CN102659792A - Method for extracting chlorophyll from clovers - Google Patents
Method for extracting chlorophyll from clovers Download PDFInfo
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- CN102659792A CN102659792A CN2012101365051A CN201210136505A CN102659792A CN 102659792 A CN102659792 A CN 102659792A CN 2012101365051 A CN2012101365051 A CN 2012101365051A CN 201210136505 A CN201210136505 A CN 201210136505A CN 102659792 A CN102659792 A CN 102659792A
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- trifolium
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Abstract
The invention discloses a method for extracting chlorophyll from clovers, comprising the following steps of: (1) preparing raw materials; (2) granulating; (3) leaching; (4) filtering; (5) concentrating and recycling; and (6) removing solvent residues. The chlorophyll prepared by the method disclosed by the invention is a natural plant pigment so that the chlorophyll has strong safety and is harmless to human bodies; and the chlorophyll not only can be used as a coloring agent, but also has higher nutritional values and pharmacological values, so that the chlorophyll can be widely applied to foods, cosmetics, medicines and health-care products.
Description
Technical field
The present invention relates to a kind of chlorophyllous method of from biology, extracting, especially a kind ofly from trifolium, extract chlorophyllous method.
Background technology
Chlorophyll is very important additive in the food as a kind of natural food colour, also can be used as the tinting material on makeup and the pharmaceutical prod and certain medical use is arranged, and medical findings shows that chlorophyll has significant liver protecting function.Mostly chlorophyll on the market is synthetic or animal pigment, and therefore, the research of extracting the best approach for natural chlorophyll has great value and significance.It is rare from trifolium, to extract chlorophyllous report, because the content in the trifolium is lower, but trifolium is cheap and very easy plantation has resources advantage widely, helps suitability for industrialized production, and therefore vast potential for future development is arranged.Therefore, studying a kind of chlorophyllous method of from trifolium, extracting, is the present technical issues that need to address.
Summary of the invention
Technical problem to be solved by this invention provides a kind of chlorophyllous method of from trifolium, extracting.
The technical scheme that the present invention solves its technical problem is:
A kind ofly from trifolium, extract chlorophyllous method, may further comprise the steps:
1. raw material is prepared, and the trefoil leaf that is about to gather or purchase separates with stem, stays leaf to remove stem;
2. granulate, be about to the trefoil leaf that 1. step obtain and squeeze into the trifolium particle;
3. leaching is about to the trifolium particle that 2. a certain amount of step squeeze and drops in the retort, adds a certain amount of extraction agent, under 55 ℃ of temperature, leaches 4 hours;
4. filter, be about to step leaching mixture 3. and get leach liquor after with the strainer multiple times of filtration;
5. concentrate to reclaim, be about to the leach liquor that step obtains in 4. and in the vaporizer that possesses certain vacuum degree and temperature, concentrate, the gained resistates is chlorophyll medicinal extract work in-process behind the recovery solvent;
6. desolventizing is residual; Be about to chlorophyll medicinal extract work in-process short-path distillation in possessing the short-path distillation tower of certain vacuum degree and temperature that 5. step obtains; Remove the dissolvent residual in the chlorophyll medicinal extract work in-process, dissolvent residual reaches certain standard and is chlorophyll medicinal extract finished product.
Preferably, described extraction agent is an acetone.
Preferably, the mass ratio 1:12 of trifolium particle and extraction agent.
Preferably, described cascade filtration is that Pyatyi is filtered, and the mesh number of grade one filter is 60 orders; The mesh number of secondary filter is 80 orders; The mesh number of tertiary filter is 120 orders, and the mesh number of level Four strainer is 180 orders, and the mesh number of Pyatyi strainer is 200 orders.
Preferably, the vacuum tightness of vaporizer is-the relative vacuum degree of 0.08Mpa, temperature is 40 ℃.
Preferably, the vacuum tightness of short-path distillation tower is 0.4～40Pa, and temperature is 35 ℃.
Preferably, the dissolvent residual standard of chlorophyll medicinal extract finished product is less than 25ppm.
