CN102432578A - Method for extracting proanthocyanidins from seabuckthorm seeds - Google Patents
Method for extracting proanthocyanidins from seabuckthorm seeds Download PDFInfo
- Publication number
- CN102432578A CN102432578A CN201110367195XA CN201110367195A CN102432578A CN 102432578 A CN102432578 A CN 102432578A CN 201110367195X A CN201110367195X A CN 201110367195XA CN 201110367195 A CN201110367195 A CN 201110367195A CN 102432578 A CN102432578 A CN 102432578A
- Authority
- CN
- China
- Prior art keywords
- seed
- proanthocyanidins
- seabuckthorm
- extracting
- pycnogenols
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Abstract
The invention relates to a method for extracting proanthocyanidins from seabuckthorm seeds. The method is characterized in that: seabuckthorm seed oil is extracted by employing a supercritical CO2 extraction technology, and seabuckthorm seed proanthocyanidins extract is extracted from seabuckthorm seed meal by employing an ultrasonic method, then the proanthocyanidins in the seabuckthorm seeds is extracted by using a microwave drying technology. The method for extracting the proanthocyanidins from the seabuckthorm seeds comprises the following steps of: extracting the seabuckthorm seed oil by employing the supercritical CO2 extraction technology to obtain the seabuckthorm seed meal; extracting the seabuckthorm seed meal by employing the ultrasonic method at low temperature to obtain a seabuckthorm seed proanthocyanidins ethanol extracting solution, and recovering the ethanol under a negative pressure at low temperature to obtain a proanthocyanidins water solution; and drying the proanthocyanidins water solution by employing vacuum microwave to obtain the seabuckthorm seed proanthocyanidins extract product. According to the method disclosed by the invention, the production process temperature is low, the product with high content of the proanthocyanidins and high quality and without solvent residues is obtained, and the demands of various proanthocyanidins healthcare products and medicament development are satisfied.
Description
Technical field
The present invention relates to the process for extracting of pycnogenols in the seed of Fructus Hippophae
Background technology
Pycnogenols is the general name of the extensive one big type of polyphenolic substance that exists in the vegetable kingdom.20 middle of century, French scientist Maas chief reins in and has at first found pycnogenols.Through nearly 50 years research, people improve constantly the understanding of pycnogenols pharmacological action.In recent years, a host of facts prove that pycnogenols is not only a kind of good antioxidant, and can regulate the crucial biological approach of body, participate in the many redox chain reaction process in the basic vital process.Experiment confirm, oligomeric procyanidolics has direct or indirect prevention, therapeutic action to nearly hundred kinds of damages and disease, has extensive use in fields such as healthcare products, medicine and makeup.
In numerous plants, grape is unfailing research topic always, at present, still is that raw material extracts pycnogenols both at home and abroad with the Semen Vitis viniferae.Research shows that seed of Fructus Hippophae is better pycnogenols source, because its extract contains more dimer, tripolymer, and dimer, the more single aggressiveness of tripolymer and polymer have stronger oxidation-resistance.The Semen Vitis viniferae extract composition is a pycnogenols, and monomer whose is a catechin, and mean polymerisation degree is lower.The sea buckthorn seed proanthocyanidins significantly different from the chemical structure of grape seed proanthocyanidins, mainly for the original prodelphinidin (monomer epigallocatechin), with an average degree of polymerization of 12.8.The effect of seed of Fructus Hippophae pycnogenols inhibited oxidation is better than grape pip procyanidin.
Summary of the invention
Technical problem to be solved by this invention is to unite to use the supercritical CO 2 abstraction technique to extract Seabuckthorm Seed Oil; Its seed dregs of rice adopt ultrasonic method to extract the seed of Fructus Hippophae procyanidin extract, adopt the dry three technology of microwave drying technology to extract the method for the pycnogenols in the sea-buckthorn seed cake.
For addressing the above problem, a kind of method of extracting pycnogenols in the seed of Fructus Hippophae of the present invention comprises the steps:
1. after the seed of Fructus Hippophae removal of impurities fragmentation, adopt supercritical CO
2Abstraction technique obtains the sea-buckthorn seed cake in low temperature (50 ℃) production process after extracting Seabuckthorm Seed Oil, and no solvent residue.
2. adopt ultrasonic method (50 ℃) to extract sea-buckthorn seed cake, get seed of Fructus Hippophae pycnogenols ethanol extract.
3. negative pressure low temperature reclaims edible ethanol, gets the pycnogenols aqueous solution.
4. promptly get the seed of Fructus Hippophae procyanidin extract after adopting vacuum microwave with pycnogenols aqueous solution drying, its procyanidin content can reach 50%.
