CN106046126A - Broad-spectrum antimicrobial peptide produced by lactobacillus acidophilus and application of broad-spectrum antimicrobial peptide - Google Patents
Broad-spectrum antimicrobial peptide produced by lactobacillus acidophilus and application of broad-spectrum antimicrobial peptide Download PDFInfo
- Publication number
- CN106046126A CN106046126A CN201610403172.2A CN201610403172A CN106046126A CN 106046126 A CN106046126 A CN 106046126A CN 201610403172 A CN201610403172 A CN 201610403172A CN 106046126 A CN106046126 A CN 106046126A
- Authority
- CN
- China
- Prior art keywords
- antimicrobial peptide
- spectrum antimicrobial
- broad
- broad spectrum
- bacillus acidophilus
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/335—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Lactobacillus (G)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Abstract
The invention discloses broad-spectrum antimicrobial peptide produced by lactobacillus acidophilus and an application of the broad-spectrum antimicrobial peptide. The broad-spectrum antimicrobial peptide is prepared though the following steps: step S1, fermentation solids of the lactobacillus acidophilus is mixed and stirred with water in a mass volume ratio of 1:(2-4) and a mixture is left to stand; step S2, supernatant after standing of the mixture is centrifuged, and centrifugal supernatant is collected; step S3, the centrifugal supernatant is treated with an ultrafiltration membrane to retain components with molecular weight lower than 10 kDa; step S4, the components with molecular weight lower than 10 kDa is ultra-filtered continuously, and components with molecular weight ranging from 6 kDa to 8 kDa are retained, concentrated and freeze-dried, and the broad-spectrum antimicrobial peptide is prepared. The broad-spectrum antimicrobial peptide produced by the lactobacillus acidophilus has stronger inhibitory effect on bacteria and higher thermal stability, and has prominent substantive features and notable progress compared with the prior art.
Description
Technical field
The present invention relates to a kind of broad spectrum antimicrobial peptide produced by bacillus acidophilus and application thereof.
Background technology
Conventional antibiotic achieves remarkable result in terms for the treatment of bacterial infection disease, but in recent years, along with tradition
Antibiotic is at the abuse in the field such as medical science and aquaculture, and microorganism creates the strongest toleration to conventional antibiotic, with
Time also occur in that the superbacteria that some drug resistance are extremely strong, microorganism resistance problems have become the difficulty of serious threat human health
Topic.So being badly in need of developing the antimicrobial agents of a kind of new safety.
Antibacterial peptide is a kind of micromolecule polypeptide, and antibacterial, fungus, virus and protozoon etc. are respectively provided with killing action.Antibacterial peptide
There is the features such as molecular weight is little, simple in construction, bactericidal activity are strong.It can kill enteric pathogenic bacteria and regulating intestinal canal is healthy, and
Preferably hydrolyzed by protease (trypsin), will not remain in body.Therefore antibacterial peptide is expected to become a kind of novel efficient
Antibacterials, have wide development prospect.
Summary of the invention
It is an object of the invention to provide a kind of broad spectrum antimicrobial peptide produced by bacillus acidophilus and application thereof.
The above-mentioned purpose of the present invention is achieved by techniques below scheme:
A kind of broad spectrum antimicrobial peptide produced by bacillus acidophilus, is made by the steps and forms:
Step S1, by bacillus acidophilus's solid fermentation thing with water according to mass volume ratio 1:2～4 mix and blends, stands；
Step S2, takes the supernatant after said mixture stands, centrifugal, collects centrifuged supernatant；
Step S3, crosses ultrafilter membrane by centrifuged supernatant, and molecular cut off is not more than the component of 10kDa；
Step S4, above-mentioned molecular weight is not more than 10kDa component continue ultrafiltration, the component of molecular cut off 6～8kDa,
Concentrating, lyophilization i.e. obtains described broad spectrum antimicrobial peptide.
