CN102612554A - 用于细胞培养的微流控设备 - Google Patents

用于细胞培养的微流控设备 Download PDF

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Publication number
CN102612554A
CN102612554A CN2010800468967A CN201080046896A CN102612554A CN 102612554 A CN102612554 A CN 102612554A CN 2010800468967 A CN2010800468967 A CN 2010800468967A CN 201080046896 A CN201080046896 A CN 201080046896A CN 102612554 A CN102612554 A CN 102612554A
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cells
cell
chamber
cell culture
perfusion
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Chinese (zh)
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R·A·法利斯
V·N·戈拉尔
谢宜铮
O·N·佩措尔德
阮宝祺
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Corning Inc
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Corning Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/40—Manifolds; Distribution pieces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Dispersion Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN2010800468967A 2009-10-12 2010-10-12 用于细胞培养的微流控设备 Pending CN102612554A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25075409P 2009-10-12 2009-10-12
US61/250,754 2009-10-12
PCT/US2010/052212 WO2011046884A2 (en) 2009-10-12 2010-10-12 Microfluidic device for cell culture

Publications (1)

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CN102612554A true CN102612554A (zh) 2012-07-25

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US (2) US8481303B2 (enExample)
EP (1) EP2488628A2 (enExample)
JP (1) JP2013507922A (enExample)
CN (1) CN102612554A (enExample)
WO (1) WO2011046884A2 (enExample)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104293666A (zh) * 2014-09-11 2015-01-21 大连理工大学 两种不同单细胞之间相互作用的微流控芯片装置
CN105229466A (zh) * 2013-03-15 2016-01-06 克里斯蒂娜·D·林克 具有端口的平行板流动室及其使用方法
CN109310502A (zh) * 2016-02-16 2019-02-05 马特里优米克斯有限公司 用于改变活细胞生理机能的表面形貌
CN111068516A (zh) * 2020-01-18 2020-04-28 浙江大学 多元强化耦合智能调控防止高粘性灰在催化剂表面沉积的系统及方法
CN113825831A (zh) * 2019-05-13 2021-12-21 纽索思创新有限公司 用于细胞培养的微流控装置和方法
CN114453039A (zh) * 2022-02-08 2022-05-10 浙江扬清芯片技术有限公司 一种离心式微流控芯片点阵阀结构