The present invention extracts chlorophyllous method and extracts chlorophyllous method with other and compare from trifolium, have the following advantages:
1. cost is low: because the trifolium regenerative power is more intense, plant more extensively, resource rich, thereby less expensive reduces production costs.
2. raw material availability is high: the raw material squeezing is particle, and what the space within the particle helped extraction liquid and raw material fully contacts the raising yield.
3. expect that the slag income is high: the material slag crude protein content that trifolium is extracted behind the chlorophyll is high, can be used as animal-feed, improves income.
The chlorophyll that the present invention extracts from trifolium is compared with other chlorophyll, has the following advantages:
1. physical method extracts: mostly the chlorophyll on the market is chemosynthesis or animality pigment, and pure physical method extraction is taked in the chlorophyllous extraction among the present invention, is the fully natural green plant pigments, and high safety is harmless to humans and animals.
2. trophic function is strong: the natural pigment that from plant, extracts has trophic function and the pharmacological function of corresponding plants, except that as the tinting material, can also be used as in food, medicine and the healthcare products.
3. coloring effect is different: this chlorophyll medicinal extract is natural pigment, becomes chromatic effect to show as soft, the nature of color and luster.
Embodiment
Combine embodiment to further specify the present invention at present.
Embodiment
The trifolium particle 1000 that squeezing is good restrains, and drops into retort, adds acetone 12000 grams, extracts 4 hours down at 55 ℃.Extraction liquid is sent into strainer, after filtering through Pyatyi, in vaporizer, reclaims solvent, and control relative vacuum degree is-0.08MPa that temperature is 40 ℃.After being evaporated to 1/4～1/10 volume, send into the short-path distillation tower to medicinal extract, control perfect vacuum degree is 0.4～40Pa, and temperature is 35 ℃, and when dissolvent residual was lower than 25ppm, the gained paste was finished product chlorophyll medicinal extract.
Claims (7)
1. one kind is extracted chlorophyllous method from trifolium, it is characterized in that: may further comprise the steps:
1. raw material is prepared, and the trefoil leaf that is about to gather or purchase separates with stem, stays leaf to remove stem;
2. granulate, be about to the trefoil leaf that 1. step obtain and squeeze into the trifolium particle;
3. leaching is about to the trifolium particle that 2. a certain amount of step squeeze and drops in the retort, adds a certain amount of extraction agent, under 55 ℃ of temperature, leaches 4 hours;
4. filter, be about to step leaching mixture 3. and get leach liquor after with the strainer multiple times of filtration;
5. concentrate to reclaim, be about to the leach liquor that step obtains in 4. and in the vaporizer that possesses certain vacuum degree and temperature, concentrate, the gained resistates is chlorophyll medicinal extract work in-process behind the recovery solvent;
6. desolventizing is residual; Be about to chlorophyll medicinal extract work in-process short-path distillation in possessing the short-path distillation tower of certain vacuum degree and temperature that 5. step obtains; Remove the dissolvent residual in the chlorophyll medicinal extract work in-process, dissolvent residual reaches certain standard and is chlorophyll medicinal extract finished product.
2. a kind of chlorophyllous method of from trifolium, extracting according to claim 1, it is characterized in that: described extraction agent is an acetone.
3. a kind of chlorophyllous method of from trifolium, extracting according to claim 1 is characterized in that: the mass ratio 1:12 of trifolium particle and extraction agent.
4. a kind of chlorophyllous method of from trifolium, extracting according to claim 1; It is characterized in that: described cascade filtration is that Pyatyi is filtered; The mesh number of grade one filter is 60 orders, and the mesh number of secondary filter is 80 orders, and the mesh number of tertiary filter is 120 orders; The mesh number of level Four strainer is 180 orders, and the mesh number of Pyatyi strainer is 200 orders.
5. a kind of chlorophyllous method of from trifolium, extracting according to claim 1 is characterized in that: the vacuum tightness of vaporizer is the relative vacuum degree of-0.08Mpa, and temperature is 40 ℃.
