CN103214448A - Method for extracting procyanidine from peanut skin - Google Patents
Method for extracting procyanidine from peanut skin Download PDFInfo
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- CN103214448A CN103214448A CN2013101044500A CN201310104450A CN103214448A CN 103214448 A CN103214448 A CN 103214448A CN 2013101044500 A CN2013101044500 A CN 2013101044500A CN 201310104450 A CN201310104450 A CN 201310104450A CN 103214448 A CN103214448 A CN 103214448A
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Abstract
The invention discloses a method for extracting procyanidine from peanut skin, and relates to the technical field of biological chemical extraction. The method adopts the following technical schemes that the method for extracting procyanidine from peanut skin is special in taking the peanut skin as a material and extracting by a known extraction method. Compared with the prior art, the method has the outstanding substantive characteristics and significant progresses that the method is suitable for industrial production, simple in extraction method, high in efficiency, low in expenses, green and environment-friendly; and the original biological characteristics are maintained.
Description
Technical field
The present invention relates to biochemical industry extractive technique field, relate in particular to a kind of technical field of from Testa arachidis hypogaeae, extracting the method for pycnogenols.
Background technology
Pycnogenols is called for short OPC, is a kind of Vitamin P complex that special molecular structure is arranged, and is the most effective natural antioxidants of free radical in the removing human body of generally acknowledging in the world at present.Be generally the reddish-brown powder, little, the puckery of gas, water-soluble and most organic solvent.Pycnogenols, English name is Oligomeric Proantho Cyanidins(OPC), English another name: 2-(3,4-Dihydroxyphenyl)-2-((2-(3,4-dihydroxyphenyl)-3,4-dihydro-5,7-dihydroxy-2H-1-benzopyran-3-yl) oxy)-3,4-dihydro-2H-1-benzopyran-3,4,5,7-tetrol;
[1]2-(3,4-dihydroxyphenyl)-2-{[2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3,4-dihydro-2H-chromen-3-yl] oxy}-3,4-dihydro-2H-chromene-3,4,5,7-tetrol, be a kind of Vitamin P complex that special molecular structure is arranged, be generally Semen Vitis viniferae extract or French maritime pine bark extract.Pycnogenols (Semen Vitis viniferae extract) is a kind of new and effective antioxidant, and the most potent free-radical scavengers that is so far to be found has very strong activity in vivo.The Green Tea Extract oxidation capacity that experiment showed, OPC is 50 times of vitamin-E, ascorbic 20 times, and absorb rapidly fully, can reach the highest haemoconcentration in oral 20 minutes, metabolic half life is for 7 hours.Structurally, pycnogenols is catechin (catechin) or l-Epicatechol (epicatechin) be combined into by different quantities.The simplest pycnogenols is the dimer of catechin or l-Epicatechol or catechin and l-Epicatechol formation, also has tripolymer, the tetramer etc. in addition until ten aggressiveness, ten pentamers.By the size of the polymerization degree, usually two～pentamer is called oligomer (being called for short OPC), will be called high polymer (being called for short PPC) more than the pentamer.
Pycnogenols is a kind of polyphenols that belongs to, and the interval range of its molecular weight is also bigger, the Vitamin P complex of special molecular structure; Pycnogenols has extremely strong oxidation-resistance, enzyme inhibition activity, anti-mutation activity etc., and therefore, it is used as in anti-oxidant, radioprotective, antitumor and protect the medicine of diseases such as cardiovascular and preventing hypertension to have more widely and use.Having had multiple in the market is the medicine of healthcare products, makeup and the assisting therapy of effective constituent with the pycnogenols, and it has healthyly played positive effect for what promote people.Only the pycnogenols that extracts with Semen Vitis viniferae is an example, CAS number: 84929-27-1,4852-22-6, molecular formula C30H12O6, molecular weight 468.42, procyanidin content are 95%, and the seed of vitaceae grape (latin name VitisviniferaL) extracts, character is the reddish-brown powder, have faint scent, astringent taste is arranged, its pharmacological action: protect cardiovascular and the preventing hypertension effect, antitumor action, radiation resistance; Be mainly used in aspect medicine and the healthcare products: anti-oxidant, remove free radical, beautify skin, vision protection, improve anoxic, alleviating fatigue. alleviate the generation of anaphylaxis, prevention cardiovascular and cerebrovascular diseases, promote that people's is healthy.