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7534601B2 (en) * 2002-08-27 2009-05-19 Vanderbilt University Capillary perfused bioreactors with multiple chambers
JP2012509663A (ja) * 2008-11-21 2012-04-26 コーニング インコーポレイテッド 細胞培養のための、突起間隔を開けた基板および装置
AU2010319881A1 (en) * 2009-10-29 2012-05-03 The Charles Stark Draper Laboratory, Inc. Microfluidic device for blood dialysis
US9522396B2 (en) 2010-12-29 2016-12-20 S.D. Sight Diagnostics Ltd. Apparatus and method for automatic detection of pathogens
CN102234614B (zh) * 2011-05-30 2013-02-13 中国科学院苏州纳米技术与纳米仿生研究所 微流控细胞悬浮培养芯片及其应用
EP2714892B1 (en) 2011-06-02 2018-02-21 President and Fellows of Harvard College Methods and uses for ex vivo tissue culture systems
CA2839435C (en) * 2011-06-15 2021-05-11 The Charles Stark Draper Laboratory, Inc. Systems, methods, and devices relating to a biomimetic cellularized nephron unit
JP2014516582A (ja) * 2011-06-15 2014-07-17 ザ チャールズ スターク ドレイパー ラボラトリー インク 生体模倣環境において細胞を培養するためのデバイスおよび方法
WO2013098821A1 (en) 2011-12-29 2013-07-04 Parasight Ltd. Methods and systems for detecting a pathogen in a biological sample
WO2014031523A2 (en) * 2012-08-19 2014-02-27 University Of Rochester Method for preparing microfluidic device with reduced channel height
US20140127733A1 (en) * 2012-11-01 2014-05-08 Moffit Cancer Center Ex vivo microfluidic analysis of biologic samples
EP3869257B1 (en) 2013-05-23 2024-05-08 S.D. Sight Diagnostics Ltd. Method and system for imaging a cell sample
IL227276A0 (en) 2013-07-01 2014-03-06 Parasight Ltd A method and system for preparing a monolayer of cells, particularly suitable for diagnosis
EP3024921B1 (en) 2013-07-22 2025-12-10 The Regents of The University of California Microfluidics cell culture device
EP3955042B1 (en) 2013-08-26 2024-08-21 S.D. Sight Diagnostics Ltd. Digital microscopy systems, methods and computer program products
US20150064689A1 (en) * 2013-08-29 2015-03-05 The Charles Stark Draper Laboratory, Inc. System and method for analyzing transmissibility of influenza
CN103627635B (zh) * 2013-11-18 2015-05-20 辽宁中医药大学 一种用于细胞迁移和侵袭实验的多功能微流控芯片
US20150247112A1 (en) * 2014-03-03 2015-09-03 Kiyatec Inc. 3D Tissue Culture Devices and Systems
US9631167B2 (en) 2014-03-21 2017-04-25 Iontox, Llc Dynamic multi organ plate
EP3177709B1 (en) * 2014-08-07 2022-05-18 The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone Reversible stencils for fabricating micro-tissues
US10482595B2 (en) 2014-08-27 2019-11-19 S.D. Sight Diagnostics Ltd. System and method for calculating focus variation for a digital microscope
CN105713835B (zh) * 2014-12-05 2018-11-09 中国科学院大连化学物理研究所 一种基于微流控芯片的多功能区域细胞三维共培养方法
US10279345B2 (en) 2014-12-12 2019-05-07 Opko Diagnostics, Llc Fluidic systems comprising an incubation channel, including fluidic systems formed by molding
AU2016250689B2 (en) 2015-04-22 2021-07-08 Berkeley Lights, Inc. Microfluidic cell culture
GB201512600D0 (en) * 2015-07-17 2015-08-26 Koniku Ltd Cell culture, transport and investigation
AU2016322966B2 (en) 2015-09-17 2021-10-14 S.D. Sight Diagnostics Ltd Methods and apparatus for detecting an entity in a bodily sample
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
WO2017096297A1 (en) 2015-12-04 2017-06-08 EMULATE, Inc. Open-top microfluidic device with structural anchors
EP3436035B1 (en) * 2016-03-29 2024-09-04 SmsBiotech, Inc. Compositions and methods for using small mobile stem cells
WO2017168411A1 (en) 2016-03-30 2017-10-05 S.D. Sight Diagnostics Ltd Image processing device for identifying blood parasites
WO2017184595A1 (en) * 2016-04-18 2017-10-26 Saint Louis University Integration of three-dimensional cell culture scaffolds in microfluidic devices by direct fiber spinning
US11307196B2 (en) 2016-05-11 2022-04-19 S.D. Sight Diagnostics Ltd. Sample carrier for optical measurements
CN109564209B (zh) 2016-05-11 2022-05-31 思迪赛特诊断有限公司 对样品实施的光学测量
AU2017271673B2 (en) 2016-05-26 2022-04-14 Berkeley Lights, Inc. Covalently modified surfaces, kits, and methods of preparation and use
CN105838606B (zh) * 2016-06-08 2018-10-02 牛海涛 一种高通量细胞联合培养芯片
US10243097B2 (en) * 2016-09-09 2019-03-26 eLux Inc. Fluidic assembly using tunable suspension flow
WO2018080308A2 (en) * 2016-10-28 2018-05-03 Materiomics B.V. Surface topographies for stimulating endothelialization
WO2018208332A2 (en) 2017-02-17 2018-11-15 Koniku, Inc. Systems for detection
US11572590B2 (en) 2017-03-31 2023-02-07 Dana-Farber Cancer Institute, Inc. Methods for evaluating tumor cell spheroids using 3D microfluidic cell culture device
WO2018187181A1 (en) 2017-04-03 2018-10-11 The Charles Stark Draper Laboratory, Inc. Microfluidic system for evaluation of chemotherapeutic and immunotherapeutic drugs
EP3710810B1 (en) 2017-11-14 2023-09-06 S.D. Sight Diagnostics Ltd. Sample carrier for optical measurements
KR101979145B1 (ko) * 2018-01-30 2019-05-15 서강대학교산학협력단 신경줄기세포 배양용 미세유체칩 및 이의 용도
WO2020081288A1 (en) * 2018-10-14 2020-04-23 Arizona Board Of Regents On Behalf Of Arizona State University Microfluidic devices and methods incorporating organized three-dimensional tissue constructs
KR102170851B1 (ko) * 2018-11-13 2020-10-27 엠비디 주식회사 세포 배양 플레이트
JP7677972B2 (ja) 2019-12-12 2025-05-15 エス．ディー．サイト ダイアグノスティックス リミテッド カラー血液スミア画像の人工的な生成
AU2020400401A1 (en) 2019-12-12 2022-06-02 S.D. Sight Diagnostics Ltd Analyzing an analyte disposed within a medium
JP7421763B2 (ja) * 2020-12-02 2024-01-25 ウシオ電機株式会社 培養容器及び培養容器の使用方法
WO2022159097A1 (en) * 2021-01-22 2022-07-28 Hewlett-Packard Development Company, L.P. Microfluidic device chamber pillars
EP4363606A4 (en) * 2021-06-30 2024-08-21 Hewlett-Packard Development Company, L.P. CHAMBER COLUMNS OF A MICROFLUIDIC DEVICE
US12186748B2 (en) 2022-06-24 2025-01-07 Hewlett-Packard Development Company, L.P. Self-priming microfluidic structures
CN115742293A (zh) * 2022-11-01 2023-03-07 福建星海通信科技有限公司 一种基于dlp3d打印的生物应用微流体器件制备方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6653124B1 (en) * 2000-11-10 2003-11-25 Cytoplex Biosciences Inc. Array-based microenvironment for cell culturing, cell monitoring and drug-target validation
WO2006052223A1 (en) * 2004-11-11 2006-05-18 Agency For Science, Technology And Research Cell culture device

