CN102608129B - Shimmer detecting system - Google Patents
Shimmer detecting system Download PDFInfo
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- CN102608129B CN102608129B CN201210071730.1A CN201210071730A CN102608129B CN 102608129 B CN102608129 B CN 102608129B CN 201210071730 A CN201210071730 A CN 201210071730A CN 102608129 B CN102608129 B CN 102608129B
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- reaction cup
- leaded light
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Abstract
The invention discloses a shimmer detecting system, which comprises a reaction cup (100), a compressing and sealing device (200), a light guiding device (300) and a light metering sensor (400), wherein the reaction cup (100) is set to be used for containing luminescent substances to be detected; the compressing and sealing device (200) is provided with a first connecting part and a second connecting part; the first connecting part is connected with the reaction cup (100), so that a darkroom is formed by the first connecting part and the reaction cup; the second connecting part is connected with the light guiding device (300), so that light rays of the luminescent substances are transmitted to the light metering sensor (400) from the light guiding device; and the light metering sensor is connected with the light guiding device, and is set to be used for detecting the light rays so as to detect the luminescent substances.
Description
Technical field
The present invention relates to a kind of micro light measuring system, it is for full-automatic food security detection and analysis equipment.
Background technology
At present, in food safety detection, the conventional sense method of agricultural and veterinary chemicals residual quantity mainly contains high performance liquid chromatography (HPLC), GC-MS (GC-MS), liquid chromatography-mass spectrography (LC-MS) and euzymelinked immunosorbent assay (ELISA) (ELISA).
1, high performance liquid chromatography (HPLC) has the advantages that accuracy of detection is high, false positive rate is low.The major defect of HPLC method is that instrument is expensive, operate more loaded down with trivial details, length consuming time, testing cost costliness, has relatively high expectations to testing staff's specialty, Sample pretreatment is more complicated.
2, gas chromatography-mass spectrography (GC-MS) sensitivity is very high, and false positive rate is low.The major defect of the method is that sample needs derivatization to process complicated like this early stage.Although the sample through derivatization treatment can provide more structural information in mass spectrum, derivatization can produce multiple different products and cause the partial loss of sample, causes the deviation of experimental result.
3, liquid chromatography-mass spectrography (LC-MS), sample does not need derivatization treatment, can detect urine, blood, liver, hair and eyeball sample.LC-MS/MS coupling, can further improve signal to noise ratio (S/N ratio), so can be used for the confirmation means to positive findings.But, no matter LC-MS or LC-MS/MS, instrument detection method is the same with GC-MS, and fails to solve instrument and involve great expense, complex operation step, Sample pretreatment complexity, requires the problems such as high to operating personnel's Specialized Quality.
4, euzymelinked immunosorbent assay (ELISA) (ELISA) starts to apply in China's food safety detection field the nineties, relies at present the kit of elisa technique to become the leading products in food security fast detecting field.The specific reaction of enzyme linked immunosorbent detection method based on Ag-Ab, detection sensitivity is higher, specificity is better, and technical operation is simple and easy, easily grasps.But there are many defects that self cannot overcome in ELISA method, be mainly manifested in human factor hand-manipulated easily cause detect error, sensing range is narrow, sensitivity is lower.
Chemiluminescent immunoassay (CLIA) is a kind of supersensitive microdetermination technology, the free energy that this technology utilizes chemical reaction to discharge excites intermediate, make it get back to ground state from excited state, the photon of energy level such as discharge, mensuration through chemical illumination immunity analysis instrument to photon, can accurate quantification measured object content.This technology is mainly used in the clinical detection such as infectious disease, tumor markers, hormonal readiness at present.
Applicant of the present invention introduces chemiluminescence method food safety detection field first, utilizes chemiluminescent detection principle to develop a full-automatic food safety detector.This instrument has with respect to chromatograph, mass spectrometer that Sample pretreatment is simple, full automatic working requires low, instrument low price feature to operating personnel's specialty, and detection sensitivity is suitable with LC-MS.This instrument has the advantages that with respect to microplate reader high sensitivity, detection time are short, artificial interference factor is exempted in full automatic working.Wherein, micro light measuring system is wherein most important parts of this instrument.
Micro light measuring system wherein most critical be to manufacture a black out darkroom, prevent the interference of ambient light.At present, on the Full-automatic chemiluminescence apparatus of applying on market, conventionally adopt special measurement darkroom for luminescent substance is measured.The present invention utilizes reaction cup and one of the photodetection self-forming measured with black to measure darkroom for photometry, has reduced turnover darkroom mechanism, has improved the operational efficiency of light-shading effect and machine.
