CN104587529A - Method for preparing alveolar bone repair material - Google Patents
Method for preparing alveolar bone repair material Download PDFInfo
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- CN104587529A CN104587529A CN201510006768.4A CN201510006768A CN104587529A CN 104587529 A CN104587529 A CN 104587529A CN 201510006768 A CN201510006768 A CN 201510006768A CN 104587529 A CN104587529 A CN 104587529A
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Abstract
The invention relates to a method for preparing an alveolar bone repair material. The method comprises the following steps: step 1, cutting cancellous bone parts of chine and thigh bone of a mammal into pieces, poaching and drying; step 2, soaking the cancellous bone by using phosphoric acid; step 3, cooking the cancellous bone in a pressure cooker, bleaching by using deionized water, and drying; step 4, modifying the cancellous bone; step 5, calcining the cancellous bone in a high temperature stove; and step 6, crushing and screening the calcined cancellous bone. According to the alveolar bone repair material prepared by utilizing the method, the ratio of hydroxyapatite (HA) to beta-tertiary calcium phosphate (beta-TCP) in the components is between 1:4 and 4:1, the defects that the HA is extremely stable and beta-TCP is extremely quickly degraded are overcome, and the degradation speed of the material can be adjusted by virtue of the ratio between HA and beta-TCP, so that the degradation speed is matched with the growth speed of surrounding bone tissues, and the material has an obvious effect of promoting bone healing. Moreover, the material has a certain hardness and strength and is simple in plasticity and rich in source, and the manufacture problems of artificial synthetic materials in aspects such as porosity, pore traffic and pore size are solved.
Description
Technical field
The invention belongs to biomaterial for medical purpose preparation field, relate to a kind of preparation method of alveolar bone repairing material, fill for alveolar bone, repair.
Background technology
China just steps into aging society, increase rheological properties change and will a large amount of defect and restore demands be caused with the disease occurred, wherein because of Cranial defect problem ubiquity in state's human mouth that absence of tooth causes, agomphosis patient, often with the Alveolar Bone Defect that degree is different, need carry out reconstruction with filling material of bone.Traditional bone grafting timbering material comprises bone-xenograft, homogeneous allogenic bone, synthetic material etc., and above material all has some limitations: homogeneous allogenic bone limited source; There is source and price problem in bone-xenograft, spread disease simultaneously, causes host immune rejection to react equivalent risk; The aspect such as porosity, hole traffic, pore size of synthetic material is different from human body spongy bone, unfavorable to sticking with propagation of cell, the osteoblastic differentiation and maturation of more difficult promotion.
Hydroxyapatite (HA) is the main component of forging bone, is also the main component of inanimate matter in body bone tissue, has good biocompatibility with tissue, but HA degradation in vivo is comparatively slow, and osseous tissue not easily generates in a short time.Bata-tricalcium phosphate (β-TCP) not only has good biocompatibility, and has good biological degradability, its catabolite Ca
2+, PO
4 3-plasma can enter living body fluids, and the formation for new bone provides the beneficial elements such as abundant Ca, P, impels the formation of new bone, but β-TCP degradation speed is in vivo slightly fast, does not mate with new bone growth speed.Therefore, need to study further for alveolar bone repairing material, to meet the needs of alveolar repair.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of alveolar bone repairing material, the alveolar bone repairing material prepared by the method, have certain bone-inducting active to osteogenesis, effectively can promote new life and the reconstruction of the osseous tissue in Alveolar Bone Defect district.
Technical solution of the present invention is as follows:
A preparation method for alveolar bone repairing material, is characterized in that, said method comprising the steps of:
Step one, get mammal spine, the cancellous bone portion of femur be cut into small pieces after decocting in water, drying;
Step 2, soaks dried spongy bone phosphoric acid solution;
Step 3, the spongy bone after being soaked by phosphoric acid solution is positioned over steaming and decocting in pressure cooker, by the rinsed with deionized water several of the spongy bone after steaming and decocting, dry when being 7 to pH value;
Step 4, carries out modification by dried spongy bone;
Step 5, is put into the spongy bone after modification in high temperature furnace and calcines;
Step 6, after the spongy bone fragmentation after calcining, sieves with standard screen.
The preparation method of alveolar bone repairing material as above, preferably, in step one the spongy bone of mammal spine, femur is carried out stripping and slicing, decocting in water, dry method be that the spongy bone of spine, femoral component is cut into 1cm
3fritter, put into pot decocting in water after 3-4 hour, with clear water rinsing several, was placed in drying baker, 120-140 DEG C of dry 6-8 hour.
The preparation method of alveolar bone repairing material as above, preferably, phosphoric acid solution used in step 2, its concentration is 0.5%-5%, and soak time is 0.5-5 hour.
The preparation method of alveolar bone repairing material as above, preferably, in step 3, the temperature of spongy bone steaming and decocting in pressure cooker is 125 DEG C, and the time is 2-4 hour.
The preparation method of alveolar bone repairing material as above, preferably, to the method for spongy bone modification in step 4, spongy bone is immersed in completely the dibastic sodium phosphate solution that concentration is 0.5-5%, or concentration is the disodium phosphate soln of 0.5-5%, soak after 1-3 hour, be positioned in drying baker, 120-140 DEG C of dry 6-8 hour.