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1437404A (en) 1973-09-24 1976-05-26 Kaye S Method and means for assaying biological factors demonstrating quantal response
EP0075973B1 (de) 1981-09-18 1987-01-21 BBC Brown Boveri AG Gleichdruckluftspeicherkaverne mit hydraulischem Druckausgleich für Luftspeichergasturbinenkraftwerke
DE4116727C2 (de) 1991-05-17 1995-09-07 Uwe Dr Marx Verfahren und Vorrichtung zur gleichzeitigen Kultivierung unterschiedlicher Säugerzellen
DE4132379A1 (de) 1991-09-28 1993-04-08 Kernforschungsz Karlsruhe Substrat fuer zellkulturen und kultur von zellen oder zellaggregaten
DE69433550T2 (de) 1993-11-02 2009-09-10 Bio-Lok International, Inc., Deerfield Beach Zellenwuchskontrolle
FR2741357B1 (fr) 1995-11-22 1998-01-16 Corning Inc Procede de fabrication d'une plaquette de support d'un reseau bidimensionnel de micropuits, notamment pour essais ou cultures biologiques
US6391578B2 (en) 1997-04-09 2002-05-21 3M Innovative Properties Company Method and devices for partitioning biological sample liquids into microvolumes
CH692583A5 (de) 1998-03-03 2002-08-15 Weidmann H Ag Kulturgefäss.
GB9805214D0 (en) 1998-03-11 1998-05-06 Univ Glasgow Cell adhesion
ATE227338T1 (de) 1998-03-18 2002-11-15 Massachusetts Inst Technology Vaskularisierte perfundierte anordnungen für mikrogewebe und mikroorgane
JP2000231007A (ja) 1999-02-09 2000-08-22 Ricoh Opt Ind Co Ltd 凹型微細形状のアレイ状パターン形成方法及びその形成方法を用いて製作される平板型レンズアレイ及び液晶表示素子及び平板型オイルトラップ
US7033821B2 (en) 2000-11-08 2006-04-25 Surface Logix, Inc. Device for monitoring cell motility in real-time
US7211209B2 (en) 2000-11-08 2007-05-01 Surface Logix, Inc. Method of making device for arraying biomolecules and for monitoring cell motility in real-time
US7018418B2 (en) 2001-01-25 2006-03-28 Tecomet, Inc. Textured surface having undercut micro recesses in a surface
US7288405B2 (en) 2001-04-25 2007-10-30 Cornell Research Foundation, Inc. Devices and methods for pharmacokinetic-based cell culture system
ATE406436T1 (de) 2001-07-26 2008-09-15 Transparent Inc Konstrukt aus kultivierten zellen mit spheroiden von kultivierten tierzellen und verwendung davon
US20030064000A1 (en) 2001-09-24 2003-04-03 Wilson Burgess Methods of sterilizing biological mixtures using stabilizer mixtures
WO2003101618A1 (en) 2002-05-31 2003-12-11 Cancer Research Technology Ltd Substrate for holding an array of experimental samples
US20040067585A1 (en) 2002-10-07 2004-04-08 Yu-Chi Wang Cell cultivation surface and method of making the same
US7190449B2 (en) 2002-10-28 2007-03-13 Nanopoint, Inc. Cell tray
DE60333715D1 (de) 2002-10-30 2010-09-23 Hitachi Ltd Verfahren zur Herstellung funktioneller Substrate, die kolumnare Mikrosäulen aufweisen
WO2004048064A1 (ja) 2002-11-27 2004-06-10 Japan Science And Technology Agency 微細突起構造体及びその製造方法
US7329537B2 (en) 2003-04-17 2008-02-12 Nexcelom Bioscience, Llc Micro-pattern embedded plastic optical film device for cell-based assays
CA2524496A1 (en) 2003-05-06 2004-11-25 Bellbrook Labs, Llc Three dimensional cell cultures in a microscale fluid handling system
EP1901067A3 (en) 2004-08-03 2009-05-13 On-Chip Cellomics Consortium Cellomics system
US20060228386A1 (en) 2005-02-22 2006-10-12 University Of Tennessee Research Foundation Polymeric microstructures
EP1875234B1 (en) 2005-04-26 2011-06-22 Aarhus Universitet Biosurface structure array
GB2427688A (en) 2005-06-28 2007-01-03 Inogen S A Microwell plate
KR20110086160A (ko) 2005-09-15 2011-07-27 스위트 파워 아이엔씨. 플렉시블 기판 및 마이크로 필러 구조를 구비하는 미생물 연료전지
US20070249044A1 (en) 2006-01-30 2007-10-25 University Of Illinois At Chicago Microstructures in three dimensional gel suspensions for growth of cells
JP4506987B2 (ja) 2006-07-21 2010-07-21 株式会社日立ハイテクノロジーズ エネルギ線硬化型樹脂の転写方法、転写装置及びディスクまたは半導体デバイス
JP5013584B2 (ja) 2006-08-30 2012-08-29 株式会社日立製作所 軟骨細胞の培養方法および軟骨細胞

