CN1569249A - Preparation of bone transplantation material by formaldehyde fixed homolotous variant bone - Google Patents
Preparation of bone transplantation material by formaldehyde fixed homolotous variant bone Download PDFInfo
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- CN1569249A CN1569249A CN 03139790 CN03139790A CN1569249A CN 1569249 A CN1569249 A CN 1569249A CN 03139790 CN03139790 CN 03139790 CN 03139790 A CN03139790 A CN 03139790A CN 1569249 A CN1569249 A CN 1569249A
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Abstract
The invention concerns tissue engineering, biomaterial, orthopaedics and tranmatology fields. The invention utilizes the homologuous bone after fixed by formaldehyde to process bone transplantation material for clinical bone defect rehabilitation. The preparation of the material mainly comprises drawing materials, removing formaldehyde, removing grease and protein, drying and disinfecting, which can be showed as follows: 1.drawing adult corpse bone specimen being fixed by 10% formalin over 6 months 2.flush trimming soft tissue 3.flushing bone tissue surface by water 4. cutting it to bone granules or bone strips 5.dipping them into deionized water for 24 hours 6. dipping to SS solution(mixture solution of 5%-15% sodium bisulphate and 1%-3%sodium hydroxide) for 48 hours 7.flushing by deionized water then dipping for 24 hours 8. dipping to 20% hydrogen peroxide solution, with temperature being 38 DEG C for 24 hours 9.removing filtered articles by centrifugation 10. conduct ultrasonic degreasing for 12 hours 11. digesting by 0.5% parenzyme for 12 hours, the temperature being 37 DEG C 12.centrifugating 13.repeat step 8 14.centrifugating 15. repeat step 10 16. vacuum drying 16. sterilizing by radiation 17.getting finished product.
Description
Technical field the present invention utilizes formaldehyde fixed bone processed to be the homogeneous allogenic bone transplantation material, relates to tissue engineering, biomaterial and orthopaedics and tranmstology field.
The reparation that the technical background bone is damaged is the constantly important topic of research of surgery worker for centuries always.Though autologous bone transplanting obtains general approval, bone is damaged does not on a large scale still have good substitute to be used for repairing and treatment.Present result of study shows that all the nature bone derived material has bone conduction and bone-inducting active preferably, and xenogenesis bone derived material obviously has stronger rejection than the allogeneic derived material, and homogeneous allogenic bone derived material and more close from the body bone, three-dimensional pore space structure with people's bone, and it is better bio-mechanical property and biocompatibility are arranged, and stronger on bone conduction and bone inducibility.But used allogeneic derived material all is to take from the fresh bones tissue to handle at present, and the source of material mainly is dead corpse, the limbs of unexpected amputation like this.But it is less relatively to originate, and can not guarantee the quality of osseous tissue, and pathophorous risk is arranged.
Formaldehyde is a kind of cheapness, effectively organizes anticorrosion fixative, and can kill pathogen completely and have antigenic effect.
Summary of the invention the present invention utilizes the suitable damaged homogeneous allogenic bone transplantation material of clinical repair bone of the bone processed after the formaldehyde fixed.
This material preparation mainly contains draws materials; Piptonychia aldehyde; ，：10％6——→——→——→——→24——→SS （ 5～15％1～3％ ） 48——→24——→20％38℃24——→——→12——→0.5％37℃12——→——→20％38℃24——→——→12——→0.5％37℃12——→——→——→——→。
The specific embodiment
(embodiment 1) is got bone specimen and is rejected soft tissue, with clear water that the osseous tissue surface washing is clean, osseous tissue is cut into the skeletal grain of 5 * 5 * 5mm, after putting into the deionized water immersion skeletal grain is put into SS solution (the mixed liquid of 5% sodium sulfite and 1% sodium hydroxide) immersion taking-up after 48 hours, with deionized water rinsing and immersion, soak skeletal grain with 20% hydrogenperoxide steam generator, centrifugal removal filtrate after the water-bath, ultrasonic fat, centrifugal behind the trypsinization skeletal grain, reuse 20% hydrogenperoxide steam generator soaks, centrifugal after the water-bath, ultrasonic fat, trypsinization are centrifugal again, irradiation sterilization behind the vacuum drying, bone is transplanted skeletal grain thereby the allogeneic that obtains outmoded bone source is derived.Do not make 2,4 dinitrophenyl hydrazine detect liquid by lixiviating solution and positive reaction occurs, and the contrast liquid that contains 2.2 μ g formaldehyde makes that after 12 hours detecting liquid positive reaction occurs the test experience result's of remaining formalin analysis display material.With the distilled water is blank liquid, and be 0 three times through the spectrophotometric determination absorbance after the colour developing of material lixiviating solution, illustrate that contained content of formaldehyde of this material and blank distilled water do not have the significance difference, this result of the test can illustrate that this novel bone grafting material does not contain formaldehyde substantially.