Pycnogenols generally is present in the skin of fruit and the wooden real part of plant, the extraction of existing pycnogenols, serve as the main raw material that extracts still with Cortex Pini and Semen Vitis viniferae, its extracting method commonly used be milling process, organic solvent extractionprocess,, water extract-alcohol precipitation-resin adsorption method, supercritical CO 2 extraction process etc.The extraction raw material of just present pycnogenols, efficient and the productive rate that extracting method all influences its extraction.Therefore, exploitation is extracted the new raw material of pycnogenols and is sought the new extracting method of pycnogenols, and the market requirement that satisfies pycnogenols has become the problem that those skilled in the art need to be resolved hurrily.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, and a kind of suitability for industrialized production, new extracting method of being applicable to be provided, efficient height, expense be low, keep method original biological nature, environmental protection, extract pycnogenols from Testa arachidis hypogaeae.
For solving above-mentioned technical problem, the present invention adopts to implement following technical scheme: a kind of method of extracting pycnogenols from Testa arachidis hypogaeae, its special character is to be raw material with the Testa arachidis hypogaeae, extracts with known extracting method.
For further solving above-mentioned technical problem, the preferred version of technique scheme is:
Extracting method of the present invention is to be raw material with the Testa arachidis hypogaeae, after crushed, the water mixing homogenate that adds 1:10-25 times of weight, residue is removed in press filtration, nanofiltration I molecular weight cut-off is the above materials of 2500 dalton, getting permeate is high polymer pycnogenols more than 1000 dalton through nanofiltration II molecular weight cut-off, getting permeate again is Procyanidcic Oligomers more than 500 dalton through nanofiltration III molecular weight cut-off, further getting permeate again is monomer pycnogenols below 250 dalton through nanofiltration IV molecular weight cut-off, gets each trapped substance respectively and is drying to obtain different pure product.
Described known extracting method is to have microwave assisting method to extract; Also can be that ultrasonic auxiliary law extracts; It also can be water extract-alcohol precipitation-resin adsorption method; Can also extract with ultra supercritical CO2 extraction process.
In the aforesaid step, described mixing homogenate is to carry out in the high-speed homogenization machine high-speed homogenization 0.5-3 hour.
In the aforesaid step, described press filtration is to carry out press filtration with mixing homogenate under the air pressure of 0.5-1MPA, crosses the 300-700 mesh sieve.
In the aforesaid step, the above material of described nanofiltration I molecular weight cut-off 2500 dalton be with the filtrate after the press filtration through the rolling nanofiltration device, remove material more than molecular weight 2500 dalton adopting the cross flow filter method to carry out nanofiltration under the pressure of 0.01-0.05 MPA.
In the aforesaid step, described nanofiltration II be with the permeate of nanofiltration I in can the rolling nanofiltration device more than molecular weight cut-off 1000 dalton, adopting the cross flow filter method to carry out pure product of the high polymer pycnogenols aqueous solution more than nanofiltration molecular weight cut-off 1000 dalton under the pressure of 0.01-0.05 MPA.
In the aforesaid step, described nanofiltration III be with the permeate of nanofiltration II molecular weight cut-off being in the nanofiltration device of the above Procyanidcic Oligomers aqueous solution of 500 dalton, under the pressure of 0.01-0.15MPA, carry out nanofiltration and get the pure product of the Procyanidcic Oligomers aqueous solution.
In the aforesaid step, described nanofiltration IV is can molecular weight cut-off be in the nanofiltration device of the above monomer pycnogenols of the 250 dalton aqueous solution with the permeate of nanofiltration III, and carrying out nanofiltration under the pressure of 0.01-0.15MPA, to get trapped substance be the pure product of the Procyanidcic Oligomers aqueous solution.
In the aforesaid step, described drying is spraying drying or lyophilize.
