity is generally acknowledged.Discover that the successful key of plantation is the primary stability and synosteosis at a specified future date after implantation body implants, osteoporosis, the bone quantity not sufficient all can reduce primary stability and implantation body's synosteosis, thereby causes the plantation failure.Yet,, therefore, merit attention more to this type crowd's plantation treatment clinically because the case that absence of tooth need carry out Implant Prosthesis concentrates on more than 60 years old mostly, and this part patient usually is accompanied by this type of osteoporosis bone metabolic disease.
In order to improve the nearly long-term effect of Implant Prosthesis under the osteoporosis condition, way commonly used clinically at present comprises implantation body's configuration design, bone extrusion technique etc.Yet; Clinical preceding experimentation to various novel implantation bodys and bone extrusion technique; Owing to obtain relatively difficulty of osteoporosis BIAO and BEN; Be limited to mostly on the animal of normal sclerotin or healthy adult's jawbone and study less application osteoporosis or serious osteoporotic animal or human's class jawbone.Therefore, the osteoporosis model of setting up applied biomechanics research is to carry out basis of experimental before various novel tooth-plantings technology clinical.
Discover: the reduction of the bone density of sclerotin, bone strength and bone biomechanical property all has very big relation with the change of bone mineral nitrogen, bone matrix.And at present about setting up the research method of osteoporosis animal model; All be through disturbing intravital bone metabolism, finally cause bone-loss, causing osteoporosis; Yet; The method of the interior modeling of body all needs the time more than at least 6 months could form the osteoporosis animal model of operability, has limited and has carried out zooperal number of times, has prolonged search time.Therefore, the modeling method of a kind of jawbone of osteoporosis fast and effectively external model of searching becomes the direction that numerous research workers make great efforts.
At present, discover through method and also can cause its bone density, bone strength and bone biomechanical property to descend the sclerotin decalcification.Yet there is following shortcoming in this method: 1. osteoporosis degree poor controllability; 2. whole decalcification process is manual operations fully, and error is bigger, and it is controlled to set up the osteoporosis degree, accurately mechanical model; 3. the cortical bone of jawbone and spongy bone density variation are bigger, and outer fine and close cortical bone has hindered the reaction of decalcifying Fluid to the internal layer spongy bone, can not reach synchronous decalcification.Therefore, how can set up the controlled and effective osteoporosis jawbone external model of sclerotin rarefaction in the analogue body of osteoporosis degree, become the problem that the osteoporosis biomechanics Research waits to solve.
Summary of the invention
Defective or deficiency to above-mentioned prior art existence; The object of the invention is to provide a kind of modeling method of osteoporosis jawbone external model; This method can be set up the path of exposed inner spongy bone on jawbone; Utilize the negative pressure of vacuum environment, make the jawbone body when being soaked in decalcifying Fluid, can reach the effect of jawbone body spongy bone, the inside and outside decalcification synchronously of cortical bone.
Another object of the present invention is to provide a kind of vacuum decalcification auxiliary device, is used for the modeling method of osteoporosis jawbone external model.
In order to achieve the above object, the present invention takes following technical scheme to be achieved.
Technical scheme one:
A kind of modeling method of osteoporosis jawbone external model is characterized in that, may further comprise the steps:
(1) jawbone pretreatment and punching: the jawbone BIAO and BEN is divided into two by the place of associating, center, removes surperficial soft tissue, pull out all teeth, drill through more than one hole in jawbone lower edge bottom;
(2) evacuation pretreatment: remove skeletal detritus, surperficial oils and fats on the jawbone BIAO and BEN;
(3) evacuation: the jawbone BIAO and BEN is placed on evacuation in the closed reaction vessel;
(4) decalcification: in closed reaction vessel, feed decalcifying Fluid, and submergence jawbone BIAO and BEN;
(5) discharge opeing is cleaned: after the decalcification, discharge decalcifying Fluid or pull the jawbone BIAO and BEN out, clean.
The characteristics of technique scheme are with further improving:
In said step (1) jawbone pretreatment and the punching, punching is meant in jawbone lower edge bottom vertical punches.
In said step (2) the evacuation pretreatment, the jawbone BIAO and BEN is put into ultrasonator handle, remove its skeletal detritus, surperficial oils and fats.
In said step (4) decalcification, decalcifying Fluid is that mass fraction is 30% HCl.
