CN102657116A - Device for realizing hydrostatic tests of aquatic organisms based on principle of communicating vessels and method for device - Google Patents

Device for realizing hydrostatic tests of aquatic organisms based on principle of communicating vessels and method for device Download PDF

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Publication number
CN102657116A
CN102657116A CN201210127270XA CN201210127270A CN102657116A CN 102657116 A CN102657116 A CN 102657116A CN 201210127270X A CN201210127270X A CN 201210127270XA CN 201210127270 A CN201210127270 A CN 201210127270A CN 102657116 A CN102657116 A CN 102657116A
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water
water inlet
resistance
pressure
carrier case
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CN102657116B (en
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李伟
危起伟
杜浩
张辉
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Huazhong Agricultural University
Yangtze River Fisheries Research Institute CAFS
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Huazhong Agricultural University
Yangtze River Fisheries Research Institute CAFS
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The invention provides a device for realizing hydrostatic tests of aquatic organisms based on the principle of communicating vessels and a method for the device. The device comprises a water inlet, a water inlet tube, a water inlet valve, a water inlet tube filter sieve, a piezometer, a pressure-resistant carrier box, an observation window, a water outlet filter sieve, a water outlet, a water outlet tube and series water outlet holes, wherein both sides of the pressure-resistant carrier box are provided with the water inlet tube and the water outlet tube respectively; the observation window is formed on the pressure-resistant carrier box and is provided with the piezometer, and the bottom part of the pressure-resistant carrier box is provided with the water outlet; and the water outlet tube is provided with a row of series water outlet holes, and the inner part, close to the pressure-resistant carrier box, of the water inlet tube is provided with the water inlet valve. The device has a simple structure and can realize hydrostatic tests of all aquatic organisms under various pressures.

