CN102648733A - Composite microorganism preparation affecting growth and immunity of stichopus japonicus - Google Patents
Composite microorganism preparation affecting growth and immunity of stichopus japonicus Download PDFInfo
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- CN102648733A CN102648733A CN2011100441975A CN201110044197A CN102648733A CN 102648733 A CN102648733 A CN 102648733A CN 2011100441975 A CN2011100441975 A CN 2011100441975A CN 201110044197 A CN201110044197 A CN 201110044197A CN 102648733 A CN102648733 A CN 102648733A
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- stichopus japonicus
- bacillus subtilis
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Abstract
The invention discloses a composite microorganism preparation affecting growth and immunity of stichopus japonicus. The composite microorganism preparation comprises bacillus subtilis, licheniformis bacillus and carrier, and is characterized by comprising the following components in percentage by weight: 30-35 percent of bacillus subtilis, 15-20 percent of licheniformis bacillus and 50-55 percent of carrier. So long as 0.1-1 percent of composite microorganism is added in the stichopus japonicus, the growth of the stichopus japonicus can be remarkably promoted, the production efficiency is advanced, and no side effect is generated, so that the planting benefit is improved.
Description
Technical field
The present invention relates to a kind of microorganism formulation of growing of promoting, particularly a kind of complex microorganism preparations that stichopus japonicus growth and immunity are influenced.
Background technology
Stichopus japonicus is the good merchantable brand of tonifying Qi health, and its form is cylindric, and front port Zhousheng has 20 feelers, and there is the capable corn of 4-6 at the back side, and the outside of belly has 3 administration-managements foots, yellowish-brown, dark brown, green and brown, the pure white or black and white of body colour etc.Stichopus japonicus is the superfine product among the sea cucumber, is distributed in the Huanghai Sea and the marine site, the Bohai Sea of China more, and promptly in China Liaoning and offshore sea waters, Shandong, owing to have high economic worth, the breed of stichopus japonicus has become the important part of holothruian cultures.
Existing cultural method has following characteristics: the one, and cultural technique is simple, and pond, stone throwing culture, shallow seabed sowing are cultured and all can at the bottom of the mud.The 2nd, market prospects are wide, and sea cucumber nutrient is worth high, and supply falls short of demand in present market, anxious market.The 3rd, high efficiency.With the Yantai City, Shandong Province is example, and the annual every mu of culture benefit in this city is all more than 20,000 yuan, and the benefit that other areas have can reach 30,000 yuan.Daily management also than being easier to, is changed water, adopts freely to receive damp formula and culture, and guarantee to receive every day twice in water.Disease appears in quantity of exchanged water less easily.Stichopus japonicus is narrow to the accommodation of temperature, between 15-22, when temperature surpasses 28 ℃, need take certain measure cooling.Feeding and management at ordinary times is then comparatively simple, and stichopus japonicus mainly is to be food with planktonic organism, benthic diatom, organic debris, and the food organisms in the seawater can be satisfied their growing requirements basically, in planktonic organism few season, and an amount of feed of can throwing something and feeding.
Except above-mentioned basic cultural technique,, reach the effect that promotes growth if microorganism formulation can in breeding environment, be added; To save cost greatly, and microorganism itself has the function of improving water body, will make water body environment good; Can polluted-water, reduced the cost of water treatment.
Summary of the invention
For solving the problems of the technologies described above, the present invention provides a kind of complex microorganism preparations to stichopus japonicus growth and immunity influence, and the amount that only needs to add 0.1%-1% just can promote the growth of stichopus japonicus significantly, greatly reduces cost, has improved production efficiency.
The present invention realizes through following technical scheme:
A kind of complex microorganism preparations to stichopus japonicus growth and immunity influence comprises bacillus subtilis, lichengermium and carrier, is bacillus subtilis 30%～35% according to the quality proportioning, lichengermium 15%～20%, and carrier 50%～55% is mixed and made into.
Said complex microorganism preparations is selected 40～200 purpose powder.
Said bacillus subtilis is the mixture of bacillus subtilis SZ02-1 and bacillus subtilis SZ08-7, and wherein bacillus subtilis SZ02-1 accounts for 25%～28%.