(embodiment 2) get bone specimen, reject soft tissue, with clear water that the osseous tissue surface washing is clean, osseous tissue is cut into the bone bar of 5 * 5 * 40mm, put into and to put into SS solution (the mixed liquid of 15% sodium sulfite and 3% sodium hydroxide) immersion taking-up after 48 hours after deionized water soaks, with deionized water rinsing and immersion, soak skeletal grain with 20% hydrogenperoxide steam generator, centrifugal removal filtrate after the water-bath, ultrasonic fat is handled, and is centrifugal behind the trypsinization skeletal grain, and reuse 20% hydrogenperoxide steam generator soaks, centrifugal after the water-bath, ultrasonic fat, centrifugal again behind the trypsinization, vacuum drying, irradiation sterilization, the allogeneic that the obtains outmoded bone source bone of deriving is transplanted skeletal grain.Do not make 2,4 dinitrophenyl hydrazine detect liquid by lixiviating solution and positive reaction occurs, and the contrast liquid that contains 2.2 μ g formaldehyde makes that after 12 hours detecting liquid positive reaction occurs the test experience result's of remaining formalin analysis display material.With the distilled water is blank liquid, and be 0 three times through the spectrophotometric determination absorbance after the colour developing of material lixiviating solution, illustrate that contained content of formaldehyde of this material and blank distilled water do not have the significance difference, this result of the test can illustrate that this novel bone grafting material does not contain formaldehyde substantially.
Claims (3)
1, a kind of novel bone defect repair graft materials is made packed products behind piptonychia aldehyde, defat, deproteinization and irradiation sterilization by the people's homogeneous allogenic bone through the anticorrosion mistake of formalin.
2, prepare of method and the technological process of Patent right requirement 1 described novel bone defect repair with the homogeneous allogenic bone transplantation material.
3, require 1,2 to experimentize and detect resulting related data data according to patent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03139790 CN1569249A (en) | 2003-07-11 | 2003-07-11 | Preparation of bone transplantation material by formaldehyde fixed homolotous variant bone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03139790 CN1569249A (en) | 2003-07-11 | 2003-07-11 | Preparation of bone transplantation material by formaldehyde fixed homolotous variant bone |
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| Publication Number | Publication Date |
|---|---|
| CN1569249A true CN1569249A (en) | 2005-01-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 03139790 Pending CN1569249A (en) | 2003-07-11 | 2003-07-11 | Preparation of bone transplantation material by formaldehyde fixed homolotous variant bone |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2431408A (en) * | 2005-10-19 | 2007-04-25 | Osta Technologies Cc | Bone material and a process for the preparation of bone material |
| CN100389653C (en) * | 2005-02-06 | 2008-05-28 | 杨成杰 | Manufacturing technique of biomass full body bone structural connection blood vessel casting mould specimen |
| CN100415308C (en) * | 2006-09-12 | 2008-09-03 | 南方医科大学 | A new type of homologous bone and its preparation method |
| CN100430096C (en) * | 2006-03-16 | 2008-11-05 | 王立飞 | Method for continuous hypothermal desiccation and sterilization of bony tissue, and equipment therefor |
| US7998206B2 (en) * | 2005-03-09 | 2011-08-16 | Arthrex, Inc. | Osteochondral core centrifugation system and method of osteochondral repair |
| CN102670320A (en) * | 2012-05-21 | 2012-09-19 | 中国人民解放军第四军医大学 | Modeling method of jawbone osteoporosis in-vitro model and vacuum decalcification auxiliary device thereof |
| CN107583108A (en) * | 2017-10-31 | 2018-01-16 | 陕西爱骨医疗股份有限公司 | A kind of preparation method of bone grafting material |
| CN111213632A (en) * | 2019-11-19 | 2020-06-02 | 长春中医药大学 | Method and equipment for making animal medicine epoxy resin specimen |
-
2003
- 2003-07-11 CN CN 03139790 patent/CN1569249A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100389653C (en) * | 2005-02-06 | 2008-05-28 | 杨成杰 | Manufacturing technique of biomass full body bone structural connection blood vessel casting mould specimen |
| US7998206B2 (en) * | 2005-03-09 | 2011-08-16 | Arthrex, Inc. | Osteochondral core centrifugation system and method of osteochondral repair |
| GB2431408A (en) * | 2005-10-19 | 2007-04-25 | Osta Technologies Cc | Bone material and a process for the preparation of bone material |
| CN100430096C (en) * | 2006-03-16 | 2008-11-05 | 王立飞 | Method for continuous hypothermal desiccation and sterilization of bony tissue, and equipment therefor |
| CN100415308C (en) * | 2006-09-12 | 2008-09-03 | 南方医科大学 | A new type of homologous bone and its preparation method |
| CN102670320A (en) * | 2012-05-21 | 2012-09-19 | 中国人民解放军第四军医大学 | Modeling method of jawbone osteoporosis in-vitro model and vacuum decalcification auxiliary device thereof |
| CN102670320B (en) * | 2012-05-21 | 2014-12-17 | 中国人民解放军第四军医大学 | Modeling method of jawbone osteoporosis in-vitro model and vacuum decalcification auxiliary device thereof |
| CN107583108A (en) * | 2017-10-31 | 2018-01-16 | 陕西爱骨医疗股份有限公司 | A kind of preparation method of bone grafting material |
| CN111213632A (en) * | 2019-11-19 | 2020-06-02 | 长春中医药大学 | Method and equipment for making animal medicine epoxy resin specimen |
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