CN104497057A - Process for extracting tea polyphenols from Yunan dayezhong tea leaves - Google Patents
Process for extracting tea polyphenols from Yunan dayezhong tea leaves Download PDFInfo
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- CN104497057A CN104497057A CN201410801452.XA CN201410801452A CN104497057A CN 104497057 A CN104497057 A CN 104497057A CN 201410801452 A CN201410801452 A CN 201410801452A CN 104497057 A CN104497057 A CN 104497057A
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Abstract
The invention discloses a process for extracting tea polyphenols from Yunan dayezhong tea leaves, which is implemented by taking low-value waste broken tea produced in the process of steaming fresh leaves on tea trees on annual pruning branches of Yunan dayezhong into raw green tea or in the process of producing famous high-quality tea as a raw material through the steps of raw material preparation, extraction, coarse filtration, reverse osmosis concentration, extraction, recovery and concentration, spray drying, sterilization and packaging, so that high-purity tea polyphenols are obtained. According to the method, ethyl acetate is adopted for being prepared into tea polyphenols by extracting, the whole process is implemented under atmospheric pressure conditions, no toxic solvent is used, and no heavy metal residue is produced, and therefore, according to the process, the safety is high, the control is easy, the production time is short, the production and operation are stable, and the investment and running costs of equipment are relatively low. The tea polyphenol content of products prepared according to the method can be more than 90%, and the yield of tea polyphenols is 9-11%.
Description
Technical field
The invention belongs to processing field of tea leaves, specifically a kind of technique extracting tea-polyphenol from big-leaf species in yunnan tealeaves.
Background technology
The traditional method preparing tea-polyphenol at present from tealeaves is mainly divided into following a few class.Ion catch electron microscopy: be utilize metal to precipitate tea-polyphenol, and make it be separated with trimethyl-xanthine, as mantoquita, lead salt or aluminum chloride.The method use the heavy metal poisonous to human body and make precipitation agent, its product can not accept for food and medicine is industrial.
Supercritical CO
2extract: be by gaseous state CO
2become liquid state through ultra-high voltage thus extract, its advantage makes consumption of organic solvent reduce, pollutes less, residual solvent can be completely removed, extraction yield is high, but its security is not high, equipment investment is large, operability is poor.Column chromatography for separation preparation method: have gel column, adsorption column and ion exchange column.The key of technique is selection and the drip washing of column packed material.Research shows, adopt post to be separated preparation method catechin yield between 4% ~ 8%, purity can reach 98.1%, can height caffein-free as being separated with gel column, and its residual quantity is only 0.1%.But column packed material is as very expensive in adsorptive type resin, lipophilic gel etc., and with multiple, a large amount of organic solvent during drip washing, separation cycle is longer.
Supercritical extraction only rests on laboratory stage at present.This method is using liquid carbon dioxide as extraction agent, and product without any dissolvent residual, but because cost is high, also cannot be applied in the present circumstance.
Solvent-extraction process: be leach from tealeaves with polar solvent, then leaching liquid carried out liquid-liquid extracting and separating, finally concentrate and obtain product.Current suitability for industrialized production catechin mainly adopts this method to produce, and product yield is 5% ~ 6%, and the purity of product is about 60% ~ 80%, EGCG40% ~ 50%.Organic solvent used is as acetone, ether, methyl alcohol, hexane and trichloromethane etc.
Solvent extration is the main production of current tea-polyphenol, and the polyphenol content that this method is produced is high, and yield is high, and cost is low.The method uses multiple organic solvent, and production cost is high, and the organic solvent of some toxic substance makes product and operates safe not to the utmost, and easily causes environmental pollution.There is serious edible safety hidden danger in product, thus hinders the widespread use of tea leaf extract tea-polyphenol in fields such as edible, pharmacy.In addition, because solvent is recycled repeatedly not only cause a large amount of energy consumptions, and cause the uncontrollability in solvent use procedure, in production waste, have dissolvent residual, bring difficulty to the sewage treatment works in manufacturer's later stage, make pollutant discharge of enterprise be difficult to up to standard or processing cost is too high.
Summary of the invention
The object of the invention is to for above-mentioned the deficiencies in the prior art, a kind of technique extracting tea-polyphenol from big-leaf species in yunnan tealeaves is provided, the gross tea that starting material are the annual pruning branch processing of big-leaf species in yunnan and the low value produced when producing Famous High-quality Tea discard fannings, pass through technology popularization, one is scrap feed material, turn waste into wealth, increase farmers' income, two is that effective constituent in tealeaves is applied to chemical industry, medicine, health products trade, extend Tea Industry chain, promote tea product value added, promote Development of Tea Industry.