The present invention compared with prior art has following advantage:
1, extract Seabuckthorm Seed Oil with solvent method, circumfluence method is extracted the pycnogenols in the sea-buckthorn seed cake, and the refining several situation of crude extract of spraying drying pycnogenols extracting solution and resin absorption are compared, and the present invention is an associating use supercritical CO
2Abstraction technique, ultrasonic method extractive technique, vacuum microwave drying technology; Its production process is in more all the time carries out under the low temperature; Avoided the influence of high temperature to the heat-sensitive substance pycnogenols as far as possible, simultaneously, supersound extraction and vacuum microwave drying not only carry out at low temperatures; Also shorten the time of production process greatly, also obviously reduced the loss of pycnogenols.The inventive method has been evaded the toxic action that resin possibly come off and bring fully without the refining crude extract of resin absorption.The real no solvent residue of seed of Fructus Hippophae procyanidin extract, high-content, high-quality that the inventive method obtains.And application for a patent for invention number: the method for 200810176033.6 method and " international sea-buckthorn research and development " the 8th volume the 3rd phase P11 page or leaf September in 2010; Procyanidin content all about 30%, can only adopt further refining its content that improves of resin again in the resulting seed of Fructus Hippophae procyanidin extract crude extract.Increase its production cost undoubtedly, also the risk of toxicity that resin wear brings possibly occur.
2, the present invention adopts vacuum microwave drying pycnogenols extracting solution, temperature low (below 50 ℃), time short (in 2 hours), and the quality product that obtains is fully suitable with cryodesiccated quality.And the spraying drying of application for a patent for invention numbers 200810176033.6, because temperature is high, the pycnogenols of thermo-sensitivity will inevitably significantly sacrificing; And the cryodesiccated time (extracting liquid measure on an equal basis) is more than 20 times of microwave drying time, and its production cost is more than 2.7 times of microwave drying production cost.
Embodiment
Embodiment 1: a kind of method of extracting pycnogenols in the seed of Fructus Hippophae comprises the steps:
1. adopt supercritical CO
2The sea-buckthorn seed cake 1kg of extraction behind the Seabuckthorm Seed Oil extracts 3 times through ultrasonic method down at 30 ℃ with 8L, 30% ethanolic soln, at every turn 0.35 hour extraction time.Merge No. three times extracting solution, cross with 200 order filter clothes and filter filtrating.Negative pressure low temperature reclaims edible ethanol, gets the seed of Fructus Hippophae pycnogenols aqueous solution.
2. get powdery seed of Fructus Hippophae procyanidin extract product 82g after the seed of Fructus Hippophae pycnogenols aqueous solution being adopted the vacuum microwave cryodrying, its procyanidin content is 49.6%.
Wherein: the 24L supercritical CO that sea-buckthorn seed cake adopts Guangzhou light industry to be produced
2Seed oil in the extraction plant extraction seed of Fructus Hippophae, its parameter is extraction temperature 45-50 ℃, extracting pressure is 26-32MPa, extraction time 2.5-3.5h.
Embodiment 2: a kind of method of extracting pycnogenols in the seed of Fructus Hippophae comprises the steps:
1. adopt supercritical CO
2The sea-buckthorn seed cake 10kg of extraction behind the Seabuckthorm Seed Oil extracts 2 times through ultrasonic method down at 50 ℃ with 100L, 80% ethanolic soln, at every turn 0.5 hour extraction time.Merge secondary raffinate, cross with 200 order filter clothes and filter filtrating.Negative pressure low temperature reclaims edible ethanol, gets the seed of Fructus Hippophae pycnogenols aqueous solution.
2. get powdery seed of Fructus Hippophae procyanidin extract product 0.95kg after the seed of Fructus Hippophae pycnogenols aqueous solution being adopted the vacuum microwave cryodrying, its procyanidin content is 50.6%.
Wherein: the 24L supercritical CO that sea-buckthorn seed cake adopts Guangzhou light industry to be produced
2Seed oil in the extraction plant extraction seed of Fructus Hippophae, its parameter is extraction temperature 45-50 ℃, extracting pressure is 26-32MPa, extraction time 2.5-3.5h.
Description of drawings
Accompanying drawing is a process diagram of the present invention.
Claims (3)
1. a method of extracting pycnogenols in the seed of Fructus Hippophae is characterized in that: unite the use supercritical CO
2Abstraction technique extracts Seabuckthorm Seed Oil, and its seed dregs of rice adopt ultrasonic method to extract the seed of Fructus Hippophae procyanidin extract, adopts the dry three technology of microwave drying technology to extract the method for the pycnogenols in the sea-buckthorn seed cake.
2. the seed dregs of rice as claimed in claim 1 adopt ultrasonic method to carry the seed of Fructus Hippophae procyanidin extract, and it is characterized in that: temperature 30-60 ℃, alcohol concn is 30-80%, and solid-liquid ratio is 1: 5～1: 15.