The preparation method of alveolar bone repairing material as above, preferably, in step 5, spongy bone is placed in high temperature furnace and calcines, and is after high temperature furnace is warmed up to 300-400 DEG C, insulation 0.5-2 hour; After continuing to be warming up to 500-600 DEG C, insulation 0.5-2 hour; After continuing to be warming up to 700-800 DEG C, insulation 0.5-2 hour; After continuing to be warming up to 900-1000 DEG C, insulation 0.5-2 hour; After continuing to be warming up to 1000-1100 DEG C, insulation 0.5-2 hour.
The preparation method of alveolar bone repairing material as above, preferably, in step 5, the temperature rise speed of high temperature furnace is identical, is 5-20 DEG C/min.
The preparation method of alveolar bone repairing material as above, preferably, in step 5, spongy bone continues to pass into oxygen in high temperature furnace calcination process.
The preparation method of alveolar bone repairing material as above, preferably, in step 6, the spongy bone after calcining being carried out to the method for crushing and screening, is by spongy bone crusher in crushing, with standard screen screening, chooses the spongy bone that granular size scope is 0.01-2.5mm.
Alveolar bone repairing material prepared by the present invention is through the pure inorganic substances of high-temperature calcination, there is not any antigenic substance; Show through X-ray diffraction analysis, it is the double-phase composite containing hydroxyapatite (HA) and bata-tricalcium phosphate (β-TCP); Stereoscan photograph shows, and it remains loose structure and the bone trabecula of nature bone, and, internal surface area similar to human bone structure is large, traffic mutually between hole, is conducive to osteoblastic adhesion and growth, and fiber and blood vessel are grown into.
Advantage of the present invention there is provided a kind of preparation method of alveolar bone repairing material, modification is carried out to forging bone, become the double-phase composite of HA and β-TCP, so both overcome that HA is too stable, β-TCP degrades too fast shortcoming, can, by controlling the degradation speed of proportion adjustment material between the two, its degradation speed and the speed of growth of surrounding bone tissue be matched again; Meanwhile, its porosity, hole traffic, pore size and human body spongy bone are basically identical, are beneficial to the propagation of osteocyte, differentiation.
Figure of description
Fig. 1 is the X-ray diffraction spectrogram of alveolar bone repairing material of the present invention
Fig. 2 is the Electronic Speculum photograph of alveolar bone repairing material of the present invention
Detailed description of the invention:
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Choose the spine spongy bone that 9 healthy, fresh monthly ages hide yak, be cut into 1cm
3fritter, to drop in pot decocting in water 4 hours, rinse for several times with clear water, be placed in drying baker, 120 DEG C of dryings 6 hours.
Dried spongy bone being submerged into concentration is in the phosphoric acid solution of 3% after 0.5 hour, with pressure cooker steaming and decocting 2 hours at 125 DEG C, is 7 for several times, is placed in drying baker by the spongy bone rinsed with deionized water after steaming and decocting to pH value, 120 DEG C of dryings 6 hours.
Spongy bone being immersed in completely concentration is the dibastic sodium phosphate solution soaking of 3% after 1 hour, is positioned in drying baker, 120 DEG C of dry 6-8 hour.
Spongy bone is placed in high temperature furnace and calcines, after high temperature furnace being warmed up to 300 DEG C, be incubated 0.5 hour; After continuing to be warming up to 500 DEG C, be incubated 0.5 hour; After continuing to be warming up to 700 DEG C, be incubated 0.5 hour; After continuing to be warming up to 900 DEG C, be incubated 0.5 hour; After continuing to be warming up to 1000 DEG C, be incubated 0.5 hour.The temperature rise speed of high temperature furnace is identical, is 20 DEG C/min.Continue to pass into oxygen in calcination process.
By the spongy bone crusher in crushing after calcining, with standard screen screening, choose the spongy bone that granular size scope is 0.1-2.0mm.
Claims (9)
1. a preparation method for alveolar bone repairing material, is characterized in that, said method comprising the steps of:
Step one, get mammal spine, the cancellous bone portion of femur be cut into small pieces after decocting in water, drying;
Step 2, soaks dried spongy bone phosphoric acid solution;
Step 3, the spongy bone after being soaked by phosphoric acid solution is positioned over steaming and decocting in pressure cooker, by the rinsed with deionized water several of the spongy bone after steaming and decocting, dry when being 7 to pH value;
Step 4, carries out modification by dried spongy bone;
Step 5, is put into the spongy bone after modification in high temperature furnace and calcines;
Step 6, after the spongy bone fragmentation after calcining, sieves with standard screen.
2. the preparation method of alveolar bone repairing material according to claim 1, is characterized in that: in step one the spongy bone of mammal spine, femur is carried out stripping and slicing, decocting in water, dry method be that the spongy bone of spine, femoral component is cut into 1cm
3fritter, put into pot decocting in water after 3-4 hour, with clear water rinsing several, was placed in drying baker, 120-140 DEG C of dry 6-8 hour.
