CN108717006A - A kind of quick decalcification instrument and its method - Google Patents
A kind of quick decalcification instrument and its method Download PDFInfo
- Publication number
- CN108717006A CN108717006A CN201810922736.2A CN201810922736A CN108717006A CN 108717006 A CN108717006 A CN 108717006A CN 201810922736 A CN201810922736 A CN 201810922736A CN 108717006 A CN108717006 A CN 108717006A
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- Prior art keywords
- decalcification
- electrolytic cell
- quick
- bone tissue
- liquid
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 77
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 39
- 239000002699 waste material Substances 0.000 claims abstract description 37
- 210000000988 bone and bone Anatomy 0.000 claims abstract description 36
- 238000005868 electrolysis reaction Methods 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 239000012530 fluid Substances 0.000 claims description 14
- 229910052791 calcium Inorganic materials 0.000 claims description 6
- 239000011575 calcium Substances 0.000 claims description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- 239000004973 liquid crystal related substance Substances 0.000 claims description 3
- 230000003993 interaction Effects 0.000 claims description 2
- 108010066114 cabin-2 Proteins 0.000 description 11
- 230000008569 process Effects 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
- 238000013139 quantization Methods 0.000 description 3
- 244000299461 Theobroma cacao Species 0.000 description 2
- 235000009470 Theobroma cacao Nutrition 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000018678 bone mineralization Effects 0.000 description 1
- 230000002308 calcification Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 238000011532 immunohistochemical staining Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
Abstract
The present invention provides a kind of quick decalcification instrument, including main body, reagent trough and control system, wherein：The sample cabin that the main body includes electrolytic cell, is set in the electrolytic cell；The electrolytic cell is connect by inlet/outlet pipe with the reagent trough；The sample cabin is connected to the electrolytic cell, for placing the bone tissue for waiting for decalcification；The reagent trough is connect with the electrolytic cell of the main body, for providing the reagent needed for decalcification and holding the waste liquid of decalcification discharge；And reagent is inputted electrolytic cell by the control system for controlling from the reagent trough, and decalcification is carried out to the bone tissue in sample cabin.The present invention also provides a kind of decalcification methods of above-mentioned quick decalcification instrument.The quick decalcification instrument and its method of the present invention, can realize quick decalcification, and will not cause to damage to bone tissue.
Description
Technical field
The present invention relates to a kind of decalcification instrument more particularly to a kind of quick decalcification instrument and its methods.
Background technology
When Pathological experiment makes and is sliced, through being commonly encountered some calcic tissues, and calcium is very hard.Due in tissue
Density between calcium and paraffin is different, and the tissue of calcic generally cannot directly make slice.Bone tissue calcium content is most.In addition to bone
Calcification can also occur for other tissues except tissue, calcification area be formed in the tissue, so needing by decalcification process.Decalcification is system
The important link for making bone tissue slice, will especially carry out the bone tissue of immunohistochemical staining.The major function of decalcification instrument
It is for decalcification.
Currently, traditional decalcification mode has as a drawback that：1, decalcification time is long；2, decalcification process is not environmentally；3, decalcification
Process may can cause centainly to damage to bone tissue.
Invention content
The technical problem to be solved by the present invention is to：In view of the drawbacks of the prior art, provide a kind of quick decalcification instrument and its
Method can realize quick decalcification, and will not cause to damage to bone tissue.
The present invention uses following technical scheme to solve above-mentioned technical problem：
It is an object of the present invention to provide a kind of quick decalcification instrument, including main body, reagent trough and control system, wherein：
The sample cabin that the main body includes electrolytic cell, is set in the electrolytic cell；The electrolytic cell by inlet/outlet pipe with
The reagent trough connection；The sample cabin is connected to the electrolytic cell, for placing the bone tissue for waiting for decalcification；
The reagent trough is connect with the electrolytic cell of the main body, for providing the reagent needed for decalcification and holding decalcification discharge
Waste liquid；And
Reagent is inputted electrolytic cell by the control system for controlling from the reagent trough, and to the bone tissue in sample cabin
Carry out decalcification.
