CN102618470A - Scale-up culture method for lactobacillus acidophilus - Google Patents
Scale-up culture method for lactobacillus acidophilus Download PDFInfo
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- CN102618470A CN102618470A CN2012100930904A CN201210093090A CN102618470A CN 102618470 A CN102618470 A CN 102618470A CN 2012100930904 A CN2012100930904 A CN 2012100930904A CN 201210093090 A CN201210093090 A CN 201210093090A CN 102618470 A CN102618470 A CN 102618470A
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Abstract
The invention relates to a scale-up culture method for lactobacillus acidophilus, which is characterized by 1) preparing strains comprising aspergillus niger-spores and the lactobacillus acidophilus in the weight ratio of 8-9:1; 2) preparing a culture medium comprising, in mass percent, 30-35% of rice bran, 15-20% of bean pulp, 0.8-1% of NaCl and 44-54.2% of bran, and uniformly stirring the components after mixing; and 3) performing solid fermentation, pouring the culture medium into a solid fermentation tank, adding the aspergillus niger-spores accounting for 0.05-0.1% of the weight of the culture medium, proportionally adding water, continuously supplying filtered air, inoculating the lactobacillus acidophilus accounting for 1% of the weight of the culture medium after performing fermentation for 24 hours, keeping relative humidity and air supply quantity unchanged and then performing fermentation for 48 hours again. The scale-up culture method realizes industrial solid fermentation and low-cost scale-up culture and has the advantages of low cost and high production efficiency.
Description
Technical field
The present invention relates to the large-scaled culture method of a kind of Lactobacterium acidophilum, belong to the field of agricultural planting fertilizer, especially microbial preparation culture technique field.
Background technology
Lactobacterium acidophilum is a member important in the milk-acid bacteria family, belongs to the Gram-positive bacillus of lactobacillus, extensively is present in the enteron aisle of animal.Lactobacterium acidophilum can secrete antibiotin class material (acidolin (acidolin), bacillus acidophilus plain (acidophilin), lactobacillin (laetocidon)); Pathogen enterobacteria is produced antagonistic action; Have the intestinal microflora of adjusting balance, safeguard functions such as intestinal health and enhance immunity power, be considered to the optimal antibiotic probiotic bacterium that substitutes.
In addition; Lactobacterium acidophilum has extremely strong acidproof, salt tolerant and anti-bile ability, can be in acidity that other milk-acid bacteria can not grow, hypersaline environment growth and breeding, can utilize glucose, fructose, lactose, sucrose to carry out homofermentation; Except that producing DL type lactic acid; Also comprise bacteriocin, VITAMINs, little peptide etc. in the fermentating metabolism thing, can improve the utilization ratio of calcium, phosphorus, iron, promote the absorption of iron and vitamins D; Reduce absorption of cholesterol, have the effect that growth of animal was digested and assimilated, promoted in enhancing.
Lactobacterium acidophilum has complicated nutritional requirement, difficult cultivation.At present the fermention medium of the domestic and international Lactobacterium acidophilum of reporting is mainly milk and adds glucose, peptone, yeast extract etc. or add other materials by glucose, peptone, beef extract, YE fully and forms, keeps the anaerobic state cultivation.With common substratum, as only adding glucose or sucrose with milk, culture effect is relatively poor, and the bacterium number is less; And starting material such as peptone, beef extract, YE are expensive, produce the Lactobacterium acidophilum cost in this way generally than higher.
Three, summary of the invention
The object of the invention is in order to overcome the high shortcoming of fermentation small scale, cost that traditional Lactobacterium acidophilum cultural method exists, the large-scaled culture method of a kind of Lactobacterium acidophilum to be provided.
