CN102657199A - Sterilization composition containing difenodiconazole and pyraclostrobin - Google Patents
Sterilization composition containing difenodiconazole and pyraclostrobin Download PDFInfo
- Publication number
- CN102657199A CN102657199A CN2012101414683A CN201210141468A CN102657199A CN 102657199 A CN102657199 A CN 102657199A CN 2012101414683 A CN2012101414683 A CN 2012101414683A CN 201210141468 A CN201210141468 A CN 201210141468A CN 102657199 A CN102657199 A CN 102657199A
- Authority
- CN
- China
- Prior art keywords
- bactericide
- bactericidal composition
- pyraclostrobin
- powdery mildew
- anthracnose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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Abstract
The invention relates to a pesticide composition and particularly relates to a sterilization composition which is used for controlling powdery mildew, anthracnose, late blight, brown blotch and banded sclerotial blight in agriculture. The sterilization composition contains active ingredients including difenodiconazole and pyraclostrobin. Compared with the single preparation, the sterilization composition has an obvious synergistic effect on agricultural diseases and is beneficial to overcoming and delaying the production of bacteria resistance.
Description
[technical field]
The present invention relates to a kind of composition pesticide, be specifically related to a kind of bactericidal composition, relate to a kind of bactericidal composition that is used for preventing and treating on the agricultural powdery mildew, anthracnose, late blight, brown spot and banded sclerotial blight especially.
[background technology]
Powdery mildew and anthracnose are one type of very important diseases on the crops such as melon and fruit, vegetables, usually cause the significant loss of crop.Late blight is one of main disease on tomato and the potato.By the cercospora brown spot of peanut that Deuteromycotina fungi Cercospora arachidicola Hori causes, the peanut leaf of causing harm, petiole and stem stalk.Banded sclerotial blight is the main disease on paddy rice, the wheat, and generation throughout the year is more serious, main harm leaf sheath and blade.
Pyraclostrobin (English common name: Pyraclostrobin), have another name called pyraclostrobin, chemical name: N-{ 2-(1-(4-chlorphenyl)-1H-pyrazole-3-yl oxygen methyl) phenyl } (N one methoxyl group) methyl carbamate.It has following structural formula:
This bactericide is by the methoxy acrylic bactericide that has the pyrazoles structure concurrently of BASF Aktiengesellschaft's exploitation, can prevent and treat effectively by the fungus-caused crop pest of Ascomycetes, Basidiomycetes, Fungi Imperfecti and Oomycete.The mechanism of action of this bactericide is the mitochondrial respiratory inhibitor.Promptly the electronics transmission suppresses the mitochondrial respiratory effect between cytochrome b and cl through stoping, and makes mitochondria can not produce and provide cell eubolism energy needed (ATP), finally causes cell death.Have protective effect, therapeutic action, interior suction conductivity standing grain resistance of rainwater washing against performance, and range of application is wider.Though pyraclostrobin is control crop powdery mildew, anthracnose, late blight, brown spot and banded sclerotial blight; Especially the new and effective medicament of powdery mildew and anthracnose, however the pyraclostrobin action site is single; If long-term single use; Unavoidably can produce resistance, and this reagent cost is high, difficulty is bigger in plant protection is promoted.Through carrying out composite reducing production costs, delay pesticide resistance and produce with other effective bactericide.
The two azoles of phenylate (English common name: Difenodiconazole), chemical name: 2-[2-chloro-4-(4-chlorobenzene oxygen) phenyl]-1, two (1H-1,2,4-the triazol-1-yl)-2-propyl alcohol of 3-.Application number is chemical structural formula, preparation method and the sterilizing use that discloses the two azoles of phenylate in 200610140836.7 the one Chinese patent application, at this this patent application is incorporated herein by reference in full.The two azoles of phenylate have following structural formula:
[summary of the invention]
Problem such as the objective of the invention is to overcome that single bactericide is single to the agricultural disease preventive and therapeutic effect, the lasting period is short, provide a kind of bactericidal effect good, delay pesticide resistance and the low bactericidal composition of drug cost.
Another object of the present invention provides the purposes that said bactericidal composition is used to prevent and treat the various agricultural disease, especially for the purposes of preventing and treating powdery mildew, anthracnose, late blight, brown spot and banded sclerotial blight.
