Immuno-chromatography detection device
Technical field
The utility model relates to a kind of immuno-chromatography detection device.
Background technology
Immunochromatographic method (immunochromatography) is a kind of quick diagnosis technology of rising in recent years, its principle is a certain district band that special antibody is first fixed on to nitrocellulose filter, when this dry cellulose nitrate one end is immersed after sample (urine or serum), due to capillarity, sample will move forward along this film, in the time moving to the region that is fixed with antibody, in sample corresponding antigen with this antibody generation specific binding, if can make this region show certain color with immune colloid gold or Immunoperoxidase Staining, thereby realize specific immunodiagnosis.
In China's family expenses medical treatment quick diagnosis field, conventionally adopt at present the method for visual inspection to judge the testing result of immuno-chromatographic test paper strip, but this judgement varies with each individual, simultaneously to run into measurand content very low, develop the color very shallow, be difficult to result of determination with the naked eye, cause the erroneous judgement to user health situation and the mistaken diagnosis that causes.Therefore, need especially the accurately device of reading immunity-chromatography test strip testing result.
Number of patent application is method and system and the application thereof that 200910214016.1 Chinese invention patent application discloses a kind of intelligent identification and reading immunity-chromatography test strip, although realized quantitative reading immunity-chromatography test strip testing result, but there are the following problems: 1, operation steps complexity, test strips separates setting with reading device, test strips after colour developing manually need to be placed in reading device and read, between different test strips, easily obscure.2, easily phase mutual interference between the detection light of the surveyed area of test strips and control area, reading result is unreliable.
Summary of the invention
The purpose of this utility model is in order to address the above problem, and a kind of immuno-chromatography detection device is provided.
For achieving the above object, the technical solution of the utility model is as follows:
A kind of immuno-chromatography detection device, comprise housing, be located at the display of surface of shell, be located at the test strips with surveyed area and control area in housing, surveyed area light path cavity, control area light path cavity, circuit of measurement and control, be located at the test strips injection port of shell end, described surveyed area light path cavity is provided with the detection line detection window with ELISA test strip region intercommunication light path, described control area light path cavity is provided with the control line detection window with the control area intercommunication light path of test strips, described test strips is light transmission immuno-chromatographic test paper strip, described surveyed area light path cavity and control area light path cavity are located at the not ipsilateral of test strips, make the light path cavity of surveyed area and the light path cavity of control area separate,
Described circuit of measurement and control comprises microcontroller, surveyed area light source, surveyed area photodetector, control area light source, control area photodetector, light path part, power circuit, described display, light path part, surveyed area light source, surveyed area photodetector, control area light source, control area photodetector and power circuit are connected with microcontroller respectively, described surveyed area light source and surveyed area photodetector are located in surveyed area light path cavity, and described control area light source and control area photodetector are located in the light path cavity of control area.
Preferably, described control area light path cavity is arranged on the top of control area, described surveyed area light path cavity is arranged on the below of surveyed area, or described control area light path cavity is arranged on the below of control area, and described surveyed area light path cavity is arranged on the top of surveyed area.
Preferably, described circuit of measurement and control also comprises the testing circuit for detection of sample validity, and described control area light source is connected with microcontroller by described testing circuit respectively with control area photodetector.
Preferably, described test strips injection port is provided with the suction lining bar being communicated with test strips.
Preferably, described display is LCDs.
Preferably, in described housing, be provided with the sample photodetector for automatically controlling testing circuit.
Preferably, described pick-up unit also comprises lid, and described lid and shell end are removably connected to form airtight cavity, and test strips injection port is sealed in above-mentioned cavity.
Preferably, the profile of described pick-up unit is the form of a stroke or a combination of strokes.
The detection method of the utility model immuno-chromatography detection device comprises the steps:
(1) provide a kind of immuno-chromatography detection device, comprise housing, be located at the display of surface of shell, be located at the test strips with surveyed area and control area in housing, surveyed area light path cavity, control area light path cavity, circuit of measurement and control, be located at the test strips injection port of shell end, described surveyed area light path cavity is provided with the detection line detection window with ELISA test strip region intercommunication light path, described control area light path cavity is provided with the control line detection window with the control area intercommunication light path of test strips, described test strips is light transmission immuno-chromatographic test paper strip, described surveyed area light path cavity and control area light path cavity are located at the not ipsilateral of test strips, make the light path cavity of surveyed area and the light path cavity of control area separate,
Described circuit of measurement and control comprises microcontroller, surveyed area light source, surveyed area photodetector, control area light source, control area photodetector, light path part, power circuit, described display, light path part, surveyed area light source, surveyed area photodetector, control area light source, control area photodetector and power circuit are connected with microcontroller respectively, described surveyed area light source and surveyed area photodetector are located in surveyed area light path cavity, and described control area light source and control area photodetector are located in the light path cavity of control area;
(2) liquid sample is contacted with the test strips injection port of shell end, liquid sample flow in test strips, start detection device;
(3) light source being placed in surveyed area light path cavity irradiates the surveyed area in test strips, and the photodetector being placed in surveyed area light path cavity detects the light reflecting from surveyed area, is converted into electric signal, is sent to microcontroller plate; The light source being placed in the light path cavity of control area irradiates the control area in test strips, the photodetector being placed in the light path cavity of control area detects the light reflecting from control area, be converted into electric signal, be resent to microcontroller, the validity that judgement sample detects;
(4) microcontroller received current signal, carries out conversion process analysis, and Treatment Analysis result is shown by LCDs.
beneficial effect
Compared with prior art, light transmission immuno-chromatographic test paper strip is set in the utility model pick-up unit, utilize the perspectivity of reaction marking thing on nitrocellulose filter, can be above test strips and below respectively surveyed area and control area are detected, the separate setting of light path cavity of the light path cavity of surveyed area and control area, completely cut off surveyed area and control area completely and detected the phase mutual interference between light, strengthened the reliability of testing result.Test strips and pick-up unit are wholely set, and test strips is drawn after sample at once start detection device automatically, after several minutes, testing result can be shown to user by LCDs, and operation is very easy, and various users use unrestricted, meet family and use.The miniaturization of the utility model product, cost is low, is easy to produce, and simple to operate, show result quantification and directly perceived.