In said step (1) jawbone pretreatment and the punching, punching is meant in jawbone lower edge bottom and is meant that use plantation section hole making drill vertically punches 1.5cm punching at interval, hole depth 0.5cm in jawbone lower edge bottom.Technical scheme two:
A kind of vacuum decalcification auxiliary device; Be used for the modeling method of osteoporosis jawbone external model, it is characterized in that, comprise closed reaction vessel; Its loam cake is provided with vacuum draw interface, separatory funnel interface; Said vacuum draw interface, separatory funnel interface are respectively arranged with valve, and said vacuum draw interface is connected with vacuum pump, and the separatory funnel interface is connected with separatory funnel.
The characteristics of technique scheme are with further improving:
The valve that the side of said closed reaction vessel or bottom surface are provided with leakage fluid dram and control its discharge opeing.
Said closed reaction vessel comprises loam cake and opening glass container, passes through rubber seal between said loam cake and the opening glass container.
The thickness of said opening glass container is 1cm, minister 25cm in it, wide 5cm, high 15cm.
The modeling method of osteoporosis jawbone external model of the present invention; Adopt the vacuum decalcification auxiliary device of design voluntarily; Be used on the jawbone of exodontia and equidistant punching; Cooperate negative pressure of vacuum, cortical bone, the spongy bone of jawbone can be tried one's best with decalcifying Fluid chemical reaction takes place fully in large area simultaneously, all occur the bone amount inside and outside the jawbone BIAO and BEN and reduce.Therefore, after this modeling method was handled, the structural change of osteoporosis appearance all appearred in the cortical bone of jawbone BIAO and BEN and spongy bone, to reach the purpose of the synchronous decalcification of sclerotin inside and outside the jawbone BIAO and BEN.
The present invention has following outstanding beneficial effect: the one, and vacuum pump has the advantage that can produce negative pressure; Outside the jawbone of handling, produce the negative pressure of vacuum environment through vacuum pump; Guarantee decalcifying Fluid with the maximum contact area, it is inner and surperficial to immerse jawbone simultaneously, in addition; Through adjustment decalcification time, the osteoporosis jawbone external model of the different bone mineral level of setting up that can be quick, controlled.The 2nd, decalcifying Fluid is under vacuum environment; The almost same time is through exodontia nest, the inner spongy bone of jawbone lower edge bottom hole contact jawbone; Outside contact surface cortical bone simultaneously; Spread step by step to the jawbone depths more afterwards, thereby make whole jawbone evenly decalcification synchronously occur, the model of setting up more is similar to the osteoporosis under the physiological status.The 3rd, adopt vacuum decalcification auxiliary device; Avoided can't decalcifying Fluid being injected into the situation in the jawbone BIAO and BEN fully because of manual operations; Guaranteed the bone loss of the inner spongy bone of jawbone; Reduced experimental error, improved the repeatability that osteoporosis model is set up, the osteoporosis jawbone external model of setting up the different bone mineral level for calibration provides possibility.
Description of drawings
Below in conjunction with the accompanying drawing and the specific embodiment the present invention is done further explain.
Fig. 1 is the structural representation of the vacuum decalcification auxiliary device of invention;
Fig. 2 is the local mounting structure sketch map of vacuum decalcification auxiliary device;
Fig. 3 is the elevational schematic view of the loam cake of closed reaction vessel;
Fig. 4 is jawbone pretreatment and punching process sketch map.
Wherein: 1, vacuum draw joint; 2, separatory funnel interface; 3, separatory funnel; 4, closed reaction vessel; 5, jawbone BIAO and BEN; 6, vacuum pump; 7, buckle; 8, vacuum draw interface; 9, groove; 10, leakage fluid dram.
The specific embodiment
Below in conjunction with accompanying drawing embodiments of the invention are described, embodiment is not construed as limiting the invention.
With reference to Fig. 1, Fig. 2, Fig. 3, be a kind of vacuum decalcification auxiliary device of the present invention, be used for the modeling method of osteoporosis jawbone external model.Wherein, Closed reaction vessel 4 has loam cake and opening glass container; Its loam cake is provided with vacuum draw interface 8, separatory funnel interface 2; Vacuum draw interface 8, separatory funnel interface 2 are respectively arranged with valve, and vacuum draw interface 8 connects vacuum pump 6 through vacuum draw joint 1, pipeline successively, and separatory funnel interface 2 connects separatory funnel 3.The valve that the side of closed reaction vessel 4 is provided with leakage fluid dram 10 and controls its discharge opeing, leakage fluid dram 10 also can be arranged on closed reaction vessel 4 bottom surfaces.
