CN102668909A - Method for rejecting abnormal plants through resistance identification of insect-resistant and herbicide-resistant transgenic double-gene cottons - Google Patents
Method for rejecting abnormal plants through resistance identification of insect-resistant and herbicide-resistant transgenic double-gene cottons Download PDFInfo
- Publication number
- CN102668909A CN102668909A CN2012101828710A CN201210182871A CN102668909A CN 102668909 A CN102668909 A CN 102668909A CN 2012101828710 A CN2012101828710 A CN 2012101828710A CN 201210182871 A CN201210182871 A CN 201210182871A CN 102668909 A CN102668909 A CN 102668909A
- Authority
- CN
- China
- Prior art keywords
- resistance
- resistant
- cotton
- rejecting
- leaves
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a method for rejecting abnormal plants through resistance identification of insect-resistant and herbicide-resistant transgenic double-gene cottons. The method comprises the following steps: spraying a rejecting liquor, which is formed by 41% glyphosate isopropamide of 3.3 ml/L and 500 thousand unit kanamycin of 4000 mg/L, on insect-resistant and herbicide-resistant transgenic double-gene cotton seedlings at the two-leaf and one-bud stage by using a sprayer; and recording spray burn grades of the cotton seedlings one to one when 10 days pass away after spraying the rejecting liquor, wherein a high resistance grade is as follows: the cotton seedlings, 95-100% seed leaves and main leaves of which are not discolored and grow in a normal state, belong to a variety without resistance degradation; a resistance grade is as follows: the cotton seedlings, 90% seed leaves and main leaves of which turn yellow, belong to a variety with degraded resistance; a micro resistance grade is as follows: the cotton seedlings, 80% seed leaves and main leaves of which turn yellow and are locally withered due to water loss, belong to a variety with seriously degraded resistance; and a resistance-free grade is as follows: the cotton seedlings, 70% seed leaves of which are withered and main leaves and interior leaves of which are blackened and rotten, belong to a variety without resistance; and the method for rejecting the abnormal plants through resistance identification of the insect-resistant and herbicide-resistant transgenic double-gene cottons, provided by the invention, has the advantages of accuracy for identification, high speed and simplicity for preparing the rejecting liquor.
Description
Technical field
The present invention relates to the cotton resistance of a kind of commentaries on classics divalent insect-resistant anti-herbicide gene (Cry1Ac+EPSPS) and identify the method for assorted strain.
Background technology
Cotton variety is in planting process, because artificial and natural factors such as (bird, insects) can cause kind to mix, degenerate, thereby influences output, and traditional improving one's methods is the seed with cultivation method purification and rejuvenation kind.Twentieth century the mid-90; Promptly facing the varietal resistance degeneration after soil of transgenic Bt cotton is come out descends and seed hydridization problem; The scientific research personnel of The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute uses kanamycin 4000mg/l dilution and when the nursery stage cotton grows 1-2 sheet true leaf, sprays for this reason; Then according to cotton leaf whether flavescence reject the cotton assorted strain of non-transgenic in the soil of transgenic Bt cotton (the blade yellow reaction be the cotton assorted strain of non-transgenic; It is soil of transgenic Bt cotton that leaf color keeps green), simultaneously again can be from the resistance rank (kind resistance to insects degree of degeneration) of this soil of transgenic Bt cotton resistance to insects of cotton leaf yellow percentage evaluation.Changeing divalent insect-resistant anti-herbicide gene (Cry1Ac+EPSPS) cotton is commentaries on classics divalent insect-resistant resistance glyphosate weed killer herbicide gene cotton new on the cotton of coming out in 2010, and still present this new divalent insect-resistant resistance glyphosate weed killer herbicide gene cotton also lacks the resistance of supporting special use and identifies mix strain rejecting agent and method.
Summary of the invention
The object of the invention just provides the cotton resistance of a kind of evaluation accurate, fireballing commentaries on classics divalent insect-resistant anti-herbicide gene (Cry1Ac+EPSPS) and identifies assorted strain elimination method.