Description

The utilization law of connected vessels is realized the device and method of aquatile hydrostatic test
Technical field
The invention belongs to aquatic science experimental provision field, specially refer to the hydrostatic test device.
Background technology
Pressure is one of physical agent very important in the water environment, and size is according to Pascal's formula p=ρ gh gained, and habituation is also referred to as hydraulic pressure.Pressure is with the relation between the vertiginous characteristics of the depth of water and gas volume and the pressure in the water, in the process of the evolution of the transformation of fish proportion regulation mechanism and air bladder, plays an important role.
At present, realize that both at home and abroad the several method analysis of pressure is following: Gui Jianfang (1990) experiment belongs to the molecule experiment to fish-egg, and the hydrostatic pressure device adopts industrial booster, has utilized the good pressure transmission property of water, in confined space, carries out; Liao Xiaodong (1995) does not adopt pressurizer only to rely on the water body height change under nature, in laboratory containers, simulates variation in water pressure, pressure receptor effect of altitude, and difference in height has only realized the cms magnitude; The experimental technique of Kim (2011) has been realized the automatic sink-float of net cage, and purpose of design is to hide wave, guarantees net cage safety; Above method has practicality in field separately.But; Current international at home water power utilizes the aspect; Because of the river pressure that effect causes of building a dam changes hydrobiological influence, the influence (especially demersal fishes) that various fish is distributed like the hydraulic pressure that increases, the change of fish behavior under the influence that the fish glue fish are arranged, heavy property fish-egg (the comprising the mandarin sturgeon ovum) nature problems such as hatching, then can't realize through above-mentioned instrument; Reason is following: the hydrostatic pressure device is aspect structure; Water body is strict airtight, and for living body biological, bait that organism is required and oxygen can't get into; And water body can not obtain exchange, and water body is rotten, is unfavorable for live body is launched long-term experiment, therefore is not suitable for experiment made on the living; In addition, confined space is limited, and is inapplicable for the large-scale fish of most adults.
Do not adopt pressurizer under the nature, laboratory containers is aspect the change of water body pressure, and the numerical value interval is narrow and small, influences experiment effect.
Rely on and realize in the water body that there is following shortcoming in the pressure change:
A tests biological behavior and changes difficult the observation and record under water body
B is at the deep water body that has than high current speed, and net cage is wayward.
Summary of the invention:
The object of the present invention is to provide a kind of device that uses law of connected vessels to realize the aquatile hydrostatic test; And utilize this device to carry out aquatile hydrostatic test method; This invention can realize building a dam effect to the hydrobiological influence experiment of live body in the land; Be convenient to bait throwing in and keep water quality good,, be beneficial to the bigger pressure of realization and change interval for long-term experiment can supply possibility.
For realizing above-mentioned purpose, technical scheme of the present invention is:
The present invention includes: water inlet, water inlet pipe, inlet valve, water inlet pipe screen pack, pressure gage, resistance to compression carrier case, perspective window, outlet pipe screen pack, discharge outlet, outlet pipe, serial apopore; Wherein the dual-side of resistance to compression carrier case is equipped with water inlet pipe and outlet pipe; Have the perspective window on the resistance to compression carrier case, on the perspective window pressure gage is housed, resistance to compression carrier case bottom has discharge outlet; Have the serial apopore of a row on the outlet pipe, water inlet pipe is equipped with inlet valve near the inside of resistance to compression carrier case.
Water inlet pipe is equipped with the water inlet pipe screen pack with the position that resistance to compression carrier case contacts.
Outlet pipe is equipped with the outlet pipe screen pack with the position that resistance to compression carrier case contacts.
Utilize this device to carry out aquatile hydrostatic test method, the steps include:
One, preparation before the experiment: water inlet, delivery port, valve are in closed condition, and carry out the data acquisition that biology is measured, biochemical sample is sampled to testing biology;
Two, experimentize: open inlet valve; After water inlet injects the depth of water greater than the organism height, close inlet valve, open the perspective window on the resistance to compression carrier case, put into the experiment biology; Shut the perspective window, open in the corresponding serial delivery port of experiment desired height h 11 i, serial delivery port below this height h is Close All then, calculates pressure P by P=ρ gh; Open inlet valve; Be filled to experiment desired height h, height is series delivery port 11 places mark at the outlet pipe place, from pressure gage, reads actual pressure at the bottom of the resistance to compression carrier case; Adjustment water inlet water intake velocity makes it to go out water speed less than serial delivery port maximum;
Three experimental data collections: bait gets into resistance to compression carrier case by water inlet with current, through the adaptive behavior of perspective window observation experiment biology to pressure, after experiment finishes; Close inlet valve; Open the little discharge outlet of serial delivery port lowest order, when water level reaches this discharge outlet height, regulate once more the casing depth of water to organism highly quite after; It is biological to take out experiment, image data.
The clean system of cleaning politics, economics, organization, and ideology: water inlet stops into water, opens inlet valve and discharge outlet, and with the water emptying, cleaning resistance to compression carrier case is closed water inlet, serial delivery port, inlet valve then, uses in order to experiment next time.
Good effect of the present invention is:
1, the utilization law of connected vessels realizes that on land water body pressure changes, and the effect that realizes building a dam is to the hydrobiological influence experiment of live body.
2, the change of realization water body pressure helps in bait throwing in and keeps water quality good the utilization law of connected vessels on land, for long-term experiment made on the living realizes providing adequate condition.
3, the utilization law of connected vessels realizes that on land water body pressure changes, and it is interval to help realizing that bigger pressure changes.
4, the utilization law of connected vessels realizes that on land water body pressure changes, and helps ethological observation.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Embodiment
Further specify embodiment of the present invention below in conjunction with accompanying drawing and embodiment
Referring to Fig. 1: the utilization law of connected vessels realizes that the device of aquatile hydrostatic test comprises water inlet 1, water inlet pipe 2, inlet valve 3, water inlet pipe screen pack 4, pressure gage 5, resistance to compression carrier case 6, perspective window 7, outlet pipe screen pack 8, discharge outlet 9, outlet pipe 10, serial apopore 11; Wherein the dual-side of resistance to compression carrier case 6 is equipped with water inlet pipe 2 and outlet pipe 10, and communicates; Have perspective window 7 on the resistance to compression carrier case 6, on the perspective window 7 pressure gage 5 is housed, resistance to compression carrier case 6 bottoms have discharge outlet 9, have the serial apopore 11 of a row on the outlet pipe 10; Near the inside, position of resistance to compression carrier case 6 inlet valve 3 is housed at water inlet pipe 2.
Water inlet pipe 2 is connected the position that contacts water inlet pipe screen pack 4 is housed with resistance to compression carrier case 6.
Outlet pipe 10 is connected the position that contacts outlet pipe screen pack 8 is housed with resistance to compression carrier case 6.Water inlet pipe 2 and outlet pipe 10, for compression-resistant material is processed, diameter 0.3 ~ 1m, sealing is good.Current and bait flow into water inlet pipe 2 through water inlet 1, guaranteed in the resistance to compression carrier case 6 that the experiment biology can be ingested and in long term survival and the casing water quality good.Open and close the water level of controlling water inlet pipe 2 through inlet valve 3.Water inlet pipe screen pack 4 is processed for the iron wire material with outlet pipe screen pack 8, is installed in the junction of water inlet pipe 2 and outlet pipe 10 and resistance to compression carrier case 6 respectively, is to test biological water inlet pipe 2 and the outlet pipe 10 of getting into for preventing respectively, guarantees the accuracy of testing.The mesh designing requirement of screen pack is less than fish body width with greater than the bait diameter, and assurance experiment biology can not get into water inlet pipe and blow water pipe while bait and can pass through mesh.
Pressure gage 5 is connected on the perspective window 7, the convenient pressure of pressure gage position of accurately reading.Resistance to compression carrier case 6 usefulness compression-resistant materials are processed, and sealing is good.Perspective window 7 possesses out and closes function, is used for the observation experiment material to the reaction of hydraulic pressure and putting into of experiment material, can all be provided with all around.Discharge outlet 9 is positioned at bottom half, is used for the casing draining, the effect of leaking water when playing clean box body.Series apopore 11 links to each other with outlet pipe and communicates, and with outlet pipe junction good seal.Be provided with at a distance from 1m or 2m vertical height along outlet pipe is every, indicate height, be numbered 11 from low to high 1, 11 2, 11 311 i, play and regulate depth of water effect, regulate the pressure in the resistance to compression carrier case with this, other has drainage.Series apopore 11 is installed on the outlet pipe outside, leans on the casing far-end.Whenever at a distance from 1 ~ 2m an apopore is installed from outlet pipe and casing junction, until delivery port.Apopore diameter 12 ~ 15cm is connected in outlet pipe, is opened on the outlet pipe both sides, and the outlet pipe current are discharged through apopore, and when operation experiment water inlet discharge less than the apopore water yield.Bait gets into casing from water inlet with water inlet pipe under current and action of gravity.
Utilize device shown in Figure 1, carry out the method for aquatile hydrostatic test, the practical implementation method is following:
One 7 monthly age of instance mandarin sturgeon 65m and 175m depth of water pressure intensity testing
Preparation before one experiment
1, water inlet 1, discharge outlet 9, inlet valve 3 are in closed condition;
2, to the mandarin sturgeon preceding data acquisition that experimentizes: total length (TL), body long (BL), fork long (FL), height (BH), body weight (BW) and tail vein blood 3 each 2ml of pipe.
Two water fillings, put into mandarin sturgeon and pressure and regulate
1 opens water inlet 1, opens inlet valve 3, water filling in resistance to compression carrier case, depth of water BH+10cm.
2 valve-offs 3 are opened 7 perspective windows and in resistance to compression carrier case, are put into mandarin sturgeon, shut 7 perspective windows.
3 open the delivery port 11 at 65m place 65, calculating gained bottom half pressure by P=ρ gh is P=1000 * 9.8 * 65=6.37 * 10 5Handkerchief.
4 open valve 3, are filled to 65m, and from pressure gage 5 pin-point readings, this value is resistance to compression
The accurate pressure values in 6 ends of carrier case.
5 adjustment water inlets, 1 water intake velocity makes it to go out water speed less than serial delivery port 11 maximums, guarantees stable the stable of casing pressure that reach of linker height.
Three experimental data collections
1 bait gets into resistance to compression carrier case 6 by water inlet 1 with current, observes mandarin sturgeon feeding behaviour in casing.
2 observe mandarin sturgeons in the injecting process and during 65m stress behavior can be with adaptive behavior through having an X-rayed window 7 1, 7 2, 7 37 iObserve.
4 experiments are after 20 days, and valve-off 3 opens 11 1And then open valve 3, make the water body height reduce to 11 1The delivery port height.
5 regulate the casing depth of water to BH+10cm once more by valve 9, take out mandarin sturgeon, and the back image data is handled in experiment: total length (TL 1), the long (BL of body 1), the long (FL of fork 1), height (BH 1), body weight (BW 1) and tail vein blood 3 each 2ml of pipe, lethality, survival rate, fish glue functional check etc.
Four adjustment pressure are the 175m depth of water and relevant data
The adjustment of experimental session pressure
Water inlet 1 stops into water and closes inlet valve 3 again, then closes 65m place delivery port 11 65, open 175m place delivery port 11 175, calculating gained bottom half pressure by P=ρ gh is P=1000 * 9.8 * 175=17.15 * 10 5Handkerchief.
Repeat the operation in the 3rd step, finish until experiment.
Five cleaning systems:
Water inlet 1 stops into water, opens inlet valve 3, opens discharge outlet 9 again, and water is discharged through discharge outlet 9 in the water inlet pipe.
Cleaning resistance to compression carrier case 6 is removed excreta, and desilting etc. are discharged through discharge outlet 9.
Close water inlet 1, inlet valve 3, discharge outlet 9 then, next time, experiment was for use.
  