The thickness of opening glass container is 1cm, minister 25cm in it, wide 5cm, high 15cm.Loam cake be provided with groove 9 along portion, be placed with rubber blanket in the groove 9, clamping between the groove 9 of loam cake and the oral area of opening glass container.In addition, a side of loam cake and opening glass container is through the pivot pin active link, and corresponding side is provided with locking with buckle 7; Separatory funnel 3 carries an independent valve, guarantees when aspiration vacuum in the separatory funnel 3 that liquid or air can not penetrate in the closed reaction vessel.
Adopt above-mentioned vacuum decalcification auxiliary device, can implement the modeling method of osteoporosis jawbone external model, its concrete steps are following:
(1) jawbone pretreatment and punching: jawbone BIAO and BEN 5 is divided into two by the place of associating, center, removes surperficial soft tissue, pull out all teeth, drill through more than one hole, vertical jawbone lower edge bottom, this hole in jawbone lower edge bottom; Its mesopore interbody spacer is 1.5cm, hole depth 0.5cm.
Detailed process is as shown in Figure 4, and at first, the jawbone BIAO and BEN is divided into two by the place of associating, center, removes surperficial soft tissue; Secondly, pull out all teeth; At last, use tooth-planting section hole making drill vertically to punch in jawbone lower edge bottom, interval 1.5cm, hole depth 0.5cm its objective is to expose inner spongy bone.
(2) evacuation pretreatment: the jawbone BIAO and BEN is put into ultrasonator handle, remove its skeletal detritus, surperficial oils and fats, the processing time is about 30 minutes;
(3) evacuation: the jawbone BIAO and BEN is placed on evacuation in the closed reaction vessel; Be specially: close the valve of separatory funnel seam, the valve of open vacuum suction seam is communicated with vacuum pump through rubber tube; The open vacuum pump;, stop evacuation, and close the valve of vacuum draw seam during up to the pressure of vacuum pump suction QI KOU at least less than 0.1Pa.
(4) decalcification: add the 1000mL decalcifying Fluid in the separatory funnel, decalcifying Fluid is that mass fraction is 30% HCl; Then, open the valve of separatory funnel and separatory funnel seam successively, decalcifying Fluid feeds in the closed reaction vessel, and submergence jawbone BIAO and BEN, generally leaves standstill 30 minutes.Different if desired decalcification grades can be divided through time of repose (being the response time).
(5) discharge opeing is cleaned: after the decalcification, open the valve at leakage fluid dram place, discharge decalcifying Fluid, reuse washed with de-ionized water jawbone BIAO and BEN.
The modeling method of jawbone osteoporosis external model of the present invention; Utilize vacuum decalcification auxiliary device that a vacuum response environment is provided; Make contacting simultaneously of decalcifying Fluid maximum magnitude, with the synchronous decalcification of sclerotin inside and outside the assurance jawbone BIAO and BEN, simultaneously with jawbone outer cortex bone and internal layer spongy bone; Through control, can set up to fast controllable the osteoporosis jawbone external model of different bone mineral to the response time.More than show and described ultimate principle of the present invention, principal character and advantage of the present invention.Those skilled in the art should understand the present invention and not be restricted to the described embodiments; That describes in the foregoing description and the description just explains principle of the present invention; Under the prerequisite that does not break away from spirit and scope of the invention; The present invention also has various changes and modifications, and these variations and improvement all fall in the scope that requires protection.
Claims (10)
1. the modeling method of an osteoporosis jawbone external model is characterized in that, may further comprise the steps:
(1) jawbone pretreatment and punching: the jawbone BIAO and BEN is divided into two by the place of associating, center, removes surperficial soft tissue, pull out all teeth, drill through more than one hole in jawbone lower edge bottom;
(2) evacuation pretreatment: remove skeletal detritus, surperficial oils and fats on the jawbone BIAO and BEN;
(3) evacuation: the jawbone BIAO and BEN is placed on evacuation in the closed reaction vessel;
(4) decalcification: in closed reaction vessel, feed decalcifying Fluid, and submergence jawbone BIAO and BEN;
(5) discharge opeing is cleaned: after the decalcification, discharge decalcifying Fluid or pull the jawbone BIAO and BEN out, clean.
2. according to the modeling method of the said osteoporosis jawbone of claim 1 external model, it is characterized in that in said step (1) jawbone pretreatment and the punching, punching is meant in jawbone lower edge bottom vertical punches.
3. according to the modeling method of the said osteoporosis jawbone of claim 1 external model, it is characterized in that, in said step (2) the evacuation pretreatment, the jawbone BIAO and BEN is put into ultrasonator handle, remove its skeletal detritus, surperficial oils and fats.