The present invention is achieved by following technical solution:
From big-leaf species in yunnan tealeaves, extract a technique for tea-polyphenol, comprise the steps:
Step 1 raw materials: the fresh leaves of tea plant baking on annual for big-leaf species in yunnan pruning branch is become green gross tea, yield is 25% in mass ratio; The raw product of the green gross tea system of the baking as raw material fresh leaves of tea plant preliminary working, not of uniform size, shape is coarse.
Step 2 is extracted: adopt pure water or deionized water to extract green gross tea, condition is: with 80 DEG C of water extraction 2 times, staticly extracts, and first time adds 7 times of water gagings, extracts 40min, and second time adds 6 times of water gagings, extraction 30min; The extracting solution of merging is cooled to 20 DEG C, obtains cooling fluid;
Step 3 coarse filtration: cooling fluid adopted tripod pendulum type batch centrifugal to carry out continuously centrifuged separation, removing impurity, obtains clear liquor;
Step 4 concentrates: adopt reverse osmosis concentration technology to concentrate clear liquor;
Step 5 extracts: adopt vinyl acetic monomer to extract concentrated solution, extract 2 times, add the vinyl acetic monomer of concentrated solution 1.5 times amount for the first time, dynamic extraction 40min; Add the vinyl acetic monomer of concentrated solution 1.5 times amount again, leave standstill layering in 30 minutes, obtain extraction liquid;
Step 6 reclaims concentrated: adopt reverse osmosis concentration technology to concentrate extraction liquid;
Step 7 spraying dry: the inlet temperature 180 DEG C controlling spray-drier, air outlet temperature 80 DEG C, adopts closed system;
Step 8 sterilising packaging: adopt ozone sterilization technology, sterilising treatment is carried out to product;
Obtain the product of polyphenol content more than 98% by technique of the present invention, tea-polyphenol yield is at 9.6%-10.6%.
The low value that described raw material can also adopt the production tea time to produce discards fannings.
The ethyl acetate extraction that the present invention uses is as solvent, avoid the use of the organic solvents such as acetone, ether, methyl alcohol, hexane and trichloromethane, and carry out under atmospheric pressure state, use low temperature, production time short, its advantage is, without the use of noxious solvent, heavy metal free remains, and security is high, and process is easy to control, production operation is stablized, and input and the running cost of equipment are lower.Utilize this legal system to obtain product that polyphenol content can reach more than 90%, tea-polyphenol yield is between 9-11%.
Embodiment
embodiment:
Prepared by step 1 gross tea: the fresh leaves of tea plant baking on annual for 120 kg big-leaf species in yunnan pruning branch is become green gross tea, and yield is 25%, can obtain 30 kilograms of green gross tea of baking.
Step 2 is extracted: adopt pure water or deionized water to extract green gross tea, condition is: with 80 DEG C of water extraction 2 times, first time adds 7 times of water gagings, extraction 40min; Second time adds 6 times of water gagings, extracts 30min, is extracted by static state, and united extraction liquid is cooled to 20 DEG C and obtains cooling fluid.
Step 3 coarse filtration: cooling fluid adopted tripod pendulum type batch centrifugal to carry out continuously centrifuged separation, removing impurity, obtains clear liquor.
Step 4 concentrates: adopt reverse osmosis concentration technology to concentrate clear liquor.
Step 5 extracts: adopt vinyl acetic monomer to extract concentrated solution, extract 2 times, add the vinyl acetic monomer of concentrated solution 1.5 times amount for the first time, dynamic extraction 40min; Add the vinyl acetic monomer of concentrated solution 1.5 times amount again, leave standstill layering in 30 minutes, be extracted liquid.
Step 6 reclaims concentrated: adopt reverse osmosis concentration technology to concentrate extraction liquid.
Step 7 spraying dry: the inlet temperature 180 DEG C controlling spray-drier, air outlet temperature 80 DEG C, adopts closed system.
Step 8 sterilising packaging: adopt ozone sterilization technology, sterilising treatment is carried out to product.
By technique scheme, 30 kilograms of green gross tea obtain the tea-polyphenol 2.9-3.2 kilogram of content more than 98%, and yield is between 9.6%-10.6%.