6. a kind of chlorophyllous method of from trifolium, extracting according to claim 1, it is characterized in that: the vacuum tightness of short-path distillation tower is 40Pa, temperature is 35 ℃.
7. a kind of chlorophyllous method of from trifolium, extracting according to claim 1, it is characterized in that: the dissolvent residual standard of chlorophyll medicinal extract finished product is less than 25ppm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101365051A CN102659792A (en) | 2012-05-05 | 2012-05-05 | Method for extracting chlorophyll from clovers |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012101365051A CN102659792A (en) | 2012-05-05 | 2012-05-05 | Method for extracting chlorophyll from clovers |
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| CN102659792A true CN102659792A (en) | 2012-09-12 |
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| CN2012101365051A Pending CN102659792A (en) | 2012-05-05 | 2012-05-05 | Method for extracting chlorophyll from clovers |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103641838A (en) * | 2013-11-27 | 2014-03-19 | 威海市桢昊生物技术有限公司 | Method for extraction of chlorophyll from Lactuca sativa |
| CN104429847A (en) * | 2014-10-24 | 2015-03-25 | 桂林丰润莱生物科技有限公司 | Method for planting Dendrobiumofficinale through residues of plants with effective constituents extracted |
| CN104678066A (en) * | 2015-03-17 | 2015-06-03 | 内蒙古民族大学 | Method for recycling chlorophyll solution |
| CN106518884A (en) * | 2016-10-21 | 2017-03-22 | 乌鲁木齐上善元生物科技有限公司 | Method for extraction of chlorophyll from safflower seedlings |
| CN107033626A (en) * | 2017-04-28 | 2017-08-11 | 常德华馥生物技术有限公司 | A kind of method that subcritical abstraction is combined separate tobacco natural pigment with molecular distillation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1696130A (en) * | 2005-06-03 | 2005-11-16 | 四川通世达实业集团生物科技制品有限公司 | Method for continuous extracting chlorophyll from silkworm dropping |
| CN1827620A (en) * | 2006-04-05 | 2006-09-06 | 辽宁省农业科学院食品与加工研究所 | Process for preparing natural chlorophyll and products thereof with spearmint as raw material |
| RU2294209C1 (en) * | 2006-02-01 | 2007-02-27 | Общество с ограниченной ответственностью "БАЙОЮНИКТЕХ" | Immunomodulating preparation |
-
2012
- 2012-05-05 CN CN2012101365051A patent/CN102659792A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1696130A (en) * | 2005-06-03 | 2005-11-16 | 四川通世达实业集团生物科技制品有限公司 | Method for continuous extracting chlorophyll from silkworm dropping |
| RU2294209C1 (en) * | 2006-02-01 | 2007-02-27 | Общество с ограниченной ответственностью "БАЙОЮНИКТЕХ" | Immunomodulating preparation |
| CN1827620A (en) * | 2006-04-05 | 2006-09-06 | 辽宁省农业科学院食品与加工研究所 | Process for preparing natural chlorophyll and products thereof with spearmint as raw material |
Non-Patent Citations (1)
| Title |
|---|
| 张志强，等: "三叶草中叶绿素提取方法的研究", 《中国食品添加剂》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103641838A (en) * | 2013-11-27 | 2014-03-19 | 威海市桢昊生物技术有限公司 | Method for extraction of chlorophyll from Lactuca sativa |
| CN104429847A (en) * | 2014-10-24 | 2015-03-25 | 桂林丰润莱生物科技有限公司 | Method for planting Dendrobiumofficinale through residues of plants with effective constituents extracted |
| CN104678066A (en) * | 2015-03-17 | 2015-06-03 | 内蒙古民族大学 | Method for recycling chlorophyll solution |
| CN106518884A (en) * | 2016-10-21 | 2017-03-22 | 乌鲁木齐上善元生物科技有限公司 | Method for extraction of chlorophyll from safflower seedlings |
| CN107033626A (en) * | 2017-04-28 | 2017-08-11 | 常德华馥生物技术有限公司 | A kind of method that subcritical abstraction is combined separate tobacco natural pigment with molecular distillation |
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Application publication date: 20120912 |