4. according to the modeling method of the said osteoporosis jawbone of claim 1 external model, it is characterized in that in said step (4) decalcification, decalcifying Fluid is that mass fraction is 30% HCl.
5. according to the modeling method of the said osteoporosis jawbone of claim 1 external model; It is characterized in that in said step (1) jawbone pretreatment and the punching, punching is meant in jawbone lower edge bottom uses plantation section hole making drill vertically to punch; Interval 1.5cm, hole depth 0.5cm.
6. vacuum decalcification auxiliary device; Be used for the modeling method of the described osteoporosis jawbone of claim 1 external model, it is characterized in that, comprise closed reaction vessel; Its loam cake is provided with vacuum draw interface, separatory funnel interface; Said vacuum draw interface, separatory funnel interface are respectively arranged with valve, and said vacuum draw interface is connected with vacuum pump, and the separatory funnel interface is connected with separatory funnel.
7. vacuum decalcification auxiliary device according to claim 6 is characterized in that the valve that the side of said closed reaction vessel or bottom surface are provided with leakage fluid dram and control its discharge opeing.
8. vacuum decalcification auxiliary device according to claim 6 is characterized in that said closed reaction vessel comprises loam cake and opening glass container, passes through rubber seal between said loam cake and the opening glass container container.
9. vacuum decalcification auxiliary device according to claim 6 is characterized in that the thickness of said opening glass container is 1cm, minister 25cm in it, wide 5cm, high 15cm.
10. the modeling method of an osteoporosis jawbone external model based on the described vacuum decalcification of claim 6 auxiliary device, is characterized in that, may further comprise the steps:
(1) jawbone pretreatment and punching: the jawbone BIAO and BEN is divided into two by the place of associating, center, removes surperficial soft tissue, pull out all teeth, drill through more than one hole in jawbone lower edge bottom;
(2) evacuation pretreatment: remove skeletal detritus, surperficial oils and fats on the jawbone BIAO and BEN;
(3) evacuation: the jawbone BIAO and BEN is placed on evacuation in the closed reaction vessel;
(4) decalcification: in closed reaction vessel, feed decalcifying Fluid, and submergence jawbone BIAO and BEN;
(5) discharge opeing is cleaned: after the decalcification, discharge decalcifying Fluid or pull the jawbone BIAO and BEN out, clean.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210158296.0A CN102670320B (en) | 2012-05-21 | 2012-05-21 | Modeling method of jawbone osteoporosis in-vitro model and vacuum decalcification auxiliary device thereof |
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| CN201210158296.0A CN102670320B (en) | 2012-05-21 | 2012-05-21 | Modeling method of jawbone osteoporosis in-vitro model and vacuum decalcification auxiliary device thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107664591A (en) * | 2016-07-30 | 2018-02-06 | 中国科学院近代物理研究所 | The device and its application method that a kind of analog bone ore deposit is lost in |
| CN108717006A (en) * | 2018-08-14 | 2018-10-30 | 宁波江丰生物信息技术有限公司 | A kind of quick decalcification instrument and its method |
| CN111871349A (en) * | 2020-07-24 | 2020-11-03 | 湖北省农业科学院农产品加工与核农技术研究所 | Pretreatment decalcification method for preparing peptide from fish scales |
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| CN1569249A (en) * | 2003-07-11 | 2005-01-26 | 中国人民解放军第一军医大学基础部 | Preparation of bone transplantation material by formaldehyde fixed homolotous variant bone |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1569249A (en) * | 2003-07-11 | 2005-01-26 | 中国人民解放军第一军医大学基础部 | Preparation of bone transplantation material by formaldehyde fixed homolotous variant bone |
Non-Patent Citations (2)
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| 任长庆，童明超: "骨组织快速脱钙法", 《人民军医》, no. 3, 31 December 1986 (1986-12-31), pages 75 - 76 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107664591A (en) * | 2016-07-30 | 2018-02-06 | 中国科学院近代物理研究所 | The device and its application method that a kind of analog bone ore deposit is lost in |
| CN107664591B (en) * | 2016-07-30 | 2024-04-02 | 中国科学院近代物理研究所 | Device for simulating bone mineral loss and application method thereof |
| CN108717006A (en) * | 2018-08-14 | 2018-10-30 | 宁波江丰生物信息技术有限公司 | A kind of quick decalcification instrument and its method |
| CN111871349A (en) * | 2020-07-24 | 2020-11-03 | 湖北省农业科学院农产品加工与核农技术研究所 | Pretreatment decalcification method for preparing peptide from fish scales |
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