3. the preparation method of alveolar bone repairing material according to claim 1, is characterized in that: phosphoric acid solution used in step 2, and its concentration is 0.5%-5%, and soak time is 0.5-5 hour.
4. the preparation method of alveolar bone repairing material according to claim 1, is characterized in that: in step 3, the temperature of spongy bone steaming and decocting in pressure cooker is 125 DEG C, and the time is 2-4 hour.
5. the preparation method of alveolar bone repairing material according to claim 1, it is characterized in that: to the method for spongy bone modification in step 4, spongy bone is immersed in completely the dibastic sodium phosphate solution that concentration is 0.5-5%, or concentration is the disodium phosphate soln of 0.5-5%, soak after 1-3 hour, be positioned in drying baker, 120-140 DEG C of dry 6-8 hour.
6. the preparation method of alveolar bone repairing material according to claim 1, is characterized in that: in step 5, spongy bone is placed in high temperature furnace and calcines, and is after high temperature furnace is warmed up to 300-400 DEG C, insulation 0.5-2 hour; After continuing to be warming up to 500-600 DEG C, insulation 0.5-2 hour; After continuing to be warming up to 700-800 DEG C, insulation 0.5-2 hour; After continuing to be warming up to 900-1000 DEG C, insulation 0.5-2 hour; After continuing to be warming up to 1000-1100 DEG C, insulation 0.5-2 hour.
7. the preparation method of alveolar bone repairing material according to claim 1, is characterized in that: in step 5, the temperature rise speed of high temperature furnace is identical, is 5-20 DEG C/min.
8. the preparation method of alveolar bone repairing material according to claim 1, is characterized in that: in step 5, spongy bone continues to pass into oxygen in high temperature furnace calcination process.
9. the preparation method of alveolar bone repairing material according to claim 1, it is characterized in that: the method for in step 6, the spongy bone after calcining being carried out to crushing and screening, be by spongy bone crusher in crushing, with standard screen screening, choose the spongy bone that granular size scope is 0.01-2.5mm.
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| CN201510006768.4A CN104587529A (en) | 2015-01-07 | 2015-01-07 | Method for preparing alveolar bone repair material |
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| CN201510006768.4A CN104587529A (en) | 2015-01-07 | 2015-01-07 | Method for preparing alveolar bone repair material |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106823007A (en) * | 2016-12-30 | 2017-06-13 | 北京爱康宜诚医疗器材有限公司 | Bone renovating material and preparation method thereof |
| CN108261568A (en) * | 2016-12-30 | 2018-07-10 | 深圳兰度生物材料有限公司 | Complex gradient bone renovating material and preparation method thereof |
| CN109662264A (en) * | 2018-07-27 | 2019-04-23 | 王贰瑞 | The processing method and its device of human consumption bone meal material |
| TWI693025B (en) * | 2018-07-27 | 2020-05-11 | 國立屏東科技大學 | Aggregate and fish processing method |
| CN115814160A (en) * | 2022-10-21 | 2023-03-21 | 深圳兰度生物材料有限公司 | Bone repair material and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1422672A (en) * | 2002-11-21 | 2003-06-11 | 上海第二医科大学附属第九人民医院 | Absorbable calcined-bone preparation method |
| US20030135214A1 (en) * | 2002-01-15 | 2003-07-17 | Fetto Joseph F. | System, device, composition and method for treating and preventing avascular or osteonecrosis |
-
2015
- 2015-01-07 CN CN201510006768.4A patent/CN104587529A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030135214A1 (en) * | 2002-01-15 | 2003-07-17 | Fetto Joseph F. | System, device, composition and method for treating and preventing avascular or osteonecrosis |
| CN1422672A (en) * | 2002-11-21 | 2003-06-11 | 上海第二医科大学附属第九人民医院 | Absorbable calcined-bone preparation method |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106823007A (en) * | 2016-12-30 | 2017-06-13 | 北京爱康宜诚医疗器材有限公司 | Bone renovating material and preparation method thereof |
| CN108261568A (en) * | 2016-12-30 | 2018-07-10 | 深圳兰度生物材料有限公司 | Complex gradient bone renovating material and preparation method thereof |
| CN109662264A (en) * | 2018-07-27 | 2019-04-23 | 王贰瑞 | The processing method and its device of human consumption bone meal material |
| TWI693025B (en) * | 2018-07-27 | 2020-05-11 | 國立屏東科技大學 | Aggregate and fish processing method |
| CN115814160A (en) * | 2022-10-21 | 2023-03-21 | 深圳兰度生物材料有限公司 | Bone repair material and preparation method thereof |
| CN115814160B (en) * | 2022-10-21 | 2024-02-06 | 深圳兰度生物材料有限公司 | Bone repair material and preparation method thereof |
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Address after: 102200 Beijing Changping District Science Park Road No. 10 Bai Fu industrial building two layer Applicant after: Beijing AKEC Medical Co., Ltd. Address before: 102200 Beijing Changping District Science Park Road No. 10 Bai Fu industrial building two layer Applicant before: Beijing AKEC Medical Co.,Ltd. |
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Application publication date: 20150506 |
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