3. the seed of Fructus Hippophae pycnogenols extracting solution behind the employing microwave drying technology dried recovered ethanol as claimed in claim 1 is characterized in that: vacuum microwave drying, temperature 45-55 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110367195.XA CN102432578B (en) | 2011-11-18 | 2011-11-18 | Method for extracting proanthocyanidins from seabuckthorm seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110367195.XA CN102432578B (en) | 2011-11-18 | 2011-11-18 | Method for extracting proanthocyanidins from seabuckthorm seeds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102432578A true CN102432578A (en) | 2012-05-02 |
| CN102432578B CN102432578B (en) | 2015-06-10 |
Family
ID=45980968
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110367195.XA Active CN102432578B (en) | 2011-11-18 | 2011-11-18 | Method for extracting proanthocyanidins from seabuckthorm seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102432578B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102755359A (en) * | 2012-07-19 | 2012-10-31 | 中国科学院研究生院 | Hippophae extract and preparation method and application thereof for inhibiting activity of fatty acid synthase |
| CN104997109A (en) * | 2015-08-06 | 2015-10-28 | 贵州三农楮桃产业开发有限公司 | Broussonetia papyrifera pulp rich in proanthocyanidins as well as preparation method and application of broussonetia papyrifera pulp |
| CN105440003A (en) * | 2016-01-12 | 2016-03-30 | 北京宝得瑞健康产业有限公司 | Method for efficiently extracting procyanidine in sea-buckthorn seeds at low temperature and with low energy consumption |
| CN109133272A (en) * | 2018-08-24 | 2019-01-04 | 青海清华博众生物技术有限公司 | A kind of purification process of plateau ecology hard water |
| CN109593075A (en) * | 2018-09-25 | 2019-04-09 | 宝得瑞（湖北）健康产业有限公司 | A kind of method that room temperature efficient high-pressure difference continous way extracts seabuckthorn seeds procyanidine |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08205818A (en) * | 1995-02-01 | 1996-08-13 | Nonogawa Shoji Kk | Health food and medicine formulated with procyanidin |
| JPH10218769A (en) * | 1997-02-06 | 1998-08-18 | Kikkoman Corp | Antiulcer agent |
| EP1388343A1 (en) * | 2002-08-09 | 2004-02-11 | Eromlife Co., Ltd. | Antioxidant composition and method of preparation thereof |
| CN101386615A (en) * | 2008-11-05 | 2009-03-18 | 中国科学院西北高原生物研究所 | Method for extracting procyanidine from sea-buckthorn seed |
| CN101496581A (en) * | 2008-02-03 | 2009-08-05 | 于珩 | Health-care food for adjusting blood fat |
| CN101701013A (en) * | 2009-11-05 | 2010-05-05 | 中国林业科学研究院林产化学工业研究所 | Method for extracting proanthocyanidin from pine bark by taking water as solvent |
| CN101768621A (en) * | 2010-02-11 | 2010-07-07 | 青海康普生物科技股份有限公司 | Method for preparing sea-buckthorn enzymes from seabuckthorn fruit mud |
-
2011
- 2011-11-18 CN CN201110367195.XA patent/CN102432578B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08205818A (en) * | 1995-02-01 | 1996-08-13 | Nonogawa Shoji Kk | Health food and medicine formulated with procyanidin |
| JPH10218769A (en) * | 1997-02-06 | 1998-08-18 | Kikkoman Corp | Antiulcer agent |
| EP1388343A1 (en) * | 2002-08-09 | 2004-02-11 | Eromlife Co., Ltd. | Antioxidant composition and method of preparation thereof |
| CN101496581A (en) * | 2008-02-03 | 2009-08-05 | 于珩 | Health-care food for adjusting blood fat |
| CN101386615A (en) * | 2008-11-05 | 2009-03-18 | 中国科学院西北高原生物研究所 | Method for extracting procyanidine from sea-buckthorn seed |
| CN101701013A (en) * | 2009-11-05 | 2010-05-05 | 中国林业科学研究院林产化学工业研究所 | Method for extracting proanthocyanidin from pine bark by taking water as solvent |
| CN101768621A (en) * | 2010-02-11 | 2010-07-07 | 青海康普生物科技股份有限公司 | Method for preparing sea-buckthorn enzymes from seabuckthorn fruit mud |
Non-Patent Citations (2)
| Title |
|---|
| 杜学禹等: "加勒比松树皮中原花青素的提取工艺研究", 《生物质化学工程》 * |
| 金海英等: "沙棘籽渣中低聚原花青素提取和精致工艺研究", 《沙棘》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102755359A (en) * | 2012-07-19 | 2012-10-31 | 中国科学院研究生院 | Hippophae extract and preparation method and application thereof for inhibiting activity of fatty acid synthase |
| CN102755359B (en) * | 2012-07-19 | 2014-04-02 | 中国科学院研究生院 | Hippophae extract and preparation method and application thereof for inhibiting activity of fatty acid synthase |