Instance two .5 mullet in age 40m and 200m depth of water pressure intensity testing.
Preparation before one experiment
1, water inlet 1, discharge outlet 9, inlet valve 3 are in closed condition;
2 pairs of mullets preceding data acquisition that experimentizes: total length (TL), body long (BL), fork long (FL), height (BH), body weight (BW) and tail vein blood 3 each 2ml of pipe etc.
Two water fillings, put into mullet and pressure and regulate
1 opens water inlet 1, inlet valve 3, water filling in resistance to compression carrier case 6, depth of water BH+10cm.
2 close inlet valve 3, open and shut after perspective window 7 is put into mullet.
3 open the delivery port 11 at 40m place 40, calculating gained bottom half pressure by P=ρ gh is P=1000 * 9.8 * 40=3.92 * 10 5Handkerchief.
4 open valve 3, are filled to 40m.From pressure gage 5 pin-point readings, this value is the accurate pressure values in 6 ends of resistance to compression carrier case.
5 adjustment water inlets, 1 water intake velocity makes it to go out water speed less than serial delivery port 11 maximums, guarantees stable the stable of casing pressure that reach of linker height.
  
Three experimental data collections
1 bait gets into resistance to compression carrier case 6 by water inlet with current, observes the mullet situation of in casing, ingesting.
2 observe mullets in the injecting process and during 40m stress behavior can be with adaptive behavior through 7 serial 7 1, 7 2, 7 37 iThe perspective window is observed.
4 experiments were closed inlet valve 3 after 20 days, opened serial delivery port 11 1, and then open inlet valve 3, make the water body height reduce to serial delivery port 11 1Height.
5 regulate the casing depth of water to BH+10cm once more by discharge outlet 9, take out mullet, and the back image data is handled in experiment: total length (TL 1), the long (BL of body 1), the long (FL of fork 1), height (BH 1), body weight (BW 1) and tail vein blood 3 each 2ml of pipe, lethality, survival rate, fish glue functional check etc.
  