Summary of the invention
For the aspect solving the problems of the technologies described above, the invention provides a kind of micro light measuring system, it comprises: reaction cup (100), pressed sealing device (200), guiding device (300) and photometry sensor (400), and described reaction cup (100) is arranged to hold luminescent substance to be detected; Described pressed sealing device (200) has the first connecting portion and the second connecting portion, and described the first connecting portion is connected with described reaction cup (100), and is arranged to make described the first connecting portion and described reaction cup to form darkroom; Described the second connecting portion is connected with described guiding device (300), so that the light of luminescent substance is transferred to described photometry sensor (400) from described guiding device; Described photometry sensor is connected with described guiding device, and described photometry sensor is arranged to detect described light, thereby detects described luminescent substance.
Preferably, described first connecting portion of described pressed sealing device (200) comprises caulking gum portion, and described caulking gum portion matches with described reaction cup (100).
Preferably, described reaction cup (100) comprises groove, and described caulking gum portion matches with described groove.
Preferably, described the second connecting portion comprises compact heap, leaded light sleeve and leaded light fixed block, described compact heap is arranged to around described leaded light sleeve, and both can vertically slidably connect, described guiding device is through described leaded light sleeve, described leaded light fixed block is arranged on one end of described leaded light sleeve, so that described guiding device is fixed, the other end of described leaded light sleeve is connected with described the first connecting portion.
Preferably, between described compact heap and described leaded light sleeve, spring is set, and described spring is arranged to, in the time that described the first connecting portion contact with described reaction cup, described leaded light sleeve freedom under the effect of the elastic force of described spring is sagging; In the time that described the first connecting portion contacts with described reaction cup, described spring produces snap-in force on described leaded light sleeve, thereby, make to produce snap-in force between described the first connecting portion and described reaction cup.
Preferably, described pressed sealing device (200) also comprises drive division, and described drive division drives described compact heap vertically to move up and down.
Preferably, described drive division comprises motor, slide block and leading screw, and described slide block is arranged to be connected with described leading screw, and be connected with described compact heap, described motor drives described leading screw to rotate, and described leading screw drives described slide block to slide up and down, thereby described compact heap slides up and down.
Preferably, described reaction cup is made up of black light-proof material.
Preferably, described guiding device (300) comprises leading shaven head, rear leaded light head and liquid-core optical fibre, and described leading shaven head is for being connected with the second connecting portion of described pressed sealing device; Described rear leaded light head is for being connected with described photometry sensor; Described liquid-core optical fibre is for transmitting the light that described luminescent substance sends.
Preferably, described photometry sensor (400) is photomultiplier.
According to micro light measuring system provided by the present invention, it utilizes reaction cup to have darkroom of one's own, and good airproof performance is more conducive to flowline operation.
Accompanying drawing explanation
From subsequently, by reference to the accompanying drawings to the description of embodiment, these of exemplary embodiment of the present invention and/or other aspects and advantage will become apparent and be easier to understand, in the accompanying drawings:
Fig. 1 illustrates the schematic diagram of micro light measuring system of the present invention;
Fig. 2 illustrates the schematic diagram of reaction cup of the present invention;
Fig. 3, Fig. 4 illustrate the schematic diagram of pressed sealing device of the present invention;
Fig. 5 illustrates the schematic diagram of guiding device of the present invention.
Embodiment
Below by embodiment, and 1-5 by reference to the accompanying drawings, technical scheme of the present invention is described in further detail.Followingly with reference to accompanying drawing, the explanation of embodiment of the present invention is intended to general plotting of the present invention to make an explanation, and does not should be understood to a kind of restriction of the present invention.
As shown in Figure 1, micro light measuring system comprises reaction cup 100, pressed sealing device 200, guiding device 300 and photometry sensor 400, and described reaction cup 100 is arranged to hold luminescent substance to be detected; Described pressed sealing device 200 has the first connecting portion and the second connecting portion, and described the first connecting portion is connected with described reaction cup 100, and is arranged to make described the first connecting portion and described reaction cup to form darkroom; Described the second connecting portion is connected with described guiding device 300, so that the light of luminescent substance is transferred to described photometry sensor 400 from described guiding device; Described photometry sensor is connected with described guiding device, and described photometry sensor is arranged to detect described light, thereby detects described luminescent substance.
According to a preferred embodiment of the invention, described reaction cup is made up of the light tight material of black, and as shown in Figure 2, described reaction cup is that six cup holes connect in a row.For sealing being provided and contacting effect, described first connecting portion of pressed sealing device 200 comprises caulking gum portion 208, and described caulking gum portion matches with described reaction cup 100.The each glass of hole place of described reaction cup can arrange groove, for contacting with the caulking gum portion sealing of pressed sealing device.