The object of the invention is mainly realized through following technical scheme:
The large-scaled culture method of a kind of Lactobacterium acidophilum is characterized in that comprising the steps:
1) get bacterial classification ready, this bacterial classification is made up of aspergillus niger spore and Lactobacterium acidophilum, and the part by weight of aspergillus niger spore, Lactobacterium acidophilum is 8～9: 1; With aspergillus niger spore and Lactobacterium acidophilum preserve separately;
2) get substratum ready; This substratum is made up of rice bran, dregs of beans, NaCl and wheat bran; The quality percentage composition of each material component in substratum is rice bran 30～35%, dregs of beans 15～20%, NaCl 0.8～1% and wheat bran 44～54.2%, stirs after each material component is mixed;
3) solid fermentation; Substratum is poured in the solid-state fermentation tank; Press substratum 0.5～1 ‰ part by weight and insert aspergillus niger spore, add entry by substratum 60%～70% part by weight, relative humidity remains on 50%～60%; Uninterruptedly infeed filtrated air, per hour ventilation volume is 50%～60% of a fermentor tank volume; Ferment after 24 hours, press substratum 1% part by weight and insert Lactobacterium acidophilum, keep relative humidity and air demand constant, cultivated again 48 hours;
4) product packing is shifted out culture from fermentor tank, handled 10 hours through 50 ℃ of fluidised bed dryings, pulverizes the back packing again and obtains finished product.
The object of the invention can also reach through taking following technical scheme:
A kind of Technological improvement plan of realizing the object of the invention is: in the step 1), the preferable content of aspergillus niger spore can be 1 * 109/gram for the preferable content of 1 * 1010/gram, Lactobacterium acidophilum.
A kind of Technological improvement plan of realizing the object of the invention is: step 2) described substratum; The quality percentage composition of the material component of this substratum in substratum can be rice bran 32%, dregs of beans 17%, NaCl 0.9% and wheat bran 50.1%, stirs after each material component is mixed.
A kind of Technological improvement plan of realizing the object of the invention is: step 2) described substratum; The quality percentage composition of the material component of this substratum in substratum can be rice bran 30%, dregs of beans 20%, NaCl 0.8% and wheat bran 49.2%, stirs after each material component is mixed.
A kind of Technological improvement plan of realizing the object of the invention is: step 2) described substratum; The quality percentage composition of the material component of this substratum in substratum can be rice bran 35%, dregs of beans 15%, NaCl 1% and wheat bran 49%, stirs after each material component is mixed;
A kind of Technological improvement plan of realizing the object of the invention is: step 2) described substratum; The quality percentage composition of the material component of this substratum in substratum can be rice bran 30%, dregs of beans 15%, NaCl 0.8% and wheat bran 54.2%, stirs after each material component is mixed.
A kind of Technological improvement plan of realizing the object of the invention is: step 2) described substratum; The quality percentage composition of the material component of this substratum in substratum can be rice bran 30%, dregs of beans 15%, NaCl 0.8% and wheat bran 54.2%, stirs after each material component is mixed.
The present invention has following beneficial effect:
1, the present invention is owing to taked the prescription through the specific proportioning of applicant's TE and checking; Therefore; Comparatively the ideal mass-producing is cultivated, and has realized commercial solid fermentation, realizes low-cost, mass-producing cultivation; Have the beneficial effect that cost is low, production efficiency is high, can meet the need of market greatly.
2, the product that adopts the method for the invention to cultivate out can be used as the livestock and poultry animal fodder additives, has effects such as the animal feed intake of raising, conditioning enteron aisle, prevention diarrhoea, quickening growth.
Embodiment
Specific embodiment 1
The large-scaled culture method of present embodiment 1 said Lactobacterium acidophilum comprises the steps:
1) get bacterial classification ready, this bacterial classification is made up of aspergillus niger spore and Lactobacterium acidophilum, and the part by weight of aspergillus niger spore, Lactobacterium acidophilum is 8～9: 1; With aspergillus niger spore and Lactobacterium acidophilum preserve separately;
2) get substratum ready, this substratum is made up of rice bran, dregs of beans, NaCl and wheat bran, and the quality percentage composition of each material component in substratum is rice bran 32%, dregs of beans 17%, NaCl 0.9% and wheat bran 50.1%, stirs after each material component is mixed;
3) solid fermentation; Substratum is poured in the solid-state fermentation tank; Press substratum 0.5～1 ‰ part by weight and insert aspergillus niger spore, add entry by substratum 60%～70% part by weight, relative humidity remains on 50%～60%; Uninterruptedly infeed filtrated air, per hour ventilation volume is 50%～60% of a fermentor tank volume; Ferment after 24 hours, press substratum 1% part by weight and insert Lactobacterium acidophilum, keep relative humidity and air demand constant, cultivated again 48 hours;
4) product packing is shifted out culture from fermentor tank, handled 10 hours through 50 ℃ of fluidised bed dryings, pulverizes the back packing again and obtains finished product.
