CN102653784B - 用于多重核酸测序的标签及其使用方法 - Google Patents
用于多重核酸测序的标签及其使用方法 Download PDFInfo
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- CN102653784B CN102653784B CN201110050238.1A CN201110050238A CN102653784B CN 102653784 B CN102653784 B CN 102653784B CN 201110050238 A CN201110050238 A CN 201110050238A CN 102653784 B CN102653784 B CN 102653784B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
Abstract
Description
试剂名称 | 货号 |
10mM dNTP Mix(10mM dNTP混合液) | part#1000564 |
DNA Polymerase I(DNA聚合酶I) | part#1000577 |
5×T4DNA Ligase Buffer(5×T4DNA连接酶缓冲液) | part#1000581 |
T4DNA Ligase(T4DNA连接酶) | part#1000580 |
10×Restriction Buffer(10×限制性酶切缓冲液) | part#1000583 |
5×Phusion HF Buffer(5×Phusion高保真酶缓冲液) | part#1000585 |
2×Phusion Polymerase(Phusion高保真酶) | part#1000584 |
25mM dNTP Mix(25mM dNTP混合液) | part#1001663 |
25bp Ladder | part#1001662 |
10×Gel Elution Buffer(10×溶胶缓冲液) | part#1000571 |
Resuspension Buffer(溶解缓冲液) | part#1001388 |
Sera-mag Magnetic Oligo(dT)Beads(Oligo(dT)磁珠) | part#1002545 |
Ultra Pure Water(超纯水) | part#1000467 |
Paired-End DNA Sample Prep Kit | IP-102-1001 |
10x Polynucleotide Kinase Buffer | B904(Enzymatics) |
10x blue buffer | B011(Enzymatics) |
dATP | P0756L(NEB) |
2x Rapid ligation buffer | B101(Enzymatics) |
Index PE Adapter Oligo Mix |
来自步骤2的样品 | 30μl |
Ultra Pure Water(超纯水) | 45uL |
10x Polynucleotide Kinase Buffer | 10μl |
10mM dNTP Mix | 4μl |
T4DNA Polymerase | 5μl |
Klenow Fragment | 1μl |
T4Polynucleotide Kinase | 5μl |
总体积 | 100μl |
来自步骤3的样品 | 32μl |
10x blue buffer | 5μl |
dATP | 10μl |
Klenow(3’-5’exo-) | 3μl |
总体积 | 50μl |
来自步骤4的样品 | 10μl |
2x Rapid ligation buffer | 25μl |
PE Index Adapter Oligo Mix | 10μl |
T4DNA Ligase | 5μl |
总体积 | 50μl |
来自步骤6的样品 | 10μl |
Phusion DNA Polymerase | 25μl |
PCRprimer 1 | 1μl |
PCRprimer 2 | 1μl |
UltraPureTM Water | 13μl |
总体积 | 50μl |
Claims (30)
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201110050238.1A CN102653784B (zh) | 2011-03-03 | 2011-03-03 | 用于多重核酸测序的标签及其使用方法 |
PCT/CN2012/071893 WO2012116661A1 (zh) | 2011-03-03 | 2012-03-02 | Dna标签及其应用 |
HK13102235.6A HK1175208A1 (zh) | 2011-03-03 | 2013-02-21 | 用於多重核酸測序的標簽及其使用方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201110050238.1A CN102653784B (zh) | 2011-03-03 | 2011-03-03 | 用于多重核酸测序的标签及其使用方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102653784A CN102653784A (zh) | 2012-09-05 |
CN102653784B true CN102653784B (zh) | 2015-01-21 |
Family
ID=46729514
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201110050238.1A Active CN102653784B (zh) | 2011-03-03 | 2011-03-03 | 用于多重核酸测序的标签及其使用方法 |
Country Status (3)
Country | Link |
---|---|
CN (1) | CN102653784B (zh) |
HK (1) | HK1175208A1 (zh) |
WO (1) | WO2012116661A1 (zh) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102978205B (zh) * | 2012-11-19 | 2014-08-20 | 北京诺禾致源生物信息科技有限公司 | 一种应用于标记开发的高通量测序的接头及其运用方法 |
EP3077543B1 (en) * | 2013-12-05 | 2019-09-25 | Centrillion Technology Holdings Corporation | Fabrication of patterned arrays |
CN103902852B (zh) * | 2014-03-21 | 2017-03-22 | 深圳华大基因科技有限公司 | 基因表达的定量方法及装置 |
CN104357918B (zh) * | 2014-11-25 | 2016-08-17 | 北京阅微基因技术有限公司 | 血浆游离dna文库的构建方法 |
CN105401222A (zh) * | 2015-12-30 | 2016-03-16 | 安诺优达基因科技(北京)有限公司 | 一种构建测序用dna文库的方法 |
CN105483267B (zh) * | 2016-01-15 | 2018-12-04 | 古博 | 血浆游离DNA双分子标记、标记和检测血浆cfDNA的方法及其用途 |
CN105506748B (zh) * | 2016-01-18 | 2018-11-27 | 北京百迈客生物科技有限公司 | 一种dna高通量测序建库方法 |
CN106995836B (zh) * | 2016-01-22 | 2020-08-25 | 益善生物技术股份有限公司 | 二代测序样品前处理的引物和方法以及试剂盒 |
US11674137B2 (en) * | 2016-05-27 | 2023-06-13 | Haplox Biotechnology (Shenzhen) Co., Ltd. | Adaptor for sequencing DNA at ultratrace level and use thereof |
CN106047861A (zh) * | 2016-06-07 | 2016-10-26 | 苏州贝斯派生物科技有限公司 | 一种快速修复损伤dna的试剂、及其制备方法与应用 |
CN107475352A (zh) * | 2016-06-08 | 2017-12-15 | 深圳华大基因股份有限公司 | 一种用于边合成边测序的测序仪的通用型pcr扩增融合引物 |
CN109996877A (zh) * | 2016-12-16 | 2019-07-09 | 深圳华大基因股份有限公司 | 一种用于核酸样品标识的基因标签、试剂盒及其应用 |
WO2018217625A1 (en) * | 2017-05-23 | 2018-11-29 | Bio-Rad Laboratories, Inc. | Molecular barcoding |
CN109321984B (zh) * | 2017-08-01 | 2022-08-23 | 浙江安诺优达生物科技有限公司 | 一种测序用dna文库 |
CN110468188B (zh) * | 2019-08-22 | 2023-08-22 | 广州微远医疗器械有限公司 | 用于二代测序的标签序列集及其设计方法和应用 |
CN111235244A (zh) * | 2019-11-28 | 2020-06-05 | 广州微远基因科技有限公司 | 测序内标分子及其制备方法和应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003052137A2 (de) * | 2001-12-19 | 2003-06-26 | Gnothis Holding Sa | Evaneszenz-basierendes multiplex-sequenzierungsverfahren |
CN101921840A (zh) * | 2010-06-30 | 2010-12-22 | 深圳华大基因科技有限公司 | 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10356783A1 (de) * | 2003-12-02 | 2005-07-07 | Universität Zu Köln | Verfahren zur Multiplex-Sequenzierung |
US20060263789A1 (en) * | 2005-05-19 | 2006-11-23 | Robert Kincaid | Unique identifiers for indicating properties associated with entities to which they are attached, and methods for using |
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2011
- 2011-03-03 CN CN201110050238.1A patent/CN102653784B/zh active Active
-
2012
- 2012-03-02 WO PCT/CN2012/071893 patent/WO2012116661A1/zh active Application Filing
-
2013
- 2013-02-21 HK HK13102235.6A patent/HK1175208A1/zh unknown
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003052137A2 (de) * | 2001-12-19 | 2003-06-26 | Gnothis Holding Sa | Evaneszenz-basierendes multiplex-sequenzierungsverfahren |
CN101921840A (zh) * | 2010-06-30 | 2010-12-22 | 深圳华大基因科技有限公司 | 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法 |
Non-Patent Citations (1)
Title |
---|
highly-multiplexed barcode sequencing: an effecient method for parallel analysis of pooled samples;andrew m. smith et al.;《nucleic acids research》;20100511;第38卷(第13期);e142 * |
Also Published As
Publication number | Publication date |
---|---|
WO2012116661A1 (zh) | 2012-09-07 |
HK1175208A1 (zh) | 2013-06-28 |
CN102653784A (zh) | 2012-09-05 |
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