The present invention compared with prior art has following outstanding substantive distinguishing features and obvious improvement:
The extracting method of first pycnogenols of the present invention is to adopt Testa arachidis hypogaeae as extracting raw material, the Testa arachidis hypogaeae tankage had both been utilized, realized turning waste into wealth, reduced the material cost of pycnogenols again, for the price that reduces existing pycnogenols, benefit the people, improve the business economic benefit and created precondition;
It two is that the extracting method of pycnogenols of the present invention is by after pulverizing and adding entry or aqueous ethanol with Testa arachidis hypogaeae, can obtain the pure product of pycnogenols through homogenate, press filtration, nanofiltration I, nanofiltration II, nanofiltration III, nanofiltration IV, drying, its whole leaching process has only water as solvent, thereby the pycnogenols that method of the present invention is extracted not only has and is all satisfying the particular requirement of pharmaceutical industries aspect security, price and the purity, and has reached the outstanding substantive distinguishing features that keeps original biological nature, environmental protection;
It three is that the extracting method of pycnogenols of the present invention is that the control process of can be directly carrying out mechanize by equipment is implemented, need not to operate by hand, parameters of technique process is realized by equipment fully, production process is moved continuously, realized technological process mechanize, the course of conveying automatization, reach industrial production requirement fully, this makes method of the present invention not only have the extracting method novelty, simple to operate, reliably, the efficient height, the outstanding substantive distinguishing features that productive expense is low, and have condition and a requirement that meets industrialized mass fully, provide a new technical scheme for satisfying society to the growing demand of anthocyanidin, thereby made the present invention compared with prior art have its outstanding substantive distinguishing features and obvious improvement.
Embodiment
Describe a kind of specific implementation process of from Testa arachidis hypogaeae, extracting the method for pycnogenols of the present invention in detail below in conjunction with embodiment.
Embodiment 1:
A kind of method of from Testa arachidis hypogaeae, extracting pycnogenols of the embodiment of the invention 1, it comprises the steps: to get Testa arachidis hypogaeae weigh 10KG, pulverizing successively, the water mixing homogenate that adds 1:10 times of weight, under the air pressure of 0.5-0.6MPA, carry out press filtration with mixing homogenate, cross 300 mesh sieves; Extracting the method for pycnogenols can finish with known microwave assisting method.
Embodiment 2:
A kind of method of from Testa arachidis hypogaeae, extracting pycnogenols of the embodiment of the invention 2, it comprises the steps: to get Testa arachidis hypogaeae weigh 10KG, pulverizing successively, the water mixing homogenate that adds 1:15 times of weight, under the air pressure of 0.6-0.7MPA, carry out press filtration with mixing homogenate, cross 400 mesh sieves, can finish with the method that known ultrasonic auxiliary law extracts pycnogenols.
Embodiment 3:
A kind of method of from Testa arachidis hypogaeae, extracting pycnogenols of the embodiment of the invention 3, it comprises the steps: to get Testa arachidis hypogaeae weigh 20KG, pulverizing successively, the water mixing homogenate that adds 1:20 times of weight, under the air pressure of 0.8MPA, carry out press filtration with mixing homogenate, cross 600 mesh sieves, extract the method for pycnogenols with known water extract-alcohol precipitation-resin adsorption method and can finish.
Embodiment 4:
A kind of method of from Testa arachidis hypogaeae, extracting pycnogenols of the embodiment of the invention 4, it comprises the steps: to get Testa arachidis hypogaeae weigh 20KG, pulverizing successively, the water mixing homogenate that adds 1:25 times of weight, under the air pressure of 0.9MPA, carry out press filtration with mixing homogenate, cross 700 mesh sieves, extract the method for pycnogenols with known supercritical CO 2 extraction process and can finish.
Embodiment 5:
The embodiment of the invention 5 described extracting method are to be raw material with the Testa arachidis hypogaeae, after crushed, the water mixing homogenate that adds 1:10-25 times of weight, residue is removed in press filtration, nanofiltration I molecular weight cut-off is the above materials of 2500 dalton, getting permeate is high polymer pycnogenols more than 1000 dalton through nanofiltration II molecular weight cut-off, getting permeate again is Procyanidcic Oligomers more than 500 dalton through nanofiltration III molecular weight cut-off, further getting permeate again is monomer pycnogenols below 250 dalton through nanofiltration IV molecular weight cut-off, gets each trapped substance respectively and is drying to obtain different pure product.Specific as follows:
1. get the pure water mixing homogenate that Testa arachidis hypogaeae after the pulverizing is starched 10 ㎏, added 1:10 times of weight, mixing homogenate is to carry out high-speed homogenization 0.5 hour in the high-speed homogenization machine;
2. residue is removed in press filtration, is above-mentioned mixing homogenate is carried out press filtration in the basket-hanging air pressure filter under the air pressure of 0.5MPA, crosses 300 eye mesh screens;
3. nanofiltration I molecular weight cut-off be the above material of 2500 dalton be with the filtrate above-mentioned screen cloth under through the rolling nanofiltration device more than molecular weight cut-off 2500 dalton, carry out material more than nanofiltration molecular weight cut-off 2500 dalton in employing cross flow filter method under the pressure of 0.01 MPA.
4. described nanofiltration II be the above material of molecular weight cut-off 1000 dalton be with above-mentioned permeate in can the rolling nanofiltration device more than molecular weight cut-off 1000 dalton, adopting the cross flow filter method to carry out pure product of the high polymer pycnogenols aqueous solution more than nanofiltration molecular weight cut-off 1000 dalton under the pressure of 0.01-0.02MPA.
5. described nanofiltration III be with above-mentioned permeate can molecular weight cut-off be in the nanofiltration device of the above Procyanidcic Oligomers aqueous solution of 500 dalton, under the pressure of 0.01-0.03MPA, carry out nanofiltration and get the pure product of the Procyanidcic Oligomers aqueous solution.
6. described nanofiltration IV be with above-mentioned permeate can molecular weight cut-off be in the nanofiltration device of the above monomer pycnogenols of the 250 dalton aqueous solution, under the pressure of 0.01-0.03MPA, carry out nanofiltration, trapped substance be the pure product of the Procyanidcic Oligomers aqueous solution.
7. be drying to obtain pure product, the above-mentioned pure product of the pycnogenols aqueous solution of respectively holding back gained by spraying drying, can be obtained the different pure product of powdery pycnogenols.
It more than is a kind of specific implementation process of from Testa arachidis hypogaeae, extracting the method for pycnogenols of the embodiment of the invention 5.
Embodiment 6:
The embodiment of the invention 6 described extracting method are to be raw material with the Testa arachidis hypogaeae, after crushed, the water mixing homogenate that adds 1:10-25 times of weight, residue is removed in press filtration, nanofiltration I molecular weight cut-off is the above materials of 2500 dalton, getting permeate is high polymer pycnogenols more than 1000 dalton through nanofiltration II molecular weight cut-off, getting permeate again is Procyanidcic Oligomers more than 500 dalton through nanofiltration III molecular weight cut-off, further getting permeate again is monomer pycnogenols below 250 dalton through nanofiltration IV molecular weight cut-off, gets each trapped substance respectively and is drying to obtain different pure product.Specific as follows:
1. get grape leftover bits and pieces slip 10 ㎏ after the pulverizing, add the tap water mixing homogenate of 1:15 times of weight, mixing homogenate is to carry out high-speed homogenization 1 hour in the high-speed homogenization machine;
2. residue is removed in press filtration, is above-mentioned mixing homogenate is carried out press filtration in the basket-hanging air pressure filter under the air pressure of 0.6MPA, crosses 400 eye mesh screens;
3. nanofiltration I molecular weight cut-off be the above material of 2500 dalton be with the filtrate above-mentioned screen cloth under through the rolling nanofiltration device more than molecular weight cut-off 2500 dalton, carry out material more than nanofiltration molecular weight cut-off 2500 dalton in employing cross flow filter method under the pressure of 0.02 MPA.