| CN104997109A (en) * | 2015-08-06 | 2015-10-28 | 贵州三农楮桃产业开发有限公司 | Broussonetia papyrifera pulp rich in proanthocyanidins as well as preparation method and application of broussonetia papyrifera pulp |
| CN105440003A (en) * | 2016-01-12 | 2016-03-30 | 北京宝得瑞健康产业有限公司 | Method for efficiently extracting procyanidine in sea-buckthorn seeds at low temperature and with low energy consumption |
| CN105440003B (en) * | 2016-01-12 | 2017-11-17 | 北京宝得瑞健康产业有限公司 | A kind of method of low temperature low-energy-consumption high-efficiency extraction proanthocyanidins from seabuckthorm seeds |
| CN109133272A (en) * | 2018-08-24 | 2019-01-04 | 青海清华博众生物技术有限公司 | A kind of purification process of plateau ecology hard water |
| CN109593075A (en) * | 2018-09-25 | 2019-04-09 | 宝得瑞（湖北）健康产业有限公司 | A kind of method that room temperature efficient high-pressure difference continous way extracts seabuckthorn seeds procyanidine |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102432578B (en) | 2015-06-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106860492B (en) | Preparation method of cannabinol compound | |
| CN102731486B (en) | New method for purifying silymarin | |
| CN102432578B (en) | Method for extracting proanthocyanidins from seabuckthorm seeds | |
| CN103710152A (en) | Method for using supercritical carbon dioxide for extraction of chrysanthemum essential oil | |
| CN105294632B (en) | A kind of industrial method preparing indigo fruit anthocyanidin from indigo fruit | |
| CN104928018A (en) | Method for extracting tea-seed oil by microwave expansion assisted biological enzymolysis | |
| CN102876456A (en) | Method for extracting volatile oil and alkaloid from pepper by using subcritical fluid | |
| CN105440003A (en) | Method for efficiently extracting procyanidine in sea-buckthorn seeds at low temperature and with low energy consumption | |
| CN102061220A (en) | Method for preparing onion functional components by using double-stage extraction based on subcritical water and solvent method | |
| CN103214448A (en) | Method for extracting procyanidine from peanut skin | |
| CN101386615A (en) | Method for extracting procyanidine from sea-buckthorn seed | |
| CN105925363B (en) | A kind of low acid number pine-seed oil and the extracting method of Pinolenic acid | |
| CN106518830A (en) | Method for extracting anthocyanins | |
| CN103146473A (en) | Method for simultaneously extracting Australian tea tree essential oil and flavonoid glycosides | |
| CN102040612A (en) | Method for extracting bergamottin from grapefruit peel | |
| CN101628857B (en) | Production technique for extracting resveratrol from giant knotweed | |
| CN103450702A (en) | Microwave pretreatment method for preparing capsicum red pigment and capsaicin | |
| CN105418724A (en) | Method for extracting highly-pure tea saponin from oil tea cake | |
| CN105294633B (en) | A kind of industrial method preparing cowberry anthocyanidin from cowberry | |
| CN104277909A (en) | Method for extracting high-content sesame lignan sesame oil from sesame | |
| CN101352616A (en) | Method for preparing pine tree bark extract using barrier separation integrated process engineering | |
| CN105524035B (en) | A kind of manufacture method of mulberries anthocyanidin | |
| CN103432205A (en) | Novel method for extracting high-purity lotus leaf flavone | |
| CN107474087A (en) | A kind of method that separation european bird cherry anthocyanin is extracted from european bird cherry pericarp | |
| CN104152262A (en) | Method for co-producing eucommia seed oil, eucommia rubber and eucommia extract by refining eucommia fruits through steam explosion |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| ASS | Succession or assignment of patent right |
Owner name: MA YINGYUAN Effective date: 20120525 Owner name: BEIJING POWDERY FOOD CO., LTD. Free format text: FORMER OWNER: MA YINGYUAN Effective date: 20120525 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 100123 CHAOYANG, BEIJING TO: 101117 TONGZHOU, BEIJING |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20120525 Address after: 101117 Beijing City Lucheng town Tongzhou District City Industrial Zone No. 15 Applicant after: Beijing Powdery Food Co., Ltd. Co-applicant after: Ma Yingyuan Address before: 100123, room 1, unit 5, 102 middle building, Chaoyang District manna garden, Beijing Applicant before: Ma Yingyuan |
|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |