CN102653477A - Preparation method of vinasse-type bio-organic fertilizer - Google Patents
Preparation method of vinasse-type bio-organic fertilizer Download PDFInfo
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- CN102653477A CN102653477A CN2012101450603A CN201210145060A CN102653477A CN 102653477 A CN102653477 A CN 102653477A CN 2012101450603 A CN2012101450603 A CN 2012101450603A CN 201210145060 A CN201210145060 A CN 201210145060A CN 102653477 A CN102653477 A CN 102653477A
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- aspergillus fumigatus
- vinasse
- organic fertilizer
- seed culture
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
The invention discloses a preparation method of a vinasse-type bio-organic fertilizer, comprising the following steps of: (1) preparation of an aspergillus fumigatus seed culture solution, which comprises a, preparing a seed culture medium, b, revivifying seeds, c, activating the seeds and d, carrying out spread culture on the seeds; and (2) preparation of a bio-organic fertilizer, which comprises uniformly mixing vinasse and water in a mass ratio of (1: 1)-(1: 2) to obtain a culture medium matrix, inoculating 2-6% of aspergillus fumigatus seed culture solution in a liquid-solid ratio ml/g, and culturing for 4-7 days with a ventilatory capacity of 15-30% at the temperature of 42-48 DEG C. The vinasse-type bio-organic fertilizer obtained by using the preparation method disclosed by the invention is short in production cycle, less in odour and high in utilization rate.
Description
Technical field
The present invention relates to technical field of fertilizers, specifically come just to relate to a kind of preparation method of biological organic fertilizer.
Background technology
Solid white spirit waste lees (being vinasse) is the main by product that liquor is produced, and also is the principal pollutant that liquor is produced.According to statistics, the 4-8 that loses poor generation and be liquor output doubly, only China needs poor the reaching more than 5,000 ten thousand tons of losing of handling at present.Therefore, the comprehensive utilization of vinasse has crucial meaning to development of resources and environment protection.On the one hand because the general raw materials such as Chinese sorghum, wheat that adopt, lose and contain rich in protein, amino acid, VITAMINs and various trace elements etc. in poor, for the comprehensive utilization of solid white spirit waste lees provides good condition.Because production technique is special, lose poor acidity height on the other hand, be prone to corrupt going bad, if untimely processing, must serious environment pollution.According to the characteristics of vinasse, people have carried out many-sided research to its recycling.At present, the vinasse as fertilizer sources generally adopts the method for nature compost, has characteristics such as long reaction time, stink is big, utilization ratio is low.
Aspergillus fumigatus (
Aspergillus fumigatus), CICC 2434, are preserved in Chinese industrial microbial strains preservation center, and decomposition of cellulose, semicellulose have the advantage that vitality is strong, degradation effect is outstanding concurrently simultaneously.
Summary of the invention
The preparation method of a kind of with short production cycle, stink is little, utilization ratio the is high vinasse type biological organic fertilizer that the objective of the invention is to overcome above-mentioned shortcoming and provide.
A kind of preparation method of vinasse type biological organic fertilizer comprises that step is following:
(1) Aspergillus fumigatus (
Aspergillus fumigatus) preparation of seed culture fluid
A, seed culture medium preparation
Potato glucose liquid nutrient medium: yam 200g, glucose 20g, tap water 1000ml, pH nature; Method for making: behind the peeling potatoes, be cut into small pieces, add poach mashed (boil 20 ~ 30min, can be poked get final product) by glass stick, with 4 layers of filtered through gauze, sugaring and agar again, after the heating and melting restock moisture to 1000ml, the 121 ℃ times 20min that sterilize;
Potato glucose solid medium: yam 200g, glucose 20g, agar 15 ~ 20g, tap water 1000ml, pH nature; Method for making: behind the peeling potatoes, be cut into small pieces, add poach mashed (boil 20 ~ 30min, can be poked get final product) by glass stick, with 4 layers of filtered through gauze, sugaring and agar again, after the heating and melting restock moisture to 1000ml, the 121 ℃ times 20min that sterilize;
B, seed bring back to life
Aspergillus fumigatus is seeded to the potato glucose solid medium in gnotobasis, static cultivation 72h under 45 ℃ of constant temperatures;
C, seed activation
Aspergillus fumigatus after bringing back to life is seeded to the potato glucose solid medium, static cultivation 72h under 45 ℃ of constant temperatures under gnotobasis;
D, seed spread cultivation
Bacterial classification after the activation is encircled to the potato glucose liquid nutrient medium with inoculation articulating 3 ~ 5 in gnotobasis, after cultivating 72h under 45 ℃ of constant temperatures, be the Aspergillus fumigatus seed culture fluid;
(2) preparation of biological organic fertilizer: by vinasse and quality than 1:1 ~ 1:2 mix medium matrix, by the Aspergillus fumigatus seed culture fluid of liquid-solid ratio ml/g inoculation 2 ~ 6%, air flow 15 ~ 30% is cultivated down for 42 ~ 48 ℃ and was got final product in 4 ~ 7 days.