4. described nanofiltration II be the above material of molecular weight cut-off 1000 dalton be with above-mentioned permeate in can the rolling nanofiltration device more than molecular weight cut-off 1000 dalton, adopting the cross flow filter method to carry out pure product of the high polymer pycnogenols aqueous solution more than nanofiltration molecular weight cut-off 1000 dalton under the pressure of 0.02-0.03MPA.
5. described nanofiltration III be with above-mentioned permeate can molecular weight cut-off be in the nanofiltration device of the above Procyanidcic Oligomers aqueous solution of 500 dalton, under the pressure of 0.04-0.06MPA, carry out nanofiltration and get the pure product of the Procyanidcic Oligomers aqueous solution.
6. described nanofiltration IV be with above-mentioned permeate can molecular weight cut-off be in the nanofiltration device of the above monomer pycnogenols of the 250 dalton aqueous solution, under the pressure of 0.04-0.06MPA, carry out nanofiltration, trapped substance be the pure product of the Procyanidcic Oligomers aqueous solution.
7. be drying to obtain pure product, the pure product of the above-mentioned pycnogenols aqueous solution are promptly got the pycnogenols pulvis by vacuum lyophilization.
It more than is a kind of specific implementation process of from Testa arachidis hypogaeae, extracting the method for pycnogenols of the embodiment of the invention 6.
Embodiment 7:
The embodiment of the invention 7 described extracting method are to be raw material with the Testa arachidis hypogaeae, after crushed, the water mixing homogenate that adds 1:10-25 times of weight, residue is removed in press filtration, nanofiltration I molecular weight cut-off is the above materials of 2500 dalton, getting permeate is high polymer pycnogenols more than 1000 dalton through nanofiltration II molecular weight cut-off, getting permeate again is Procyanidcic Oligomers more than 500 dalton through nanofiltration III molecular weight cut-off, further getting permeate again is monomer pycnogenols below 250 dalton through nanofiltration IV molecular weight cut-off, gets each trapped substance respectively and is drying to obtain different pure product.Specific as follows:
1. get Semen Vitis viniferae 10 ㎏ after the pulverizing, add the distilled water mixing homogenate of 1:20 times of weight, mixing homogenate is to carry out high-speed homogenization 1.5 hours in the high-speed homogenization machine;
2. residue is removed in press filtration, is above-mentioned mixing homogenate is carried out press filtration in the basket-hanging air pressure filter under the air pressure of 0.8MPA, crosses 500 eye mesh screens;
3. nanofiltration I molecular weight cut-off be the above material of 2500 dalton be with the filtrate above-mentioned screen cloth under through the rolling nanofiltration device more than molecular weight cut-off 2500 dalton, carry out material more than nanofiltration molecular weight cut-off 2500 dalton in employing cross flow filter method under the pressure of 0.03 MPA.
4. described nanofiltration II be the above material of molecular weight cut-off 1000 dalton be with above-mentioned permeate in can the rolling nanofiltration device more than molecular weight cut-off 1000 dalton, adopting the cross flow filter method to carry out pure product of the high polymer pycnogenols aqueous solution more than nanofiltration molecular weight cut-off 1000 dalton under the pressure of 0.03-0.04MPA.
5. described nanofiltration III be with above-mentioned permeate can molecular weight cut-off be in the nanofiltration device of the above Procyanidcic Oligomers aqueous solution of 500 dalton, under the pressure of 0.07-0.09MPA, carry out nanofiltration and get the pure product of the Procyanidcic Oligomers aqueous solution.
6. described nanofiltration IV be with above-mentioned permeate can molecular weight cut-off be in the nanofiltration device of the above monomer pycnogenols of the 250 dalton aqueous solution, under the pressure of 0.07-0.09MPA, carry out nanofiltration, trapped substance be the pure product of the Procyanidcic Oligomers aqueous solution.
7. be drying to obtain pure product, the pure product of the above-mentioned pycnogenols aqueous solution are promptly got the pycnogenols pulvis by vacuum lyophilization.
It more than is a kind of specific implementation process of from Testa arachidis hypogaeae, extracting the method for pycnogenols of the embodiment of the invention 7.
