CN102657116B - Method for realizing hydrostatic tests of aquatic organisms based on principle of communicating vessels - Google Patents
Method for realizing hydrostatic tests of aquatic organisms based on principle of communicating vessels Download PDFInfo
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- CN102657116B CN102657116B CN201210127270XA CN201210127270A CN102657116B CN 102657116 B CN102657116 B CN 102657116B CN 201210127270X A CN201210127270X A CN 201210127270XA CN 201210127270 A CN201210127270 A CN 201210127270A CN 102657116 B CN102657116 B CN 102657116B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention provides a device for realizing hydrostatic tests of aquatic organisms based on the principle of communicating vessels and a method for the device. The device comprises a water inlet, a water inlet tube, a water inlet valve, a water inlet tube filter sieve, a piezometer, a pressure-resistant carrier box, an observation window, a water outlet filter sieve, a water outlet, a water outlet tube and series water outlet holes, wherein both sides of the pressure-resistant carrier box are provided with the water inlet tube and the water outlet tube respectively; the observation window is formed on the pressure-resistant carrier box and is provided with the piezometer, and the bottom part of the pressure-resistant carrier box is provided with the water outlet; and the water outlet tube is provided with a row of series water outlet holes, and the inner part, close to the pressure-resistant carrier box, of the water inlet tube is provided with the water inlet valve. The device has a simple structure and can realize hydrostatic tests of short period or long period aquatic organisms under various pressures.
Description
Technical field
The invention belongs to aquatic science experiment field, specially refer to the hydrostatic test device and method.
Background technology
Pressure is one of physical agent very important in the water environment, and size is according to Pascal's formula p=ρ gh gained, and habituation is also referred to as hydraulic pressure.Pressure is with the relation between the vertiginous characteristics of the depth of water and gas volume and the pressure in the water, plays an important role in the process of the evolution of the transformation of fish proportion regulation mechanism and air bladder.
At present, realize that both at home and abroad the several method of pressure is analyzed as follows: Gui Jianfang (1990) experiment belongs to the molecule experiment at fish-egg, and the hydrostatic pressure device adopts industrial booster, has utilized the good pressure transmission of water, carries out in confined space; Liao Xiaodong (1995) does not adopt pressurizer only to rely on the water body height change under nature, simulates variation in water pressure in laboratory containers, pressure range receptor effect of altitude, and difference in height has only realized the cms magnitude; Kim(2011) experimental technique has been realized the automatic sink-float of net cage, and purpose of design is to hide wave, guarantees net cage safety; Above method has practicality in field separately.But, current international water power at home utilizes the aspect, because the river pressure that effect causes of building a dam changes hydrobiological influence, the influence (especially demersal fishes) that various fish is distributed as the hydraulic pressure that increases, the change of fish behavior under the influence that the fish glue fish are arranged, heavy property fish-egg (the comprising the mandarin sturgeon ovum) nature problems such as hatching, then can't realize that reason is as follows by above-mentioned instrument:
The hydrostatic pressure device is aspect structure, and water body is strict airtight, and for living body biological, the bait that organism is required and oxygen can't enter; And water body can not obtain exchange, and water body is rotten, is unfavorable for live body is launched long-term experiment, therefore is not suitable for experiment made on the living; In addition, confined space is limited, and is inapplicable for the large-scale fish of most adults.
Do not adopt pressurizer under the nature, laboratory containers is aspect the change of water body pressure, and the numerical value interval is narrow and small, influences experiment effect.
Rely on and realize in the water body that there is following shortcoming in the pressure change:
1, the biological behavior of experiment changes difficult the observation and record under water body;
2, at the deep water body that has than high current speed, net cage is wayward.
Summary of the invention:
The object of the present invention is to provide a kind of device that uses law of connected vessels to realize the aquatile hydrostatic test, and utilize this device to carry out aquatile hydrostatic test method, this invention can realize building a dam effect on land to the hydrobiological influence experiment of live body, be convenient to bait throwing in and keep water quality good, for long-term experiment can be beneficial to the bigger pressure of realization and change interval for possibility.
