CN102604912B - 高活力华根霉脂肪酶突变体 - Google Patents
高活力华根霉脂肪酶突变体 Download PDFInfo
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- CN102604912B CN102604912B CN 201210083915 CN201210083915A CN102604912B CN 102604912 B CN102604912 B CN 102604912B CN 201210083915 CN201210083915 CN 201210083915 CN 201210083915 A CN201210083915 A CN 201210083915A CN 102604912 B CN102604912 B CN 102604912B
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Description
脂肪酶突变体 | 氨基酸取代位置 | K cat(/min) | K cat提高的倍数 |
出发菌株脂肪酶 | - | 1138 | 1.0 |
突变体1-1 | Ala129Ser | 1252 | 1.1 |
突变体1-2 | Lys161Arg | 1479 | 1.3 |
突变体1-3 | Thr195Tyr | 1479 | 1.3 |
突变体1-4 | Ala230Thr | 1821 | 1.6 |
突变体1-5 | Val261Gly | 1935 | 1.7 |
突变体1-6 | Lys322Arg | 1366 | 1.2 |
突变体1-7 | Ser373Asn | 1707 | 1.5 |
突变体2-1 | Ala129Ser / Lys161Arg | 2278 | 2 |
突变体2-2 | Ala129Ser / Ala230Thr | 1365 | 1.2 |
突变体2-3 | Ala129Ser / Val261Gly | 1593 | 1.4 |
突变体2-4 | Lys161Arg/Thr195Tyr | 2845 | 2.5 |
突变体2-5 | Lys161Arg / Lys322Arg | 1707 | 1.5 |
突变体2-6 | Lys161Arg / Ser373Asn | 2048 | 1.8 |
突变体2-7 | Ala230Thr /Ser373Asn | 1707 | 1.5 |
突变体2-8 | Val261Gly/ Ser373Asn | 3726 | 3.3 |
突变体3-1 | Ala129Ser/Ala230Thr /Val261Gly | 3186 | 2.8 |
突变体3-2 | Ala129Ser/Ala230Thr/Lys322Arg | 2278 | 2 |
突变体3-3 | Lys161Arg/Thr195Tyr/Ser373Asn | 2617 | 2.3 |
突变体3-4 | Lys161Arg/Ala230Thr/Ser373Asn | 2278 | 2 |
突变体3-5 | Lys161Arg/Val261Gly/Lys322Arg | 2731 | 2.4 |
突变体3-6 | Ala230Thr/Lys322Arg/Ser373Asn | 2845 | 2.5 |
突变体4-1 | Ala129Ser/Lys161Arg/Ala230Thr/ Lys322Arg | 2073 | 1.8 |
脂肪酶突变体 | 氨基酸取代位置 | K cat(/min) | K cat提高的倍数 |
出发菌株脂肪酶 | - | 1138 | 1.0 |
突变体2-1 | Ala129Ser / Lys161Arg | 2278 | 2 |
突变体2-4 | Lys161Arg/Thr195Tyr | 2845 | 2.5 |
突变体2-7 | Ala230Thr /Ser373Asn | 1707 | 1.5 |
突变体2-8 | Val261Gly/ Ser373Asn | 3726 | 3.3 |
突变体3-2 | Ala129Ser/Ala230Thr/Lys322Arg | 2278 | 2 |
突变体3-4 | Lys161Arg/Ala230Thr/Ser373Asn | 2278 | 2 |
脂肪酶突变体 | 氨基酸取代位置 | K cat(/min) | K cat提高的倍数 |
出发菌株脂肪酶 | - | 1138 | 1.0 |
突变体1-1 | Ala129Ser | 1252 | 1.1 |
突变体1-2 | Lys161Arg | 1479 | 1.3 |
突变体1-3 | Thr195Tyr | 1479 | 1.3 |
突变体1-4 | Ala230Thr | 1821 | 1.6 |
突变体1-5 | Val261Gly | 1935 | 1.7 |
突变体1-6 | Lys322Arg | 1366 | 1.2 |
突变体1-7 | Ser373Asn | 1707 | 1.5 |
突变体2-2 | Ala129Ser / Ala230Thr | 1365 | 1.2 |
突变体2-3 | Ala129Ser / Val261Gly | 1593 | 1.4 |
突变体2-5 | Lys161Arg / Lys322Arg | 1707 | 1.5 |
突变体2-6 | Lys161Arg / Ser373Asn | 2048 | 1.8 |
突变体3-1 | Ala129Ser/Ala230Thr /Val261Gly | 3186 | 2.8 |
突变体3-3 | Lys161Arg/Thr195Tyr/Ser373Asn | 2617 | 2.3 |
突变体3-5 | Lys161Arg/Val261Gly/Lys322Arg | 2731 | 2.4 |
突变体3-6 | Ala230Thr/Lys322Arg/Ser373Asn | 2845 | 2.5 |
突变体4-1 | Ala129Ser/Lys161Arg/Ala230Thr/ Lys322Arg | 2073 | 1.8 |
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CN 201210083915 CN102604912B (zh) | 2009-11-11 | 2009-11-11 | 高活力华根霉脂肪酶突变体 |