Embodiment 8:
The embodiment of the invention 8 described extracting method are to be raw material with the Testa arachidis hypogaeae, after crushed, the water mixing homogenate that adds 1:10-25 times of weight, residue is removed in press filtration, nanofiltration I molecular weight cut-off is the above materials of 2500 dalton, getting permeate is high polymer pycnogenols more than 1000 dalton through nanofiltration II molecular weight cut-off, getting permeate again is Procyanidcic Oligomers more than 500 dalton through nanofiltration III molecular weight cut-off, further getting permeate again is monomer pycnogenols below 250 dalton through nanofiltration IV molecular weight cut-off, gets each trapped substance respectively and is drying to obtain different pure product.Specific as follows:
1. get the aqueous ethanol mixing homogenate of trester dry product mixture 10 ㎏, 1:23 times of weight of adding, the ethanol content of aqueous ethanol is 60%, and mixing homogenate is to carry out high-speed homogenization 2 hours in the high-speed homogenization machine;
2. residue is removed in press filtration, is above-mentioned mixing homogenate is carried out press filtration in the basket-hanging air pressure filter under the air pressure of 0.9MPA, crosses 600 eye mesh screens;
3. nanofiltration I molecular weight cut-off be the above material of 2500 dalton be with the filtrate above-mentioned screen cloth under through the rolling nanofiltration device more than molecular weight cut-off 2500 dalton, carry out material more than nanofiltration molecular weight cut-off 2500 dalton in employing cross flow filter method under the pressure of 0.04 MPA.
4. described nanofiltration II be the above material of molecular weight cut-off 1000 dalton be with above-mentioned permeate in can the rolling nanofiltration device more than molecular weight cut-off 1000 dalton, adopting the cross flow filter method to carry out pure product of the high polymer pycnogenols aqueous solution more than nanofiltration molecular weight cut-off 1000 dalton under the pressure of 0.04-0.05MPA.
5. described nanofiltration III be with above-mentioned permeate can molecular weight cut-off be in the nanofiltration device of the above Procyanidcic Oligomers aqueous solution of 500 dalton, under the pressure of 0.10-0.12MPA, carry out nanofiltration and get the pure product of the Procyanidcic Oligomers aqueous solution.
6. described nanofiltration IV be with above-mentioned permeate can molecular weight cut-off be in the nanofiltration device of the above monomer pycnogenols of the 250 dalton aqueous solution, under the pressure of 0.10-0.12MPA, carry out nanofiltration, trapped substance be the pure product of the Procyanidcic Oligomers aqueous solution.
7. be drying to obtain pure product, the pure product of the above-mentioned pycnogenols aqueous solution by spraying drying, are got the pycnogenols pulvis.
It more than is a kind of specific implementation process of from Testa arachidis hypogaeae, extracting the method for pycnogenols of the embodiment of the invention 8.
Embodiment 9:
The embodiment of the invention 9 described extracting method are to be raw material with the Testa arachidis hypogaeae, after crushed, the water mixing homogenate that adds 1:10-25 times of weight, residue is removed in press filtration, nanofiltration I molecular weight cut-off is the above materials of 2500 dalton, getting permeate is high polymer pycnogenols more than 1000 dalton through nanofiltration II molecular weight cut-off, getting permeate again is Procyanidcic Oligomers more than 500 dalton through nanofiltration III molecular weight cut-off, further getting permeate again is monomer pycnogenols below 250 dalton through nanofiltration IV molecular weight cut-off, gets each trapped substance respectively and is drying to obtain different pure product.Specific as follows:
1. get the aqueous ethanol mixing homogenate of trester dry product mixture 10 ㎏, 1:25 times of weight of adding, the ethanol content of aqueous ethanol is 70%, and mixing homogenate is to carry out high-speed homogenization 3 hours in the high-speed homogenization machine;
2. residue is removed in press filtration, is above-mentioned mixing homogenate is carried out press filtration in the basket-hanging air pressure filter under the air pressure of 1MPA, crosses 700 eye mesh screens;
3. nanofiltration I molecular weight cut-off be the above material of 2500 dalton be with the filtrate above-mentioned screen cloth under through the rolling nanofiltration device more than molecular weight cut-off 2500 dalton, carry out material more than nanofiltration molecular weight cut-off 2500 dalton in employing cross flow filter method under the pressure of 0.05 MPA.