Four adjustment pressure are the 200m depth of water and relevant data
The adjustment of 1 experimental session pressure
Water inlet 1 stops into water and closes inlet valve 3 again, then closes the 40m place and goes out serial delivery port 11 40, open the serial delivery port 11 in 200m place 200, calculating gained bottom half pressure by P=ρ gh is P=1000 * 9.8 * 200=19.6 * 10 5Handkerchief.
2 repeated for the 3rd step, finished until experiment.
Five cleaning systems:
Water inlet 1 stops into water, opens inlet valve 3, opens discharge outlet 9 again, and water is discharged through discharge outlet 9 in the water inlet pipe.
Cleaning resistance to compression carrier case 6 is removed excreta, and desilting etc. are discharged through discharge outlet 9.
Close water inlet 1, inlet valve 3, discharge outlet 9 then, next time, experiment was for use.
  
Instance acipenser dabryanus 80m in 34 age and 140m depth of water pressure intensity testing
Preparation before one experiment
1 water inlet, delivery port, valve are in closed condition.
2 pairs of acipenser dabryanus preceding data acquisition that experimentizes: total length (TL), body long (BL), fork long (FL), height (BH), body weight (BW) and tail vein blood 3 each 2ml of pipe etc.
Two water fillings, put into acipenser dabryanus and pressure and regulate
1 opens water inlet 1 and inlet valve 3, water filling in resistance to compression carrier case 6, depth of water BH+10cm.
2 close inlet valve 3, open and shut after perspective window 7 is put into acipenser dabryanus.
3 open the serial delivery port 11 at 80m place 80, calculating gained bottom half pressure by P=ρ gh is P=1000 * 9.8 * 80=7.84 * 10 5Handkerchief.
4 open inlet valve 3, are filled to 80m, and from pressure gage 5 pin-point readings, this value is the accurate pressure values in 6 ends of resistance to compression carrier case.
5 adjustment water inlets, 1 water intake velocity makes it to go out water speed less than serial delivery port 11 maximums, guarantees stable the stable of casing pressure that reach of linker height.
  
Three experimental data collections
1 bait gets into resistance to compression carrier case by water inlet 1 with current, observes the acipenser dabryanus situation of in casing, ingesting.
2 observe acipenser dabryanus in the injecting process and during 80m stress behavior can be with adaptive behavior through 7 serial 7 1, 7 2, 7 37 iThe perspective window is observed.
4 experiments are after 20 days, and valve-off 3 opens 11 1And then open valve 3, make the water body height reduce to 11 1The delivery port height.
5 regulate the casing depth of water to BH+10cm once more by valve 9, take out acipenser dabryanus, and the back image data is handled in experiment: total length (TL 1), the long (BL of body 1), the long (FL of fork 1), height (BH 1), body weight (BW 1) and tail vein blood 3 each 2ml of pipe, lethality, survival rate, fish glue functional check etc.
  
Four adjustment pressure are the 140m depth of water and relevant data
The adjustment of 1 experimental session pressure
Water inlet 1 stops into water valve-off 3 again, then closes 80m place delivery port 11 80, open 140m place delivery port 11 in downward corresponding height position 140, calculating gained bottom half pressure by P=ρ gh is P=1000 * 9.8 * 140=13.72 * 10 5Handkerchief.
2 repeated for the 3rd step, finished until experiment.
Five cleaning systems
Water inlet 1 stops into water, opens inlet valve 3, opens discharge outlet 9 again, and water is discharged through discharge outlet 9 in the water inlet pipe.
Cleaning resistance to compression carrier case 6 is removed excreta, and desilting etc. are discharged through discharge outlet 9.
Close water inlet 1, inlet valve 3, discharge outlet 9 then, next time, experiment was for use.
Experimental provision of the present invention and method can realize the hydrostatic test under the various pressure of all aquatiles, because length is limited, embodiment enumerates no longer one by one, all belong to the scope of requirement protection of the present invention.
  