Said bacillus subtilis SZ02-1 is 2-10 * 10
8CFU/g, bacillus subtilis SZ08-7 are 1-5 * 10
8CFU/g.
Said lichengermium is lichengermium SZ11-7.
Said lichengermium is 5-15 * 10
8CFU/g.
Said carrier is a talcum powder.
Beneficial effect of the present invention is: can directly drop in the water body of apostichopus japonicus culture, dissolubility is good, and promotes that growth result is high; Only the amount of needs 1% just can promote growth significantly, has reduced to production cost and cycle greatly, has improved production efficiency; The present invention has also increased the immunity of stichopus japonicus; Its death rate also being reduced, and can improve water body, is Green Product.
Description of drawings
Fig. 1 be randomly draw two kinds of sample treatment stichopus japonicus first body weight and 60 days the opisthosoma anharmonic ratio, and with not through the sample of the processing of microorganism formulation of the present invention just body weight and opisthosoma heavily compare, (1 and 2 represent 0.1% and 1% interpolation level respectively.)
Fig. 2 is the comparison of randomly drawing the relative weight gain rate of two kinds of sample treatment stichopus japonicus, and and with the comparison of the relative weight gain rate of the sample of the processing of not passing through microorganism formulation of the present invention, (1 and 2 represent 0.1% and 1% interpolation level respectively.)
The specific embodiment
Below in conjunction with embodiment and experiment test, the present invention is further specified.
Embodiment 1
According to mass ratio is the mixture 30% of bacillus subtilis SZ02-1 and bacillus subtilis SZ08-7, and wherein bacillus subtilis SZ02-1 accounts for 25%, and clothing gemma bacterium SZ11-7 is 20%, and talcum powder 55% mixes, and wherein bacillus subtilis SZ02-1 is 2 * 10
8CFU/g, bacillus subtilis SZ08-7 are 2 * 10
8CFU/g, lichengermium are 5 * 10
8CFU/g selects 40～200 purpose powder after preparation is accomplished.
Embodiment 2
According to mass ratio is the mixture 35% of bacillus subtilis SZ02-1 and bacillus subtilis SZ08-7, and wherein bacillus subtilis SZ02-1 accounts for 28%, and clothing gemma bacterium SZ11-7 is 20%, and talcum powder 50% mixes, and wherein bacillus subtilis SZ02-1 is 6 * 10
8CFU/g, bacillus subtilis SZ08-7 are 3 * 10
8CFU/g, lichengermium are 10 * 10
8CFU/g selects 40～200 purpose powder after preparation is accomplished.
Embodiment 3
According to mass ratio is the mixture 33% of bacillus subtilis SZ02-1 and bacillus subtilis SZ08-7, and wherein bacillus subtilis SZ02-1 accounts for 26.5%, and clothing gemma bacterium SZ11-7 is 15%, and talcum powder 52% mixes, and wherein bacillus subtilis SZ02-1 is 10 * 10
8CFU/g, bacillus subtilis SZ08-7 are 5 * 10
8CFU/g, lichengermium are 15 * 10
8CFU/g selects 40～200 purpose powder after preparation is accomplished.
The foregoing description is carried out the growth experiment of stichopus japonicus, experiment as follows:
Experimental design:
The foregoing description is made 30 samples, and numbering 1-30 randomly draws two sample number into spectrum 07103 and 07204, and specimen is added the two kinds of additions (0.1% and 1%) in the stichopus japonicus to, and the stichopus japonicus of not adding any microorganism formulation to throw something and feed is as contrast.Each handles 5 repetitions, and each repeats 10 stichopus japonicus.
Duration of experiment 30 days.
Experimentation:
Microorganism formulation admixed in the bait by mass ratio %0.1 and 1% respectively throw something and feed, regularly collect residual bait and the ight soil of stichopus japonicus every day.Measure grazing rate, digestibility and the specific growth rate of stichopus japonicus.
Regularly change water, continue inflation, periodicity of illumination is 12D: 12L.
Get stichopus japonicus body wall, enteron aisle, respiratory trees respectively at when beginning experiment, after feeding 30 days, measure the related immune index.The related immune index mainly comprises: blood count, activate the phagocytic capacity mensuration, respiratory burst vitality test, phenol oxidase vigor (po), ACP (acid phosphatase), AKP (alkaline phosphatase), SOD, catalase, serum lysozyme, CIR etc.