The present invention compared with prior art has tangible beneficial effect, can know from above technical scheme: select for use Aspergillus fumigatus (
Aspergillus fumigatus) vinasse are carried out pre-treatment, can make Mierocrystalline cellulose in the vinasse, semicellulose be able to degraded, shorten the production cycle of vinasse type biological organic fertilizer, to the comprehensive utilization of vinasse favourable condition is provided.
Embodiment
Embodiment 1
A kind of preparation method of vinasse type biological organic fertilizer comprises that step is following:
(1) Aspergillus fumigatus (
Aspergillus fumigatus) preparation of seed culture fluid
A, seed culture medium preparation
Potato glucose liquid nutrient medium: yam 200g, glucose 20g, tap water 1000ml, pH nature; Method for making: behind the peeling potatoes, be cut into small pieces, add poach mashed (boil 20 ~ 30min, can be poked get final product) by glass stick, with 4 layers of filtered through gauze, sugaring and agar again, after the heating and melting restock moisture to 1000ml, the 121 ℃ times 20min that sterilize;
Potato glucose solid medium: yam 200g, glucose 20g, agar 15 ~ 20g, tap water 1000ml, pH nature; Method for making: behind the peeling potatoes, be cut into small pieces, add poach mashed (boil 20 ~ 30min, can be poked get final product) by glass stick, with 4 layers of filtered through gauze, sugaring and agar again, after the heating and melting restock moisture to 1000ml, the 121 ℃ times 20min that sterilize;
B, seed bring back to life
To purchase in Chinese industrial microbial strains preservation center Aspergillus fumigatus (
Aspergillus fumigatus), CICC 2343, in gnotobasis, are seeded to the potato glucose solid medium, static cultivation 72h under 45 ℃ of constant temperatures;
C, seed activation
Aspergillus fumigatus after bringing back to life is seeded to the potato glucose solid medium, static cultivation 72h under 45 ℃ of constant temperatures under gnotobasis;
D, seed spread cultivation
Bacterial classification after the activation is encircled to the potato glucose liquid nutrient medium with inoculation articulating 3 ~ 5 in gnotobasis, after cultivating 72h under 45 ℃ of constant temperatures, be the Aspergillus fumigatus seed culture fluid;
(2) preparation of biological organic fertilizer:
Take by weighing the 30g vinasse, add entry 45g, inoculation 3ml Aspergillus fumigatus seed culture fluid stirs, air flow 20% (liquid level accounts for the volume of whole fermenting container), and 42 ℃ of bottom fermentations promptly got in 4 days.
Measure pH value in the gained biological organic fertilizer, E4/E6 value, ammonia-state nitrogen, organic matter, germination index as follows:
1. the mensuration of pH
Biological organic fertilizer with the deionized water meat dishes to go with liquor poor with water than 1:10 (W (g): V (ml)), at room temperature, behind level vibration 1h under the 200r/min, filter paper filtering is drawn 20ml filtrating, directly measures the pH value with pH meter.
2. humic acids E4/E6
Draw above-mentioned filtrating 20ml, be determined at 465nm and 665nm place ground absorbancy respectively, ratio between two is the E4/E6 value.
3. the mensuration of ammonia-state nitrogen
Adopt FTM to measure wherein ammonia nitrogen content (GB/T 3600-2000).
4. the mensuration of organic content
Adopt calcination method (weight-loss method of burning) to measure organic content in the vinasse.
Theoretically, GI < 10 just judge that phytotoxicity, Gl are arranged>50 just thinks that compost is own through becoming thoroughly decomposed and having reached the acceptable degree.
Each achievement data sees the following form
Embodiment 2,
A kind of preparation method of vinasse type biological organic fertilizer comprises that step is following:
(1) Aspergillus fumigatus (
Aspergillus fumigatus) preparation of seed culture fluid
With embodiment 1;
(2) preparation of biological organic fertilizer:
Take by weighing the 4500g vinasse, add entry 9000g, inoculation 200ml Aspergillus fumigatus seed culture fluid, air flow 25% (liquid level accounts for the volume of whole fermenting container), 48 ℃ of bottom fermentations promptly got in 7 days.
The method of pressing among the embodiment 1 is measured pH value, E4/E6 value, ammonia-state nitrogen, organic matter in the gained biological organic fertilizer.