In order to optimize above-mentioned technical solution, the technical measures that the present invention takes further include：
Further, the reagent trough includes decalcification liquid bath, Rinsing Area and waste liquid tank, and the inlet/outlet pipe includes for that will take off
Calcium liquid is passed through the decalcification liquid pipe of the electrolytic cell from the decalcification liquid bath, for clear water to be passed through the electrolysis from the Rinsing Area
The water pipe of slot, the sewer pipe for waste liquid to be passed through to the waste liquid tank from the electrolytic cell, the decalcification liquid pipe, water pipe and
Pump is separately installed on sewer pipe.
Further, the liquid level sensor being connect with the control system is equipped in the electrolytic cell；Liquid level sensor is used
Height of liquid level in induction electrolytic cell, in order to which control system is according to the work of the Liquid level pump in electrolytic cell.More into one
Step ground, there are two the liquid level sensors, is respectively arranged at the bottom and default upper liquid level line of the electrolytic cell madial wall.
Further, several through-holes are offered on the wall in the sample cabin, for the sample cabin and the electrolytic cell
Between connection.The decalcifying Fluid or clear water for inputting electrolytic cell can be entered by the through-hole in sample cabin, and then realize that electrolysis is de-
Calcium or aquation；Waste liquid in sample cabin can enter electrolytic cell by the through-hole, and then will be useless in sample cabin and in electrolytic cell
Liquid is expelled to reagent trough.
Further, the main body further includes shell, and the shell is used for people equipped with what is connect with the control system
Button, indicator light and the liquid crystal display of machine interaction.
Further, the control system control bone tissue progress decalcification includes the control to the decalcification working time and right
The control of decalcification electrolysis strength.Further, the decalcification working time can be adjusted according to demand, be set as the time choosing of 1h-12h
It selects；Decalcification electrolysis strength cocoa is adjusted according to demand, is set as 7 grades of intensity selections.
Further, the quick decalcification instrument further includes the power supply for being set to the body interior, and the power supply includes electricity
Source adapter and rechargeable battery；The power supply adaptor is used to external reference voltages output be the suitable quick decalcification instrument work
The operating voltage of work includes being for the first power supply adaptor of suitable pump work, by external voltage output by external voltage output
It is suitble to the second source adapter of control system work.
It is a further object to provide a kind of method being electrolysed decalcification using the quick decalcification instrument, including it is following
Step：
S1, decalcifying Fluid is passed through electrolytic cell from reagent trough；
S2, electrolysis decalcification is carried out to bone tissue；
S3, the waste liquid after electrolysis decalcification is drained into reagent trough from electrolytic cell.
Further, S1-S3 can repeatedly be recycled according to electrolysis decalcification demand.
It is a further object to provide a kind of methods using the quick decalcification instrument aquation, after S3, packet
Include following steps：
S4, clear water is passed through electrolytic cell from reagent trough；
S5, aquation is carried out to the bone tissue after electrolysis decalcification；
S6, the waste liquid after aquation is drained into reagent trough from electrolytic cell；
S7, the bone tissue for obtaining decalcification completion.
Further, S4-S6 can repeatedly be recycled according to aquation demand.
The present invention is had the following technical effect that compared with prior art using above-mentioned technical proposal：
1) quick decalcification instrument of the invention not only greatly accelerates decalcification speed, also using decalcifying Fluid cooperation electrolysis decalcification
Bone tissue will not be caused to damage.
2) using electrolysis two steps of decalcification and aquation so that entire decalcification process environmental protection of the invention.
3) quick decalcification instrument of the invention can be by decalcification course quantization, and simultaneously decalcification time is arranged in adjustable decalcification intensity,
Suitable for various bone tissue decalcifications.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram of preferred embodiment of the quick decalcification instrument of the present invention；
Fig. 2 is a kind of module diagram of preferred embodiment of the quick decalcification instrument of the present invention；
Fig. 3 is a kind of flow chart of preferred embodiment of the decalcification method of the quick decalcification instrument of the present invention；
Wherein reference numeral is：
1- electrolytic cells；2- samples cabin；3- master control borads；4- decalcification liquid baths；5- Rinsing Areas；6- waste liquid tanks；7- decalcification liquid pumps；8-
Clarified water pump；9- waste drains pumps；10- decalcification liquid pipes；11- water pipes；12- sewer pipes.