Further, described bacillus acidophilus's solid fermentation thing be in bean cake, Semen Maydis, wheat bran one or more with addicted to acid
Through solid fermentation products therefrom after lactobacillus mixing.
Further, in step S2, the described centrifugal 5000rpm that is specially is centrifuged 10min.
Above-mentioned broad spectrum antimicrobial peptide is as the purposes of feed additive.
Above-mentioned broad spectrum antimicrobial peptide is for preparing the purposes of the medicine of suppression antibacterial.
Advantages of the present invention:
The broad spectrum antimicrobial peptide produced by bacillus acidophilus that the present invention provides is higher and heat stability to the inhibition of antibacterial
Higher, compared with prior art there is prominent substantive distinguishing features and significantly progress.
Detailed description of the invention
Further illustrate the essentiality content of the present invention below in conjunction with embodiment, but do not limit the present invention with this and protect model
Enclose.Although the present invention being explained in detail with reference to preferred embodiment, it will be understood by those within the art that, can be right
Technical scheme is modified or equivalent, without deviating from the spirit and scope of technical solution of the present invention.
In following embodiment, described bacillus acidophilus's solid fermentation thing be in bean cake, Semen Maydis, wheat bran one or more with
Through solid fermentation products therefrom after bacillus acidophilus's mixing.
Embodiment 1: the preparation of the broad spectrum antimicrobial peptide produced by bacillus acidophilus
Step S1, by bacillus acidophilus's solid fermentation thing with water according to mass volume ratio 1:3 mix and blend, stands；
Step S2, takes the supernatant after said mixture stands, and 5000rpm is centrifuged 10min, collects centrifuged supernatant；
Step S3, crosses ultrafilter membrane by centrifuged supernatant, and molecular cut off is not more than the component of 10kDa；
Step S4, above-mentioned molecular weight is not more than 10kDa component continue ultrafiltration, the component of molecular cut off 6～8kDa,
Concentrating, lyophilization i.e. obtains described broad spectrum antimicrobial peptide.
Embodiment 2: comparative example, the component of molecular cut off 5～6kDa
Step S1, by bacillus acidophilus's solid fermentation thing with water according to mass volume ratio 1:3 mix and blend, stands；
Step S2, takes the supernatant after said mixture stands, and 5000rpm is centrifuged 10min, collects centrifuged supernatant；
Step S3, crosses ultrafilter membrane by centrifuged supernatant, and molecular cut off is not more than the component of 10kDa；
Step S4, above-mentioned molecular weight is not more than 10kDa component continue ultrafiltration, the component of molecular cut off 5～6kDa,
Concentrate, lyophilization.
Embodiment 3: comparative example, the component of molecular cut off 8～9kDa
Step S1, by bacillus acidophilus's solid fermentation thing with water according to mass volume ratio 1:3 mix and blend, stands；
Step S2, takes the supernatant after said mixture stands, and 5000rpm is centrifuged 10min, collects centrifuged supernatant；
Step S3, crosses ultrafilter membrane by centrifuged supernatant, and molecular cut off is not more than the component of 10kDa；
Step S4, above-mentioned molecular weight is not more than 10kDa component continue ultrafiltration, the component of molecular cut off 8～9kDa,
Concentrate, lyophilization.
Embodiment 4: effect example
Respectively antibacterial peptide prepared by embodiment 1～3 is configured to the test liquid 1～3 that concentration is 1mg/100ml.Survey respectively
Examination test liquid 1～the 3 pairs of escherichia coli, Salmonella, staphylococcus aureus and Listerella suppression ratio (%)；Again by test liquid
1～3 under the conditions of 121 DEG C heat treatment 30min, test heat treatment after to escherichia coli, Salmonella, staphylococcus aureus and
Listerella suppression ratio (%).
Experimental result see table.
The above results shows, the broad spectrum antimicrobial peptide produced by bacillus acidophilus that the present invention provides is to the inhibition of antibacterial more
Strong and heat stability is higher, compared with prior art there is prominent substantive distinguishing features and the most progressive.