4. described nanofiltration II be the above material of molecular weight cut-off 1000 dalton be with above-mentioned permeate in can the rolling nanofiltration device more than molecular weight cut-off 1000 dalton, adopting the cross flow filter method to carry out pure product of the high polymer pycnogenols aqueous solution more than nanofiltration molecular weight cut-off 1000 dalton under the pressure of 0.04-0.05MPA.
5. described nanofiltration III be with above-mentioned permeate can molecular weight cut-off be in the nanofiltration device of the above Procyanidcic Oligomers aqueous solution of 500 dalton, under the pressure of 0.13-0.15MPA, carry out nanofiltration and get the pure product of the Procyanidcic Oligomers aqueous solution.
6. described nanofiltration IV be with above-mentioned permeate can molecular weight cut-off be in the nanofiltration device of the above monomer pycnogenols of the 250 dalton aqueous solution, under the pressure of 0.13-0.15MPA, carry out nanofiltration, trapped substance be the pure product of the Procyanidcic Oligomers aqueous solution.
7. be drying to obtain pure product, the pure product of the above-mentioned pycnogenols aqueous solution by vacuum lyophilization, are got the pycnogenols pulvis.
It more than is a kind of specific implementation process of from Testa arachidis hypogaeae, extracting the method for pycnogenols of the embodiment of the invention 9.
Claims (10)
1.. a method of extracting pycnogenols from Testa arachidis hypogaeae is characterized in that with the Testa arachidis hypogaeae being raw material, extracts with known extracting method.
2. the method for from Testa arachidis hypogaeae, extracting pycnogenols according to claim 1, it is characterized in that described extracting method is is raw material with the Testa arachidis hypogaeae, after crushed, the water mixing homogenate that adds 1:10-25 times of weight, residue is removed in press filtration, nanofiltration I molecular weight cut-off is the above materials of 2500 dalton, getting permeate is high polymer pycnogenols more than 1000 dalton through nanofiltration II molecular weight cut-off, getting permeate again is Procyanidcic Oligomers more than 500 dalton through nanofiltration III molecular weight cut-off, further getting permeate again is Procyanidcic Oligomers below 250 dalton through nanofiltration IV molecular weight cut-off, gets each trapped substance respectively and is drying to obtain different pure product.
3. the method for extracting pycnogenols from Testa arachidis hypogaeae according to claim 1 is characterized in that described known extracting method is: microwave assisting method, ultrasonic auxiliary law, water extract-alcohol precipitation-resin adsorption method or supercritical CO 2 extraction process.
4. the method for extracting pycnogenols from Testa arachidis hypogaeae according to claim 1 and 2 is characterized in that described mixing homogenate is to carry out high-speed homogenization 0.5-3 hour in the high-speed homogenization machine.
5. the method for extracting pycnogenols from Testa arachidis hypogaeae according to claim 1 and 2 is characterized in that described press filtration is to carry out press filtration with mixing homogenate under the air pressure of 0.5-1MPA, crosses the 300-700 mesh sieve.
6. the method for from Testa arachidis hypogaeae, extracting pycnogenols according to claim 2, it is characterized in that the above material of described nanofiltration I molecular weight cut-off 2500 dalton be with the filtrate after the press filtration through the rolling nanofiltration device, remove material more than molecular weight 2500 dalton adopting the cross flow filter method to carry out nanofiltration under the pressure of 0.01-0.05 MPA.
7. the method for from Testa arachidis hypogaeae, extracting pycnogenols according to claim 2, it is characterized in that described nanofiltration II be with the permeate of nanofiltration I in can the rolling nanofiltration device more than molecular weight cut-off 1000 dalton, adopting the cross flow filter method to carry out pure product of the high polymer pycnogenols aqueous solution more than nanofiltration molecular weight cut-off 1000 dalton under the pressure of 0.01-0.05 MPA.