In the present embodiment, in the step 1), the preferable content of aspergillus niger spore can be 1 * 109/gram for the preferable content of 1 * 1010/gram, Lactobacterium acidophilum.
The effect of present embodiment:
20 kilograms of piglet application results are shown, in feed, add 2 ‰ products, used continuously 1 month, compare with control group, the speed of growth improves 12.7%, and feed coefficient descends 7.9%, and the diarrhoea incidence descends 61.3%.
Specific embodiment 2:
The characteristics of present embodiment 2 are: step 2) described substratum, the quality percentage composition of the material component of this substratum in substratum can be rice bran 30%, dregs of beans 20%, NaCl 0.8% and wheat bran 49.2%, and each material component is stirred after mixed.All the other method stepss are with specific embodiment 1.
Specific embodiment 3:
The characteristics of present embodiment 3 are: step 2) described substratum, the quality percentage composition of the material component of this substratum in substratum can be rice bran 35%, dregs of beans 15%, NaCl 1% and wheat bran 49%, and each material component is stirred after mixed.All the other method stepss are with specific embodiment 1.
Specific embodiment 4:
The characteristics of present embodiment 4 are: step 2) described substratum, the quality percentage composition of the material component of this substratum in substratum can be rice bran 30%, dregs of beans 15%, NaCl 0.8% and wheat bran 54.2%, and each material component is stirred after mixed.All the other method stepss are with specific embodiment 1.
Specific embodiment 5:
The characteristics of present embodiment 5 are: step 2) described substratum, the quality percentage composition of the material component of this substratum in substratum can be rice bran 30%, dregs of beans 15%, NaCl 0.8% and wheat bran 54.2%, and each material component is stirred after mixed.All the other method stepss are with specific embodiment 1.
Claims (7)
1. the large-scaled culture method of a Lactobacterium acidophilum is characterized in that:
1) get bacterial classification ready, this bacterial classification is made up of aspergillus niger spore and Lactobacterium acidophilum, and the part by weight of aspergillus niger spore, Lactobacterium acidophilum is 8～9: 1; With aspergillus niger spore and Lactobacterium acidophilum preserve separately;
2) get substratum ready; This substratum is made up of rice bran, dregs of beans, NaCl and wheat bran; The quality percentage composition of each material component in substratum is rice bran 30～35%, dregs of beans 15～20%, NaCl 0.8～1% and wheat bran 44～54.2%, stirs after each material component is mixed;
3) solid fermentation; Substratum is poured in the solid-state fermentation tank; Press substratum 0.5～1 ‰ part by weight and insert aspergillus niger spore, add entry by substratum 60%～70% part by weight, relative humidity remains on 50%～60%; Uninterruptedly infeed filtrated air, per hour ventilation volume is 50%～60% of a fermentor tank volume; Ferment after 24 hours, press substratum 1% part by weight and insert Lactobacterium acidophilum, keep relative humidity and air demand constant, cultivated again 48 hours;
4) product packing is shifted out culture from fermentor tank, handled 10 hours through 50 ℃ of fluidised bed dryings, pulverizes the back packing again and obtains finished product.
2. the large-scaled culture method of a kind of Lactobacterium acidophilum according to claim 1 is characterized in that: in the step 1), the preferable content of aspergillus niger spore is that the preferable content of 1 * 1010/gram, Lactobacterium acidophilum is 1 * 109/gram.
3. the large-scaled culture method of a kind of Lactobacterium acidophilum according to claim 1 and 2; It is characterized in that: step 2) described substratum; The quality percentage composition of the material component of this substratum in substratum is rice bran 32%, dregs of beans 17%, NaCl 0.9% and wheat bran 50.1%, stirs after each material component is mixed.