Claims (4)

1. the utilization law of connected vessels is realized the device of aquatile hydrostatic test; Comprise water inlet (1), water inlet pipe (2), inlet valve (3), outlet pipe (10); It is characterized in that: the dual-side of resistance to compression carrier case (6) is equipped with water inlet pipe (2) and outlet pipe (10); Have perspective window (7) on the resistance to compression carrier case (6), on the perspective window (7) pressure gage (5) is housed, resistance to compression carrier case (6) bottom has discharge outlet (9); One row's serial apopore (11) is arranged on the outlet pipe (10), and water inlet pipe (2) is equipped with inlet valve (3) near the inside of resistance to compression carrier case (6).
2. utilization law of connected vessels according to claim 1 is realized the device of aquatile hydrostatic test, and it is characterized in that: water inlet pipe screen pack (4) is equipped with at the position that water inlet pipe (2) contacts with resistance to compression carrier case (6).
3. utilization law of connected vessels according to claim 1 is realized the device of aquatile hydrostatic test, and it is characterized in that: outlet pipe screen pack (8) is equipped with at the position that outlet pipe (10) contacts with resistance to compression carrier case (6).
4. utilize the utilization law of connected vessels to realize that the device of aquatile hydrostatic test carries out aquatile hydrostatic test method, is characterized in that:
The step of this method is following:
One, preparation before the experiment: water inlet, delivery port, valve are in closed condition, and carry out the data acquisition that biology is measured, biochemical sample is sampled to testing biology;
Two, experimentize: open inlet valve (3);, water inlet (1) closes inlet valve (3) after injecting the depth of water greater than the organism height; Open the perspective window (7) on the resistance to compression carrier case (6); It is biological to put into experiment, opens in the corresponding serial delivery port (11) of experiment desired height h 11 i, 11 Close Alls of serial delivery port below this height h calculate pressure P by P=ρ gh; Open inlet valve (3); Be filled to experiment desired height h, height is series delivery port 11 places mark at the outlet pipe place, from pressure gage (5), reads actual pressure at the bottom of the resistance to compression carrier case; Adjustment water inlet (1) water intake velocity makes it to go out water speed less than serial delivery port (11) maximum;
Three experimental data collections: bait gets into resistance to compression carrier case (6) by water inlet (1) with current, through the adaptive behavior of perspective window (7) observation experiment biology to pressure, after experiment finishes; Close inlet valve (3); Open the little discharge outlet of serial delivery port (11) lowest order, when water level reaches this discharge outlet height, regulate once more the casing depth of water to organism highly quite after; It is biological to take out experiment, image data;
The clean system of cleaning politics, economics, organization, and ideology: water inlet (1) stops into water; Open inlet valve (3) and discharge outlet (9), with the water emptying, cleaning resistance to compression carrier case (6); Close water inlet (1), serial delivery port (11), inlet valve (3) then, use in order to experiment next time.
CN201210127270XA 2012-04-27 2012-04-27 Method for realizing hydrostatic tests of aquatic organisms based on principle of communicating vessels Expired - Fee Related CN102657116B (en)

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Publication number Priority date Publication date Assignee Title
JPH0315332A (en) * 1989-06-13 1991-01-23 Nkk Corp High pressure water tank for rearing organism
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JPH03224426A (en) * 1990-01-31 1991-10-03 Kaiyo Kagaku Gijutsu Center High pressure experimental water-tank
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CN201557452U (en) * 2009-09-25 2010-08-25 湛江国联水产开发股份有限公司 Shrimp culturing high-elevation pond
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CN201860647U (en) * 2010-10-18 2011-06-15 嘉兴学院 Fish tank capable of automatically changing water and feeding
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CN202618019U (en) * 2012-04-27 2012-12-26 中国水产科学研究院长江水产研究所 Device for realizing aquatic organism water pressure experiment by using principle of communicating vessels

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李海锋等: "负压状态下压力变化导致鲫鱼身体组织的损伤", 《动物学报》, vol. 49, 15 February 2003 (2003-02-15), pages 67 - 72 *

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