The cotton resistance of commentaries on classics divalent insect-resistant anti-herbicide gene of the present invention is identified the method for assorted strain; It is rejecting liquor with 41% glyphosate isopropyl amine salt 3.3ml/L+, the 500000 kanamycin 4000mg/L of unit; Changeing on divalent insect-resistant Antiglyphosate gene (Cry1Ac+EPSPS) the two leaves cotton seedling of one heart stage with atomizers spray; Spray behind the medicine 10 days by strain record poisoning rank, pest-resistant antiweed property grade scale:
High anti-level: the cotton young plant leaf of 95%-100%, true leaf nondiscolouring, growth is normal, and belonging to varietal resistance does not have degeneration;
Anti-level: the cotton young plant leaf more than 90%, true leaf flavescence belong to varietal resistance and degenerate;
Little anti-level: the cotton young plant leaf more than 80%, true leaf flavescence, local dehydration is withered soft, belongs to the varietal resistance serious degradation;
Not anti-level: the cotton young plant leaf more than 70% is withered, and true leaf and lobus cardiacus blackening are withered mashed, belongs to kind and has lost resistance;
Sprayed behind the medicine cotton seedling qualitative 18 days:
The cotton seedling of high pest-resistant resistance glyphosate: 18 days cotyledons keep green constant with true leaf behind the medicine;
The cotton seedling of pest-resistant resistance glyphosate: 18 days cotyledons, true leaf greens yellowing of going down behind the medicine;
The cotton seedling of the glyphosate tolerant of anti-the worm: cotyledon, true leaf yellowing in 18 days become behind the medicine has young leaves to grow after withered;
The cotton seedling of the assorted strain of not pest-resistant resistance glyphosate: 18 days cotyledon yellow chlorosis are withered behind the medicine, true leaf and lobus cardiacus blackening, and complete stool is withered.
The method of the assorted strain of the cotton resistance evaluation of commentaries on classics divalent insect-resistant anti-herbicide gene of the present invention, evaluation is accurate, speed is fast, and preparation rejecting liquor is simple.
Embodiment
A kind of method of changeing the assorted strain of the cotton resistance evaluation of divalent insect-resistant anti-herbicide gene; Be rejecting liquor, changeing on divalent insect-resistant Antiglyphosate gene (Cry1Ac+EPSPS) the two leaves cotton seedling of one heart stage with atomizers spray with 41% glyphosate isopropyl amine salt 3.3ml/L+, the 500000 kanamycin 4000mg/L of unit.
Rejecting liquor is to be processed by following percentage by weight raw material: glyphosate isopropyl amine salt 0.1353%, kanamycin 4%, water surplus; Its preparation method be with two kinds of medicament mixed dissolutions in water; Resistance is identified in the assorted strain rejecting liquor; Glyphosate isopropyl amine salt content 0.1353g/L, the medicine content 4000mg/L of 500,000 unit kanamycin.
Spray behind the medicine 10 days by strain record poisoning rank, pest-resistant antiweed property grade scale:
High anti-level: the cotton young plant leaf of 95%-100%, true leaf nondiscolouring, growth is normal, and belonging to varietal resistance does not have degeneration;
Anti-level: the cotton young plant leaf more than 90%, true leaf flavescence belong to varietal resistance and degenerate;
Little anti-level: the cotton young plant leaf more than 80%, true leaf flavescence, local dehydration is withered soft, belongs to the varietal resistance serious degradation;
Not anti-level: the cotton young plant leaf more than 70% is withered, and true leaf and lobus cardiacus blackening are withered mashed, belongs to kind and has lost resistance.
Claims (2)
1. one kind is changeed the method that the cotton resistance of divalent insect-resistant anti-herbicide gene is identified assorted strain; It is characterized in that: with the rejecting liquor of 41% glyphosate isopropyl amine salt 3.3ml/L+, the 500000 kanamycin 4000mg/L of unit; Changeing on the divalent insect-resistant Antiglyphosate gene two leaves cotton seedling of one heart stage with atomizers spray; Spray behind the medicine 10 days by strain record poisoning rank, pest-resistant antiweed property grade scale:
High anti-level: the cotton young plant leaf of 95%-100%, true leaf nondiscolouring, growth is normal, and belonging to varietal resistance does not have degeneration;
Anti-level: the cotton young plant leaf more than 90%, true leaf flavescence belong to varietal resistance and degenerate;
Little anti-level: the cotton young plant leaf more than 80%, true leaf flavescence, local dehydration is withered soft, belongs to the varietal resistance serious degradation;
Not anti-level: the cotton young plant leaf more than 70% is withered, and true leaf and lobus cardiacus blackening are withered mashed, belongs to kind and has lost resistance.