8. the method for from Testa arachidis hypogaeae, extracting pycnogenols according to claim 2, it is characterized in that described nanofiltration III be with the permeate of nanofiltration II molecular weight cut-off being in the nanofiltration device of the above Procyanidcic Oligomers aqueous solution of 500 dalton, under the pressure of 0.01-0.15MPA, carry out nanofiltration and get the pure product of the Procyanidcic Oligomers aqueous solution.
9. the method for from Testa arachidis hypogaeae, extracting pycnogenols according to claim 2, it is characterized in that described nanofiltration IV is can molecular weight cut-off be in the nanofiltration device of the above monomer pycnogenols of the 250 dalton aqueous solution with the permeate of nanofiltration III, carrying out nanofiltration under the pressure of 0.01-0.15MPA, to get trapped substance be the pure product of the Procyanidcic Oligomers aqueous solution.
10. the method for extracting pycnogenols from Testa arachidis hypogaeae according to claim 1 and 2 is characterized in that described drying is spraying drying or lyophilize.
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| CN104803960A (en) * | 2015-02-27 | 2015-07-29 | 云南民族大学 | Method for extracting and separating procyanidine of hawthorn fruit through low-temperature aqueous two phase cooling |
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| CN107549811A (en) * | 2017-10-13 | 2018-01-09 | 山东金胜粮油集团有限公司 | A kind of peanut protein rich in OPC reconstitutes powder and preparation method thereof |
| CN109207548A (en) * | 2018-09-06 | 2019-01-15 | 山东省花生研究所 | A kind of peanut coat oligomeric proanthocyanidins, preparation method and application |
| CN111518069A (en) * | 2020-05-29 | 2020-08-11 | 禹州市合同泰药业有限公司 | Method for extracting anthocyanin from mature fresh fruits of edible fungus vegetables |
| CN115073411A (en) * | 2022-07-25 | 2022-09-20 | 山东金胜粮油食品有限公司 | Method for co-producing peanut red skin procyanidine and dietary fiber and application |
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| CN103880805B (en) * | 2014-04-08 | 2016-02-24 | 山东金胜粮油集团有限公司 | A kind of technique extracting pycnogenols from Testa arachidis hypogaeae |
| CN104803960A (en) * | 2015-02-27 | 2015-07-29 | 云南民族大学 | Method for extracting and separating procyanidine of hawthorn fruit through low-temperature aqueous two phase cooling |
| CN104803960B (en) * | 2015-02-27 | 2017-09-22 | 云南民族大学 | A kind of method that separation FRUCTUS CRATAEGI OPC is extracted in low temperature aqueous two-phase cooling |
| CN106083799A (en) * | 2016-07-31 | 2016-11-09 | 江南大学 | A kind of method preparing different purity and the procyanidin without aflatoxin |
| CN106083799B (en) * | 2016-07-31 | 2018-08-14 | 江南大学 | A method of preparing different purity and the procyanidine without aflatoxin |
| CN107549811A (en) * | 2017-10-13 | 2018-01-09 | 山东金胜粮油集团有限公司 | A kind of peanut protein rich in OPC reconstitutes powder and preparation method thereof |
| CN109207548A (en) * | 2018-09-06 | 2019-01-15 | 山东省花生研究所 | A kind of peanut coat oligomeric proanthocyanidins, preparation method and application |
| CN109207548B (en) * | 2018-09-06 | 2020-10-30 | 山东省花生研究所 | Peanut coat oligomeric proanthocyanidin, and preparation method and application thereof |
| CN111518069A (en) * | 2020-05-29 | 2020-08-11 | 禹州市合同泰药业有限公司 | Method for extracting anthocyanin from mature fresh fruits of edible fungus vegetables |
| CN115073411A (en) * | 2022-07-25 | 2022-09-20 | 山东金胜粮油食品有限公司 | Method for co-producing peanut red skin procyanidine and dietary fiber and application |
| CN115073411B (en) * | 2022-07-25 | 2023-09-15 | 山东金胜粮油食品有限公司 | Peanut skin procyanidine and dietary fiber co-production method and application |
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