The present invention realizes through following technical proposals:
The present invention relates to a kind of bactericidal composition, it is characterized in that, this bactericidal composition contains following active component A and B:
A, have the two azoles of phenylate of following structural formula;
B, pyraclostrobin; Wherein the weight ratio of component A and B component is 100: 1～1: 100.
Preferably, the weight ratio of component A and B component is 10: 1～1: 10 in this bactericidal composition.
More preferably, the weight ratio of component A and B component is 5: 1～1: 5 in this bactericidal composition.
The present invention relates to a kind of bactericide, it is characterized in that, this bactericide contains the aforesaid bactericidal composition of 0.1～90 weight %, and surplus is acceptable carrier and an auxiliary agent on the agricultural chemicals.
Preferably, this bactericide contains the aforesaid bactericidal composition of 3～80 weight %.
More preferably, this bactericide contains the aforesaid bactericidal composition of 15～70 weight %.
Except as otherwise noted, " % " involved in the present invention is " percentage by weight ".
Bactericidal composition of the present invention can be used for preventing and treating the various agricultural disease; Particularly powdery mildew, anthracnose, late blight, brown spot and banded sclerotial blight, preferably, powdery mildew is powdery mildew of cucumber and wheat powdery mildew; Anthracnose is cucumber anthracnose; Late blight is a potato late blight, and brown spot is the cercospora brown spot of peanut, and banded sclerotial blight is a rice sheath blight disease.
Bactericide involved in the present invention can be mixed with agricultural and go up acceptable forms, and preferred dosage form is water dispersible granules, suspending agent, wetting powder, microemulsion or aqueous emulsion.Said formulation adopts the method that well known to a person skilled in the art to process, prepare.
With regard to water dispersible granules, those skilled in the art use following main auxiliary agent, adopt conventional method to prepare: dispersant is selected for use from polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate; Wetting agent is selected from polyoxyethylene alcohol, alkyl sulfate, alkylsulfonate, naphthalene sulfonate; Disintegrant is selected from ammonium sulfate, urea, sucrose, glucose, citric acid, succinic acid, sodium bicarbonate; Binding agent is selected from diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose and microcrystalline cellulose class; Filler is selected from diatomite, kaolin, white carbon, fine particle calcium carbonate, sepiolite, talcum powder, attapulgite, potter's clay etc.
With regard to wetting powder, those skilled in the art use following main auxiliary agent, adopt conventional method to prepare: dispersant is selected from polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate; Wetting agent is selected from alkyl sulfate, alkylsulfonate, naphthalene sulfonate; Filler is selected from diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay etc.
With regard to suspending agent; Those skilled in the art use following main auxiliary agent, adopt conventional method to prepare: dispersant is selected from polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate, TERSPERSE 2425 (U.S. Huntsman Corporation produces, the alkyl naphthalene sulfonic acid salt); Alkyl phenol formaldehyde resin polyoxyethylene ether), farming breast 2201, this dish-60# (common name: sorbitan monostearate), emulsifier T-60 (common name: the sorbitan monostearate APEO), agricultural newborn 1601# (common name: phenethyl phenol polyoxyethylene poly-oxygen propylene aether), TERSPERSE 4894 (U.S. Huntsman Corporation product) emulsifier is selected from Nongru-700 # (common name:; Wetting agent is selected from alkylphenol-polyethenoxy and gathers basic ether formaldehyde condensation products sulphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, TERSPERSE2500 (U.S. Huntsman Corporation product); Thickener is selected from xanthans, aluminium-magnesium silicate, bentonite; Preservative is selected from benzoic acid, Sodium Benzoate; Antifoaming agent is silicone based antifoaming agent; Antifreezing agent is selected from ethylene glycol, propane diols, glycerine, urea, inorganic salts such as sodium chloride.