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CN 201210083915 CN102604912B (zh) | 2009-11-11 | 2009-11-11 | 高活力华根霉脂肪酶突变体 |
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CN2009102351853A Division CN101899427B (zh) | 2009-11-11 | 2009-11-11 | 通过定向进化构建的活力提高的脂肪酶突变体 |
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CN102604912A CN102604912A (zh) | 2012-07-25 |
CN102604912B true CN102604912B (zh) | 2013-03-13 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014147127A1 (en) * | 2013-03-21 | 2014-09-25 | Novozymes A/S | Polypeptides with lipase activity and polynucleotides encoding same |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2015004102A1 (en) * | 2013-07-09 | 2015-01-15 | Novozymes A/S | Polypeptides with lipase activity and polynucleotides encoding same |
WO2015091746A1 (en) | 2013-12-20 | 2015-06-25 | Novozymes A/S | Compositions and processes for treatment with lipases |
WO2015109972A1 (en) * | 2014-01-22 | 2015-07-30 | Novozymes A/S | Polypeptides with lipase activity and polynucleotides encoding same |
CN105200024B (zh) * | 2015-08-24 | 2018-09-21 | 安徽丰原发酵技术工程研究有限公司 | 脂肪酶calb突变体、其制备方法及应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1681942A (zh) * | 2002-09-13 | 2005-10-12 | 韩国生命工学研究院 | 使用酵母表面展示载体筛选具有改良的酶活性的脂肪酶的方法和该脂肪酶 |
CN101544966A (zh) * | 2009-04-28 | 2009-09-30 | 江南大学 | 一种重组酵母脂肪酶的发酵制备方法 |
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2009
- 2009-11-11 CN CN 201210083915 patent/CN102604912B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1681942A (zh) * | 2002-09-13 | 2005-10-12 | 韩国生命工学研究院 | 使用酵母表面展示载体筛选具有改良的酶活性的脂肪酶的方法和该脂肪酶 |
CN101544966A (zh) * | 2009-04-28 | 2009-09-30 | 江南大学 | 一种重组酵母脂肪酶的发酵制备方法 |
Non-Patent Citations (4)
Title |
---|
Improved thermostability and the optimum temperature of Rhizopus arrhizus lipase by directed evolution;Wei-Ning Niu等;《Journal of Molecular Catalysis B:Enzymatic》;20061231;第43卷;第33-39页 * |
Wei-Ning Niu等.Improved thermostability and the optimum temperature of Rhizopus arrhizus lipase by directed evolution.《Journal of Molecular Catalysis B:Enzymatic》.2006,第43卷 |
基于易错PCR技术的短小芽孢杆菌YZ02脂肪酶基因BpL的定向进化;黄瑛等;《生物工程学报》;20080325;第24卷(第3期);第445-451页 * |
黄瑛等.基于易错PCR技术的短小芽孢杆菌YZ02脂肪酶基因BpL的定向进化.《生物工程学报》.2008,第24卷(第3期), |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014147127A1 (en) * | 2013-03-21 | 2014-09-25 | Novozymes A/S | Polypeptides with lipase activity and polynucleotides encoding same |
CN105051174A (zh) * | 2013-03-21 | 2015-11-11 | 诺维信公司 | 具有脂肪酶活性的多肽和编码它们的多核苷酸 |
US9631164B2 (en) | 2013-03-21 | 2017-04-25 | Novozymes A/S | Polypeptides with lipase activity and polynucleotides encoding same |
CN105051174B (zh) * | 2013-03-21 | 2018-04-03 | 诺维信公司 | 具有脂肪酶活性的多肽和编码它们的多核苷酸 |
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