According to a preferred embodiment of the invention, as shown in Figure 3, described the second connecting portion comprises compact heap 207, leaded light sleeve 206 and leaded light fixed block 205, compact heap 207 is arranged to around leaded light sleeve 206, and both can vertically slidably connect, guiding device 300 is through leaded light sleeve 206, and leaded light fixed block 205 is arranged on one end of leaded light sleeve 206, so that guiding device 300 is fixed, the other end of leaded light sleeve 206 is connected with described the first connecting portion.
As shown in Figure 3, pressed sealing device 200 also comprises drive division, and described drive division drives described compact heap vertically to move up and down.Drive division comprises motor 201, slide block 204 and leading screw 203, described slide block 204 is arranged to be connected with described leading screw 203, and be connected with described compact heap 207, described motor 201 drives described leading screw 203 to rotate, leading screw 203 is with movable slider 204 to slide up and down, thereby compact heap 207 moves up and down or slides.For the stability moving up and down is provided, back shaft 203 can also be set, as shown in Figure 3, back shaft 203 is through described slide block 204, and it provides a supporting role for moving up and down of slide block 204.
As shown in Figure 4, in order to improve sealing effectiveness, between described compact heap 207 and described leaded light sleeve 206, spring 209 is set, and described spring is arranged to, in the time that described the first connecting portion does not contact with described reaction cup 100, leaded light sleeve 206 is freely sagging under the effect of the elastic force of described spring; In the time that described the first connecting portion contacts with described reaction cup 100, described spring 209 produces snap-in force on described leaded light sleeve 206, thereby, make to produce snap-in force between described the first connecting portion and described reaction cup.
According to a preferred embodiment of the invention, as shown in Figure 4, with the inwall that leaded light sleeve 206 contacts, recess is set at compact heap 207, with the outer wall that compact heap 207 contacts, protuberance is set at leaded light sleeve 206, this protuberance can slide up and down in this recess, spring 209 is arranged to one end and is contacted with described protuberance, and one end contacts with the end of described recess.Thereby spring 209 acts on respectively described compact heap 207 and described leaded light sleeve 206.In the time that the caulking gum portion 208 of the first connecting portion does not contact with reaction cup 100, leaded light sleeve is automatically sagging under the elastic force effect of spring.Compact heap 207 moves downward under the drive of leading screw 202, promote leaded light sleeve 206 by spring moves downward simultaneously, in the time that the caulking gum portion 208 on it touches reaction cup 100, caulking gum portion 208 can not continue to move downward, now, compact heap 207 continues to move downward Compress Spring 208, thereby makes to produce a snap-in force in caulking gum portion, improves sealing effectiveness.
Preferably, caulking gum portion can be fluororubber, and shore hardness is 30 o'clock best results, and reaction cup 100 can adopt the plastic injection-moulded die sinking of PPS to form.
As shown in Figure 5, according to a preferred embodiment of the invention, guiding device 300 comprises leading bare headed 301, rear leaded light head 302, liquid-core optical fibre 303.Described leading bare headed 301 are contained in leaded light sleeve 206, and lock by leaded light fixed block 205.Rear leaded light head 302 is connected with photometry sensor 400 by screw, for preventing that light leak from need add rubber gasket in junction.
Preferably, described guiding device is liquid-core optical fibre, has good angle of bend and transmitance.
Preferably, described photometry sensor can be photomultiplier, and wavelength is 300nm-650nm.
According to a preferred embodiment of the invention, adopt the embodiment of one of them micro light measuring system, its course of work is as follows: in the time that needs detect reaction cup, reaction cup to be measured is sent to photometry position by other connecting gear, slide block on driven by motor leading screw moves down, slide block drives compact heap to move down, it is by the spring being in contact with it, drive leaded light sleeve and on caulking gum portion move down, after caulking gum portion contacts with reaction cup, motor continues band movable slider and moves down, it passes through Compress Spring, cause to leaded light sleeve and on the snap-in force of caulking gum portion, thereby being compressed, caulking gum portion and reaction cup form a darkroom.Now, start light measurer, light to be measured is transferred to photometry sensor by guiding device and carries out photometry.After photometry finishes, on the slide block on driven by motor leading screw, move, make caulking gum portion and reaction cup leave certain distance, prepare the test in next reaction cup hole.
Although illustrated and described embodiments of the invention; but those skilled in the art can understand; in the situation that not departing from principle of the present invention and marrow, can make variation to described embodiment, protection scope of the present invention is limited in claim and equivalent thereof.