Each achievement data sees the following form
Claims (2)
1. the preparation method of a vinasse type biological organic fertilizer comprises that step is following:
(1) preparation of Aspergillus fumigatus seed culture fluid
A, seed culture medium preparation
Potato glucose liquid nutrient medium: yam 200g, glucose 20g, tap water 1000ml, pH nature; Method for making: behind the peeling potatoes, be cut into small pieces, it is mashed to add poach, with 4 layers of filtered through gauze, sugaring and agar again, after the heating and melting restock moisture to 1000ml, 121 ℃ of 20min that sterilize down;
Potato glucose solid medium: yam 200g, glucose 20g, agar 15 ~ 20g, tap water 1000ml, pH nature; Method for making: behind the peeling potatoes, be cut into small pieces, it is mashed to add poach, with 4 layers of filtered through gauze, sugaring and agar again, after the heating and melting restock moisture to 1000ml, 121 ℃ of 20min that sterilize down;
B, seed bring back to life
Aspergillus fumigatus is seeded to the potato glucose solid medium in gnotobasis, static cultivation 72h under 45 ℃ of constant temperatures;
C, seed activation
Aspergillus fumigatus after bringing back to life is seeded to the potato glucose solid medium, static cultivation 72h under 45 ℃ of constant temperatures under gnotobasis;
D, seed spread cultivation
Bacterial classification after the activation is encircled to the potato glucose liquid nutrient medium with inoculation articulating 3 ~ 5 in gnotobasis, after cultivating 72h under 45 ℃ of constant temperatures, be the Aspergillus fumigatus seed culture fluid;
(2) preparation of biological organic fertilizer: by vinasse and quality than 1:1 ~ 1:2 mix medium matrix, by the Aspergillus fumigatus seed culture fluid of liquid-solid ratio ml/g inoculation 2 ~ 6%, air flow 15 ~ 30% is cultivated down for 42 ~ 48 ℃ and was got final product in 4 ~ 7 days.
2. the preparation method of vinasse type biological organic fertilizer as claimed in claim 1 wherein adds mashed being meant of poach and boils 20 ~ 30min, can be poked by glass stick to get final product.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012101450603A CN102653477B (en) | 2012-05-11 | 2012-05-11 | Preparation method of vinasse-type bio-organic fertilizer |
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| CN2012101450603A CN102653477B (en) | 2012-05-11 | 2012-05-11 | Preparation method of vinasse-type bio-organic fertilizer |
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| CN102653477B CN102653477B (en) | 2013-12-25 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102936159A (en) * | 2012-11-01 | 2013-02-20 | 青岛农业大学 | Method for producing compost by using beer production waste |
| CN104829328A (en) * | 2015-04-20 | 2015-08-12 | 唐兴龙 | Special fertilizer used for increasing grape sugar content |
| CN107011009A (en) * | 2017-04-26 | 2017-08-04 | 陕西枫丹百丽生物科技有限公司 | A kind of spirit stillage organic fertilizer and its preparation method and application |
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| CN1609064A (en) * | 2004-09-03 | 2005-04-27 | 邹启吾 | Process of preparing active protein powder and organic fertilizer with distiller's residue |
| CN101255076A (en) * | 2008-03-20 | 2008-09-03 | 邹启吾 | Grain stillage organic compound fertilizer and method for producing organic fertilizer |
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| CN102077903A (en) * | 2010-11-25 | 2011-06-01 | 河南农业大学 | Method for jointly treating stalks by steam explosion and microorganism fermentation |
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2012
- 2012-05-11 CN CN2012101450603A patent/CN102653477B/en not_active Expired - Fee Related
Patent Citations (5)
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| CN1119495A (en) * | 1994-09-15 | 1996-04-03 | 四川省宜宾五粮液酒厂科研所 | Method for producing active protein feedstuff by using distillers' grains |
| CN1609064A (en) * | 2004-09-03 | 2005-04-27 | 邹启吾 | Process of preparing active protein powder and organic fertilizer with distiller's residue |
| CN101255076A (en) * | 2008-03-20 | 2008-09-03 | 邹启吾 | Grain stillage organic compound fertilizer and method for producing organic fertilizer |
| CN101946851A (en) * | 2010-09-20 | 2011-01-19 | 河南普爱饲料股份有限公司 | Biological protein feed, preparation method thereof and using method thereof |
| CN102077903A (en) * | 2010-11-25 | 2011-06-01 | 河南农业大学 | Method for jointly treating stalks by steam explosion and microorganism fermentation |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102936159A (en) * | 2012-11-01 | 2013-02-20 | 青岛农业大学 | Method for producing compost by using beer production waste |
| CN102936159B (en) * | 2012-11-01 | 2014-07-30 | 青岛农业大学 | Method for producing compost by using beer production waste |
| CN104829328A (en) * | 2015-04-20 | 2015-08-12 | 唐兴龙 | Special fertilizer used for increasing grape sugar content |
| CN107011009A (en) * | 2017-04-26 | 2017-08-04 | 陕西枫丹百丽生物科技有限公司 | A kind of spirit stillage organic fertilizer and its preparation method and application |
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| CN102653477B (en) | 2013-12-25 |
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Application publication date: 20120905 Assignee: Guizhou Jane Wine Co.,Ltd. Assignor: Guizhou University Contract record no.: 2014520000005 Denomination of invention: Preparation method of vinasse-type bio-organic fertilizer Granted publication date: 20131225 License type: Exclusive License Record date: 20140326 |
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