4. the large-scaled culture method of a kind of Lactobacterium acidophilum according to claim 1 and 2; It is characterized in that: step 2) described substratum; The quality percentage composition of the material component of this substratum in substratum is rice bran 30%, dregs of beans 20%, NaCl 0.8% and wheat bran 49.2%, stirs after each material component is mixed.
5. the large-scaled culture method of a kind of Lactobacterium acidophilum according to claim 1 and 2; It is characterized in that: step 2) described substratum; The quality percentage composition of the material component of this substratum in substratum is rice bran 35%, dregs of beans 15%, NaCl 1% and wheat bran 49%, stirs after each material component is mixed.
6. the large-scaled culture method of a kind of Lactobacterium acidophilum according to claim 1 and 2; It is characterized in that: step 2) described substratum; The quality percentage composition of the material component of this substratum in substratum is rice bran 30%, dregs of beans 15%, NaCl 0.8% and wheat bran 54.2%, stirs after each material component is mixed.
7. the large-scaled culture method of a kind of Lactobacterium acidophilum according to claim 1 and 2; It is characterized in that: step 2) described substratum; The quality percentage composition of the material component of this substratum in substratum is rice bran 30%, dregs of beans 15%, NaCl 0.8% and wheat bran 54.2%, stirs after each material component is mixed.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555611A (en) * | 2013-09-27 | 2014-02-05 | 上海源耀生物股份有限公司 | Lactobacillus acidophilus culture feed additive and preparation method thereof |
| CN106046126A (en) * | 2016-06-08 | 2016-10-26 | 江苏盐城源耀生物科技有限公司 | Broad-spectrum antimicrobial peptide produced by lactobacillus acidophilus and application of broad-spectrum antimicrobial peptide |
| CN108467846A (en) * | 2018-07-04 | 2018-08-31 | 中科宜康（北京）生物科技有限公司 | The cultural method of lactobacillus acidophilus and its application |
| CN108770993A (en) * | 2018-05-18 | 2018-11-09 | 广西开田三农科技有限责任公司 | A kind of preparation method of polypeptide feed |
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| CN101248845A (en) * | 2008-03-28 | 2008-08-27 | 成都枫澜科技有限公司 | Method for processing raw material of aquatic product feed stuff by fermenting |
| CN101664110A (en) * | 2009-09-28 | 2010-03-10 | 武汉多宝生物技术有限公司 | Animal feed additive and preparation process thereof |
| CN101912040A (en) * | 2010-09-08 | 2010-12-15 | 新疆天物科技发展有限公司 | Tomato pomace biological fermentation feed and preparation method thereof |
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2012
- 2012-03-31 CN CN2012100930904A patent/CN102618470A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101248845A (en) * | 2008-03-28 | 2008-08-27 | 成都枫澜科技有限公司 | Method for processing raw material of aquatic product feed stuff by fermenting |
| CN101664110A (en) * | 2009-09-28 | 2010-03-10 | 武汉多宝生物技术有限公司 | Animal feed additive and preparation process thereof |
| CN101912040A (en) * | 2010-09-08 | 2010-12-15 | 新疆天物科技发展有限公司 | Tomato pomace biological fermentation feed and preparation method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555611A (en) * | 2013-09-27 | 2014-02-05 | 上海源耀生物股份有限公司 | Lactobacillus acidophilus culture feed additive and preparation method thereof |
| CN106046126A (en) * | 2016-06-08 | 2016-10-26 | 江苏盐城源耀生物科技有限公司 | Broad-spectrum antimicrobial peptide produced by lactobacillus acidophilus and application of broad-spectrum antimicrobial peptide |
| CN108770993A (en) * | 2018-05-18 | 2018-11-09 | 广西开田三农科技有限责任公司 | A kind of preparation method of polypeptide feed |
| CN108467846A (en) * | 2018-07-04 | 2018-08-31 | 中科宜康（北京）生物科技有限公司 | The cultural method of lactobacillus acidophilus and its application |
| CN108467846B (en) * | 2018-07-04 | 2019-02-15 | 中科宜康（北京）生物科技有限公司 | Probiotics solid beverage |
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Application publication date: 20120801 |