Specific implementation mode
Technical scheme of the present invention is described in further detail with embodiment below in conjunction with the accompanying drawings.Following embodiment is only
For clearly illustrating technical scheme of the present invention, and not intended to limit the protection scope of the present invention.
Embodiment 1
The present embodiment provides a kind of quick decalcification instrument.
Referring to Fig. 1 and Fig. 2, a kind of quick decalcification instrument, including main body, reagent trough and control system, wherein：
The sample cabin 2 that main body includes electrolytic cell 1, is set in electrolytic cell 1；Electrolytic cell 1 is connected by inlet/outlet pipe and reagent trough
It connects；Sample cabin 2 is connected to electrolytic cell 1, for placing the bone tissue for waiting for decalcification；Reagent trough and the electrolytic cell of main body 1 connect, and are used for
Reagent needed for decalcification is provided and holds the waste liquid of decalcification discharge；Control system is electrolysed for controlling to input reagent from reagent trough
Slot 1, and decalcification is carried out to the bone tissue in sample cabin 2.
In the present embodiment, by main body, reagent trough with and control system cooperation：Main body provides the field of bone tissue decalcification
Institute；Reagent trough is provided for being electrolysed the decalcifying Fluid of decalcification, for the clear water of aquation, and to provide the place of discharging of waste liquid；Control
System includes master control borad 3, and the setting of master control borad 3 is in body interior, for controlling electrolysis decalcification and hydration process；So as to realize
Quick decalcification, environmentally friendly decalcification, and bone tissue will not be caused to damage.In the present embodiment, after decalcifying Fluid input electrolytic cell 1,
Since electrolytic cell 1 is connected to sample cabin 2, decalcifying Fluid can enter sample cabin 2 from electrolytic cell 1, and bone tissue is put into sample cabin 2
Afterwards, power on and electrolysis decalcification can be achieved.
In a preferred embodiment, reagent trough includes decalcification liquid bath 4, Rinsing Area 5 and waste liquid tank 6, and inlet/outlet pipe includes being used for
Decalcifying Fluid is passed through the decalcification liquid pipe 10 of electrolytic cell 1, for clear water to be passed through the clear of electrolytic cell 1 from Rinsing Area 5 from decalcification liquid bath 4
Water pipe 11, the sewer pipe 12 for waste liquid to be passed through to waste liquid tank 6 from electrolytic cell 1, decalcification liquid pipe 10, water pipe 11 and sewer pipe 12
On be separately installed with pump.Decalcification liquid pump 7 in decalcification liquid pipe 10, is mounted on the clarified water pump 8 in water pipe 11
Waste drains pump 9 on sewer pipe 12 is connect with control system respectively.
The liquid level sensor being connect with control system is equipped in electrolytic cell 1；Liquid level sensor is for incuding in electrolytic cell 1
Height of liquid level, in order to which control system is according to the work of the Liquid level pump in electrolytic cell 1.There are two liquid level sensors, respectively
It is set at the bottom and default upper liquid level line of 1 madial wall of electrolytic cell.