With regard to aqueous emulsion, those skilled in the art use following main auxiliary agent, adopt conventional method to prepare: emulsifier is selected from NPE (EO=10) phosphate, triphen ethyl-phenol polyoxyethylene ether phosphate (agricultural newborn 600# phosphate), Nongru-700 #, agricultural newborn 2201#, Span-60#, emulsifier T-60, TX-10, agricultural newborn 1601#, agricultural newborn 600#, agricultural newborn 400#; Solvent is selected from xylol, toluene, cyclohexanone, solvent naphtha (trade mark: S-150, S-180, S-200); Stabilizing agent is selected from triphenyl phosphite, epoxychloropropane; Antifreezing agent is selected from ethylene glycol, propane diols, glycerine, urea, inorganic salts such as sodium chloride; Thickener is selected from xanthans, bentonite, aluminium-magnesium silicate; Preservative is selected from benzoic acid, Sodium Benzoate.
With regard to microemulsion, those skilled in the art use following main auxiliary agent, adopt conventional method to prepare: emulsifier is selected from calcium dodecyl benzene sulfonate (agricultural newborn 500#), Nongru-700 #, agricultural newborn 2201#, this dish-60#, Tween-80, TX-10, farming breast 1601, agricultural newborn 600#, agricultural newborn 400#; Cosolvent is selected from methyl alcohol, isopropyl alcohol, n-butanol, ethanol; Solvent is selected from cyclohexanone, N-Methyl pyrrolidone, xylol, toluene, solvent naphtha (trade mark: S-150, S-180, S-200); Stabilizing agent is selected from triphenyl phosphite, epoxychloropropane.
Bactericidal composition of the present invention can provide with the finished product preparation form, and promptly each active substance mixes in the composition; Also can provide, promptly before use in the field, in bucket (jar), directly mix with single agent formulation.Adopt the water dilution to obtain the concentration of required active substance.
The present invention also provides a kind of method of preventing and treating crop pest, before or after crops and/or fruit introduced disease, uses above-mentioned bactericidal composition of the present invention or bactericide to the place of crops and/or fruit and growth or storage.Application process is the conventional application process of agricultural chemicals, for example waters, sprays, spraying, dusting, broadcasts sowing etc.
Compared with prior art, beneficial effect of the present invention is:
(1) compare with single agent, bactericidal composition according to the invention has significant synergistic effect to disease, has improved bactericidal effect;
(2) reduce the medication number of times, reduce drug cost, reduce environmental pollution and residue of pesticide;
(3) composition is made up of the active ingredient of different effects mechanism, and the action site increase helps overcoming and delays the drug-fast generation of germ.
[embodiment]
Come the present invention is further specified below in conjunction with specific embodiment, but do not limit the invention to these embodiments.One skilled in the art would recognize that the present invention contained in claims scope all alternatives, improvement project and the equivalents that possibly comprise.
One, preparation embodiment
Related " % " all is weight percentage in following all preparation proportionings.
Embodiment 1 preparation water dispersible granules
General preparation method is: press formula rate; Former medicine and dispersant, wetting agent, binding agent, filler etc. are mixed, behind comminution by gas stream, obtain pulvis, the water that adds 15-20% mixes; In comminutor, carry out granulation, dry then, screening promptly gets water dispersible granules.
The preparation of the water dispersible granules of the two azoles pyraclostrobins of 50% phenylate:
Take by weighing the two former medicines of azoles of 15% phenylate, the former medicine of 35% pyraclostrobin, 3%TERSPERSE 2700,2% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 3% Nekal BX (nekal), 4%K-12 (lauryl sodium sulfate), 5% microcrystalline cellulose, 3% diatomite, 5% glucose, kaolin and add to 100%; Mix; Make pulvis; Add 20% water, make the water dispersible granules of the two azoles pyraclostrobins of 50% phenylate subsequently according to above-mentioned universal method.
Embodiment 2 preparation suspending agents
General preparation method is: pressing formula rate, is medium with water, former medicine, dispersant, suspending agent and antifreezing agent etc. is joined shear 30min in the high-shear emulsion machine, use the sand mill sand milling again after, obtain suspending agent.
The preparation of the suspending agent of the two azoles pyraclostrobins of 20% phenylate:
Take by weighing the two former medicines of azoles of 13% phenylate, the former medicine of 7% pyraclostrobin, 3%TERSPERSE 4894,2%TERSPERSE 2500,3%TERSPERSE 2425,0.2% xanthans, 1% aluminium-magnesium silicate, 5% ethylene glycol, 0.2% Sodium Benzoate, 0.5% silicone defoaming agent (trade name: s-29 Nanjing "four news" (new ideas applied chemistry article company produces); Deionized water adds to 100%; Process slurry; Carry out sand milling, obtain the suspending agent of the two azoles pyraclostrobins of 20% phenylate.