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6653124B1 (en) * 2000-11-10 2003-11-25 Cytoplex Biosciences Inc. Array-based microenvironment for cell culturing, cell monitoring and drug-target validation
WO2006052223A1 (en) * 2004-11-11 2006-05-18 Agency For Science, Technology And Research Cell culture device
EP1815244A1 (en) * 2004-11-11 2007-08-08 Agency for Science, Technology and Research Cell culture device

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105229466A (zh) * 2013-03-15 2016-01-06 克里斯蒂娜·D·林克 具有端口的平行板流动室及其使用方法
CN105229466B (zh) * 2013-03-15 2018-05-29 克里斯蒂娜·D·林克 具有端口的平行板流动室及其使用方法
CN104293666A (zh) * 2014-09-11 2015-01-21 大连理工大学 两种不同单细胞之间相互作用的微流控芯片装置
CN104293666B (zh) * 2014-09-11 2016-06-22 大连理工大学 两种不同单细胞之间相互作用的微流控芯片装置
CN109310502A (zh) * 2016-02-16 2019-02-05 马特里优米克斯有限公司 用于改变活细胞生理机能的表面形貌
CN113825831A (zh) * 2019-05-13 2021-12-21 纽索思创新有限公司 用于细胞培养的微流控装置和方法
CN113825831B (zh) * 2019-05-13 2024-09-17 纽索思创新有限公司 用于细胞培养的微流控装置和方法
CN111068516A (zh) * 2020-01-18 2020-04-28 浙江大学 多元强化耦合智能调控防止高粘性灰在催化剂表面沉积的系统及方法
CN111068516B (zh) * 2020-01-18 2023-11-14 浙江大学 多元强化耦合智能调控防止高粘性灰在催化剂表面沉积的系统及方法
CN114453039A (zh) * 2022-02-08 2022-05-10 浙江扬清芯片技术有限公司 一种离心式微流控芯片点阵阀结构

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EP2488628A2 (en) 2012-08-22
WO2011046884A3 (en) 2012-01-26
US8481303B2 (en) 2013-07-09
US20130273589A1 (en) 2013-10-17
JP2013507922A (ja) 2013-03-07
US8679843B2 (en) 2014-03-25
US20110086427A1 (en) 2011-04-14
WO2011046884A2 (en) 2011-04-21

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