Brief description of the drawings
Fig. 1 is the structural drawing installing described in the utility model embodiment;
Fig. 2 is the structural drawing that installs use state described in the utility model embodiment;
Fig. 3 is the cut-open view installing described in the utility model embodiment;
Fig. 4 is the principle of work block scheme installing described in the utility model embodiment.
Embodiment
For making that architectural feature of the present utility model and effect of reaching are had a better understanding and awareness, coordinate detailed explanation in order to preferred embodiment and accompanying drawing, be described as follows:
As shown in Figure 1, Figure 2, Figure 3 shows, immuno-chromatography detection device, comprise housing 2, the removably lid 1, the LCDs 3 of being located at surface of shell, test strips 5 and the circuit of measurement and control of being located at enclosure interior that are connected with housing, the end of housing is provided with the water suction rod 4 for sample introduction, the excellent one end of described water suction contacts with the test strips in housing, the excellent other end of described water suction extends housing from enclosure interior to shell end, is placed in the chamber that lid and shell end form.In use, lid is taken off from housing, the water suction rod of shell end is immersed in liquid sample, liquid sample is flow in test strips along water suction rod.
Described test strips 5 is immuno-chromatographic test paper strips of light transmission, utilizes the perspectivity of reaction marking thing on nitrocellulose filter.Described test strips 5 has surveyed area 14 and control area 15, above or below surveyed area, be provided with surveyed area light path cavity 6, above or below control area, be provided with control area light path cavity 7, and surveyed area light path cavity 6 and control area light path cavity 7 are arranged on the not ipsilateral of test strips 5, specifically, if control area light path cavity 7 is arranged on the top of control area, surveyed area light path cavity 6 is arranged on the below of surveyed area, if or control area light path cavity 7 is arranged on the below of control area, surveyed area light path cavity 6 is arranged on the top of surveyed area.7 li of surveyed area light path cavity 6 and control area light path cavitys arrange respectively separate light source and photodetector, described surveyed area light path cavity is provided with the detection line detection window 8 with ELISA test strip region intercommunication light path, described control area light path cavity is provided with the control line detection window 9 with the control area intercommunication light path of test strips, arrange like this, in the time detecting, can avoid surveyed area and control area to detect the phase mutual interference between light completely.
As shown in Figure 4, described circuit of measurement and control comprises microcontroller 16, surveyed area light source 10, surveyed area photodetector 11, control area light source 12, control area photodetector 13, light path part, power circuit, testing circuit 17, described LCDs 3, light path part, surveyed area light source 10, surveyed area photodetector 11, testing circuit 17 is connected with microcontroller respectively with power circuit, described control area light source 12 is connected with microcontroller 16 by testing circuit 17 with control area photodetector 13, described surveyed area light source 10 and surveyed area photodetector 11 are located in surveyed area light path cavity 6, described control area light source 12 and control area photodetector 13 are located in control area light path cavity 7.
As shown in Figure 3, when liquid sample flows to after test strips along water suction rod (direction of arrow in figure), by the sample photodetector arranging, after the sample detecting in test strips, send a signal to microcontroller in housing, thus start detection device.
Light source in microprocessor controls surveyed area light path cavity irradiates the surveyed area in test strips, and the photodetector being placed in surveyed area light path cavity detects the light reflecting from surveyed area, is converted into electric signal, is sent to microcontroller plate; The light source being placed in the light path cavity of control area irradiates the control area in test strips, the photodetector being placed in the light path cavity of control area detects the light reflecting from control area, be converted into electric signal, be resent to microcontroller, the validity that judgement sample detects.Microcontroller received current signal, carries out conversion process analysis, and Treatment Analysis result is shown by LCDs.Treatment Analysis result can be quantitative analysis results or the qualitative analysis.
The detection method of the utility model immuno-chromatography detection device, comprises the steps:
1, provide a kind of immuno-chromatography detection device.
2, liquid sample is contacted with the test strips injection port (water suction rod) of shell end, liquid sample flow in test strips, automatically start detection device.
3, the light source being placed in surveyed area light path cavity irradiates the surveyed area in test strips, and the photodetector being placed in surveyed area light path cavity detects the light reflecting from surveyed area, is converted into electric signal, is sent to microcontroller plate; The light source being placed in the light path cavity of control area irradiates the control area in test strips, the photodetector being placed in the light path cavity of control area detects the light reflecting from control area, be converted into electric signal, be resent to microcontroller, the validity that judgement sample detects.
4, microcontroller received current signal, carries out conversion process analysis, and Treatment Analysis result is shown by LCDs.
More than show and described the advantage of ultimate principle, principal character and the utility model of utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; what in above-described embodiment and instructions, describe is principle of the present utility model; under the prerequisite that does not depart from the utility model spirit and scope, the utility model also has various changes and modifications, and these changes and improvements all fall in claimed scope of the present utility model.The protection domain that the utility model requires is defined by appending claims and equivalent thereof.