The preparation of the suspending agent of the two azoles pyraclostrobins of 24% phenylate:
Take by weighing the two former medicines of azoles of 16% phenylate, the former medicine of 8% pyraclostrobin, 2%NNO, 2%TERSPERSE 2500,1% emulsifier T-60,3% Nongru-700 #, 0.15% xanthans, 1% aluminium-magnesium silicate, 5% propane diols, 0.2% Sodium Benzoate, 0.5% silicone defoaming agent; Deionized water adds to 100%; Process slurry; Carry out sand milling, obtain the suspending agent of the two azoles pyraclostrobins of 24% phenylate.
Embodiment 3 preparation wetting powders
General preparation method is: press formula rate, various auxiliary agents, filler, former medicine are mixed after after the Roughpulverizer pulverizing, get into airslide disintegrating mill and fully pulverize the mixed wetting powder that gets.
The preparation of the wetting powder of the two azoles pyraclostrobins of 70% phenylate:
Take by weighing the two former medicines of azoles of 50% phenylate, the former medicine of 20% pyraclostrobin, 5% calcium lignosulfonate, 2% Nekal BX, 1%K-12,5% white carbon, kaolin and add to 100%, mix, obtain the wetting powder of the two azoles pyraclostrobins of 70% phenylate after the pulverizing.
The preparation of the wetting powder of the two azoles pyraclostrobins of 50% phenylate:
Take by weighing the two former medicines of azoles of 40% phenylate, the former medicine of 10% pyraclostrobin, 4% calcium lignosulfonate, 3%TERSPERSE 2700,2% Nekal BX, 2%K-12,5% white carbon, fine particle calcium carbonate and add to 100%; Mix, obtain the wetting powder of the two azoles pyraclostrobins of 50% phenylate after the pulverizing.
Embodiment 4 preparation microemulsions
The preparation of the microemulsion of the two azoles of 15% pyraclostrobin phenylate
Take by weighing the former medicine of 5% pyraclostrobin, the two former medicines of azoles of 10% phenylate, 4%TX-10,5% Nongru-700 #, 4% agricultural newborn 500#, 6% agricultural newborn 1601#, 20% cyclohexanone, 5%N-methyl pyrrolidone, 5% n-butanol, 1% epoxychloropropane; Through dissolving fully and mixing; Deionized water adds to 100%; After stirring, obtain the microemulsion of the two azoles of 15% pyraclostrobin phenylate.
Embodiment 5 preparation aqueous emulsions
The aqueous emulsion of the two azoles of 15% pyraclostrobin phenylate
Take by weighing the two former medicines of azoles of the former medicine of 10% pyraclostrobin, 5% phenylate, 3% agricultural newborn 2201#, 2% NPE (EO=10) phosphate, 3% triphen ethyl-phenol polyoxyethylene ether phosphate (agricultural newborn 600# acid phosphatide), 2%Span-60#, 1% epoxychloropropane; 25% xylol, 10% cyclohexanone, mixed dissolution obtains oil phase.5% ethylene glycol, 0.1% xanthans, 0.2% Sodium Benzoate, deionized water add to 100%, are made into water.The oil adduction is gone into aqueous phase and is fully mixed, and behind high-shear emulsifying, obtains the aqueous emulsion of the two azoles of 15% pyraclostrobin phenylate.
Two, biologically active embodiment
Biologically active embodiment is divided into indoor biologically active embodiment and land for growing field crops biologically active embodiment.
Two azoles of indoor biologically active embodiment 1 phenylate and the composite Toxicity Determination of pyraclostrobin to powdery mildew of cucumber
Subjects powdery mildew of cucumber (Sphaerotheca uliginea) is cultivated by the long-term live body in this research department.