In the present embodiment, when starting to be electrolysed decalcification, control system controls decalcification liquid pump 7 by decalcifying Fluid from decalcification liquid bath
4 are passed through in electrolytic cell 1, when the liquid level sensor at default upper liquid level line senses that the liquid level in electrolytic cell 1 reaches default
When value, control system control decalcification liquid pump 7 is stopped；When being electrolysed decalcification completion, control system controls waste drains pump 9 and will be electrolysed
Waste liquid after decalcification is expelled to from electrolytic cell 1 in waste liquid tank 6, when the liquid level sensor at bottom senses in electrolytic cell 1
Waste liquid it is drained when, control system control waste drains pump 9 be stopped；When starting aquation, control system controls clarified water pump 8 will be clear
Water is passed through from Rinsing Area 5 in electrolytic cell 1, when the liquid level sensor at default upper liquid level line senses the liquid in electrolytic cell 1
When position reaches preset value, control system control clarified water pump 8 is stopped；When aquation is completed, control system controls waste drains pump 9 will
Waste liquid after aquation is expelled to from electrolytic cell 1 in waste liquid tank 6, when the liquid level sensor at bottom senses in electrolytic cell 1
Waste liquid it is drained when, control system control waste drains pump 9 be stopped.To realize automation mechanized operation.
In a preferred embodiment, several through-holes are offered on the wall in sample cabin 2, for sample cabin 2 and electrolytic cell 1 it
Between connection.Input electrolytic cell 1 decalcifying Fluid or clear water can be entered by through-hole in sample cabin 2, and then realize electrolysis decalcification or
Aquation；Waste liquid in sample cabin 2 can enter electrolytic cell 1 by through-hole, and then the waste liquid in sample cabin 2 and in electrolytic cell 1 is equal
It is expelled to reagent trough.
In a preferred embodiment, main body further includes shell, and shell is used for man-machine friendship equipped with what is connect with control system
Mutual button, indicator light and liquid crystal display.It is user-friendly.
In a preferred embodiment, control system control bone tissue carries out decalcification, includes the control to the decalcification working time
With the control to decalcification electrolysis strength.The decalcification working time can be adjusted according to demand, be set as the selection of time of 1h-12h；Decalcification
Electrolysis strength cocoa is adjusted according to demand, is set as 7 grades of intensity selections.
In the present embodiment, control of the control system to the decalcification working time and to decalcification electrolysis strength, can be by decalcification
Course quantization, adjustable decalcification intensity are simultaneously arranged decalcification time, are suitable for various bone tissue decalcifications.
In a preferred embodiment, quick decalcification instrument further includes the power supply for being set to body interior, and the power supply includes electricity
Source adapter and rechargeable battery；The power supply adaptor is used to external reference voltages output be the suitable quick decalcification instrument work
The operating voltage of work includes being for the first power supply adaptor of suitable pump work, by external voltage output by external voltage output
It is suitble to the second source adapter of control system work.
In the present embodiment, after connecting external 220V alternating currents by power cord, pass through the conversion of the first power supply adaptor
It is 20V direct currents for pump work by 220V exchange electricity outputs, by the conversion of second source adapter by 220V alternating currents
Output is electric for the 12V DC to work for control system.To significantly improve the safety in operation of quick decalcification instrument.Power supply mould
Block further includes power switch, the opening and closing for controlling entire quickly decalcification instrument circuit.
Embodiment 2
The present embodiment provides a kind of decalcification methods of quick decalcification instrument, including electrolysis two steps of decalcification and aquation.
Referring to Fig. 3, following steps are specifically included：
S1, decalcifying Fluid is passed through electrolytic cell 1 by decalcification liquid pipe 10 from decalcification liquid bath 4；
S2, electrolysis decalcification is carried out to bone tissue；
S3, the waste liquid after electrolysis decalcification is drained into waste liquid tank 6 by sewer pipe 12 from electrolytic cell 1；
S4, clear water is passed through electrolytic cell 1 by water pipe 11 from Rinsing Area 5；
S5, aquation is carried out to the bone tissue after electrolysis decalcification；
S6, the waste liquid after aquation is drained into waste liquid tank 6 by sewer pipe 12 from electrolytic cell 1；
S7, the bone tissue for obtaining decalcification completion.
In the present embodiment, it will first wait for that the bone tissue of decalcification is put into sample cabin, is then turned on the power switch before decalcification, if
Set decalcification working time and decalcification electrolysis strength.
In the present embodiment, according to bone tissue sample size, more cycles of S1-S3 can be controlled by control system, from
And achieve the purpose that constantly update the decalcifying Fluid in electrolytic cell until electrolysis decalcification is completed.