Claims (3)
1. from big-leaf species in yunnan tealeaves, extract the technique of tea-polyphenol, it is characterized in that, step is as follows:
Step 1) raw materials: the fresh leaves of tea plant baking on annual for big-leaf species in yunnan pruning branch is become green gross tea, yield is 25% in mass ratio;
Step 2) extract: adopt pure water or deionized water to extract green gross tea, 80 DEG C of water extraction 2 times, staticly to extract, first time adds 7 times of water gagings, extracts 40min, and second time adds 6 times of water gagings, extracts 30min; United extraction liquid is cooled to 20 DEG C;
Step 3) coarse filtration: cooling fluid adopted tripod pendulum type batch centrifugal to carry out continuously centrifuged separation, removing impurity, obtains clear liquor;
Step 4) concentrates: adopt reverse osmosis concentration technology to carry out concentratedly obtaining concentrated solution to clear liquor;
Step 5) extracts: adopt vinyl acetic monomer to extract concentrated solution, extract 2 times, add the vinyl acetic monomer of concentrated solution 1.5 times amount for the first time, dynamic extraction 40min; Add the vinyl acetic monomer of concentrated solution 1.5 times amount again, leave standstill layering in 30 minutes, be extracted liquid;
Step 6) reclaims concentrated: adopt reverse osmosis concentration technology to concentrate extraction liquid;
Step 7) spraying dry: the inlet temperature 180 DEG C controlling spray-drier, air outlet temperature 80 DEG C, adopts closed system;
Step 8) sterilising packaging: adopt ozone sterilization technology, sterilising treatment is carried out to product.
2. a kind of technique extracting tea-polyphenol from big-leaf species in yunnan tealeaves according to claim 1, is characterized in that, the obtained product of polyphenol content more than 90%, tea-polyphenol yield is between 9-11%.
3. a kind of technique extracting tea-polyphenol from big-leaf species in yunnan tealeaves according to claim 1, is characterized in that, the low value adopting the production tea time to produce discards fannings as raw material.
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| CN201410801452.XA CN104497057A (en) | 2014-12-22 | 2014-12-22 | Process for extracting tea polyphenols from Yunan dayezhong tea leaves |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107954968A (en) * | 2017-11-16 | 2018-04-24 | 华南师范大学 | A kind of simple efficient Two Liquid Phases extracting and developing purifying and the method for preparing high-purity tea polypenols |
| CN111840410A (en) * | 2020-07-28 | 2020-10-30 | 上海科德实业有限公司 | Method for extracting high-purity tea polyphenol from tea |
| CN114042114A (en) * | 2021-11-17 | 2022-02-15 | 宜兴市天石饲料有限公司 | Preparation method of tea polyphenol by adopting ethyl acetate extraction |
| CN115024370A (en) * | 2022-06-20 | 2022-09-09 | 云南西草资源开发有限公司 | Method for extracting tea polyphenol from Pu' er tea leaves by fermentation method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1253940A (en) * | 1998-11-12 | 2000-05-24 | 无锡轻工大学 | Method for extracting tea-polyphenol from tea |
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2014
- 2014-12-22 CN CN201410801452.XA patent/CN104497057A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1253940A (en) * | 1998-11-12 | 2000-05-24 | 无锡轻工大学 | Method for extracting tea-polyphenol from tea |
Non-Patent Citations (3)
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| 刘仲华等: "儿茶素提制工艺技术研究", 《湖南农业大学学报》, vol. 23, no. 6, 31 December 1997 (1997-12-31) * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107954968A (en) * | 2017-11-16 | 2018-04-24 | 华南师范大学 | A kind of simple efficient Two Liquid Phases extracting and developing purifying and the method for preparing high-purity tea polypenols |
| CN107954968B (en) * | 2017-11-16 | 2021-04-30 | 华南师范大学 | Simple and efficient double-liquid-phase extraction, separation and purification and method for preparing high-purity tea polyphenol |
| CN111840410A (en) * | 2020-07-28 | 2020-10-30 | 上海科德实业有限公司 | Method for extracting high-purity tea polyphenol from tea |
| CN114042114A (en) * | 2021-11-17 | 2022-02-15 | 宜兴市天石饲料有限公司 | Preparation method of tea polyphenol by adopting ethyl acetate extraction |
| CN115024370A (en) * | 2022-06-20 | 2022-09-09 | 云南西草资源开发有限公司 | Method for extracting tea polyphenol from Pu' er tea leaves by fermentation method |
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Application publication date: 20150408 |