Culture experiment is defended the experiment base and is carried out on Chinese Marine University Ao mountain, all samples is taken back the ecological laboratory of Chinese Marine University and measured.
Experimental result:
After experiment is carried out 30 days, each body weight of handling stichopus japonicus is measured, and calculated the relative weight gain rate (Fig. 1 and Fig. 2).
On the whole, add the growth that microorganism formulation has all promoted stichopus japonicus in various degree.From kind relatively, 07204 short long-acting fruit is better than 07103; And 1% addition is better than 0.1% short long-acting fruit.
Experiment conclusion
30 days preliminary culture experiment result can find out that 07204 1% addition can significantly promote the growth of stichopus japonicus.Promptly the invention belongs to trace and add, only adding, 1% preparation just can efficiently promote the stichopus japonicus growth.
Claims (7)
1. complex microorganism preparations that stichopus japonicus growth and immunity are influenced; Comprise bacillus subtilis, lichengermium and carrier; It is characterized in that according to the quality proportioning be bacillus subtilis 30%～35%, lichengermium 15%～20%, carrier 50%～55% is mixed and made into.
2. the complex microorganism preparations to stichopus japonicus growth and immunity influence as claimed in claim 1 is characterized in that said complex microorganism preparations selection 40～200 purpose powder.
3. the complex microorganism preparations to stichopus japonicus growth and immunity influence as claimed in claim 1; It is characterized in that said bacillus subtilis is the mixture of bacillus subtilis SZ02-1 and bacillus subtilis SZ08-7, wherein bacillus subtilis SZ02-1 accounts for 25%～28%.
4. the complex microorganism preparations to stichopus japonicus growth and immunity influence as claimed in claim 1 is characterized in that said bacillus subtilis SZ02-1 is 2-10 * 10
8CFU/g, bacillus subtilis SZ08-7 are 1-5 * 10
8CFU/g.
5. the complex microorganism preparations to stichopus japonicus growth and immunity influence as claimed in claim 1 is characterized in that said lichengermium is lichengermium SZ11-7.
6. the complex microorganism preparations to stichopus japonicus growth and immunity influence as claimed in claim 1 is characterized in that said lichengermium is 5-15 * 10
8CFU/g.
7. the complex microorganism preparations to stichopus japonicus growth and immunity influence as claimed in claim 1 is characterized in that said carrier is a talcum powder.
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| CN2011100441975A CN102648733A (en) | 2011-02-24 | 2011-02-24 | Composite microorganism preparation affecting growth and immunity of stichopus japonicus |
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| CN2011100441975A CN102648733A (en) | 2011-02-24 | 2011-02-24 | Composite microorganism preparation affecting growth and immunity of stichopus japonicus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108949615A (en) * | 2018-06-25 | 2018-12-07 | 青岛农业大学 | Probiotics for the apostichopus japonicus Selenka feed that ferments |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101904411A (en) * | 2008-05-14 | 2010-12-08 | 北京大北农科技集团股份有限公司 | Composite microbial ecological agent and application thereof |
| CN101928678A (en) * | 2009-06-19 | 2010-12-29 | 陈秀蓉 | Bacillus subtilis B2 microbial agent, compound microbial agent and application |
| CN101971922A (en) * | 2010-07-15 | 2011-02-16 | 大连理工大学 | Environment-friendly sea cucumber streak feed and preparation method thereof |
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2011
- 2011-02-24 CN CN2011100441975A patent/CN102648733A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101904411A (en) * | 2008-05-14 | 2010-12-08 | 北京大北农科技集团股份有限公司 | Composite microbial ecological agent and application thereof |
| CN101928678A (en) * | 2009-06-19 | 2010-12-29 | 陈秀蓉 | Bacillus subtilis B2 microbial agent, compound microbial agent and application |
| CN101971922A (en) * | 2010-07-15 | 2011-02-16 | 大连理工大学 | Environment-friendly sea cucumber streak feed and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108949615A (en) * | 2018-06-25 | 2018-12-07 | 青岛农业大学 | Probiotics for the apostichopus japonicus Selenka feed that ferments |
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Application publication date: 20120829 |