From above-described embodiment it is found that the quick decalcification instrument of the present invention is using decalcification is electrolysed with decalcifying Fluid, not only greatly greatly
Fast decalcification speed, will not also be caused to damage to bone tissue；Using electrolysis two steps of decalcification and aquation so that of the invention is whole
A decalcification process environmental protection；The quick decalcification instrument of the present invention can be by decalcification course quantization, and simultaneously decalcification is arranged in adjustable decalcification intensity
Time is suitable for various bone tissue decalcifications.
Specific embodiments of the present invention are described in detail above, but it is only used as example, the present invention is not intended to limit
In particular embodiments described above.To those skilled in the art, it is any to the practicality carry out equivalent modifications and replace
In generation, is also all among scope of the invention.Therefore, without departing from the spirit and scope of the invention made by impartial conversion and repair
Change, all should be contained within the scope of the invention.
Claims (9)
1. a kind of quick decalcification instrument, which is characterized in that including main body, reagent trough and control system, wherein：
The sample cabin that the main body includes electrolytic cell, is set in the electrolytic cell；The electrolytic cell by inlet/outlet pipe with it is described
Reagent trough connects；The sample cabin is connected to the electrolytic cell, for placing the bone tissue for waiting for decalcification；
The reagent trough is connect with the electrolytic cell of the main body, for providing the reagent needed for decalcification and holding the useless of decalcification discharge
Liquid；And
Reagent is inputted electrolytic cell by the control system for controlling from the reagent trough, and is carried out to the bone tissue in sample cabin
Decalcification.
2. quick decalcification instrument according to claim 1, which is characterized in that the reagent trough includes decalcification liquid bath, Rinsing Area
And waste liquid tank, the inlet/outlet pipe include for decalcifying Fluid to be passed through the decalcification liquid pipe of the electrolytic cell from the decalcification liquid bath, is used
In clear water is passed through the water pipe of the electrolytic cell, for waste liquid to be passed through the waste liquid from the electrolytic cell from the Rinsing Area
The sewer pipe of slot is separately installed with pump on the decalcification liquid pipe, water pipe and sewer pipe.
3. quick decalcification instrument according to claim 1, which is characterized in that be equipped with and the control system in the electrolytic cell
The liquid level sensor of connection.
4. quick decalcification instrument according to claim 1, which is characterized in that offered on the wall in the sample cabin several logical
Hole, for the connection between the sample cabin and the electrolytic cell.
5. quick decalcification instrument according to claim 1, which is characterized in that the main body further includes shell, on the shell
Equipped with the button, indicator light and liquid crystal display for human-computer interaction being connect with the control system.
6. quick decalcification instrument according to claim 1, which is characterized in that the control system control bone tissue is taken off
Calcium includes the control to the decalcification working time and the control to decalcification electrolysis strength.
7. quick decalcification instrument according to claim 1, which is characterized in that the quick decalcification instrument further include be set to it is described
The power supply of body interior, the power supply include power supply adaptor and rechargeable battery.
8. a kind of method using the quick decalcification instrument electrolysis decalcification as described in claim 1-7, which is characterized in that including following
Step：
S1, decalcifying Fluid is passed through electrolytic cell from reagent trough；
S2, electrolysis decalcification is carried out to bone tissue；
S3, the waste liquid after electrolysis decalcification is drained into reagent trough from electrolytic cell.
9. a kind of method using quickly decalcification instrument aquation as claimed in claim 8, which is characterized in that after S3, including
Following steps：
S4, clear water is passed through electrolytic cell from reagent trough；
S5, aquation is carried out to the bone tissue after electrolysis decalcification；
S6, the waste liquid after aquation is drained into reagent trough from electrolytic cell；
S7, the bone tissue for obtaining decalcification completion.
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| CN201810922736.2A CN108717006A (en) | 2018-08-14 | 2018-08-14 | A kind of quick decalcification instrument and its method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810922736.2A CN108717006A (en) | 2018-08-14 | 2018-08-14 | A kind of quick decalcification instrument and its method |
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