Test method is with reference to " the agricultural industry criteria NY/T 1156.11-2008 of the People's Republic of China (PRC) " spore suspension inoculation method.Select the consistent cucumber seedling of growing way, 24h dries in the shade after spraying is handled.Wash and get the fresh spore that covers with on the powdery mildew cucumber leaves, filter with double gauze, processing spore concentration is the suspension about 100,000/ml, spray inoculation.Postvaccinal examination material natural air drying moves under the thermostatic chamber light in (21-23 ℃) then, and 7-8d backsight blank incidence carries out the classification investigation, calculates preventive effect % with disease index.Through the linear regression analysis between the logarithm value of control efficiency and series concentration, obtain the EC of each medicament
50Value is calculated co-toxicity coefficient (CTC) according to the abundant method of Sun Yun again.
The investigation grade scale:
0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6%～10% of one-piece blade area;
5 grades: lesion area accounts for 11%～20% of one-piece blade area;
7 grades: lesion area accounts for 21%～40% of one-piece blade area;
9 grades: lesion area accounts for more than 40% of one-piece blade area.
The preventive effect computational methods:
The co-toxicity coefficient computational methods: each EC50 that handles that will measure according to Sun Yunpei (Y-P Sun) method is converted into actual poison exponent (ATI); According to the proportioning of mixture, obtain theoretical poison exponent (TTI), calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Toxicity index=(standard medicament EC50/ supplies reagent agent EC50) * 100
Toxicity index (ATI)=[standard medicament EC50/ supplies reagent agent (using with) EC50] * 100 are used in actual measurement with
Theory is used the content (%) of toxicity index * B in using with of content (the %)+B of toxicity index * A in using with of toxicity index (TTI)=A with
Co-toxicity coefficient (CTC)=[actual measurement is used toxicity index (ATI)/theory with and used toxicity index (TTI) with] * 100
If co-toxicity coefficient is greater than 120, showing has synergistic effect; If be starkly lower than 100 (below 80), be indicated as antagonism; Between 100～120, be indicated as adduction.
Two azoles of table 1 phenylate and the composite Toxicity Determination result of pyraclostrobin to powdery mildew of cucumber
Indoor biologically active embodiment 2, the two azoles of phenylate and the composite Toxicity Determination of pyraclostrobin to wheat powdery mildew
Test target wheat powdery mildew (Erysiphe graminis) is cultivated by the long-term live body of this laboratory
Select for use susceptible variety (raising wheat No. 6) potted plant; Treat that seedling grows to 2 leaves～3 leaf phases; Spraying is handled the back and is dried naturally, and the fresh spore of white powder germ that the wheat leaf blade of falling ill was produced in last 24 hour is evenly shaken off to be inoculated on the potted plant wheat seedling of 2～3 leaf phases that chemicals treatment crosses.Postvaccinal examination material moves to 20 ℃, relative moisture 80% left and right sides controllable greenhouse glass room is interior cultivates.Note keeping basin soil moistening every day, investigate according to the classification of blank incidence about 7 days.With reference to the pesticide field efficacy medicine test criterion
(1) bactericidal agent for preventing and treating cereal class powdery mildew.
The investigation grade scale:
0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6%～15% of one-piece blade area;
5 grades: lesion area accounts for 16%～25% of one-piece blade area;
7 grades: lesion area accounts for 26%～50% of one-piece blade area;
9 grades: lesion area accounts for more than 50% of one-piece blade area.
Preventive effect and co-toxicity coefficient computational methods are with indoor biologically active embodiment 1
Two azoles of table 2 phenylate and the composite Toxicity Determination result of pyraclostrobin to wheat powdery mildew
Indoor biologically active embodiment 3, two azoles of phenylate and the composite Toxicity Determination of pyraclostrobin to cucumber anthracnose, potato late blight, the cercospora brown spot of peanut and rice sheath blight disease disease
Subjects: cucumber anthracnose (Colletotrichum lagenarium), potato late blight (Phytophthora infestans), the cercospora brown spot of peanut (Cercospora arachidicola) and rice sheath blight disease (Rhizoctonia solani) are preserved by this laboratory bacterial classification chamber.
Test method: with reference to " the agricultural industry criteria NY/T 1156.2-2006 of the People's Republic of China (PRC) " mycelial growth rate method.Each medicament is established 5 dosage respectively by active constituent content and is handled; Above-mentioned each pathogen is used the PDA medium culture; When treating that bacterium colony has just covered with culture dish, break into the bacterium piece at colony edge, the bacterium piece is moved to the toxic PDA medium central authorities that are mixed with in advance with transfer needle with the card punch of diameter 5mm; Place then in 25 ℃ of incubators and cultivate every processing repetition 4 times.Look CK colony growth situation, adopt the right-angled intersection method to measure and respectively handle colony diameter cm, obtain to proofread and correct and suppress percentage with slide calliper rule.The EC of each medicament is obtained in linear regression analysis between the probit value through inhibiting rate and the logarithm value of series concentration
50Value is calculated co-toxicity coefficient (CTC) according to the abundant method of Sun Yun again.