2. the cotton resistance of commentaries on classics divalent insect-resistant anti-herbicide gene according to claim 1 is identified the method for assorted strain; It is characterized in that: the rejecting liquor of said 41% glyphosate isopropyl amine salt 3.3ml/L+, the 500000 kanamycin 4000mg/L of unit is to be processed by following percentage by weight raw material: glyphosate isopropyl amine salt 0.1353%, kanamycin 4%, water surplus, its preparation method be with two kinds of medicament mixed dissolutions in water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101828710A CN102668909A (en) | 2012-06-06 | 2012-06-06 | Method for rejecting abnormal plants through resistance identification of insect-resistant and herbicide-resistant transgenic double-gene cottons |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101828710A CN102668909A (en) | 2012-06-06 | 2012-06-06 | Method for rejecting abnormal plants through resistance identification of insect-resistant and herbicide-resistant transgenic double-gene cottons |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102668909A true CN102668909A (en) | 2012-09-19 |
Family
ID=46802004
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101828710A Pending CN102668909A (en) | 2012-06-06 | 2012-06-06 | Method for rejecting abnormal plants through resistance identification of insect-resistant and herbicide-resistant transgenic double-gene cottons |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102668909A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1360826A (en) * | 2000-12-27 | 2002-07-31 | 朱安灿 | Field distinguishing agent for transgenic cotton and its application method |
| CN1412323A (en) * | 2002-11-25 | 2003-04-23 | 中山大学 | Method for screening transgenic plant and its used bivalent plant expression vector |
-
2012
- 2012-06-06 CN CN2012101828710A patent/CN102668909A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1360826A (en) * | 2000-12-27 | 2002-07-31 | 朱安灿 | Field distinguishing agent for transgenic cotton and its application method |
| CN1412323A (en) * | 2002-11-25 | 2003-04-23 | 中山大学 | Method for screening transgenic plant and its used bivalent plant expression vector |
Non-Patent Citations (3)
| Title |
|---|
| 叶鹏盛等: "利用卡那霉素间接鉴定转基因抗虫棉的研究", 《四川农业大学学报》 * |
| 王景雪等: "油菜转抗草甘膦、抗虫基因获得双抗植株", 《遗传学报》 * |
| 谢龙旭: "抗除草剂草甘膦抗虫双抗转基因棉花的创制", 《中山大学博士学位论文》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Blum | Sorghum physiology | |
| CN105010129B (en) | Precocious upland cotton new germ plasm selection | |
| CN101960987A (en) | Seed production method for hybrid rice | |
| CN106900346A (en) | Food sweet potato gently simplifies potato root rattan method for culturing seedlings | |
| CN102301948A (en) | Method for breeding high-yield and disease-resistant glutinous rice | |
| CN104285779A (en) | Method for hybridizing and breeding fast-growing cold-resistant poplar varieties | |
| Sanaev et al. | Yield and marketability of potato varieties with different numbers of irrigation schemes and fertilizer standards in case of Uzbekistan | |
| CN105960980B (en) | Ramie cultivation and harvesting method with fiber feeding and harvesting functions | |
| AU2015202003A1 (en) | A method of in vitro culture of wheat spikes through hybridization between wheat and maize to induce haploid embryos | |
| CN102771382B (en) | Cultivating method for japonica rice variety strong in lodging resistance and suitable for mechanical transplanting of rice | |
| CN106069424A (en) | The implantation methods of perennial root Oryza sativa L. | |
| CN104255431A (en) | Breeding method of nitrogen fixing sugarcane | |
| CN103329794A (en) | Method of intergeneric hybridization between longans and litchis | |
| CN109997684A (en) | A kind of breeding of rice varieties and its cultural method | |
| CN113016518A (en) | Resistance identification method for celery leaf spot | |
| CN107646373A (en) | A kind of grafting cultivation method of tomato and water planting potato | |
| CN112205228A (en) | Cultivation method for artificially promoting natural renewal of litsea cubeba | |
| CN104956868A (en) | Interplanting method for peanuts and daylily on red soil gentle slope dry land | |
| CN102771386B (en) | Breeding method of rice | |
| CN102668909A (en) | Method for rejecting abnormal plants through resistance identification of insect-resistant and herbicide-resistant transgenic double-gene cottons | |
| Nabizadeh et al. | Effect of pruning lateral branches on four varieties of medicinal castor bean plant (Ricinuscommunis L.) yield, growth and development | |
| CN114451299A (en) | Wild flower kiwi fruit male parent creation with high pollen forming capacity and application thereof | |
| Chen et al. | Tea plant (Camellia sinensis) breeding in Korea | |
| CN109511407B (en) | Method for rapidly identifying drought tolerance of radish | |
| CN106718828A (en) | A kind of breeding method of drought-enduring type rice varieties |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120919 |