The effect of above-described embodiment indicates that the essentiality content of the present invention, but does not limit the protection of the present invention with this
Scope.It will be understood by those within the art that, technical scheme can be modified or equivalent,
Essence and protection domain without deviating from technical solution of the present invention.
Claims (5)
1. the broad spectrum antimicrobial peptide produced by bacillus acidophilus, it is characterised in that be made by the steps and form:
Step S1, by bacillus acidophilus's solid fermentation thing with water according to mass volume ratio 1:2～4 mix and blends, stands；
Step S2, takes the supernatant after said mixture stands, centrifugal, collects centrifuged supernatant；
Step S3, crosses ultrafilter membrane by centrifuged supernatant, and molecular cut off is not more than the component of 10kDa；
Step S4, above-mentioned molecular weight is not more than 10kDa component continue ultrafiltration, the component of molecular cut off 6～8kDa, dense
Contracting, lyophilization i.e. obtains described broad spectrum antimicrobial peptide.
Broad spectrum antimicrobial peptide the most according to claim 1, it is characterised in that: described bacillus acidophilus's solid fermentation thing is bean
One or more in the dregs of rice, Semen Maydis, wheat bran mix with bacillus acidophilus after through solid fermentation products therefrom.
Broad spectrum antimicrobial peptide the most according to claim 1, it is characterised in that: in step S2, described centrifugal specially 5000rpm
Centrifugal 10min.
4. the arbitrary described broad spectrum antimicrobial peptide of claims 1 to 3 is as the purposes of feed additive.
5. the arbitrary described broad spectrum antimicrobial peptide of claims 1 to 3 is for preparing the purposes of the medicine of suppression antibacterial.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610403172.2A CN106046126A (en) | 2016-06-08 | 2016-06-08 | Broad-spectrum antimicrobial peptide produced by lactobacillus acidophilus and application of broad-spectrum antimicrobial peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610403172.2A CN106046126A (en) | 2016-06-08 | 2016-06-08 | Broad-spectrum antimicrobial peptide produced by lactobacillus acidophilus and application of broad-spectrum antimicrobial peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106046126A true CN106046126A (en) | 2016-10-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610403172.2A Pending CN106046126A (en) | 2016-06-08 | 2016-06-08 | Broad-spectrum antimicrobial peptide produced by lactobacillus acidophilus and application of broad-spectrum antimicrobial peptide |
Country Status (1)
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102618470A (en) * | 2012-03-31 | 2012-08-01 | 广东绿百多生物科技有限公司 | Scale-up culture method for lactobacillus acidophilus |
| CN105566453A (en) * | 2016-01-25 | 2016-05-11 | 江苏盐城源耀生物科技有限公司 | Separation and purification method for lactococcus acidophilus antibacterial peptide |
-
2016
- 2016-06-08 CN CN201610403172.2A patent/CN106046126A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102618470A (en) * | 2012-03-31 | 2012-08-01 | 广东绿百多生物科技有限公司 | Scale-up culture method for lactobacillus acidophilus |
| CN105566453A (en) * | 2016-01-25 | 2016-05-11 | 江苏盐城源耀生物科技有限公司 | Separation and purification method for lactococcus acidophilus antibacterial peptide |
Non-Patent Citations (2)
| Title |
|---|
| M.ZAMFIR ET AL.: "Purification and characterization of a bacteriocin produced by Lactobacillus acidophilus IBB 801", 《JOURNAL OF APPLIED MICROBIOLOGY》 * |
| SAHAR F.DERAZ ET AL.: "Purification and characterisation of acidocin D20079, a bacteriocin produced by Lactobacillus acidophilus DSM 20079", 《JOURNAL OF BIOTECHNOLOGY》 * |
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| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161026 |
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| RJ01 | Rejection of invention patent application after publication |