Drug effect is calculated: two diameters are surveyed in each bacterium colony right-angled intersection, represent the bacterium colony size with its mean.Obtain the colony growth inhibiting rate by following formula: colony growth inhibiting rate %=(the blank bacterium colony increases diameter-chemicals treatment bacterium colony and increases diameter) * 100/ blank bacterium colony increases diameter
The co-toxicity coefficient computational methods are with indoor biologically active embodiment 1
Two azoles of table 3 phenylate and the composite Toxicity Determination result of pyraclostrobin to cucumber anthracnose
Two azoles of table 4 phenylate and the composite Toxicity Determination result of pyraclostrobin to potato late blight
Two azoles of table 5 phenylate and the composite Toxicity Determination result of pyraclostrobin to the cercospora brown spot of peanut
Two azoles of table 6 phenylate and the composite Toxicity Determination result of pyraclostrobin to rice sheath blight disease
Following is land for growing field crops biologically active embodiment 1-4, and wherein employed bactericide is above-mentioned preparation
The prepared preparation of embodiment.
Land for growing field crops biologically active embodiment 1 prevents and treats the regulation of powdery mildew of cucumber field control effectiveness test according to medicine inspecting institute of the Ministry of Agriculture " GB/T 17980.30-2000 pesticide field efficacy medicine test criterion ()---bactericidal agent for preventing and treating powdery mildew of cucumber "; The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 15m
2, 4 repetitions, this test amounts to twice of dispenser.Be in their early stage during dispenser for the first time, execute medicine for the second time after the week.This test is investigated 2 times altogether, and 4 samplings are at random adopted in every experimental plot, investigates 2 strains at every, investigates whole blades of every strain, note
Record disease grade.
The disease grade scale is following:
0 grade: no scab;
1 grade: lesion area accounts for whole below 5% of leaf area;
3 grades: lesion area accounts for 6%～10% of whole leaf area;
5 grades: lesion area accounts for 11%～20% of whole leaf area;
7 grades: lesion area accounts for 21%～40% of whole leaf area;
9 grades: lesion area accounts for whole more than 40% of leaf area.
The drug effect computational methods:
According to investigation result, calculate disease index and preventive effect according to following (1), (2) formula.
In the formula: CK0, CK1 are respectively the forward and backward disease index of blank district dispenser; PT0, PT1 are respectively the forward and backward disease index of chemicals treatment district medicine.
Table 7 is prevented and treated powdery mildew of cucumber field control effectiveness test result
Land for growing field crops biologically active embodiment 2, control pepper anthracnose field control effectiveness test
Carry out field control effectiveness test according to medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion () GB/T17980.33-2000 bactericidal agent for preventing and treating pepper anthracnose ".The experimental field is positioned at the ShaoXing,ZheJiang academy of agricultural sciences.The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 15m
2, 4 repetitions, investigation state of an illness radix before the dispenser, dispenser is 2 times altogether.50 fruits of every sub-district random searching, the percentage classification that accounts for whole fruit area with lesion area.
Grade scale is following:
0 grade: no scab;
1 grade: lesion area accounts for fruit area 2%;
3 grades: lesion area accounts for fruit area 3%～8%;
5 grades: lesion area accounts for fruit area 9%～15%;
7 grades: lesion area accounts for fruit area 16%～25%;
9 grades: lesion area accounts for the fruit area more than 25%.