For achieving the above object, technical scheme of the present invention is:
The present invention includes: water inlet, water inlet pipe, inlet valve, water inlet pipe screen pack, pressure gage, resistance to compression carrier case, perspective window, outlet pipe screen pack, discharge outlet, outlet pipe, serial apopore, wherein the dual-side of resistance to compression carrier case is equipped with water inlet pipe and outlet pipe, have the perspective window on the resistance to compression carrier case, on the perspective window pressure gage is housed, resistance to compression carrier case bottom has discharge outlet, have the serial apopore of a row on the outlet pipe, water inlet pipe is equipped with inlet valve near the inside of resistance to compression carrier case.
Water inlet pipe is equipped with the water inlet pipe screen pack with the position that resistance to compression carrier case contacts.
Outlet pipe is equipped with the outlet pipe screen pack with the position that resistance to compression carrier case contacts.
Utilize this device to carry out aquatile hydrostatic test method, the steps include:
One, preparation before the experiment: water inlet, delivery port, valve are in closed condition, and carry out the data acquisition that biology is measured, biochemical sample is sampled to testing biology;
Two, experimentize: open inlet valve, inject greater than closing inlet valve after the organism depth of water highly from water inlet, open the perspective window on the resistance to compression carrier case, it is biological to put into experiment, shut the perspective window, open 11i in the serial delivery port of testing desired height h correspondence, the following serial delivery port of this height h is Close All then, calculate pressure P by P=ρ gh, open inlet valve, be filled to experiment desired height h, height is at series delivery port 11 places, outlet pipe place mark, from pressure gage, read actual pressure at the bottom of the resistance to compression carrier case, adjust the water inlet water intake velocity, make it to go out water speed less than serial delivery port maximum;
Three experimental data collections: bait enters resistance to compression carrier case by water inlet with current, by the adaptive behavior of perspective window observation experiment biology to pressure, after experiment finishes, close inlet valve, open the little discharge outlet of serial delivery port lowest order, when water level reaches this discharge outlet height, regulate again the casing depth of water to organism highly quite after, it is biological to take out experiment, image data.
The clean system of cleaning politics, economics, organization, and ideology: water inlet stops into water, opens inlet valve and discharge outlet, and with the water emptying, cleaning resistance to compression carrier case is closed water inlet, serial delivery port, inlet valve then, uses in order to experiment next time.
Good effect of the present invention is:
1, use law of connected vessels to realize that on land water body pressure changes, the effect that realizes building a dam is to the hydrobiological influence experiment of live body.
2, it is good to use law of connected vessels to realize that on land the change of water body pressure is conducive in bait throwing in and maintenance water quality, for long-term experiment made on the living realizes providing adequate condition.
3, use law of connected vessels to realize that on land water body pressure changes, it is interval to be conducive to realize that bigger pressure changes.
4, use law of connected vessels to realize that on land water body pressure changes, be conducive to ethological observation.
Description of drawings
Fig. 1 is structural representation of the present invention.
Embodiment
Further specify the specific embodiment of the present invention below in conjunction with drawings and Examples
Referring to Fig. 1: use law of connected vessels to realize that the device of aquatile hydrostatic test comprises water inlet 1, water inlet pipe 2, inlet valve 3, water inlet pipe screen pack 4, pressure gage 5, resistance to compression carrier case 6, perspective window 7, outlet pipe screen pack 8, discharge outlet 9, outlet pipe 10, serial apopore 11, wherein the dual-side of resistance to compression carrier case 6 is equipped with water inlet pipe 2 and outlet pipe 10, and communicates; Have perspective window 7 on the resistance to compression carrier case 6, on the perspective window 7 pressure gage 5 is housed, resistance to compression carrier case 6 bottoms have discharge outlet 9, have the serial apopore 11 of a row on the outlet pipe 10; Near the inside, position of resistance to compression carrier case 6 inlet valve 3 is housed at water inlet pipe 2.
Utilize device shown in Figure 1, carry out the method for aquatile hydrostatic test, specific implementation method is as follows:
One 7 monthly age of example mandarin sturgeon 65m and 175m depth of water pressure intensity testing
Preparation before one experiment
1, water inlet 1, discharge outlet 9, inlet valve 3 are in closed condition;
2, to the mandarin sturgeon preceding data acquisition that experimentizes: total length (TL), body long (BL), fork long (FL), height (BH), body weight (BW) and tail vein blood 3 each 2ml of pipe.
Two water fillings, put into mandarin sturgeon and pressure and regulate
1 opens water inlet 1, opens inlet valve 3, water filling in the resistance to compression carrier case, depth of water BH+10cm.
2 valve-offs 3 are opened 7 perspective windows and put into mandarin sturgeon in resistance to compression carrier casees, shut 7 perspective windows.
3 open the delivery port 1165 at 65m place, and calculating gained bottom half pressure by P=ρ gh is P=1000 * 9.8 * 65=6.37 * 105 handkerchiefs.
4 open valve 3, are filled to 65m, and from pressure gage 5 pin-point readings, this value is resistance to compression
The accurate pressure values in 6 ends of carrier case.
5 adjust water inlet 1 water intake velocity, make it to go out water speed less than serial delivery port 11 maximums, guarantee stable the stable of casing pressure that reach of linker height.
Three experimental data collections
1 bait enters resistance to compression carrier case 6 by water inlet 1 with current, observes mandarin sturgeon feeding behaviour in casing.
2 observe mandarin sturgeons in the injecting process and during 65m stress behavior and adaptive behavior can be by perspective window 71,72,73 ... 7i observes.
4 experiments are after 20 days, and valve-off 3 opens 111.And then open valve 3, make the water body height be down to 111 delivery port height.
5 regulate the casing depth of water to BH+10cm again by valve 9, take out mandarin sturgeon, the back image data is handled in experiment: total length (TL1), body long (BL1), fork long (FL1), height (BH1), body weight (BW1) and tail vein blood 3 each 2ml of pipe, lethality, survival rate, fish glue functional check etc.
Four adjustment pressure are the 175m depth of water and relevant data
Experimental session pressure is adjusted
Water inlet 1 stops into water and closes inlet valve 3 again, then closes 65m place delivery port 1165, opens 175m place delivery port 11175, and calculating gained bottom half pressure by P=ρ gh is P=1000 * 9.8 * 175=17.15 * 105 handkerchiefs.
Repeat the operation in the 3rd step, finish until experiment.
Five cleaning systems:
Water inlet 1 stops into water, opens inlet valve 3, opens discharge outlet 9 again, and water is discharged through discharge outlet 9 in the water inlet pipe.
Cleaning resistance to compression carrier case 6 is removed excreta, and desilting etc. are discharged through discharge outlet 9.
Close water inlet 1, inlet valve 3, discharge outlet 9 then, next time, experiment was stand-by.
Example two .5 mullet in age 40m and 200m depth of water pressure intensity testing.
Preparation before one experiment
1, water inlet 1, discharge outlet 9, inlet valve 3 are in closed condition;
2 pairs of mullets preceding data acquisition that experimentizes: total length (TL), body long (BL), fork long (FL), height (BH), body weight (BW) and tail vein blood 3 each 2ml of pipe etc.
Two water fillings, put into mullet and pressure and regulate
1 opens water inlet 1, and inlet valve 3 is to the 6 interior water fillings of resistance to compression carrier case, depth of water BH+10cm.
2 close inlet valve 3, open and shut after perspective window 7 is put into mullet.
3 open the delivery port 1140 at 40m place, and calculating gained bottom half pressure by P=ρ gh is P=1000 * 9.8 * 40=3.92 * 105 handkerchiefs.
4 open valve 3, are filled to 40m.From pressure gage 5 pin-point readings, this value is the accurate pressure values in 6 ends of resistance to compression carrier case.
5 adjust water inlet 1 water intake velocity, make it to go out water speed less than serial delivery port 11 maximums, guarantee stable the stable of casing pressure that reach of linker height.
Three experimental data collections
1 bait enters resistance to compression carrier case 6 by water inlet with current, observes the mullet situation of ingesting in casing.
2 observe mullets in the injecting process and during 40m stress behavior and adaptive behavior can pass through 7 series 71,72,73 ... 7i perspective window is observed.
4 experiments were closed inlet valve 3 after 20 days, opened serial delivery port 111, and then opened inlet valve 3, made the water body height be down to the height of serial delivery port 111.
5 regulate the casing depth of water to BH+10cm again by discharge outlet 9, take out mullet, the back image data is handled in experiment: total length (TL1), body long (BL1), fork long (FL1), height (BH1), body weight (BW1) and tail vein blood 3 each 2ml of pipe, lethality, survival rate, fish glue functional check etc.
Four adjustment pressure are the 200m depth of water and relevant data
1 experimental session pressure is adjusted
Water inlet 1 stops into water and closes inlet valve 3 again, then closes the 40m place and goes out serial delivery port 1140, opens the serial delivery port 11200 in 200m place, and calculating gained bottom half pressure by P=ρ gh is P=1000 * 9.8 * 200=19.6 * 105 handkerchiefs.
2 repeated for the 3rd step, finished until experiment.
Five cleaning systems:
Water inlet 1 stops into water, opens inlet valve 3, opens discharge outlet 9 again, and water is discharged through discharge outlet 9 in the water inlet pipe.
Cleaning resistance to compression carrier case 6 is removed excreta, and desilting etc. are discharged through discharge outlet 9.
Close water inlet 1, inlet valve 3, discharge outlet 9 then, next time, experiment was stand-by.
Example acipenser dabryanus 80m in 34 age and 140m depth of water pressure intensity testing
Preparation before one experiment
1 water inlet, delivery port, valve are in closed condition.
2 pairs of acipenser dabryanus preceding data acquisition that experimentizes: total length (TL), body long (BL), fork long (FL), height (BH), body weight (BW) and tail vein blood 3 each 2ml of pipe etc.
Two water fillings, put into acipenser dabryanus and pressure and regulate
1 opens water inlet 1 and inlet valve 3, to the 6 interior water fillings of resistance to compression carrier case, depth of water BH+10cm.
2 close inlet valve 3, open and shut after perspective window 7 is put into acipenser dabryanus.
3 open the serial delivery port 1180 at 80m place, and calculating gained bottom half pressure by P=ρ gh is P=1000 * 9.8 * 80=7.84 * 105 handkerchiefs.
4 open inlet valve 3, are filled to 80m, and from pressure gage 5 pin-point readings, this value is the accurate pressure values in 6 ends of resistance to compression carrier case.
5 adjust water inlet 1 water intake velocity, make it to go out water speed less than serial delivery port 11 maximums, guarantee stable the stable of casing pressure that reach of linker height.
Three experimental data collections
1 bait enters resistance to compression carrier case by water inlet 1 with current, observes the acipenser dabryanus situation of ingesting in casing.
2 observe acipenser dabryanus in the injecting process and during 80m stress behavior and adaptive behavior can pass through 7 series 71,72,73 ... 7i perspective window is observed.
4 experiments are after 20 days, and valve-off 3 opens 111.And then open valve 3, make the water body height be down to 111 delivery port height.
5 regulate the casing depth of water to BH+10cm again by valve 9, take out acipenser dabryanus, the back image data is handled in experiment: total length (TL1), body long (BL1), fork long (FL1), height (BH1), body weight (BW1) and tail vein blood 3 each 2ml of pipe, lethality, survival rate, fish glue functional check etc.
Four adjustment pressure are the 140m depth of water and relevant data
1 experimental session pressure is adjusted
Water inlet 1 stops into water valve-off 3 again, then closes 80m place delivery port 1180, opens 140m place delivery port 11140 in corresponding height position downwards, is P=1000 * 9.8 * 140=13.72 * 105 handkerchiefs by P=ρ gh calculating gained bottom half pressure.
2 repeated for the 3rd step, finished until experiment.
Five cleaning systems
Water inlet 1 stops into water, opens inlet valve 3, opens discharge outlet 9 again, and water is discharged through discharge outlet 9 in the water inlet pipe.
Cleaning resistance to compression carrier case 6 is removed excreta, and desilting etc. are discharged through discharge outlet 9.
Close water inlet 1, inlet valve 3, discharge outlet 9 then, next time, experiment was stand-by.
Experimental rig of the present invention and method can realize short-term or simulate the water pressure test of aquatile under the pressure that natural dam forms for a long time that because length is limited, embodiment enumerates no longer one by one, all belongs to the scope of protection of present invention.
Claims (1)
1. use law of connected vessels to realize the method for aquatile hydrostatic test, it is characterized in that:
The step of this method is as follows:
One, preparation before the experiment: water inlet, delivery port, valve are in closed condition, and carry out the data acquisition that biology is measured, biochemical sample is sampled to testing biology;
Two, experimentize: open inlet valve (3), inject greater than closing inlet valve (3) after the organism depth of water highly from water inlet (1), open the perspective window (7) on the resistance to compression carrier case (6), it is biological to put into experiment, opens in the serial delivery port (11) of experiment desired height h correspondence 11
iThe following serial delivery port (11) of this height h is Close All then, calculate pressure P by P=ρ gh, open inlet valve (3), be filled to experiment desired height h, height is located mark at outlet pipe place series delivery port (11), reads actual pressure at the bottom of the resistance to compression carrier case from pressure gage (5), adjust water inlet (1) water intake velocity, make it to go out water speed less than serial delivery port (11) maximum;
Three experimental data collections: bait enters resistance to compression carrier case (6) by water inlet (1) with current, by the adaptive behavior of perspective window (7) observation experiment biology to pressure, after experiment finishes, close inlet valve (3), open the little discharge outlet of serial delivery port (11) lowest order, when water level reaches this discharge outlet height, regulate again the casing depth of water to organism highly quite after, it is biological to take out experiment, image data;
The clean system of cleaning politics, economics, organization, and ideology: water inlet (1) stops into water, open inlet valve (3) and discharge outlet (9), with the water emptying, cleaning resistance to compression carrier case (6), close water inlet (1), serial delivery port (11), inlet valve (3) then, use in order to experiment next time.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210127270XA CN102657116B (en) | 2012-04-27 | 2012-04-27 | Method for realizing hydrostatic tests of aquatic organisms based on principle of communicating vessels |
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| CN201210127270XA CN102657116B (en) | 2012-04-27 | 2012-04-27 | Method for realizing hydrostatic tests of aquatic organisms based on principle of communicating vessels |
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| CN102657116B true CN102657116B (en) | 2013-08-21 |
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Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0315332A (en) * | 1989-06-13 | 1991-01-23 | Nkk Corp | High pressure water tank for rearing organism |
| JPH03151818A (en) * | 1989-11-10 | 1991-06-28 | Kaiyo Kagaku Gijutsu Center | Experimental facility for deep-sea microorganism |
| JP2735922B2 (en) * | 1990-01-31 | 1998-04-02 | 海洋科学技術センター | High-pressure experimental tank |
| DE102006041063A1 (en) * | 2006-09-01 | 2008-03-13 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | High pressure device i.e. continental deep sea aquarium, has shaft that is extended into earth`s crust, and pressure device arranged in shaft, where device has inner pressure chamber that is pressure-resistantato exterior overpressure |
| CN201557452U (en) * | 2009-09-25 | 2010-08-25 | 湛江国联水产开发股份有限公司 | Shrimp culturing high-elevation pond |
| CN201821761U (en) * | 2010-04-09 | 2011-05-11 | 水利部交通运输部国家能源局南京水利科学研究院 | Tester for gas bubble disease |
| CN201860647U (en) * | 2010-10-18 | 2011-06-15 | 嘉兴学院 | Fish tank capable of automatically changing water and feeding |
| CN102297865B (en) * | 2011-05-27 | 2013-02-20 | 宁波大学 | Biological water quality monitoring system by fish behaviors and monitoring method thereof |
| CN202618019U (en) * | 2012-04-27 | 2012-12-26 | 中国水产科学研究院长江水产研究所 | Device for realizing aquatic organism water pressure experiment by using principle of communicating vessels |
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