Claims (9)
1. a micro light measuring system, it comprises:
Reaction cup (100), pressed sealing device (200), guiding device (300) and photometry sensor (400),
It is characterized in that,
Described reaction cup (100) is arranged to hold luminescent substance to be detected;
Described pressed sealing device (200) has the first connecting portion and the second connecting portion, and described the first connecting portion is connected with described reaction cup (100), and is arranged to make described the first connecting portion and described reaction cup to form darkroom; Described the second connecting portion is connected with described guiding device (300), so that the light of luminescent substance is transferred to described photometry sensor (400) from described guiding device;
Described photometry sensor is connected with described guiding device, and described photometry sensor is arranged to detect described light, thereby detects described luminescent substance;
Described the second connecting portion comprises compact heap, leaded light sleeve and leaded light fixed block,
Described compact heap is arranged to around described leaded light sleeve, and both can vertically slidably connect,
Described guiding device passes described leaded light sleeve,
Described leaded light fixed block is arranged on one end of described leaded light sleeve, so that described guiding device is fixed,
The other end of described leaded light sleeve is connected with described the first connecting portion.
2. micro light measuring system according to claim 1, wherein, described first connecting portion of described pressed sealing device (200) comprises caulking gum portion, described caulking gum portion matches with described reaction cup (100).
3. micro light measuring system according to claim 2, wherein, described reaction cup (100) comprises groove, described caulking gum portion matches with described groove.
4. micro light measuring system according to claim 1, wherein,
Between described compact heap and described leaded light sleeve, spring is set, and described spring is arranged to, in the time that described the first connecting portion contact with described reaction cup, described leaded light sleeve freedom under the effect of the elastic force of described spring is sagging; In the time that described the first connecting portion contacts with described reaction cup, described spring produces snap-in force on described leaded light sleeve, thereby, make to produce snap-in force between described the first connecting portion and described reaction cup.
5. micro light measuring system according to claim 1, wherein,
Described pressed sealing device (200) also comprises drive division, and described drive division drives described compact heap vertically to move up and down.
6. micro light measuring system according to claim 5, wherein,
Described drive division comprises motor, slide block and leading screw,
Described slide block is arranged to be connected with described leading screw, and is connected with described compact heap,
Described motor drives described leading screw to rotate,
Described leading screw drives described slide block to slide up and down, thereby described compact heap slides up and down.
7. micro light measuring system according to claim 1, wherein,
Described reaction cup is made up of black light-proof material.
8. micro light measuring system according to claim 1, wherein,
Described guiding device (300) comprises leading shaven head, rear leaded light head and liquid-core optical fibre,
Described leading shaven head is for being connected with the second connecting portion of described pressed sealing device;
Described rear leaded light head is for being connected with described photometry sensor;
Described liquid-core optical fibre is for transmitting the light that described luminescent substance sends.
9. micro light measuring system according to claim 1, wherein,
Described photometry sensor (400) is photomultiplier.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210071730.1A CN102608129B (en) | 2012-03-16 | 2012-03-16 | Shimmer detecting system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210071730.1A CN102608129B (en) | 2012-03-16 | 2012-03-16 | Shimmer detecting system |
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| CN102608129A CN102608129A (en) | 2012-07-25 |
| CN102608129B true CN102608129B (en) | 2014-07-09 |
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| CN201210071730.1A Active CN102608129B (en) | 2012-03-16 | 2012-03-16 | Shimmer detecting system |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107576784B (en) * | 2017-08-28 | 2024-01-30 | 重庆科斯迈生物科技有限公司 | Chemiluminescent immunoassay camera bellows detecting system |
| CN108362642B (en) * | 2018-02-09 | 2023-11-17 | 深圳市活水床旁诊断仪器有限公司 | Chemiluminescence immunoassay photometry device and method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201765226U (en) * | 2010-04-06 | 2011-03-16 | 合肥运涛光电科技有限公司 | Dial type direct-reading fully-automatic immune luminescence analysis system |
| CN201945557U (en) * | 2011-01-07 | 2011-08-24 | 博奥赛斯（天津）生物科技有限公司 | Ultra low interference photon collecting system and chemiluminescence immunoassay analyzer comprising same |
| CN202583055U (en) * | 2012-03-16 | 2012-12-05 | 北京勤邦生物技术有限公司 | Shimmer detection system |
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- 2012-03-16 CN CN201210071730.1A patent/CN102608129B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201765226U (en) * | 2010-04-06 | 2011-03-16 | 合肥运涛光电科技有限公司 | Dial type direct-reading fully-automatic immune luminescence analysis system |
| CN201945557U (en) * | 2011-01-07 | 2011-08-24 | 博奥赛斯（天津）生物科技有限公司 | Ultra low interference photon collecting system and chemiluminescence immunoassay analyzer comprising same |
| CN202583055U (en) * | 2012-03-16 | 2012-12-05 | 北京勤邦生物技术有限公司 | Shimmer detection system |
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