The drug effect computational methods are with land for growing field crops biologically active embodiment 1
Table 8 control pepper anthracnose field control effectiveness test result
Land for growing field crops biologically active embodiment 3, prevent and treat the rice sheath blight disease field control effectiveness test and carry out according to the regulation of " pesticide field efficacy medicine test criterion () GB/T17980.20-2000---bactericidal agent for preventing and treating rice sheath blight disease "; The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random; Sub-district area 15m2; Repeat for 4 times, investigation state of an illness radix before the dispenser, dispenser is 2 times altogether.Yuecheng District East Lake Zhen Gao Bei Cun experimental field.Diagonal 5 samplings in every experimental plot are investigated 5 clumps that link to each other, totally 25 clumps, are write down total strain number, diseased plant number and sick progression for every.The disease grade scale as
Down:
0 grade: complete stool is anosis;
1 grade: quaterfoil and following each leaf sheath thereof, blade morbidity (is first blade with first sword-like leave);
3 grades: the 3rd blade and following each leaf sheath thereof, blade morbidity;
5 grades: second blade and following each leaf sheath thereof, blade morbidity;
7 grades: sword-like leave blade and following each leaf sheath thereof, blade morbidity;
9 grades: the complete stool morbidity, withered ahead of time.
The drug effect computational methods are with land for growing field crops biologically active embodiment 1
Table 9 is prevented and treated rice sheath blight disease field control effectiveness test result
Land for growing field crops biologically active embodiment 4, prevent and treat the potato late blight field control effectiveness test and carry out according to the regulation of " pesticide field efficacy medicine test criterion () GB/T17980.34-2000---bactericidal agent for preventing and treating potato late blight "; The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 15m
2, 4 repetitions, investigation state of an illness radix before the dispenser, dispenser is 2 times altogether.Village in ages up to ten thousand, Ningbo City's Yinzhou District Qiu mountain pass town experimental field.Every experimental plot diagonal 5 points
Sampling is got 2～3 strains at every, looks into whole blades.
The disease grade scale is following:
0 grade: no scab;
1 grade: lesion area accounts for whole leaf area 5%;
3 grades: lesion area accounts for whole leaf area 6%～10%;
5 grades: lesion area accounts for whole leaf area 11%～20%;
7 grades: lesion area accounts for whole leaf area 21%～50%;
9 grades: lesion area accounts for whole leaf area more than 50%.
The drug effect computational methods are with land for growing field crops biologically active embodiment 1
Table 10 is prevented and treated potato late blight field control effectiveness test result
The result can find out from above-mentioned field control effectiveness test (table 7～10), ability effectively preventing powdery mildew of cucumber, pepper anthracnose, rice sheath blight disease and potato late blight after the two azoles of pyraclostrobin and phenylate are composite
Disease, preventive effect are superior to contrasting medicament.The test scope of medication in to trial crops safety.
In sum, the result of the test of a large amount of indoor biologically active embodiment and land for growing field crops biologically active embodiment shows that the beneficial effect that bactericidal composition according to the invention produces is:
(1) compare with single agent, this bactericidal composition has significant synergistic effect to disease, has improved bactericidal effect;
(2) reduce the medication number of times, lower drug cost, reduce environmental pollution and residue of pesticide;
(3) composition is made up of the active ingredient of different effects mechanism, and the action site increase helps overcoming and delays the drug-fast generation of germ.In field experiment, bactericide of the present invention does not all have obvious harmful effect to trial crops, and leaf look, growing way etc. are all normal, and safety is good, meets the security requirement of pesticidal preparations.
A kind of bactericidal composition that contains two azoles of phenylate and pyraclostrobin of the present invention is described through concrete embodiment.Those skilled in the art can use for reference links such as content appropriate change raw material of the present invention, process conditions and realize corresponding other purpose; Its relevant change does not all break away from content of the present invention; All similar replacements and change will become apparent to those skilled in the art that all to be regarded as and are included within the scope of the present invention.
Claims (9)
1. a bactericidal composition is characterized in that, this bactericidal composition contains following active component A and B:
A, have the two azoles of phenylate of following structural formula;
B, pyraclostrobin;
Wherein the weight ratio of component A and B component is 100: 1～1: 100.
2. bactericidal composition according to claim 1 is characterized in that, the weight ratio of component A and B component is 10: 1～1: 10 in this bactericidal composition.
3. a bactericide is characterized in that, this bactericide contains the described bactericidal composition of each claim of claim 1-2 of 0.1～90 weight %, and surplus is acceptable carrier and an auxiliary agent on the agricultural chemicals.
4. according to the bactericide of claim 3, it is characterized in that this bactericide contains the described bactericidal composition of each claim of claim 1-2 of 3～80 weight %.
5. according to the bactericide of claim 3, it is characterized in that this bactericide contains the described bactericidal composition of each claim of claim 1-2 of 15～70 weight %.
6. according to the described bactericide of each claim of claim 3-6, it is characterized in that this bactericide is mixed with water dispersible granules, suspending agent, wetting powder, microemulsion or aqueous emulsion.
According to the described bactericidal composition of each claim of claim 1-2 in the purposes of preventing and treating powdery mildew, anthracnose, late blight, anthracnose, brown spot and banded sclerotial blight.
8. according to the purposes of claim 7, it is characterized in that said powdery mildew is powdery mildew of cucumber and wheat powdery mildew, anthracnose is cucumber anthracnose, and late blight is a potato late blight, and brown spot is the cercospora brown spot of peanut, and banded sclerotial blight is a rice sheath blight disease.
9. method of preventing and treating crop pest; Before or after crops and/or fruit introduced disease, use described bactericidal composition of each claim of claim 1-2 or the described bactericide of each claim of claim 3-6 to the place of crops and/or fruit and growth or storage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210141468 CN102657199B (en) | 2012-05-08 | 2012-05-08 | Sterilization composition containing difenodiconazole and pyraclostrobin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| CN103125320A (en) * | 2013-02-05 | 2013-06-05 | 中国科学院东北地理与农业生态研究所 | Prevention method of beetroot brown blotch |
| WO2014082881A1 (en) | 2012-11-27 | 2014-06-05 | Basf Se | Substituted 2-[phenoxy-phenyl]-1-[1,2,4]triazol-1-yl-ethanol compounds and their use as fungicides |
| WO2014082871A1 (en) | 2012-11-27 | 2014-06-05 | Basf Se | Substituted 2-[phenoxy-phenyl]-1-[1,2,4]triazol-1-yl-ethanol compounds and their use as fungicides |
| EP2783569A1 (en) * | 2013-03-28 | 2014-10-01 | Basf Se | Compositions comprising a triazole compound |
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| CN102177902A (en) * | 2011-03-19 | 2011-09-14 | 陕西汤普森生物科技有限公司 | Synergetic sterilization composition containing pyraclostrobin |
| CN102228043A (en) * | 2011-05-06 | 2011-11-02 | 浙江泰达作物科技有限公司 | Bactericide water dispersible granules compounded from difenoconazole and pyraclostrobin and preparation method thereof |
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| WO2012045737A1 (en) * | 2010-10-07 | 2012-04-12 | Basf Se | Use of strobilurins for increasing the gluten strength in winter cereals |
| CN102177902A (en) * | 2011-03-19 | 2011-09-14 | 陕西汤普森生物科技有限公司 | Synergetic sterilization composition containing pyraclostrobin |
| CN102228043A (en) * | 2011-05-06 | 2011-11-02 | 浙江泰达作物科技有限公司 | Bactericide water dispersible granules compounded from difenoconazole and pyraclostrobin and preparation method thereof |
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| WO2014082881A1 (en) | 2012-11-27 | 2014-06-05 | Basf Se | Substituted 2-[phenoxy-phenyl]-1-[1,2,4]triazol-1-yl-ethanol compounds and their use as fungicides |
| WO2014082871A1 (en) | 2012-11-27 | 2014-06-05 | Basf Se | Substituted 2-[phenoxy-phenyl]-1-[1,2,4]triazol-1-yl-ethanol compounds and their use as fungicides |
| CN103125320A (en) * | 2013-02-05 | 2013-06-05 | 中国科学院东北地理与农业生态研究所 | Prevention method of beetroot brown blotch |
| EP2783569A1 (en) * | 2013-03-28 | 2014-10-01 | Basf Se | Compositions comprising a triazole compound |
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| CN102657199B (en) | 2013-08-07 |
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Address after: 311106 No. 172, Zhangjiadun Road, Tangqi Town, Linping District, Hangzhou, Zhejiang Patentee after: Zhejiang Yulong Pharmaceutical Co.,Ltd. Address before: No. 172, zhangjiadun Road, Tangqi Town, Yuhang District, Hangzhou City, Zhejiang Province, 311106 Patentee before: Hangzhou Udragon Chemical Co.,Ltd. |